CN106434458B - A kind of Paenibacillus polymyxa and its microbial inoculum and preparation and application - Google Patents
A kind of Paenibacillus polymyxa and its microbial inoculum and preparation and application Download PDFInfo
- Publication number
- CN106434458B CN106434458B CN201610887157.XA CN201610887157A CN106434458B CN 106434458 B CN106434458 B CN 106434458B CN 201610887157 A CN201610887157 A CN 201610887157A CN 106434458 B CN106434458 B CN 106434458B
- Authority
- CN
- China
- Prior art keywords
- paenibacillus polymyxa
- parts
- bacterial agent
- microbial bacterial
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The present invention relates to a kind of Paenibacillus polymyxa bacterial strain Paenibacillus polymyxa DP-11 and its microbial inoculum and preparation and application.Paenibacillus polymyxa Paenibacillus polymyxa DP-11 provided by the invention, it is preserved in China General Microbiological culture presevation administrative center, deposit number is CGMCC NO.5214, and microbial bacterial agent provided by the invention, active constituent is Paenibacillus polymyxa DP-11.Using bacterial strain provided by the invention and microbial bacterial agent, crops can be significantly increased for the disease resistance of wilt disease, improve crops quality, especially improvement balsam pear is for the disease resistance of wilt disease, and effect of increasing production is obvious, amount of increase in production 11.11%-17.59%.
Description
Technical field
The present invention relates to a kind of Paenibacillus polymyxas, and in particular to a kind of Paenibacillus polymyxa (Paenibacillus
) and microbial bacterial agent and the Paenibacillus polymyxa and bacterium using Paenibacillus polymyxa production polymyxa
Application of the agent in prevention and treatment crops wilt disease and/or plant growth-promoting field.
Background technique
Crops wilt disease is a kind of worldwide soil-borne disease, mainly by Fusarium oxysporum (Fusarium oxysporum)
It infects and causes.Wherein, the most serious with the harm of vegetables cucurbits fusarium wilt, and once fall ill, it is difficult to it eradicates, as the continuous cropping time increases
Add, wilt disease is on the rise.In recent years, with the development of China's industrialized agriculture, facilities vegetable melon crop area is continuously increased,
In addition long-term continuous cropping, pathogen accumulation is continuously increased in soil, and vegetables cucurbits fusarium wilt occurs more and more common, is endangered more next
It is heavier.General diseased plant rate 10%~20%, serious plot is up to 50%~80%, or even can cause to have no harvest, it has also become hinders China
An important factor for vegetables melon production development.
Currently, the control measure of vegetables cucurbits fusarium wilt is mainly the breeding and chemical prevention of disease-resistant variety.Disease-resistant variety
Breeding and utilization are the most economical effectively preventing measures of crops wilt disease prevention and treatment, but since the disease-resistant variety breeding period is long, and
The transformant numerous types of wilt disease cause of disease, Physiological Race Differentiation are frequent, there is no the disease-resistant variety of efficient stable in production so far
It is widely applied；Chemical prevention is the upper most common means of prevention of current production, but since chemical prevention is easy to produce environment
Productions and the food-safety problem such as pollution, " 3R " and " 3 cause ", therefore it is applied on vegetable melon and fruit produces by certain limit
System.Biological control is because its environment compatibility is good, control efficiency well has become the effective way that wilt disease prevents and treats, still, mesh
Preceding Related product and technology in relation to biological control in actual production using still immature.
