CN106405105A - Chemiluminescence enzyme-linked immunoassay kit of human epididymis protein - Google Patents
Chemiluminescence enzyme-linked immunoassay kit of human epididymis protein Download PDFInfo
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- CN106405105A CN106405105A CN201610798643.4A CN201610798643A CN106405105A CN 106405105 A CN106405105 A CN 106405105A CN 201610798643 A CN201610798643 A CN 201610798643A CN 106405105 A CN106405105 A CN 106405105A
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- solution
- chemiluminescence
- liquid
- enzyme
- phosphate buffer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57449—Specifically defined cancers of ovaries
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- Urology & Nephrology (AREA)
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a chemiluminescence enzyme-linked immunoassay kit of a human epididymis protein. The chemiluminescence enzyme-linked immunoassay kit is characterized by comprising a kit body, a marker arranged in the kit body and a reagent arranged in the kit body, wherein the marker is selected from a radioactive substance, a chemiluminescence or color developing material, biotin, metal horseshoe crab, fluorescein, an enzyme and a nano material; the reagent is prepared from: a hybridization solution, a synergist, a color developing agent, a sample diluting solution, streptavidin-HRP (Hypothalamic Regulatory Peptides), a chemiluminescence solution A and a concentrated washing solution, wherein the sample diluting solution is a phosphate buffer solution with the concentration of 0.05mol/L and has the pH (Potential of Hydrogen) value of 7.0; the phosphate buffer solution is a water solution which contains 16g of NaCl, 0.8g of KCl, 0.8g of KH2PO4 and 6.4g of Na2HPO4 in each liter; the chemiluminescence solution A is a tris(hydroxymethyl)metyl aminomethane solution with the content of luminol of 0.01M, the content of p-cresol of 0.001M and the pH value of 8.8; the concentrated washing solution is a phosphate buffer solution which contains Tween-20 with the volume percent of 0.05 percent, has the pH value of 7.4 and has the concentration of 0.1mol/L.
Description
Technical field
The present invention relates to a kind of test kit, particularly design a kind of chemiluminescent enzyme-linked immunosorbent immune detection examination of people's epididymal proteins
Agent box.
Background technology
Ovarian cancer is one of common tumor of female sex organ, and sickness rate is only second to cervical cancer and carcinoma of uterine body and arranges
Occupy the 3rd.But because ovarian cancer causes the dead, but account for the first place of all kinds of gynecological tumors, women's life is caused seriously to threaten.Due to
The more complicated tumor that it is suffered from of the fetal development of ovary, anatomic tissue and endocrine function is probably optimum or pernicious.Because of ovary
Cancer clinical early stage is asymptomatic, differentiates its organization type and good pernicious extremely difficult, its incidence of occult, during about 75% patient assessment
It has been middle and advanced stage, prognosis is poor, and its case fatality rate occupies first of gynecologic malignant tumor.Due to lacking specific clinical symptoms, only
19% ovarian cancer patients are to be diagnosed before Invasion of cancer and effectively treatment.Although the controlling of ovarian cancer between past 20 years
Treatment means achieve progress, are but not apparent from improving the cure rate of ovarian cancer, find tumor limitation in ovarian cancer row exploratory laparotomy
Only account for 30% in ovary, great majority have spread to uterus bilateral adnexa, omentum majuss and each organ of pelvic cavity, so ovarian cancer is no matter
Really a great problem in diagnosis and treatment, the pathomorphism to malignant tumor of ovary for the brainstrust for many years, clinic develops
Rule and therapeutic scheme have carried out many discussions, have accumulated substantial amounts of experience up to the present, with regard to domestic and international clinical data system
Meter, its five year survival rate only 25%～30%, main cause is to find in late period, treatment of late stage.In view of ovarian cancer early stage and evening
Phase treats the difference of prognosis, and finding one can have important clinical meaning in the diagnostic method of early discovery ovarian cancer, special
Be not pre-cancerous screening marker clinical meaning bigger, thus may accomplish preventative early screening and treatment, reduce
The M ＆ M of ovarian cancer.
Content of the invention
In order to solve the problems of the prior art, the invention provides a kind of chemiluminescence enzyme of people's epididymal proteins (HE4)
Linked immune assay kit.
