CN106367326B - Device for continuously producing and extracting pentamethylene diamine by immobilized cells - Google Patents
Device for continuously producing and extracting pentamethylene diamine by immobilized cells Download PDFInfo
- Publication number
- CN106367326B CN106367326B CN201610609856.8A CN201610609856A CN106367326B CN 106367326 B CN106367326 B CN 106367326B CN 201610609856 A CN201610609856 A CN 201610609856A CN 106367326 B CN106367326 B CN 106367326B
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- Prior art keywords
- pentanediamine
- solenoid valve
- pipeline
- isocon
- column
- Prior art date
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- 210000001822 immobilized cell Anatomy 0.000 title claims abstract description 29
- VHRGRCVQAFMJIZ-UHFFFAOYSA-N cadaverine Chemical compound NCCCCCN VHRGRCVQAFMJIZ-UHFFFAOYSA-N 0.000 title abstract description 15
- KJOMYNHMBRNCNY-UHFFFAOYSA-N pentane-1,1-diamine Chemical compound CCCCC(N)N KJOMYNHMBRNCNY-UHFFFAOYSA-N 0.000 claims abstract description 96
- 238000000605 extraction Methods 0.000 claims abstract description 47
- 239000007788 liquid Substances 0.000 claims abstract description 32
- 238000006243 chemical reaction Methods 0.000 claims abstract description 30
- WPEXVRDUEAJUGY-UHFFFAOYSA-B hexacalcium;(2,3,4,5,6-pentaphosphonatooxycyclohexyl) phosphate Chemical compound [Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])(=O)OC1C(OP([O-])([O-])=O)C(OP([O-])([O-])=O)C(OP([O-])([O-])=O)C(OP([O-])([O-])=O)C1OP([O-])([O-])=O WPEXVRDUEAJUGY-UHFFFAOYSA-B 0.000 claims abstract description 30
- 239000003480 eluent Substances 0.000 claims abstract description 24
- 238000010924 continuous production Methods 0.000 claims abstract description 17
- 238000010828 elution Methods 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 9
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 35
- 239000011575 calcium Substances 0.000 claims description 35
- 229910052791 calcium Inorganic materials 0.000 claims description 35
- 238000001514 detection method Methods 0.000 claims description 23
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 10
- 239000004472 Lysine Substances 0.000 claims description 10
- 239000000284 extract Substances 0.000 claims description 10
- 230000001580 bacterial effect Effects 0.000 claims description 8
- 210000004027 cell Anatomy 0.000 claims description 7
- 150000004985 diamines Chemical class 0.000 claims description 3
- 238000010979 pH adjustment Methods 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- CNPURSDMOWDNOQ-UHFFFAOYSA-N 4-methoxy-7h-pyrrolo[2,3-d]pyrimidin-2-amine Chemical compound COC1=NC(N)=NC2=C1C=CN2 CNPURSDMOWDNOQ-UHFFFAOYSA-N 0.000 claims 1
- 230000005611 electricity Effects 0.000 claims 1
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 2
- 238000001179 sorption measurement Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 14
- 239000000463 material Substances 0.000 description 11
- 238000004519 manufacturing process Methods 0.000 description 10
- 238000000034 method Methods 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 5
- KKEYFWRCBNTPAC-UHFFFAOYSA-N Terephthalic acid Chemical compound OC(=O)C1=CC=C(C(O)=O)C=C1 KKEYFWRCBNTPAC-UHFFFAOYSA-N 0.000 description 4
- 150000001412 amines Chemical class 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 230000008676 import Effects 0.000 description 4
- 239000012266 salt solution Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- PNEYBMLMFCGWSK-UHFFFAOYSA-N Alumina Chemical compound [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 3
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
- 238000009434 installation Methods 0.000 description 3
- NGVDGCNFYWLIFO-UHFFFAOYSA-N pyridoxal 5'-phosphate Chemical compound CC1=NC=C(COP(O)(O)=O)C(C=O)=C1O NGVDGCNFYWLIFO-UHFFFAOYSA-N 0.000 description 3
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- KRHYYFGTRYWZRS-UHFFFAOYSA-N Fluorane Chemical compound F KRHYYFGTRYWZRS-UHFFFAOYSA-N 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000004372 Polyvinyl alcohol Substances 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- WNLRTRBMVRJNCN-UHFFFAOYSA-N adipic acid Chemical compound OC(=O)CCCCC(O)=O WNLRTRBMVRJNCN-UHFFFAOYSA-N 0.000 description 2
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- GHVNFZFCNZKVNT-UHFFFAOYSA-N decanoic acid Chemical compound CCCCCCCCCC(O)=O GHVNFZFCNZKVNT-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
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- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 2
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 2
- 230000003100 immobilizing effect Effects 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 description 2
- XNGIFLGASWRNHJ-UHFFFAOYSA-N phthalic acid Chemical compound OC(=O)C1=CC=CC=C1C(O)=O XNGIFLGASWRNHJ-UHFFFAOYSA-N 0.000 description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 229960001327 pyridoxal phosphate Drugs 0.000 description 2
- 230000000284 resting effect Effects 0.000 description 2
