CN106404881A - Method for detecting heavy metal elements entering into human body - Google Patents
Method for detecting heavy metal elements entering into human body Download PDFInfo
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- CN106404881A CN106404881A CN201610794904.5A CN201610794904A CN106404881A CN 106404881 A CN106404881 A CN 106404881A CN 201610794904 A CN201610794904 A CN 201610794904A CN 106404881 A CN106404881 A CN 106404881A
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- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 102
- 238000000034 method Methods 0.000 title claims abstract description 44
- 238000001514 detection method Methods 0.000 claims abstract description 47
- 210000004209 hair Anatomy 0.000 claims abstract description 41
- 238000000095 laser ablation inductively coupled plasma mass spectrometry Methods 0.000 claims abstract description 20
- 238000000608 laser ablation Methods 0.000 claims abstract description 16
- 231100000640 hair analysis Toxicity 0.000 claims abstract description 12
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 32
- 238000002386 leaching Methods 0.000 claims description 24
- 229910052785 arsenic Inorganic materials 0.000 claims description 19
- 239000003550 marker Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 230000003779 hair growth Effects 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 5
- 230000036541 health Effects 0.000 claims description 5
- 210000003128 head Anatomy 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 230000004044 response Effects 0.000 claims description 4
- 210000004761 scalp Anatomy 0.000 claims description 4
- 238000004088 simulation Methods 0.000 claims description 4
- 210000004243 sweat Anatomy 0.000 claims description 4
- 229910052793 cadmium Inorganic materials 0.000 claims description 3
- 229910052804 chromium Inorganic materials 0.000 claims description 3
- 229910052802 copper Inorganic materials 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 3
- 229910052745 lead Inorganic materials 0.000 claims description 2
- 238000001095 inductively coupled plasma mass spectrometry Methods 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000009545 invasion Effects 0.000 abstract 2
- 239000011148 porous material Substances 0.000 abstract 2
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 16
- 208000032839 leukemia Diseases 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 206010027439 Metal poisoning Diseases 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 208000019838 Blood disease Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000008763 Mercury poisoning Diseases 0.000 description 1
- 102000003505 Myosin Human genes 0.000 description 1
- 108060008487 Myosin Proteins 0.000 description 1
- 208000005374 Poisoning Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- GOLCXWYRSKYTSP-UHFFFAOYSA-N arsenic trioxide Inorganic materials O1[As]2O[As]1O2 GOLCXWYRSKYTSP-UHFFFAOYSA-N 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 208000010501 heavy metal poisoning Diseases 0.000 description 1
- 208000014951 hematologic disease Diseases 0.000 description 1
- 208000018706 hematopoietic system disease Diseases 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000009616 inductively coupled plasma Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 208000008127 lead poisoning Diseases 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000004118 muscle contraction Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention provides a method for detecting heavy metal elements entering into a human body. The method is used for detecting the contents of the heavy metal elements and invasion time of the heavy metal elements in the human body. The method comprises the following steps: a heavy metal element content linear equation Y=F(X) is firstly determined, the growth speed of hair of the human body that is invaded by the heavy metal elements to be detected=a centimetre/day is detected, LA-ICP-MS is used for detection, CPS (heavy metal elements to be detected) is detected, and the contents of the heavy metal elements to be detected are obtained according to Y=F(X); a distance b centimetre between a laser ablation pore and a root of hair on a hair sample is also detected, and the invasion time (day) of the heavy metal elements to be detected at the position of the laser ablation pore = b (centimetre)/a(centimetre/day) is calculated. The method has the advantages of simple operation, easy implement, reduced detection time, improved detection efficiency, lower detection limit, wider linearity range, and good repeatability; accordingly, the method for detecting heavy metal elements entering into the human body has good application prospects.
Description
Technical field
The invention belongs in vitro sample elements detection field, it is related to the side that a kind of detection enters the heavy metal element of human body
Method, the content and this heavy metal element for measuring this heavy metal element enters the time of human body.
Background technology
Heavy metal poisoning refers to the poisoning that heavy metal element or its compound cause, such as mercury poisoning, lead poisoning etc..Because weight
Metal can make the structure of protein that irreversible change occurs, thus affecting histocyte function, and then affects health,
For example internal enzyme cannot catalytic chemistry react, and the carrier of surface of cell membrane cannot be transported into nutriment, discharge metabolism
Waste, myosin and actin just cannot complete contraction of muscle, so internal cell just cannot obtain nutrition, exclusion is useless
Thing it is impossible to produce energy, lose by eucaryotic cell structure collapse and function.
