CN106397674A - Temperature-sensitive postoperative adhesion cellulose modifying material and preparation method and application thereof - Google Patents
Temperature-sensitive postoperative adhesion cellulose modifying material and preparation method and application thereof Download PDFInfo
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- CN106397674A CN106397674A CN201610846952.4A CN201610846952A CN106397674A CN 106397674 A CN106397674 A CN 106397674A CN 201610846952 A CN201610846952 A CN 201610846952A CN 106397674 A CN106397674 A CN 106397674A
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
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- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
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- A—HUMAN NECESSITIES
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- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
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- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/009—Materials resorbable by the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/042—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L31/145—Hydrogels or hydrocolloids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L31/148—Materials at least partially resorbable by the body
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Abstract
The invention discloses a temperature-sensitive postoperative adhesion cellulose modifying material and a preparation method and application thereof. Sulfobetaines type zwitterionic monomers with anti-cell-adhesion or protein non-specificity adsorption property are grafted and co-polymerized onto a temperature-sensitive methylcellulose main chain, and a grafted copolymer aqueous solution is prepared by a low-temperature dissolution method. At the room temperature, the grafted copolymer aqueous solution is viscous liquid which is easy to inject or coat, can become hydrogel spontaneously and rapidly at the temperature of the human body, and has reversible sol-gel phase transformation characteristic. The temperature-sensitive postoperative adhesion cellulose modifying material is good in biological compatibility, and high in cell adhesion resistance and tissue adhesion resistance, is suitable for adhesion prevention of complicated surgical wounds or wound parts, and has high operation convenience and postoperative adhesion prevention effectiveness.
Description
Technical field
The invention belongs to bio-medical hydrogel material preparing technical field is and in particular to a kind of Thermo-sensitive Prevention of postsurgical adhesion
Cellulose modified material and preparation method and application.
Background technology
With a large amount of developments of every surgical operation, postoperative biological tissue adhesion problems become increasingly conspicuous.Adhesion is usually hand
Improper connection is there occurs between wound location that postoperative wound, infection or other factors are led to and surrounding tissue or organ
And the anomaly sxtructure being formed.Post-operation adhesion not only affects surgical effect, can also result in serious postoperative complications, such as small intestine stalk
Resistance, women's pelvic adhesion etc., make operative failure.Therefore, find effective method prevention of postoperative adhesion biomedical engineering always
The important topic in field.
So far the most widely used means of preventing adhesion are implantation physics barrier materials, by separating wounded tissue and surrounding
Organ is thus prevent adhesion.Conventional adhesion-reducing barrier mainly has following three classes：One is solid state film material, such as patent
Disclosed in a kind of post-operation adhesion preventing membrane material, patent CN104436317A disclosed in CN103044700A, a kind of post-operation adhesion preventing is high
Molecular film etc., such material is not because of advance comprising, easy to operate and be difficult to be applied to the wounds of some complexity in surgical procedure
Position, its application is restricted；Two classes are water liquid class materials, and as disclosed in patent CN103271939A, a kind of operation is with antiseized
Even flushing liquor, this kind of material has good mobility, injectable or the wound location being coated on complexity, but easy loss, resistance
Fall short of every the time, effect limited；Three is Hydrogels material, has mobility under certain condition, also injectable or coating
In complicated surgical wound surface or wound location, and the obstruct time is relatively long, and what such as the scholar such as Yeo reported can in-situ cross-linked pair of group
Part hydrogel (Biomaterials, 2006,27:4698-4705；Biomaterials,2007,28:3418-3426), but its
The chemical cross-linking agent of certain toxicity is employed during preparation.Consider from the mechanism of action preventing postoperative tissue adhesion, by relevant physics
Obstruct means are successfully applied to Prevention of postsurgical adhesion, and adhesion-reducing barrier should have good anti-protein non-specific adsorption
Or cell adherence performance, but above-mentioned physical barrier material does not generally have this respect performance, causes its application effect not ideal enough.
