CN106350467A - Strain capable of reducing nicotine in tobacco leaves, and screening method, culturing method and application thereof - Google Patents
Strain capable of reducing nicotine in tobacco leaves, and screening method, culturing method and application thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12R2001/085—Bacillus cereus
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a strain capable of reducing nicotine in tobacco leaves. The strain is bacillus cereus, and is obtained in the following ways: adding 9-10 g of a soil sample into 90 mL of an enrichment culture medium, and culturing for 40-50 h at the temperature of 28-32 DEG C to obtain enrichment culture liquid; adding 1-1.2 mL of the enrichment culture liquid into a culture dish with an isolated culture medium molten and cooled to 48-52 DEG C, solidifying, putting into a constant-temperature culture box with the temperature of 28-32 DEG C, and culturing for 6-8 days; selecting a growing bacterial colony, inoculating a domestication culture medium with the bacterial colony, and culturing for 90-110 h under the conditions that the temperature is 28-32 DEG C and the rotting speed is 100-150 rpm; then diluting a culture in a gradient way, coating a flat plate with the isolated culture medium with the culture to obtain single bacterial colonies, and separating and purifying by streaking. After the strain is used for fermenting the tobacco leaves with a high nicotine content and a seriously-imbalanced sugar-nicotine ratio, the nicotine content can be reduced by 20-40%.
Description
Technical field
The present invention relates to a kind of microbial strains are and in particular to a kind of Bacillus cercuses reducing nicotine in Nicotiana tabacum L.
(bacillus cereus), and its screening technique, the application in Nicotiana tabacum L. of cultural method and this bacterial strain.
Background technology
Nicotiana tabacum L. is the raw material of production of cigarettes, and the quality of quality of tobacco is directly connected to the height of cigarette quality.The change of Nicotiana tabacum L.
Learn conventional ingredient to be mainly: total alkaloid, Water-soluble Total Sugar, reducing sugar, total nitrogen, chlorine and potassium, also contain protein, starch, fibre
Dimension element, the macromolecular compound such as pectin, these compositions in the content in Nicotiana tabacum L. and the proportionate relationship between some compositions, to cigarette
Leaf quality has important impact.Nicotine also known as nicotine (nicotine), are the main components in nicotiana alkaloidss.In flue-cured tobacco
The content of nicotine, typically between 1.5%～3.5%, is advisable with 2.5% about.Nicotine content is too low, and strength is too little, lacks full
Foot is felt, and nicotine content is too high, and strength is too big, and thorn can be caused to choke pungent sense.China's part stock's Nicotiana tabacum L. (particularly upper tobacco leaf)
There is nicotine content too high, sugared alkali is than numerous imbalances, the quality of Nicotiana tabacum L. and the problem of poor availability.
At present, the method reducing nicotine content in tobacco leaf mainly has three kinds of approach: 1. from agriculturals such as heredity, ecological, plantations
Plantation aspect carries out nicotine content regulation and control；2. hot water rinsing, organic solvent extraction, gas extracting, steam distillation etc. is adopted to process
Nicotiana tabacum L., chemically nicotine in angle removing Nicotiana tabacum L.；3. adopt microorganism or biotechnology of enzymes to process Nicotiana tabacum L., reduce nicotine in Nicotiana tabacum L.
Content, improves the availability of Nicotiana tabacum L..For the Nicotiana tabacum L. that maturation has harvested, can only be using two kinds of approach below.Wherein, adopt
Though chemical method can remove nicotine, the loss of some fragrance matters in Nicotiana tabacum L. can be led to simultaneously, and pass through microorganism or enzyme
Process Nicotiana tabacum L., because enzyme has high specificity, thus can preferably avoid a difficult problem for Nicotiana tabacum L. fragrance component damages.
Content of the invention
Too high for some stock's nicotine content in tobacco leafs, sugared alkali than numerous imbalances, the quality of Nicotiana tabacum L. and poor availability
Problem, the present invention provides a kind of Bacillus cercuses (bacillus cereus) reducing nicotine in Nicotiana tabacum L.；
Another object of the present invention is to provide the screening side of described Bacillus cercuses (bacillus cereus)
Method；
A further object of the present invention there is provided the cultural method of Bacillus cercuses (bacillus cereus)；
A further object of the present invention there is provided Bacillus cercuses (bacillus cereus) answering in Nicotiana tabacum L.
With.