In clinical medicine with medicolegal practice, exist and the in vitro sample from patient or victim is carried out with heavy metal unit
The demand of element detection.At present, the main method being related to the detection of contents of heavy metal elements in domestic and foreign literature includes：Atom is glimmering
Light spectroscopic methodology, atomic absorption spectrography (AAS), inductively coupled plasma method, AAS, liquid chromatography, inductive etc. from
Daughter mass spectrography (ICP-MS method) etc..Wherein, ICP-MS method, compared with other methods, has the advantage that：Once can survey simultaneously
Determine multiple element, save detection time, improve detection efficiency；Detection limit is relatively low, and the range of linearity is wider, reproducible.Therefore,
ICP-MS method is a kind of detection method being worth and making full use of and promote.
In clinical medicine with medicolegal practice, also there is the demand deducing the time that heavy metal element invades human body,
Judgement for the state of an illness or the evidence obtaining of case.However, there is no one kind in prior art can detect by heavy metal unit
This contents of heavy metal elements in the human body that element invades, can calculate the method that this heavy metal element enters the time of human body again.
Content of the invention
It is contemplated that overcoming defect present in prior art, and a kind of efficiently detection is provided to enter a huge sum of money for human body
The method belonging to element, the method can detect this contents of heavy metal elements in the human body being invaded by heavy metal element, and energy
Calculate the time that this heavy metal element enters human body.
Therefore, a first aspect of the present invention, there is provided a kind of method that detection enters the heavy metal element of human body, for surveying
The content of this heavy metal element fixed and the time of this heavy metal element entrance human body, the method comprises the steps：
(1) hair of clip health volunteer, is carried out using acetone, water, acetone successively, then, dries in 30 DEG C；
(2) by step (1) drying after hair shred, packet, and be separately immersed in variable concentrations containing this heavy metal
In the solution of element, leaching system 24 hours, obtain broken of the system of soaking；
(3) take out described leaching system broken, is carried out using acetone, water, acetone successively, then, dries in 30 DEG C, system
Obtain broken of dried leaching system；Quantitative determination is carried out to broken of described dried leaching system using ICP-MS, records described
The content of the heavy metal element in broken of dried leaching system, as the Y of contents of heavy metal elements linear equation；
(4) adopt in hair34S, as internal standard, records dried leaching system described in step (3) using LA-ICP-MS
Broken in this heavy metal element response CPS(this heavy metal element)With34Response CPS of S( 34 S), then obtain heavy metal element
The X=CPS of content linear equation(this heavy metal element)/CPS( 34 S)；
(5) repeatedly measured according to above-mentioned steps (1)～(4), determined this contents of heavy metal elements linear equation Y=F
(X)；
(6) the head rear pillow part a handful of hairs of selection in the human body being invaded by heavy metal element to be measured carry out dye marker,
The length of the hair newly growing is measured, thus recording the natural on-off cycles of hair growth of the human body being invaded by heavy metal element to be measured behind some skies
Speed=a centimetre/day；Meanwhile, choose several undyed hairs near the above-mentioned hair carrying out dye marker, paste scalp
Cut, carry out LA-ICP-MS detection, thus record the hair sample of the human body being invaded by heavy metal element to be measured
CPS(heavy metal element to be measured), and according to Y=F (X), you can obtain the content of this heavy metal element to be measured；
(7) in the LA-ICP-MS detection process in step (6), the laser ablation pitch-row also recording on hair sample is sent out
The distance of root is b centimetre, thus calculate this at this laser ablation hole heavy metal element to be measured enter human body time (my god)
=b (centimetre)/a (centimetre/day).
Preferably, enter in above-mentioned detection in the method for heavy metal element of human body, described heavy metal element includes：Pb,
Cd, Cr, Cu, As, Hg, Mn.
Preferably, enter in above-mentioned detection in the step (2) of method of the heavy metal element of human body：Step (1) is dried
Hair scissors afterwards is broken to 0.1～0.3 millimeter.
Preferably, enter in the method for heavy metal element of human body in above-mentioned detection, described LA-ICP-MS detection is using even
Continuous simple scan operation obtains the laser ablation hole that row are spaced.