Content of the invention
In order to overcome the shortcoming of prior art with not enough, the primary and foremost purpose of the present invention is to provide a kind of Thermo-sensitive to prevent postoperative
The preparation method of the cellulose modified material of adhesion, that is, with there is temperature sensitive and excellent biocompatibility methylcellulose as main chain,
With there is Anti cell adhesion or protein non-specific adsorption property sulphonic acid betaine type amphoteric ion polymer for side chain structure
Build the cellulose modified material of Prevention of postsurgical adhesion.
Another object of the present invention is to providing the Thermo-sensitive Prevention of postsurgical adhesion cellulose obtaining based on above-mentioned preparation method
Material modified.This cellulose modified material does not use the chemical cross-linking agent of genotoxic potential, and its aqueous solution is to be easy at room temperature
Injection or coating viscous liquid, under human body temperature then can spontaneous quick formation hydrogel, there is reversible sol-gel phase
Conversion characteristic.
It is still another object of the present invention to provide the cellulose modified material of above-mentioned Thermo-sensitive Prevention of postsurgical adhesion is as anti-caecum
The purposes of post-operation adhesion material.
The purpose of the present invention is achieved through the following technical solutions：
A kind of preparation method of the cellulose modified material of Thermo-sensitive Prevention of postsurgical adhesion, specifically includes following steps：
(1) in methylated cellulose aqueous solution, zwitterionic monomer methacryloylethyl sulfobetaines, initiation are added
Agent and N, N, N', N'- tetramethylethylenediamine, carry out free yl graft polymerization reaction under nitrogen or argon, reaction completes
Dialysis freeze-drying afterwards, obtains methylcellulose grafting methacryloylethyl sulfobetaines zwitterionic monomer copolymerization
Thing；
(2) the methylcellulose grafting methacryloylethyl sulfobetaines zwitterionic monomer that step (1) is obtained
Copolymer is dissolved in buffer solution, is configured to aqueous copolymers solution, the cellulose modified material of as described Thermo-sensitive Prevention of postsurgical adhesion.
Methylated cellulose aqueous solution described in step (1), is that methylcellulose is added to 70～80 DEG C of hot distilled waters
In, then it is extremely fully transparent and prepared to be placed in continuation stirring in ice-water bath.Described methylated cellulose aqueous solution mass fraction is preferably
1.0%～4.0%.
Methacryloylethyl sulfobetaines described in step (1) and the Methyl cellulose in methylated cellulose aqueous solution
The mass ratio of element is (1:2)～(1:8).
Initiator described in step (1), N, N, N', N'- tetramethylethylenediamine and methacryloylethyl sulfobetaines
The mass ratio of alkali is (1:1:20)～(1:1:10), more preferably 1:1:10.
The condition of the free yl graft polymerization reaction described in step (1) is preferably 25～45 DEG C, time 12～24h.
Initiator described in step (1) is at least one in ammonium persulfate, potassium sulfate and sodium peroxydisulfate, preferably
Ammonium sulfate.
Buffer solution described in step (2) is disodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution (pH=7.4), acetic acid-acetic acid
Sodium buffer solution (pH=6.8), citric acid-sodium citrate buffer solution (pH=7.2) or citric acid-sodium hydroxide-hydrochloride buffer
(pH=7.0).
Aqueous copolymers solution described in step (2), is that methylcellulose is grafted methacryloylethyl sulfobetaines
Alkali zwitterionic monomer copolymer adds in buffer solution, then is placed in ice-water bath and stirs to being completely dissolved, and puts in 4 DEG C of refrigerators
Put overnight and be obtained.The mass fraction of described aqueous copolymers solution is preferably 10%～20%.
Present invention also offers a kind of Thermo-sensitive Prevention of postsurgical adhesion cellulose being prepared by above-mentioned preparation method is changed
Property material.