Balsam pear is important one of health-care vegetable, plants, has high economic benefit extensively in China, general mu income
Up to 6000~8000 yuan, ten thousand yuan or more are reached as high as.But with the continuous increase of cultivated area, and generally existing continuous cropping connects
Make phenomenon, bitter gourd wilt bacterium number amount accumulates year by year, and bitter gourd wilt evil occurs and harm is on the rise, additionally, due to chemical fertilizer
The unreasonable application of pesticide causes soil microenvironment to deteriorate, carbon in soil/nitrogen ratio imbalance, and agriculture chemical residual, disease pest are anti-
The problem of a series of puzzlement balsam pears such as pharmacological property produce, has seriously affected the economic benefit and social benefit of balsam pear plantation.Balsam pear is withered
Disease of withering is typical plant soil-borne diseases, and soil microenvironment and weather conditions are to influence balsam pear normal growth and wilt disease hair
Raw important ecological factor, suitable soil microenvironment and weather conditions are also balsam pear good quality and high output and show liquor style
The deciding factor of characteristic.But weather conditions have uncertain and uncontrollability, and only adaptability utilizes it in production, because
This, adjusting and utilization to soil micro-ecosystem become the Critical policies for bitter gourd wilt prevention and control, are especially lacking stable height
In the balsam pear production of anti-kind, it can ensure that the healthy growth of balsam pear and effectively control are withered by adjusting soil micro-ecosystem function
The occurring and damage of disease.Research and development centered on healthy growth of crops, by soil micro-ecosystem regulate and control based on, with anti-disease tolerant variety breeding
For guarantee can effectively prevention and control continuous croppings bitter gourd wilt harm comprehensive treatment key technology, and be widely applied, can
It really realizes that fertilizer and pesticide is reduced the modern agricultural development requirement used, to raising quantity production of bitter melon, ensures balsam pear quality and maintenance
The sustainable development of balsam pear planting industry is of great significance.
Summary of the invention
Problem of the prior art solved by the invention is: existing biocontrol bacterial strain activity is not high, and control efficiency is unstable,
And due to the continuous variation of soil fertility condition, nutrient and the content of organic matter, new micro- life more adaptable is needed
Object bacterial strain.
Specifically, the present invention provides the following technical scheme that
On the one hand, the present invention provides a kind of Paenibacillus polymyxa Paenibacillus polymyxa DP-11, protect
It is hidden in China General Microbiological culture presevation administrative center, deposit number is CGMCC NO.5214.
Second aspect, the present invention also provides a kind of microbial bacterial agents, glue class gemma using containing above-described more
Bacillus Paenibacillus polymyxa DP-11 produces to obtain, and active constituent is above-described Paenibacillus polymyxa
Paenibacillus polymyxa DP-11。
Preferably, the microbial bacterial agent further includes diatomite, SDS and sodium lignin sulfonate.
Preferably, by weight percentage, the microbial bacterial agent includes described diatomite 10-12%, SDS1.2-
2.4%, sodium lignin sulfonate 2-4% and Paenibacillus polymyxa Paenibacillus polymyxa DP-11 84.6-
87.8%, the sum of each component content is 100%.
The third aspect, the present invention also provides above-described Paenibacillus polymyxa Paenibacillus polymyxa
The application of DP-11 or microbial bacterial agent described above in prevention and treatment bitter gourd wilt and/balsam pear growth-promoting field.
Preferably, the Paenibacillus polymyxa Paenibacillus polymyxa DP-11 or the microbial bacterial agent
In active constituent DP-11 be 20,000,000,000-400 hundred million CFU/g.
Fourth aspect, the present invention also provides a kind of prevention and treatments to wither the method for disease as balsam pear, will it is above-described more glue class buds
Microbial bacterial agent described in spore bacillus Paenibacillus polymyxa DP-11 or any of the above item is added in soil.
5th aspect, the present invention also provides the preparation methods of microbial bacterial agent described in any of the above item, pass through packet
The preparation method for including following steps obtains:
(1) first order seed culture: by above-described Paenibacillus polymyxa Paenibacillus polymyxa DP-
11 are inoculated in culture medium and cultivate, and obtain primary seed solution；
(2) secondary seed culture: the primary seed solution that step (1) obtains is connect according to the inoculum concentration of 3%-5% volume ratio
Enter in broth bouillon and cultivate, obtains secondary seed solution；
(3) fermented and cultured: the secondary seed solution that step (2) obtains is inoculated according to the inoculum concentration of 5%-20% volume ratio
It is cultivated in solid fermentation culture medium.
Preferably, the temperature of first order seed culture is 30-35 DEG C in step (1), incubation time 20-30h.
Preferably, the temperature of step (2) second level culture is 30-35 DEG C, incubation time 2-4d.
