CN106359099A - Tissue culture management method based on NFC (near field communication) technology - Google Patents
Tissue culture management method based on NFC (near field communication) technology Download PDFInfo
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- CN106359099A CN106359099A CN201610873052.9A CN201610873052A CN106359099A CN 106359099 A CN106359099 A CN 106359099A CN 201610873052 A CN201610873052 A CN 201610873052A CN 106359099 A CN106359099 A CN 106359099A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention provides a tissue culture management method based on an NFC (near field communication) technology, and belongs to the technical filed of tissue culture management. An NFC chip and identification equipment are added on a tissue culture tool, so that in the overall tissue culture process comprising various stages from inoculating to taking out sound seedlings from a bottle and the like, recording and tracking regulation of the whole tissue culture process can be realized by means of the NFC technology and a computer system. The tissue culture management method based on an NFC technology has the advantages of modernization, informatization and easy management.
Description
Technical field
The invention belongs to tissue culture's management technology, more particularly, to a kind of tissue culture's management based on nfc mechanics of communication
Method.
Background technology
Plant tissue culture (broad sense) is isolated culture again, refers to isolate the tissue suiting the requirements, organ, thin from plant
Born of the same parents or protoplast etc., by sterile working, are cultivated whole plant or the life to obtain regeneration under manual control condition
Produce the technology of other products with economic worth.Plant tissue culture (narrow sense) refers to be organized with plant parts, such as cambium layer,
Parenchyma, mesophyll tissue, endosperm etc. carry out culture and obtain regeneration plant, also refer to produce from each organ in incubation and heal
The culture of injured tissue, calluss are again through being differentiated to form aftergrowth again.By tissue culture technique, plant is multiplied
The purpose that rapid multiplying can be reached and keep maternal plant merit, tissue culture technique is not limited by regions simultaneously,
Through being widely used among rapid multiplying, rareness species protection and the famous and precious botanical derivative of making of famous and precious plant.
Whether produce or scientific research, particularly factorial praluction, tissue culture is belonging to intensive work on a large scale, with
When often batch of tissue culture plant be required for into excessively multiple propagation, change culture medium, different kinds needs using different trainings
Foster base, even if identical kind is required for using different culture medium under different phase or different culture purposes, wherein
Management process extremely complex and loaded down with trivial details, tend to malfunction by manual record tissue culture information, and traditional method many
Paste label paper, recording-related information in tissue culture body, but label paper easily damages in tissue culture's operation or drops
And cause information loss, managing to tissue culture increases difficulty, simultaneously using the method manually pasting label paper on tissue culture bottle
Do not meet the development trend of modern agriculture, information-based agriculture.
Nfc near-field communication technology is to be developed by non-contact radio-frequency identification (rfid) and the Technology Integration that interconnects,
Combining induction card reader, icah wavw and point-to-point function on one chip, can be in short distance and compatible equipment
It is identified data to exchange.
Content of the invention
The problems referred to above are had based on prior art, the present invention provides a kind of management of tissue culture based on nfc mechanics of communication
Method, it passes through to the addition of nfc chip and identification equipment on the instrument of tissue culture, in tissue culture's overall process, including
From being inoculated into each stage such as strong sprout bottle outlet, adjusted entirely individual using nfc near-field communication technology and computer system record, tracking
Tissue culture procedures, a kind of tissue culture's management method based on nfc mechanics of communication that the present invention provides has modernization, information
The advantage change, being easily managed.
