CN106362194A - Dressing for repairing wound surface and preparing method thereof - Google Patents
Dressing for repairing wound surface and preparing method thereof Download PDFInfo
- Publication number
- CN106362194A CN106362194A CN201610935998.3A CN201610935998A CN106362194A CN 106362194 A CN106362194 A CN 106362194A CN 201610935998 A CN201610935998 A CN 201610935998A CN 106362194 A CN106362194 A CN 106362194A
- Authority
- CN
- China
- Prior art keywords
- keratin
- hair
- layer
- dressing
- sensitive adhesive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 21
- 239000010410 layer Substances 0.000 claims abstract description 90
- 102000011782 Keratins Human genes 0.000 claims abstract description 74
- 108010076876 Keratins Proteins 0.000 claims abstract description 74
- 210000004209 hair Anatomy 0.000 claims abstract description 62
- 238000001879 gelation Methods 0.000 claims abstract description 30
- 239000004820 Pressure-sensitive adhesive Substances 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 28
- 238000002360 preparation method Methods 0.000 claims description 25
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 18
- 239000000843 powder Substances 0.000 claims description 18
- 239000011324 bead Substances 0.000 claims description 16
- 239000000243 solution Substances 0.000 claims description 16
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 15
- 230000001954 sterilising effect Effects 0.000 claims description 14
- 239000002245 particle Substances 0.000 claims description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 239000008187 granular material Substances 0.000 claims description 8
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 7
- 238000000498 ball milling Methods 0.000 claims description 6
- 150000003839 salts Chemical class 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 150000003460 sulfonic acids Chemical class 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 5
- 230000008014 freezing Effects 0.000 claims description 5
- 238000007710 freezing Methods 0.000 claims description 5
- 238000011534 incubation Methods 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- 238000001556 precipitation Methods 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 238000004042 decolorization Methods 0.000 claims description 4
- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 claims description 4
- 229940048086 sodium pyrophosphate Drugs 0.000 claims description 4
- 235000019818 tetrasodium diphosphate Nutrition 0.000 claims description 4
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 claims description 4
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 3
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000011259 mixed solution Substances 0.000 claims description 3
- 238000000746 purification Methods 0.000 claims description 3
- 239000004744 fabric Substances 0.000 claims description 2
- 238000000227 grinding Methods 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims 1
- 102000002322 Egg Proteins Human genes 0.000 claims 1
- 108010000912 Egg Proteins Proteins 0.000 claims 1
- 229910017052 cobalt Inorganic materials 0.000 claims 1
- 239000010941 cobalt Substances 0.000 claims 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims 1
- 235000014103 egg white Nutrition 0.000 claims 1
- 210000000969 egg white Anatomy 0.000 claims 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims 1
- 229910052939 potassium sulfate Inorganic materials 0.000 claims 1
- 235000011151 potassium sulphates Nutrition 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 10
- 239000004745 nonwoven fabric Substances 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 3
- 208000035143 Bacterial infection Diseases 0.000 abstract 1
- 206010022998 Irritability Diseases 0.000 abstract 1
- 208000022362 bacterial infectious disease Diseases 0.000 abstract 1
- 230000002439 hemostatic effect Effects 0.000 abstract 1
- 230000035699 permeability Effects 0.000 abstract 1
- 231100000331 toxic Toxicity 0.000 abstract 1
- 230000002588 toxic effect Effects 0.000 abstract 1
- 206010052428 Wound Diseases 0.000 description 35
- 208000027418 Wounds and injury Diseases 0.000 description 35
- 230000029663 wound healing Effects 0.000 description 11
- 239000000463 material Substances 0.000 description 6
- 102000008186 Collagen Human genes 0.000 description 5
- 108010035532 Collagen Proteins 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 5
- 239000002671 adjuvant Substances 0.000 description 5
- 229920001436 collagen Polymers 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241000283898 Ovis Species 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 230000002500 effect on skin Effects 0.000 description 3
- 210000002950 fibroblast Anatomy 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000007654 immersion Methods 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 3
- 235000019394 potassium persulphate Nutrition 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 206010020751 Hypersensitivity Diseases 0.000 description 2
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 2
- 208000026935 allergic disease Diseases 0.000 description 2
- 230000007815 allergy Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000021164 cell adhesion Effects 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 229960000935 dehydrated alcohol Drugs 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 210000000416 exudates and transudate Anatomy 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 230000037308 hair color Effects 0.000 description 2
- 230000023597 hemostasis Effects 0.000 description 2
- 229940056582 human hair preparation Drugs 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- WEXRUCMBJFQVBZ-UHFFFAOYSA-N pentobarbital Chemical compound CCCC(C)C1(CC)C(=O)NC(=O)NC1=O WEXRUCMBJFQVBZ-UHFFFAOYSA-N 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 230000008591 skin barrier function Effects 0.000 description 2
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 206010063560 Excessive granulation tissue Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 229910002567 K2S2O8 Inorganic materials 0.000 description 1
- 206010057249 Phagocytosis Diseases 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 241000053227 Themus Species 0.000 description 1
- 206010053615 Thermal burn Diseases 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 229960004756 ethanol Drugs 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 210000001126 granulation tissue Anatomy 0.000 description 1
- 230000003370 grooming effect Effects 0.000 description 1
- 230000000025 haemostatic effect Effects 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 210000003701 histiocyte Anatomy 0.000 description 1