In order to reach above-mentioned technique effect, the present invention takes technical scheme below:
The bacterial strain of nicotine in a kind of reduction Nicotiana tabacum L., described bacterial strain is Bacillus cercuses (bacillus cereus), should
Bacterial strain is preserved in China General Microbiological culture presevation administrative center, and deposit number is: cgmcc 10338.
The invention provides the culture of described Bacillus cercuses and micro-morphology are as follows:
On nutrient agar flat board, bacterium colony is big, coarse, and irregularly, there are whiplike branch, micro white in edge, and surface has characteristic
Speckle；
Grow vigorous on nutrient agar inclined-plane, coarse, opaque, pale, extension, edge is irregular, has whiplike branch；
Cell is shaft-like, 3.0-4.8 × 1.0-1.1um, end side, becomes short chain.
The present invention also very provides the described screening technique reducing the bacterial strain of nicotine in Nicotiana tabacum L., comprises the following steps:
Step a: take pedotheque 9～10g in 90ml enrichment medium, then at 28～32 DEG C of temperature culture 40～
50h obtains enrichment culture liquid；
Step b: take enrichment culture liquid 1～1.2ml, put into equipped with the isolation medium being cooled to 48～52 DEG C after fusing
Culture dish, is placed in after solidification in the constant incubator of 28～32 DEG C of temperature and cultivates 6～8 days；Then the bacterium colony selecting growth accesses
In domestication culture medium, cultivate 90～110h under conditions of 28～32 DEG C of temperature, rotating speed 100～150rpm；Then by culture
Gradient dilution coats isolation medium flat board, obtains single bacterium colony, is isolated and purified by line and obtains described wax-like spore bar
Bacterium (bacillus cereus).
In above-mentioned screening technique, described enrichment medium includes the component of following weight: peptone, 5.0g；
Nacl, 1.0g；k2hpo4, 1.0g；Agar, 1.5g；The ph of enrichment medium is 7.0,121 DEG C of sterilizing 30min, adds after cooling
Nicotine 2.0g.
In above-mentioned screening technique, described isolation medium includes the component of following weight or volume: k2hpo4,
1.0g；mgso4, 0.5g；Mass fraction is 0.1% mnso4, 1ml；Mass fraction is 0.1% feso4, 3ml；Agar,
1.5g；6.0～7.0,121 DEG C of sterilizing 30min of the ph of isolation medium, add nicotine 4.0g after cooling.
In above-mentioned screening technique, described domestication culture medium includes the component of following weight or volume: k2hpo4,
1.0g；mgso4, 0.5g；Mass fraction is 0.1% mnso4, 1ml；Mass fraction is 0.1% feso4, 3ml；Domestication culture
6.0～7.0,121 DEG C of sterilizing 30min of the ph of base, add nicotine 2000mg after cooling.
Present invention also offers the described cultural method reducing the bacterial strain of nicotine in Nicotiana tabacum L., comprise the following steps:
Step a: slant culture: the Bacillus cercuses of freezen protective are inoculated on slant medium, in temperature 28～
Quiescent culture 1～2 day at 32 DEG C；
Step b: shaking flask liquid culture: the strain that step a is cultivated gained is inoculated on fluid medium, in temperature 28～
32 DEG C, shake-flask culture 18～24h under conditions of rotating speed 140～160r/min, cultivate and reach 10 to bacterium number10More than, obtain seed
Liquid.
In above-mentioned cultural method, described slant medium or fluid medium include the group of following weight or volume
Point: peptone 10g, Carnis Bovis seu Bubali cream 3g, sodium chloride 5g, agar 15g, distilled water 1000ml, ph value 7.0～7.2.
Present invention also offers the application of the described bacterial strain nicotine in reducing Nicotiana tabacum L. reducing nicotine in Nicotiana tabacum L., by seed
Liquid, according to the 5～30% of tobacco leaf weight, is added in Nicotiana tabacum L., then in 20～45 DEG C of bottom fermentations of temperature 1～6 day.
In the applications described above it is preferred that by seed liquor according to the 20～30% of tobacco leaf weight, being added in Nicotiana tabacum L.,
Then in 20～45 DEG C of bottom fermentations of temperature 1～6 day.