A kind of tissue culture's management method based on nfc mechanics of communication, it comprises the following steps:
Step s10 prepares culture medium, prepares culture medium, subpackage according to culture medium prescription and carries out high temperature sterilize, treats that culture medium is cold
But load onto nfc chip pad for tissue culture flasks after solidifying, train using nfc identification device scan and by corresponding for tissue culture bottle pairing
Foster base information；
Step s20 is inoculated, typing tissue culture information in advance, and tissue culture technology person uses the nfc device scan oneself on aseptic operating platform
ID card, corresponding culture medium is taken according to the plan of computer arrangement on nfc identification equipment, scans record, take plant
Explant is inoculated work accordingly, the tissue culture bottle after scanning inoculation finishes again after the completion of inoculation；
Manage between step s30 bottle, between bottle, management personnel check tissue culture plant daily, choose the tissue culture of contaminated or complete death
Bottle, scans record to contaminated and complete dead tissue culture bottle respectively on nfc identification equipment；
Step s40 is bred, and computer determines propagation number of times according to the growth coefficient of plant variety, and sends propagation information, tissue culture
Technician uses the nfc device scan ID card of oneself on aseptic operating platform, is taken according to propagation information and needs accordingly to increase
Grow batch, take out and need the tissue culture bottle of propagation to scan on nfc identification equipment and then carry out breeding sterile working, after propagation
New bottle reuses nfc identification device scan, and the information in old bottle is added in new bottle information, and between execution step s30 bottle
Management, until it reaches default proliferation times；
Step s50 goes out root, and after reaching default proliferation times, computer sends out root information, and tissue culture technology person uses aseptic
The nfc device scan ID card of oneself on operating board, being taken according to propagation information needs root batch accordingly, takes out and needs
The tissue culture bottle going out root scans on nfc identification equipment and then carries out Chu Gen sterile working, reuses nfc by changing the new bottle after bottle
Identification device scan, the information in old bottle is added in new bottle information, and manages between execution step s30 bottle；
Step s60 strong sprout, after reaching default proliferation times, computer sends strong sprout information, and tissue culture technology person uses aseptic
The nfc device scan ID card of oneself on operating board, being taken according to strong sprout information needs strong sprout batch accordingly, takes out and needs
The tissue culture bottle of strong sprout scans on nfc identification equipment and then carries out strong sprout sterile working, reuses nfc by changing the new bottle after bottle
Identification device scan, the information in old bottle is added in new bottle information, and manages between execution step s30 bottle.
Wherein, also need execution step s11 before described step s10, culture medium prescription that typing in advance will be prepared, join
Personal information processed and automatic record preparation time.
Wherein, step s20 inoculation in tissue culture information include species numbering, goods number, production quantity, the production time and
Propagation number of times.
Wherein, also include step s21 in described step s20 inoculation, computer calculates each according to the information of record
The workload of tissue culture technology person.
Wherein, also include step s31 in managing between described step s30 bottle, computer calculates according to the information of record
Kind pollution rate, mortality rate, technician's operational pollution rate.
Wherein, also include step s32 bottle outlet in managing between described step s30 bottle, the tissue cultured seedling after strong sprout reaches
Can with bottle outlet when, between bottle, management personnel take out and can identify device scan record bottle outlet information using nfc with the tissue cultured seedling of bottle outlet,
After scanned, Seedling is transferred between seedling exercising.
Wherein, inoculate the no root of quantification in each tissue culture bottle in described step s40 propagation, computer is according to group
Training bottle quantity converses plant generation number.
Wherein, it adopts the identification of nfc mechanics of communication, each bottle seedling of recording and tracking in whole tissue culture procedures, makes
With the real-time record information of computer, calculate the work efficiency of tissue culture technology person, pollution rate, volume of production and to manage control whole
Individual tissue culture procedures.
Beneficial effects of the present invention: identify and follow the tracks of each bottle of tissue cultured seedling by nfc near-field communication technology, recycle and calculate
The information collected is counted by machine software, calculate and produce or scientific research required for data, such as pollution rate, teething rate and
Value-added coefficient, production efficiency, production quantity etc..Avoid because tissue cultured seedling quantity is too many and cause artificial missing error.
Specific embodiment
With reference to specific embodiment, the invention will be further described.
A kind of tissue culture's management method based on nfc mechanics of communication is it is characterised in that it comprises the following steps:
Step s10 prepares culture medium, when culture medium prescription, formulator's information and the automatic record that typing will be prepared is prepared
Between, culture medium, subpackage are prepared according to culture medium prescription and carries out high temperature sterilize, be tissue culture flasks after culture medium cooled and solidified
Load onto nfc chip pad, match corresponding culture medium information using nfc identification device scan and by tissue culture bottle.
Step s20 is inoculated, in advance the group such as typing species numbering, goods number, production quantity, production time and propagation number of times
Training information, tissue culture technology person uses the nfc device scan ID card of oneself on aseptic operating platform, according to the meter of computer arrangement
Draw and take corresponding culture medium and scan record on nfc identification equipment, take plant explant and inoculated work accordingly, connect
Tissue culture bottle after scanning inoculation finishes again after the completion of kind, computer calculates each tissue culture technology person's according to the information of record
Workload.