- 235000010413 sodium alginate Nutrition 0.000 description 2
- 239000000661 sodium alginate Substances 0.000 description 2
- 229940005550 sodium alginate Drugs 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- NQPDZGIKBAWPEJ-UHFFFAOYSA-N valeric acid Chemical compound CCCCC(O)=O NQPDZGIKBAWPEJ-UHFFFAOYSA-N 0.000 description 2
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- WLJVXDMOQOGPHL-PPJXEINESA-N 2-phenylacetic acid Chemical compound O[14C](=O)CC1=CC=CC=C1 WLJVXDMOQOGPHL-PPJXEINESA-N 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- KBIWNQVZKHSHTI-UHFFFAOYSA-N 4-n,4-n-dimethylbenzene-1,4-diamine;oxalic acid Chemical compound OC(=O)C(O)=O.CN(C)C1=CC=C(N)C=C1 KBIWNQVZKHSHTI-UHFFFAOYSA-N 0.000 description 1
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 241001312219 Amorphophallus konjac Species 0.000 description 1
- 235000001206 Amorphophallus rivieri Nutrition 0.000 description 1
- 239000005711 Benzoic acid Substances 0.000 description 1
- 239000005632 Capric acid (CAS 334-48-5) Substances 0.000 description 1
- 241000206575 Chondrus crispus Species 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 1
- 229920002752 Konjac Polymers 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Natural products OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 1
- 235000021314 Palmitic acid Nutrition 0.000 description 1
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 229920005830 Polyurethane Foam Polymers 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000001361 adipic acid Substances 0.000 description 1
- 235000011037 adipic acid Nutrition 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 235000010419 agar Nutrition 0.000 description 1
- 229940072056 alginate Drugs 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- OBETXYAYXDNJHR-UHFFFAOYSA-N alpha-ethylcaproic acid Natural products CCCCC(CC)C(O)=O OBETXYAYXDNJHR-UHFFFAOYSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 235000010233 benzoic acid Nutrition 0.000 description 1
- 230000000035 biogenic effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
- 235000010410 calcium alginate Nutrition 0.000 description 1
- 239000000648 calcium alginate Substances 0.000 description 1
- 229960002681 calcium alginate Drugs 0.000 description 1
- OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 239000000017 hydrogel Substances 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 239000000252 konjac Substances 0.000 description 1
- 235000010485 konjac Nutrition 0.000 description 1
- 150000003951 lactams Chemical class 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000000467 phytic acid Substances 0.000 description 1
- 235000002949 phytic acid Nutrition 0.000 description 1
- 229940068041 phytic acid Drugs 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- -1 polysaccharide compound Chemical class 0.000 description 1
- 239000011496 polyurethane foam Substances 0.000 description 1
- 229910021426 porous silicon Inorganic materials 0.000 description 1
- 235000007682 pyridoxal 5'-phosphate Nutrition 0.000 description 1
- 239000011589 pyridoxal 5'-phosphate Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 229940005605 valeric acid Drugs 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C209/00—Preparation of compounds containing amino groups bound to a carbon skeleton
- C07C209/82—Purification; Separation; Stabilisation; Use of additives
- C07C209/86—Separation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
The invention discloses a device for continuously producing and extracting pentanediamine by immobilized cells, which comprises a raw material conveying part, a pentanediamine conversion part, a PH adjusting part, a column adsorption and elution part and a centrifugal extraction part, wherein a raw material tank, a pentanediamine conversion integrated tank and a calcium phytate column are circularly connected through a pipeline, the PH adjusting liquid tank is connected on a pipeline between the raw material tank and the pentanediamine conversion integrated tank through a pipeline, an eluent storage tank is connected with the calcium phytate column through a pipeline, and a centrifugal extractor is connected on the calcium phytate column. Compared with the prior art, the device for continuously producing and extracting the pentamethylene diamine by the immobilized cells can better solve the problem of continuous production and extraction of the pentamethylene diamine, is suitable for large-scale production operation of the pentamethylene diamine, and has the characteristics of simplicity and convenience in operation, high equipment utilization rate, stable product quality, full utilization of raw materials, easiness in popularization and the like.
Description
Technical field
The present invention relates to a kind of production extraction equipment of compound, especially a kind of immobilized cell continuous production extracts penta
The device of diamines belongs to industrial processes production technical field.
