CN106383193A - Detection method for phenylethanolamine in pork - Google Patents
Detection method for phenylethanolamine in pork Download PDFInfo
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- CN106383193A CN106383193A CN201611025753.3A CN201611025753A CN106383193A CN 106383193 A CN106383193 A CN 106383193A CN 201611025753 A CN201611025753 A CN 201611025753A CN 106383193 A CN106383193 A CN 106383193A
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- phenolethanolamine
- solution
- pork
- detection method
- performance liquid
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
Abstract
The invention discloses a detection method for phenylethanolamine in pork. The detection method comprises the following steps: performing sufficient homogenizing, ultrasonic extraction and centrifuging on smashed pork at high speed by an aqueous solution of ammonium acetate and an acetonitrile and ethanol mixed solution; taking supernate, and repeating once to obtain an extracting solution; adding normal hexane, uniformly mixing and then centrifuging to obtain clear liquid on the lower layer; performing filter membrane filtering and nitrogen blowing, adding dimethyl sulfoxide, and then fixing volume by using the aqueous solution of the ammonium acetate to obtain a to-be-detected solution; detecting the to-be-detected solution by using an ultrahigh performance liquid chromatography-tandem mass spectrometry to obtain an ultrahigh performance liquid chromatogram of the to-be-detected solution; drawing a standard curve; calculating the content of the phenylethanolamine in the pork according to the peak area of the phenylethanolamine in the ultrahigh performance liquid chromatogram of the to-be-detected solution and the standard curve of a standard substance corresponding to the phenylethanolamine. According to the detection method disclosed by the invention, pretreatment analysis of a sample is simple and feasible; purification modes such as solid-phase extraction are not needed; expensive cost consumption is reduced while the detection efficiency is greatly improved, and quick and accurate detection is realized.
Description
Technical field
The present invention relates to technical field of food detection, the detection method of phenolethanolamine in specifically a kind of pork.
Background technology
Phenolethanolamine, high toxic material, flammable high, storehouse ventilation low temperature drying accumulating.Irrational use is easily made
Become medicament residue, simultaneously left drug health may be caused potentially hazardous, so must be discontinued to ensure to move on time
Physical property Product quality and safety, simultaneously according to the regulation of veterinary drug national standard and professional standard, enters to phenolethanolamine in animal tissue
Row detection and analysis.But because the physicochemical properties of various veterinary drugs are different, differ greatly, add other compositions in detection sample
Interference, to phenolethanolamine residual in animal tissue, quick analysis detection constitutes very big difficulty simultaneously.Phenolethanolamine does not have at present
There is national standard method, it is also desirable to be detected to these compounds using multiple different methods in existing national standard method,
Operating procedure is complicated, needs using solid-phase extraction column etc., and testing cost is high it is therefore desirable to a kind of general detection method of exploitation is used
Detection in daily sample.
Content of the invention
It is an object of the invention to provide a kind of simple, greatly improve detection efficiency, reduces cost, quick and precisely examine
The detection method of phenolethanolamine in the pork surveyed, to solve the problems, such as to propose in above-mentioned background technology.