Preferably, incubation time is 72-144h in step (3), and solid fermentation culture medium includes: sugar grass in parts by weight
15-25 parts of straw powder, 20-30 parts of wheat bran, 5-15 parts of rice bran, 15-25 parts of bean cake powder, 10-20 parts of starch, 3-8 parts of medical stone, ferment
0.5-2 parts of female powder, 2-4 parts of calcium carbonate, 0.3-0.8 parts of magnesium sulfate, 0.5-2 parts of potassium dihydrogen phosphate, 0.3-0.8 parts of manganese sulfate.
Paenibacillus polymyxa provided in the present invention is Paenibacillus polymyxa DP-11, is from Chinese river
It separates, protects in the rhizosphere soil of the Xinyang Nan Sheng Mt Jigong gold inlaid jade bamboo (Phyllostachys heteroclada)
It is hidden in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), address are as follows: the Chaoyang District, Beijing City North Star
No. 3 Institute of Microorganism, Academia Sinica, institute of West Road 1, postcode: 100101, deposit number is CGMCC No.5214, is protected
Hiding the date is on 09 05th, 2011.
Beneficial effect obtained by the present invention is: utilizing bacterial strain provided by the invention and microbial bacterial agent, can significantly increase
Strong crops improve crops quality, especially improvement balsam pear for the disease resistance of wilt disease, increase for the disease resistance of wilt disease
It is obvious to produce effect, amount of increase in production 11.11%-17.59%.
Below with reference to each specific embodiment, the present invention and its advantageous effects are described in detail.
Specific embodiment
As described above, the present invention provides a kind of Paenibacillus polymyxa and its microbial inoculum and preparation and application.
Wherein, the formula of the culture medium used in the present invention is as follows:
Nutrient agar (NA): tryptone 10g, beef extract 3g, sodium chloride 5g, water 1L, agar 15g, 25 DEG C, pH 7.2~
7.5。
Nutrient broth (NB): tryptone 10g, beef extract 3g, sodium chloride 5g, water 1L, 25 DEG C, pH 7.2~7.5.
Solid fermentation culture medium: in parts by weight, 20 parts of sweet sorghum stalk powder, 25 parts of wheat bran, 10 parts of rice bran, bean cake powder 20
Part, 15 parts of starch, 5 parts of medical stone, 1 part of yeast powder, 3 parts of calcium carbonate, 0.5 part of magnesium sulfate, 1 part of potassium dihydrogen phosphate, manganese sulfate 0.
Part；Material-water ratio 1:1.1 (W/V, g/L), pH 9.0~10.0, it is spare after 0.15MPa high pressure sterilization 60min.
Wherein, the raw material used in the present invention and instrument are raw material and instrument commonly used in the art.Sweet sorghum stalk powder,
Wheat bran, rice bran, bean cake powder etc. are purchased from market.Specifically, the model of raw materials and reagents used in embodiment and factory
Family is shown in Table 1.
1 the raw materials used in the present invention of table and instrument and producer
Embodiment one
Paenibacillus polymyxa Paenibacillus polymyxa DP-11 provided by the invention is gram-positive bacteria,
Rod-shaped, endospore generates bacterium, and the best growing condition of bacterial strain is 30-32 DEG C, pH value 7.2, and passes through morphological classification
Method and 16S rRNA gene sequencing and biological organism automatic analysis system are accredited as Paenibacillus polymyxa
(Paenibacillus polymyxa).The 16S rRNA sequence of the bacterial strain has been filed on GenBank database, and accession number is
NJ570759。
By the above qualification result, confirmation bacterial strain DP-11 is Paenibacillus polymyxa bacterial strain, is named as more viscous class buds
Spore bacillus strain DP-11 (Paenibacillus polymyxa DP-11), is preserved in China General Microbiological culture presevation pipe
Reason center.
Embodiment two
Microbial bacterial agent is produced using above-mentioned Paenibacillus polymyxa DP-11
(1) preparation of seed fermentation liquid
In the Paenibacillus polymyxa DP-11 bacterial strain access NA fluid nutrient medium that inclined-plane is saved, in 30
DEG C, for 24 hours, primary seed solution is made in shaken cultivation under 170r/min.
Then primary seed solution is seeded in NB culture medium in 30 DEG C, 170r/min item with the inoculum concentration of 3% volume ratio
Shaken cultivation 3d makes secondary seed solution under part.