Background technology
Pentanediamine (cadaverine, alias cadaverine) is the fuming liquids of colorless viscous, there is special odor.Fusing point：9
DEG C, it is dissolved in water, ethyl alcohol etc..Pentanediamine monomer is a kind of intermediate having important and extensive industrial use, is widely used in each
Kind polyamide product（Nylon）, chelating agent and additive etc..With the raising of environmental consciousness and the rise of biological economy, as flat
The pentanediamine of platform compound is increasingly paid attention to.The pentanediamine of biological method mass production can be substituted and traditional be given birth to by chemical industry
Biogenic amine-hexamethylene diamine of production, high-quality high molecular material-novel nylon can be synthesized by carrying out polymerisation with binary organic acid, be
A kind of environment-friendly type, sustainable development, heat safe biological plastics, have a wide range of applications.Traditional chemistry closes
, pollution environment harsh at law article part, is highly dependent on petrochemical industry.Resting cell production pentanediamine has economy, ring
The advantages of guarantor, sustainable development.The pentanediamine of traditional resting cell production pentanediamine high concentration is larger to cytotoxic, carefully
Born of the same parents cannot reuse, and separation is complex.
Invention content
The technical problem to be solved in the invention be in view of the above shortcomings of the prior art, and provide it is a kind of it is easy to operate,
The high pentanediamine of utilization rate of equipment and installations produces extraction equipment.
In order to solve the above technical problems, the technical solution adopted by the present invention is：
A kind of device of immobilized cell continuous production extraction pentanediamine, including feedstock transportation part, pentanediamine conversion portion
Divide, PH adjusts part, column absorb-elute part and Centrifugical extraction part；Feedstock transportation part includes head tank；Pentanediamine turns
It includes that pentanediamine converts integrated tank to change part, has immobilized cell bacterial spawn in the pentanediamine conversion integrated tank；PH adjustment portions
Divide includes that PH adjusts flow container；Column absorb-elute part includes eluent holding vessel and phytic acid calcium column；Centrifugical extraction part include from
Heart extracter；The head tank, pentanediamine conversion integrated tank and phytic acid calcium column are connected by circulation by pipeline, and the PH adjusts flow container
It is connected on the pipeline between head tank and pentanediamine conversion integrated tank by pipeline, the eluent holding vessel is connected by pipeline
Phytic acid calcium column is connect, the centrifugal extractor connects on phytic acid calcium column.
As further preferred embodiment, having heaters is set between the head tank and pentanediamine conversion integrated tank.
As further preferred embodiment, the feedstock transportation part further include the first delivery pump, the first solenoid valve and
Second solenoid valve；The head tank, the first delivery pump, the first solenoid valve and second solenoid valve are connected by circulation by pipeline, described
Heater is connected to by pipeline on the pipeline between the first solenoid valve and second solenoid valve.
As further preferred embodiment, it further includes the second delivery pump, third solenoid valve and that the PH, which adjusts part,
Four solenoid valves；The PH adjusts flow container, the second delivery pump, third solenoid valve and the 4th solenoid valve and is connected by circulation by pipeline, institute
It states on the pipeline that heater is connected to by pipeline between third solenoid valve and the 4th solenoid valve.
As further preferred embodiment, column absorb-elute part further includes third delivery pump, the 5th solenoid valve
With the 6th solenoid valve；The eluent holding vessel, third delivery pump, the 5th solenoid valve and the 6th solenoid valve are recycled by pipeline to be connected
It connects, the phytic acid calcium column is connected to by pipeline on the pipeline between the 5th solenoid valve and the 6th solenoid valve.
As further preferred embodiment, the phytic acid calcium column is divided into the first calcium column and the second calcium column, perforation the
The first isocon and the 4th isocon is arranged in one calcium column, and the second isocon and third isocon is arranged in the second calcium column of perforation；It is described
First calcium column and the first isocon on second calcium column the same end and the second isocon connect eluent holding vessel, the other end jointly
On the first isocon and the second isocon connect Centrifugical extraction part jointly；On first calcium column and second calcium column the same end
Four isocons and third isocon connect pentanediamine conversion integrated tank, the 4th isocon on the other end and third isocon jointly
Common connection head tank；It is respectively set on first isocon, the 4th isocon, the second isocon and third isocon useful
In the valve of control switch.
As further preferred embodiment, be additionally provided on the pentanediamine conversion integrated tank PH detection feedback systems,
Temperature detection reponse system, chuck and lysine concentration detection device.
As further preferred embodiment, the Centrifugical extraction part further includes the extractant being connected by circulation by pipeline
Holding vessel, the 4th delivery pump, the 7th solenoid valve and the 8th solenoid valve, the centrifugal extractor are connected to the 7th electromagnetism by pipeline
On pipeline between valve and the 8th solenoid valve.
As further preferred embodiment, the Centrifugical extraction part further includes that raffinate collecting tank and extract liquor are collected
Tank.
As further preferred embodiment, the Centrifugical extraction part is at least provided with three centrifugal extractors.
