CN106378930A - Photocuring 3D printing method for thermo-sensitivity cultivated surface material - Google Patents
Photocuring 3D printing method for thermo-sensitivity cultivated surface material Download PDFInfo
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- CN106378930A CN106378930A CN201610834643.5A CN201610834643A CN106378930A CN 106378930 A CN106378930 A CN 106378930A CN 201610834643 A CN201610834643 A CN 201610834643A CN 106378930 A CN106378930 A CN 106378930A
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- temperature sensitive
- photocuring
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- culture surface
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y30/00—Apparatus for additive manufacturing; Details thereof or accessories therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/10—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration
- B08B3/12—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration by sonic or ultrasonic vibrations
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- Engineering & Computer Science (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The invention discloses a photocuring 3D printing method for a thermo-sensitivity cultivated surface material. The method comprises the following steps that firstly, a photocuring 3D printing thermo-sensitivity material is prepared; secondly, the thermo-sensitivity printing material is jetted and printed on the surface of a base material through a photocuring 3D printer, and the ultraviolet or laser irradiation grafted curing reaction is conducted; thirdly, the thermo-sensitivity printing material and the base material are subjected to further grated curing in a transmission belt type constant-temperature heating channel; and fourthly, the prepared thermo-sensitivity cultivated surface product is washed through ultrapure water and dried. The thickness of the obtained thermo-sensitivity cultivated surface is more uniform and consistent, product quality control is more convenient, equipment cost is low, scale preparing repeatability is better, and the photocuring 3D printing method is easier to achieve.
Description
Technical field
The present invention relates to photocuring 3D printing technique field, more specifically, it is to be related to a kind of temperature sensitive culture surface material
Photocuring 3D printing preparation method.
Background technology
With the continuous progress of mankind's scientific and technological level, the achievement in temperature-responsive investigation of materials field also becomes increasingly abundant.Its
In, by the research opinion in the fields such as temperature-responsive materials application to cell and tissue culture, medicament slow release, Protein Separation and purification
Literary composition and patent report are also increasing in nearest decades.In biological, medicine and medical domain, these temperature-responsive materials
The middle research report belonging to NIPA class temperature sensitive polymer is at most.This kind of temperature sensing material has a structure to become with temperature
The characteristic changed and change, thus lead to the hydrophilic-hydrophobic performance of material to change.Its hydrophilic and hydrophobic changes has one to face
Boundary's transition temperature（LCST）, higher than this temperature, material shows as hydrophobic；Less than this temperature, material shows as hydrophilic.Thin
Born of the same parents' biology and tissue engineering field, are sent out with the change of ambient temperature using NIPA class temperature sensitive polymer
The not damaged that the characteristic of raw hydrophilic and hydrophobic sex reversal can realize cell under conditions of outer need not adding proteolytic enzyme is received
Obtain or the not damaged of tissue lamellar shifts, thus reducing the toxigenic capacity of cell and tissue lamellar and improving harvesting and tissue
The perfect degree of lamella and function.
At present in the document and patent of report, the preparation method on this temperature sensitive surface for carrying out cell and tissue culture
In, great majority are all using first NIPA class monomer being configured to certain density temperature sensitive solution, or then
This temperature sensitive solution is adopted hardened coating in the substrate surface of graft polymerization preformer, or the substrate surface of graft polymerization preformer is immersed in temperature sensitive liquid
Further take out after middle certain time, finally carry out high energy electron beams and carry out graft reaction.Temperature sensitive culture surface is prepared in this
There are a lot of shortcomings in method, being that high energy electron beam device is extremely expensive first makes it be difficult to popularize, and also hardened coating can make temperature
Quick material distribution makes the method poor reproducibility with uneven thickness, furthermore the electron beam irradiation of high dose can make the base material of graft polymerization preformer
Color change, mechanical property is occurred to reduce and so that final product serviceability is deteriorated.
