CN106434614A - Bagasse immobilized bacterium and application thereof to sulfamethoxazole pollution remediation of farmland soil - Google Patents
Bagasse immobilized bacterium and application thereof to sulfamethoxazole pollution remediation of farmland soil Download PDFInfo
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- CN106434614A CN106434614A CN201610912546.3A CN201610912546A CN106434614A CN 106434614 A CN106434614 A CN 106434614A CN 201610912546 A CN201610912546 A CN 201610912546A CN 106434614 A CN106434614 A CN 106434614A
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
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Abstract
The invention discloses a bagasse immobilized bacterium and application thereof to sulfamethoxazole pollution remediation of farmland soil. According to the bagasse immobilized bacterium, Enterobacter cloacae is immobilized by adopting bagasse, and when the Enterobacter cloacae is inoculated to a bagasse vector, a bacterial suspension is prepared by culturing in advance, and then the Enterobacter cloacae is immobilized onto the bagasse vector treated by an inorganic salt culture solution to establish an efficient and stable agricultural waste immobilized bacterium system. The bagasse immobilized bacterium is low in cost, and when the soil is remediated, simplicity and environment-friendliness are achieved, and secondary pollution is avoided; in addition, the bioavailability of sulfonamides can be improved, and the microbiological degradation efficiency of the sulfonamides is improved.
Description
【Technical field】
The present invention relates to bagasse immobilization bacterium and its application in agricultural land soil sulfamethoxazole pollution amelioration.
【Background technology】
Sulfa antibiotics are one of most widely used anti-infectives in livestock breeding industry, its has a broad antifungal spectrum, property
Stable, low price, there is good effect to Perceived control metachromia epidemic disease, at home and abroad have extensive use.In recent decades,
International market is relatively stable always to the demand of sulfa drugs.It is in particularly more poor country of the developing countries
It is always the main force of anti-inflammation, large usage quantity.In developed country, sulfa drugs is then mostly as livestock and poultry treatment and prevention
Medication is added in feed, large usage quantity and demand is relatively stable.China develops rapidly with aquaculture, and sulfa antibiotics make
Consumption also grows with each passing day, and the phenomenon of aquaculture abuse of antibiotics simultaneously is universal.Sulfa antibiotics pass through two channels and enter farmland,
One be feces of livestock and poultry and fish pond mud as organic fertilizer application to farmland, two be in excrement antibiotic through rainfall runoff and plant
Discharge of wastewater etc. enters water body, and is used for field irrigation.In soil, the sulfa antibiotics of a large amount of accumulations for a long time, lead to soil sulphur
Amine antibiotic is seriously polluted.
In some scale pigs and cattle plant swine excrements of China, sulfa antibiotics are up to 13399.5 μ g/kg, high in cow dung
Reach 15930.3 μ g/kg.Some Vegetable Base soil sulfa antibiotics recall rates reach 100.0%.According to the literature 2010 I
The discharge capacity of state's feces of livestock and poultry will reach 4.50 × 109Ton, if according to process level at this stage, about more than 80% feces of livestock and poultry
Without integrated treatment, a part directly imposes on farmland.The nineties in last century, under meat, egg, the promotion of milk demand, China
Livestock and poultry breeding industry develops rapidly, substantial amounts of feeding antibiotic class medicine, is widely used in livestock and poultry breeding industry, and in fast development
Situation.In recent years, feeding antibiotic annual consumption has reached 6.00 × 103Ton, and concentrate and be applied to developed area.
Because sulfa antibiotics have the features such as difficult degradation and high residue, therefore, accumulate in a large number for a long time in agricultural land soil
Sulfa antibiotics not only can affect plant growth, microbiologic population composition and reduce edaphon to other pollutants
Degradation capability, simultaneously the antibiotic of a large amount of uptake and accumulation of crops can by food chain enter human body, lead to human kidney damage,
Allergic reaction, suppression hemopoietic system and resistance etc., constitute to human health and greatly threaten.
In view of the seriousness of antibiotic pollution and harmfulness in environment, antibiotic pollution remediation technology receives extensive pass
Note.In water body and feces of livestock and poultry, middle sulfa antibiotics pollution removal technology research is more in recent years.For example：
Harbin Institute of Technology Jiang this super filter out one plant from mud and can be grown for sole carbon source with sulfamethoxazole
Psychrophile HA-4.Identified bacterial strain HA-4 belongs to Pseudomonas Pseudomonas.The optimum growh degradation condition of bacterial strain HA-4 is：pH
=6.0 about, temperature range is 10 DEG C -15 DEG C, and shaking speed is 150r/min, and inoculum concentration is 15% (V/V), and in this condition
Under, cultivate 192h, the degradation rate of 100mg/l sulfamethoxazole is 34.3%.
