CN106390522B - The chromatographic column of high separating effect - Google Patents
The chromatographic column of high separating effect Download PDFInfo
- Publication number
- CN106390522B CN106390522B CN201611039603.8A CN201611039603A CN106390522B CN 106390522 B CN106390522 B CN 106390522B CN 201611039603 A CN201611039603 A CN 201611039603A CN 106390522 B CN106390522 B CN 106390522B
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- Prior art keywords
- stationary phase
- column
- shell
- separating effect
- chromatographic column
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- 230000000694 effects Effects 0.000 title claims abstract description 17
- 230000005526 G1 to G0 transition Effects 0.000 claims abstract description 50
- 239000000203 mixture Substances 0.000 claims abstract description 4
- 238000001914 filtration Methods 0.000 claims description 11
- 239000012530 fluid Substances 0.000 claims description 3
- 230000013011 mating Effects 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 abstract description 4
- 238000000926 separation method Methods 0.000 abstract description 4
- 238000002360 preparation method Methods 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 5
- 239000003814 drug Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 240000006409 Acacia auriculiformis Species 0.000 description 2
- 210000005069 ears Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The invention discloses a kind of chromatographic columns of high separating effect, comprising: the shell of inner hollow；And it is disposed in the housing the stationary phase in portion, wherein the stationary phase includes that column stationary phase and the spherical stationary phase group by several spherical fixed phase compositions, the spherical stationary phase group are stacked on the column stationary phase.According to the present invention, the length dimension of chromatographic column can be controlled, to keep its stable type, reduce time and the preparation process of separation, improve the beneficial effect of separative efficiency while improving the separative efficiency of compound by having.
Description
Technical field
The present invention relates to a kind of chromatographic column, in particular to a kind of chromatographic column of high separating effect.
Background technique
Chromatographic column can be used in industrial manufacture process to carry out purification to process liquid and isolate from process liquid
Interested substance.Impurity is inevitably mixed into when preparing the chemical products such as protein and drug, and in actually using
It is very high to the purity requirement of protein and drug this substance, therefore, it is general using chromatographic column come to protein and drug etc. into
Row purifying, to obtain the product of high-purity.
It is often necessary to repeatedly purify to obtain the product of aimed purity, common way is by more different separation
Rank, type chromatographic column connect, but such method makes the serial operation between multistage chromatographic column become cumbersome, slightly
Have accidentally, easily the generation phenomenon of leakage；Too long chromatographic column needs additional fixation device after series connection, so as to cause into one
Step has aggravated the time of separation compound, greatly reduces separative efficiency.In view of this, it is really necessary to develop a kind of high score
Chromatographic column from effect, to solve the above problems.
Summary of the invention
Shortcoming present in view of the above technology, the object of the present invention is to provide a kind of chromatographies of high separating effect
Column can control the length dimension of chromatographic column while improving the separative efficiency of compound, to keep its stable type, reduce
Isolated time and preparation process improves separative efficiency.
In order to realize above-mentioned purpose according to the present invention and other advantages, a kind of chromatographic column of high separating effect is provided,
Include:
The shell of inner hollow；And
It is disposed in the housing the stationary phase in portion,
Wherein, the stationary phase includes column stationary phase and the spherical stationary phase group by several spherical fixed phase compositions, institute
Spherical stationary phase group is stated to be stacked on the column stationary phase.
Preferably, the upper surface of the column stationary phase offers coniform diversion groove.
Preferably, the apex angle of the diversion groove is located at the inside of the column stationary phase, the cone of the diversion groove
Angle is set as α, then has 30°≤α≤75°。
Preferably, the shell includes cylindrical shell and rectangle shell, the cylindrical shell and rectangle shell one
Body formula it is connected to.
Preferably, the column stationary phase is set in the cylindrical shell, and the spherical stationary phase is mounted on the length
In square casing.
Preferably, the lower part of the cylindrical shell is collapsed into the fluid hole that outer surface has mating screw thread, described rectangular
The top of shape shell, which is removably matched, is connected to lid.
Preferably, the lid is equipped with the inlet opening towards the enclosure interior.
Preferably, the first filter, the lid are equipped between the bottom of the cylindrical shell and the column stationary phase
The second filter is equipped between body and the spherical stationary phase group.
