CN106435046A - Method for extracting fructose from sunflower head and stem pith - Google Patents
Method for extracting fructose from sunflower head and stem pith Download PDFInfo
- Publication number
- CN106435046A CN106435046A CN201610808389.1A CN201610808389A CN106435046A CN 106435046 A CN106435046 A CN 106435046A CN 201610808389 A CN201610808389 A CN 201610808389A CN 106435046 A CN106435046 A CN 106435046A
- Authority
- CN
- China
- Prior art keywords
- sunflower
- fructose
- column chromatography
- filter
- ethanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K11/00—Fructose
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a method for extracting fructose from sunflower head and stem pith. The method comprises the following steps: using sunflower head and stem pith after harvesting sunflower seeds as raw materials; extracting with ethanol; carrying out filtration, concentration, column chromatography and other methods to obtain the fructose. The fructose prepared by the invention is derived from natural components of the sunflower, does not contain any added substances and can be widely applied to healthy food, beverage and other food industries. The method is simple in process, high in yield, low in cost, and easy in industrial production, can turn waste into treasure, and realizes deep processing and reuse of sunflower byproducts.
Description
Technical field
The present invention relates to a kind of method extracting fructose from sunflower plate and stem marrow, specifically, the present invention relates to sharp
Method with fructose in water or solvent extraction, column chromatography technology separation sunflower plate and stem marrow.
Background technology
Sunflower, in China's plantation history of existing more than 400 year, is one of main oil crops of China, China northeast,
The ground such as northwest and Inner Mongol is extensively planted, abundance.During sunflower pick and process, Main By product is certain herbaceous plants with big flowers disk and stalk, this
A little accessory substance major parts in addition to small part producing fodder are taken as log even to go out of use.This not only have impact on sunflower industry
The lifting of comprehensive benefit, more causes the waste of resource and the pollution of environment.Therefore, the deep processing profit of sunflower plate and stalk
With being respectively provided with significance for the utilization rate improving resource and the overall economic efficiency of sunflower and environmental protection.Fructose
It is the isomer of glucose, compared with glucose, have and soon, easily absorb in human body metabolism and be independent of the spies such as insulin
Point, is suitable to the patient of glucose metabolism and dyshepatia；Fructose may also suppress the excess accumulation of body fat, promotes the suction of calcium
Receive.Additionally, fructose sugariness is 1.8 times of sucrose, and to carious tooth, there is certain inhibitory action, can be widely applied to beverage, cake
The industry of the relevant food such as point, has great market application foreground.The method extracting fructose from sunflower plate and stem marrow, so far
There is not been reported.We are studied in the extraction to fructose in sunflower plate and stem marrow first, the method process is simple, yield
Height, with low cost it is easy to industrialized production.
Content of the invention
1 is to improve the utilization rate of sunflower accessory substance and economic worth, one of the object of the invention there are provided a kind of from
The method preparing fructose in sunflower plate and stem marrow, comprises the following steps that：
(1) sorting：Choose and sunflower plate and stem marrow be dried after harvesting sunflower seeds, remove moldy metamorphism raw material.
(2) pulverize：Sunflower plate or stem marrow raw material are pulverized, crosses 10～100 mesh sieves.
(3) extract：The dry material pulverized is compared 1 by feed liquid (mass/volume):10～100 ratio adds edible ethanol
(concentration is 50～100%), temperature is room temperature or heating (30～90 DEG C), and mode is extraction, or seepage pressure effects, or microwave
Or ultrasonic assistant extracts, extraction time is different because of extracting mode, about 0.5～48h.
(4) filter：Using methods such as gauze, filter cloth, filters (pore size filter 0.1～1.0mm), extract was carried out
Filter, obtains sunflower fructose crude extract.
(5) concentrate：Carry out the concentration of sunflower extract using reduced pressure concentration method, temperature setting is 40～80 DEG C, obtains
Sunflower plate or stem marrow extract medicinal extract.
(6) column chromatography：With 100～300 mesh silica gel after above-mentioned medicinal extract is dissolved with edible ethanol (concentration is for 50～100%)
Mix sample, after mixed sample low temperature drying (30～60 DEG C), carry out silica gel column chromatography, eluting solvent is food-grade n-hexane and food
With absolute ethyl alcohol, the ratio that elutes is 0:100～100:0；Fractional Collections eluent, after TLC identification, merges and has fruit
The component of sugar simultaneously carries out reduced pressure concentration；Concentrate carries out macroporous adsorbent resin column chromatography with after pure water dilution, and eluting solvent is
Pure water and edible absolute ethyl alcohol, wash-out ratio is 0:100～100:0；Fractional Collections eluent.