Manage between step s30 bottle, between bottle, management personnel check tissue culture plant daily, choose contaminated or complete death
Tissue culture bottle, scans record to contaminated and complete dead tissue culture bottle, computer is according to record respectively on nfc identification equipment
Information calculates kind pollution rate, mortality rate, technician's operational pollution rate, and the tissue cultured seedling after strong sprout reaches can be with bottle outlet
When, between bottle, management personnel take out and can identify device scan record bottle outlet information with the tissue cultured seedling of bottle outlet using nfc, scanned
Afterwards Seedling is transferred between seedling exercising.
Step s40 is bred, and computer determines propagation number of times according to the growth coefficient of plant variety, and sends propagation information,
Tissue culture technology person uses the nfc device scan ID card of oneself on aseptic operating platform, takes corresponding need according to propagation information
Batch to be bred, takes out and needs the tissue culture bottle of propagation to scan on nfc identification equipment and then carry out breeding sterile working, each group
The no root of quantification is inoculated, computer converses plant generation number according to tissue culture bottle quantity, after propagation in training bottle
New bottle reuses nfc identification device scan, and the information in old bottle is added in new bottle information, and between execution step s30 bottle
Management, until it reaches default proliferation times.
Step s50 goes out root, and after reaching default proliferation times, computer sends out root information, and tissue culture technology person uses
The nfc device scan ID card of oneself on aseptic operating platform, being taken according to propagation information needs root batch accordingly, takes out
Need the tissue culture bottle of root to scan on nfc identification equipment and then carry out Chu Gen sterile working, make changing the new bottle after bottle again
Identify device scan with nfc, the information in old bottle is added in new bottle information, and manage between execution step s30 bottle.
Step s60 strong sprout, after reaching default proliferation times, computer sends strong sprout information, and tissue culture technology person uses
The nfc device scan ID card of oneself on aseptic operating platform, being taken according to strong sprout information needs strong sprout batch accordingly, takes out
Need the tissue culture bottle of strong sprout to scan on nfc identification equipment and then carry out strong sprout sterile working, make changing the new bottle after bottle again
Identify device scan with nfc, the information in old bottle is added in new bottle information, and manage between execution step s30 bottle.
Adopt the identification of nfc mechanics of communication, each bottle seedling of recording and tracking in whole tissue culture procedures, using computer
Real-time record information, calculates the work efficiency of tissue culture technology person, pollution rate, volume of production and manages control whole tissue training
Foster flow process.
Embodiment described above only have expressed one embodiment of the present invention, and its description is more concrete and detailed, but simultaneously
Therefore the restriction to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, some deformation can also be made and improve, these broadly fall into the guarantor of the present invention
Shield scope.Therefore, the protection domain of patent of the present invention should be defined by claims.
Claims (8)
1. a kind of tissue culture's management method based on nfc mechanics of communication is it is characterised in that it comprises the following steps:
Step s10 prepares culture medium, prepares culture medium, subpackage according to culture medium prescription and carries out high temperature sterilize, treats that culture medium is cold
But load onto nfc chip pad for tissue culture flasks after solidifying, train using nfc identification device scan and by corresponding for tissue culture bottle pairing
Foster base information；
Step s20 is inoculated, typing tissue culture information in advance, and tissue culture technology person uses the nfc device scan oneself on aseptic operating platform
ID card, corresponding culture medium is taken according to the plan of computer arrangement on nfc identification equipment, scans record, take plant
Explant is inoculated work accordingly, the tissue culture bottle after scanning inoculation finishes again after the completion of inoculation；
Manage between step s30 bottle, between bottle, management personnel check tissue culture plant daily, choose the tissue culture of contaminated or complete death
Bottle, scans record to contaminated and complete dead tissue culture bottle respectively on nfc identification equipment；
Step s40 is bred, and computer determines propagation number of times according to the growth coefficient of plant variety, and sends propagation information, tissue culture
Technician uses the nfc device scan ID card of oneself on aseptic operating platform, is taken according to propagation information and needs accordingly to increase
Grow batch, take out and need the tissue culture bottle of propagation to scan on nfc identification equipment and then carry out breeding sterile working, after propagation
New bottle reuses nfc identification device scan, and the information in old bottle is added in new bottle information, and between execution step s30 bottle
Management, until it reaches default proliferation times；
Step s50 goes out root, and after reaching default proliferation times, computer sends out root information, and tissue culture technology person uses aseptic
The nfc device scan ID card of oneself on operating board, being taken according to propagation information needs root batch accordingly, takes out and needs
The tissue culture bottle going out root scans on nfc identification equipment and then carries out Chu Gen sterile working, reuses nfc by changing the new bottle after bottle
Identification device scan, the information in old bottle is added in new bottle information, and manages between execution step s30 bottle；
Step s60 strong sprout, after reaching default proliferation times, computer sends strong sprout information, and tissue culture technology person uses aseptic
The nfc device scan ID card of oneself on operating board, being taken according to strong sprout information needs strong sprout batch accordingly, takes out and needs
The tissue culture bottle of strong sprout scans on nfc identification equipment and then carries out strong sprout sterile working, reuses nfc by changing the new bottle after bottle
Identification device scan, the information in old bottle is added in new bottle information, and manages between execution step s30 bottle.