The invention provides one plant of Bacillus cercus (bacillus cereus), this strain growth reproductive capacity is strong, mycelia
Growth is vigorous.Process that nicotine content is high using this strain fermentation, sugared alkali than numerous imbalances Nicotiana tabacum L., can reduce nicotine content 20～
40%, there is preferable stability and repeatability, after fermentation, Nicotiana tabacum L. strength is moderate, the peppery sense of thorn of choking reduces, and harmony improves, sense organ
Quality is obviously improved.
Brief description
Fig. 1 is the colonial morphology figure of Bacillus cercuses (bacillus cereus)；
Fig. 2 is the cellular morphology figure of Bacillus cercuses (bacillus cereus)；
The preservation explanation of Bacillus cercuses (bacillus cereus):
Depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, abbreviation cgmcc；Preservation ground
Location: Chaoyang District Beijing North Star West Road 1 institute 3；
Deposit number: cgmcc no.10338；
The preservation time: on January 12nd, 2015.
Specific embodiment
With reference to embodiments of the invention, the invention will be further elaborated.
Embodiment 1: the separation of Bacillus cercuses, identification
Take pedotheque (Sichuan Liangshan District Rhizoma Zingiberis Recens state vega soil) 10g in 90ml enrichment medium, 30 DEG C of quiescent culture
48h.Take enrichment culture liquid 1ml, put into and be cooled to mix homogeneously in the culture dish of 50 DEG C of isolation mediums equipped with after fusing, solidification
After be put in 30 DEG C of constant incubators and cultivate 7 days.The bacterium colony of picking growth accesses in domestication culture medium, and 30 DEG C, 120rpm cultivates
96h, culture energy gradient dilution is coated isolation medium flat board, obtains single bacterium colony, isolates and purifies wax-like by line
Bacillus cereuss strain (bacillus cereus).
Described enrichment medium is: peptone, 5.0g；Nacl, 1.0g；k2hpo4, 1.0g；Agar, 1.5g；Enrichment culture
The ph of base is 7.0,121 DEG C of sterilizing 30min, adds nicotine 2.0g after cooling.
Described isolation medium is: k2hpo4, 1.0g；mgso4, 0.5g；Mass fraction is 0.1% mnso4, 1ml；Matter
Amount fraction is 0.1% feso4, 3ml；Agar, 1.5g；6.0～7.0,121 DEG C of sterilizing 30min of the ph of isolation medium are cold
But add nicotine 4.0g afterwards.
Described domestication culture medium is: k2hpo4, 1.0g；mgso4, 0.5g；Mass fraction is 0.1% mnso4, 1ml；Matter
Amount fraction is 0.1% feso4, 3ml；6.0～7.0,121 DEG C of sterilizing 30min of the ph of domestication culture medium, add cigarette after cooling
Alkali 2000mg.
The culture of said waxy bacillus cereuss and micro-morphology are described as follows:
On nutrient agar flat board, bacterium colony is big, coarse, and irregularly, there are whiplike branch, micro white in edge, and surface has characteristic
Speckle.
Grow vigorous on nutrient agar inclined-plane, coarse, opaque, pale, extension, edge is irregular, has whiplike branch,
Cell is shaft-like, 3.0-4.8 × 1.0-1.1um, end side, becomes short chain.
Identified, 16s rrna gene order and danj gene order that Bacillus cercuses have.
Embodiment 2: the preparation of Bacillus cercuses seed liquor
The Bacillus cercuses bacterium colony that embodiment 1 is obtained is enlarged cultivating and produces, and obtains Bacillus cercuses kind
Sub- liquid.
A, slant culture: peptone 10g, Carnis Bovis seu Bubali cream 3g, sodium chloride 5g, agar 15g, distilled water 1000ml, ph value 7.0～
7.2, the Bacillus cercuses strain (bacillus cereus) of freezen protective is inoculated on slant medium, 30 DEG C of constant temperature are quiet
Only culture 1-2 days.
B, shaking flask liquid culture: peptone 10g, Carnis Bovis seu Bubali cream 3g, sodium chloride 5g, agar 15g, distilled water 1000ml, ph value
7.0～7.2, high pressure steam sterilization, by step a thalline be inoculated in medium liquid, cultivation temperature: 30 DEG C, shaking table turns
Fast 140-160r/min, shake-flask culture 18-24h, cultivate and reach 10 to bacterium number10More than, obtain seed liquor.
