CN106433662A - Efficient soil remediator - Google Patents
Efficient soil remediator Download PDFInfo
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- CN106433662A CN106433662A CN201610735632.1A CN201610735632A CN106433662A CN 106433662 A CN106433662 A CN 106433662A CN 201610735632 A CN201610735632 A CN 201610735632A CN 106433662 A CN106433662 A CN 106433662A
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- soil
- enzymatic hydrolysate
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
Abstract
The invention provides a soil remediator which is short in remediation period, good in effect, lasting in action and environment-friendly. The soil remediator mainly comprises, by weight, 80-120 parts of pineapple leaf powder, 23-35 parts of sodium tripolyphosphate, 5-9 parts of proline, 4-8 parts of histidine, 6-11 parts of organic acid, 33-48 parts of enzymolysis product A, 12-19 parts of enzymolysis product B and 6-11 parts of vitamin C.
Description
Technical field
The present invention relates to technical field of soil remediation, and in particular to a kind of efficient soil-repairing agent.
Background technology
Heavy metal pollution of soil refers to due to mankind's activity, heavy metal is added in soil, causes heavy metal in soil to contain
Amount is apparently higher than its natural background content, and causes the phenomenon that ecological disruption and environmental quality deteriorate.At present, heavy metal pollution
The processing mode of soil mainly has engineering measure, chemical modifying, agronomic measures and 4 big class of biological restoration, but much processes
Mode is required for improving repairing effect using organic renovation agent.Heavy metal pollution of soil has disguised, chronicity and can not
Inverse property, holdup time of the pollutant in soil is long, and plant or microorganism can not be degraded, and heavy metal pollution does not only result in soil
The reduction of degeneration, crop yield and quality, and the life and health of the mankind may be jeopardized by directly contact, food chain.
Organic renovation agent is primarily referred to as a class compound of carbon containing, hydrogen, rise in the reparation of heavy metal pollution of soil complexation,
Dam, the effect of fixing heavy metal contaminants, and the organic renovation agent in part also has certain Detoxication to biological.With science and technology
Progress and the continuing to bring out of new technique, the new formulation for being applied to soil pollution reparation is on the increase, in soil environment protection
In played important function.But, only there are many drawbacks to soil remediation by simple organic renovation agent, for example, destroy soil
Earth structure causes the Biomass of crop to reduce, and causes soil element to be lost in；Difficult treatment, easily causes secondary pollution；There is biology
Toxicity, suppression plant growth the problems such as.
Content of the invention
In view of this, the present invention provides that a kind of repairing efficiency is short, and effect is good, environmentally friendly soil-repairing agent.
The technical scheme is that：A kind of efficient soil-repairing agent, the main component of the soil-repairing agent is by weight
Number meter includes：Pineapple leaves powder 80-120 part, sodium tripolyphosphate 23-35 part, proline 5-9 part, histidine 4-8 part is organic
Sour 6-11 part, enzymatic hydrolysate A 33-48 part, enzymatic hydrolysate B12-19 part, vitamin C 6-11 part.
Further, the main component of the soil-repairing agent count by weight including：98 parts of pineapple leaves powder, trimerization
25 parts of sodium phosphate, 7 parts of proline, 6 parts of histidine, 8 parts of organic acid, 41 parts of enzymatic hydrolysate A, 17 parts of enzymatic hydrolysate B, vitamin
8 parts of C.
Particularly, the organic acid be oxalic acid, malic acid, the combination of any one or more in citric acid.
In the present invention, sodium tripolyphosphate and organic acid have certain activation for the heavy metal in soil, more
Be conducive to the formation stable compound of the other components in renovation agent and heavy metal ion or its compound.Proline and histidine
Combination can heavy metal ion play good promotion chelation, promote enzymatic hydrolysate A, enzymatic hydrolysate B and heavy metal
Ion and its compound form stable complex, are adsorbed by arghan.
Further, the preparation method of the pineapple leaves powder is：S1. pineapple leaves are pressed 1:2 material-water ratio is in 1 mol
L-1, pH4.0 citrate buffer solution in soak 2-8h；S2. the pineapple leaves that soaked are standby after 80 DEG C of dryings；S3. will
Pineapple leaves are crushed using pulverizer, are crossed 20 mesh sieves, have both been obtained pineapple leaves powder.
Further, the enzymatic hydrolysate A is that pineapple bran enzymatic hydrolysate, its preparation method is：S1. pineapple bran is used
Blender is blended, by 1:20 amount adds water latter 65 DEG C and soaks 30min, filters to obtain filtering residue；S2. according to 1:2 solid-to-liquid ratio adds water,
Mix homogeneously, pretreatment 1min in the microwave of 600w power, add cellulase, enzyme dosage be-1, pH value
4.0,50 DEG C of hydrolysis temperature, response time 8h, reaction terminates enzyme denaturing 10min in the boiling water bath, obtains enzymolysis solution；S3. will be upper
Stating enzymolysis solution enzymatic hydrolysate A is obtained by being spray-dried.
Fructus Ananadis comosi also known as Fructus Ananadis comosi, China Guangdong, Guangxi, Hainan all have plants in area, and whole world Fructus Ananadis comosi yield in 2014 is
Break through 15,000,000 tons.Pineapple fruit not storage tolerance, after full maturity, shelf life is very short, mainly based on marketing fresh, Fructus Ananadis comosi
The product pineapple bran that has 50% -60% based on concentrated pineapple juice, canned pineapple whole slice in syrup etc., in process added
Factory discards, and these gurry parts are used as feedstuff, and a part is containing substantial amounts of fibre directly as garbage disposal, pineapple bran
Dimension, but the domestic research for utilizing for lacking to pineapple bran dietary fiber, this is not only the waste to living resources, and is right
Environment greatly pollutes.The present invention turns waste into wealth, resource is achieved fully using pineapple leaves and pineapple bran as raw material
Utilization, be that friendly environment society is made that contribution.
Further, the enzymatic hydrolysate B is wild mountain spinach enzymatic hydrolysate, and its preparation method is：S1. by wild Ulmus pumila L. spinach
Dish is dried to constant mass in 80 DEG C, crushes 20 mesh sieves, standby；S2. wild mountain spinach powder pre-treating, with concentration 0.5%
Sulphuric acid at 80 DEG C, by wild mountain spinach powder：Sulphuric acid=1:20 solid-to-liquid ratio stirring 30min, 5000rpm centrifugation 5-10min, takes
Precipitate；S3. digest, enzymatic hydrolysis condition is:Above-mentioned precipitate is pressed 1:3 solid-to-liquid ratio adds water, and adds cellulase, enzyme dosage
For 100U g-1, pH value 4.0,45 DEG C of hydrolysis temperature, response time 34h, reaction terminates enzyme denaturing in the boiling water bath
10min, obtains enzymolysis solution；S4. above-mentioned enzymolysis solution is obtained enzymatic hydrolysate B by being spray-dried.
It has been investigated that, wild mountain spinach can have adsorption for heavy metal element as a kind of super enriching plant.
Wild mountain spinach is digested its active component is obtained, more effective heavy metal ion is carried out chelating/complexing absorb,
Can further improve the efficiency of Metal uptake.In addition, in the present invention, vitamin C can be used as the small molecule thing of enzymatic hydrolysate B
The catalyst aid of matter and heavy metal chelating.Wild mountain spinach employed in the present invention is the whole strain of wild mountain spinach.
In the present invention, the pineapple leaves powder is to be combined closely to form by many fibre bundles, and each fibre bundle is again by 10-
20 individual fiber cells collection are combined into.Fiber surface is coarse, has longitudinal slot and hole, laterally has minor matters, no natural distortion.
By this special fibre structure, can by the metal composite active adsorption for chelating through renovation agent, be fixed on pineapple leaves fibre
In dimension, drastically reduce the heavy metal concentration in soil, and the microorganism in soil protection；Meanwhile, pineapple bran and wild Ulmus pumila L.
The enzymatic hydrolysate of Herba Spinaciae can be combined with the tunning of Soil Microorganism by being crosslinked complex role, macromolecular substances therein
High molecular polymer is formed in metal composite surface, this high molecular polymer can be to the metal composite in arghan
Have further and wrap pollutant.
The beneficial effects of the present invention is：(1) traditional organic renovation agent is different from, is lost in the metallic element in soil
Or most microorganisms are killed, soil-repairing agent prepared by the present invention, the metallic element in soil is stabilized in pineapple leaves
On fiber, drastically reduce the heavy metal concentration in soil, and the microorganism in soil protection；(2) containing organic in renovation agent
Acid and pineapple bran enzymatic hydrolysate, not only increase absorption, chelating/complexation energy of the arghan to heavy metal in soil ion
Power, simultaneously because containing multiple nutrition and active substance in pineapple bran enzymatic hydrolysate, increased the fertility of soil.(3) different
In traditional plant reparation, without the need for idle land；(4) raw material sources are wide, low production cost, achievable low value resource reasonable
Utilize；（5）The soil-repairing agent repairing efficiency of the present invention is short, and effect is good, and lasting effect is strong, is conducive to the popularization of correlation technique and answers
With.
Specific embodiment
With reference to embodiment, the invention will be further described, but protection scope of the present invention is not limited to this.
Embodiment 1
A kind of efficient soil-repairing agent, the main component of the soil-repairing agent count by weight including：Pineapple leaves powder 98
Part, 25 parts of sodium tripolyphosphate, 7 parts of proline, 6 parts of histidine, 8 parts of organic acid, 41 parts of enzymatic hydrolysate A, enzymatic hydrolysate B 17
Part, 8 parts of vitamin C.
Particularly, the organic acid is citric acid.
Further, the preparation method of the pineapple leaves powder is：S1. pineapple leaves are pressed 1:2 material-water ratio is in 1 mol
L-1, pH4.0 citrate buffer solution in soak 5h；S2. the pineapple leaves that soaked are standby after 80 DEG C of dryings；S3. by spinach
Luo Ye is crushed using pulverizer, is crossed 20 mesh sieves, had both been obtained pineapple leaves powder.
Further, the enzymatic hydrolysate A is that pineapple bran enzymatic hydrolysate, its preparation method is：S1. pineapple bran is used
Blender is blended, by 1:20 amount adds water latter 65 DEG C and soaks 30min, filters to obtain filtering residue；S2. according to 1:2 solid-to-liquid ratio adds water,
Mix homogeneously, pretreatment 1min in the microwave of 600w power, add cellulase, enzyme dosage be-1, pH value
4.0,50 DEG C of hydrolysis temperature, response time 8h, reaction terminates enzyme denaturing 10min in the boiling water bath, obtains enzymolysis solution；S3. will be upper
Stating enzymolysis solution enzymatic hydrolysate A is obtained by being spray-dried.
Further, the enzymatic hydrolysate B is wild mountain spinach enzymatic hydrolysate, and its preparation method is：S1. by wild Ulmus pumila L. spinach
Dish is dried to constant mass in 80 DEG C, crushes 20 mesh sieves, standby；S2. wild mountain spinach powder pre-treating, with concentration 0.5%
Sulphuric acid at 80 DEG C, by wild mountain spinach powder：Sulphuric acid=1:20 solid-to-liquid ratio stirring 30min, 5000rpm centrifugation 5-10min, takes
Precipitate；S3. digest, enzymatic hydrolysis condition is:Above-mentioned precipitate is pressed 1:3 solid-to-liquid ratio adds water, and adds cellulase, enzyme dosage
For 100U g-1, pH value 4.0,45 DEG C of hydrolysis temperature, response time 34h, reaction terminates enzyme denaturing in the boiling water bath
10min, obtains enzymolysis solution；S4. above-mentioned enzymolysis solution is obtained enzymatic hydrolysate B by being spray-dried.
Embodiment 2
A kind of efficient soil-repairing agent, the main component of the soil-repairing agent count by weight including：Pineapple leaves powder 80
Part, 25 parts of sodium tripolyphosphate, 7 parts of proline, 6 parts of histidine, 8 parts of organic acid, 48 parts of enzymatic hydrolysate A, enzymatic hydrolysate B 12
Part, 8 parts of vitamin C.
Particularly, the organic acid is oxalic acid and citric acid 1：1 mixed acid.
Further, the preparation method of the pineapple leaves powder is：S1. pineapple leaves are pressed 1:2 material-water ratio is in 1 mol
L-1, pH4.0 citrate buffer solution in soak 5 h；S2. the pineapple leaves that soaked are standby after 80 DEG C of dryings；S3. will
Pineapple leaves are crushed using pulverizer, are crossed 20 mesh sieves, have both been obtained pineapple leaves powder.
Further, the enzymatic hydrolysate A is that pineapple bran enzymatic hydrolysate, its preparation method is：S1. pineapple bran is used
Blender is blended, by 1:20 amount adds water latter 65 DEG C and soaks 30min, filters to obtain filtering residue；S2. according to 1:2 solid-to-liquid ratio adds water,
Mix homogeneously, pretreatment 1min in the microwave of 600w power, add cellulase, enzyme dosage be-1, pH value
4.0,50 DEG C of hydrolysis temperature, response time 8h, reaction terminates enzyme denaturing 10min in the boiling water bath, obtains enzymolysis solution；S3. will be upper
Stating enzymolysis solution enzymatic hydrolysate A is obtained by being spray-dried.
Further, the enzymatic hydrolysate B is wild mountain spinach enzymatic hydrolysate, and its preparation method is：S1. by wild Ulmus pumila L. spinach
Dish is dried to constant mass in 80 DEG C, crushes 20 mesh sieves, standby；S2. wild mountain spinach powder pre-treating, with concentration 0.5%
Sulphuric acid at 80 DEG C, by wild mountain spinach powder：Sulphuric acid=1:20 solid-to-liquid ratio stirring 30min, 5000rpm centrifugation 5-10min, takes
Precipitate；S3. digest, enzymatic hydrolysis condition is:Above-mentioned precipitate is pressed 1:3 solid-to-liquid ratio adds water, and adds cellulase, enzyme dosage
For 100U g-1, pH value 4.0,45 DEG C of hydrolysis temperature, response time 34h, reaction terminates enzyme denaturing in the boiling water bath
10min, obtains enzymolysis solution；S4. above-mentioned enzymolysis solution is obtained enzymatic hydrolysate B by being spray-dried.
Embodiment 3
A kind of efficient soil-repairing agent, the main component of the soil-repairing agent count by weight including：Pineapple leaves powder 120
Part, 25 parts of sodium tripolyphosphate, 7 parts of proline, 6 parts of histidine, 8 parts of organic acid, 33 parts of enzymatic hydrolysate A, enzymatic hydrolysate B 19
Part, 8 parts of vitamin C.
Particularly, the organic acid is oxalic acid.
Further, the preparation method of the pineapple leaves powder is：S1. pineapple leaves are pressed 1:2 material-water ratio is in 1 mol
L-1, pH4.0 citrate buffer solution in soak 8h；S2. the pineapple leaves that soaked are standby after 80 DEG C of dryings；S3. by spinach
Luo Ye is crushed using pulverizer, is crossed 20 mesh sieves, had both been obtained pineapple leaves powder.
Further, the enzymatic hydrolysate A is that pineapple bran enzymatic hydrolysate, its preparation method is：S1. pineapple bran is used
Blender is blended, by 1:20 amount adds water latter 65 DEG C and soaks 30min, filters to obtain filtering residue；S2. according to 1:2 solid-to-liquid ratio adds water,
Mix homogeneously, pretreatment 1min in the microwave of 600w power, add cellulase, enzyme dosage be-1, pH value
4.0,50 DEG C of hydrolysis temperature, response time 8h, reaction terminates enzyme denaturing 10min in the boiling water bath, obtains enzymolysis solution；S3. will be upper
Stating enzymolysis solution enzymatic hydrolysate A is obtained by being spray-dried.
Further, the enzymatic hydrolysate B is wild mountain spinach enzymatic hydrolysate, and its preparation method is：S1. by wild Ulmus pumila L. spinach
Dish is dried to constant mass in 80 DEG C, crushes 20 mesh sieves, standby；S2. wild mountain spinach powder pre-treating, with concentration 0.5%
Sulphuric acid at 80 DEG C, by wild mountain spinach powder：Sulphuric acid=1:20 solid-to-liquid ratio stirring 30min, 5000rpm centrifugation 5-10min, takes
Precipitate；S3. digest, enzymatic hydrolysis condition is:Above-mentioned precipitate is pressed 1:3 solid-to-liquid ratio adds water, and adds cellulase, enzyme dosage
For 100U g-1, pH value 4.0,45 DEG C of hydrolysis temperature, response time 34h, reaction terminates enzyme denaturing in the boiling water bath
10min, obtains enzymolysis solution；S4. above-mentioned enzymolysis solution is obtained enzymatic hydrolysate B by being spray-dried.
Comparative example 1
A kind of efficient soil-repairing agent, the main component of the soil-repairing agent count by weight including：Pineapple leaves powder 98
Part, 25 parts of sodium tripolyphosphate, 7 parts of proline, 6 parts of histidine, 8 parts of organic acid, 17 parts of enzymatic hydrolysate B, 8 parts of vitamin C.
Particularly, the organic acid is citric acid.
Further, the preparation method of the pineapple leaves powder is：S1. pineapple leaves are pressed 1:2 material-water ratio is in 1 mol
L-1, pH4.0 citrate buffer solution in soak 5h；S2. the pineapple leaves that soaked are standby after 80 DEG C of dryings；S3. by spinach
Luo Ye is crushed using pulverizer, is crossed 20 mesh sieves, had both been obtained pineapple leaves powder.
Further, the enzymatic hydrolysate B is wild mountain spinach enzymatic hydrolysate, and its preparation method is：S1. by wild Ulmus pumila L. spinach
Dish is dried to constant mass in 80 DEG C, crushes 20 mesh sieves, standby；S2. wild mountain spinach powder pre-treating, with concentration 0.5%
Sulphuric acid at 80 DEG C, by wild mountain spinach powder：Sulphuric acid=1:20 solid-to-liquid ratio stirring 30min, 5000rpm centrifugation 5-10min, takes
Precipitate；S3. digest, enzymatic hydrolysis condition is:Above-mentioned precipitate is pressed 1:3 solid-to-liquid ratio adds water, and adds cellulase, enzyme dosage
For 100U g-1, pH value 4.0,45 DEG C of hydrolysis temperature, response time 34h, reaction terminates enzyme denaturing in the boiling water bath
10min, obtains enzymolysis solution；S4. above-mentioned enzymolysis solution is obtained enzymatic hydrolysate B by being spray-dried.
Comparative example 2
A kind of efficient soil-repairing agent, the main component of the soil-repairing agent count by weight including：Pineapple leaves powder 98
Part, 25 parts of sodium tripolyphosphate, 7 parts of proline, 6 parts of histidine, 8 parts of organic acid, 41 parts of enzymatic hydrolysate A, 8 parts of vitamin C.
Particularly, the organic acid is malic acid.
Further, the preparation method of the pineapple leaves powder is：S1. pineapple leaves are pressed 1:2 material-water ratio is in 1 mol
L-1, pH4.0 citrate buffer solution in soak 5h；S2. the pineapple leaves that soaked are standby after 80 DEG C of dryings；S3. by spinach
Luo Ye is crushed using pulverizer, is crossed 20 mesh sieves, had both been obtained pineapple leaves powder.
Further, the enzymatic hydrolysate A is that pineapple bran enzymatic hydrolysate, its preparation method is：S1. pineapple bran is used
Blender is blended, by 1:20 amount adds water latter 65 DEG C and soaks 30min, filters to obtain filtering residue；S2. according to 1:2 solid-to-liquid ratio adds water,
Mix homogeneously, pretreatment 1min in the microwave of 600w power, add cellulase, enzyme dosage be-1, pH value
4.0,50 DEG C of hydrolysis temperature, response time 8h, reaction terminates enzyme denaturing 10min in the boiling water bath, obtains enzymolysis solution；S3. will be upper
Stating enzymolysis solution enzymatic hydrolysate A is obtained by being spray-dried.
Implementation result is tested
Test case 1
By the soil-repairing agent in 12g embodiment 1-3 and comparative example 1-2 and 500g heavy metal (Mn2+、Mn4+、As3+) contain
The contaminated soil mixing for 1% is measured, is added 800mL water, be stirred at room temperature 2 hours, standing, heavy metal ion in test solution
Content, calculates removal of heavy metal ions rate as shown in the table.
Test case 2
With reference to GB/T10586-2006 moisture-heat test-box technical conditions, GB/T10592-2008 high-low temperature test chamber technical conditions with
And the standard of GB/T11158-2008 high-temperature test chamber technical conditions, using one kind that China Patent No. is 201210303557.3
Cladding ability of the combined environment test device for renovation agent of the present invention for heavy metal carries out environmental test test, to using survey
In examination example 1, the soil of embodiment 1-3 that method was processed carries out environmental testing, the environmental condition equivalence with the simulation of this environmental testing
Outdoor conditions for temperature be 35 DEG C, relative humidity be 85%, intensity of illumination be 50000Lux, with the simulation of this environmental testing
The elapsed-time standards change of environmental condition equivalence is turned to 0.5,1,3,5 years, after experiment terminates, by the soil to embodiment 1-3
The test of content of beary metal, in binding test example 1 in embodiment 1-3 heavy metal-polluted soil clearance, calculate heavy metal release
Rate is to verify cladding ability of the renovation agent for heavy metal, as a result as shown in the table.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, and do not carrying on the back
In the case of the spirit or essential attributes of the present invention, the present invention can be realized in other specific forms.Therefore, no matter from which
From the point of view of a bit, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention will by appended right
Ask rather than described above is limited, it is intended that all changes that will fall in the implication and scope of the equivalency of claim
Include in the present invention.
Moreover, it will be appreciated that although this specification is been described by according to embodiment, not each embodiment only includes one
Individual independent technical scheme, this narrating mode of description is only that those skilled in the art will should say for clarity
Bright book can also form those skilled in the art permissible through appropriately combined as an entirety, the technical scheme in each embodiment
The other embodiment of understanding.For the ins and outs of all not detailed descriptions in the present invention, all this area can be passed through arbitrary existing
Technology is had to realize.
Claims (5)
1. a kind of efficient soil-repairing agent, it is characterised in that：The main component of the soil-repairing agent count by weight including：
Pineapple leaves powder 80-120 part, sodium tripolyphosphate 23-35 part, proline 5-9 part, histidine 4-8 part, organic acid 6-11 part, enzyme
Solution product A 33-48 part, enzymatic hydrolysate B12-19 part, vitamin C 6-11 part.
2. soil-repairing agent according to claim 1, it is characterised in that the main component of the soil-repairing agent is by weight
Number meter includes：98 parts of pineapple leaves powder, 25 parts of sodium tripolyphosphate, 7 parts of proline, 6 parts of histidine, 8 parts of organic acid, enzymolysis is produced
17 parts of 41 parts of thing A, enzymatic hydrolysate B, 8 parts of vitamin C.
3. soil-repairing agent according to claim 1, it is characterised in that the preparation method of the pineapple leaves powder is：S1.
Pineapple leaves are pressed 1:2 material-water ratio is in 1 mol L-1, pH4.0 citrate buffer solution in soak 2-8h；S2. will soak
Pineapple leaves standby after 80 DEG C of dryings；S3. pineapple leaves are crushed using pulverizer, 20 mesh sieves is crossed, had both obtained pineapple leaves
Powder.
4. soil-repairing agent according to claim 1, it is characterised in that the enzymatic hydrolysate A is that pineapple bran enzymolysis is produced
Thing, its preparation method is：S1. pineapple bran is blended with blender, by 1:20 amount adds water latter 65 DEG C and soaks 30min, filters
Obtain filtering residue；S2. according to 1:2 solid-to-liquid ratio adds water, mix homogeneously, pretreatment 1min in the microwave of 600w power, adds fiber
Plain enzyme, enzyme dosage is 600U g-1, pH value 4.0,50 DEG C of hydrolysis temperature, response time 8h, reaction terminates after boiling water bath
Middle enzyme denaturing 10min, obtains enzymolysis solution；S3. above-mentioned enzymolysis solution is obtained enzymatic hydrolysate A by being spray-dried.
5. soil-repairing agent according to claim 1, it is characterised in that the enzymatic hydrolysate B is wild mountain spinach enzymolysis
Product, its preparation method is：S1. wild mountain spinach is dried to constant mass in 80 DEG C, 20 mesh sieves was crushed, standby；S2. wild
Mountain spinach powder pre-treating, with the sulphuric acid of concentration 0.5% at 80 DEG C, by wild mountain spinach powder：Sulphuric acid=1:20 solid-to-liquid ratio
Stirring 30min, 5000rpm centrifugation 5-10min, taking precipitate；S3. digest, enzymatic hydrolysis condition is:Above-mentioned precipitate is pressed 1:3
Solid-to-liquid ratio adds water, and adds cellulase, and enzyme dosage is 100U g-1, pH value 4.0,45 DEG C of hydrolysis temperature, the response time
34h, reaction terminates enzyme denaturing 10min in the boiling water bath, obtains enzymolysis solution；S4. above-mentioned enzymolysis solution is obtained by being spray-dried
Enzymatic hydrolysate B.
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| CN201610735632.1A CN106433662A (en) | 2016-08-26 | 2016-08-26 | Efficient soil remediator |
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| CN201610735632.1A CN106433662A (en) | 2016-08-26 | 2016-08-26 | Efficient soil remediator |
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Family
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107118780A (en) * | 2017-07-05 | 2017-09-01 | 青岛农业大学 | It is a kind of based on biomass carbon and residual grey soil-repairing agent and preparation method thereof |
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| CN103468269A (en) * | 2013-08-21 | 2013-12-25 | 太仓碧奇新材料研发有限公司 | Preparation method of material for restoring soil polluted by heavy metal |
| CN105567248A (en) * | 2016-02-04 | 2016-05-11 | 周益辉 | Soil heavy metal passivator and preparation method and application thereof |
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2016
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| CN103468269A (en) * | 2013-08-21 | 2013-12-25 | 太仓碧奇新材料研发有限公司 | Preparation method of material for restoring soil polluted by heavy metal |
| CN105567248A (en) * | 2016-02-04 | 2016-05-11 | 周益辉 | Soil heavy metal passivator and preparation method and application thereof |
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