CN106434337B - A kind of ultrasonic cell-break device based on spherical wave resonance principle - Google Patents
A kind of ultrasonic cell-break device based on spherical wave resonance principle Download PDFInfo
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
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Abstract
The invention discloses a kind of ultrasonic cell-break devices based on spherical wave resonance principle, including the supersonic generator, energy converter and amplitude transformer connected by transmission line, the side of supersonic generator is equipped with media Containers, energy converter is arranged between supersonic generator and media Containers, amplitude transformer connect with energy converter and is arranged in media Containers, sample container is equipped in media Containers, sample container is fixed in media Containers by the fixed device of sample container.The advantage of the invention is that, under the premise of not improving supersonic generator energy, the characteristics of spherical wave is formed in sample container using ultrasonic wave and is enriched with, realize the reinforcing to ultrasonic wave tissue crushing effect, the broken effect of material is effectively raised, will not influence normally broken work, sample container can take out out of media Containers, the cleaning for facilitating media Containers avoids the phenomenon that media fluid remains in media Containers.
Description
Technical field
The invention belongs to ultrasonic disruption equipment technical fields, and in particular to a kind of ultrasound based on spherical wave resonance principle
Wave cell breaking plant.
Background technique
Ultrasonic disruption device is so that liquid is generated numerous minute bubbles using the cavitation effect of ultrasonic wave in a liquid,
By the explosion of minute bubbles, so that solid particle or cell tissue in liquid be made to be crushed, it can be used for a variety of animals and plants, disease
Poison, cell, bacterium and tissue it is broken, while can also be used to emulsification, it is discrete, homogenize, extract, defoaming, clearly, nano material
Preparation, dispersion and acceleration chemical reaction etc., ultrasonic disruption device is widely used in biology, microbiology, physics, animal
The fields such as, agronomy, pharmacy, chemical industry, sewage treatment, nano material.
Traditional ultrasonic disruption device is made of three supersonic generator, energy converter and sound proof box parts, energy converter
At work because vibration can generate biggish noise, in actual use, generally all energy converter is put into sound proof box, it can
It is effectively isolated noise, has a dedicated broken bottle in sound proof box, the amplitude transformer on energy converter is protruded into the liquid in broken bottle
In, to carry out fragmentation procedure to cell or tissue, the structure setting of traditional ultrasonic disruption device is unreasonable, is unfavorable for locating
The heat dissipation of liquid is managed, treatment fluid can remain at the dead angle of broken bottle, be not easy to the cleaning of broken bottle.
Ultrasonic cell-break device has been widely used in scientific experiment and actual production, by ultrasonic wave in liquid
Cavitation effect in body causes the covibration in material structure, and the structure of material is destroyed using resonance, currently used super
Sonication device is mainly based on scattered wave and T-type wave, and scattered wave range is wide, and intensity is relatively weak, although T-type wave is strong
Degree is higher, but spreads around rapidly after its generation, not will form the accumulation of Wave energy, crushing effect depends primarily on wave
First set reaction after dynamic impact causes material crushing effect bad since Wave energy cannot be assembled, and influences the experiment in later period
And production and processing, working efficiency are low.
Summary of the invention
The object of the present invention is to provide a kind of ultrasonic cell-break devices based on spherical wave resonance principle, existing to solve
There is Wave energy in technology that cannot assemble, material crushing effect is bad, influences the experiment and production and processing in later period, working efficiency
The problem of low, media fluid heat dissipation is bad, and media fluid residual is not easy to cleaning.
In order to achieve the above objectives, the technical scheme is that a kind of supersonic cell based on spherical wave resonance principle
Crushing device, supersonic generator 2, energy converter 3 and the amplitude transformer 4 connected including passing through transmission line 1, supersonic generator 2
Side is equipped with media Containers 5, and energy converter 3 is arranged between supersonic generator 2 and media Containers 5, amplitude transformer 4 and energy converter 3
It connects and is arranged in media Containers 5, sample container 6 is equipped in media Containers 5, sample container 6 is filled by the way that sample container is fixed
It sets and is fixed in media Containers 5.
Preferably, sample container 6 is spherical container, and sample container 6 is equipped with feeder pipe 7, one end of feeder pipe 7 and sample
Container 6 is fixedly connected, and the other end is extended on outside media Containers 5.
Preferably, the fixed device of sample container includes fixed frame 8, connecting rod 9 and stop collar 10, fixed frame 8 and is situated between
The inner wall of matter container 5 is adapted, and the internal diameter of stop collar 10 is adapted with the outer diameter of sample container 6, and fixed frame 8 passes through connecting rod
9 are fixedly connected with stop collar 10.