Wherein, the assay of effective viable bacteria uses dilution-plate method in secondary seed solution, and calculation formula is as follows:
Viable bacteria content (cfu/mL)=each flat-plate bacterial colony number × extension rate dilution/coating bacterium solution volume (mL)
The effective viable bacteria content of the secondary seed solution for measuring DP-11 according to formula reaches 108Cfu/ml or more could conduct
The Inoculant of solid fermentation.
(2) solid fermentation
Liquid two stage seed is inoculated in solid fermentation training respectively with the inoculum concentration of 5%, 10%, 15% and 20% (V/V)
It supports and is mixed well in base, divided in the tray of sterilizing, be placed on fermenting frame with the thickness of 6cm, 30 DEG C~32 DEG C conditions of room temperature
Lower fermentation 72h, microscopy sporulation rate obtain DP-11 solid fermentation object.
Wherein, the results are shown in Table 2 for the solid fermentation of active bacterial strain DP-11.
Table 2 the result shows that, in the composite test of 3 fermentation times, bacterial strain DP-11 secondary seed solution inoculum concentration be 15%
When, sporulation rate is all remarkably higher than the combination of other inoculum concentrations, wherein inoculum concentration 15%, when fermentation time is 72h,
Sporulation rate is 97.23%, is significantly higher than other combinations, this research selects inoculum concentration 15%, and fermentation time 72h is bacterial strain
The solid fermentation condition of DP-11.
The influence of 2 different vaccination amount of table and fermentation time to DP-11 solid fermentation effect
Note: indicating significant difference (p≤0.05) with capitalizations different after column of figure, wherein the difference represented from A to D
Conspicuousness successively increases.
Embodiment three
The microbial bacterial agent obtained using embodiment two screens other carriers and surface-active as active constituent
Agent is prepared wettable microorganism powder microbial inoculum (as wettable bacterium powder).
Respectively using white carbon black, kaolin, diatomite, bentonite, talcum powder as carrier filler, with SDS, lignin sulfonic acid
Sodium, polysorbate60 and D425 are that surfactant is fitted into mixing and blending machine with DP-11 solid fermentation object according to a certain percentage, are filled
Divide mixing, measures MEBO ribbon gauze, suspensibility and spore content and adsorption capacity.
By measurement different carriers filler and surfactant to the Different Effects of DP-11 solid fermentation object, filter out
Optimum carrier filler and most suitable surfactant, then by it according to optimal proportion, the carrier that will be filtered out
Filler and surfactant are mixed with DP-11 solid fermentation object, are chemically examined, packaging, as wettable bacterium powder.
Wherein, different preparations is measured referring to " National Standard of the People's Republic of China ".Wetting time measurement ginseng
According to GB/T 5451-2001, referring to GB/T 14825-2006, moisture is surveyed referring to GB/T 1600-2001, storage for suspensibility measurement
Experiment is carried out referring to the method for GB/T19136-2003.Using the method for plate culture count, spore content is measured.
(1) screening of filler
5 kinds of different carriers such as white carbon black, kaolin, diatomite, bentonite, talcum powder are selected to do filler respectively, wherein white
Carbon black, kaolin, diatomite, bentonite, talcum powder and DP-11 solid fermentation object weight ratio be 10%, it is more different to fill out
Expect the influence to wettable bacterium powder physicochemical property.Comprehensively consider the factors such as different wetting time, adsorption capacity and price, determines system
Make the most suitable filler of wettable bacterium powder.Wherein, measurement result is shown in Table 3.
(2) screening of surfactant
Added respectively in the solid fermentation object that embodiment two obtains a certain amount of SDS, sodium lignin sulfonate, polysorbate60 and
Different preparations is made in the auxiliary agents such as D425.Then the suspensibility of different preparations, wetting time and spore content are measured.Wherein,
Measurement result is shown in Table 4.
(3) original powder storage-stable measures
Weigh DP-11 wettable bacterium powder 50g, the i.e. same diatomite of DP-11 solid fermentation object, SDS and sodium lignin sulfonate
Mixture, wherein calculated in weight percent, the content of DP-11 is 85%, and the content of diatomite is the content of 10%, SDS
Content for 2%, wooden disulfonate acid is 3%, is placed in (4 ± 2) DEG C and stores under room temperature, every 30d measurement sample
Number of viable, measure duration 360d, specify wettable bacterium powder storage 360d during number of viable variation.