Present invention also offers the cellulose modified material of above-mentioned Thermo-sensitive Prevention of postsurgical adhesion prevent adhesion as internal wound deposited
The application process of material, for example can be used as anti-caecum post-operation adhesion material.The described cellulose modified material of Thermo-sensitive Prevention of postsurgical adhesion
Material can be used alone, and uses after also can adding other external used medicines.
The described cellulose modified material of Thermo-sensitive Prevention of postsurgical adhesion is used alone, and refers to directly be grafted methylcellulose
Methacryloylethyl sulfobetaines zwitterionic monomer aqueous copolymers solution is injected in internal wound, under body temperature
Form hydrogel.
The described cellulose modified material of Thermo-sensitive Prevention of postsurgical adhesion uses after adding other external used medicines, and its step is such as
Under：
(A) weigh 1-100 milligram medicine (as ampicillin), add methylcellulose grafting methacryloylethyl
In sulfobetaines zwitterionic monomer aqueous copolymers solution, it is placed in after low temperature stirring and dissolving in 4 DEG C of refrigerators and stands overnight, be obtained
The aqueous copolymers solution of embedding medicinal, is the viscous liquid being easy to inject under room temperature；
(B) aqueous copolymers solution of step (A) gained embedding medicinal is injected in internal wound, gets final product shape under body temperature
Become the hydrogel of carrying medicament.
Compared with prior art, the present invention has advantages below and beneficial effect：
(1) primary raw material that the present invention adopts is abundance and the methyl fibre with biological safety and biological degradability
Dimension element, low price.
(2) present invention does not use chemical cross-linking agent, only leans on the temperature sensitive peculiar property of methylcellulose segment in aqueous
It is physical crosslinking, safety non-toxic.
(3) gelling temperature of the cellulose modified material of Thermo-sensitive Prevention of postsurgical adhesion of gained of the present invention is 30～35 DEG C,
Under room temperature be easy to inject or coat viscous liquid, under human body temperature then can spontaneous quick formation hydrogel, have reversible
Sol-gel phase in version characteristic.
(4) the cellulose modified material of Thermo-sensitive Prevention of postsurgical adhesion of gained of the present invention can be used as internal wound prevent adhesion deposited
Material, has certain application prospect in biomedical materials field.
(5) present invention operation is simple, and low for equipment requirements, applicability is wide.
Brief description
Fig. 1 is the process chart preparing the cellulose modified material of Thermo-sensitive Prevention of postsurgical adhesion.
Fig. 2 is the dynamic temperature of the Thermo-sensitive Prevention of postsurgical adhesion cellulose modified material hydrogel sample that embodiment 2 is obtained
Scanning curve (G'：Storage modulus, G "：Loss modulus)：△ represents methylcellulose grafting methacryloylethyl sulfobetaines
The G' temperature curve of alkali zwitterionic monomer copolymer aquagel；Represents methylcellulose grafting methacryloylethyl sulphur
The G of base beet alkali ampholytic ion monomer copolymer aquagel " temperature curve.
Fig. 3 is the cell adherence growing state microphotograph of the cellulose modified material of Thermo-sensitive Prevention of postsurgical adhesion.
Fig. 4 is the cellulose modified materials application of Thermo-sensitive Prevention of postsurgical adhesion in the photo of rat cecal wound model.
Specific embodiment
With reference to embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention do not limit
In this.Unless stated otherwise, involved reagent in embodiment, method are reagent commonly used in the art and method.