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 238000011532 immunohistochemical staining Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 229960001412 pentobarbital Drugs 0.000 description 1
- 230000008782 phagocytosis Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000008470 skin growth Effects 0.000 description 1
- 230000036560 skin regeneration Effects 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000008467 tissue growth Effects 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/00051—Accessories for dressings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/00987—Apparatus or processes for manufacturing non-adhesive dressings or bandages
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/01—Non-adhesive bandages or dressings
- A61F13/01021—Non-adhesive bandages or dressings characterised by the structure of the dressing
- A61F13/01029—Non-adhesive bandages or dressings characterised by the structure of the dressing made of multiple layers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/01—Non-adhesive bandages or dressings
- A61F13/01034—Non-adhesive bandages or dressings characterised by a property
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/01—Non-adhesive bandages or dressings
- A61F13/01034—Non-adhesive bandages or dressings characterised by a property
- A61F13/01042—Absorbency
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/02—Adhesive bandages or dressings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/64—Use of materials characterised by their function or physical properties specially adapted to be resorbable inside the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F2013/00089—Wound bandages
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Vascular Medicine (AREA)
- Epidemiology (AREA)
- Materials Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Manufacturing & Machinery (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention relates to a dressing for repairing a wound surface and a preparing method thereof. The dressing comprises a non-woven fabric layer, a keratin layer and a pressure-sensitive adhesive layer, the keratin layer and the pressure-sensitive adhesive layer are both arranged on the non-woven fabric layer, and the pressure-sensitive adhesive layer is arranged on the periphery of the keratin layer and fixedly connected with the keratin layer. Keratin is arranged in the keratin layer and is hair keratin, and the hair keratin is any one of granular hair keratin and gelation hair keratin. The dressing and the preparing method have the advantages that the hemostatic effect is good, and repairing of the wound surface can be effectively accelerated; bacterial infection can be effectively prevented; good biocompatibility is achieved; oozing of the wound surface is controlled and absorbed; air permeability is high; regenerated tissue is protected; toxic and side effects are avoided, and irritability is avoided; utilization is convenient.
Description
Technical field
The present invention relates to a kind of dressing is and in particular to a kind of dressing of wound repairing and preparation method thereof.
Background technology
In the prior art, relevant medical dressing is generally made up of non-woven fabrics, absorbent paper, cotton for wadding, leakproof film, this paper mold
Medical dressing liquid absorption is big, not modification after imbibition, does not produce fracture and reakdown phenomenon.If but this medical dressing be used for baby,
When scald, burn patient, dressing is easy to and wound adhesion, is not easily stripped, and is not absorbed by tissue.Therefore, this Shen
Ask someone research and establishment to be simulated the substrate of body tissue and function and mounting system as the emphasis of scientific research, and to rebuild or to recover
Skin barrier is the final goal of injured Wound treating.Biological dressing is the wound-surface cover being currently taken seriously, preferable wound surface
Dressing should effectively stop bacterium infection first, accelerate wound healing, should have good biocompatibility, control and absorption simultaneously
Wound exudate, suitable gas and vapor pass through, the characteristic such as protection cambium, acceleration wound healing.
Keratin biomaterials have penetrate into beneficial to cell, the three dimensional scaffold structure of adhesion and propagation, in cell adhesion and
Propagation aspect has the characteristic being substantially better than other materials, has neuroinduction simultaneously, also shows that very well in terms of hemostasis
Effect.Based on the good characteristic of keratin itself, the applicant is intended to set up a kind of Medical dressing, using this technology system
Standby keratin dressing, when using, can cover on wound area surface, keratin can promote as ordinary adjuvants
Wound healing, has moisture absorption simultaneously, keeps wound surface to be dried, promotes cell growth, the effect of wound healing.Avoid commonly auxiliary
Material sticks together the damage again of wound surface.Eliminate the misery of bleed during releasing many and patient, also will not leave behind chip and delay wound
Healing, for promote skin regeneration provide an environment being conducive to wound healing.
Content of the invention
The defect that the present invention is directed to prior art provides a kind of dressing of wound repairing and preparation method thereof.
The purpose of the present invention is to be achieved through the following technical solutions:
A kind of dressing of wound repairing, including nonwoven layer, keratin layer and pressure-sensitive adhesive layer, described keratin layer and pressure-sensitive
Glue-line may be contained within nonwoven layer, and described pressure-sensitive adhesive layer is arranged at the periphery of described keratin layer and is consolidated with described keratin layer
Fixed connection.
Further, in described keratin layer, keratic amount is 0.9～1.2g/m2, preferably 1g/m2.
Further, described dressing also includes off-style paper layer, and described off-style paper layer is completely covered described keratin layer and glues
Together on described pressure-sensitive adhesive layer.
Further, described off-style paper layer is made up of two parts, and two parts are overlapping at described keratin layer.
Further, hair-keratin is human hair's keratin or animal hair keratin, and animal hair includes Pilus Caprae seu Oviss etc..
Further, described keratin is hair-keratin, the granular hair-keratin of described hair-keratin bag and
Any one in the hair-keratin of gelation.