Advantageous effect
Compared with prior art, the device of a kind of immobilized cell continuous production extraction pentanediamine of the invention, can be compared with
Solve the problems, such as well pentanediamine continuous production extraction, bacterium cell is converted using material liquid, obtain concentration it is relatively low and
It is mixed in the pentanediamine of solution, then the higher pentanediamine of content is obtained by elution and Centrifugical extraction, the present apparatus is suitable for penta 2
The large-scale production of amine operates, the device have easy to operate, utilization rate of equipment and installations is high, product with stable quality, raw material make full use of,
The features such as easy to spread, specifically has the following advantages：
Raw material availability is high, production efficiency is high, more environmentally-friendly；
It is adjusted using on-line checking reponse system, it is easy to operate to adjust rapidly；
Utilization rate of equipment and installations is high, economic security；
Production process is closed, safety and sanitation；
Product with stable quality.
Description of the drawings
Fig. 1 is the structural schematic diagram of the present invention；
Fig. 2 is the flow chart of the present invention；
Fig. 3 is distribution map of the isocon on phytic acid calcium column；
Fig. 4 is the structural schematic diagram of pentanediamine conversion integrated tank；
Wherein, 11- head tanks, the first solenoid valves of 12-, 13- second solenoid valves, the first delivery pumps of 14-, 21- pentanediamines turn
Change integrated tank, 22-PH detection feedback systems, 23- temperature detection reponse systems, 24- chucks, 25- immobilized cell bacterial spawns, 26-
Lysine concentration detection device, 31-PH adjusting flow containers, 32- third solenoid valves, the 4th solenoid valves of 33-, the second delivery pumps of 34-,
41- eluent holding vessels, the 5th solenoid valves of 42-, the 6th solenoid valves of 43-, 44- third delivery pumps, 51- centrifugal extractors, 52- extractions
Take solvent holding vessel, the 7th solenoid valves of 53-, the 8th solenoid valves of 54-, the 4th delivery pumps of 55-, 56- raffinate collecting tanks, 57- extractions
Take liquid collecting tank, 61- heaters, 71- the first calcium columns, 72- the second calcium columns, the first isocons of 73-, the 4th isocons of 74-, 75-
Second isocon, 76- third isocons.
Specific implementation mode
The optimal technical scheme that the invention will now be described in detail with reference to the accompanying drawings.
As shown, a kind of device of immobilized cell continuous production extraction pentanediamine of the present invention, including feedstock transportation
Partly, pentanediamine transform portion, PH adjust part, column absorb-elute part and Centrifugical extraction part；
Production pentanediamine bacterial strain refers to patent《A kind of genetic engineering bacterium and its purposes in producing 1,5- pentanediamines》Application
Number CN2014107999948.8, method for immobilizing cell refer to《Patent it is a kind of tolerance toxic products method for immobilizing cell and
Its immobilized cell produces 1,5- pentanediamine techniques》Application number 201610368936.9.
Feedstock transportation part includes head tank 11, the material liquid in head tank 11 be lysine salt solution, PH be 5.0~
8.5, preferably 6.6~7.0；
Pentanediamine transform portion includes pentanediamine conversion integrated tank 21, is additionally provided on the pentanediamine conversion integrated tank 21
PH detection feedback systems 22, temperature detection reponse system 23, chuck 24 and lysine concentration detection device 26, pentanediamine conversion one
Immobilized cell bacterial spawn 25 in body tank 21, immobilized cell material therefor, which is polymer carrier, can enumerate such as fine jade
Fat, carragheen, alginate, cellulose, gelatin, collagen polyacrylamide, polyvinyl alcohol, polyurethane foam, light-cured resin
Deng；Inorganic carrier material can be enumerated such as kaolin, porous silicon, activated carbon, cellular glass, aluminium oxide；Composite carrier
Such as sodium alginate/SiO can be enumerated2Composite hydrogel, calcium alginate, polyethylene glycol, polyvinyl lactam compounding carrier, κ-card
Draw glue and konjac polysaccharide compound gel, gelatin, agar, sodium alginate polyvinyl alcohol composite carrier etc..Above-mentioned material can be used alone
Or it is used in combination two or more；The lysine concentration detection device 26 of pentanediamine conversion integrated tank 21 detects lysine content（g/l）
It is 0~60, preferably 00~30；
PH adjustment portions point include that PH adjusts flow container 31, and the feed liquid that PH adjusts batch can 31 is acid solution, molten as acidity
Liquid can enumerate such as hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, sulfurous acid, hydrofluoric acid, carbonic acid, hydrosulphuric acid, boric acid, formic acid, acetic acid, third
Acid, butyric acid, octanoic acid, adipic acid, ethanedioic acid, malonic acid, succinic acid, maleic acid, tartaric acid, benzoic acid, phenylacetic acid, O-phthalic
Acid, terephthalic acid (TPA), valeric acid, caproic acid, capric acid, stearic acid, palmitic acid, acrylic acid etc..Above-mentioned acid solution can be used alone or
It is used in combination two or more；
Feedstock transportation part and PH adjust part and are separately connected heater 61 by pipeline, and heater 61 is directly connected to two
Amine transform portion；
Column absorb-elute part includes eluent holding vessel 41 and phytic acid calcium column, in order to ensure good elution effect elution
Liquid holding vessel 41, the PH of eluent is 4.5~13.4 in eluent holding vessel 41；
Centrifugical extraction part includes centrifugal extractor 51, and the Centrifugical extraction part is at least provided with three centrifugal extractors
51；
The head tank 11, pentanediamine conversion integrated tank 21 and phytic acid calcium column are connected by circulation by pipeline, and the PH is adjusted
Flow container 31 is connected to by pipeline on the pipeline between head tank 11 and pentanediamine conversion integrated tank 21, the eluent holding vessel
41 connect phytic acid calcium column by pipeline, and the centrifugal extractor 51 connects on phytic acid calcium column；The head tank 11 turns with pentanediamine
Having heaters 61 is set between change integrated tank 21.