Using simple physico-chemical process mainly for a large amount of acid-bearing wastewaters discharged in sulfanilamide (SN) production process, using suitable excessive
Carbide slag is added in acid-bearing wastewater, and continuously stirred 30min can reach and reacts completely, after making acid waste water be in neutral process
Waste water reaches industrial wastewater discharge standard；Using sulphadiazine in ultrasonotomography method degrading waste water, it is 400W in ultrasonic power
Under the conditions of, after ultrasonic 180min, sulphadiazine degradation rate reaches 30.90%；Processed using activated sludge process anaerobic bio-treated
The research of waste water containing sulfanilamide, after waste water containing sulfanilamide dilutes 20 times, then carry out the Air Exposure of more than 26h etc..
Published patent CN201310734659.5 is described by being mixed feces of livestock and poultry with conditioners such as stalk, sawdusts
The aerobic compost method closing, degraded remains in the sulfa antibiotics in feces of livestock and poultry and urine.
CN201510989751.5 describes a kind of method that utilization normal temperature fermentation removes residual antibiotic in feces of livestock and poultry,
First adding the concentrated sulfuric acid to adjust pH in feces of livestock and poultry, then excrement is being laid in closing or semienclosed container, controls temperature
Between 28-35 DEG C, solid state fermentation 4-6 days, remove sulfa antibiotics etc..
Art methods are relatively fast to the degradation rate of the sulfa antibiotics in pig manure, and antibiotic is once enter
, be there is suction-operated first with soil and part antibiotic can be with soil organism formation stable state, mistake under certain condition in soil
Active Bound residues so that in soil sulfa antibiotics there is the features such as difficult degradation and high residue, relevant soil sulfamido
The report of antibiotic pollution amelioration is little.
【Content of the invention】
The invention aims to overcoming the deficiencies in the prior art, provide that a kind of bacterial density is big, reaction speed is fast, resistance to ring
Border impact can remove the bagasse fixation cell of high residue difficult degradation sulfa antibiotics sulfamethoxazole pollution in soil simultaneously
Bacterium.
It is a further object of the present invention to provide a kind of above-mentioned bagasse immobilization bacterium is in soil sulfamethoxazole pollution amelioration
In application.
The present invention is achieved by the following technical solutions：
Bagasse immobilization bacterium is it is characterised in that be carrier immobilized enterobacter cloacae Pseudomonas using bagasse.
With bagasse as carrier in the present invention, build the agricultural residue immobilization bacterium system of efficient stable.Agricultural residue is solid
Surely change bacterium and not only have that bacterial density is big, reaction speed is fast and the features such as resistance to environmental impact, and agricultural residue carrier cheap and
Environmental protection, therefore, has bigger application potential in agricultural land soil sulfa antibiotics pollution amelioration.Concurrently disinfect high residual in soil
Make things difficult for degraded sulfa antibiotics pollution, significant to protecting agriculture production, ecological environment and health.
Microorganism remediation is a kind of method of generally acknowledged effective, safe, cheap and non-secondary pollution.And immobilized microorganism
Technology is primarily referred to as the area of space disperseing free microorganism to be fixed on restriction by method physically or chemically, to improve
The concentration of microbial cell is so as to keep higher biologically active the method recycled.Immobilized microorganism is due to having
Microbe density is big, reaction speed is fast and resistance to environmental impact feature, and therefore, this technology is expected to overcome and is added in reparation scene
The harmful competition of free efficient degradation microorganism and indigenous bacterium or the problem being difficult in adapt to environment,
But the favourable microenvironment that microorganism remediation immobilized microorganism technique is provided by the use of carrier material is as buffer body
System, the shielding Competition of indigenous microorganism and the disoperation of unfavorable edaphic condition, thus ensure that the efficient degradation inoculated is micro-
Biological good growth；The sulfa antibiotics that fixation support can also effectively in Enriching soil as adsorbent simultaneously,
Improve its biologically effective concentration on carrier；In addition the ectoenzyme of microorganism and its secretion is also fixed on carrier by enrichment,
Increased the contacting efficiency with efficient degradation microorganism.Realize the effective repairing polluted soil of efficient degrading bacteria.Micro- for this immobilization
Biological prosthetic organic polluted soil just receives more and more attention.