(7) after TLC identification, the component that merging has fructose carries out sunflower using reduced pressure concentration method to eluent
The concentration of fructose liquid, temperature setting is 40～80 DEG C, obtains sunflower fructose.、
Brief description
Fig. 1 is a kind of process chart of the method extracting fructose from sunflower plate and stem marrow of the present invention
Specific embodiment
In conjunction with but be not limited to following specific embodiments the present invention will be described.The essence of the present invention and covering scope are by weighing
Sharp claim limits.For the field of place of the present invention, on the premise of without departing substantially from present invention essence and range of application, to invention
Sunflower raw material in content, consumption and obtain through refining the various changes that the preparation technologies such as solvent carry out also in the protection model of the present invention
Within enclosing.Embodiment 1：
A kind of preparation method of sunflower fructose, comprises the following steps that：
(1) sorting：The sunflower stem pith drying after choosing harvesting sunflower seeds, removes moldy metamorphism raw material.
(2) pulverize：20 mesh sieves are crossed after the sunflower stem pith chosen raw material 1.0kg is pulverized.
(3) extract：The raw material of pulverizing is poured in 40.0L absolute ethyl alcohol, filters after 50 DEG C of refluxing extraction 3.0h, filter residue is again
Pour in 40.0L absolute ethyl alcohol, carry out second extraction；Repeatedly extract and amount to 3 times.
(4) filter：By extract with 100 mesh filter-cloth filterings, collect filtrate and concentrated.
(5) concentrate：The filtrate obtaining in step (4) is carried out reduced pressure concentration with 5.0L Rotary Evaporators, temperature setting is
50 DEG C, obtain stem marrow medicinal extract about 80.0g.
(6) column chromatography：Above-mentioned medicinal extract is waited mixed sample with after edible anhydrous alcohol solution with 100～300 mesh silica gel mixed samples
Product carry out silica gel column chromatography after being dried, and eluting solvent is food-grade n-hexane and edible absolute ethyl alcohol, and wash-out ratio is 0:100～
100:0；Collecting, merging wash-out concentration is 10:90～0:100 efflux, obtains having the component of fructose about after reduced pressure concentration
61.5g；Again with, after pure water about 300.0mL dissolving, carrying out macroporous adsorbent resin column chromatography, eluting solvent is pure water and eats
Absolute ethyl alcohol, wash-out ratio is 0:100～100:0, collect, merging wash-out concentration is water：Ethanol=30:70～10:90 contain
Fructose efflux.
(7) concentrate：The fructose liquid obtaining in step (6) is carried out reduced pressure concentration, temperature setting with 5.0L Rotary Evaporators
For 50 DEG C, after reduced pressure concentration, obtain sunflower fructose 50.1g.
Claims (7)
1. a kind of method of fructose of extracting from sunflower plate and stem marrow is it is characterised in that comprise the steps：(1) sorting：Choosing
Take remove seed the certain herbaceous plants with big flowers disk that dries and stem marrow after sunflower harvesting, remove moldy metamorphism raw material；(2) pulverize：By sunflower plate or stem
Marrow raw material is pulverized, and crosses 10～200 mesh sieves；(3) extract；(4) filter；(5) concentrate；(6) column chromatography；(7) obtain to day after concentrating
Certain herbaceous plants with big flowers fructose.
2. method according to claim 1 is it is characterised in that the extraction process of step (3)：Solvent is that edible ethanol is (dense
Spend for 50～100%), feed liquid mass volume ratio (g/mL) is 1:10～100, temperature is room temperature or heating (20～90 DEG C), when
Between be 0.5～48h, mode is extraction or seepage pressure effects and microwave or ultrasonic assistant extracts.
3. method according to claim 1 is it is characterised in that the filter process of step (4)：Using gauze, filter cloth, filtration
The methods such as device (pore size filter 0.1～1.0mm) filter to extract.
4. method according to claim 1 is it is characterised in that the concentration process of step (5)：Using reduced pressure concentration, temperature
It is set to 40～80 DEG C.