Further, the preparation method of described granular hair-keratin is as follows: s1: defat and softening: weigh hair
Carry out defat and softening；S2: decolouring: s3: cleaning, drying: the hair after decolouring is carried out, and is dried；S4: granule
Preparation: dried hair is shredded, and adds liquid nitrogen freezing to grind, obtain the hair coarse powder that length is 0.1～2mm；Will be thick
Powder is placed in ball grinder, puts into agate bead, adds normal saline, rotation, ball milling, till 50 μm～90 μm of particle diameter, then for
Hair-keratin granule is homogenized；S5: purification: add normal saline to be centrifuged in homogenate, abandoning supernatant, and remove precipitation
Top layer and bottom, take the hair-keratin of stage casing even particle size, plus normal saline mix continue centrifugation, repeat above-mentioned behaviour
Make 2～4 times, finally still take the hair-keratin of stage casing even particle size, then obtain highly purified granular hair angle
Albumen, standby after sterilizing；
Further, the defat in step s1 and softening process are: weigh hair, soaked 20～40 minutes with ethanol, leaching
During bubble at interval of stirring in 2～10 minutes once, soak and finish rear flowing water flushing；After flushing, hair is positioned over hypochlorous acid
Soak 20～40 minutes in the solution that sodium is prepared with water, in this immersion process, once, immersion finishes for interval stirring in 2～10 minutes
Rinsed with water afterwards, the hair after being softened；
Further, the decolorization in step s2 is: the hair after softening is positioned in de-inking solution and is decoloured,
The composition of described de-inking solution be sodium pyrophosphate 3～5 weight portion, potassium peroxydisulfate 1～2 weight portion, hydrogen peroxide 70～90 parts by volume,
Strong aqua ammonia 22～33 parts by volume and water 350～450 parts by volume；Described weight portion is g with the unit corresponding relation of parts by volume
And ml.
Further, the decolorization in step s2 is: the hair after softening in step s1 is positioned in de-inking solution
Decoloured, interval stirring in 10～30 minutes in decolorization once, is soaked and rinsed with water after finishing, washing de-inking solution off is
Only；The composition of this de-inking solution is sodium pyrophosphate 4 weight portion, potassium peroxydisulfate 1.5 weight portion, hydrogen peroxide 80 parts by volume, strong aqua ammonia
27.5 parts by volume and water 400 parts by volume；
Further, the cleaning in step s3, drying course are: the hair after decolouring is soaked in water, immersion finishes
Rinsed with water, filter, dry.
Further, the detailed process of step s4 is: dried hair is shredded, and adds liquid nitrogen freezing to grind, obtain
The hair coarse powder being 0.1～2mm to length；Hair coarse powder is placed in ball grinder, puts into agate bead, add normal saline, hair
The mass volume ratio sending out coarse powder with normal saline is 2:5, and ball milling 1～3 hour examines under a microscope size, until shape
Become about 60 μm -80 μm of particle diameter；The quantity of described agate bead is: the corresponding diameter 1cm agate bead of the hair coarse powder of every 1g 10 and straight
Footpath 6mm agate bead 50.
Further, the preparation method of the hair-keratin of described gelation is: takes prepared graininess in step s5
Hair-keratin, add water, fully mix, be positioned over incubation in incubator, to assuming more homogeneous color and character, obtain
To the hair-keratin of gelation, standby after sterilizing.
Further, the hair-keratin of described gelation can also be prepared by the following method: (1) hair-keratin becomes
Salt: weigh hair, add the mixed solution of second alcohol and water, heating, backflow, the Deca aqueous slkali in backflow, to ph value for 6.5～
Terminate when 7.5, then proceed to stir, make reaction completely, be filtrated to get into salt hair-keratin, be dried, be broken to micron order, freeze
Dry, that is, obtain hair-keratin sulfonic acid salt powder；(2) preparation of hair-keratin gel: weigh hair-keratin sulfonic acid salt fines
End, adds water, fully mixes, and is positioned over incubation in incubator, obtains hair-keratin gel.
Further, the hair-keratin of above-mentioned obtained described granular hair-keratin and described gelation is equal
Sterilization treatment to be carried out；Described sterilization treatment is to be sterilized using 60Coradiation.
Further, the concentration of the hair-keratin of described gelation is 0.5～5%, preferably 2～3%.
A kind of preparation method of the dressing of described wound repairing, methods described includes: s1: keratic preparation, angle egg
It is in vain hair-keratin, any in the hair-keratin of the granular hair-keratin of described hair-keratin bag and gelation
One kind, the preparation method of the hair-keratin of granular hair-keratin and gelation is with above-mentioned described method；S2: accurate
Standby nonwoven layer and pressure sensitive adhesive, the structure setting of adjuvant keratin and pressure sensitive adhesive being pressed wound repairing, in nonwoven layer, is repaired
The structure of the adjuvant of wound surface is ibid.