Further, the feedstock transportation part further includes the first solenoid valve 12, second solenoid valve 13 and the first delivery pump
14；It the head tank 11, the first delivery pump 14, the first solenoid valve 12 and second solenoid valve 13 and is connected by circulation by pipeline, institute
It states on the pipeline that heater 61 is connected to by pipeline between the first solenoid valve 12 and second solenoid valve 13, is formed into a loop, this is
Make head tank 11 can be by the first delivery pump 14, the first solenoid valve 12 and second solenoid valve 13 again after discharging excessive raw material
Head tank 11 is returned, avoids wasting.
Further, it further includes third solenoid valve 32, the 4th solenoid valve 33 and the second delivery pump 34 that the PH, which adjusts part,；
The PH adjusts flow container 31, the second delivery pump 34, third solenoid valve 32 and the 4th solenoid valve 33 and is connected by circulation by pipeline, described
Heater 61 is connected to by pipeline on the pipeline between third solenoid valve 32 and the 4th solenoid valve 33, and forming circuit, this is to make
PH adjusts flow container 31 can pass through the second delivery pump 34, third solenoid valve 32 and the 4th solenoid valve after discharging excessive adjusting liquid
33, which return again to PH, adjusts flow container 31, avoids wasting.
Further, column absorb-elute part further includes the 5th solenoid valve 42, the 6th solenoid valve 43 and third conveying
Pump 44；The eluent holding vessel 41, third delivery pump 44, the 5th solenoid valve 42 and the 6th solenoid valve 43 are recycled by pipeline to be connected
It connecing, the phytic acid calcium column is connected to by pipeline on the pipeline between the 5th solenoid valve 42 and the 6th solenoid valve 43, forming circuit,
This is that eluent holding vessel 41 is allow to pass through third delivery pump 44, the 5th solenoid valve 42 and the 6th in the excessive eluent of release
Solenoid valve 43 returns again to eluent holding vessel 41, avoids wasting.
Further, the phytic acid calcium column is divided into the first calcium column 71 and the second calcium column 72, the first calcium column 71 of perforation setting the
The second isocon 75 and third isocon 76 is arranged in one isocon 73 and the 4th isocon 74, the second calcium column 72 of perforation；Described
The first isocon 73 and the second isocon 75 on 72 the same end of one calcium column 71 and the second calcium column connect eluent holding vessel jointly
41, the first isocon 73 and the second isocon 75 on the other end connection Centrifugical extraction part jointly；First calcium column 71 and second
Connection pentanediamine converts integrated tank 21 jointly for the 4th isocon 74 and third isocon 76 on 72 the same end of calcium column, on the other end
The 4th isocon 74 and third isocon 76 jointly connection head tank 11；First isocon 73, the 4th isocon 74,
The valve of control switch is separately provided on two isocons 75 and third isocon 76.
Further, the Centrifugical extraction part further includes the extractant holding vessel the 52, the 7th being connected by circulation by pipeline
Solenoid valve 53, the 8th solenoid valve 54 and the 4th delivery pump 55, the centrifugal extractor 51 are connected to the 7th solenoid valve by pipeline
53 and the 8th pipeline between solenoid valve 54 on, forming circuit, this is that extractant holding vessel 52 is made to discharge excessive extraction
Solvent can return again to extractant holding vessel 52 by the 4th delivery pump 55, the 7th solenoid valve 53 and the 8th solenoid valve 54, keep away
Exempt to waste；The Centrifugical extraction part further includes raffinate collecting tank 56 and extract liquor collecting tank 57.