2. a kind of tissue culture's management method based on nfc mechanics of communication according to claim 1 is it is characterised in that walk
Execution step s11 is also needed, culture medium prescription, formulator's information and automatic record that typing in advance will be prepared before rapid s10
The preparation time.
3. a kind of tissue culture's management method based on nfc mechanics of communication according to claim 1 is it is characterised in that walk
Tissue culture information in rapid s20 inoculation includes species numbering, goods number, production quantity, production time and propagation number of times.
4. a kind of tissue culture's management method based on nfc mechanics of communication according to claim 1 is it is characterised in that walk
Step s21 is also included, computer calculates the workload of each tissue culture technology person according to the information of record in rapid s20 inoculation.
5. a kind of tissue culture's management method based on nfc mechanics of communication according to claim 1 is it is characterised in that walk
Step s31 is also included, computer calculates kind pollution rate, mortality rate, technology according to the information of record in managing between rapid s30 bottle
Member's operational pollution rate.
6. a kind of tissue culture's management method based on nfc mechanics of communication according to claim 1 is it is characterised in that walk
Also include step s32 bottle outlet in managing between rapid s30 bottle, the tissue cultured seedling after strong sprout reach can with bottle outlet when, administrative man between bottle
Member takes out and can identify device scan record bottle outlet information using nfc with the tissue cultured seedling of bottle outlet, scanned after Seedling is transferred to refining
Miao Jian.
7. a kind of tissue culture's management method based on nfc mechanics of communication according to claim 1 is it is characterised in that walk
The no root of quantification is inoculated, computer converses plant according to tissue culture bottle quantity and increases in each tissue culture bottle in rapid s40 propagation
Grow a number.
8. according to claim 1 a kind of based on nfc mechanics of communication tissue culture management method it is characterised in that its
The identification of nfc mechanics of communication, each bottle seedling of recording and tracking is adopted in whole tissue culture procedures, real-time using computer
Record information, calculates the work efficiency of tissue culture technology person, pollution rate, volume of production and manages the whole tissue culture procedures of control.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610873052.9A CN106359099A (en) | 2016-10-08 | 2016-10-08 | Tissue culture management method based on NFC (near field communication) technology |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610873052.9A CN106359099A (en) | 2016-10-08 | 2016-10-08 | Tissue culture management method based on NFC (near field communication) technology |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103606008A (en) * | 2013-11-05 | 2014-02-26 | 福州市勘测院 | Method based on RFID plant transplanting management and system thereof |
| CN103782954A (en) * | 2014-01-25 | 2014-05-14 | 广东燕塘乳业股份有限公司 | Cow fine breeding method based on RFID technology |
| CN105590081A (en) * | 2016-03-15 | 2016-05-18 | 广州佰迈起生物科技有限公司 | Radio frequency identification tag read system, read-write device and read method |
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2016
- 2016-10-08 CN CN201610873052.9A patent/CN106359099A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103606008A (en) * | 2013-11-05 | 2014-02-26 | 福州市勘测院 | Method based on RFID plant transplanting management and system thereof |
| CN103782954A (en) * | 2014-01-25 | 2014-05-14 | 广东燕塘乳业股份有限公司 | Cow fine breeding method based on RFID technology |
| CN105590081A (en) * | 2016-03-15 | 2016-05-18 | 广州佰迈起生物科技有限公司 | Radio frequency identification tag read system, read-write device and read method |
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Application publication date: 20170201 |