Wherein measurement result is as follows:
(1) the selection result of filler
Different fillers has a certain impact to the physicochemical property tool of wettable bacterium powder.For solid bacterium powder, selection absorption
The suitable filler of capacity.Table 3 the result shows that, secondly it is diatomite that white carbon black adsorption capacity is maximum and mobility is maximum, but
It is after DP-11 mixes storage 30d at room temperature with white carbon black, number of viable is decreased obviously in wettable bacterium powder, shows white carbon black
It is poor with the biocompatibility of bacterial strain DP-11, and white carbon black is on the high side, therefore this research selects diatomite as filler.
Influence of 3 filler of table to preparation performance
(2) the selection result of surfactant
Since most of dispersing agent (surfactant) has one with a degree of wetting action, most of wetting agent
Determine the peptizaiton of degree, therefore the suspension effect of this experiment Surfactant and wetting action are investigated simultaneously.As a result such as table 4
It is shown.Using surfactant SDS as dispersing agent, the suspensibility highest of preparation reaches 84%, but its wetting time is compared with other materials
It is long, it is 21s, wetting agent is made with sodium lignin sulfonate, the wet performance of preparation is optimal, but its suspensibility is low compared with SDS, the bud of the two
Born of the same parents' content is apparently higher than other reagents, therefore SDS is selected to compound the dispersing agent as this research with sodium lignin sulfonate.
Influence of the 4 different surfaces activating agent of table to preparation performance
| Surfactant | Suspensibility (%) | Wetting time (s) | Brood cell's content (× 109cfu/g) |
| SDS | 84 | 21 | 4.85 |
| Sodium lignin sulfonate | 76 | 10 | 4.36 |
| Polysorbate60 | 34 | 17 | 3.91 |
| D425 | 74 | 18 | 3.14 |
(3) DP-11 wettable bacterium powder storage-stable measurement result
Table 5 the result shows that, under the conditions of room temperature storage, number of viable in DP-11 wettable bacterium powder is significantly lower than refrigeration
Condition.After storing 360d under room temperature environment, number of viable is the 59.6% of original powder, and is protected at refrigerated condition (4 ± 2) DEG C
360d is deposited, number of viable is the 80.6% of original powder, has dropped 40.4% and 19.4% respectively.Analyze its reason may be due to
Under low temperature, in the form of brood cell in a dormant state, physiological metabolism is slow for thallus.Therefore, DP-11 wettable bacterium powder should be in low temperature
Under the conditions of save and be advisable.
Viable count in DP-11 wettable bacterium powder in the different storage number of days of table 5
(4) brood cell's assay result of active bacterial strain DP-11 original powder
It is compounded using diatomite as carrier, with SDS with sodium lignin sulfonate as dispersing agent, is mixed into active bacterial strain DP-11 respectively
After bacterium powder, wherein by weight, the content of DP-11 is 85%, the content of diatomite is 10%, the content of SDS is 2%, wood
The content of matter disulfonate acid is 3%, after being mixed, drying, obtain DP-11 wettable bacterium powder.Testing result shows
Viable bacteria content is 20,000,000,000-400 hundred million cfu/g in DP-11 Inoculant.
Example IV
Wettable bacterium powder (wettable Inoculant) prepared by embodiment three is mixed with base fertilizer, for verifying for hardship
The control efficiency of cucurbit wilt.Wherein, wettable bacterium powder by weight percentage, including Paenibacillus polymyxa
DP-11 85%, diatomite 10%, SDS 2% and sodium lignin sulfonate 3%.
Wettable Inoculant is mixed into base manure according to the ratio that mass ratio is 1:200, after cave is applied or Gansu Province applies, Shi Shaoliang water
The solid root of ridging.Wherein, base manure is Xi Mandi board compound fertilizer, and additive amount is 50 kgs/acre.It can effectively prevent in balsam pear growth morning
The generation of phase wilt disease.Wherein, the testing result for different batches in Nanning is shown in Table 6- table 9.Processing group and blank group
Three groups of repeating groups are respectively set, wherein blank group does not add wettable bacterium powder.The result of table 6 and table 7 is using at the above formula
The comparison result of the disease incidence of reason group and blank group bitter gourd wilt.
