CN106434435A - Acetobacter indonesiensis and application in accelerating separation and settlement of mung bean starch - Google Patents
Acetobacter indonesiensis and application in accelerating separation and settlement of mung bean starch Download PDFInfo
- Publication number
- CN106434435A CN106434435A CN201610814812.9A CN201610814812A CN106434435A CN 106434435 A CN106434435 A CN 106434435A CN 201610814812 A CN201610814812 A CN 201610814812A CN 106434435 A CN106434435 A CN 106434435A
- Authority
- CN
- China
- Prior art keywords
- acetobacter
- starch
- lly11
- green
- bean juice
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/02—Acetobacter
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
- C08B30/04—Extraction or purification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Materials Engineering (AREA)
- Polymers & Plastics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses acetobacter indonesiensis LLY11, which is preserved in China General Microbiological Culture Collection Center on July 15, 2016, with preservation number of No. 12794. Pure fermented acid pulp of the LLY11, when applied to the production of the mung bean starch, has advantages of accelerating a starch settlement speed, shortening a starch settling time and improving a starch yield; and the acetobacter indonesiensis LLY11 fermented acid pulp is subjected to pure fermentation, which is free from pollution caused by pathogenic bacteria such as infectious microbes and the like, so that the eating safety of starch products is enhanced.
Description
Technical field
The present invention relates to one plant of acetobacter and the application in accelerating green starch separation to settle.
Technical background
Acetic acid bacteria can improve the local flavor of food, quality and nutrition, extend the shelf life, and have regulation human body intestinal canal flora and put down
The important Functions of Physiological Health Cares such as weighing apparatus, the growth of suppression pathogen, reduction cholesterol, enhance immunity, anti-aging, extensive
It is applied to food service industry, acetic acid bacteria fermented food is also considered as functional food.At present, the production of green starch adopts nature
Settled process or acid slurry method, settled process is not only time-consuming but also starch removal is not thorough；Acid slurry method starch separation effect is good compared with settled process,
But because wintercherry is the product of spontaneous fermentation, affected by the environmental factors such as raw material, climate change and method of operating very big, produced
Quality is very unstable, sometimes due to temperature or other factors cause yeast-bitten, wintercherry quality deteriorates；Sometimes wintercherry vigor is not
Foot is so that starch can not timely and effective with protein and other impurities separate.Cause these problems be primarily due to right
In wintercherry, the Main Function bacterium understanding of precipitation and separation starch is not also fully aware of, therefore also never artificial preparation, can
The leavening exploitation producing for wintercherry, can't carry out manual fermentation.
Content of the invention：
The purpose of the present invention is aiming at green starch precipitation and separation time length, starch separation precipitates the thorough, quality of product
Unstable the problems such as, one plant is provided to promote the strong acetobacter of starch separation sedimentation capacity, this acetobacter can be green in acceleration
Play a key effect during beans starch sedimentation separation.
Another object of the present invention is to, application in accelerating starch separation sedimentation for this acetobacter, improves starch
Yield, makes the stability of product improve.Meanwhile, the wintercherry in the present invention is sterile fermentation, the no pathogenic bacteria such as miscellaneous bacteria pollution, improves
The edible safety of starch product.
The present invention is achieved by the following technical solutions：
The present invention provides one plant of acetobacter promoting starch separation sedimentation, and this acetobacter is acetobacterAcetobacter indonesiensisLLY11, is preserved in China Committee for Culture Collection of Microorganisms on July 15th, 2016 common
Microorganism center, deposit number is CGMCC No. 12794.
The individual morphology feature of described acetobacter bacterial strain：Size is（0.8 m～1.2 m）×（1.5 m～2.5
m）, cell is oval or quarter butt, Gram-negative, no gemma.Colony morphology characteristic：Bacterium colony size in 2 mm～3mm, milk yellow,
Opaque, colony edge is neat, smooth surface, moistening glossy, and protuberance is in hemispherical.Physiological and biochemical property is：Oxytolerant, peroxide
Change hydrogen enzyme positive it is impossible to motion, hydrogen sulfide, nitrate reduction, gelatin experiment feminine gender, metabolizable glucose produces acid not aerogenesis, can generation
Thank fructose, sucrose, lactose, galactolipin, maltose, mannose, sorbierite, rhamnose, melezitose, melibiose, cellobiose product
Acid is it is impossible to utilize raffinose, arabinose.