For achieving the above object, the present invention provides following technical scheme：
In a kind of pork, the detection method of phenolethanolamine, comprises the following steps：
（1）Weigh the pork after 2-4g smashs to pieces in centrifuge tube, add 3-5mL ammonium acetate solution, 4-7mL acetonitrile+ethanol mixes
Close liquid, be fully homogenized at a high speed, ultrasonic extraction 4-10min after mixing, centrifugation, take supernatant A, residual residue adds 6-10mL acetonitrile
+ alcohol mixeding liquid, ultrasonic extraction 4-10min, it is centrifuged, takes supernatant B to merge with supernatant A, obtain extract, just adding 6-10mL
Hexane, is centrifuged after mixing, obtains subnatant body, take wherein 3-6mL, through membrane filtration in centrifuge tube with a scale, 40 DEG C of water
The lower nitrogen of bath is blown to 1-1.2mL, after adding 200 μ L dimethyl sulfoxide (DMSO)s, is settled to 1.5mL graduation mark with ammonium acetate solution, obtains
Solution to be measured；
（2）By step（1）The solution to be measured of gained, through Ultra Performance Liquid Chromatography-tandem mass spectrum combined instrument detection, obtains solution to be measured
Ultra Performance Liquid Chromatography figure, chromatographic condition is：With carbon octadecylsilane chemically bonded silica as filler, particle diameter≤5 μm；Gradient
Wash-out：Mobile phase A is the methanol solution of the formic acid containing volume fraction 0.1%, and Mobile phase B is the formic acid containing volume fraction 0.1%
The aqueous solution；Chromatogram column temperature is 40 DEG C；Sample size 10 μ L, flow velocity is 0.3 μ L/min；Mass Spectrometry Conditions are：ESI negative ions are simultaneously
Scan pattern, capillary voltage is respectively 3.5kV and 3.0kV, 145 DEG C of source temperature, goes solvent gas temperature：450℃；Remove solvent gas nitrogen
Gas velocity：900L/h；Taper hole gas nitrogen flow rate：50L/h；Collision gas：Argon gas 3.3 × 10-3mba；Multiple-reaction monitoring pattern is examined
Survey；
（3）Draw calibration curve：Take the standard items of phenolethanolamine, be configured to the standard working solution of the mixing of variable concentrations, according to
Step（2）The same terms are tested, and obtain the Ultra Performance Liquid Chromatography figure of standard working solution, to phenolethanolamine standard items, according to
Its concentration and peak area, draw calibration curve, obtain the calibration curve of phenolethanolamine standard items；
（4）According to step（2）In the peak area of Ultra Performance Liquid Chromatography in figure phenolethanolamine of solution to be measured and its corresponding mark
The calibration curve of quasi- product, calculates the content of phenolethanolamine in pork.
As the further scheme of the present invention：The volume ratio of acetonitrile+alcohol mixeding liquid is 3-5：1.
As the further scheme of the present invention：The concentration of ammonium acetate solution is 1-4mmol/L.
As the further scheme of the present invention：Ultrasonic power is 400-1000W.
As the further scheme of the present invention：Fully homogenate is the rotating speed high-speed homogenization with 10000-20000r/min at a high speed
1-5min.
As the further scheme of the present invention：Filter membrane is 0.22 μm of filter membrane.
Compared with prior art, the invention has the beneficial effects as follows：
Inventive samples pre-treatment analysis simple it is not necessary to the purification style such as SPE, greatly improving detection efficiency
While reduce costliness cost consumption, can be used for the detection of phenolethanolamine, disposably quick and precisely detect, expand we
The range of application of method.
Specific embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described,
Obviously, described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.Based in the present invention
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under the premise of not making creative work, all
Belong to the scope of protection of the invention.
Embodiment 1
In the embodiment of the present invention, in a kind of pork, the detection method of phenolethanolamine, comprises the following steps：
（1）Weigh the pork after 2g smashs to pieces in centrifuge tube, add 3mL 1mmol/L ammonium acetate solution, 4mL volume ratio is 3：
1 acetonitrile+alcohol mixeding liquid, with the rotating speed high-speed homogenization 1min of 10000r/min, ultrasonic extraction 4min after fully homogenate mixes,
Centrifugation, takes supernatant A, and it is 3 that residual residue adds 6mL volume ratio：1 acetonitrile+alcohol mixeding liquid, ultrasonic extraction 4min, ultrasonic
Power is 400W.It is centrifuged, takes supernatant B to merge with supernatant A, obtain extract, add 6mL n-hexane, be centrifuged after mixing, obtain down
Layer clear liquid body, takes wherein 3mL, through 0.22 μm of membrane filtration in centrifuge tube with a scale, under 40 DEG C of water-baths, nitrogen is blown to 1mL,
After adding 200 μ L dimethyl sulfoxide (DMSO)s, it is settled to 1.5mL graduation mark with 1mmol/L ammonium acetate solution, obtains solution to be measured.