Preferably, it is equipped with several refined filtration holes inside the column stationary phase, the size in the refined filtration hole is 10~
100nm, the inside of the spherical stationary phase are equipped with several coarse filtration holes, and the size in the coarse filtration hole is 100~300nm, the ball
Mutual gap between shape stationary phase is 300~500nm.
Preferably, the outside of the shell is socketed with fixed mounting.
Compared with prior art, the present invention the beneficial effect is that: it can be while improving the separative efficiency of compound, control
The length dimension of chromatographic column processed reduces time and the preparation process of separation to keep its stable type, improves separative efficiency.
Detailed description of the invention
Fig. 1 is the three-dimensional structure diagram according to the chromatographic column of high separating effect in an embodiment of the present invention；
Fig. 2 is the cross-sectional view according to the chromatographic column of high separating effect in an embodiment of the present invention.
Specific embodiment
Present invention will be described in further detail below with reference to the accompanying drawings, foregoing end other objects of the invention, feature, side
Face and advantage will be apparent, and so that those skilled in the art can carry out according to the description.
Referring to Fig. 1, the chromatographic column 100 of high separating effect includes: that shell 110, lid 120 and fixed installation cover 130,
Wherein, 110 inner hollow of shell is equipped with stationary phase inside shell 110, if the stationary phase include column stationary phase 150 and by
The spherical stationary phase group 160 of the fixed phase composition of dry bulb shape, 160 groups of spherical stationary phase are stacked on column stationary phase 150.
Referring to Fig. 2, the upper surface of column stationary phase 150 offers coniform diversion groove, the top of the diversion groove
Angle is located at the inside of column stationary phase 150, and the cone angle of the diversion groove is set as α, then has 30 °≤α≤75 °.In an embodiment
In, α=35 °, in another embodiment, α=60 °.
Referring again to Fig. 1 and Fig. 2, shell 110 includes cylindrical shell 111 and rectangle shell 112, cylindrical shell 111 with
Rectangle shell 112 is integrally connected to, and column stationary phase 150 is set in cylindrical shell 111, and spherical stationary phase group 160 is set to
In rectangle shell 112, the lower part of cylindrical shell 11 is collapsed into the fluid hole 111a that outer surface has mating screw thread 111b, rectangular
The top of shape shell 112, which is removably matched, is connected to lid 120, and lid 120 is equipped with the inlet opening 122 led to inside shell 110.?
A circle recessing groove 121 is equipped in one embodiment, around inlet opening 122.
Referring to Fig. 2, the first filter 140, lid 120 are equipped between the bottom and column stationary phase 150 of cylindrical shell 111
It is equipped with the second filter 170 between spherical stationary phase group 160, is equipped with several refined filtration holes, the essence inside column stationary phase 150
The size of filter opening is 10~100nm, and the inside of the spherical stationary phase is equipped with several coarse filtration holes, and the size in the coarse filtration hole is
100~300nm, the mutual gap between the spherical stationary phase are 300~500nm.
Referring to Fig.1, fixed mounting 130 includes left ear 131 and auris dextra 132, and left ear 131 and auris dextra 132 are respectively equipped with peace
Fill hole 131a, 132a.
Number of devices and treatment scale described herein are for simplifying explanation of the invention.To application of the invention,
Modifications and variations will be readily apparent to persons skilled in the art.
Although the embodiments of the present invention have been disclosed as above, but it is not limited in listed fortune in specification and embodiments
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily real
Now other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is not limited to
Specific details and legend shown and described herein.
Claims (7)
1. a kind of chromatographic column of high separating effect characterized by comprising
The shell of inner hollow；And
It is disposed in the housing the stationary phase in portion,
Wherein, the stationary phase includes column stationary phase and the spherical stationary phase group by several spherical fixed phase compositions, the ball
Shape stationary phase group is stacked on the column stationary phase, and the upper surface of column stationary phase offers coniform diversion groove,
The apex angle of the diversion groove is located at the inside of the column stationary phase, and the cone angle of the diversion groove is set as α, then has 30 °≤α
≤ 75 °, the column stationary phase inside is equipped with several refined filtration holes, and the size in the refined filtration hole is 10~100nm, described spherical solid
The inside for determining phase is equipped with several coarse filtration holes, and the size in the coarse filtration hole is 100~300nm, the phase between the spherical stationary phase
Mutual gap is 300~500nm.