16 S rDNA sequence totally 1411 bp of this bacterial strain, through Genbank compare withAcetobacter indonesiensisSimilitude reaches 99%；In conjunction with its individual, colony morphology feature and bio-chemical characteristics result, identify this bacterium
ForAcetobacter indonesiensis, and be named asAcetobacter indonesiensisLLY11.
AcetobacterAcetobacter indonesiensisThe preparation of LLY11 fermentation wintercherry：With oese picking three
The acetobacter of ring slant preservationAcetobacter indonesiensisLLY11 in 5 mL green bean juice culture mediums, 30
After DEG C bottom fermentation 12 h, by this 5mL zymotic fluid Amplification Culture, continue to access 30 DEG C of bottom fermentations 12 in 100 mL green bean juice culture mediums
H, obtainsAcetobacter indonesiensisThe seed culture fluid of LLY11.By 5%-8% inoculum concentration by seed culture fluid
It is linked in green bean juice culture medium, 30 DEG C of bottom fermentation 12 h, you can obtain the wintercherry for settling green starch breast.
AcetobacterAcetobacter indonesiensisLLY11 fermentation application in sedimentation starch for the wintercherry：Will
Above-mentionedAcetobacter indonesiensisLLY11 sterile fermentation obtain wintercherry by volume 10% amount be added to green
In beans starch milk, stir 1-2min rapidly, then stand.This bacterial strain can be specifically bound to starch particle surface, will be numerous
Starch granules is coupled to big flocculation group, and the starch granules gravity due to being bound up increases, and accelerates starch and protein
Deng the separation sinking speed of impurity, can be by sinking speed by 20min（Needed for precipitation 10mL starch in 100mL green starch breast
Time）Foreshorten to 2min.
Acetobacter indonesiensisLLY11 pure-blood ferment wintercherry is formed sediment in mung bean with spontaneous fermentation mung bean wintercherry
Powder is applied to advantages below in producing：
(1) improve starch sinking speed, shorten the starch sedimentation time, improve starch yield；
⑵Acetobacter indonesiensisLLY11 fermentation wintercherry is sterile fermentation, the no pathogenic bacteria such as miscellaneous bacteria pollution,
Improve the edible safety of starch product.
Specific embodiment
Embodiment 1
Starch is accelerated to settle the separation of microorganism, screening and identification
1 culture medium
(1) green bean juice culture medium prescription：Yeast extract 5 g, K2HPO42g, glucose 20 g, lactose 2 g, sodium acetate 5 g, mung bean
Juice 1000 mL.
(2) the preparation method of green bean juice：100 g mung beans are taken to add distilled water to 1000 mL, heating is boiled 20min, used 120
Purpose screen filtration, obtains final product green bean juice.
(3) isolation medium：Using soluble starch substitute green bean juice culture medium in glucose as carbon source, concrete composition and
Its outfit is as follows：Yeast extract 5 g, K2HPO42g, soluble starch 20 g, lactose 2 g, sodium acetate 5 g, green bean juice 1000 mL.
Adjust pH to 6.8,115 DEG C of sterilizing 30 min.
2 detection methods
The detection method of 2.1 green starch sinking speed：
Bubble is taken to send out the mung bean of 8-12h, by 1:15 ratio adds water making beating, takes the green starch breast several pieces that 100mL accomplishes fluently, respectively
Add spontaneous fermentation wintercherry, sterile fermentation liquid and blank green bean juice culture medium, measure respectively required for sedimentation 10mL starch when
Between.Every group is done 3 repetitions.
2.2 the detection method of coliform：
With reference to GB 47893-2010《National food safety standard food microbiological examination enumeration of coliforms》.
3 accelerate starch sedimentation microorganism separation method
The dilution of 3.1 samples
Draw mung bean wintercherry 5mL of spontaneous fermentation with aseptic straw, move into and fill 45mL SPSS with the three of bead
In the bottle of angle, shake well is uniform, and as 10-1Sample diluting liquid.With SPSS to 10-1Sample diluting liquid carry out
Gradient dilution is to 10-7.