In existing patent and document report, also there is little researcher using adding photosensitizer in temperature sensitive liquid, so
Carry out the temperature sensitive liquid coating of substrate surface afterwards, finally prepare temperature sensitive culture surface with UV illumination initiation grafting polyreaction
Method.This method is equally to there is a problem of that temperature sensitive polymer layer is distributed and in uneven thickness, and by single ultraviolet
Light radiation polymerization mode, temperature sensitive monomer is difficult to reaction thoroughly.Additionally, some researcher is first in the base material table of graft polymerization preformer
Face introduces the group of halogen atom, then causes temperature sensitive monomer to carry out fixed point polymerization using atom transfer radical polymerization principle,
Thus realizing the temperature-sensing property of culture surface.Although the finally prepd temperature sensitive superficiality of the preparation method of this kind of temperature sensitive culture surface
Can ratio more uniform, but technics comparing is complicated, preparation cost is higher, and grafting amount is low, thus be difficult to batch prepare.
3D printing technique is to first pass through Computerized three-dimensional software to carry out three-dimensional graphics design to pre-prepared product, then will
3-D graphic information is converted into 3D printing code and is entered in 3D printer, is superimposed by the printing layer by layer of printer
Form the intelligent Manufacturing Technology of entity afterwards.In all of 3D printing technique, it is fastest that photocuring 3D printing technique manufactures,
And printing precision is high, favorable reproducibility, thickness error can control in positive and negative 0.001～0.002mm, is a kind of most useful for scale
The intelligent Manufacturing Technology changed.But photocuring 3D printing technique is applied in the preparation of temperature sensitive culture surface and yet there are no document
And patent report.
Content of the invention
It is an object of the invention to provide a kind of photocuring 3D printing preparation method of temperature sensitive culture surface material, to overcome
Deficiency in the presence of prior art.
The technical scheme that the present invention solves its technical problem is：A kind of photocuring 3D printing side of temperature sensitive culture surface material
Method, comprises the following steps：S1）Prepare photocuring 3D printing temperature sensing material；S2）Using photocuring 3D printer by temperature sensitive printing material
Material jet printing on substrate surface and carries out ultraviolet or laser irradiation grafting curing reaction；S3）In band type heated at constant temperature
Itself and the further graft curing of base material is made in passage；S4）The temperature sensitive culture surface product utilization ultra-pure water of preparation is carried out,
Dry.
Described substrate surface refer to surface treated be condensed can containing abundant hydroxyl or carboxyl and with silane
The glass of reaction, pottery or frosting.
Described photocuring 3D printer refers to Ployjet class ejection-type photocuring 3D printer, and the light source of radiation curing is adopted
The layer thickness control of the temperature sensitive polymer film being solidified with ultraviolet or LASER Light Source, printing, between 0.01～0.05mm, vertically prints
Speed controlling is in 0.1～0.3mm/min.
In S4 step, temperature sensitive culture surface product adopts soaking and washing and the combined type cleaning process being cleaned by ultrasonic or list
Solely using ultrasonic cleaning.
The soaking and washing time is 8～72h.
The concussion frequency being cleaned by ultrasonic is 20～150KHz, and the time is 5～60min.
Described photocuring 3D printing temperature sensing material includes temperature sensitive monomer, grafted monomers, cross-linking agent, chemical initiator, photosensitive
Initiator and solvent, wherein, the molar ratio of temperature sensitive monomer and grafted monomers is 95:5～85:In the range of 15, cross-linking agent is temperature
Quick monomer and the 0.5%～1% of grafted monomers total moles quantity, chemical initiator is temperature sensitive monomer and grafted monomers total moles quantity
0.5%～1%, photoinitiator is the 0.5%～2% of temperature sensitive monomer and grafted monomers total moles quantity.
Described temperature sensitive monomer includes NIPA or N- isopropyl -2- Methacrylamide, or N- isopropyl
Acrylamide and the blend of N- isopropyl -2- Methacrylamide, or NIPA and N- isopropyl -2- methyl
The oligomer of acrylamide, or it is less than 2000 NIPA and N- isopropyl -2- methacryl by molecular weight
The blend of the oligomer mixing gained of amine.
Described grafted monomers include the silane coupling agent containing unsaturated double-bond, allyltriethoxysilane, allyl
Base trimethoxy silane, 2- methacrylic triethoxysilane, 2- methacrylic trimethoxy silane, propyl acrylate-
3- trimethoxy silane, propyl acrylate -3- triethoxysilane, propyl methacrylate -3- trimethoxy silane, methyl-prop
The one of which of olefin(e) acid propyl ester -3- triethoxysilane or any two kinds or two or more mixture.