Embodiment 1
(1) weigh the distilled water that the methylcellulose (MC) that 1.0 grams of viscosity are 15cP is added to 80 DEG C of 50mL, then will
Flask is placed in continuation stirring in ice-water bath makes it be completely dissolved, transparent liquid.Weigh 0.5 gram of methacryloylethyl sulphur again
Base glycine betaine (DMAPS) is added to flask and continues to stir, and is passed through nitrogen protection.It is subsequently adding 50 milligrams of ammonium persulfates
(APS) initiator and 65 microlitres of N are made, N, N', N'- tetramethylethylenediamine (TEMED, density 0.775g/mL, similarly hereinafter) make to promote
Agent, reacts 24 hours under the conditions of 25 DEG C in a nitrogen atmosphere.After having reacted, reactant liquor is transferred completely into molecular cut off is
In 8000 bag filter, deionized water is dialysed 3 days, obtains methylcellulose grafting methacryloylethyl sulfo group sweet after being lyophilized
Dish alkali zwitterionic monomer copolymer.
(2) weigh the methylcellulose grafting methacryloylethyl sulfobetaines amphion that 1 gram of step (1) is obtained
Monomer copolymer, and add the disodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution of 5 milliliters of pH=7.4, it is placed in ice-water bath and constantly stir
Mix and so that it is dissolved.4 DEG C of refrigerators stand overnight after so that bubble is escaped, obtain the aqueous copolymers solution that mass fraction is 20%,
It is placed in preserving at 4 DEG C.
(3) aqueous copolymers solution is heated to 37 DEG C, that is, is changed into immobilising gel, obtain methylcellulose grafting
Methacryloylethyl sulfobetaines zwitterionic monomer copolymer aquagel sample.
Embodiment 2
(1) weigh the distilled water that the methylcellulose (MC) that 1.2 grams of viscosity are 15cP is added to 80 DEG C of 50mL, then will
Flask is placed in continuation stirring in ice-water bath makes it be completely dissolved, transparent liquid.Weigh 0.3 gram of methacryloylethyl sulphur again
Base glycine betaine (DMAPS) is added to flask and continues to stir, and is passed through nitrogen protection.It is subsequently adding 30 milligrams of ammonium persulfates
(APS) initiator and 40 microlitres of N are made, N, N', N'- tetramethylethylenediamine (TEMED) makees accelerator, 45 DEG C in a nitrogen atmosphere
Under the conditions of react 12 hours.After having reacted, reactant liquor is transferred completely in the bag filter that molecular cut off is 8000, spend from
Sub- water is dialysed 3 days, obtains methylcellulose grafting methacryloylethyl sulfobetaines zwitterionic monomer copolymerization after being lyophilized
Thing.
(2) weigh the methylcellulose grafting methacryloylethyl sulfobetaines amphion that 1 gram of step (1) is obtained
Monomer copolymer, and add the disodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution of 10 milliliters of pH=7.4, it is placed in ice-water bath constantly
Stirring makes it dissolve.4 DEG C of refrigerators stand overnight after so that bubble is escaped, obtain the copolymer that mass fraction is 10% water-soluble
Liquid, is placed in preserving at 4 DEG C.
(3) aqueous copolymers solution is heated to 37 DEG C, that is, is changed into immobilising gel, obtain methylcellulose grafting
Methacryloylethyl sulfobetaines zwitterionic monomer copolymer aquagel sample.
(4) gelling temperature of copolymer aquagel is surveyed using the senior rotational rheometer of ARES/RFS type (TA, USA)
Fixed, fixture is 25 millimeters of plate jig, at 20 DEG C by the aqueous copolymers solution uniform application of 1mL 10% to flat board on, stand
It is engraved in and measure under dynamic temperature sweep pattern.Measuring condition：Frequency (Frequency) is 1rad/s, deformation (Strain)
For 1%, scan temperature range and be 25～40 DEG C, heating rate is 1 DEG C/min, the spacing (Gap) of plate jig is actual by experiment
Adjustment is defined, and this experiment typically takes 0.8 millimeter.
Result as shown in Fig. 2 when storage modulus G' be more than loss modulus G " when, sample has formed gel state, now
Temperature is then the gelling temperature of sample, and its value is 33 DEG C.
Embodiment 3
(1) reference implementation example 2 makes the methylcellulose grafting methacryloylethyl sulfo group that mass fraction is 10%
Beet alkali ampholytic ion monomer aqueous copolymers solution, is placed in preserving at 4 DEG C.