3.2 plate isolation
Take above-mentioned 10-4、10-5、10-6、10-7The each 1mL of dilution dilution is injected separately in sterile petri dish, each dilution factor
Do 3 repetitions.Injection isolation medium, after culture medium solidifying, is inverted in culture 24h～48h in 30 DEG C of incubators.
3.3 plating cultures
With the single petite of oese picking, streak inoculation in isolation medium flat board, 30 DEG C of culture 24h, repeatedly purify 3
In generation, obtain single bacterium colony and be inoculated on green bean juice slant medium, 30 DEG C of culture 24h.
3.4 bacterial strain screening
Access after pure for the inclined-plane being separated to bacterial strain is activated and substitute in the green bean juice nutrient solution of glucose with soluble starch, 30 DEG C
Culture 24h, with the acceleration starch separation sinking speed of zymotic fluid as index, filters out one plant of rush starch separation sinking speed high
Bacterial strain LLY11.
3.5 strain idenfication
3.5.1 the morphological feature of bacterial strain LLY11 is observed
By bacterial strain LLY11 30 DEG C in green bean juice agar medium at cultivate 24h, then carry out Gram's staining, its bodily form
State feature is shown in Table 1.3.5 strain idenfication
3.5.1 the morphological feature of bacterial strain LLY11 is observed
By bacterial strain LLY11 30 DEG C in green bean juice agar medium at cultivate 24h, then carry out Gram's staining, its bodily form
State feature is shown in Table 1.
The individual morphology feature of table 1 LLY11 bacterial strain
| Bacterial strain | Thalline size | Cellular morphology | Arrangement mode after cell division | Gram's staining | Gemma | Motility |
| LLY11 | 0.8～1.2 m）×（1.5 m～2.5 m） | Cell ellipse or quarter butt | Chain | Gram-negative | No | Do not move |
Bacterial strain LLY11, in green bean juice agar medium, cultivates 48h at 30 DEG C, observes bacterium colony size, form and color, and result is shown in
Table 2.
Table 2 colony morphology characteristic
3.5.2 the physiological and biochemical property of LLY11
According to customary physiological biochemical identification, the results are shown in Table 3, reference《The outstanding Bacteria Identification handbook of uncle》, tentatively judge that bacterial strain LLY11 is
Acetobacter.
Table 3 bacterial strain physio-biochemical characteristics
| Test event | Result | Test event | Result |
| Catalase | + | Lactose | + |
| Mobility test | - | Galactolipin | + |
| H2S tests | - | Maltose | + |
| Nitrate reduction | - | Mannitol | + |
| Gelatin liquefaction | - | Sorbierite | + |
| 15 DEG C of growths | + | Gossypose | - |
| 45 DEG C of growths | - | Mannose | + |
| Gluconate | + | Rhamnose | + |
| Glucose aerogenesis | - | Melezitose | + |
| Glucose produces acid | + | Melibiose | + |
| Wood sugar | - | Seven leaf sugar | + |
| Fructose | + | Arabinose | - |
| Sucrose | + | Cellobiose | + |
3.5.3 the 16S rDNA the sequencing results of acetobacter LLY11
The common 1411bp of 16 S rDNA sequence of this bacterial strain, through Genbank compare withAcetobacter indonesiensisSimilitude reaches 99%, and its colony morphology comprehensive is observed, individual morphology is observed, physiological and biochemical test result,
Determine that this bacterial strain isAcetobacter indonesiensisAnd be named asAcetobacter indonesiensis
LLY11, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 15th, 2016（Referred to as
CGMCC, address is：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, institute of microbiology of the Chinese Academy of Sciences, postcode 100101）, preservation volume
Number be CGMCC No. 12794.
Embodiment 2
Acetobacter indonesiensisApplication in starch processing for the LLY11 fermentation wintercherry
1 experiment material
The bacterial strain of test is that the present invention is detachedAcetobacter indonesiensisLLY11, Starch Production raw material selects
Select mung bean.
2Acetobacter indonesiensisThe preparation of LLY11 fermentation wintercherry
(1) green bean juice culture medium prescription：Yeast extract 5g, K2HPO42g, glucose 20g, lactose 2g, sodium acetate 5g, green bean juice
1000mL.