Described cross-linking agent includes diacrylate-BDO ester, Ethylene glycol dimethacrylate, N, N- di-2-ethylhexylphosphine oxide
Acrylimide, bismaleimide amine material, described cross-linking agent contains two or more unsaturated double-bonds；Described chemistry
Initiator includes azodiisobutyronitrile, 2,2'-Azobis(2,4-dimethylvaleronitrile) or di-t-butyl peroxide, benzoyl peroxide, peroxidating diisopropyl
Benzene, potassium peroxydisulfate, Ammonium persulfate.；Described photoinitiator using can under light illumination Direct Resolution produce free radical or anion,
The benzoin and its derivatives of cation or acetophenones derivant or benzophenone and its derivant；Described solvent is polarity
Aprotic solvent, including DMF, N,N-dimethylacetamide, N-Methyl pyrrolidone, oxolane, ring
The one or more of which of hexanone, described solvent is not low toxicity compounds with hydroxyl and carboxyl reaction.
The invention has the beneficial effects as follows：
The principle of the present invention is using photocuring 3D printing intelligent Manufacturing Technology by the temperature sensing material preparing in advance compositionss first
It is injected in the substrate surface of graft polymerization preformer, then use ultraviolet or laser irradiation to cause temperature sensitive rmc monomer graft curing, then heat
Carry out graft curing reaction further so as to reaction is more thorough, temperature sensitive polymer graft reaction is more perfect, be finally carried out with
Remove the small-molecule substance that temperature sensitive culture surface may remain.
The invention has the beneficial effects as follows the temperature sensitive culture surface thickness using the preparation of photocuring 3D printing technique is more uniform
Unanimously, material temperature sensitive with the hardened coating of existing document and patent report and then product of being more convenient for compared with carrying out electron beam irradiation
Quality control, equipment cost is cheap, and prepare with scale repeatability is more preferably it is easier to realize.Therefore, the present invention prepares temperature sensitive culture
The market prospect of surface product will be more wide.
Brief description
Fig. 1 is the temperature sensitive culture surface material 3T3 cell culture picture of 3D printing.
Fig. 2 is that the temperature sensitive culture surface material of 3D printing carries out picture after cell detachment for the 3T3 cell culture test.
Specific embodiment
Embodiments of the invention are described below in detail it is noted that embodiment described in the invention is exemplary, only
For explaining the present invention, and it is not considered as limiting the invention.
Embodiment 1
The present embodiment prepares a kind of temperature sensitive culture surface product, and detailed preparation process is as follows：
According to mol ratio 95:5 weigh NIPA and allyltriethoxysilane, then weigh molal quantity respectively
For the 0.5% of the above two sums N,N methylene bis acrylamide, benzoyl peroxide, Benzoinum, it is eventually adding as gross mass
As solvent, stirring makes it fully dissolve to the oxolane of fraction 40%, stand-by with 300 mesh sieve net filtrations.
Silicate glass platelet substrate material is soaked respectively in sodium hydrate aqueous solution and hydrogen peroxide aqueous sulfuric acid
Process, dry.The temperature sensing material above preparing mixed solution is added to the resin liquid pool of ejection-type photocuring 3D printer
In, input the print code got well and changed with computer 3D software design in advance（In this embodiment, the temperature sensitive thickness of design printing is
10um）.Previously processed glass flake base material is placed on the printing bed of 3D printer, opens 3D printer and start to beat
Print.After 3D printer printing terminates, the glass flake printing temperature sensitive layer is put at heating in 100 DEG C of heated constant temperature passage
Reason 20min makes its further graft curing.After having processed, put it into soaking and washing 48h in RO water, super under 50KHz frequency
Sound cleans 5 minutes, and the contact angle then drying the quick culture surface of thermometric and water at 25 DEG C is 68o, and send to gamma radiation sterilization
After carry out 3T3 cell culture test.Cell culture condition is：5% CO2, basal medium containing 10% hyclone, 37 DEG C
48h is cultivated in incubator（As shown in Figure 1）, then under the conditions of 20 DEG C, place 20min, gently blown and beaten under 15 with pasteur pipet,
Cell can all come off in flakes（As shown in Figure 2）.
Embodiment 2
The present embodiment prepares a kind of temperature sensitive culture surface product, and detailed preparation process is as follows：
According to mol ratio 95:5 weigh NIPA and allyltriethoxysilane, then weigh molal quantity respectively
For the 0.8% of the above two sums N,N methylene bis acrylamide, benzoyl peroxide, Benzoinum, it is eventually adding as gross mass
As solvent, stirring makes it fully dissolve to the oxolane of fraction 40%, stand-by with 300 mesh sieve net filtrations.