In use, material liquid lysine salt solution is discharged from head tank 11, integrated tank 21 is converted into pentanediamine, with penta 2
The contact of immobilized cell bacterial spawn 25 in amine conversion integrated tank 21 forms pentanediamine, passes through on pentanediamine conversion integrated tank 21
PH detection feedback systems 22 detect, when PH is too high or too low, PH adjust flow container 31 then start release adjust liquid to material liquid into
Row is adjusted, and temperature detection reponse system 23 is detected, and when temperature is less than 36 degree, heater 61 starts, to subsequently entering penta
Diamines converts the material liquid heat treatment of integrated tank 21, and chuck 24 can keep equalized temperature, and temperature is allowed to be in suitable model
It encloses, lysine concentration detection device 26 can detect lysine concentration in pentanediamine conversion integrated tank 21, contain pentanediamine raw material
Liquid discharges into phytic acid calcium column from pentanediamine conversion integrated tank 21, and the first calcium column 71 and the second calcium column 72 in turn carry out pentanediamine
Elution procedure and absorption process enter after the first calcium column 71 containing pentanediamine material liquid and carry out pentanediamine absorption, and the second calcium
Column 72 injects eluent by eluent holding vessel 41 and carries out pentanediamine elution, just by eluting after the first calcium column 71 adsorbs pentanediamine
Liquid holding vessel 41 injects eluent and carries out pentanediamine elution, and material liquid then enters the second calcium column 72 and carries out pentanediamine absorption, instead
Multiple cycle operation, the first isocon 73 are turned on and off with the 4th isocon 74 under the control of valve simultaneously, the second isocon
75 are turned on and off with third isocon 76 under the control of valve simultaneously, when the first isocon 73 and the 4th isocon 74 are opened
When, the second isocon 75 is in third isocon 76 closes, when the second isocon 75 is in third isocon 76 to be opened,
Then the first isocon 73 and the 4th isocon 74, which are in, closes, and the solution containing pentanediamine then enters centrifugal extractor after elution
51, material liquid is adsorbed on after the first calcium column 71 and the second calcium column 72 to be come back to the preparation of head tank 11 and recycles again again, pentanediamine
Solution carries out centrifugal treating in centrifugal extractor 51, and extractant holding vessel 52 injects extract liquor, centrifugation to centrifugal extractor 51
The pentanediamine extraction solution of high concentration enters extract liquor collecting tank 57 after processing, and raffinate then enters raffinate collecting tank 56.
Embodiment 1
The method of the immobilized cell of referenced patents and step immobilized cell are uniformly placed on pentanediamine conversion integrated tank
In 21 immobilized cell bacterial spawn 25.To ensure that immobilized cell has larger contact area, immobilized cell bacterial spawn 25 is by more
The uniform netted column box of stainless steel wire of layer assembles.The power that temperature detection reponse system 23 regulates and controls heater 61 ensures to enter
The lysine salt solution temperature that pentanediamine converts integrated tank 21 is 37 DEG C, and PH detection feedback systems 22 adjust 32 He of third solenoid valve
4th solenoid valve 33 ensures every time identical into the ph of the lysine salt solution of pentanediamine conversion integrated tank 21.In feed liquid in proportion
3：1000（mmol：ml）Phosphopyridoxal pyridoxal phosphate is added, by adjusting the first solenoid valve 12 and second solenoid valve 13, feed change tank 11
Flow, lysine concentration detection device 26 detects remaining lysine concentration in pentanediamine conversion integrated tank 21, now by material liquid
By centrifuging diluted processing vapor detection pentanediamine concentration, obtains data by calculation processing and be shown in Table one：
Table one
| Flow | Lysine salt concentration | Pentanediamine concentration | Every gram of cell produces amine amount |
| 200.0ml/h | 250.0g/l | 154.73g/l | 6.19g/g |
| 250.0ml/h | 250.0g/l | 106.23g/l | 4.2 g/g |
| 300.0ml/h | 250.0g/l | 92.13 g/l | 3.67g/g |
Embodiment 2
When solution containing pentanediamine flows through phytic acid calcium column, the pentanediamine in solution can be adsorbed on phytic acid calcium by phytic acid calcium column
On column, the concentration of pentanediamine in solution is reduced, while reducing the ph of solution.But the not sufficiently stable absorption of absorption in this, warp
It crosses experiment to find to be eluted the part pentanediamine that phytic acid calcium column adsorbs with solution, the phytic acid calcium column after elution is highly concentrated
Degree pentanediamine conversion fluid can adsorb pentanediamine again again when flowing through, and achieve the purpose that recycling.First use same concentrations identical
The pentanediamine conversion fluid of volume flows through phytic acid calcium column and adsorbs pentanediamine, then is being protected respectively with the PH solution for being 4.5,7.0,13.4
It demonstrate,proves identical flow velocity and goes down to elute pentanediamine on phytic acid calcium column.It is dense before and after flowing through phytic acid column with vapor detection solution respectively
The concentration of pentanediamine, calculation processing obtain data and are shown in Table two in degree, eluent：
Table two
| The PH of eluent | Phytic acid calcium column pentanediamine amount | The pentanediamine amount of eluent | Eluting rate |
| 4.5 | 93.18mg | 48.23mg | 51.76% |
| 7.0 | 88.23mg | 52.87mg | 59.92% |
| 13.4 | 90.15mg | 57.66mg | 63.96% |
Embodiment 3
Three extraction centrifuges are first opened in order, and the frequency of regulation motor is 45hz, waits extraction centrifuge to stablize normal
Afterwards, first heavy phase is pumped into from heavy phase import with the flow of 100ml/min, when there is liquid outflow in the outlet of heavy phase, at once with
The flow of 100ml/min is pumped into light phase from light phase import, after waiting heavy and lights two-phase outlet stabilization to go out 15~20min of liquid, starts to remember
Heavy phase, the volume of light phase are recorded, and collects heavy and light two-phase outlet liquid again and measures volume, is simultaneously closed off after running a period of time
The pump of heavy and light two-phase entrance exports the motor of the closing centrifugal extractor not when there is liquid outflow when heavy and light two-phase, finally puts
Fall seldom quantity of fluid in centrifugal extractor.Measure feed liquid（Heavy phase into）, extractant（Light phase into）, extract liquor（Light phase goes out）,
Raffinate（Heavy phase goes out）Volume, their pentanediamine contents of vapor detection.