It mostly is relatively costly organic carrier, such as glutaraldehyde, calcium alginate, sea currently used as microbial immobilized carrier
Mosanom, polyvinyl alcohol and polyacrylamide gel etc. are it is difficult to adapt to the requirement of industrialized production.Agricultural wastes (bagasse, flower
Raw shell and stalk etc.) as microbial immobilized carrier, the competition of indigenous microorganism and unfavorable soil bar not only can be shielded
The infringement of part and then ensure the micro- raw growth of its load, the sulfa antibiotics pollutant in also adsorbable Enriching soil, thus
Increase the bioavailability of sulfa antibiotics, improve the effect of fixing microbial degradation sulfa antibiotics in its surface
Rate.Agricultural wastes contain the nutrients such as abundant C, N；Natural organic matter can be discharged to soil, improve soil physico-chemical property,
Promote soil microbial activities and quantity, strengthen sulfa antibiotics and remove；There is very strong compatibility to microorganism；In soil
Can natural degradation, with after process simple and environmentally-friendly, do not result in secondary pollution；And it is cheap.
Bagasse in the present invention is processed through inorganic salt liquid nutrient solution, during process, inorganic salt liquid nutrient solution and bagasse
Amount ratio be 10mL：3g.
Enterobacter cloacae in the present invention is prepared into bacteria suspension respectively through culture in advance when being inoculated in bagasse carrier.This
Enterobacter cloacae in invention is by being collected in the pig manure chosen property enrichment culture on Guangzhou breeding pig farm, isolate and purify and obtain.
Bacteria suspension in the present invention is 1mL with the amount ratio of the bagasse processing：1～6g, preferably 1mL：3g.
Bagasse is biological material, and the bagasse carrier of biological material bagasse immobilization bacterium system not only can shield soil
Write the competition of microorganism and the infringement of unfavorable edaphic condition and then ensure micro- growth of its load, in also adsorbable Enriching soil
Sulfa antibiotics pollutant, thus increasing the bioavailability of sulfa antibiotics, improve fixing in its surface
The efficiency of microbial degradation sulfa antibiotics.Biological material bagasse is agricultural wastes, containing the nutrition such as abundant C, N unit
Element；Natural organic matter can be discharged to soil, improve soil physico-chemical property, promote soil microbial activities and quantity, strengthen sulfanilamide (SN)
Class antibiotic removes；There is very strong compatibility to microorganism；In soil can natural degradation, with after process simple and environmentally-friendly, no
Secondary pollution can be caused；And it is cheap.
Bacterium bacterial strain is (hereinafter referred to as enterobacter cloacae (Enterobacter cloacae strain) in the present invention
HM1) by being collected in the pig manure chosen property enrichment culture on Guangzhou breeding pig farm, isolate and purify and obtain.HM1 is to be disliked with sulfalene
The sulfamethoxazole degradation bacteria that azoles is grown for sole carbon source.The feces of livestock and poultry that other were fed sulfamethoxazole can also
Collection enterobacter cloacae.
The step that enterobacter cloacae is fixed on bagasse the present invention is：
Weigh bagasse, clean drying adds in conical flask after shredding, and is separately added into aseptic inorganic salt liquid after sterilization treatment
Body nutrient solution and enterobacter cloacae bacteria suspension, in same conical flask, mix sealing after 28-30 DEG C, and rotating speed is shaking of 150rpm
In bed, shaken cultivation to microbes biomass maximum obtains final product bagasse immobilization bacterium (HM1).
It is maximum that general culture can reach microbes biomass for 2 days.
Described bacterium bacterial suspension inoculation is in the inorganic salt liquid nutrient solution immersed with bagasse carrier, in 28 DEG C, rotating speed
In the shaking table of 150rpm, shaken cultivation is obtained immobilization bacterium (HM1) to biomass maximum.
Wherein bacterium bacteria suspension is prepared by following steps culture：
By microbionation after purification in 50mL fluid nutrient medium, and pH is adjusted to 7.0, in 28 DEG C, 150r/min's
On shaking table, shaken cultivation reaches maximum to the biomass of microorganism, the bacterium solution taking 4mL in centrifuge tube, under 4000r/min from
The heart is layered, and then pours out supernatant in centrifuge tube, supplements phosphate buffer and continues centrifugation layering, repeated centrifugation 3 times, makes thin
It is standby that bacterium bacteria suspension is placed in 4 DEG C of refrigerators.