(2) the preparation method of green bean juice：100g mung bean is taken to add distilled water to 1000mL, heating is boiled 20min, used 120 mesh
Screen filtration, obtain final product green bean juice.
(3) acetobacter LLY11 seed culture liquid and preparation method thereof：Acetobacter with oese picking three ring slant preservation
After LLY11 30 DEG C of bottom fermentation 12h in the 5mL green bean juice culture medium, by this 5mL zymotic fluid Amplification Culture, continue to access 100mL green
30 DEG C of bottom fermentation 12h in fermented bean drink culture medium, obtain the seed culture fluid of acetobacter LLY11.
(4) acetobacter LLY11 seed culture fluid is linked in green bean juice culture medium by the inoculum concentration of 5%-8%, 30 DEG C
Bottom fermentation 12h, you can obtain the fermentation wintercherry for settling green starch breast.
3 are applied to accelerate starch to separate sedimentation with impurity such as protein
WillAcetobacter indonesiensisLLY11 fermentation obtain wintercherry by volume 10% amount be added to mung bean
In starch milk, stir 1-2min rapidly, then stand.
Take addition respectivelyAcetobacter indonesiensisGreen starch breast before and after LLY11 zymotic fluid, aobvious
The distribution of micro- Microscopic observation starch granules, is shown in Fig. 1, Fig. 2, as seen from the figure,Acetobacter indonesiensis
LLY11 can be specifically bound to starch particle surface, and numerous starch granules are coupled to big flocculation group.Due to being bound up on one
Rise starch granules gravity increase, accelerate starch and separate sinking speed with impurity such as protein, can by sinking speed by
20min（10mL starch required time is precipitated in 100mL green starch breast）Foreshorten to 2min.
Brief description
Fig. 1 is to observe the green starch distribution of particles figure adding before zymotic fluid under 100 power microscopes；
Fig. 2 is addition 10%Acetobacter indonesiensisAfter LLY11 fermentation wintercherry, green starch particle is coupled to
Big flocculation group.
Effect of settling is relatively shown in Table 4；
The amount detection of 4 coliforms:
Right respectivelyAcetobacter indonesiensisColiform in LLY11 fermentation wintercherry and spontaneous fermentation mung bean wintercherry
Quantity detected, result such as table 5 below:
The present invention filters out one plant from spontaneous fermentation mung bean wintercherry and can accelerate starch sedimentationAcetobacter indonesiensisLLY11, and be applied in mung bean fermented wintercherry, it is heavy that its zymotic fluid can accelerate green starch to separate
Fall.This invention not only shortens the time of starch separation sedimentation, improves starch yield, so that the stability of product is improved；And
Wintercherry in invention is sterile fermentation, the no pathogenic bacteria such as miscellaneous bacteria pollution, improves the edible safety of starch product.
SEQUENCE LISTING
<110>Jinzhou medical university
<120>One plant of acetic acid bacteria promoting starch separation sedimentation
<130>Separate the application in settling accelerating green starch
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1411
<212> DNA
<213> Acetobacter indonesiensis
<400> 1
gtggtcggct gcgccccttg cgggttcgct caccggctta aggtcaaacc aactcccatg 60
gtgtgacggg cggtgtgtac aaggcccggg aacgtattca ccgcggcatg ctgatccgcg 120
attactagcg attccacctt catgcactcg agttgcagag tgcaatccga actgagacgg 180
cttttagaga tcagcacgat gtcgccatct agcttcccac tgtcaccgcc attgtagcac 240
gtgtgtagcc caggacataa gggccatgag gacttgacgt catccccacc ttcctccggc 300
ttgtcaccgg cagtctctct agagtgccca cccaaacatg ctggcaacta aagatagggg 360
ttgcgctcgt tgcgggactt aacccaacat ctcacgacac gagctgacga cagccatgca 420
gcacctgtgc ggtaggtccc ttgcgggaaa tatccatctc tgaatacagc ctacccatac 480
aagccctggt aaggttctgc gcgttgcttc gaattaaacc acatgctcca ccgcttgtgc 540
gggcccccgt caattccttt gagtttcaac cttgcggccg tactccccag gcggtgtgct 600
tatcgcgtta gctacgacac tgaataacta agttacccaa catccagcac acatcgttta 660
cagcgtggac taccagggta tctaatcctg tttgctcccc acgctttcgc gcctcagcgt 720
cagtaatgag ccaggttgcc gccttcgcca ccggtgttct tcccaatatc tacgaatttc 780