The invention provides a kind of dressing of wound repairing and preparation method thereof, it mainly has the beneficial effect that
1st, the dressing of the present invention adopts nonwoven layer and keratin layer, and with hair-keratin as raw material, non-woven fabrics are carrier,
Form the wound-surface cover with biological dressing property, play the partial action of skin barrier function；Meanwhile, there is good life
The thing compatibility, slowly can heal with wound and autologous skin growth, and hair-keratin can voluntarily dissolve and be absorbed by organisms, it is to avoid
Ordinary adjuvants stick together the damage again of wound surface, eliminate the misery of bleed during releasing many and patient, also will not leave behind chip and prolong
The healing of slow wound, the regeneration for promoting skin provides an environment being conducive to wound healing.
2nd, the dressing of the present invention adopts nonwoven layer, keratin layer and pressure-sensitive adhesive layer, and by pressure-sensitive adhesive layer around keratin
Layer is arranged on non-woven fabrics, is connected firmly between each layer；Importantly, using animal hair or human hair's keratin as former
Material, as carrier, then this dressing is without any side effects for non-woven fabrics, prepares dressing using hairs such as human hair or Pilus Caprae seu Oviss no immune
Originality, will not allergy；
3rd, each structure sheaf of the dressing of the present invention and using hair-keratin as the system of repair layer and hair-keratin
Standby process etc. is all interrelated, synergistic, and its collective effect makes dressing not only non-immunogenicity, will not allergy, also
Have following technical effect that (1) haemostatic effect is very good, and can effectively accelerate wound healing；(2) it effectively stops antibacterial sense
Dye, (3) have hygroscopicity, control and absorb wound exudate, keep wound surface to be dried, promote cell growth, wound healing
Effect；(4) breathability is strong, and suitable gas and vapor pass through, the advantage such as (5) protection cambium.
4th, the dressing of the present invention easy to use it is only necessary to tear off-style paper layer etc. and cover wound area surface just can,
User just can voluntarily use in the case of not having doctor etc. to instruct.
Brief description
Fig. 1 is the structural representation of the dressing in wound repairing described in the embodiment of the present invention；
Fig. 2 is the structural representation in light Microscopic observation hair-keratin described in the embodiment of the present invention；
Fig. 3 is in scanning electron microscope (1.69 × 10 described in the embodiment of the present invention3) under observe hair-keratin structure show
It is intended to；
Fig. 4 is in scanning electron microscope (1.25 × 10 described in the embodiment of the present invention3) under observe hair-keratin structure show
It is intended to；
Fig. 5 is in transmission electron microscope (1 × 10 described in the embodiment of the present invention5) under observe hair-keratin structural representation
Figure；
Fig. 6 is in transmission electron microscope (2 × 10 described in the embodiment of the present invention5) under observe hair-keratin structural representation
Figure；
Fig. 7 is the character of the hair-keratin of the gelation of 4% described in the embodiment of the present invention；
Fig. 8 is the dyeing display type collagen secretion situation of the Mus dermal fibroblast described in the embodiment of the present invention.
Wherein, 1, nonwoven layer；2nd, keratin layer；3rd, keratin layer；4th, off-style paper layer.
Specific embodiment
Purpose, technical scheme and advantage for making the embodiment of the present invention are clearer, specifically real below in conjunction with the present invention
Apply example technical scheme to be clearly and completely described it is clear that described embodiment is that a present invention part is real
Apply example, rather than whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art are not making creation
Property work under the premise of the every other embodiment that obtained, broadly fall into the scope of protection of the invention.
Embodiment 1
As shown in figure 1, a kind of dressing of wound repairing, including nonwoven layer 1, keratin layer 2, pressure-sensitive adhesive layer 3 and release
Ply of paper 4, keratin layer 2 and pressure-sensitive adhesive layer 3 may be contained within nonwoven layer 1, and pressure-sensitive adhesive layer 3 sets around the periphery of keratin layer 2
Put and be fixedly connected with keratin layer 2, pressure-sensitive adhesive layer 3 can make keratin layer 2 more firm with the connection of nonwoven layer 1.
Off-style paper layer 4 covers all keratin layer 2 and is bonded on pressure-sensitive adhesive layer 3, and this off-style paper layer 4 is by two parts structure
Become, and two near against each other sides of two parts overlap in the middle of dressing, that is, the midline in keratin layer 2 overlaps on one
Rise, then using more convenient it is only necessary to just may be used tearing two sides of the release paper at overlapping place.
Hair-keratin is contained, the content of hair-keratin is 1g/m in keratin layer 22.Hair-keratin is graininess
Hair-keratin.The preparation method of granular hair-keratin is as follows:
S1: defat: weigh 5g human hair, be placed in 1000ml beaker.Add 99.7% soaked in absolute ethyl alcohol 30 minutes,
Stirred once with Glass rod every 5 minutes, flowing water rinses；
S2: soften: by cleaning after hair be positioned over >=10% sodium hypochlorite 10ml and distilled water 90ml configuration solution
In, soak at room temperature 30 minutes, interval is stirred once for 5 minutes with Glass rod, and flowing water rinses；
S3: decolouring: by the weight of 5g hair, weigh 99% sodium pyrophosphate (na4p2o7) 4g (mass-volume concentration),
99.5% potassium peroxydisulfate (k2s2o8) 1.5g (mass-volume concentration), 30% hydrogen peroxide (h2o2) 80ml (volumetric concentration), 25% is dense
Ammonia 27.5ml (volumetric concentration), distilled water 400ml, are configured to de-inking solution, and the hair of softening is placed in this solution, place
In 37 DEG C of thermostatic water bath, stirred 1 time with Glass rod every 20 minutes, observe hair color to light yellow, this process used time one
As 2-3 hour, short more a lot of than traditional method；Hair after decolouring is placed on screen cloth, flowing water rinses, until washing decolouring off
Solution.