Preferably, enter in above-mentioned detection in the step (2) of method of the heavy metal element of human body：Described variable concentrations
Solution containing this heavy metal element adopts manual simulation's sweat as solvent.
Preferably, enter in the method for heavy metal element of human body in above-mentioned detection, described by heavy metal element to be measured
In the case that the human body invading is corpse, natural on-off cycles of hair growth speed=1 centimetre of described corpse/month.
Compared with prior art, technical scheme provided by the present invention has advantages below：
Described detection enters the method for the heavy metal element of human body in detecting the human body being invaded by heavy metal element
Moreover it is possible to the time that this heavy metal element enters human body is gone out with less error calculation while this contents of heavy metal elements；Described
Detection enter human body heavy metal element method simple to operate it is easy to implement；Described detection enters the heavy metal element of human body
Method reasonably make use of LA-ICP-MS detection method, once can measure multiple element simultaneously, save detection time, carry
High detection efficiency, and detection limit is relatively low, the range of linearity is wider, reproducible；Therefore, described detection enters the heavy metal unit of human body
The method of element can get extensive application, has a good application prospect.
Brief description
Fig. 1 is the schematic diagram of contents of heavy metal elements linear equation Y=F (X) of the present invention；
Fig. 2 be for LA-ICP-MS detection degrade after hair sample micro-image, show multiple laser ablation
Hole；
Fig. 3 is arsenic in the leukemia patient hair after the arsenic agent treatment of five courses for the treatment of described in the embodiment of the present invention 1
The concentration profile of element.
Specific embodiment
A first aspect of the present invention, there is provided a kind of method that detection enters the heavy metal element of human body, for measuring this
The content of heavy metal element and the time of this heavy metal element entrance human body, the method comprises the steps：(1) clip health is subject to
The hair of examination person, is carried out using acetone, water, acetone successively, then, dries in 30 DEG C；
(2) by step (1) drying after hair shred, packet, and be separately immersed in variable concentrations containing this heavy metal
In the solution of element, leaching system 24 hours, obtain broken of the system of soaking；
(3) take out described leaching system broken, is carried out using acetone, water, acetone successively, then, dries in 30 DEG C, system
Obtain broken of dried leaching system；Quantitative determination is carried out to broken of described dried leaching system using ICP-MS, records described
The content of the heavy metal element in broken of dried leaching system, as the Y of contents of heavy metal elements linear equation；
(4) adopt in hair34S, as internal standard, records dried leaching system described in step (3) using LA-ICP-MS
Broken in this heavy metal element response CPS(this heavy metal element)With34Response CPS of S( 34 S), then obtain heavy metal element
The X=CPS of content linear equation(this heavy metal element)/CPS( 34 S)；
(5) repeatedly measured according to above-mentioned steps (1)～(4), determined this contents of heavy metal elements linear equation Y=F
(X)；Specifically, record X1, X2, X3, X4, X5 ... and correspondingly record Y1Y2Y3Y4Y5 ..., thus being entered by software etc.
Row linear regression analysis obtains linear equation Y=F (X), as shown in Figure 1；
(6) the head rear pillow part a handful of hairs of selection in the human body being invaded by heavy metal element to be measured carry out dye marker,
The length of the hair newly growing is measured, thus recording the natural on-off cycles of hair growth of the human body being invaded by heavy metal element to be measured behind some skies
Speed=a centimetre/day；Meanwhile, choose several undyed hairs near the above-mentioned hair carrying out dye marker, paste scalp
Cut, carry out LA-ICP-MS detection, thus record the hair sample of the human body being invaded by heavy metal element to be measured
CPS(heavy metal element to be measured), and according to Y=F (X), you can obtain the content of this heavy metal element to be measured；
(7) in the LA-ICP-MS detection process in step (6), the laser ablation pitch-row also recording on hair sample is sent out
The distance of root is b centimetre, thus calculate this at this laser ablation hole heavy metal element to be measured enter human body time (my god)
=b (centimetre)/a (centimetre/day).As shown in Fig. 2 high-visible multiple laser ablation holes on this hair sample.
In a preferred embodiment, described heavy metal element includes：Pb, Cd, Cr, Cu, As, Hg, Mn.