Preferably, jacking block 11 compatible with sample container 6, the side of jacking block 11 and sample container 6 are equipped in connecting rod 9
It is in contact, the other side is connected with spring 12.
Preferably, stop collar 10 and the interconnecting piece of connecting rod 9 are equipped with bottom compatible with sample container 6 gag lever post 13.
Preferably, the inner wall of media Containers 5 is equipped with limited block 14.
Preferably, it is filled with external agency 15 in media Containers 5, interior media 16 is filled in sample container 6, outside is situated between
The tall and big spherical portion in sample container 6 of the liquid of matter 15, the tall and big spherical portion in sample container 6 of the liquid of interior media 16 and is less than outer
The liquid of portion's medium 15 is high.
Preferably, the distance of sample container 6 to amplitude transformer 4 is the integral multiple of ultrasonic frequency wavelength in external agency 15,
The internal diameter of sample container 6 is the integral multiple of ultrasonic frequency wavelength in interior media 16, and the wall thickness of sample container 6 is less than internal be situated between
The 1/10 of ultrasonic frequency wavelength in 16 material therefor of matter, the density of external agency 15 are greater than the density of interior media 16.
Preferably, the top of media Containers 5 is equipped with top cover 17, and top cover 17 is connected by hinge 18 and 5 activity of media Containers
It connects.
Preferably, top cover 17 be equipped with feeder pipe 7 can by through-hole 19, be socketed with feeder pipe sealing ring on through-hole 19
20, the side plate of media Containers 5 is equipped with top seal pad 21 corresponding with top cover 17.
The present invention has the advantages that the present invention is existed under the premise of not improving supersonic generator energy using ultrasonic wave
The characteristics of forming spherical wave in sample container and being enriched with, realizes the reinforcing to ultrasonic wave tissue crushing effect, effectively
Improve the broken effect of material, ultrasonic energy will not be spread around, and sample container passes through sample container fixation device
It is firmly fixed in media Containers, avoids the phenomenon that sample container is subjected to displacement, ensure that the stabilization of material shattering process
Property, the working efficiency of crushing device is effectively raised, provides convenience, inside and outside two media for the experiment and production and processing in later period
Good heat dissipation effect, will not influence normally broken work, sample container can take out out of media Containers, facilitate media Containers
Cleaning, avoids the phenomenon that media fluid remains in media Containers.
Detailed description of the invention
Fig. 1 is schematic cross-sectional view of the invention.
Fig. 2 is the structural schematic diagram of sample of the present invention receptacle fixture.
Fig. 3 is section view schematic perspective view of the invention.
Appended drawing reference meaning is as follows: transmission line (1), supersonic generator (2), energy converter (3), amplitude transformer (4), and medium holds
Device (5), sample container (6), feeder pipe (7), fixed frame (8), connecting rod (9), stop collar (10), jacking block (11), spring
(12), bottom gag lever post (13), limited block (14), external agency (15), interior media (16), top cover (17), hinge (18) lead to
Hole (19), feeder pipe sealing ring (20), top seal pad (21).
Specific embodiment
The invention will be further described with specific embodiment for explanation with reference to the accompanying drawing.
A kind of ultrasonic cell-break device based on spherical wave resonance principle as shown in Figure 1, Figure 2 and Figure 3, including it is logical
Supersonic generator 2, energy converter 3 and the amplitude transformer 4 of the connection of transmission line 1 are crossed, the side of supersonic generator 2 is equipped with media Containers
5, energy converter 3 is arranged between supersonic generator 2 and media Containers 5, and amplitude transformer 4 connect with energy converter 3 and medium is arranged in
In container 5, sample container 6 is equipped in media Containers 5, sample container 6 is fixed on media Containers 5 by the fixed device of sample container
It is interior.
In order to make to form spherical surface resonance in sample container 6, allow ultrasonic activation in the interior media 16 of sample container 6
Interior formation enrichment improves crushing efficiency and crushing effect, and setting sample container 6 is spherical container, and sample container 6 is equipped with blanking
One end of pipe 7, feeder pipe 7 is fixedly connected with sample container 6, and the other end is extended on outside media Containers 5.
In order to guarantee stability of the sample container 6 in external agency 15, avoid 6 ontology of sample container in the work of ultrasonic wave
It is vibrated with lower, the ultrasonic wave in sample container 6 is allow to form enrichment, more firm, the sample for keeping sample container 6 fixed
Receptacle fixture includes fixed frame 8, connecting rod 9 and stop collar 10, and fixed frame 8 is adapted with the inner wall of media Containers 5,
The internal diameter of stop collar 10 is adapted with the outer diameter of sample container 6, and fixed frame 8 is fixedly connected by connecting rod 9 with stop collar 10.
In order to further ensure the stability of sample container 6, top compatible with sample container 6 is equipped in connecting rod 9
The side of block 11, jacking block 11 is in contact with sample container 6, and the other side is connected with spring 12.
In order to guarantee the stability of 6 spherical bottom of sample container, fix it more firm, in stop collar 10 and connection
The interconnecting piece of bar 9 is equipped with bottom compatible with sample container 6 gag lever post 13.
In order to guarantee the stability of the fixed device of sample container, it is facilitated to be fixed on the inner wall of media Containers 5, in medium
The inner wall of container 5 is equipped with limited block 14.
In order to further make the ultrasonic waveform in sample container 6 at enrichment, crushing effect is improved, is filled out in media Containers 5
Filled with external agency 15, interior media 16, the tall and big ball in sample container 6 of the liquid of external agency 15 are filled in sample container 6
Shape portion, the tall and big spherical portion in sample container 6 of the liquid of interior media 16 and the liquid height less than external agency 15.
In order to further increase broken efficiency and effect, so that ultrasonic wave is formed enrichment in sample container 6, improve ultrasound
The transmission speed of wave, the distance of setting sample container 6 to amplitude transformer 4 are the integer of ultrasonic frequency wavelength in external agency 15
Times, the internal diameter of sample container 6 is the integral multiple of ultrasonic frequency wavelength in interior media 16, and the wall thickness of sample container 6 is less than interior
The 1/10 of ultrasonic frequency wavelength in 16 material therefor of portion's medium, the density of external agency 15 are greater than the density of interior media 16.
Sample container 6 is taken out after making in order to facilitate breaker, is equipped with top cover 17, top cover at the top of media Containers 5
17 are flexibly connected with media Containers 5 by hinge 18.
It in order to guarantee the airtightness of media Containers, prevents external agency 15 from overflowing, makes fixed more firm of feeder pipe 7,
Top cover 17 be equipped with feeder pipe 7 can by through-hole 18, be socketed with feeder pipe sealing ring 19 on through-hole 18, media Containers 5
Side plate is equipped with top seal pad 20 corresponding with top cover 17.
Embodiment one
The power of supersonic generator 2 is 100W, and the frequency of ultrasonic wave is 20kHZ, and external agency 15 is in media Containers 5
The conduction velocity of water, the ultrasonic wave in water is 1473m/s, and wavelength 7.365cm, the distance of sample container 6 to amplitude transformer 4 is outer
The integral multiple of ultrasonic frequency wavelength in portion's medium 15, so that the distance that sample container 6 is arranged to amplitude transformer 4 is 73.65cm；It is interior
Portion's medium 16 is ethyl alcohol, and the conduction velocity of the sound wave in ethyl alcohol is 1168m/s, and the internal diameter of wavelength 5.84cm, sample container 6 are
The integral multiple of ultrasonic frequency wavelength in interior media 16, so that the internal diameter that sample container 6 is arranged is 58.4cm；Sample container 6
Material be glass, the conduction velocity of the ultrasonic wave in glass is 5640m/s, and the wall thickness of wavelength 28.2cm, sample container 6 are small
The 1/10 of ultrasonic frequency wavelength in 16 material therefor of interior media, so that the wall thickness that sample container 6 is arranged is less than 2.82cm.
By taking DPPH is in the dissolution in ethyl alcohol as an example, DPPH is the important examination for analyzing antioxidant free radical scavenging ability
Agent, it is not soluble in water, it is dissolvable in water ethyl alcohol, however its rate of dissolution is extremely slow, 0.1gDPPH is dissolved completely in 250ml ethyl alcohol about
It is 6 hours time-consuming；T-type ultrasonic unit Same Efficieney, frequency output condition under (100W, 20kHZ), need 30 minutes can be complete
Dissolve DPPH；Using the ultrasonication condition in the embodiment of the present invention one, it can effectively accelerate dissolution of the DPPH in ethyl alcohol
Rate can be completely dissolved for 5 minutes, the Particle Breakage of DPPH and dissolved efficiency effectively can be improved 6 times.
Embodiment two
The power of supersonic generator 2 is 200W, ultrasonic frequency 25kHZ, and the external agency 15 in media Containers 5 is
20% calcium chloride solution, the conduction velocity of the ultrasonic wave in calcium chloride solution are 1531m/s, wavelength 6.124cm, sample container 6
Distance to amplitude transformer 4 is the integral multiple of ultrasonic frequency wavelength in external agency 15, so that sample container 6 be arranged to amplitude transformer
4 distance is 61.24cm；Interior media 16 is water, and the conduction velocity of the ultrasonic wave in water is 1473m/s, and wavelength is
5.892cm, the internal diameter of sample container 6 is the integral multiple of ultrasonic frequency wavelength in interior media 16, so that sample container 6 be arranged
Internal diameter be 58.92cm；The material of sample container 6 is organic glass, and the conduction velocity of the ultrasonic wave in organic glass is
2692m/s, wavelength 10.77cm, the wall thickness of sample container 6 are less than ultrasonic frequency wavelength in 16 material therefor of interior media
1/10, so that the wall thickness that sample container 6 is arranged is less than 1.07cm.
For through the heparin sodium in ultrasound-assisted extraction ox lung, T-type ultrasonic unit is in Same Efficieney, frequency
Under output condition (200W, 25kHZ), heparin sodium recovery rate can be improved 16%；At the ultrasonic wave in the embodiment of the present invention two
The recovery rate of manage bar part, heparin sodium can achieve 22%, heparin sodium auxiliary extraction efficiency can be improved 6%.
Embodiment three
The power of supersonic generator 2 is 400W, ultrasonic frequency 40kHZ, and the external agency 15 in media Containers 5 is
20% calcium chloride solution, the conduction velocity of the ultrasonic wave in calcium chloride solution are 1531m/s, wavelength 3.8275cm, sample container
6 to amplitude transformer 4 distance be external agency 15 in ultrasonic frequency wavelength integral multiple, so that sample container 6 be arranged to luffing
The distance of bar 4 is 76.55cm；Interior media 16 is milk, and the conduction velocity of the ultrasonic wave in milk is 1473m/s, and wavelength is
3.6825cm, the internal diameter of sample container 6 is the integral multiple of ultrasonic frequency wavelength in interior media 16, so that sample container be arranged
6 internal diameter is 73.65cm；The material of sample container 6 is organic glass, and the conduction velocity of the ultrasonic wave in organic glass is
2692m/s, wavelength 6.73cm, the wall thickness of sample container 6 are less than ultrasonic frequency wavelength in 16 material therefor of interior media
1/10, so that the wall thickness that sample container 6 is arranged is less than 0.673cm.
For carrying out ultrasonic disruption to milk, using the ultrasonication condition in the embodiment of the present invention three, equal
Under conditions of matter 5 minutes, the average grain diameter of milk emulsion can be reduced to 1.0 microns or less.
Above-described is only preferred embodiments of the invention, and technical solution of the present invention is not limited thereto, it should be pointed out that
To those skilled in the art, under technical inspiration provided by the present invention, as the common knowledge of this field, also
Other equivalent modifications and improvement can be made, also should be regarded as protection scope of the present invention.
Claims (10)
1. a kind of ultrasonic cell-break device based on spherical wave resonance principle, including the ultrasound connected by transmission line (1)
Wave producer (2), energy converter (3) and amplitude transformer (4), it is characterised in that: the side of the supersonic generator (2) is equipped with medium
Container (5), the energy converter (3) are arranged between supersonic generator (2) and media Containers (5), the amplitude transformer (4) with change
Energy device (3) connects and is arranged in media Containers (5), is equipped with sample container (6) in the media Containers (5), the sample appearance
Device (6) is fixed in media Containers (5) by the fixed device of sample container.
2. a kind of ultrasonic cell-break device based on spherical wave resonance principle as described in claim 1, it is characterised in that:
The sample container (6) is spherical container, and the sample container (6) is equipped with feeder pipe (7), one end of the feeder pipe (7)
It is fixedly connected with sample container (6), the other end is extended on media Containers (5) outside.
3. a kind of ultrasonic cell-break device based on spherical wave resonance principle as claimed in claim 2, it is characterised in that:
The fixed device of the sample container includes fixed frame (8), connecting rod (9) and stop collar (10), the fixed frame (8) and Jie
The inner wall of matter container (5) is adapted, and the internal diameter of the stop collar (10) is adapted with the outer diameter of sample container (6), the fixation
Framework (8) is fixedly connected by connecting rod (9) with stop collar (10).