（2）By step（1）The solution to be measured of gained, through Ultra Performance Liquid Chromatography-tandem mass spectrum combined instrument detection, obtains to be measured
The Ultra Performance Liquid Chromatography figure of solution, chromatographic condition is：With carbon octadecylsilane chemically bonded silica as filler, particle diameter≤5 μm；
Gradient elution：Mobile phase A is the methanol solution of the formic acid containing volume fraction 0.1%, and Mobile phase B is the first containing volume fraction 0.1%
Aqueous acid；Chromatogram column temperature is 40 DEG C；Sample size 10 μ L, flow velocity is 0.3 μ L/min；Mass Spectrometry Conditions are：ESI negative ions
Scan pattern simultaneously, capillary voltage is respectively 3.5kV and 3.0kV, 145 DEG C of source temperature, goes solvent gas temperature：450℃；Remove solvent
Gas nitrogen flow rate：900L/h；Taper hole gas nitrogen flow rate：50L/h；Collision gas：Argon gas 3.3 × 10-3mba；Multiple-reaction monitoring mould
Formula detects.
（3）Draw calibration curve：Take the standard items of phenolethanolamine, be configured to the standard working solution of the mixing of variable concentrations,
According to step（2）The same terms are tested, and obtain the Ultra Performance Liquid Chromatography figure of standard working solution, to phenolethanolamine standard items,
According to its concentration and peak area, draw calibration curve, obtain the calibration curve of phenolethanolamine standard items.
（4）According to step（2）In the peak area of Ultra Performance Liquid Chromatography in figure phenolethanolamine of solution to be measured and its correspondence
Standard items calibration curve, calculate the content of phenolethanolamine in pork.
Embodiment 2
In the embodiment of the present invention, in a kind of pork, the detection method of phenolethanolamine, comprises the following steps：
（1）Weigh the pork after 2-4g smashs to pieces in centrifuge tube, add 5mL 4mmol/L ammonium acetate solution, 7mL volume ratio is
5：1 acetonitrile+alcohol mixeding liquid, with the rotating speed high-speed homogenization 5min of 20000r/min, ultrasonic extraction after fully homogenate mixes
10min, centrifugation, take supernatant A, it is 5 that residual residue adds 10mL volume ratio：1 acetonitrile+alcohol mixeding liquid, ultrasonic extraction
10min, ultrasonic power is 1000W.It is centrifuged, takes supernatant B to merge with supernatant A, obtain extract, add 10mL n-hexane, mix
It is centrifuged after even, obtain subnatant body, take wherein 6mL, through 0.22 μm of membrane filtration in centrifuge tube with a scale, under 40 DEG C of water-baths
Nitrogen is blown to 1.2mL, after adding 200 μ L dimethyl sulfoxide (DMSO)s, is settled to 1.5mL graduation mark with 4mmol/L ammonium acetate solution, obtains
Solution to be measured.
（2）By step（1）The solution to be measured of gained, through Ultra Performance Liquid Chromatography-tandem mass spectrum combined instrument detection, obtains to be measured
The Ultra Performance Liquid Chromatography figure of solution, chromatographic condition is：With carbon octadecylsilane chemically bonded silica as filler, particle diameter≤5 μm；
Gradient elution：Mobile phase A is the methanol solution of the formic acid containing volume fraction 0.1%, and Mobile phase B is the first containing volume fraction 0.1%
Aqueous acid；Chromatogram column temperature is 40 DEG C；Sample size 10 μ L, flow velocity is 0.3 μ L/min；Mass Spectrometry Conditions are：ESI negative ions
Scan pattern simultaneously, capillary voltage is respectively 3.5kV and 3.0kV, 145 DEG C of source temperature, goes solvent gas temperature：450℃；Remove solvent
Gas nitrogen flow rate：900L/h；Taper hole gas nitrogen flow rate：50L/h；Collision gas：Argon gas 3.3 × 10-3mba；Multiple-reaction monitoring mould
Formula detects.