Three extraction centrifuges are first opened in order, and the frequency of regulation motor is 45hz, waits extraction centrifuge to stablize normal
Afterwards, first heavy phase is pumped into from heavy phase import with the flow of 200ml/min, when there is liquid outflow in the outlet of heavy phase, at once with
The flow of 200ml/min is pumped into light phase from light phase import, after waiting heavy and lights two-phase outlet stabilization to go out 15~20min of liquid, starts to remember
Heavy phase, the volume of light phase are recorded, and collects heavy and light two-phase outlet liquid again and measures volume, is simultaneously closed off after running a period of time
The pump of heavy and light two-phase entrance exports the motor of the closing centrifugal extractor not when there is liquid outflow when heavy and light two-phase, finally puts
Fall seldom quantity of fluid in centrifugal extractor.Measure feed liquid（Heavy phase into）, extractant（Light phase into）, extract liquor（Light phase goes out）,
Raffinate（Heavy phase goes out）Volume, vapor detection pentanediamine content.
Twice three are shown in Table after data preparation
Table three
| Flow | Extract liquor pentanediamine content | Raffinate pentanediamine content | Extract raffinate volume ratio | Extraction yield |
| 100ml/min | 141.18 | 21.34 | 10:7 | 90.43% |
| 200ml/min | 166.34 | 69.73 | 7:10 | 62.64% |
The present invention can also be the series connection of multiple pentanediamine conversion integrated tanks, series connection and shared form in parallel.
Certainly, the present invention can also have other various embodiments, without deviating from the spirit and substance of the present invention, ripe
It knows those skilled in the art and makes various corresponding change and deformations, but these corresponding changes and change in accordance with the present invention
Shape should all belong to the protection domain of appended claims of the invention.
Claims (10)
1. a kind of device of immobilized cell continuous production extraction pentanediamine, it is characterised in that：Including feedstock transportation part, penta 2
Amine transform portion, PH adjust part, column absorb-elute part and Centrifugical extraction part；Feedstock transportation part includes head tank
（11）；Pentanediamine transform portion includes pentanediamine conversion integrated tank（21）, the pentanediamine conversion integrated tank（21）It is interior to have admittedly
Surely change cell bacterial spawn（25）；PH adjustment portions point include that PH adjusts flow container（31）；Column absorb-elute part includes eluent holding vessel
（41）With phytic acid calcium column；Centrifugical extraction part includes centrifugal extractor（51）；The head tank（11）, pentanediamine convert integrated tank
（21）It is connected by circulation by pipeline with phytic acid calcium column, the PH adjusts flow container（31）It is connected to head tank by pipeline（11）With penta
Diamines converts integrated tank（21）Between pipeline on, the eluent holding vessel（41）Phytic acid calcium column is connected by pipeline, it is described
Centrifugal extractor（51）It connects on phytic acid calcium column.
2. a kind of device of immobilized cell continuous production extraction pentanediamine according to claim 1, it is characterised in that：Institute
State head tank（11）Integrated tank is converted with pentanediamine（21）Between having heaters is set（61）.
3. a kind of device of immobilized cell continuous production extraction pentanediamine according to claim 2, it is characterised in that：Institute
It further includes the first delivery pump to state feedstock transportation part（14）, the first solenoid valve（12）And second solenoid valve（13）；The head tank
（11）, the first delivery pump（14）, the first solenoid valve（12）And second solenoid valve（13）It is connected by circulation by pipeline, the heater
（61）It is connected to the first solenoid valve by pipeline（12）With second solenoid valve（13）Between pipeline on.