In order to solve the above problems, the technical solution used in the present invention is：
A kind of chemical luminescence ELISA detection kit of people's epididymal proteins it is characterised in that：Including box body, it is located at box
Internal label and the reagent being located in box body, described label is selected from radioactive substance, chemiluminescence or substance that show color, life
Thing element, metal king crab, fluorescein, enzyme and nano material, described reagent includes：Hybridization solution, synergist, developer, Sample dilution,
Streptavidin-HRP, chemical luminescence for liquid, liquid, concentrated cleaning solution, described Sample dilution is 0.05mol/L phosphate-buffered
Liquid, pH=7.0, phosphate buffer is every liter and contains 16g NaCl, 0.8g KCl, 0.8g KH2PO4, 6.4g Na2HPO4's
Aqueous solution, described chemical luminescence for liquid A liquid for luminol content be 0.01M, p-cresol content be 0.001M pH=8.8 three
Hydroxymethyl aminomethane solution, described concentrated cleaning solution is the pH=7.4 containing volume fraction 0.05% tween 20,0.1mol/
L phosphate buffer.
The beneficial effect that the present invention is reached：Test kit of the present invention utilizes the base of antigen and the specific immune response of antibody
Present principles are realizing.Chemiluminescence immunoassay is the product that chemoluminescence method and immunoassay combine, therefore simultaneously
There is the high sensitivity of chemoluminescence method and the high specific of immunoassay.In whole course of reaction, people's epididymis in sample
Protein content is higher, and in reaction system, luminous intensity is stronger；Conversely, people's epididymal proteins content is fewer in sample, luminous intensity is got over
Weak.The chemical luminescence ELISA detection kit of the present invention has the characteristics that high sensitivity, easy quick, accuracy are high, with
Traditional ELISA method compares, and the operating time is greatly reduced.Can be used as detecting the auxiliary diagnosis of hepatocarcinoma.
Specific embodiment
Following examples are only used for clearly technical scheme being described, and can not limit the present invention with this
Protection domain.
A kind of chemical luminescence ELISA detection kit of people's epididymal proteins it is characterised in that：Including box body, it is located at box
Internal label and the reagent being located in box body, described label is selected from radioactive substance, chemiluminescence or substance that show color, life
Thing element, metal king crab, fluorescein, enzyme and nano material, described reagent includes：Hybridization solution, synergist, developer, Sample dilution,
Streptavidin-HRP, chemical luminescence for liquid, liquid, concentrated cleaning solution, described Sample dilution is 0.05mol/L phosphate-buffered
Liquid, pH=7.0, phosphate buffer is every liter and contains 16g NaCl, 0.8g KCl, 0.8g KH2PO4, 6.4g Na2HPO4's
Aqueous solution, described chemical luminescence for liquid A liquid for luminol content be 0.01M, p-cresol content be 0.001M pH=8.8 three
Hydroxymethyl aminomethane solution, described concentrated cleaning solution is the pH=7.4 containing volume fraction 0.05% tween 20,0.1mol/
L phosphate buffer.
Test kit of the present invention is realized using the ultimate principle of antigen and the specific immune response of antibody.Chemiluminescence
Immunoassay is the product that chemoluminescence method and immunoassay combine, and therefore has the high sensitivity of chemoluminescence method simultaneously
High specific with immunoassay.In whole course of reaction, in sample, people's epididymal proteins content is higher, sends out in reaction system
Light intensity is stronger；Conversely, people's epididymal proteins content is fewer in sample, luminous intensity is weaker.The chemiluminescent enzyme-linked immunosorbent of the present invention is exempted from
Epidemic disease detection kit has the characteristics that high sensitivity, easy quick, accuracy are high, compares with traditional ELISA method, the operating time
It is greatly reduced.Can be used as detecting the auxiliary diagnosis of hepatocarcinoma.
Ultimate principle, principal character and the advantage of the present invention have been shown and described above.The technical staff of the industry should
Understand, the present invention is not restricted to the described embodiments, the simply explanation present invention's described in above-described embodiment and description is former
Reason, without departing from the spirit and scope of the present invention, the present invention also has various changes and modifications, these changes and improvements
Both fall within scope of the claimed invention.Claimed scope is by appending claims and its equivalent circle.
Fixed.