（3）Draw calibration curve：Take the standard items of phenolethanolamine, be configured to the standard working solution of the mixing of variable concentrations,
According to step（2）The same terms are tested, and obtain the Ultra Performance Liquid Chromatography figure of standard working solution, to phenolethanolamine standard items,
According to its concentration and peak area, draw calibration curve, obtain the calibration curve of phenolethanolamine standard items.
（4）According to step（2）In the peak area of Ultra Performance Liquid Chromatography in figure phenolethanolamine of solution to be measured and its correspondence
Standard items calibration curve, calculate the content of phenolethanolamine in pork.
Embodiment 3
In the embodiment of the present invention, in a kind of pork, the detection method of phenolethanolamine, comprises the following steps：
（1）Weigh the pork after 2-4g smashs to pieces in centrifuge tube, add 4mL 2mmol/L ammonium acetate solution, 5mL volume ratio is
4：1 acetonitrile+alcohol mixeding liquid, with the rotating speed high-speed homogenization 3min of 20000r/min, ultrasonic extraction after fully homogenate mixes
8min, centrifugation, take supernatant A, it is 4 that residual residue adds 8mL volume ratio：1 acetonitrile+alcohol mixeding liquid, ultrasonic extraction 8min,
Ultrasonic power is 800W.It is centrifuged, takes supernatant B to merge with supernatant A, obtain extract, add 8mL n-hexane, be centrifuged after mixing,
Obtain subnatant body, take wherein 5mL, through 0.22 μm of membrane filtration in centrifuge tube with a scale, under 40 DEG C of water-baths, nitrogen is blown to
1.1mL, after adding 200 μ L dimethyl sulfoxide (DMSO)s, is settled to 1.5mL graduation mark with 2mmol/L ammonium acetate solution, obtains to be measured molten
Liquid.
（2）By step（1）The solution to be measured of gained, through Ultra Performance Liquid Chromatography-tandem mass spectrum combined instrument detection, obtains to be measured
The Ultra Performance Liquid Chromatography figure of solution, chromatographic condition is：With carbon octadecylsilane chemically bonded silica as filler, particle diameter≤5 μm；
Gradient elution：Mobile phase A is the methanol solution of the formic acid containing volume fraction 0.1%, and Mobile phase B is the first containing volume fraction 0.1%
Aqueous acid；Chromatogram column temperature is 40 DEG C；Sample size 10 μ L, flow velocity is 0.3 μ L/min；Mass Spectrometry Conditions are：ESI negative ions
Scan pattern simultaneously, capillary voltage is respectively 3.5kV and 3.0kV, 145 DEG C of source temperature, goes solvent gas temperature：450℃；Remove solvent
Gas nitrogen flow rate：900L/h；Taper hole gas nitrogen flow rate：50L/h；Collision gas：Argon gas 3.3 × 10-3mba；Multiple-reaction monitoring mould
Formula detects.
（3）Draw calibration curve：Take the standard items of phenolethanolamine, be configured to the standard working solution of the mixing of variable concentrations,
According to step（2）The same terms are tested, and obtain the Ultra Performance Liquid Chromatography figure of standard working solution, to phenolethanolamine standard items,
According to its concentration and peak area, draw calibration curve, obtain the calibration curve of phenolethanolamine standard items.
（4）According to step（2）In the peak area of Ultra Performance Liquid Chromatography in figure phenolethanolamine of solution to be measured and its correspondence
Standard items calibration curve, calculate the content of phenolethanolamine in pork.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of the spirit or essential attributes of the present invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although this specification is been described by according to embodiment, not each embodiment only wraps
Containing an independent technical scheme, only for clarity, those skilled in the art should for this narrating mode of specification
Using specification as an entirety, the technical scheme in each embodiment can also form those skilled in the art through appropriately combined
Understandable other embodiment.