When preparing bacteria suspension, general culture 36h can be maximum to microbes biomass, during centrifugation, is centrifuged 10min.
A kind of above-mentioned bagasse immobilization bacterium (HM1) answering in soil sulfa antibiotics sulfamethoxazole pollution amelioration
With.
Bagasse immobilization bacterium (HM1) of the present invention is applied in soil sulfa antibiotics sulfamethoxazole pollution amelioration
When, the consumption of bagasse immobilization bacterium is the contaminated soil being processed with bagasse carrier 1 weight portion in its 10 times of weight.
The present invention in terms of existing technologies, has advantages below：
Bagasse immobilization bacterium (HM1) used carrier biological material bagasse low cost of the present invention, bagasse immobilization bacterium
(HM1) simple and environmentally-friendly during the pollution of rehabilitating soil sulfa antibiotics sulfamethoxazole, do not result in secondary pollution, can improve simultaneously
The bioavailability of sulfa antibiotics, improves the efficiency of microbial degradation sulfa antibiotics.
The skill of biological material bagasse immobilization bacterium rehabilitating soil sulfa antibiotics sulfamethoxazole pollution of the present invention
Art, pollutes to eliminating sulfa antibiotics in soil, it is to avoid sulfa antibiotics pollution is good for the mankind by the transmission of food chain
The threat that health is constituted has highly important meaning.
【Brief description】
Fig. 1 is the effect of sulfamethoxazole in free bacteria (HM1) and bagasse immobilization bacterium (HM1) degraded soil；
Fig. 2 is the sterilizing and non-sterile soil impact to sulfamethoxazole in bagasse immobilization bacterium (HM1) degraded soil；
Fig. 3 attaches most importance to Metal Ions Cd2+And Pb2Shadow to sulfamethoxazole in bagasse immobilization bacterium (HM1) degraded soil
Ring；
Wherein, the transverse axis in Fig. 1-Fig. 3 be all the time (my god) axle, the longitudinal axis is all degradation rate (%) axle；
In Fig. 1, the meaning of each bar curve representative is：
■ control treatment (is not added with bacterium)
● free bacteria (HM1) processes (plus bacteria suspension 1mL)
▲ immobilization bacterium (HM1) processes (bagasse 1g+1mL HM1 bacteria suspension)
Immobilization bacterium (HM1) processes (bagasse 3g+1mL HM1 bacteria suspension)
◆ immobilization bacterium (HM1) processes (bagasse 6g+1mL HM1 bacteria suspension)
In Fig. 2, the meaning of each bar curve representative is：
■ non-sterile soil control treatment,
● the degradation rate of sulfamethoxazole in non-sterile soil,
▲ sterile soil control treatment,
The degradation rate of sulfamethoxazole in sterile soil.
In Fig. 3, the meaning of each bar curve representative is：
■ control treatment,
● plus bacterium process soil in sulfamethoxazole degradation rate,
—▲—Cd2+In the presence of plus bacterium process soil in sulfamethoxazole degradation rate,
—▼—Pb2+In the presence of plus bacterium process soil in sulfamethoxazole degradation rate.
【Specific embodiment】
The present invention a kind of bagasse immobilization bacterium (HM1), belongs to bacterium using bagasse for carrier immobilized enterobacter cloacae
(HM1), concrete preparation method is：
The pig manure on collection Guangzhou breeding pig farm, selective enrichment is cultivated, is isolated and purified out bacterium (HM1), carries out domestication training
Form bacterium bacteria suspension, then bacterial suspension inoculation is cultivated to microorganism on the bagasse carrier that inorganic salt liquid nutrient solution is processed
Biomass is maximum；
Wherein domestication culture is prepared the condition of bacteria suspension and is：PH is adjusted to 7.0, in 28 DEG C, the shaking table of 150r/min vibrates
Culture 36h is maximum to microbes biomass, and isolating bacteria suspension with the centrifugation of 4000r/min, to be placed in 4 DEG C of refrigerators standby；
The condition wherein preparing bagasse immobilization bacterium (HM1) is：Consumption by bacterium bacteria suspension and the bagasse processing
Ratio 1：After bagasse carrier, in 28 DEG C, shaking speed is to shake in 150rpm shaking table to 1～6 (ml/g) inoculated bacteria (HM1) bacteria suspension
Swing culture 2d, the biomass to microorganism reaches maximum, and presentation covers with bacterium for bagasse carrier.