Silicate glass platelet substrate material is soaked respectively in sodium hydrate aqueous solution and hydrogen peroxide aqueous sulfuric acid
Process, dry.The temperature sensing material above preparing mixed solution is added to the resin liquid pool of ejection-type photocuring 3D printer
In, input the print code got well and changed with computer 3D software design in advance（In this embodiment, the temperature sensitive thickness of design printing is
10um）.Previously processed glass flake base material is placed on the printing bed of 3D printer, opens 3D printer and start to beat
Print.After 3D printer printing terminates, the glass flake printing temperature sensitive layer is put at heating in 100 DEG C of heated constant temperature passage
Reason 20min makes its further graft curing.After having processed, put it into soaking and washing 48h in RO water, super under 50KHz frequency
Sound cleans 5 minutes, and the contact angle then drying the quick culture surface of thermometric and water at 25 DEG C is 70o, and send to gamma radiation sterilization
After carry out 3T3 cell culture test.Cell culture condition is：5% CO2, basal medium containing 10% hyclone, 37 DEG C
Place 20min under the conditions of 20 DEG C after culture 48h in incubator, gently blown and beaten under 13 with pasteur pipet, cell can be complete in flakes
Portion comes off.
Embodiment 3
The present embodiment prepares a kind of temperature sensitive culture surface product, and detailed preparation process is as follows：
According to mol ratio 90:10 weigh NIPA and allyltriethoxysilane, then weighing mole respectively
Number is 0.8% N,N methylene bis acrylamide of the above two sums, benzoyl peroxide, Benzoinum, is eventually adding as total matter
As solvent, stirring makes it fully dissolve to the oxolane of amount fraction 40%, stand-by with 300 mesh sieve net filtrations.
Silicate glass platelet substrate material is soaked respectively in sodium hydrate aqueous solution and hydrogen peroxide aqueous sulfuric acid
Process, dry.The temperature sensing material above preparing mixed solution is added to the resin liquid pool of ejection-type photocuring 3D printer
In, input the print code got well and changed with computer 3D software design in advance（In this embodiment, the temperature sensitive thickness of design printing is
10um）.Previously processed glass flake base material is placed on the printing bed of 3D printer, opens 3D printer and start to beat
Print.After 3D printer printing terminates, the glass flake printing temperature sensitive layer is put at heating in 100 DEG C of heated constant temperature passage
Reason 20min makes its further graft curing.After having processed, put it into soaking and washing 48h in RO water, super under 50KHz frequency
Sound cleans 5 minutes, and the contact angle then drying the quick culture surface of thermometric and water at 25 DEG C is 72o, and send to gamma radiation sterilization
After carry out 3T3 cell culture test.Cell culture condition is：5% CO2, basal medium containing 10% hyclone, 37 DEG C
Place 20min under the conditions of 20 DEG C after culture 48h in incubator, gently blown and beaten under 11 with pasteur pipet, cell can be complete in flakes
Portion comes off.
Embodiment 4
The present embodiment prepares a kind of temperature sensitive culture surface product, and detailed preparation process is as follows：
According to mol ratio 90:10 weigh NIPA and allyltriethoxysilane, then weighing mole respectively
Number is 0.8% N,N methylene bis acrylamide of the above two sums, azodiisobutyronitrile and Benzoinum, is eventually adding as total
As solvent, stirring makes it fully dissolve to the oxolane of mass fraction 50%, stand-by with 300 mesh sieve net filtrations.
Silicate glass platelet substrate material is soaked respectively in sodium hydrate aqueous solution and hydrogen peroxide aqueous sulfuric acid
Process, dry.The temperature sensing material above preparing mixed solution is added to the resin liquid pool of ejection-type photocuring 3D printer
In, input the print code got well and changed with computer 3D software design in advance（In this embodiment, the temperature sensitive thickness of design printing is
10um）.Previously processed glass flake base material is placed on the printing bed of 3D printer, opens 3D printer and start to beat
Print.After 3D printer printing terminates, the glass flake printing temperature sensitive layer is put at heating in 100 DEG C of heated constant temperature passage
Reason 20min makes its further graft curing.After having processed, put it into soaking and washing 48h in RO water, super under 50KHz frequency
Sound cleans 5 minutes, and the contact angle then drying the quick culture surface of thermometric and water at 25 DEG C is 75o, and send to gamma radiation sterilization
After carry out 3T3 cell culture test.Cell culture condition is：5% CO2, basal medium containing 10% hyclone, 37 DEG C
Place 20min under the conditions of 20 DEG C after culture 48h in incubator, gently blown and beaten under 15 with pasteur pipet, cell can be complete in flakes
Portion comes off.
