CN106362605A - Device used for preparing nucleotide monomer solution - Google Patents
Device used for preparing nucleotide monomer solution Download PDFInfo
- Publication number
- CN106362605A CN106362605A CN201610964108.1A CN201610964108A CN106362605A CN 106362605 A CN106362605 A CN 106362605A CN 201610964108 A CN201610964108 A CN 201610964108A CN 106362605 A CN106362605 A CN 106362605A
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- China
- Prior art keywords
- bottle
- pipeline
- solute
- nucleotide monomer
- anhydrous solvent
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F21/00—Dissolving
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/44—Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Saccharide Compounds (AREA)
Abstract
The invention discloses a device used for preparing a nucleotide monomer solution. The device comprises solute bottles, anhydrous solvent bottles and a bottom bracket. The four solute bottles correspond to the four anhydrous solvent bottles one to one, wherein each solute bottle and each anhydrous solvent bottle constitute a group, a first pipeline is arranged between the solute bottle and the anhydrous solvent bottle in each group, the four anhydrous solvent bottles are connected to four gas outlets in the bottom bracket through second pipelines respectively, gas inlets in the bottom bracket are connected to high pressure steel bottles loaded with inert gas through third pipelines, bottle mouths of the four solute bottles are provided with fourth pipelines, the other ends of the fourth pipelines are connected to fifth channels respectively, and the tail ends of gas outlets in the fifth pipelines are provided with one-way gas vent valves. The device used for preparing the nucleotide monomer solution solves the problem that solution water content cannot be effectively controlled in the process of manually preparing nucleotide monomers, and ensures the quality of a key raw material, namely the nucleotide monomer solution for compounding nucleic acid.
Description
Technical field
The present invention relates to nucleic acid synthesizer device field, specifically a kind of device for the preparation of nucleotide monomer solution.
Background technology
In global range, biotechnology and industry assume the situation accelerated development, as the important set of the fourth industrial revolution
Part, Main Developed Countries and emerging economy is become to make deployment to development biological industry one after another.Grind as biotechnology basis
Study carefully the nucleic acid (dna and rna) of material, its product quality and stability are most important for biotechnology research.
The nucleic acid synthesis methods of Global Access are phosphoramidite (phosphoramidite) solid-phase synthesis at present.The party
The solid support of method chemical modification is synthetic vectors, through carrier surface chemical functional group, coupling nucleosides acid monomers successively,
Again through ammonolysis purification, obtain single nucleic acid strands.In monomer coupling process, the moisture of reaction system and reaction reagent is to coupling efficiency
Impact is huge.Typically require control reagent moisture and be less than 10ppm.The preparation of in the past traditional nucleotide monomer solution relies primarily on
Manually, in the environment of low humidity relatively, the rapid acetonitrile solution preparing nucleotide monomer.This is to the proficiency level of personnel and environment
Require very high.
Content of the invention
The technical problem to be solved in the present invention is to provide a kind of device for the preparation of nucleotide monomer solution, and this device is got rid of
Abandon conventional manually-operated drawback, built the anhydrous environment of local malleation, substantially increase the preparation of nucleotide monomer solution
Stability, greatly reduces the uncontrollable factor that human intervention causes, and low to operating environment requirements.
In order to solve above-mentioned technical problem, the technical solution used in the present invention is:
A kind of device for the preparation of nucleotide monomer solution, including solute bottle, anhydrous solvent bottle, five logical, described solutes
Be stored with bottle and anhydrous solvent bottle nucleotide monomer and acetonitrile respectively, and described solute bottle and anhydrous solvent bottle are respectively equipped with four
And correspond, each solute bottle and each anhydrous solvent bottle are one group, between each group of solute bottle and anhydrous solution bottle
All it is provided with the first pipeline, the two ends of the first pipeline extend respectively into the bottom of bottle of solute bottle and anhydrous solution bottle, described four anhydrous
Solution bottle is passed through second pipe respectively and is connected with described five logical four central gas outlets, and described five logical central air inlets pass through
3rd pipeline connects the high-pressure cylinder equipped with noble gases, and described 3rd pipeline further respectively has switch valve and Stress control
Valve, the bottle mouth position of described four solute bottles is respectively equipped with the 4th pipeline, and the other end of the 4th pipeline respectively connected the 5th pipe
Road, the gas outlet end on described 5th pipeline is provided with one-way exhaust valve.
Further, each group of solute bottle and anhydrous solvent bottle are all completely cut off with outside air.
Further, described five logical, switch valve, pressure-control valves and one-way exhaust valve are medical grade equipment.