S4: cleaning, dry: remove the hair more than 12 hours after ionized water soaks above-mentioned decolouring, remove remaining reagent；Go
Ionized water rinses, and is placed in air dry oven 37 DEG C and dries overnight after filtration；
S5: grind: be placed in ceramic mortar after the Hair grooming shears after drying are shredded, add liquid nitrogen freezing to grind, extremely
Hair becomes 1mm length, obtains hair coarse powder；
S6: ball milling: weigh hair coarse powder 2g, be placed in ball grinder, put into agate bead, add normal saline 10ml, 460
Turn/min, ball milling 2 hours, be inverted the size of observed under electron microscope particle diameter, add normal saline 5ml and continue grinding 1.5
Individual hour, until form human hair's keratin granule homogenate of about 70 μm of particle diameter；The quantity of above-mentioned agate bead: diameter 1cm agate
20, Nao pearl, diameter 6mm agate bead 100；
S7: tubulature: in above-mentioned homogenate add normal saline 30ml, by the liquid rinse in ball grinder be transferred to 50ml from
In heart pipe；
S8: purification: centrifuge tube is placed in the Centrifuge Cup of centrifuge, 3000 turns/min of centrifugal speed, is centrifuged 20min, abandons
Remove supernatant, remove the top layer of keratin precipitation and bottom with key, take the keratin of stage casing even particle size move to new from
In heart pipe, plus normal saline 40ml mixes, and continues centrifugation once, abandoning supernatant, then removes the table of keratin precipitation with key
Layer and bottom, take the keratin of stage casing even particle size to move in new centrifuge tube, then plus normal saline 40ml mix, again from
The heart, abandons supernatant, then the top layer with key removal keratin precipitation and bottom, takes the keratin of stage casing even particle size to move to
In new centrifuge tube, obtain highly purified granular hair-keratin, because particle diameter is little, hence it appear that being keratin mud；Temporarily
When be placed on 4 DEG C and save backup；
S9: sterilizing: the highly purified granular hair-keratin obtaining is loaded in plastic bag, good seal, uses carton
Deliver to radiation center after packaging and carry out co60 radiation sterilization, dosage 12kg, be placed on 4 DEG C of refrigerators, stand-by；
The highly purified granular hair-keratin preparing is observed under om observation (× 20), it maintains hair
The fascicular texture that to send out original stable, as shown in Figure 2.
Fig. 3～6 are this granular hair-keratin electron microscope, and Fig. 3 behaves and acts in an unreasonable way section sem (1.69 × 103), Fig. 4
Face sem (1.25 × 103) is delivered in behaviour, and Fig. 5 is Crinis Carbonisatus tem (1 × 105), and Fig. 6 is Crinis Carbonisatus cortex tem (2 × 105).
From scanning electron microscope (sem), the Hair-material surface smoother processing through this patent method, no normal hair color
Scale structure, the convex patch shape in scrobicula；Transverse section hair cortex is still in no structure homogenizing shape.By the visible hair of transmission electron microscope (tem)
Send out melanin all disintegrates in keratin granular materialss.
Embodiment 2
In keratin layer in embodiment 1, the content of hair-keratin is 1.1g/m2, this hair-keratin is the hair of gelation
Send out keratin, the preparation method of the hair-keratin of gelation is as follows:
Take the water adding 5ml in the highly purified granular hair-keratin 200mg obtained by the embodiment of the present invention 1
In, fully mix, be positioned in incubator and be incubated at 37 DEG C, to assuming more homogeneous color and character；Sterilizing, obtains
The hair-keratin of gelation, its concentration is 4%.Fig. 7 is the character of 4% hair-keratin of gelation for concentration.
It should be noted that the granular hair-keratin that the present embodiment is used can also be made in embodiment 2
Standby.
Embodiment 3
In keratin layer in embodiment 1, the content of hair-keratin is 1.2g/m2, this hair-keratin is the hair of gelation
Send out keratin, the preparation method of the hair-keratin of gelation is as follows:
Take the water adding 5ml in the highly purified granular hair-keratin 125mg obtained by the embodiment of the present invention 2
In, fully mix, be positioned over incubation in incubator, to assuming more homogeneous color and character；Sterilizing, obtains gelation
Hair-keratin, its concentration is 2.5%, and its character is diluter than the character of the hair-keratin of the gelation in Fig. 7.
It should be noted that the granular hair-keratin that the present embodiment is used can also be made in embodiment 1
Standby.