In a preferred embodiment, described detection enters in the step (2) of the method for heavy metal element of human body：To walk
Suddenly the hair scissors after (1) dries is broken to 0.1～0.3 millimeter.
In a preferred embodiment, enter in the method for heavy metal element of human body in above-mentioned detection, described LA-ICP-
MS detection obtains, using the operation of continuous simple scan, the laser ablation hole that row are spaced, as shown in Figure 2.
In a preferred embodiment, described detection enters in the step (2) of the method for heavy metal element of human body：Described
The solution containing this heavy metal element of variable concentrations adopts manual simulation's sweat as solvent.
In a preferred embodiment, in the case that the described human body being invaded by heavy metal element to be measured is corpse, institute
State natural on-off cycles of hair growth speed=1 centimetre of corpse/month.
With reference to specific embodiment, the present invention is further elaborated, but the present invention is not limited to following examples.
Each step in methods described is routine operation if no special instructions；Described equipment if no special instructions all can be from open business
Approach obtains.
Embodiment 1
One leukemia patient have passed through the arsenic agent treatment of five courses for the treatment of, now measures this according to the method described in the present invention white
Blood disease patient's hair in arsenic content (concentration) and calculate the time that arsenic element enters in this leukemia patient body, specifically such as
Under：
(1) hair of clip health volunteer, is carried out using acetone, water, acetone successively, then, dries in 30 DEG C；
(2) hair scissors after step (1) drying is broken to 0.2 millimeter, packet, and it is separately immersed in containing of variable concentrations
As2O3Manual simulation's sweat in, leaching system 24 hours, obtain soak system broken；
(3) take out described leaching system broken, is carried out using acetone, water, acetone successively, then, dries in 30 DEG C, system
Obtain broken of dried leaching system；Quantitative determination is carried out to broken of described dried leaching system using ICP-MS, records described
The content of the arsenic element in broken of dried leaching system, as the Y of arsenogen cellulose content linear equation；
(4) adopt in hair34S, as internal standard, records dried leaching system described in step (3) using LA-ICP-MS
Broken response CPS sending out arsenic element middle(As)With34Response CPS of S( 34 S), then obtain the X=of arsenogen cellulose content linear equation
CPS(As)/CPS( 34 S)；Wherein, instrument has：Icp mses (ICP-MS), Agilent company of the U.S.,
Model：7500ce；Laser ablation sampling system (LA), New wave company of the U.S., model：UP213；Wherein, LA-ICP-MS inspection
Survey main working parameters as shown in table 1 below：
Table 1：LA-ICP-MS detects main working parameters
(5) repeatedly measured according to above-mentioned steps (1)～(4), determined arsenogen cellulose content linear equation Y=F (X)；
(6) use arsenic agent treatment in this leukemia patient first first day, chooses from the head rear pillow part of this leukemia patient
A handful of hairs carry out dye marker, and the length measuring the hair newly growing after 159 days is 6.5 centimetres, thus calculating this
Leukemia patient natural on-off cycles of hair growth speed=0.04 centimetre/day；Meanwhile, the arsenical of five courses for the treatment of carrying out in this leukemia patient is controlled
During treatment, near the above-mentioned hair carrying out dye marker, repeatedly choose several undyed hairs, patch scalp is cut, and carries out
LA-ICP-MS detects, thus recording the CPS of the hair sample of this patient(As to be measured), and according to Y=F (X), obtain this arsenogen to be measured
The content of element；As shown in figure 3, can clearly be observed that in this leukemia patient hair after the arsenic agent treatment of five courses for the treatment of
The concentration profile of arsenic element, i.e. the content of the arsenic element of different times.And, it can be seen that the concentration of arsenic element exists
Accumulation and the fluctuation of metabolism, with each course for the treatment of as a cycle.
(7) in the LA-ICP-MS detection process in step (6), also carried out following measurement：In shown 5 courses for the treatment of
In arsenic agent treatment, when each course for the treatment of starts medication collection hair swatch, the laser ablation pitch-row of the hair the top newly growing
The distance of root of hair respectively is 6.5 centimetres, 4.8 centimetres, 3.5 centimetres, 2.1 centimetres, 0.9 centimetre；Thus can calculate respectively
This arsenic element at this laser ablation hole enters the time of human body, refers to table 2 below：
Table 2 arsenic element enters the contrast of human body real time and the time of calculating
In upper table 2, α：Every course for the treatment of starts medication the laser of the stylish hair the top growing of hair sample collection
Degrade the distance of pitch-row root of hair；β:Every course for the treatment of starts medication time during hair sample collection；γ:It is calculated by formula
Arsenic element enter the human body time.