The generation of middle and later periods wilt disease in order to prevent, can be in balsam pear flowering stage, by wettable Inoculant according to quality volume
Inoculant is dissolved in irrigating through furrow in liquid fertilizer by the ratio than 1:200 to be used.Wherein, liquid fertilizer uses the " knob of Ge Linmei company
It is emerald green " rich carbon humic acid imitates Liquid Fertilizer entirely.There is antagonistic activity to bitter gourd wilt bacterium, and it is micro- with good growth-promoting functions
The microbial inoculant wettable powder that active bacterial strain spore content is greater than 20,000,000,000/gram is made in biological bacterial strain.The result of table 8
For using the comparison result for the disease incidence for being formulated obtained processing group and blank group bitter gourd wilt above, table 9 is more than
The comparison of different batches and Different treatments (i.e. solid base manure and liquid top dressing) processing group and blank group balsam pear average product
As a result.
Note: when cave is applied, the depth in cave should be appropriate, unsuitable too deep, is advisable with 5~8cm；When field planting, reduce to the greatest extent
Injury to planting stock root system.
The disease incidence of 6 processing group of table and blank group bitter gourd wilt compares (2016.4.27, Nanning)
The disease incidence of 7 processing group of table and blank group bitter gourd wilt compares (2016.5.9, Nanning)
The disease incidence of 8 processing group of table and blank group bitter gourd wilt compares (2016.5.23, Nanning)
9 processing group of table and blank group balsam pear average product compare (2016.4.27~2016.5.23, Nanning)
It can be seen that the balsam pear handled using wettable bacterium powder of the invention, the morbidity of wilt disease from the result of table 6- table 9
Rate highest reduces 18.28%, and yield highest increases 17.59%.In conclusion using biocontrol bacteria DP-11 as active bacterial strain,
Diatomite is carrier, SDS is compounded with sodium lignin sulfonate is dispersing agent, uses solid fermentation that viable bacteria content is made as 20,000,000,000
The wettable Inoculant of cfu/g.The Inoculant can significantly increase balsam pear to the disease resistance of wilt disease, improve balsam pear quality, volume increase
Effect is obvious, and the average control efficiency of bitter gourd wilt is 78.63%, and effect of increasing production is 11.11%~17.59%.
Meanwhile on the basis of the above formula, by adjusting the weight percent of each ingredient of microbial bacterial agent, by diatomite
Mass fraction control in microbial bacterial agent controls mass fraction of the SDS in microbial bacterial agent in 1.2- in 10-12%
2.4%, by mass fraction control of the sodium lignin sulfonate in microbial bacterial agent in 2-4% and by Paenibacillus polymyxa
The mass fraction of Paenibacillus polymyxa DP-11 solid fermentation object is controlled in 84.6-87.8%, can be played same
The same or similar technical effect of example IV.Bacterial strain and microbial bacterial agent provided by the present invention can be used for preventing and treating crop
Wilt disease
The foregoing is merely present pre-ferred embodiments, are not used to the limitation present invention, all in spirit and original of the invention
The modifications, equivalent substitutions and improvements etc. done within then are required within the protection scope of invention.
Claims (9)
1. a kind of Paenibacillus polymyxa Paenibacillus polymyxa DP-11, is preserved in China General Microbiological strain
Preservation administrative center, deposit number are CGMCC NO.5214.
2. a kind of microbial bacterial agent, which is characterized in that including Paenibacillus polymyxa according to claim 1
Paenibacillus polymyxa DP-11, diatomite, SDS and sodium lignin sulfonate, the Paenibacillus polymyxa
Paenibacillus polymyxa DP-11 is the active constituent of microbial bacterial agent.
3. microbial bacterial agent according to claim 2, which is characterized in that by weight percentage, the microbial bacterial agent
Including the diatomite 10-12%, SDS 1.2-2.4%, sodium lignin sulfonate 2-4% and Paenibacillus polymyxa
Paenibacillus polymyxa DP-11 84.6-87.8%, the sum of each component content are 100%.