Claims (6)
1. in a kind of pork the detection method of phenolethanolamine it is characterised in that comprising the following steps：
（1）Weigh the pork after 2-4g smashs to pieces in centrifuge tube, add 3-5mL ammonium acetate solution, 4-7mL acetonitrile+ethanol mixes
Close liquid, be fully homogenized at a high speed, ultrasonic extraction 4-10min after mixing, centrifugation, take supernatant A, residual residue adds 6-10mL acetonitrile
+ alcohol mixeding liquid, ultrasonic extraction 4-10min, it is centrifuged, takes supernatant B to merge with supernatant A, obtain extract, just adding 6-10mL
Hexane, is centrifuged after mixing, obtains subnatant body, take wherein 3-6mL, through membrane filtration in centrifuge tube with a scale, 40 DEG C of water
The lower nitrogen of bath is blown to 1-1.2mL, after adding 200 μ L dimethyl sulfoxide (DMSO)s, is settled to 1.5mL graduation mark with ammonium acetate solution, obtains
Solution to be measured；
（2）By step（1）The solution to be measured of gained, through Ultra Performance Liquid Chromatography-tandem mass spectrum combined instrument detection, obtains solution to be measured
Ultra Performance Liquid Chromatography figure, chromatographic condition is：With carbon octadecylsilane chemically bonded silica as filler, particle diameter≤5 μm；Gradient
Wash-out：Mobile phase A is the methanol solution of the formic acid containing volume fraction 0.1%, and Mobile phase B is the formic acid containing volume fraction 0.1%
The aqueous solution；Chromatogram column temperature is 40 DEG C；Sample size 10 μ L, flow velocity is 0.3 μ L/min；Mass Spectrometry Conditions are：ESI negative ions are simultaneously
Scan pattern, capillary voltage is respectively 3.5kV and 3.0kV, 145 DEG C of source temperature, goes solvent gas temperature：450℃；Remove solvent gas nitrogen
Gas velocity：900L/h；Taper hole gas nitrogen flow rate：50L/h；Collision gas：Argon gas 3.3 × 10-3mba；Multiple-reaction monitoring pattern is examined
Survey；
（3）Draw calibration curve：Take the standard items of phenolethanolamine, be configured to the standard working solution of the mixing of variable concentrations, according to
Step（2）The same terms are tested, and obtain the Ultra Performance Liquid Chromatography figure of standard working solution, to phenolethanolamine standard items, according to
Its concentration and peak area, draw calibration curve, obtain the calibration curve of phenolethanolamine standard items；
（4）According to step（2）In the peak area of Ultra Performance Liquid Chromatography in figure phenolethanolamine of solution to be measured and its corresponding mark
The calibration curve of quasi- product, calculates the content of phenolethanolamine in pork.
2. in pork according to claim 1 the detection method of phenolethanolamine it is characterised in that acetonitrile+alcohol mixeding liquid
Volume ratio be 3-5：1.
3. in pork according to claim 1 phenolethanolamine detection method it is characterised in that ammonium acetate solution dense
Spend for 1-4mmol/L.
4. in pork according to claim 1 phenolethanolamine detection method it is characterised in that ultrasonic power be 400-
1000W.
5. in pork according to claim 1 phenolethanolamine detection method it is characterised in that at a high speed fully homogenate be with
The rotating speed high-speed homogenization 1-5min of 10000-20000r/min.