5. method according to claim 1 is it is characterised in that the column chromatography procedure of step (6)：Adopt silica gel column layer successively
Analysis carries out fructose with macroporous adsorbent resin column chromatography and separates；The solvent of wherein silica gel column chromatography is food-grade n-hexane and edible second
(gradient elution ratio is n-hexane to alcohol：Ethanol=100:0～0:100) solvent that, macroporous adsorbent resin column chromatography uses is pure
(gradient elution ratio is water for water purification and edible ethanol：Ethanol=100:0～0:100).
6. method according to claim 1 is it is characterised in that the concentration process of step (7)：Using reduced pressure concentration, temperature
It is set to 40～80 DEG C.
7. method according to claim 1 is it is characterised in that step (7) obtains sunflower fructose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610808389.1A CN106435046A (en) | 2016-09-07 | 2016-09-07 | Method for extracting fructose from sunflower head and stem pith |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610808389.1A CN106435046A (en) | 2016-09-07 | 2016-09-07 | Method for extracting fructose from sunflower head and stem pith |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106435046A true CN106435046A (en) | 2017-02-22 |
Family
ID=58165365
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610808389.1A Pending CN106435046A (en) | 2016-09-07 | 2016-09-07 | Method for extracting fructose from sunflower head and stem pith |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106435046A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102808046A (en) * | 2012-08-09 | 2012-12-05 | 宁波杰顺生物科技有限公司 | Method for extracting fructose from apple pomace |
-
2016
- 2016-09-07 CN CN201610808389.1A patent/CN106435046A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102808046A (en) * | 2012-08-09 | 2012-12-05 | 宁波杰顺生物科技有限公司 | Method for extracting fructose from apple pomace |
Non-Patent Citations (1)
| Title |
|---|
| 黄绪堂: "《黑龙江向日葵》", 31 January 2015, 金盾出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2907395B1 (en) | Methods for manufacturing substance containing nobiletin and tangeretin derived from citrus fruits | |
| CN104177370B (en) | A kind of method preparing high-load sesamin from sesame seed meal | |
| CN105693781A (en) | Technology for extracting tea polyphenol in tea leaves | |
| CN109293712A (en) | The industrialized utilization method and its steviol glycoside and chlorogenic acid of a kind of STEVIA REBAUDIANA | |
| CN102106928B (en) | Method for preparing high-purity oil tea saponins | |
| CN112791126B (en) | Peony pistil extract and preparation method and application thereof | |
| CN102675398B (en) | A kind of method extracting momordica grosvenori glycoside V and farnesol from Grosvenor Momordica | |
| CN100439319C (en) | Method for preparing salviol acid A | |
| CN104262446B (en) | The method of mogroside Ⅴ is extracted from Grosvenor Momordica | |
| CN106668235A (en) | Seedless roxburgh rose general flavone, as well as preparation method and application thereof | |
| CN101659793A (en) | Method for extracting gynura bicolor purpurin | |
| CN103467262B (en) | Method for preparing 9-oxonerolidol from camphor tree plants | |
| CN109021042B (en) | Method for extracting high-purity oleuropein from olive leaves | |
| CN107298642A (en) | A kind of method for extraction and purification of 6 salad oil | |
| CN101560155B (en) | Method for purifying cynarin in artichoke | |
| CN102772490B (en) | Method for preparing paper mulberry staminate inflorescence hard capsule | |
| CN102716255A (en) | Eucommia ulmoides medicinal wine and preparation method thereof | |
| CN104945450A (en) | Method for extracting stibene glucoside from vines of multiflower knotweeds | |
| CN106916065B (en) | Method for preparing high-purity chlorogenic acid from burdock roots | |
| CN106435046A (en) | Method for extracting fructose from sunflower head and stem pith | |
| CN107213180B (en) | Separation and extraction method of notoginseng flavone | |
| CN101810317B (en) | Preparation method of canophyllic polyphenol and application thereof | |
| CN113662974A (en) | Preparation and purification method of water chestnut shell polyphenol extract | |
| CN102952002A (en) | Method for refining magnolol through supersonic extraction separating technology | |
| CN104606264A (en) | Extraction method of sterculia nobilis polyphenol |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170222 |
|
| WD01 | Invention patent application deemed withdrawn after publication |