4. according to any microbial bacterial agent of claim 2-3, which is characterized in that the Paenibacillus polymyxa
Paenibacillus polymyxa DP-11 is 20,000,000,000-400 hundred million CFU/g.
5. a kind of prevention and treatment bitter gourd wilt and the method for promoting balsam pear production, which is characterized in that will be appointed according to claim 2-4
Microbial bacterial agent described in one is added in soil.
6. according to the preparation method of any microbial bacterial agent of claim 2-3, which is characterized in that it is by including as follows
The preparation method of step obtains:
(1) first order seed culture: by Paenibacillus polymyxa Paenibacillus polymyxa DP- described in claim 1
11 are inoculated in culture medium and cultivate, and obtain primary seed solution；
(2) secondary seed culture: the primary seed solution that step (1) is obtained accesses meat according to the inoculum concentration of 3%-5% volume ratio
It is cultivated in soup culture medium, obtains secondary seed solution；
(3) secondary seed solution that step (2) obtains fermented and cultured: is inoculated in solid according to the inoculum concentration of 5%-20% volume ratio
It is cultivated in fermentation medium.
7. preparation method according to claim 6, which is characterized in that the temperature of first order seed culture is 30- in step (1)
35 DEG C, incubation time 20-30h.
8. preparation method according to claim 6, which is characterized in that the temperature of step (2) second level culture is 30-35 DEG C,
Incubation time is 2-4d.
9. preparation method a method according to any one of claims 6-8, which is characterized in that incubation time is 72- in step (3)
144h, solid fermentation culture medium include: 15-25 parts of sweet sorghum stalk powder in parts by weight, and 20-30 parts of wheat bran, 5-15 parts of rice bran,
15-25 parts of bean cake powder, 10-20 parts of starch, 3-8 parts of medical stone, 0.5-2 parts of yeast powder, 2-4 parts of calcium carbonate, magnesium sulfate 0.3-0.8
Part, 0.5-2 parts of potassium dihydrogen phosphate, 0.3-0.8 parts of manganese sulfate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610887157.XA CN106434458B (en) | 2016-10-11 | 2016-10-11 | A kind of Paenibacillus polymyxa and its microbial inoculum and preparation and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610887157.XA CN106434458B (en) | 2016-10-11 | 2016-10-11 | A kind of Paenibacillus polymyxa and its microbial inoculum and preparation and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106434458A CN106434458A (en) | 2017-02-22 |
| CN106434458B true CN106434458B (en) | 2019-11-26 |
Family
ID=58174943
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610887157.XA Active CN106434458B (en) | 2016-10-11 | 2016-10-11 | A kind of Paenibacillus polymyxa and its microbial inoculum and preparation and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106434458B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107937326B (en) * | 2018-01-17 | 2020-11-24 | 鲁东大学 | Paenibacillus polymyxa for preventing and treating root rot of American ginseng and application thereof |
| CN108294049B (en) * | 2018-01-19 | 2019-07-19 | 江西省科学院微生物研究所 | A kind of Paenibacillus polymyxa culture solution combination drug and preparation method thereof for treating Citrus Huanglongbing pathogen |
| CN110241051B (en) * | 2019-07-04 | 2020-11-24 | 临沂大学 | Mixed microbial community for promoting growth of plant stems |
| CN111713206A (en) * | 2020-06-19 | 2020-09-29 | 四川省农业科学院植物保护研究所 | Method for increasing yield of bitter gourds by using biocontrol strain bacillus amyloliquefaciens Bam22 |
| CN112501056A (en) * | 2020-11-10 | 2021-03-16 | 北京航天恒丰科技股份有限公司 | Solid fermentation medium and method for producing bacillus pumilus microbial inoculum by using same |
| CN113930371B (en) * | 2021-11-23 | 2023-05-30 | 广西壮族自治区农业科学院 | Method for promoting vegetable growth and disease resistance by using paenibacillus polymyxa AF01 |
| CN115777727B (en) * | 2022-11-21 | 2023-12-12 | 浙江大学山东(临沂)现代农业研究院 | Microbial agent for resisting root rot and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102086444A (en) * | 2010-12-09 | 2011-06-08 | 南京师范大学 | Paenibacillus elgii strain and application thereof |