Embodiment 3:
The Bacillus cercuses seed liquor that embodiment 2 is obtained, according to the 30% of tobacco leaf weight, is added to water content 15%
Nicotiana tabacum L. in, 25 DEG C of condition bottom fermentations 6 days, compared with non-fermentation process blank Nicotiana tabacum L., in fermenting tobacco leaf, nicotine content reduces
24.9%, Analyses Methods for Sensory Evaluation Results shows: fermenting tobacco leaf strength substantially reduces, zest significantly reduces, flue gas more alcohol and.
Embodiment 4:
The Bacillus cercuses seed liquor that embodiment 2 is obtained, according to the 5% of tobacco leaf weight, is added to water content 50%
In Nicotiana tabacum L., ferment 3 days, compared with non-fermentation process blank Nicotiana tabacum L., in fermenting tobacco leaf, nicotine content reduces by 29.7%, sensory evaluation
Result shows: fermenting tobacco leaf strength substantially reduces, zest significantly reduces, flue gas more alcohol and.
Embodiment 5:
The Bacillus cercuses seed liquor that embodiment 2 is obtained, according to the 20% of tobacco leaf weight, is added to water content 35%
Nicotiana tabacum L. in, ferment 4 days, with non-fermentation process blank Nicotiana tabacum L. compared with, in fermenting tobacco leaf nicotine content reduce by 38.1%, sense organ is commented
Valency result shows: fermenting tobacco leaf strength substantially reduces, zest significantly reduces, flue gas more alcohol and.
Although reference be made herein to invention has been described for the explanatory embodiment of the present invention, and above-described embodiment is only this
Bright preferably embodiment, embodiments of the present invention are simultaneously not restricted to the described embodiments it should be appreciated that people in the art
Member can be designed that a lot of other modifications and embodiment, and these modifications and embodiment will fall in principle disclosed in the present application
Within scope and spirit.
Claims (10)
1. a kind of bacterial strain reducing nicotine in Nicotiana tabacum L. is it is characterised in that described bacterial strain is Bacillus cercuses (bacillus
Cereus), this bacterial strain is preserved in China General Microbiological culture presevation administrative center, and deposit number is: cgmcc 10338.
2. the bacterial strain reducing nicotine in Nicotiana tabacum L. according to claim 1 is it is characterised in that described Bacillus cercuses
Culture and micro-morphology are as follows:
On nutrient agar flat board, bacterium colony is big, coarse, and irregularly, there are whiplike branch, micro white in edge, and surface has characteristic speckle
Stricture of vagina；
Grow vigorous on nutrient agar inclined-plane, coarse, opaque, pale, extension, edge is irregular, has whiplike branch；Cell
Shaft-like, 3.0-4.8 × 1.0-1.1um, end side, become short chain.
3. described in claim 1 reduce Nicotiana tabacum L. in nicotine bacterial strain screening technique it is characterised in that comprising the following steps:
Step a: take pedotheque 9～10g in 90ml enrichment medium, then cultivate 40～50h at 28～32 DEG C of temperature
Obtain enrichment culture liquid；
Step b: take enrichment culture liquid 1～1.2ml, put into the culture equipped with the isolation medium being cooled to 48～52 DEG C after fusing
Ware, is placed in after solidification in the constant incubator of 28～32 DEG C of temperature and cultivates 6～8 days；Then the bacterium colony selecting growth accesses domestication
In culture medium, cultivate 90～110h under conditions of 28～32 DEG C of temperature, rotating speed 100～150rpm；Then by culture gradient
Dilution spread, in isolation medium flat board, obtains single bacterium colony, is isolated and purified by line and obtains described Bacillus cercuses
(bacillus cereus).
4. the screening technique reducing the bacterial strain of nicotine in Nicotiana tabacum L. according to claim 3 is it is characterised in that described enrichment
Culture medium includes the component of following weight: peptone, 5.0g；Nacl, 1.0g；k2hpo4, 1.0g；Agar, 1.5g；Enrichment culture
The ph of base is 7.0,121 DEG C of sterilizing 30min, adds nicotine 2.0g after cooling.
5. the screening technique reducing the bacterial strain of nicotine in Nicotiana tabacum L. according to claim 3 is it is characterised in that described separation
Culture medium includes the component of following weight or volume: k2hpo4, 1.0g；mgso4, 0.5g；Mass fraction is 0.1% mnso4,
1ml；Mass fraction is 0.1% feso4, 3ml；Agar, 1.5g；6.0～7.0,121 DEG C of sterilizings of the ph of isolation medium
30min, adds nicotine 4.0g after cooling.