Claims (1)
1. a kind of chemical luminescence ELISA detection kit of people's epididymal proteins it is characterised in that：Including box body, it is located at box body
Interior label and the reagent being located in box body, described label is selected from radioactive substance, chemiluminescence or substance that show color, biology
Element, metal king crab, fluorescein, enzyme and nano material, described reagent includes：Hybridization solution, synergist, developer, Sample dilution, chain
Enzyme Avidin-HRP, chemical luminescence for liquid, liquid, concentrated cleaning solution, described Sample dilution is 0.05mol/L phosphate buffer,
PH=7.0, phosphate buffer is every liter and contains 16gNaCl, 0.8g KCl, 0.8g KH2PO4, 6.4g Na2HPO4Water-soluble
Liquid, described chemical luminescence for liquid A liquid for luminol content be 0.01M, p-cresol content be 0.001M pH=8.8 three hydroxyl first
Base aminomethane solution, described concentrated cleaning solution is the pH=7.4 containing volume fraction 0.05% tween 20,0.1mol/L phosphorus
Phthalate buffer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610798643.4A CN106405105A (en) | 2016-08-31 | 2016-08-31 | Chemiluminescence enzyme-linked immunoassay kit of human epididymis protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610798643.4A CN106405105A (en) | 2016-08-31 | 2016-08-31 | Chemiluminescence enzyme-linked immunoassay kit of human epididymis protein |
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| Publication Number | Publication Date |
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| CN106405105A true CN106405105A (en) | 2017-02-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610798643.4A Pending CN106405105A (en) | 2016-08-31 | 2016-08-31 | Chemiluminescence enzyme-linked immunoassay kit of human epididymis protein |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102890083A (en) * | 2012-09-28 | 2013-01-23 | 辽宁科骏生物有限公司 | Chemiluminescence substrate solution, kit containing same, and detection method applying chemiluminescence substrate solution or kit |
| CN102965449A (en) * | 2012-12-12 | 2013-03-13 | 芮屈生物技术（上海）有限公司 | In-situ hybridization detection kit of WFDC-2 gene as well as detection method and application thereof |
| US20130267439A1 (en) * | 2010-07-30 | 2013-10-10 | Vermillion, Inc. | Predictive markers and biomarker panels for ovarian cancer |
| CN103575890A (en) * | 2012-08-03 | 2014-02-12 | 北京勤邦生物技术有限公司 | Chemiluminescence assay kit of ractopamine (RAC) and application thereof |
| WO2014182896A1 (en) * | 2013-05-10 | 2014-11-13 | Johns Hopkins University | Compositions for ovarian cancer assessment having improved specificity |
| CN104730249A (en) * | 2013-12-20 | 2015-06-24 | 北京义翘神州生物技术有限公司 | Enzyme linked immunosorbent assay kit for detecting human epididymis secretory protein 4 |
| CN105277711A (en) * | 2014-07-21 | 2016-01-27 | 广州瑞博奥生物科技有限公司 | Enzyme-linked immunosorbent assay kit for detecting HE4 |
-
2016
- 2016-08-31 CN CN201610798643.4A patent/CN106405105A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130267439A1 (en) * | 2010-07-30 | 2013-10-10 | Vermillion, Inc. | Predictive markers and biomarker panels for ovarian cancer |
| CN103575890A (en) * | 2012-08-03 | 2014-02-12 | 北京勤邦生物技术有限公司 | Chemiluminescence assay kit of ractopamine (RAC) and application thereof |
| CN102890083A (en) * | 2012-09-28 | 2013-01-23 | 辽宁科骏生物有限公司 | Chemiluminescence substrate solution, kit containing same, and detection method applying chemiluminescence substrate solution or kit |
| CN102965449A (en) * | 2012-12-12 | 2013-03-13 | 芮屈生物技术（上海）有限公司 | In-situ hybridization detection kit of WFDC-2 gene as well as detection method and application thereof |
| WO2014182896A1 (en) * | 2013-05-10 | 2014-11-13 | Johns Hopkins University | Compositions for ovarian cancer assessment having improved specificity |
| CN104730249A (en) * | 2013-12-20 | 2015-06-24 | 北京义翘神州生物技术有限公司 | Enzyme linked immunosorbent assay kit for detecting human epididymis secretory protein 4 |
| CN105277711A (en) * | 2014-07-21 | 2016-01-27 | 广州瑞博奥生物科技有限公司 | Enzyme-linked immunosorbent assay kit for detecting HE4 |
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Application publication date: 20170215 |
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