Embodiment 5
The present embodiment prepares a kind of temperature sensitive culture surface product, and detailed preparation process is as follows：
According to mol ratio 90:10 weigh NIPA and allyltriethoxysilane, then weighing mole respectively
Number is 0.8% N,N methylene bis acrylamide of the above two sums, azodiisobutyronitrile and Benzoinum, is eventually adding as total
As solvent, stirring makes it fully dissolve to the oxolane of mass fraction 50%, stand-by with 300 mesh sieve net filtrations.
Silicate glass platelet substrate material is soaked respectively in sodium hydrate aqueous solution and hydrogen peroxide aqueous sulfuric acid
Process, dry.The temperature sensing material above preparing mixed solution is added to the resin liquid pool of ejection-type photocuring 3D printer
In, input the print code got well and changed with computer 3D software design in advance（In this embodiment, the temperature sensitive thickness of design printing is
15um）.Previously processed glass flake base material is placed on the printing bed of 3D printer, opens 3D printer and start to beat
Print.After 3D printer printing terminates, the glass flake printing temperature sensitive layer is put at heating in 100 DEG C of heated constant temperature passage
Reason 20min makes its further graft curing.After having processed, put it into soaking and washing 48h in RO water, super under 50KHz frequency
Sound cleans 5 minutes, and the contact angle then drying the quick culture surface of thermometric and water at 25 DEG C is 65o, and send to gamma radiation sterilization
After carry out 3T3 cell culture test.Cell culture condition is：5% CO2, basal medium containing 10% hyclone, 37 DEG C
Place 20min under the conditions of 20 DEG C after culture 48h in incubator, gently blown and beaten under 10 with pasteur pipet, cell can be complete in flakes
Portion comes off.
Embodiment 6
The present embodiment prepares a kind of temperature sensitive culture surface product, and detailed preparation process is as follows：
According to mol ratio 90:10 weigh NIPA and allyltriethoxysilane, then weighing mole respectively
Number is 0.8% N,N methylene bis acrylamide of the above two sums, azodiisobutyronitrile and Benzoinum, is eventually adding as total
As solvent, stirring makes it fully dissolve to the oxolane of mass fraction 50%, stand-by with 300 mesh sieve net filtrations.
Silicate glass platelet substrate material is soaked respectively in sodium hydrate aqueous solution and hydrogen peroxide aqueous sulfuric acid
Process, dry.The temperature sensing material above preparing mixed solution is added to the resin liquid pool of ejection-type photocuring 3D printer
In, input the print code got well and changed with computer 3D software design in advance（In this embodiment, the temperature sensitive thickness of design printing is
15um）.Previously processed glass flake base material is placed on the printing bed of 3D printer, opens 3D printer and start to beat
Print.After 3D printer printing terminates, the glass flake printing temperature sensitive layer is put at heating in 100 DEG C of heated constant temperature passage
Reason 30min makes its further graft curing.After having processed, put it into soaking and washing 48h in RO water, super under 50KHz frequency
Sound cleans 5 minutes, and the contact angle then drying the quick culture surface of thermometric and water at 25 DEG C is 68o, and send to gamma radiation sterilization
After carry out 3T3 cell culture test.Cell culture condition is：5% CO2, basal medium containing 10% hyclone, 37 DEG C
Place 20min under the conditions of 20 DEG C after culture 48h in incubator, gently blown and beaten under 11 with pasteur pipet, cell can be complete in flakes
Portion comes off.
Embodiment 7
The present embodiment prepares a kind of temperature sensitive culture surface product, and detailed preparation process is as follows：
According to mol ratio 90:10 weigh NIPA and allyltriethoxysilane, then weighing mole respectively
Number is 0.8% N,N methylene bis acrylamide of the above two sums, azodiisobutyronitrile and Benzoinum, is eventually adding as total
As solvent, stirring makes it fully dissolve to the oxolane of mass fraction 50%, stand-by with 300 mesh sieve net filtrations.