(2) weigh the methylcellulose that 1 gram of viscosity is 15cP and add the disodium hydrogen phosphate-phosphoric acid of 10 milliliters of pH=7.4
Sodium dihydrogen buffer solution, being placed in ice-water bath to be stirred continuously makes it dissolve.4 DEG C of refrigerators stand overnight after so that bubble is escaped, obtain
The methylated cellulose aqueous solution being 10% to mass fraction, is placed in preserving at 4 DEG C.
(3) methylated cellulose aqueous solution and methylcellulose is used to be grafted methacryloylethyl sulfobetaines both sexes respectively
The half area of ion monomer aqueous copolymers solution uniform fold Tissue Culture Plate, the other half area of culture plate is used as blank
Comparison.Culture plate is placed in 37 DEG C of cell culture incubator, so that gel sample is sufficiently formed.Will be equal for people's nasopharyngeal epithelial cells NP69
Even kind, on culture plate, is placed in overnight incubation in 37 DEG C of cell culture incubators containing 5%CO2, then uses tetrazolium bromide (MTT) to thin
Born of the same parents are dyeed, and the adhesion growing state with microscope observation of cell.
(4) result is as shown in Figure 3 it is seen that there is more cell adherence to grow on methyl cellulose hydrogel sample surfaces,
And on methylcellulose grafting methacryloylethyl sulfobetaines zwitterionic monomer copolymer aquagel sample surfaces
Cell number is less, illustrates that copolymer aquagel has the property of Anti cell adhesion growth.
Embodiment 4
(1) reference implementation example 2 makes the methylcellulose grafting methacryloylethyl sulfo group that mass fraction is 10%
Beet alkali ampholytic ion monomer aqueous copolymers solution, is placed in preserving at 4 DEG C.
(2) weigh the methylcellulose that 1 gram of viscosity is 15cP and add the disodium hydrogen phosphate-phosphoric acid of 10 milliliters of pH=7.4
Sodium dihydrogen buffer solution, being placed in ice-water bath to be stirred continuously makes it dissolve.4 DEG C of refrigerators stand overnight after so that bubble is escaped, obtain
The methylated cellulose aqueous solution being 10% to mass fraction, is placed in preserving at 4 DEG C.
(3) weigh 100 milligrams of ampicillins, be added to the aqueous copolymers solution that step (1) is obtained, low temperature stirs to complete
CL, is obtained the aqueous copolymers solution of embedding ampicillin, is placed in preserving at 4 DEG C.
(4) weigh 100 milligrams of ampicillins, be added to the methylated cellulose aqueous solution that step (2) is obtained, low temperature stirs
To being completely dissolved, the methylated cellulose aqueous solution of embedding ampicillin is obtained, is placed in preserving at 4 DEG C.
(5) with 3% amobarbital (30 mg/kg), intraperitoneal injection of anesthesia is carried out to 200～250 grams of cleaning grade SD rats
Afterwards, cut off Mao Bingyong 75% alcohol disinfecting of belly.On super-clean bench, after SD rat laparotomy, caecum is scratched together about
0.5 centimetre of wound, then injection loads the methylated cellulose aqueous solution of ampicillin and loads ampicillin respectively
Methylcellulose grafting methacryloylethyl sulfobetaines zwitterionic monomer aqueous copolymers solution covers wound.Standing 5
Minute is formed after gel after it, caecum is resetted, abdominal incision operation suture thread is sewed on, and continues to feed.
(6) after performing the operation one week, SD rat is put to death, again cut abdominal incision open, observe the adhesion feelings of former caecum wound
Condition.Result is as shown in Figure 4 it is seen that the adhesion between caecum and caecum using the rat of methyl cellulose hydrogel, and uses
Methylcellulose is grafted the former caecum of the rat of methacryloylethyl sulfobetaines zwitterionic monomer copolymer aquagel
Wound healing is normal.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention are not subject to above-described embodiment
Limit, other any Spirit Essences without departing from the present invention and the change made under principle, modification, replacement, combine, simplify,
All should be equivalent substitute mode, be included within protection scope of the present invention.
Claims (10)
1. a kind of preparation method of the cellulose modified material of Thermo-sensitive Prevention of postsurgical adhesion is it is characterised in that comprise the steps：
(1) in methylated cellulose aqueous solution, add zwitterionic monomer methacryloylethyl sulfobetaines, initiator and
N, N, N', N'- tetramethylethylenediamine, carries out free yl graft polymerization reaction under nitrogen or argon, after the completion of reaction thoroughly
Analysis freeze-drying, obtain methylcellulose grafting methacryloylethyl sulfobetaines zwitterionic monomer copolymer；
(2) the methylcellulose grafting methacryloylethyl sulfobetaines zwitterionic monomer copolymerization that step (1) is obtained
Thing is dissolved in buffer solution, is configured to aqueous copolymers solution.
2. the preparation method of the cellulose modified material of a kind of Thermo-sensitive Prevention of postsurgical adhesion according to claim 1, its feature
It is, the methylcellulose in the methacryloylethyl sulfobetaines described in step (1) and methylated cellulose aqueous solution
Mass ratio be (1:2)～(1:8).
3. the preparation method of the cellulose modified material of a kind of Thermo-sensitive Prevention of postsurgical adhesion according to claim 1, its feature
It is, the initiator described in step (1), N, N, N', N'- tetramethylethylenediamine and methacryloylethyl sulfobetaines
Mass ratio is (1:1:20)～(1:1:10).
4. the preparation method of the cellulose modified material of a kind of Thermo-sensitive Prevention of postsurgical adhesion according to claim 1, its feature
It is, the condition of the free yl graft polymerization reaction described in step (1) is 25～45 DEG C, time 12～24h.
5. the preparation method of the cellulose modified material of a kind of Thermo-sensitive Prevention of postsurgical adhesion according to claim 1, its feature
It is, the methylated cellulose aqueous solution described in step (1), be that methylcellulose is added in 70～80 DEG C of hot distilled waters,
It is placed in continuation stirring in ice-water bath more extremely fully transparent and prepared.
6. the preparation method of the cellulose modified material of a kind of Thermo-sensitive Prevention of postsurgical adhesion according to claim 1, its feature
It is, the initiator described in step (1) is at least one in ammonium persulfate, potassium sulfate and sodium peroxydisulfate.
7. the preparation method of the cellulose modified material of a kind of Thermo-sensitive Prevention of postsurgical adhesion according to claim 1, its feature
It is, the buffer solution described in step (2) is disodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution, acetic acid-sodium acetate buffer solution, lemon
Lemon acid-sodium citrate buffer solution or citric acid-sodium hydroxide-hydrochloride buffer.
8. the preparation method of the cellulose modified material of a kind of Thermo-sensitive Prevention of postsurgical adhesion according to claim 1, its feature
It is, the aqueous copolymers solution described in step (2), be that methylcellulose is grafted methacryloylethyl sulfobetaines two
Property ion monomer copolymer add in buffer solution, then be placed in ice-water bath and stir to being completely dissolved, and placed in 4 DEG C of refrigerators
Night and be obtained.
9. a kind of cellulose modified material of Thermo-sensitive Prevention of postsurgical adhesion is it is characterised in that it is by any one of claim 1 to 8 institute
A kind of preparation method of the cellulose modified material of Thermo-sensitive Prevention of postsurgical adhesion stated prepares.