Embodiment 4
In keratin layer in embodiment 1, the content of hair-keratin is 0.9g/m2, this hair-keratin is the hair of gelation
Send out keratin；The preparation method of the hair-keratin of gelation is as follows:
S1: weigh 10g human hair, put into three mouthfuls of burnings of the mixed solution equipped with 180ml dehydrated alcohol and 20ml distilled water
In bottle, heating was warming up to 75 DEG C after 15 minutes, and solution starts to flow back；Then, in backflow Deca 2ml naoh solution, to oxygen
Changing keratic ph value is 7, and backflow is terminated with Deca simultaneously, and the time continues 90 minutes；After Deca and backflow terminate, continue at 25 DEG C
Continuous stirring 5 hours, makes reaction completely, obtains into salt hair-keratin.
S2: salt hair-keratin will be become to leach, and rinsed with washing liquid, this washing liquid is that dehydrated alcohol is mixed with equivalent distilled water
Close, remove unreacted naoh, with ph detection paper whether in alkalescence, wash to not being in alkalescence, put into 60 DEG C of air dry ovens
Middle drying 6 hours, obtains the hair-keratin sulfonate of drying；
S3: through hair-keratin sulfonate obtained from above-mentioned process in fragility, in Meteor-style ball grinder, put into agate
Nao pearl, grinds under conditions of 460 turns/min, till pulverizing as 50 μm about of hair-keratin sulfonic acid salt powder, lyophilizing；
The quantity of agate bead can be: diameter 1cm agate bead 80, diameter 6mm agate bead 300；
The hair-keratin of s4: gelation: operate in super-clean bench, take the hair after the lyophilizing obtaining in 100mg step s3
Send out and in keratin sulfonic acid salt fines, add 5ml sterilized water, first adopt agitator to mix material by hand, more fully mixed with mediation mixing machine
Even, it is positioned over 37 DEG C of incubations about 3 hours, then obtain color and the more homogeneous hair-keratin gel of character, then, adopt
With the sterilizing of 60Coradiation method, obtain the hair-keratin of gelation, its concentration is 2%.
It should be noted that the human hair being used the present embodiment is changed to the animal hairs such as Pilus Caprae seu Oviss.
Embodiment 5
A kind of preparation method of the dressing of described wound repairing, methods described includes:
S1: keratic preparation, keratin is hair-keratin, the granular hair-keratin of described hair-keratin bag
With any one in the hair-keratin of gelation, the preparation method of granular hair-keratin is with the side in embodiment 1
Method, is not repeated herein explanation, the preparation method of the hair-keratin of gelation with the method in embodiment 2,3 or 4, here
Explanation is not repeated；
S2: prepare nonwoven layer and pressure sensitive adhesive, keratin and pressure sensitive adhesive are pressed described wound repairing in embodiment 1
In nonwoven layer, the content of hair-keratin, referring to embodiment 1,2,3 or 4, is also not repeated the structure setting of adjuvant here
Limit；
S3: prepare release paper, and release paper is covered all keratin layer 2 and is bonded on pressure-sensitive adhesive layer 3, this is release
Paper is made up of two parts, and two near against each other sides of two parts overlap in the middle of dressing, that is, in keratin layer 2
Overlap together at line, then using more convenient it is only necessary to just may be used tearing two sides of the release paper at overlapping place.
Embodiment 6
Take healthy sd rat, anaesthetized with pentobarbital (30mg/kg), open 1cm by its back spinal column side, scraper holostrome is cut
Except skin, form diameter 1.8cm, an area 2.54cm2Circular full thickness skin excision wound surface, offside antimere skin makees
For normal own control.Dressing paste prepared by the present invention, together in wound surface, makes the keratin layer of dressing and wound surface fit tightly.
Observe: after test group of animals wound, 3d days wound surface have reduced it is seen that new granulation tissue generates.21～28d after wound,
Most wound healings.It should be noted that he staining tissue slides are observed: survey skin corium and thicken, when 4 weeks average thickness is about
0.58mm, it is seen that assembling under substantial amounts of keratin granule corium, has cell and fibrous tissue growth, has many on a small quantity between keratin
Core giant cell, interior phagocytosis has keratin granule.
Immunohistochemical staining is observed: in section, Mus dermal fibroblast secretes collagen type in a large number, and collagen is fine
Dimension arrangement is tight, brown stain, and collagen type is interspersed with histiocyte；Filling position skin immunization group after 8th week
Weave chemistry dyes observation figure as shown in figure 8, Fig. 8 is the dyeing display type collagen secretion situation of Mus dermal fibroblast,
It follows that the recovery of Mus is very good.
Bacterium infection is observed: has no obvious bacterium infection situation injured to after fully recover.
Conclusion: the combine dressing effect highly significant of the present invention, in the case of there is no any medication, do not occur substantially sending out
21～28d after inflammation, and wound, most wound healings, hour angle protein gel can be degraded substantially within 12 weeks.
Specifically, the dressing of wound repairing of the present invention is conducive to the three dimensional scaffold structure that cell penetrates into, adheres to and breed,
There is in terms of cell adhesion and propagation the characteristic being substantially better than other materials, there is neuroinduction, simultaneously in terms of hemostasis
Also show that good effect；Coordinate the hair-keratin of the present invention again so as to effect is extremely notable.
Note: the studies above is all carried out in the case of Ethics Committee and experimenter's informed consent.
When being embodied as, in the present invention, the amount of hair-keratin can be more than 1.2g/m2, but amount is also impossible to all too much
Absorb, also can waste hair-keratin, so the amount of optimum is 0.9～1.2g/m2.