4. a kind of device of immobilized cell continuous production extraction pentanediamine according to claim 2, it is characterised in that：Institute
It further includes the second delivery pump to state PH and adjust part（34）, third solenoid valve（32）With the 4th solenoid valve（33）；The PH adjusts liquid
Tank（31）, the second delivery pump（34）, third solenoid valve（32）With the 4th solenoid valve（33）It is connected by circulation by pipeline, the heating
Device（61）It is connected to third solenoid valve by pipeline（32）With the 4th solenoid valve（33）Between pipeline on.
5. a kind of device of immobilized cell continuous production extraction pentanediamine according to claim 2, it is characterised in that：Institute
It further includes third delivery pump to state column absorb-elute part（44）, the 5th solenoid valve（42）With the 6th solenoid valve（43）；The elution
Liquid holding vessel（41）, third delivery pump（44）, the 5th solenoid valve（42）It is connected by circulation by pipeline with the 6th solenoid valve, the plant
Sour calcium column is connected to the 5th solenoid valve by pipeline（42）With the 6th solenoid valve（43）Between pipeline on.
6. a kind of device of immobilized cell continuous production extraction pentanediamine according to claims 1 or 2 or 3 or 4 or 5,
It is characterized in that：The phytic acid calcium column is divided into the first calcium column（71）With the second calcium column（72）, penetrate through the first calcium column（71）Setting the
One isocon（73）With the 4th isocon（74）, penetrate through the second calcium column（72）Second isocon is set（75）With third isocon
（76）；The first calcium column（71）With the second calcium column（72）The first isocon on the same end（73）With the second isocon（75）Altogether
With connection eluent holding vessel（41）, the first isocon on the other end（73）With the second isocon（75）Common connection centrifugation extraction
Take part；First calcium column（71）With the second calcium column（72）The 4th isocon on the same end（74）With third isocon（76）Jointly
It connects pentanediamine and converts integrated tank（21）, the 4th isocon on the other end（74）With third isocon（76）Common connection raw material
Tank（11）；First isocon（73）, the 4th isocon（74）, the second isocon（75）With third isocon（76）Upper difference
It is provided with the valve for controlling switch.
7. a kind of device of immobilized cell continuous production extraction pentanediamine according to claim 6, it is characterised in that：Institute
State pentanediamine conversion integrated tank（21）On be additionally provided with PH detection feedback systems（22）, temperature detection reponse system（23）And chuck
（24）, lysine concentration detection device（26）.
8. a kind of device of immobilized cell continuous production extraction pentanediamine according to claim 1, it is characterised in that：Institute
It further includes the extractant holding vessel being connected by circulation by pipeline to state Centrifugical extraction part（52）, the 4th delivery pump（55）, the 7th electricity
Magnet valve（53）With the 8th solenoid valve（54）, the centrifugal extractor（51）It is connected to the 7th solenoid valve by pipeline（53）With the 8th
Solenoid valve（54）Between pipeline on.
9. a kind of device of immobilized cell continuous production extraction pentanediamine according to claim 8, it is characterised in that：Institute
It further includes raffinate collecting tank to state Centrifugical extraction part（56）With extract liquor collecting tank（57）.
10. a kind of device of immobilized cell continuous production extraction pentanediamine according to claim 1, it is characterised in that：
The Centrifugical extraction part is at least provided with three centrifugal extractors（51）.
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| CN106984061A (en) * | 2017-05-17 | 2017-07-28 | 南京工业大学 | Continuous countercurrent extraction process and equipment for 1, 5-pentanediamine |
| CN113015578B (en) * | 2018-09-18 | 2023-11-21 | 英威达纺织（英国）有限公司 | System and method for recovering amines and derivatives thereof from aqueous mixtures |
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| CN105368766A (en) * | 2015-05-20 | 2016-03-02 | 天津科技大学 | Genetically engineered bacterium for producing pentamethylene diamine and method for preparing pentamethylene diamine |
| CN105777555A (en) * | 2014-12-17 | 2016-07-20 | 上海凯赛生物技术研发中心有限公司 | Continuous purifying device of 1,5-pentanediamine, and method thereof |
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| ATE517190T1 (en) * | 2008-01-23 | 2011-08-15 | Basf Se | METHOD FOR THE FERMENTATIVE PRODUCTION OF 1,5-DIAMINOPENTANE |
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| CN105777555A (en) * | 2014-12-17 | 2016-07-20 | 上海凯赛生物技术研发中心有限公司 | Continuous purifying device of 1,5-pentanediamine, and method thereof |
| CN105368766A (en) * | 2015-05-20 | 2016-03-02 | 天津科技大学 | Genetically engineered bacterium for producing pentamethylene diamine and method for preparing pentamethylene diamine |
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