6. in pork according to claim 1 phenolethanolamine detection method it is characterised in that filter membrane be 0.22 μm filter
Film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201611025753.3A CN106383193A (en) | 2016-11-22 | 2016-11-22 | Detection method for phenylethanolamine in pork |
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| CN201611025753.3A CN106383193A (en) | 2016-11-22 | 2016-11-22 | Detection method for phenylethanolamine in pork |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111175403A (en) * | 2020-01-19 | 2020-05-19 | 山东中质华检测试检验有限公司 | Method for detecting histamine in aquatic product |
| CN113624853A (en) * | 2020-05-07 | 2021-11-09 | 厦门泓益检测有限公司 | Method for simultaneously detecting cathartic components in weight-reducing product based on UPLC-MS/MS |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5357191A (en) * | 1976-11-04 | 1978-05-24 | Asahi Chem Ind Co Ltd | Activated carbon for adsorption of toxin in serum |
| WO1999064861A2 (en) * | 1998-06-11 | 1999-12-16 | University College London | Method of identifying antidepressant compounds |
| WO2011028987A2 (en) * | 2009-09-03 | 2011-03-10 | University Of Tennessee Research Foundation | Methods of improving nutritional value of plants |
| CN103713056A (en) * | 2013-11-25 | 2014-04-09 | 宁波出入境检验检疫局检验检疫技术中心 | Method for simultaneously analyzing and detecting residual veterinary drug compositions in animal tissue |
| CN104297233A (en) * | 2014-09-28 | 2015-01-21 | 中国农业大学 | Detection method and kit for detecting beta-receptor stimulant drugs in animal body fluid |
| CN104764844A (en) * | 2015-03-18 | 2015-07-08 | 谱尼测试科技股份有限公司 | Detection method of measuring residue amount of 35 veterinary drugs in meat of poultry and livestock |
| CN104977367A (en) * | 2014-04-01 | 2015-10-14 | 中粮营养健康研究院有限公司 | Method for detecting 20 kinds of beta-stimulant drug residues in livestock urine |
| CN105784857A (en) * | 2015-11-27 | 2016-07-20 | 佛山科学技术学院 | Analysis test method for ractopamine |
-
2016
- 2016-11-22 CN CN201611025753.3A patent/CN106383193A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5357191A (en) * | 1976-11-04 | 1978-05-24 | Asahi Chem Ind Co Ltd | Activated carbon for adsorption of toxin in serum |
| WO1999064861A2 (en) * | 1998-06-11 | 1999-12-16 | University College London | Method of identifying antidepressant compounds |
| WO2011028987A2 (en) * | 2009-09-03 | 2011-03-10 | University Of Tennessee Research Foundation | Methods of improving nutritional value of plants |
| CN103713056A (en) * | 2013-11-25 | 2014-04-09 | 宁波出入境检验检疫局检验检疫技术中心 | Method for simultaneously analyzing and detecting residual veterinary drug compositions in animal tissue |
| CN104977367A (en) * | 2014-04-01 | 2015-10-14 | 中粮营养健康研究院有限公司 | Method for detecting 20 kinds of beta-stimulant drug residues in livestock urine |
| CN104297233A (en) * | 2014-09-28 | 2015-01-21 | 中国农业大学 | Detection method and kit for detecting beta-receptor stimulant drugs in animal body fluid |
| CN104764844A (en) * | 2015-03-18 | 2015-07-08 | 谱尼测试科技股份有限公司 | Detection method of measuring residue amount of 35 veterinary drugs in meat of poultry and livestock |
| CN105784857A (en) * | 2015-11-27 | 2016-07-20 | 佛山科学技术学院 | Analysis test method for ractopamine |
Non-Patent Citations (3)
| Title |
|---|
| KE-JING HUANG 等: "Ultrasound-assisted dispersive liquid–liquid microextraction combined with high-performance liquid chromatography-fluorescence detection for sensitive determination of biogenic amines in rice wine samples", 《JOURNAL OF CHROMATOGRAPHY A》 * |
| 湛嘉: "动物源性食品中多类别有机小分子污染物的快速筛查技术的研究", 《万方学位论文数据库》 * |
| 王瑞国 等: "超高效液相色谱法测定饲料中苯乙醇胺 A", 《分析化学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111175403A (en) * | 2020-01-19 | 2020-05-19 | 山东中质华检测试检验有限公司 | Method for detecting histamine in aquatic product |
| CN113624853A (en) * | 2020-05-07 | 2021-11-09 | 厦门泓益检测有限公司 | Method for simultaneously detecting cathartic components in weight-reducing product based on UPLC-MS/MS |
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Application publication date: 20170208 |