| CN104694446A (en) * | 2015-03-31 | 2015-06-10 | 江苏丘陵地区镇江农业科学研究所 | Paenibacillus polymyxa JX-13 and application thereof |
| CN105734000A (en) * | 2016-05-06 | 2016-07-06 | 南京农业大学 | Paenibacillus polymyxa NSY50 with capabilities of promoting growth and preventing diseases |
-
2016
- 2016-10-11 CN CN201610887157.XA patent/CN106434458B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102086444A (en) * | 2010-12-09 | 2011-06-08 | 南京师范大学 | Paenibacillus elgii strain and application thereof |
| CN104694446A (en) * | 2015-03-31 | 2015-06-10 | 江苏丘陵地区镇江农业科学研究所 | Paenibacillus polymyxa JX-13 and application thereof |
| CN105734000A (en) * | 2016-05-06 | 2016-07-06 | 南京农业大学 | Paenibacillus polymyxa NSY50 with capabilities of promoting growth and preventing diseases |
Non-Patent Citations (1)
| Title |
|---|
| Isolation and characterisation of fusaricidin-type compound-producing strain of Paenibacillus polymyxa SQR-21 active against Fusarium oxysporum f.sp.nevium;Waseem Raza et al;《Eur J Plant Pathol》;20090623;471-483 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106434458A (en) | 2017-02-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106434458B (en) | A kind of Paenibacillus polymyxa and its microbial inoculum and preparation and application | |
| CN106591193B (en) | One plant of bacillus amyloliquefaciens with the degeneration-resistant effect of wide spectrum growth-promoting | |
| CN102816725B (en) | Bacillus subtilis and application thereof | |
| CN102296036B (en) | Bacillus pumilus and application in disease prevention and growth promotion thereof | |
| CN104531533A (en) | Composite microbial inoculant for controlling plant continuous-cropping diseases and preparation method and application thereof | |
| CN103265958A (en) | Method for preparing commercialized organic material decomposition agent | |
| CN108277177B (en) | Streptomyces microflavus solid fermentation medium, preparation method and fermentation method thereof, fermentation product, biocontrol product and application | |
| CN103184184A (en) | Streptomyces albidoflavus and applications thereof | |
| CN101948780B (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
| CN108148778A (en) | The preparation of bacillus amyloliquefaciens GY30 and its bacterium powder and purposes | |
| CN105087399B (en) | A kind of straw-returning fiber hydrolization biocontrol fungi and microbial inoculum and application | |
| CN112746024A (en) | Trichoderma composite microbial agent and bio-organic fertilizer prepared from same | |
| CN107418906B (en) | A kind of composite bacteria agent and its application improving color green pepper yield and anti-root rot | |
| CN109315265B (en) | Preparation method and application of chaetomium globosum biological seedling culture medium | |
| CN109355197B (en) | Growth-promoting bacterium for promoting growth of saline-alkali soil alfalfa and microbial organic fertilizer thereof | |
| CN110122485A (en) | A kind of trichoderma, Trichoderma and preparation method thereof | |
| CN103382447A (en) | Streptomyces viridocyaneus Sv-29 and application thereof | |
| CN109182219A (en) | One plant of Mo Haiwei bacillus for promoting the growth of Henry David Thoreau grass and its application | |
| CN108676760A (en) | A kind of organic fertilizer decomposing agent and preparation method thereof | |
| CN105439657B (en) | A kind of preparation method of the special biologic organic fertilizer of resisting repeated stubbles of strawberry | |
| CN104403976A (en) | Antagonistic actinomycete for preventing and controlling continuously cropped strawberry root rot and preparation method of biocontrol agent of antagonistic actinomycete | |
| CN111172064B (en) | Bacillus laterosporus and application thereof | |
| CN108531404B (en) | Culture method of Fusarium proliferatum and application thereof | |
| KR20090093545A (en) | Bacillus pumilus kme9002 having anti-activity of penicillium, powder comprising the same strain and process for producing fermenting medium of waste cotton using the same | |
| CN113213986A (en) | Biological organic fertilizer containing bacillus amyloliquefaciens and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |