CN106418110A - Natural antibacterial agent used in meat-flavored essence and preparation method thereof - Google Patents

Natural antibacterial agent used in meat-flavored essence and preparation method thereof Download PDF

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Publication number
CN106418110A
CN106418110A CN201610875424.1A CN201610875424A CN106418110A CN 106418110 A CN106418110 A CN 106418110A CN 201610875424 A CN201610875424 A CN 201610875424A CN 106418110 A CN106418110 A CN 106418110A
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CN
China
Prior art keywords
antibacterial
natural
preparation
bacteriostatic agent
natamycin
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610875424.1A
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Chinese (zh)
Inventor
祖庆勇
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Ningxia Spring Source Biotechnology Co Ltd
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Ningxia Spring Source Biotechnology Co Ltd
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Publication date
Application filed by Ningxia Spring Source Biotechnology Co Ltd filed Critical Ningxia Spring Source Biotechnology Co Ltd
Priority to CN201610875424.1A priority Critical patent/CN106418110A/en
Publication of CN106418110A publication Critical patent/CN106418110A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463Organic compounds; Microorganisms; Enzymes
    • A23L3/34635Antibiotics
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463Organic compounds; Microorganisms; Enzymes
    • A23L3/3562Sugars; Derivatives thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)

Abstract

The invention relates to a natural antibacterial agent used in meat-flavored essence and a preparation method thereof. The antibacterial agent is prepared from, by weight, 12.0-30.0% of maltodextrin, 1.0%-2.0% of emulsifier, 3.0-5.0% of Arabicgum, 10.0-15.0% of lactose, 15.0%-30.0% of mixed bacteriostatic agent and 40.0-70.0% of water, wherein the sum of the weight percentages of the components is 100%, the mixed bacteriostatic agent is the mixture of an antibacterial peptide, nisin and natamycin, and the mass ratio of the antibacterial peptide to the nisin to the natamycin is 1-3 to 3-5 to 5-7. The used antibacterial peptide, nisin and natamycin are natural ingredients. The natural antibacterial agent can be widely used in natural meat-flavored essence products and plays the best antibacterial and antiseptic effects inappropriate proportion.

Description

A kind of for natural bacteriostatic agent in meat flavor essence and preparation method thereof
Technical field
The invention belongs to food processing field, it is related to a kind of antibacterial, particularly to a kind of for the suppression in meat flavor essence Microbial inoculum and preparation method thereof.
Background technology
Along with the fast development of food and catering industry, driven the fast development of meat flavor essence, at present for natural Meat flavor essence have become as the reason a kind of irresistible trend, this behind be exactly people food quality is had higher will Ask, food enterprise more modernizes, catering industry starts Chain-orientation and scale, all can promote meat flavor essence towards high-quality side To development.But it is necessary for here paying close attention to the Anticorrosion of natural meat flavour essence, because the essence preservative of natural class is necessary for It is also natural.
The mankind in ancient times just have very deep understanding it was found that the method that much makes the food storage phase extend, example to anti-corrosion As:Pickle, processed with honey, sootiness or deposit in underground, water.Traditional anti-corrosion method can not meet the industrialization of modern food Demand.People use upper more efficient and convenient, low cost, nontoxic to the requirement for anticorrosion of food.Avoid using chemistry as far as possible The preservative of product.The chemical preservation of early stage mainly has the high poison product such as formaldehyde, Nitrateses, investigated formic acid, benzene afterwards again The tens of kinds of chemical preservatives such as sodium formate, sodium dehydroacetate, sodium diacetate.Food preservative is made to the development of modern food industry Go out very big contribution.But contain the chemical composition of excessive synthetic in preservative, health can be produced unavoidably negative Face rings.
With the development of industry and the demand of down-stream enterprise, natural preservative is inevitable trend.So food service industry Middle exploitation natural antisepsis fresh-keeping agent will be an important task.Common natural antiseptic agent mainly has antibacterial peptide, breast on the market Acid streptococci element, natamycin, tea polyphenols, rutin, shitosan, epsilon-polylysine, water-soluble chitosan oligosaccharide etc. by food row Industry is adopted, but these situations about being all single use.The effective ingredient of each natural antiseptic agent and its mechanism of action are not With, have only has inhibition to specified germ.So being difficult to play overall antiseptical effect.The microorganism of so product refers to Mark always occurs underproof phenomenon, eventually results in the economy of enterprise and social sanction's degree all can incur loss.
Content of the invention
In view of current natural meat flavour essence is in the booming stage, occur that some natural meat flavour essences are corrupt unavoidably Problem, while chemical food preservations action effect is obvious, again can on health produce impact the problems such as.Mesh of the present invention Be to provide a kind of natural bacteriostatic agent and preparation method thereof, simple to operate and also there is very strong antiseptic and inhibiting bacteria function effect, from And the shelf-life of meat flavor essence can be extended.
The technical solution used in the present invention is:
A kind of for the natural bacteriostatic agent in meat flavor essence, this antibacterial includes the component of following percentage by weight:Fructus Hordei Germinatus Dextrin is 12.0-30.0%, emulsifying agent is 1.0%-2.0%, arabic gum is 3.0-5.0%, Lactose is 10.0-15.0%, Mixing antibacterial accounts for 15.0%-30.0%;Water accounts for 40.0-70.0%;The percentage by weight sum of described component is 100%;Its Described in mixing antibacterial be antibacterial peptide, nisin, the combination of natamycin, and the mass ratio of three is:Antibacterial Peptide:Nisin:Natamycin=1-3:3-5:5-7.
Present invention also offers the preparation method of above-mentioned natural bacteriostatic agent, comprise the steps:By the mixing of described component all Even, with high-speed emulsifying homogeneous machine in 10000~15000rpm condition down cut 3-5min, prepare emulsion;Then spray Drying prepares powder natural bacteriostatic agent.
Preferably, the inlet temperature of described spray drying is 160~180 DEG C, and leaving air temp is 70~80 DEG C, charging rate For 5~10mL/min.
The present invention is had the advantage that:
Antibacterial peptide used in the present invention, nisin, natamycin, are all natural components.Can widely answer On natural meat flavour essence product, and under suitable proportioning, play optimal antibacterial, antiseptical effect.Fragrant for food The meat flavor essence product of smart enterprise brings safe, green, healthy guarantee.
Specific embodiment
With reference to specific embodiment, the invention will be further described, but does not limit protection scope of the present invention.
Embodiment 1
A kind of natural bacteriostatic agent for meat flavor essence, this antibacterial includes the component of following percentage by weight:
Maltodextrin is 15.0%, emulsifying agent is 1.5%, arabic gum is 3.0%, Lactose is 10.0%, and mixing is antibacterial Agent (antibacterial peptide:Nisin:Natamycin=1:3:5) account for 25.5%;Water accounts for 45.0%.
The preparation method of this natural bacteriostatic agent, comprises the steps:By above-mentioned material mix homogeneously, use high-speed emulsifying homogeneous Machine, in 15000rpm condition down cut 5min, obtains emulsion;Finally it is spray-dried and prepare powder natural bacteriostatic agent.Institute The inlet temperature stating spray drying is 180 DEG C, and leaving air temp is 80 DEG C, and charging rate is 8mL/min.
Fungistatic effect is tested
Separate from natural meat flavour essence, purification obtains lactobacilluss and enterobacteria, cultivated with culture medium respectively.
Fungistatic effect is passed judgment on using filter paper enzyme:1. with card punch, the filter paper of thickness 1.5mm is broken into diameter 5mm disk, Parcel is after 160 DEG C of dry heat sterilization 2h.2. flat board preparation:It is used for each Selective agar medium heating and melting for examination bacterium by prepare, It is poured in the culture dish of dry heat sterilization, standby after flat board solidification.3. bacteria suspension preparation:The bacterium picking of culture after activation Add in 9mL physiological saline solution.Mix 1min with vortex mixed instrument.Become uniform bacteria suspension.4. aseptic absorption 0.2mL is each For examination bacteria suspension in plate, coating is uniformly.5. filter paper is soaked respectively in the antibacterial liquid of the variable concentrations preparing in advance Bubble 1min, takes out and is gently affixed on flat board, every plate uniformly puts 7 filter papers.6. plate is placed in 30 DEG C in constant incubator Culture 48h.Measure each inhibition zone size with micrometer.
Gained antibacterial of the present invention does antibacterial reality by the microorganism with the antibacterial isolated in meat flavor essence and Mycophyta Test.Compare with single antibacterial, measure each inhibition zone size, find that compound preservative effect is fairly obvious, can press down completely Make putrefaction bacteria.

Claims (3)

1. a kind of for the natural bacteriostatic agent in meat flavor essence it is characterised in that:This antibacterial includes following percentage by weight Component:Maltodextrin is 12.0-30.0%, emulsifying agent is 1.0%-2.0%, arabic gum is 3.0-5.0%, Lactose is 10.0-15.0%, mixing antibacterial accounts for 15.0%-30.0%;Water accounts for 40.0-70.0%;The percentage by weight of described component it With for 100%;Wherein said mixing antibacterial is antibacterial peptide, nisin, the combination of natamycin, and the matter of three Measuring ratio is:Antibacterial peptide:Nisin:Natamycin=1-3:3-5:5-7.
2. the natural bacteriostatic agent described in claim 1 preparation method it is characterised in that:Comprise the steps:By described component Mix homogeneously, with high-speed emulsifying homogeneous machine in 10000~15000rpm condition down cut 3-5min, prepares emulsion;So It is spray-dried afterwards and prepare powder natural bacteriostatic agent.
3. natural bacteriostatic agent according to claim 2 preparation method it is characterised in that:Described spray drying enter pathogenic wind-warm Spend for 160~180 DEG C, leaving air temp is 70~80 DEG C, charging rate is 5~10mL/min.
CN201610875424.1A 2016-10-08 2016-10-08 Natural antibacterial agent used in meat-flavored essence and preparation method thereof Pending CN106418110A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610875424.1A CN106418110A (en) 2016-10-08 2016-10-08 Natural antibacterial agent used in meat-flavored essence and preparation method thereof

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Application Number Priority Date Filing Date Title
CN201610875424.1A CN106418110A (en) 2016-10-08 2016-10-08 Natural antibacterial agent used in meat-flavored essence and preparation method thereof

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Publication Number Publication Date
CN106418110A true CN106418110A (en) 2017-02-22

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101715811A (en) * 2009-11-13 2010-06-02 国家农产品保鲜工程技术研究中心(天津) Nano biological fruits and vegetables coating agent and preparation method thereof
CN103040718A (en) * 2012-12-20 2013-04-17 广州栋方日化有限公司 Anti-corrosion composition, preparation method thereof and cosmetic
CN103355730A (en) * 2013-08-06 2013-10-23 山东省蓝源生物工程有限公司 Nisin composite biological preservative and preparation method thereof
CN104472646A (en) * 2014-11-10 2015-04-01 天津科技大学 Preparation method of Nisin double layer microcapsule suitable for high temperature baking food
CN104491837A (en) * 2014-10-30 2015-04-08 广东海纳川药业股份有限公司 Antibacterial peptide preparation, and preparation method and application thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101715811A (en) * 2009-11-13 2010-06-02 国家农产品保鲜工程技术研究中心(天津) Nano biological fruits and vegetables coating agent and preparation method thereof
CN103040718A (en) * 2012-12-20 2013-04-17 广州栋方日化有限公司 Anti-corrosion composition, preparation method thereof and cosmetic
CN103355730A (en) * 2013-08-06 2013-10-23 山东省蓝源生物工程有限公司 Nisin composite biological preservative and preparation method thereof
CN104491837A (en) * 2014-10-30 2015-04-08 广东海纳川药业股份有限公司 Antibacterial peptide preparation, and preparation method and application thereof
CN104472646A (en) * 2014-11-10 2015-04-01 天津科技大学 Preparation method of Nisin double layer microcapsule suitable for high temperature baking food

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Application publication date: 20170222