Silicate glass platelet substrate material is soaked respectively in sodium hydrate aqueous solution and hydrogen peroxide aqueous sulfuric acid
Process, dry.The temperature sensing material above preparing mixed solution is added to the resin liquid pool of ejection-type photocuring 3D printer
In, input the print code got well and changed with computer 3D software design in advance（In this embodiment, the temperature sensitive thickness of design printing is
10um）.Previously processed glass flake base material is placed on the printing bed of 3D printer, opens 3D printer and start to beat
Print.After 3D printer printing terminates, the glass flake printing temperature sensitive layer is put at heating in 100 DEG C of heated constant temperature passage
Reason 30min makes its further graft curing.After having processed, put it into soaking and washing 48h in RO water, super under 50KHz frequency
Sound cleans 10 minutes, and the contact angle then drying the quick culture surface of thermometric and water at 25 DEG C is 74o, and send to gamma radiation sterilization
After carry out 3T3 cell culture test.Cell culture condition is：5% CO2, basal medium containing 10% hyclone, 37 DEG C
Place 20min under the conditions of 20 DEG C after culture 48h in incubator, gently blown and beaten under 13 with pasteur pipet, cell can be complete in flakes
Portion comes off.
Embodiment 8
The present embodiment prepares a kind of temperature sensitive culture surface product, and detailed preparation process is as follows：
According to mol ratio 90:10 weigh NIPA and pi-allyl -3- triethoxysilane, then weigh respectively and rub
Your number is 0.8% N,N methylene bis acrylamide of the above two sums, azodiisobutyronitrile, Benzoinum, is eventually adding as total
As solvent, stirring makes it fully dissolve to the oxolane of mass fraction 50%, stand-by with 300 mesh sieve net filtrations.
Plastic substrate is carried out surface plasma hydrophilic treated, introduces hydroxyl and carboxylic group in substrate surface, so
Afterwards the temperature sensing material above preparing mixed solution is added in the resin liquid pool of ejection-type photocuring 3D printer, input is pre-
The print code first got well and changed with computer 3D software design（In this embodiment, design prints temperature sensitive thickness is 10um）.Will be front
The plastic basis material that face was processed is placed on the printing bed of 3D printer, opens 3D printer and starts to print.Treat 3D printer
After printing terminates, the plastic tab printing temperature sensitive layer being put into heat treated 30min in 85 DEG C of heated constant temperature passage makes it enter
One step graft curing.After having processed, put it into soaking and washing 48h in RO water, ultrasonic cleaning 5 minutes under 50KHz frequency, so
The quick culture surface of post-drying thermometric and water are 70 in 25 DEG C of contact angleo, and send to gamma radiation sterilization after carry out 3T3 cell training
Support test.Cell culture condition is：5% CO2, basal medium containing 10% hyclone, cultivate 48h in incubator for 37 DEG C
Place 20min under the conditions of 20 DEG C afterwards, gently blown and beaten under 11 with pasteur pipet, cell can all come off in flakes.
Do not needing to add by implementing 1 cell that the temperature sensitive culture surface of preparation is cultivated under the conditions of embodiment 8 various combination
Plus under conditions of pancreatin digestion, the results of cell can be realized at low temperature well it was demonstrated that the present invention can very efficiently
Prepare in uniform thickness, the good temperature sensitive culture surface of temperature-responsive.
Although an embodiment of the present invention has been shown and described, it will be understood by those within the art that：Not
Multiple changes, modification, replacement and modification can be carried out to these embodiments in the case of the principle of the disengaging present invention and objective, this
The scope of invention is limited by claim and its equivalent, without creative work improvements introduced etc., all should comprise
Within protection scope of the present invention.
Claims (10)
1. a kind of photocuring 3D printing method of temperature sensitive culture surface material is it is characterised in that comprise the following steps：
S1）Prepare photocuring 3D printing temperature sensing material；
S2）Temperature sensitive printed material jet printing on substrate surface and is carried out by ultraviolet or laser spoke using photocuring 3D printer
According to graft curing reaction；
S3）Itself and the further graft curing of base material is made in band type heated at constant temperature passage；
S4）The temperature sensitive culture surface product utilization ultra-pure water of preparation is carried out, dries.