Finally it is noted that above-described each embodiment is merely to illustrate technical scheme, rather than to it
Limit；Although being described in detail to the present invention with reference to the foregoing embodiments, it will be understood by those within the art that:
It still can be modified to the technical scheme described in previous embodiment, or wherein part or all of technical characteristic is entered
Row equivalent；And these modifications or replacement, do not make the essence of appropriate technical solution depart from various embodiments of the present invention technical side
The scope of case.
Claims (10)
1. a kind of dressing of wound repairing it is characterised in that: include nonwoven layer, keratin layer and pressure-sensitive adhesive layer, described angle egg
White and pressure-sensitive adhesive layer may be contained within nonwoven layer, described pressure-sensitive adhesive layer be arranged at described keratin layer periphery and with described
Keratin layer is fixedly connected.
2. wound repairing according to claim 1 dressing it is characterised in that: described dressing also includes off-style paper layer, institute
State off-style paper layer described keratin layer to be completely covered and is bonded on described pressure-sensitive adhesive layer.
3. wound repairing according to claim 2 dressing it is characterised in that: described off-style paper layer is made up of two parts,
And two parts are overlapping at described keratin layer；Contain keratin in described keratin layer, described keratic content be 0.9～
1.2g/m2.
4. wound repairing according to claim 3 dressing it is characterised in that: described keratin be hair-keratin, institute
State any one that hair-keratin is in granular hair-keratin and the hair-keratin of gelation；
The preparation method of described granular hair-keratin is: s1: defat and softening: weighs hair and carries out defat and softening；
S2: decolouring: s3: cleaning, drying: the hair after decolouring is carried out, and is dried；The preparation of s4: granule: will be dried
Hair shreds, and adds liquid nitrogen freezing to grind, and obtains hair coarse powder；Coarse powder is placed in ball grinder, puts into agate bead, add life
Reason saline, rotation, ball milling, till particle diameter reaches 50 μm～90 μm, then it is homogenized for hair-keratin granule；S5: purification:
Normal saline is added to be centrifuged in homogenate, abandoning supernatant, and remove top layer and the bottom of precipitation, take stage casing granular size equal
Even hair-keratin, plus normal saline mixing continuation centrifugation, repeat aforesaid operations 2～4 times, finally still take stage casing granule big
Little uniform hair-keratin, then obtain highly purified granular hair-keratin, standby after sterilizing；
The preparation method of the hair-keratin of described gelation is: take prepared granular hair-keratin in step s5,
Add water, fully mix, be positioned over incubation in incubator, to assuming more homogeneous color and character, obtain the hair of gelation
Send out keratin, standby after sterilizing.
5. wound repairing according to claim 4 dressing it is characterised in that: the decolorization in step s2 is: will be soft
Hair after change is positioned in de-inking solution and is decoloured, and the composition of described de-inking solution is sodium pyrophosphate 3～5 weight portion, mistake
Potassium sulfate 1～2 weight portion, hydrogen peroxide 70～90 parts by volume, strong aqua ammonia 22～33 parts by volume and water 350～450 parts by volume.
6. wound repairing according to claim 4 dressing it is characterised in that: prepared granular hair-keratin
It is intended to carry out sterilization treatment with the hair-keratin of gelation；Described sterilization treatment is to adopt cobalt60Irradiation is sterilized.
7. wound repairing according to claim 4 dressing it is characterised in that: the detailed process of step s4 is: by drying
Hair afterwards shreds, and adds liquid nitrogen freezing to grind, and obtains the hair coarse powder that length is 0.1～2mm；Hair coarse powder is placed on ball
In grinding jar, put into agate bead, add normal saline, hair coarse powder is 2:5 with the mass volume ratio of normal saline, and ball milling 1～3 is little
When, examine under a microscope size, until forming about 60 μm -80 μm of particle diameter；The quantity of described agate bead is: the hair of every 1g
Send out the corresponding diameter 1cm agate bead of coarse powder 10 and diameter 6mm agate bead 50.
8. wound repairing according to claim 4 dressing it is characterised in that: the hair-keratin of described gelation dense
Spend for 0.5～5%.
9. the wound repairing according to any one of claim 4～8 dressing it is characterised in that: the hair of described gelation
Send out keratin can also be prepared by the following method:
(1) hair-keratin becomes salt: weighs hair, adds the mixed solution of second alcohol and water, heating, backflow, the Deca in backflow
Aqueous slkali, to ph value for 6.5～7.5 when terminate, then proceed to stir, make reaction completely, be filtrated to get into salt hair-keratin,
Dry, pulverize to micron order, lyophilizing, that is, obtain hair-keratin sulfonic acid salt powder；
(2) preparation of hair-keratin gel: weigh hair-keratin sulfonic acid salt powder, add water, fully mix, be positioned over temperature
It is incubated in case, sterilizing, obtain hair-keratin gel.