2. the chromatographic column of high separating effect as described in claim 1, which is characterized in that the shell includes cylindrical shell and length
Square casing, the cylindrical shell are connected to rectangle casing integrated with body.
3. the chromatographic column of high separating effect as claimed in claim 2, which is characterized in that the column stationary phase is set to the circle
In column shell, the spherical stationary phase is mounted in the rectangle shell.
4. the chromatographic column of high separating effect as claimed in claim 3, which is characterized in that the lower part of the cylindrical shell is collapsed into
Outer surface has the fluid hole of mating screw thread, and the top of the rectangle shell, which is removably matched, is connected to lid.
5. the chromatographic column of high separating effect as claimed in claim 4, which is characterized in that the lid, which is equipped with, leads to the shell
Internal inlet opening.
6. the chromatographic column of high separating effect as claimed in claim 4, which is characterized in that the bottom of the cylindrical shell with it is described
It is equipped with the first filter between column stationary phase, the second filter is equipped between the lid and the spherical stationary phase group.
7. the chromatographic column of high separating effect as described in claim 1, which is characterized in that the outside of the shell is socketed with fixation
Mounting base.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611039603.8A CN106390522B (en) | 2016-11-11 | 2016-11-11 | The chromatographic column of high separating effect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611039603.8A CN106390522B (en) | 2016-11-11 | 2016-11-11 | The chromatographic column of high separating effect |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106390522A CN106390522A (en) | 2017-02-15 |
| CN106390522B true CN106390522B (en) | 2018-12-28 |
Family
ID=58082002
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611039603.8A Active CN106390522B (en) | 2016-11-11 | 2016-11-11 | The chromatographic column of high separating effect |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106390522B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112924604B (en) * | 2019-12-06 | 2023-12-26 | 劢析科学仪器(苏州)有限公司 | Ion pair purifying column |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2687668Y (en) * | 2004-05-29 | 2005-03-23 | 周宇 | Combined chromato graphic column |
| US20050139536A1 (en) * | 2003-09-30 | 2005-06-30 | Belov Yuri P. | Multicapillary column for chromatography and sample preparation |
| CN101871920A (en) * | 2010-06-13 | 2010-10-27 | 中国环境科学研究院 | Multistage improvement column for quickly pre-processing and purifying polychlorinated biphenyl in biological sample |
| CN206198763U (en) * | 2016-11-11 | 2017-05-31 | 苏州楚博生物技术有限公司 | The chromatographic column of separating effect high |
-
2016
- 2016-11-11 CN CN201611039603.8A patent/CN106390522B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050139536A1 (en) * | 2003-09-30 | 2005-06-30 | Belov Yuri P. | Multicapillary column for chromatography and sample preparation |
| CN2687668Y (en) * | 2004-05-29 | 2005-03-23 | 周宇 | Combined chromato graphic column |
| CN101871920A (en) * | 2010-06-13 | 2010-10-27 | 中国环境科学研究院 | Multistage improvement column for quickly pre-processing and purifying polychlorinated biphenyl in biological sample |
| CN206198763U (en) * | 2016-11-11 | 2017-05-31 | 苏州楚博生物技术有限公司 | The chromatographic column of separating effect high |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106390522A (en) | 2017-02-15 |
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Address after: 436032 Gedian Development Zone, Ezhou City, Hubei Province, China, Gaoxin Third Road, Optics Valley United Technology City, Zone 206A Patentee after: Hubei Chubo Biotechnology Co.,Ltd. Country or region after: China Address before: 215000 Building 412, No. 18, Jinfeng Road, Suzhou High-tech Zone, Jiangsu Province Patentee before: SUZHOU CHUBO BIOTECHNOLOGY CO.,LTD. Country or region before: China |
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Effective date of registration: 20240401 Address after: Room B212, Incubation Site, No. 59 Mumu Street, Xiangfang District, Harbin City, Heilongjiang Province, 150038 Patentee after: Heilongjiang moseidong Technology Co.,Ltd. Country or region after: China Address before: 436032 Gedian Development Zone, Ezhou City, Hubei Province, China, Gaoxin Third Road, Optics Valley United Technology City, Zone 206A Patentee before: Hubei Chubo Biotechnology Co.,Ltd. Country or region before: China |
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