Used by the present invention, various culture mediums are as follows：
Fluid nutrient medium：K2HPO40.5g, KH2PO40.5g, NaCl 0.2g, MgSO40.2g, NH4NO31.0g, sulphur
Amine first oxazole SMX 0.1g, trace element solution 10mL, distilled water l000mL, pH 7.5.Solid medium need to add 20-25g fine jade
Fat.
Minimal medium (inorganic salt liquid nutrient solution)：K2HPO40.5g, KH2PO40.5g, NaCl 0.2g, MgSO4
0.2g, NH4NO31.0g, trace element solution 10mL, distilled water l000mL, pH 7.2-7.5.
Trace element solution：FeSO40.1g/L, MnSO40.1g/L, ZnSO40.1g/L, Na2MoO40.01g/L,
CaCl20.1g/L, MgSO43g/L, CuSO40.1g/L.
It is selectively to be tamed and dociled with sulfamethoxazole for unique growth carbon source that the enterobacter cloacae of present invention collection belongs to bacterium (HM1)
Change culture and obtain.The HM1 so screening increases bacterial strain quantity by bagasse immobilization, to bacterium provide one good
Growth microenvironment is such that it is able to efficient degradation sulfamethoxazole.
With reference to specific embodiment, the present invention is described in further detail：
Embodiment 1：The effect of sulfamethoxazole in free bacteria (HM1) and bagasse immobilization bacterium (HM1) degraded soil
Relatively
Bacterial strain HM1 after will be purified is inoculated in 50mL fluid nutrient medium, and pH is adjusted to 7.0, in 28 DEG C, 150r/
Shaken cultivation 36h on the shaking table of min, the biomass to microorganism reaches maximum, the bacterium solution taking 4mL respectively in centrifuge tube, in
It is centrifuged 10min under 4000r/min, then supernatant in centrifuge tube is poured out, mend appropriate phosphate buffer and continue centrifugation
10min, is so repeated 3 times, and making bacteria suspension, to be placed in 4 DEG C of refrigerators standby.
Take from Agricultural University Of South China's arboretum for examination red earth, its quality is silt soil, pH value (water/soil=2.5/
1) be 5.35, organic is 7.89g/kg, nitrogen, phosphorus, potassium are other 0.71,0.72,2.61g/kg, sulfamethoxazole do not detect, mistake
2mm sieves.
Cross in 500g and in the soil of 2mm sieve, add the sulfamethoxazole acetone that 50mL sulfamethoxazole content is 2000mg/L
Solution, stirs, and the soil being crossed 2mm sieve after room-dry again with 500g is mixed, that is, manual simulation sulfalene is obtained and dislikes
Azoles contaminated soil.Camera bellows culture contaminated soil 4 weeks, in mensure contaminated soil before experiment, the initial concentration of sulfamethoxazole is
92.35mg/kg.
Prepared by bagasse immobilization bacterium (HM1)：Weigh the optimum carrier bagasse 1g having selected respectively, 3g, 6g clean and dry
Dry shred after be separately added in 250mL conical flask, every group parallel three, the 250mL conical flask simultaneously preparing sky is for use as free
The culture of microorganism, is placed in sterilizing 25min in the high-pressure sterilizing pot of 1.5Mpa together, shifts rapidly after pan gas drops subject to sterilization
To aseptic operating platform, sterilizing under uviol lamp is cooled to room temperature, then each in each conical flask adds aseptic inorganic of 5mL
Salt liquid medium, then it is separately added into 1mL bacterium (HM1) bacteria suspension in each conical flask with liquid-transfering gun, mix, air-tight bottle
Mouthful, labelled, it is placed in 28 DEG C, shaking speed is shaken cultivation in 150rpm shaking table, after 2d, add the pollution of 10g sulfamethoxazole
Soil carries out reparative experiment.