4. a kind of ultrasonic cell-break device based on spherical wave resonance principle as claimed in claim 3, it is characterised in that:
Compatible jacking block (11), the side of the jacking block (11) and sample container are equipped with sample container (6) in the connecting rod (9)
(6) it is in contact, the other side is connected with spring (12).
5. a kind of ultrasonic cell-break device based on spherical wave resonance principle as claimed in claim 4, it is characterised in that:
The interconnecting piece of the stop collar (10) and connecting rod (9) is equipped with sample container (6) compatible bottom gag lever post (13).
6. a kind of ultrasonic cell-break device as claimed in claim 5, it is characterised in that: the media Containers (5) it is interior
Wall is equipped with limited block (14).
7. a kind of ultrasonic cell-break device based on spherical wave resonance principle as claimed in claim 6, it is characterised in that:
External agency (15) are filled in the media Containers (5), are filled with interior media (16) in the sample container (6), it is described
The liquid of the tall and big spherical portion in sample container (6) of the liquid of external agency (15), the interior media (16) is tall and big in sample container
(6) the spherical portion and liquid for being less than external agency (15) is high.
8. a kind of ultrasonic cell-break device based on spherical wave resonance principle as claimed in claim 7, it is characterised in that:
The distance of the sample container (6) to amplitude transformer (4) is the integral multiple of ultrasonic frequency wavelength in external agency (15), the sample
The internal diameter of this container (6) is the integral multiple of ultrasonic frequency wavelength in interior media (16), and the wall thickness of the sample container (6) is small
The 1/10 of ultrasonic frequency wavelength in interior media (16) material therefor, the density of the external agency (15) are greater than internal be situated between
The density of matter (16).
9. a kind of ultrasonic cell-break device based on spherical wave resonance principle as claimed in claim 8, it is characterised in that:
The top of the media Containers (5) is equipped with top cover (17), and the top cover (17) passes through hinge (18) and media Containers (5) activity
Connection.
10. a kind of ultrasonic cell-break device based on spherical wave resonance principle as claimed in claim 9, feature exist
In: the top cover (17) be equipped with feeder pipe (7) can by through-hole (19), it is close to be socketed with feeder pipe on the through-hole (19)
The side plate of seal (20), the media Containers (5) is equipped with top seal pad (21) corresponding with top cover (17).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610782529.2A CN106434337B (en) | 2016-08-31 | 2016-08-31 | A kind of ultrasonic cell-break device based on spherical wave resonance principle |
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| CN201610782529.2A CN106434337B (en) | 2016-08-31 | 2016-08-31 | A kind of ultrasonic cell-break device based on spherical wave resonance principle |
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| CN106434337A CN106434337A (en) | 2017-02-22 |
| CN106434337B true CN106434337B (en) | 2019-03-12 |
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| CN112774533A (en) * | 2020-12-18 | 2021-05-11 | 昆山微电子技术研究院 | Solid-liquid dispersion device |
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|---|---|---|---|---|
| CN1102362A (en) * | 1993-10-07 | 1995-05-10 | 索菲马有限公司 | A method and a device for sonicating and transmitting vibrations to a sonication liquid containing particles |
| US5585044A (en) * | 1993-12-28 | 1996-12-17 | Hamamatsu Photonics K.K. | Liquid treating method and liquid treating apparatus |
| CN2581058Y (en) * | 2002-08-29 | 2003-10-22 | 程树群 | Emulsifying device for iodipin and medicinal preparation |
| CN101435064A (en) * | 2008-12-08 | 2009-05-20 | 清华大学 | High sound intensity ultrasonic processing apparatus for metal and alloy solidification and processing method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012125911A1 (en) * | 2011-03-17 | 2012-09-20 | Covaris, Inc. | Acoustic treatment vessel and method for acoustic treatment |
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2016
- 2016-08-31 CN CN201610782529.2A patent/CN106434337B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1102362A (en) * | 1993-10-07 | 1995-05-10 | 索菲马有限公司 | A method and a device for sonicating and transmitting vibrations to a sonication liquid containing particles |
| US5585044A (en) * | 1993-12-28 | 1996-12-17 | Hamamatsu Photonics K.K. | Liquid treating method and liquid treating apparatus |
| CN2581058Y (en) * | 2002-08-29 | 2003-10-22 | 程树群 | Emulsifying device for iodipin and medicinal preparation |
| CN101435064A (en) * | 2008-12-08 | 2009-05-20 | 清华大学 | High sound intensity ultrasonic processing apparatus for metal and alloy solidification and processing method thereof |
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