Preferably, described noble gases use high-pure anhydrous argon.
Preferably, described first pipeline, second pipe, the 3rd pipeline, the 4th pipeline, the 5th pipeline all use poly- four
Fluorothene material.
The invention has the beneficial effects as follows:
A kind of device for the preparation of nucleotide monomer solution that the present invention manufactures, this device has been abandoned and had manually been operated in the past
The drawbacks of, build the anhydrous environment of local malleation, substantially increase the stability of nucleotide monomer solution preparation, greatly reduce
The uncontrollable factor that human intervention causes, and low to operating environment requirements；Nucleotide monomer course of dissolution is placed in anhydrous malleation
Environment, the waterless operation of effective guarantee course of dissolution, substantially increase the quality of nucleotide monomer, effective control nucleotide monomer
The moisture of solution；By using high-pure anhydrous noble gases, being equipped with medical valve and politef pipeline, effective control dissolves
During introduced contaminants introducing.
Brief description
The present invention is further detailed explanation with reference to the accompanying drawings and detailed description.
Fig. 1 is front view of the present invention.
In figure: 1, solute bottle, 2, anhydrous solvent bottle, 3, five lead to, and 4, nucleotide monomer, 5, acetonitrile, the 6, first pipeline, 7, the
Two pipelines, the 8, the 3rd pipeline, 9, high-pressure cylinder, 10, switch valve, 11, pressure-control valve, the 12, the 4th pipeline, the 13, the 5th pipeline,
14th, one-way exhaust valve.
Specific embodiment
Below in conjunction with accompanying drawing, embodiments of the invention are described in detail, but the present invention can be defined by the claims
Implement with the multitude of different ways covering.
As shown in figure 1, the present invention provides a kind of device for the preparation of nucleotide monomer solution, including solute bottle 1, anhydrous
Solvent bottle 2, five leads to 3, and be stored with described solute bottle 1 and anhydrous solvent bottle 2 nucleotide monomer 4 and acetonitrile 5 respectively, described solute
Bottle 1 and anhydrous solvent bottle 2 are respectively equipped with four and correspond, and each solute bottle 1 and each anhydrous solvent bottle 2 are one
Group, is all provided with the first pipeline 6 between each group of solute bottle 1 and anhydrous solution bottle 2, the two ends of the first pipeline 6 extend respectively into
Solute bottle 1 and the bottom of bottle of anhydrous solution bottle 2, described four anhydrous solution bottles 2 pass through second pipe 7 respectively and work as with described five logical 3
In four gas outlets connect, the air inlets in the middle of described five logical 3 connect the high pressure steel equipped with noble gases by the 3rd pipeline 8
Bottle 9, described noble gases use high-pure anhydrous argon；Switch valve 10 and pressure is further respectively had on described 3rd pipeline 8
Control valve 11, setting pressure-control valve 14 is to control admission pressure, preventing from leading to because pressure is excessive fluid pressure big, no
Solution in aqueous solvent bottle 2 splashes；The bottle mouth position of described four solute bottles 1 is respectively equipped with the 4th pipeline 12, and the 4th pipeline 12
The other end respectively connected the 5th pipeline 13, the gas outlet end on described 5th pipeline 13 is provided with one-way exhaust valve 14, lead to
Cross one-way exhaust valve 14, while gas in solute bottle 1 can be made to exclude, completely cut off the air in the external world it is therefore prevented that in the air
Pollution and the entrance of moisture.
Each group of solute bottle 1 and anhydrous solvent bottle 2 are all completely cut off with outside air, prevent solute bottle 1 and anhydrous solvent bottle 2
By the pollution of in the air and entering of moisture, simultaneously it is therefore prevented that in anhydrous solvent bottle 2 noble gases leakage.
Described five logical 3, switch valve 10, pressure-control valve 11 and one-way exhaust valve 14 are medical grade equipment it is ensured that setting
Mechanical admixture and other pollutions is not introduced during received shipment row.
Described first pipeline 6, second pipe 7, the 3rd pipeline 8, the 4th pipeline 12, the 5th pipeline 13 all use poly- four
Fluorothene material, the distinctive chemical stability of politef material and physical stability, effective guarantee gas circuit fluid path long-term
Stable operation.