2. temperature sensitive culture surface material according to claim 1 photocuring 3D printing method it is characterised in that：Described base
Material surface refer to surface treated containing abundant hydroxyl or carboxyl and glass, the pottery of condensation reaction can be carried out with silane
Porcelain or frosting.
3. temperature sensitive culture surface material according to claim 1 photocuring 3D printing method it is characterised in that：Described light
Solidification 3D printer refers to Ployjet class ejection-type photocuring 3D printer, and the light source of radiation curing adopts ultraviolet or laser light
Source, the layer thickness control of temperature sensitive polymer film printing solidification between 0.01～0.05mm, vertical print speed controls 0.1～
0.3mm/min.
4. temperature sensitive culture surface material according to claim 1 photocuring 3D printing method it is characterised in that：In S4 step
In rapid, temperature sensitive culture surface product adopts soaking and washing and the combined type cleaning process being cleaned by ultrasonic or individually adopts ultrasonic clear
Wash.
5. temperature sensitive culture surface material according to claim 4 photocuring 3D printing method it is characterised in that：Soak clear
Time of washing is 8～72h.
6. temperature sensitive culture surface material according to claim 4 photocuring 3D printing method it is characterised in that：Ultrasonic clear
The concussion frequency washed is 20～150KHz, and the time is 5～60min.
7. temperature sensitive culture surface material according to claim 1 photocuring 3D printing method it is characterised in that：Described light
Solidification 3D printing temperature sensing material includes temperature sensitive monomer, grafted monomers, cross-linking agent, chemical initiator, photoinitiator and solvent, its
In, the molar ratio of temperature sensitive monomer and grafted monomers is 95:5～85:In the range of 15, cross-linking agent is temperature sensitive monomer and grafted monomers
The 0.5%～1% of total moles quantity, chemical initiator is the 0.5%～1% of temperature sensitive monomer and grafted monomers total moles quantity, photosensitive
Initiator is the 0.5%～2% of temperature sensitive monomer and grafted monomers total moles quantity.
8. temperature sensitive culture surface material according to claim 7 photocuring 3D printing method it is characterised in that：Described temperature
Quick monomer includes NIPA or N- isopropyl -2- Methacrylamide, or NIPA is different with N-
The blend of propyl group -2- Methacrylamide, or NIPA and N- isopropyl -2- Methacrylamide is oligomeric
Thing, or mixed with the oligomer of N- isopropyl -2- Methacrylamide by the NIPA that molecular weight is less than 2000
The blend of gained.
9. temperature sensitive culture surface material according to claim 8 photocuring 3D printing method it is characterised in that：Described connect
Branch monomer include the silane coupling agent containing unsaturated double-bond, allyltriethoxysilane, allyltrimethoxysilanis,
2- methacrylic triethoxysilane, 2- methacrylic trimethoxy silane, propyl acrylate -3- trimethoxy silane,
Propyl acrylate -3- triethoxysilane, propyl methacrylate -3- trimethoxy silane, propyl methacrylate -3- three second
The one of which of TMOS or any two kinds or two or more mixture.
10. temperature sensitive culture surface material according to claim 9 photocuring 3D printing method it is characterised in that：Described
Cross-linking agent includes diacrylate-BDO ester, Ethylene glycol dimethacrylate, N, N- methylene bisacrylamide acid imide,
Bismaleimide amine material, described cross-linking agent contains two or more unsaturated double-bonds；Described chemical initiator includes
Azodiisobutyronitrile, 2,2'-Azobis(2,4-dimethylvaleronitrile) or di-t-butyl peroxide, benzoyl peroxide, cumyl peroxide, persulfuric acid
Potassium, Ammonium persulfate.；Described photoinitiator is using can produce the peace of free radical or anion, cation by Direct Resolution under light illumination
Breath perfume and its derivant or acetophenones derivant or benzophenone and its derivant；Described solvent is the non-proton molten of polarity
Agent, including DMF, N,N-dimethylacetamide, N-Methyl pyrrolidone, oxolane, Ketohexamethylene wherein
One or more, described solvent is not low toxicity compounds with hydroxyl and carboxyl reaction.
Priority Applications (1)
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| WO2020156358A1 (en) * | 2019-01-30 | 2020-08-06 | 高雄医学大学 | Slurry for photocuring 3d printing, preparation method therefor, and method of use thereof |
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