Above the specific embodiment of the present invention is described in detail, but it has been intended only as example, the present invention has not limited
It is formed on particular embodiments described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and
Substitute also all among scope of the invention.Therefore, the impartial conversion made without departing from the spirit and scope of the invention and
Modification, all should cover within the scope of the invention.
Claims (4)
1. a kind of method that detection enters the heavy metal element of human body, for measuring content and this heavy metal of this heavy metal element
Element enters the time of human body it is characterised in that comprising the steps：
（1）The hair of clip health volunteer, is carried out using acetone, water, acetone successively, then, dries in 30 DEG C；
（2）By step（1）Hair after drying shreds, packet, and be separately immersed in variable concentrations containing this heavy metal element
Solution in, leaching system 24 hours, obtain soak system broken；
（3）Take out described leaching system broken, is carried out using acetone, water, acetone successively, then, dries in 30 DEG C, is obtained dry
Broken of leaching system after dry；Quantitative determination is carried out to broken of described dried leaching system using ICP-MS, records described drying
The content of the heavy metal element in broken of leaching system afterwards, as the Y of contents of heavy metal elements linear equation；
（4）Using in hair34S, as internal standard, records step using LA-ICP-MS（3）Described in dried leaching system broken
Response CPS of this heavy metal element in sending out（This heavy metal element）With34Response CPS of S（ 34 S）, then obtain contents of heavy metal elements
The X=CPS of linear equation（This heavy metal element）/ CPS（ 34 S）；
（5）According to above-mentioned steps（1）~（4）Repeatedly measured, determined this contents of heavy metal elements linear equation Y=F (X)；
（6）Choose a handful of hairs in the head rear pillow part of the human body being invaded by heavy metal element to be measured and carry out dye marker, some
The length of the hair newly growing is measured, thus recording the natural on-off cycles of hair growth speed of the human body being invaded by heavy metal element to be measured after it
=a centimetre/day；Meanwhile, several undyed hairs are chosen near the above-mentioned hair carrying out dye marker, patch scalp is cut,
Carry out LA-ICP-MS detection, thus recording the CPS of the hair sample of the human body being invaded by heavy metal element to be measured（Heavy metal element to be measured）,
And according to Y=F (X), you can obtain the content of this heavy metal element to be measured；
（7）In step（6）In LA-ICP-MS detection process in, also record laser ablation pitch-row root of hair on hair sample
Distance is b centimetre, thus this heavy metal element to be measured calculating at this laser ablation hole enters the time of human body（My god）= b
（Centimetre）/ a（Centimetre/day）.
2. detection according to claim 1 enters the method for the heavy metal element of human body it is characterised in that described heavy metal
Element includes：Pb, Cd, Cr, Cu, As, Hg, Mn.
3. detection according to claim 1 enters the method for the heavy metal element of human body it is characterised in that described LA-
ICP-MS detection obtains, using the operation of continuous simple scan, the laser ablation hole that row are spaced.
4. detection according to claim 1 enters the method for the heavy metal element of human body it is characterised in that step（2）In：
The solution containing this heavy metal element of described variable concentrations adopts manual simulation's sweat as solvent.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105074441A (en) * | 2013-02-28 | 2015-11-18 | 一般社团法人矿物研究会 | Method for exanimation of element in living body |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105074441A (en) * | 2013-02-28 | 2015-11-18 | 一般社团法人矿物研究会 | Method for exanimation of element in living body |
Non-Patent Citations (4)
| Title |
|---|
| MINJUAN HUANG等: "Risk assessment of arsenic and other metals via atmospheric particls，and effects of atmospheric exposure and other demographic factors on their accumulations in human scalp hair in urban area of Guangzhou，China", 《ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY》 * |
| 李健等: "人发中重金属含量的测定分析", 《农业与技术》 * |
| 莫少锋等: "人体重金属检测方法", 《华夏医学》 * |
| 马栋等: "激光剥蚀电感耦合等离子体质谱技术检测车辆油漆金属元素", 《中国司法鉴定》 * |
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