acctctacac tgggaattcc acaaccctct ctcacactct agtctgcacg tctcaaatgc 840
agctcccagg ttaagcccgg ggatttcaca tctgactgta caaaccgcct acacgccctt 900
tacgcccagt cattccgagc aacgctagcc cccttcgtat taccgcggct gctggcacga 960
agttagccgg ggcttcttct gcgggtaccg tcatcatcgt ccccgccgaa agtgctttac 1020
aatccgaaaa ccttcttcac acacgcggca ttgctggatc agggttgccc ccattgtcca 1080
atattcccca ctgctgcctc ccgtaggagt ctgggccgtg tctcagtccc agtgtggctg 1140
atcatcctct cagaccagct attgatcatc gccttggtag gccattaccc caccaacaag 1200
ctaatcaaac gcaggctcct ccgcaggcga cttgcgcctt tgaccctcag gtgtcatgcg 1260
gtattagctc cagtttccca gagttatccc ccacccacgg atagattcct acgcgttact 1320
cacccgtccg ccactaaggc cgaaaccttc gtgcgacttg catgtgttaa gcatgccgcc 1380
agcgttcgct ctgagccagg attcaaacac t 1411
Claims (3)
1. one plant accelerates the acetic acid bacteria of starch separation sedimentation it is characterised in that this bacterium is acetobacterAcetobacter indonesiensisLLY11, is preserved in China Committee for Culture Collection of Microorganisms on July 15th, 2016 commonly micro-
Bio-Centers, deposit number is CGMCC No. 12794.
2. one plant according to claim 1 accelerates the acetic acid bacteria of starch separation sedimentation it is characterised in that acetobacterAcetobacter indonesiensisThe fermentation wintercherry of LLY11 is applied in green starch separates.
3. the one plant according to claim 2 acetic acid bacteria accelerating starch separation sedimentation, is characterized in that, separates in green starch
In, acetobacter usedAcetobacter indonesiensisLLY11 fermentation wintercherry adopts following method preparation：
(1) green bean juice culture medium prescription：Yeast extract 5g, K2HPO42g, glucose 20g, lactose 2g, sodium acetate 5g, green bean juice
1000 mL；
(2) the preparation method of green bean juice：100g mung bean is taken to add distilled water to 1000 mL, heating is boiled 20 min, used 120 purposes
Screen filtration, obtains final product green bean juice；
(3) acetobacter LLY11 seed culture liquid and preparation method thereof：Acetobacter with oese picking three ring slant preservation
After LLY11 30 DEG C of bottom fermentation 12 h in the 5 mL green bean juice culture mediums, by this 5mL zymotic fluid Amplification Culture, continue access 100
30 DEG C of bottom fermentation 12 h in mL green bean juice culture medium, obtain the seed culture fluid of acetobacter LLY11；
(4) acetobacter LLY11 seed culture fluid is linked in green bean juice culture medium by the inoculum concentration of 5%-8%, 30 DEG C issue
Ferment 12 h, you can obtain the wintercherry for settling green starch breast.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610814812.9A CN106434435B (en) | 2016-09-09 | 2016-09-09 | One plant of acetobacter and the application in acceleration green starch separation sedimentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610814812.9A CN106434435B (en) | 2016-09-09 | 2016-09-09 | One plant of acetobacter and the application in acceleration green starch separation sedimentation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106434435A true CN106434435A (en) | 2017-02-22 |
| CN106434435B CN106434435B (en) | 2019-04-26 |
Family
ID=58167629
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610814812.9A Active CN106434435B (en) | 2016-09-09 | 2016-09-09 | One plant of acetobacter and the application in acceleration green starch separation sedimentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106434435B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111304128A (en) * | 2020-03-09 | 2020-06-19 | 西南交通大学 | Compound microbial agent for treating kitchen grease wastewater and preparation method and application thereof |
| CN112852681A (en) * | 2021-03-17 | 2021-05-28 | 锦州医科大学 | Lactobacillus fermentum, application of lactobacillus fermentum, fermented sour pulp, preparation method of fermented sour pulp and starch precipitator |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103194409B (en) * | 2013-04-03 | 2014-09-17 | 天地壹号饮料股份有限公司 | Bacillus aceticus and application thereof in preparing apple vinegar |