6. the screening technique reducing the bacterial strain of nicotine in Nicotiana tabacum L. according to claim 3 is it is characterised in that described domestication
Culture medium includes the component of following weight or volume: k2hpo4, 1.0g；mgso4, 0.5g；Mass fraction is 0.1% mnso4,
1ml；Mass fraction is 0.1% feso4, 3ml；6.0～7.0,121 DEG C of sterilizing 30min of the ph of domestication culture medium, after cooling
Add nicotine 2000mg.
7. described in claim 1 reduce Nicotiana tabacum L. in nicotine bacterial strain cultural method it is characterised in that comprising the following steps:
Step a: slant culture: the Bacillus cercuses of freezen protective are inoculated on slant medium, in 28～32 DEG C of temperature
Lower quiescent culture 1～2 day；
Step b: shaking flask liquid culture: the strain that step a is cultivated gained is inoculated on fluid medium, in temperature 28～32
DEG C, shake-flask culture 18～24h under conditions of rotating speed 140～160r/min, cultivate and reach 10 to bacterium number10More than, obtain seed liquor.
8. the cultural method reducing the bacterial strain of nicotine in Nicotiana tabacum L. according to claim 7 is it is characterised in that described inclined-plane
Culture medium or fluid medium include the component of following weight or volume: peptone 10g, Carnis Bovis seu Bubali cream 3g, sodium chloride 5g, agar
15g, distilled water 1000ml, ph value 7.0～7.2.
9. the application of the bacterial strain nicotine in reducing Nicotiana tabacum L. reducing nicotine in Nicotiana tabacum L. described in claim 7 is it is characterised in that incite somebody to action
Seed liquor, according to the 5～30% of tobacco leaf weight, is added in Nicotiana tabacum L., then in 20～45 DEG C of bottom fermentations of temperature 1～6 day.
10. the application of bacterial strain according to claim 9 nicotine in reducing Nicotiana tabacum L. is it is characterised in that by seed liquor according to cigarette
The 20～30% of leaf weight, are added in Nicotiana tabacum L., then in 20～45 DEG C of bottom fermentations of temperature 1～6 day.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10405571B2 (en) | 2015-06-26 | 2019-09-10 | Altria Client Services Llc | Compositions and methods for producing tobacco plants and products having altered alkaloid levels |
| CN113215062A (en) * | 2021-06-15 | 2021-08-06 | 四川中烟工业有限责任公司 | Bacillus amyloliquefaciens, and acquisition method and application thereof |
| CN115191636A (en) * | 2022-06-03 | 2022-10-18 | 云南瑞升烟草技术（集团）有限公司 | Reconstituted tobacco with high sugar-alkali ratio and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102168051A (en) * | 2011-01-25 | 2011-08-31 | 浙江工业大学 | Bacillus cereus with higher resistance to jinggangmycin aqua and application |
| CN102266118A (en) * | 2011-06-03 | 2011-12-07 | 川渝中烟工业公司 | Preparation method of tobacco leaves of cigars |
| CN103082402A (en) * | 2013-02-06 | 2013-05-08 | 川渝中烟工业有限责任公司 | Wettable powder for cigar tobacco fermentation |
-
2016
- 2016-08-31 CN CN201610786460.0A patent/CN106350467B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102168051A (en) * | 2011-01-25 | 2011-08-31 | 浙江工业大学 | Bacillus cereus with higher resistance to jinggangmycin aqua and application |
| CN102266118A (en) * | 2011-06-03 | 2011-12-07 | 川渝中烟工业公司 | Preparation method of tobacco leaves of cigars |
| CN103082402A (en) * | 2013-02-06 | 2013-05-08 | 川渝中烟工业有限责任公司 | Wettable powder for cigar tobacco fermentation |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10405571B2 (en) | 2015-06-26 | 2019-09-10 | Altria Client Services Llc | Compositions and methods for producing tobacco plants and products having altered alkaloid levels |
| CN113215062A (en) * | 2021-06-15 | 2021-08-06 | 四川中烟工业有限责任公司 | Bacillus amyloliquefaciens, and acquisition method and application thereof |
| CN115191636A (en) * | 2022-06-03 | 2022-10-18 | 云南瑞升烟草技术（集团）有限公司 | Reconstituted tobacco with high sugar-alkali ratio and preparation method thereof |
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| CN106350467B (en) | 2019-08-09 |
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