10. the cellulose modified material of Thermo-sensitive Prevention of postsurgical adhesion described in claim 9 prevents adhesion dressing as internal wound
Application.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610846952.4A CN106397674B (en) | 2016-09-23 | 2016-09-23 | A kind of cellulose modified material of Thermo-sensitive Prevention of postsurgical adhesion and the preparation method and application thereof |
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| CN201610846952.4A CN106397674B (en) | 2016-09-23 | 2016-09-23 | A kind of cellulose modified material of Thermo-sensitive Prevention of postsurgical adhesion and the preparation method and application thereof |
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| Publication Number | Publication Date |
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| CN107537059A (en) * | 2017-10-01 | 2018-01-05 | 刘云晖 | A kind of two-component solubility amphion aerogel dressing and preparation method thereof |
| CN108752530A (en) * | 2018-05-09 | 2018-11-06 | 同济大学 | It can the temperature-sensitive hydrogel materials of load cells, temperature-sensitive hydrogel and the preparation method and application thereof |
| CN109826015A (en) * | 2019-01-30 | 2019-05-31 | 广西大学 | Temperature sensitive/pH the double-bang firecracker of one kind answers intelligent nano fibrous material and its preparation method and application |
| CN109966539A (en) * | 2019-04-11 | 2019-07-05 | 中原工学院 | A kind of preparation method of low adherency wound dressing |
| CN110384831A (en) * | 2019-06-30 | 2019-10-29 | 天津大学 | Preparation method for the amphoteric ion hydrogel and crosslinking agent of post-operation adhesion preventing, polymer |
| CN114835826A (en) * | 2022-04-22 | 2022-08-02 | 深圳华源再生医学有限公司 | Zwitterionic cellulose and preparation method and application thereof |
| CN115322397A (en) * | 2022-09-23 | 2022-11-11 | 福州大学 | Zwitterionic hydrogel for preventing postoperative abdominal adhesion and preparation method thereof |
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| CN107537059A (en) * | 2017-10-01 | 2018-01-05 | 刘云晖 | A kind of two-component solubility amphion aerogel dressing and preparation method thereof |
| CN107537059B (en) * | 2017-10-01 | 2020-06-05 | 山东煜和堂药业有限公司 | Two-component soluble amphoteric ion hydrogel dressing and preparation method thereof |
| CN108752530A (en) * | 2018-05-09 | 2018-11-06 | 同济大学 | It can the temperature-sensitive hydrogel materials of load cells, temperature-sensitive hydrogel and the preparation method and application thereof |
| CN109826015A (en) * | 2019-01-30 | 2019-05-31 | 广西大学 | Temperature sensitive/pH the double-bang firecracker of one kind answers intelligent nano fibrous material and its preparation method and application |
| CN109826015B (en) * | 2019-01-30 | 2021-03-26 | 广西大学 | temperature-sensitive/pH dual-response intelligent nanofiber material and preparation method and application thereof |
| CN109966539A (en) * | 2019-04-11 | 2019-07-05 | 中原工学院 | A kind of preparation method of low adherency wound dressing |
| CN110384831A (en) * | 2019-06-30 | 2019-10-29 | 天津大学 | Preparation method for the amphoteric ion hydrogel and crosslinking agent of post-operation adhesion preventing, polymer |
| CN110384831B (en) * | 2019-06-30 | 2021-10-08 | 天津大学 | Preparation method of zwitterionic hydrogel for postoperative adhesion prevention, cross-linking agent and polymer |
| CN114835826A (en) * | 2022-04-22 | 2022-08-02 | 深圳华源再生医学有限公司 | Zwitterionic cellulose and preparation method and application thereof |
| CN114835826B (en) * | 2022-04-22 | 2023-08-22 | 深圳华源再生医学有限公司 | Zwitterionic cellulose and preparation method and application thereof |
| CN115322397A (en) * | 2022-09-23 | 2022-11-11 | 福州大学 | Zwitterionic hydrogel for preventing postoperative abdominal adhesion and preparation method thereof |
| CN115322397B (en) * | 2022-09-23 | 2024-04-30 | 福州大学 | Zwitterionic hydrogel for preventing postoperative abdominal adhesion and preparation method thereof |
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