The following 3 kinds of process of test setting：Process 1：Control treatment (is not added with bacterium)；Process 2：Free bacteria (HM1) process (plus
Bacteria suspension 1mL)；Process 3-1：Immobilization bacterium (HM1) processes (bagasse 1g+1mL HM1 bacteria suspension)；Process 3-2：Fixation cell
Bacterium (HM1) processes (bagasse 3g+1mL HM1 bacteria suspension)；Process 3-3：Immobilization bacterium (HM1) processes (bagasse 6g+1mL HM1
Bacteria suspension)；
18 beakers are had under every kind of process, residual respectively at the 5th, 10,15,20,25 and 30d sampling and measuring sulfamethoxazoles
Allowance, every kind of process 3 is parallel.The moisture content keeping tested soil in each process in test is 15% about.After process terminates,
Measure and each process in contaminated soil the residual quantity of sulfamethoxazole and calculate the degradation rate of sulfamethoxazole, for equal sulfalene
The soil of oxazole level of pollution, bagasse immobilized bacterium (HM1) processes 3-1,3-2,3-3 and free bacterium (HM1) processes 2 repairing effects
As shown in Figure 1.
The degradation rate of sulfamethoxazole divides in control treatment, free bacteria process, immobilization bacterium are processed as seen from Figure 1
It is not：6.09%, 58.16%, 69.56%, 84.14%, 83.76%.Immobilization bacterium processes the fall making sulfamethoxazole
Solution rate specific ionization bacterial treatment has exceeded nearly 26 percentage points.This explanation immobilization bacterium is big to the degradation effect of sulfamethoxazole
It is better than greatly free bacteria.Simultaneously it can also be seen that, when bagasse consumption increases, in soil, the degradation rate of sulfamethoxazole also has increasing
Plus, when bagasse consumption increases to 6g, degradation rate increase is less obvious, and this is due to when bagasse consumption increases to 3g, micro-
Carbon source needed for biological growth is relatively sufficient, therefore in actual application, is cost-effective, processes the bacteria residue ratio of 3-2
For optimal proportion.
Embodiment 2：The sterilizing and non-sterile soil shadow to sulfamethoxazole in bagasse immobilization bacterium (HM1) degraded soil
Ring
With embodiment 1, bacterium added by each process is bagasse immobilization bacterium (HM1) to bacteria suspension, solid for examination soil and bagasse
Surely change bacterium (HM1) preparation with embodiment 1, measuring the sulfamethoxazole initial concentration in contaminated soil before experiment is 92.35mg/
kg.
Following 4 process of the present embodiment setting：
Process 1：Non- sterilized soil adds bacterium and processes (plus bagasse immobilization HM1 (1ml：3g))
Process 2：Non- sterilized soil control treatment (being not added with bacterium)
Process 3：Sterilized soil adds bacterium and processes (plus bagasse immobilization HM1 (1ml：3g))
Process 4：Sterilized soil control treatment (is not added with bacterium)
18 beakers are had under every kind of process, residual respectively at the 5th, 10,15,20,25 and 30d sampling and measuring sulfamethoxazoles
Allowance, every kind of process 3 is parallel.The moisture content keeping tested soil in each process in test is 15% about.
After process terminates, measure and each process in contaminated soil the residual quantity of sulfamethoxazole and calculate the fall of sulfamethoxazole
Solution rate, for the soil of equal sulfamethoxazole level of pollution, in sterilizing and non-sterile soil, the degradation rate of sulfamethoxazole is such as
Shown in Fig. 2, no matter whether soil disinfection adds bacterium before processing 10d, and the degradation rate of sulfamethoxazole increases sharply, sulfalene after 10d
The degradation rate of oxazole slowly increases, and degradation rate change after 20d tends towards stability.Bagasse immobilization bacterium (HM1) is added to process 30d
Afterwards, in non-sterilized soil, the degradation rate of sulfamethoxazole reaches 84.14%, higher than the degradation rate of sulfamethoxazole in sterilized soil
79.09%, no matter whether soil sterilizes, the degradation rate difference very little of sulfamethoxazole in soil, therefore, sterilizing and non-sterilizing are right
In soil, the degradation rate impact of sulfamethoxazole is less.
Embodiment 3：Heavy metal in soil ion Cd2+And Pb2To sulfalene in bagasse immobilization bacterium (HM1) degraded soil
The impact of oxazole
With embodiment 1, bacterium added by each process is bagasse immobilization bacterium (HM1) to bacteria suspension, solid for examination soil and bagasse
Surely change bacterium (HM1) preparation with embodiment 1, but in sterilizing inorganic salt liquid culture medium therein, contain 0.01% PbC1 respectively2
And CdC12, the initial concentration measuring sulfamethoxazole in contaminated soil before experiment is 92.35mg/kg.