10. the dressing of wound repairing described in a kind of item claim according to any of the above preparation method it is characterised in that: institute
The method of stating includes: s1: keratic preparation；S2: prepare nonwoven layer and pressure sensitive adhesive, keratin and pressure sensitive adhesive be may be contained within no
Spin layer of cloth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610935998.3A CN106362194A (en) | 2016-11-01 | 2016-11-01 | Dressing for repairing wound surface and preparing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610935998.3A CN106362194A (en) | 2016-11-01 | 2016-11-01 | Dressing for repairing wound surface and preparing method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106362194A true CN106362194A (en) | 2017-02-01 |
Family
ID=57896628
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610935998.3A Pending CN106362194A (en) | 2016-11-01 | 2016-11-01 | Dressing for repairing wound surface and preparing method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106362194A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107126305A (en) * | 2017-06-12 | 2017-09-05 | 大连医科大学附属第医院 | A kind of maggot debridement bag |
| CN108714243A (en) * | 2017-06-08 | 2018-10-30 | 重庆大学 | Implant and preparation method thereof for post operation of hypertensive cerebral hemorrhage hemostasis |
| CN111202868A (en) * | 2020-01-17 | 2020-05-29 | 重庆大学 | Composition for preparing keratin gel dressing, preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101530636A (en) * | 2008-03-12 | 2009-09-16 | 章庆国 | Preparation method of injectable human hair keratin soft tissue filling material |
| US8273702B2 (en) * | 2006-02-17 | 2012-09-25 | Wake Forest University Health Sciences | Wound healing compositions containing keratin biomaterials |
| CN202477971U (en) * | 2011-12-27 | 2012-10-10 | 四川三和医用材料有限公司 | Medical self-adhesion dressing |
| CN103877608A (en) * | 2014-03-25 | 2014-06-25 | 南昌市意尔康医疗器械有限公司 | Surgical dressing containing chitin |
-
2016
- 2016-11-01 CN CN201610935998.3A patent/CN106362194A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8273702B2 (en) * | 2006-02-17 | 2012-09-25 | Wake Forest University Health Sciences | Wound healing compositions containing keratin biomaterials |
| CN101530636A (en) * | 2008-03-12 | 2009-09-16 | 章庆国 | Preparation method of injectable human hair keratin soft tissue filling material |
| CN202477971U (en) * | 2011-12-27 | 2012-10-10 | 四川三和医用材料有限公司 | Medical self-adhesion dressing |
| CN103877608A (en) * | 2014-03-25 | 2014-06-25 | 南昌市意尔康医疗器械有限公司 | Surgical dressing containing chitin |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108714243A (en) * | 2017-06-08 | 2018-10-30 | 重庆大学 | Implant and preparation method thereof for post operation of hypertensive cerebral hemorrhage hemostasis |
| CN107126305A (en) * | 2017-06-12 | 2017-09-05 | 大连医科大学附属第医院 | A kind of maggot debridement bag |
| CN111202868A (en) * | 2020-01-17 | 2020-05-29 | 重庆大学 | Composition for preparing keratin gel dressing, preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105311681B (en) | Injectable composite material for bone repair and preparation method thereof | |
| CN106132447A (en) | Bio-medical instrument | |
| EP1335018A1 (en) | Sericin-containing material, process for producing the same and method of using the same | |
| CN105797212A (en) | Preparing method and application of acellular amniotic membrane used for repairing skin wound difficult to heal | |
| CN106362194A (en) | Dressing for repairing wound surface and preparing method thereof | |
| CN104055795B (en) | A kind of injectable implant and preparation method thereof | |
| CN101385870B (en) | Method for improving de-cellular system engineering valve/blood vessel stent | |
| CN105521525B (en) | A kind of bone tissue engineer porous compound support frame and preparation method thereof | |
| CN111393521A (en) | Extraction method of jellyfish collagen | |
| CN101773687A (en) | Preparation method of composite soft-tissue patch | |
| CN109621010A (en) | A kind of Acellular cartilaginous matrix and preparation method thereof | |
| KR101429522B1 (en) | Method for extracting extracelluar matrix from placenta and scaffold for skin wound treatment using material extracted by the same | |
| CN102677392A (en) | Production technology of biochemical hydrophilic silk non-woven fabric | |
| CN105833330B (en) | A kind of preparation method of bamboo based nano-fiber element collagen composite material | |
| CN108969466A (en) | A kind of facial mask and preparation method thereof of preserving youthful looks based on the umbilical cord mesenchymal stem cells secretion activity factor | |
| CN103961752B (en) | Tissue regeneration guiding film and preparation method thereof | |
| CN109364298A (en) | A kind of preparation method of acellular dermal matrix material | |
| CN106421888A (en) | Keratin-chitosan composite medical dressing and preparation method thereof | |
| CN106474533A (en) | A kind of it is applied to combine dressing of the surface of a wound and preparation method thereof | |
| CN104721877B (en) | A kind of sterile collagen sticking dressing and preparation method thereof | |
| CN104189009A (en) | Small intestinal submucosa-based vascularization promoting thermosensitive material and preparation method thereof | |
| CN104971382B (en) | The mounted artificial active mass of wound and its construction method that a kind of use serum-free and ox pituitary extract nutrient solution are built | |
| CN106474527A (en) | A kind of preparation method of the engineered dressing of hair-keratin compound cells | |
| CN108042841A (en) | A kind of biological dressing and preparation method thereof and purposes | |
| CN106975099B (en) | Method for preparing collagen sponge |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170201 |