Working method: open switch valve 10, adjust pressure-control valve 11 to suitable pressure spot, noble gases pass through the 3rd
Pipeline 8 enters in five logical 3, and noble gases are discharged by five logical 3 four gas outlets, are entered respectively to four by second pipe 7
In anhydrous solvent bottle 2, by the enhancing of anhydrous solvent bottle 2 internal gas pressure, the acetonitrile 5 in anhydrous solvent bottle 2 is made to pass through the first pipeline 6
Have respectively entered in corresponding solute bottle 1, and the gas in solute bottle 1 pass through the 4th pipeline 12, the 5th pipeline 13 with
And be arranged on the one-way exhaust valve 14 of the 5th pipeline 13 gas outlet end and discharge, then, will be molten for nucleotide monomer 4 by acetonitrile 5
Solution, thus obtain nucleotide monomer solution.
Invention described above embodiment, does not constitute limiting the scope of the present invention, any in the present invention
Spirit and principle within modification, equivalent and improvement of being made etc., should be included in the claim protection model of the present invention
Within enclosing.
Claims (5)
1. a kind of for nucleotide monomer solution preparation device it is characterised in that include solute bottle, anhydrous solvent bottle, five lead to,
Be stored with described solute bottle and anhydrous solvent bottle nucleotide monomer and acetonitrile respectively, and described solute bottle and anhydrous solvent bottle are respectively
Be provided with four and correspond, each solute bottle and each anhydrous solvent bottle are one group, each group of solute bottle with no water-soluble
All it is provided with the first pipeline, the two ends of the first pipeline extend respectively into the bottom of bottle of solute bottle and anhydrous solution bottle, described between liquid bottle
Four anhydrous solution bottles are passed through second pipe respectively and are connected with described five logical four central gas outlets, described five logical central entering
QI KOU passes through the 3rd pipeline connection equipped with the high-pressure cylinder of noble gases, and described 3rd pipeline further respectively has switch valve and pressure
Force control valve, the bottle mouth position of described four solute bottles is respectively equipped with the 4th pipeline, and the other end of the 4th pipeline respectively connected
5th pipeline, the gas outlet end on described 5th pipeline is provided with one-way exhaust valve.
2. the device for the preparation of nucleotide monomer solution according to claim 1 is it is characterised in that each group of solute
Bottle and anhydrous solvent bottle are all completely cut off with outside air.
3. the device for the preparation of nucleotide monomer solution according to claim 1 is it is characterised in that described five lead to, open
Close valve, pressure-control valve and one-way exhaust valve and be medical grade equipment.
4. the device for the preparation of nucleotide monomer solution according to claim 1 is it is characterised in that described noble gases
Use high-pure anhydrous argon.
5. the device for the preparation of nucleotide monomer solution according to claim 1 is it is characterised in that described first pipe
Road, second pipe, the 3rd pipeline, the 4th pipeline, the 5th pipeline all use politef material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610964108.1A CN106362605A (en) | 2016-10-28 | 2016-10-28 | Device used for preparing nucleotide monomer solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610964108.1A CN106362605A (en) | 2016-10-28 | 2016-10-28 | Device used for preparing nucleotide monomer solution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106362605A true CN106362605A (en) | 2017-02-01 |
Family
ID=57893903
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610964108.1A Pending CN106362605A (en) | 2016-10-28 | 2016-10-28 | Device used for preparing nucleotide monomer solution |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106362605A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202527165U (en) * | 2012-02-27 | 2012-11-14 | 上海宝钢化工有限公司 | Combination device capable of avoiding volatilization and oxidization of solvents |
| CN105314576A (en) * | 2014-07-29 | 2016-02-10 | 盛美半导体设备（上海）有限公司 | Solvent supply system |
| CN205139157U (en) * | 2015-11-27 | 2016-04-06 | 阜阳欣奕华材料科技有限公司 | Quantitative transfer device of ultrapure solvent |
| CN206103730U (en) * | 2016-10-28 | 2017-04-19 | 通用生物系统（安徽）有限公司 | A device that is used for nucleotide monomer solution to prepare |
-
2016
- 2016-10-28 CN CN201610964108.1A patent/CN106362605A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202527165U (en) * | 2012-02-27 | 2012-11-14 | 上海宝钢化工有限公司 | Combination device capable of avoiding volatilization and oxidization of solvents |
| CN105314576A (en) * | 2014-07-29 | 2016-02-10 | 盛美半导体设备（上海）有限公司 | Solvent supply system |
| CN205139157U (en) * | 2015-11-27 | 2016-04-06 | 阜阳欣奕华材料科技有限公司 | Quantitative transfer device of ultrapure solvent |
| CN206103730U (en) * | 2016-10-28 | 2017-04-19 | 通用生物系统（安徽）有限公司 | A device that is used for nucleotide monomer solution to prepare |
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