Following 4 process of the present embodiment setting：
Process 1：Control treatment (is not added with bacterium)
Process 2：Plus bacterium process (plus bagasse immobilization HM1 (1ml：3g))
Process 3：Cd2++ plus bacterium process (Cd2++ bagasse immobilization HM1 (1ml：3g))
Process 4：Pb2++ plus bacterium process (Pb2++ bagasse immobilization HM1 (1ml：3g))
18 beakers are had under every kind of process, residual respectively at the 5th, 10,15,20,25 and 30d sampling and measuring sulfamethoxazoles
Allowance, every kind of process 3 is parallel, and the moisture content keeping tested soil in each process in test is 15% about.
After process terminates, measure and each process in contaminated soil the residual quantity of sulfamethoxazole and calculate the fall of sulfamethoxazole
Solution rate.For the soil of equal sulfamethoxazole level of pollution, in the presence of different heavy metal ion, sulfamethoxazole in soil
Degradation rate change is as shown in figure 3, heavy metal in soil ion Cd2+And Pb2Presence to bagasse immobilization bacterium (HM1) repair sulphur
Amine first oxazole contaminated soil all has a certain impact, and after processing 30d, heavy metal free ion adds bacterium process, Cd2++ plus bacterium process
And Pb2++ plus bacterium process degradation rate respectively 84.14%, 80.93% and 80.61%, Cd2+In the presence of sulfamethoxazole in soil
Degradation rate more no Cd2+In the presence of degradation rate reduce only 4%, even if there is heavy metal ion Cd in soil2+And Pb2+, sugarcane
Slag immobilization bacterium (HM1) still can reach more than 80% to the degradation rate of sulfamethoxazole in soil.
The conclusion of above three embodiment is summarized as:
1st, for sulfamethoxazole initial concentration be 92.35mg/kg soil, bagasse immobilization bacterium (HM1) and dissociate
After bacterium (HM1) processes 30d, the degradation rate of sulfamethoxazole is respectively 84.14% and 58.16%, shows bagasse fixation cell
Bacterium (HM1) is significantly better than free bacterium to the degradation effect of sulfamethoxazole.
2nd, after bagasse immobilization bacterium (HM1) processes 30d, in non-sterilized soil, the degradation rate of sulfamethoxazole reaches
84.14%, and in sterilized soil, the degradation rate of sulfamethoxazole is 79.09%, no matter whether soil sterilizes, in soil, sulfalene is disliked
The degradation rate gap of azoles is less, and therefore, edaphon is to sulfamethoxazole in bagasse immobilization bacterium (HM1) degraded soil
Impact is less.
3rd, heavy metal ion Cd in soil2+And Pb2+Bagasse immobilization bacterium (HM1) is processed with sulfalene in soil dislike
Azoles all has certain impact, even if but there is heavy metal ion Cd in soil2+And Pb2+, bagasse immobilization bacterium is to sulfanilamide (SN) in soil
The degradation rate of first oxazole still can reach more than 80%.These explanation bagasse immobilizations do not only have sulfalene in stronger degraded soil
The ability of oxazole, and have preferable adaptive capacity to environment.
It can be seen that, bagasse immobilization bacterium (HM1) of the present invention has preferable adaptive capacity to environment, for it in complicated soil ring
Application in border provides guarantee.
Claims (10)
1. bagasse immobilization bacterium is it is characterised in that be carrier immobilized enterobacter cloacae Pseudomonas using bagasse.
2. bagasse immobilization bacterium according to claim 1 is it is characterised in that described bagasse is trained through inorganic salt liquid
Nutrient solution is processed.
3. bagasse immobilization bacterium according to claim 1 is it is characterised in that described enterobacter cloacae is being inoculated in sugarcane
It is prepared into bacteria suspension respectively through culture in advance during slag carrier.
4. bagasse immobilization bacterium according to claim 3 is it is characterised in that described bacteria suspension and the bagasse processing
Amount ratio be 1mL：1～6g.
5. bagasse immobilization bacterium according to claim 4 is it is characterised in that be fixed on enterobacter cloacae on bagasse
Step is：
Weigh bagasse, clean drying adds in conical flask after shredding, and adds aseptic inorganic salt liquid nutrient solution after sterilization treatment,
Add the bacteria suspension of enterobacter cloacae, mix sealing after 28 DEG C, rotating speed is shaken cultivation extremely micro- life in the shaking table of 150rpm
Thing biomass maximum obtains final product bagasse immobilization bacterium.
6. bagasse immobilization bacterium according to claim 5 is it is characterised in that described bacteria suspension is trained by following steps
Support and be prepared：
Bacterium after purification is inoculated in 50mL fluid nutrient medium respectively, and pH is adjusted to 7.0, in 28 DEG C, 150r/min's
On shaking table, shaken cultivation reaches maximum to the biomass of microorganism, the bacterium solution taking 4mL in centrifuge tube, under 4000r/min from
The heart is layered, and then pours out supernatant in centrifuge tube, supplements phosphate buffer and continues centrifugation layering, repeated centrifugation 3 times, makes thin
It is standby that bacterium bacteria suspension is placed in 4 DEG C of refrigerators.
7. bagasse immobilization bacterium according to claim 5 is it is characterised in that described inorganic salt liquid nutrient solution and sugarcane
The amount ratio of slag is 10mL：3g.
8. bagasse immobilization bacterium according to claim 5 is it is characterised in that described enterobacter cloacae is wide by being collected in
The pig manure chosen property enrichment culture on state breeding pig farm, isolate and purify and obtain.
9. the bagasse immobilization bacterium any one of a kind of claim 1-8 is in soil sulfamethoxazole pollution amelioration
Application.
10. application in soil sulfamethoxazole pollution amelioration for a kind of bagasse immobilization bacterium according to claim 9,
It is characterized in that the consumption of described bagasse immobilization bacterium is the Polluted Soil being processed with bagasse carrier 1 weight portion in its 10 times of weight
Earth.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108823115A (en) * | 2018-05-15 | 2018-11-16 | 浙江省农业科学院 | A kind of sulfa antibiotics degradation Alcaligenes and its application |
| CN108977431A (en) * | 2018-06-25 | 2018-12-11 | 华南农业大学 | Bagasse immobilization microorganism Aspergillus aculeatus and its application in quinolone antibiotics pollution amelioration |
| CN109127690A (en) * | 2018-08-31 | 2019-01-04 | 陈毅忠 | A kind of preparation method of soil remediation material |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102373191A (en) * | 2011-09-26 | 2012-03-14 | 华南理工大学 | Dominant consortium flora for degrading urban waste water and preparation method thereof |
| CN104388096A (en) * | 2014-09-25 | 2015-03-04 | 广西大学 | Bagasse composite soil conditioner preparation method |
| CN104762288A (en) * | 2015-04-08 | 2015-07-08 | 华南农业大学 | Bagasse immobilized combination bacterium and application thereof in soil polyaromatic hydrocarbon phenanthrene pollution restoration |
-
2016
- 2016-10-19 CN CN201610912546.3A patent/CN106434614A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102373191A (en) * | 2011-09-26 | 2012-03-14 | 华南理工大学 | Dominant consortium flora for degrading urban waste water and preparation method thereof |
| CN104388096A (en) * | 2014-09-25 | 2015-03-04 | 广西大学 | Bagasse composite soil conditioner preparation method |
| CN104762288A (en) * | 2015-04-08 | 2015-07-08 | 华南农业大学 | Bagasse immobilized combination bacterium and application thereof in soil polyaromatic hydrocarbon phenanthrene pollution restoration |
Non-Patent Citations (3)
| Title |
|---|
| G. YASODARA LIYANAGE ET AL.: "Evaluation of Amoxicillin and Sulfonamide Removal by Bacillus cereus，Enterobacter ludwigii and Enterobacter sp.", 《ENVIRONMENT AND NATURAL RESOURCES J.》 * |
| 李明亮: "环境中磺胺类药物的降解行为及影响因素的研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 林抗美 等: "有机磷农药降解菌-阴沟肠杆菌的生物学特性", 《中国农学通报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108823115A (en) * | 2018-05-15 | 2018-11-16 | 浙江省农业科学院 | A kind of sulfa antibiotics degradation Alcaligenes and its application |
| CN108823115B (en) * | 2018-05-15 | 2020-06-23 | 浙江省农业科学院 | Sulfonamide antibiotic degradation alcaligenes and application thereof |
| CN108977431A (en) * | 2018-06-25 | 2018-12-11 | 华南农业大学 | Bagasse immobilization microorganism Aspergillus aculeatus and its application in quinolone antibiotics pollution amelioration |
| CN109127690A (en) * | 2018-08-31 | 2019-01-04 | 陈毅忠 | A kind of preparation method of soil remediation material |
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