| CN103876005B (en) * | 2014-03-19 | 2015-12-02 | 湖北工业大学 | A kind of composite bacteria agent capable promoting the sedimentation of starch from sweet potato slurries |
| CN104041726B (en) * | 2014-05-21 | 2015-11-18 | 湖北工业大学 | A kind of composite bacteria agent capable promoting starch sedimentation in Lotus root congee slurries |
-
2016
- 2016-09-09 CN CN201610814812.9A patent/CN106434435B/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111304128A (en) * | 2020-03-09 | 2020-06-19 | 西南交通大学 | Compound microbial agent for treating kitchen grease wastewater and preparation method and application thereof |
| CN112852681A (en) * | 2021-03-17 | 2021-05-28 | 锦州医科大学 | Lactobacillus fermentum, application of lactobacillus fermentum, fermented sour pulp, preparation method of fermented sour pulp and starch precipitator |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106434435B (en) | 2019-04-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102533588B (en) | Lactobacillus brevis for producing extracellular exopolysaccharide and application thereof | |
| CN102453689A (en) | Lactobacillus plantarum strain producing extracellular polysaccharide, and application thereof | |
| CN111676156B (en) | Bacillus belgii MRS for improving reduction activity and fermentation product and application thereof | |
| CN110317757A (en) | Lactobacillus plantarum HJ-S2 and its application of one plant of tool norcholesterol and selenium enriching functions | |
| CN110093285B (en) | Acid-resistant lactobacillus fermentum and application thereof | |
| CN103060243A (en) | Sub-lactobacillus casei and sub-cheese subspecies strain | |
| CN112574923A (en) | Streptococcus thermophilus capable of producing exopolysaccharides in high yield and application thereof | |
| CN106754507B (en) | Compound flavor microbial inoculum, preparation method thereof and direct-throwing application thereof in soy sauce flavoring | |
| CN108018247B (en) | High-yield straight-chain glucan strain and glucan fermentation production method thereof | |
| CN106119166B (en) | One plant of Switzerland lactic acid bacteria and its application | |
| CN109645490A (en) | Application of the lactobacillus plantarum CQPC02 in the food or drug of preparation prevention diabetes | |
| CN106434435A (en) | Acetobacter indonesiensis and application in accelerating separation and settlement of mung bean starch | |
| CN113337446A (en) | Preparation method and application of composite leavening agent | |
| CN115895973B (en) | Lactobacillus paracasei and application thereof in fermentation preparation of white sour soup | |
| CN114350553B (en) | Bacillus amyloliquefaciens capable of producing protease at high yield and application thereof | |
| CN112852681B (en) | Lactobacillus fermentum, application of lactobacillus fermentum, fermented sour pulp, preparation method of fermented sour pulp and starch precipitator | |
| CN115418337B (en) | Lignin degrading bacterium and application thereof in rice straw micro-storage | |
| CN113308419B (en) | Lactobacillus chaff for fermentation and application thereof | |
| CN113308418B (en) | Lactobacillus chaff for fermentation and fermentation preparation process thereof | |
| CN108239616A (en) | One Enterococcus faecalis bacterial strain and its application in wintercherry Tofu processing | |
| CN113801800A (en) | Saccharomyces cerevisiae and application thereof | |
| CN110055189B (en) | Lactobacillus plantarum YL15 and application thereof in red wine flavor yoghourt | |
| CN111154667B (en) | High-density culture method of lactic acid bacteria with starch coagulation effect | |
| CN113699069A (en) | Strain HSCY2073, separation and screening thereof and application of strain to improvement of flavor quality of vinegar | |
| CN108330082B (en) | Lactobacillus paracasei and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Xu Yunhe Inventor after: Zhang Lili Inventor after: Liu Liying Inventor after: Yu Yang Inventor after: Li Jingshuang Inventor before: Zhang Lili Inventor before: Xu Yunhe Inventor before: Liu Liying Inventor before: Yu Yang Inventor before: Li Jingshuang |
|
| CB03 | Change of inventor or designer information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |