CN106349303A - Preparation method of ganglioside extract - Google Patents
Preparation method of ganglioside extract Download PDFInfo
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- CN106349303A CN106349303A CN201610744824.9A CN201610744824A CN106349303A CN 106349303 A CN106349303 A CN 106349303A CN 201610744824 A CN201610744824 A CN 201610744824A CN 106349303 A CN106349303 A CN 106349303A
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- ganglioside
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/02—Acyclic radicals, not substituted by cyclic structures
- C07H15/04—Acyclic radicals, not substituted by cyclic structures attached to an oxygen atom of the saccharide radical
- C07H15/10—Acyclic radicals, not substituted by cyclic structures attached to an oxygen atom of the saccharide radical containing unsaturated carbon-to-carbon bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
Abstract
A preparation method of ganglioside extract includes subjecting fresh pig brains to microwave vacuum drying treatment to obtain dry pig brains with moisture content lower than 5wt%, and grinding the dry pig brains into pig brain powder, wherein the vacuum degree for microwave vacuum drying ranges from 0.04 Mpa to 0.09 Mpa; adding absolute ethyl alcohol into the dry pig brain powder, stirring for 2.0-5.0 hours at 35-55 DEG C, and filtering to obtain precipitate I; adding a mixed solvent of an acetic acid-sodium acetate buffer solution with a pH being 5.0-5.4, isopropanol and dichloromethane into the precipitate I, stirring for 60-120 minutes at a room temperature, and conducting centrifugal separation to obtain liquid supernatant I; cooling the liquid supernatant I to 15-25 DEG C to enable precipitate II to separate out, and filtering to obtain the precipitate II; repeatedly washing the precipitate II by anhydrous ether prior to filtration and vacuum drying so as to obtain the ganglioside extract. The preparation method of the ganglioside extract has the advantages that consumption of harmful solvents is reduced substantially, and the prepared ganglioside extract is high in ganglioside purity and GM1 content.
Description
Background technology
Ganglioside is glycosyl sphingolipid the most complicated, is present in the almost all of tissue of vertebratess microly, but
Content highest in the brain of mammal and myeloid tissue.There is the enhancing growth of human brain function and the function of hypermnesis, god
Warp knuckle glycosides fat is also the information regulatory factor in nervous system, and it, in terms for the treatment of and biological protection, has obvious curative effect
And effect.In mammal brain and myeloid tissue, main ganglioside is gm1, gd1b, gd1a and gt1b etc..Ganglioside
The physiological function of fat molecule specifically includes that 1) as the double-deck component with cell glycocalyx of membrane lipid；2) maintain film surface negative charge；
3) cell recognition and signal transmission are participated in；4) protection ischemia (oxygen) property nervous lesion etc..
Biomembranous basic structure is double-layer of lipoid, according to the difference of lipid framing structure, these lipids can be divided into fat
Fat, phosphoglyceride, sphingomyelins, glycolipid and steroid five big class.Glycolipid is even different according to fat, is further divided into glycerol and derives
Glyceroglycolipid, glycosyl sphingolipid derived from glycolipid, sphingosine derived from glycolipid, dolichol derived from steroid.Ganglioside
Fat belongs to glycosyl sphingolipid, has a common mesh nuclear structure ceramide.So far separated identify more than 70 kind.Single saliva
Sour tetrahexose ganglioside gm1It is the main species of mammalss ganglioside.
Monostalotetrahexosylgangliside (gm1) it is proved to that there is significantly neural maintenance and regeneration activity, it is god
Good medicine through degenerative disease or nerve injury, has been applied to Alzheimer's disease, Parkinson's disease etc. at present
In the treatment of disease.Due to the restriction of production technology, gm1Price very expensive.
Prior art discloses numerous preparation gm1Method, Chinese patent application discloses No. 102020684a, open
A kind of Monostalotetrahexosylgangliside (gm1) preparation method, it is divided into four steps:
The first step: the extraction of total fat, with Medulla sus domestica, Medulla Bovis seu Bubali, Babalus bubalis L. brain etc. as raw material, after homogenate, use methanol and chloroform mixing molten
Agent is extracted, and supernatant adds water and the hydrolysis of 0.2%naoh solution, obtains aqueous phase crude extract after layering.
Second step: gross porosity resins exchange post separation, crude extract macroporous resin adsorption, wash with water after saturation to clarification,
Then desorbing, solution sample liquid uses acetone precipitation after concentrating, and obtains ganglioside mixture.
3rd step: silica gel column chromatography, ganglioside mixture silica gel column chromatography, collect gm1Part, concentrated fullness of vitality
Warp knuckle glycosides fat (gm1) crude product.
4th step: ion exchange resin column separates, gm1After concentrated solution dissolving, upper cation exchange resin column, uses 5% salt
Acid, methanol-eluted fractions, then distillation, concentration, then with acetone precipitation, it is vacuum dried to obtain gm1Highly finished product.
Chinese patent application discloses No. 103951715a, discloses monosialoganglioside gm1And its separate and
Purification process, particularly to the monosialoganglioside gm in pig source1, it is according to following purification single sialic acid ganglioside
Fat gm1Method preparation, methods described includes: using the eluent containing potassium or cesium ion pass through ion exchange column chromatography,
From containing monosialoganglioside gm1In lipid mixture as Main Gangliosides component, by single sialic acid god
Warp knuckle glycosides fat gm1With fucose acyl gm1Separate.
In existing method, gm is separated from many gangliosides by chromatography method1, technical process is more multiple
Miscellaneous, along with gm in the total fat of original ganglioside1Content just ratio is relatively low, then, the gm being put forward by complicated technology1Amount
It is fewer, compare cost of idleness.If when proposing ganglioside total fat extract, gm can be improved1Content,
Can also be gm1The extraction of amount is cost-effective, and in other words, same extracting method obtains gm1Amount big.
Content of the invention
It is an object of the invention to provide a kind of preparation method of ganglioside extract.
Another object of the present invention is to providing the ganglioside extract that described preparation method prepares.
For reaching above-mentioned purpose, on the one hand, the invention provides a kind of preparation method of ganglioside extract, described side
Method comprises the steps:
(1) take fresh Medulla sus domestica, tear meninges and vascular surface, then Medulla sus domestica is carried out microwave vacuum drying process, described
Microwave vacuum drying vacuum 0.04～0.09mpa, obtain water content be less than 5wt% dry Medulla sus domestica, by dry pig's brain powder
It is broken into pig's brain powder；
(2) add dehydrated alcohol in the pig's brain powder that step (1) obtains, stir 2.0 in temperature to 35～55 DEG C of conditions～
5.0 hours, through filtering, it is precipitated thing i；
(3) add in the precipitate i of step (2) NaAc_HAc buffer solution of ph5.0～5.4, isopropanol and
Dichloromethane mixed solvent, stirs 60～120min at ambient temperature, by centrifugation, obtains supernatant i；
(4) supernatant i being cooled to -15～-25 DEG C has precipitate ii to separate out, and filters, is precipitated thing ii；
(5) the precipitate ii that step (4) is obtained absolute ether cyclic washing twice more than, through filtering, vacuum drying,
Obtain ganglioside extract.
In a certain embodiment, the described vacuum degree control 0.05～0.07mpa of microwave vacuum drying, temperature control exist
40～55 DEG C.
In the preparation method of the ganglioside that the present invention provides, before anhydrous ethanol solvent is processed, first to new
Fresh Medulla sus domestica carries out microwave vacuum process.Microwave is the electromagnetic wave that a kind of wavelength is 1.0mm～1.0m, produces electromagnetic field of high frequency,
Under this electromagnetic field effect, the polar water molecules in Medulla sus domestica are arranged according to the polarity of electric field from original random distribution state shift
Row orientation, under electromagnetic field effect, the motion of formation molecule and phase mutual friction are thus produce energy so that medium temperature constantly carries
Height is so as to rapid heat, be dried.By microwave-vacuum drying vacuum, heating-up temperature control so that Medulla sus domestica plus
Thermal velocity is fast, and drying efficiency is high, and uniformly, dry mass is high for conduction of heat.And it is very beneficial for follow-up pulverizing, it is dry that pulverizing obtains
Pig's brain powder particle size distribution is narrow.
In the present invention, through microwave vacuum drying Medulla sus domestica moisture control in wt below 5%, by microwave vacuum
Dried, Medulla sus domestica can produce certain expansion effect.On the one hand it is easy to pulverize, on the other hand, there is the Medulla sus domestica of expansion effect
After powder is mixed with solvent, pig's brain powder is more beneficial for being fully contacted with solvent, more effectively removes non-targeted material.
In step (1), the Task-size Controlling of the pig's brain powder that dry Medulla sus domestica is ground into is in 80～150nm.
In certain embodiments, in step (2), the consumption of dehydrated alcohol is 5～10 times of pig's brain powder weight.
In some embodiments, in step (2), speed of agitator turns/min for 300-500.
In the present invention, the strict moisture content controlling Medulla sus domestica in step (1), using dehydrated alcohol more effectively by pig
Other lipid materials in brain powder remove, but, all kinds of materials of ganglioside almost do not run off.In the present invention, more
Preferably, the consumption of dehydrated alcohol is 6～8 times of pig's brain powder weight.
In certain embodiments, in step (3), the NaAc_HAc buffer solution of ph5.0～5.4, isopropanol with
And the volume ratio of dichloromethane three is (4～6.5): (0.7～1.5): (3～5.5).
In certain embodiments, in step (3), the consumption of mixed solvent is 4～8 times of weight of precipitate.
In the present invention, the precipitate i processing through dehydrated alcohol is formed with buffer solution, isopropanol and dichloromethane
Mixed solvent, more effectively ganglioside is extracted.And the NaAc_HAc buffer solution in ph5.0～5.4,
And in mixed solvent, other ganglioside moieties are converted into Monostalotetrahexosylgangliside (gm1).It is also
A high major reason of Monostalotetrahexosylgangliside comparision contents in the whole total fat of ganglioside.
In certain embodiments, in step (3), the rate controlled of supernatant i cooling is in 5～10 DEG C/h.
In certain embodiments, in step (3), supernatant i is cooled to -15～-25 DEG C afterwards, keeping temperature -
15～-25 DEG C of state 2～3 hours.
On the other hand, present invention also offers the ganglioside extract for preparing of described preparation method, in nerve
In section glycosides fat extract, gm1Content be higher than more than 15wt%.
In sum, its preparation method of the ganglioside extract of the present invention has the advantage that
The preparation method of the present invention is relatively easy, and the use of hazardous solvent has been greatly reduced in preparation process, and makes
In the standby extract obtaining, ganglioside purity is high, gm1Content high.Therefore, the ganglioside extract of the present invention is no
By be as extract Monostalotetrahexosylgangliside (gm1) raw material or directly take, be all beneficial.
Specific embodiment
The further details of introduction of ganglioside preparation method to the present invention below, but it is not restricted to the present invention's
Protection domain, protection scope of the present invention is defined by claims.In the present invention, term " embodiment ", " some realities
Apply scheme ", the feature of the expression such as " another embodiment " all can be with other " embodiments ", " some embodiments ", " another
The feature of one embodiment " is combined.
In the present invention, right using document svennerholm.l.methods in engymology (vi) 453.1963
Embodiment and test example product carry out the mensure of ganglioside content.
Embodiment 1
(1) take fresh Medulla sus domestica, tear meninges and vascular surface, cleaned with water, the Medulla sus domestica after cleaning carries out microwave vacuum
Dried, in 0.05～0.06mpa, at 45～50 DEG C, power is temperature control the vacuum degree control of microwave vacuum drying
2kw, obtains the dry Medulla sus domestica that water content is 3.5wt%, dry Medulla sus domestica is ground into pig's brain powder, and the particle mean size of pig's brain powder is 80～
150nm；
(2) take 1kg pig's brain powder, the pig's brain powder obtaining adds the dehydrated alcohol of 5.6l, in 38～43 DEG C of conditions of temperature
Stirring 3.5 hours, speed of agitator is 300 turns/min, afterwards through filtering, is precipitated thing i；
(3) add NaAc_HAc buffer solution, isopropanol and the dichloromethane mixing of ph5.4 molten in precipitate i
Agent, the consumption of mixed solvent is 6 times of weight of precipitate, wherein, the body of buffer solution, isopropanol and dichloromethane three
Long-pending ratio is 6.5:1.2:5.5, stirs 100min at ambient temperature, by centrifugation, obtains supernatant i；
(4) supernatant i is cooled to -20 DEG C with speed for 5 DEG C/h, keeps 2 hours at -20 DEG C, have precipitate ii to separate out,
Filter, be precipitated thing ii；
(5) the precipitate ii obtaining uses absolute ether cyclic washing three times at ambient temperature, each absolute ether plus
Enter 3 times that amount is precipitate ii weight, through filtering, being vacuum dried, obtain ganglioside extract.Through hplc nh 2 column method pair
Ganglioside extract is measured, and the content of the total fat of ganglioside is 96.7%, wherein, gm1Content be 26.5%.
Embodiment 2
(1) take fresh Medulla sus domestica, tear meninges and vascular surface, cleaned with water, the Medulla sus domestica after cleaning carries out microwave vacuum
Dried, in 0.07～0.08mpa, at 45～50 DEG C, power is temperature control the vacuum degree control of microwave vacuum drying
2kw, obtains the dry Medulla sus domestica that water content is 2.9wt%, dry Medulla sus domestica is ground into pig's brain powder, and the particle mean size of pig's brain powder is 80～
150nm；
(2) take 1kg pig's brain powder, the pig's brain powder obtaining adds the dehydrated alcohol of 6.2l, in 45～50 DEG C of conditions of temperature
Stirring 3.5 hours, speed of agitator is 300 turns/min, afterwards through filtering, is precipitated thing i；
(3) add NaAc_HAc buffer solution, isopropanol and the dichloromethane mixing of ph5.0 molten in precipitate i
Agent, the consumption of mixed solvent is 6 times of weight of precipitate, wherein, the volume of buffer solution, isopropanol and dichloromethane three
Ratio for 4.5:0.8:3.0, stirs 100min at ambient temperature, by centrifugation, obtains supernatant i；
(4) supernatant i is cooled to -20 DEG C with speed for 10 DEG C/h, keeps 3 hours at -20 DEG C, have precipitate ii to analyse
Go out, filter, be precipitated thing ii；
(5) the precipitate ii obtaining uses absolute ether cyclic washing three times at ambient temperature, each absolute ether plus
Enter 3 times that amount is precipitate ii weight, through filtering, being vacuum dried, obtain ganglioside extract.Through hplc nh 2 column method pair
Ganglioside extract is measured, and the content of the total fat of ganglioside is 95.3%, wherein, gm1Content be 35.4%.
Embodiment 3
(1) take fresh Medulla sus domestica, tear meninges and vascular surface, cleaned with water, the Medulla sus domestica after cleaning carries out microwave vacuum
Dried, in 0.05～0.06mpa, temperature control, at 40～45 DEG C, obtains moisture content to the vacuum degree control of microwave vacuum drying
Content is the dry Medulla sus domestica of 4.0wt%, dry Medulla sus domestica is ground into pig's brain powder, the particle mean size of pig's brain powder is 80～150nm；
(2) take 1kg pig's brain powder, the pig's brain powder obtaining adds the dehydrated alcohol of 7.5l, in 35～40 DEG C of conditions of temperature
Stirring 4.5 hours, speed of agitator is 400 turns/min, afterwards through filtering, is precipitated thing i；
(3) add NaAc_HAc buffer solution, isopropanol and the dichloromethane mixing of ph5.0 molten in precipitate i
Agent, the consumption of mixed solvent is 5 times of weight of precipitate, wherein, the volume of buffer solution, isopropanol and dichloromethane three
Ratio for 5.5:1.0:3.5, stirs 60min at ambient temperature, by centrifugation, obtains supernatant i；
(4) supernatant i is cooled to -15 DEG C with speed for 8 DEG C/h, keeps 3 hours at -15 DEG C, have precipitate ii to separate out,
Filter, be precipitated thing ii；
(5) the precipitate ii obtaining uses absolute ether cyclic washing three times at ambient temperature, each absolute ether plus
Enter 3 times that amount is precipitate ii weight, through filtering, being vacuum dried, obtain ganglioside extract.Through hplc nh 2 column method pair
Ganglioside extract is measured, and the content of the total fat of ganglioside is 94.9%, wherein, gm1Content be 27.2%.
Comparative example 1
The technological process of this comparative example reference implementation example 1 and technological parameter, different, fresh pig in this comparative example
Brain does not carry out the step (1) of embodiment 1, does not carry out microwave vacuum drying and pulverizing.Instead mode is, fresh
Stir into pulpous state with pulverizer after Medulla sus domestica deionized water drip washing, add the acetone 200ml extraction being cooled to 4 DEG C in advance, filter, obtain
Filter cake carries out the other step of embodiment 1.Obtain ganglioside.Through hplc nh 2 column method, ganglioside extract is carried out
Measure, the content of the total fat of ganglioside is 82.4%, wherein, gm1Content be 10.2%.
Comparative example 2
The technological process of this comparative example reference implementation example 1 and technological parameter, different, to fresh in this comparative example
The buffer solution adopting in the step (3) of Medulla sus domestica embodiment 1 is without, in this comparative example, the di(2-ethylhexyl)phosphate of ph6.5 adopted by buffer solution
Hydrogen sodium-disodium hydrogen phosphate buffer solution.Obtain ganglioside.Through hplc nh 2 column method, ganglioside extract is surveyed
Fixed, the content of the total fat of ganglioside is 89.4%, wherein, gm1Content be 7.5%.
Claims (9)
1. a kind of preparation method of ganglioside extract is it is characterised in that methods described comprises the steps:
(1) take fresh Medulla sus domestica, tear meninges and vascular surface, then Medulla sus domestica is carried out microwave vacuum drying process, described is micro-
Ripple vacuum drying vacuum 0.04～0.09mpa, obtains the dry Medulla sus domestica that water content is less than 5wt%, dry Medulla sus domestica is ground into
Pig's brain powder；
(2) add dehydrated alcohol in the pig's brain powder that step (1) obtains, little to 35～55 DEG C of condition stirrings 2.0～5.0 in temperature
When, through filtering, it is precipitated thing i；
(3) NaAc_HAc buffer solution, isopropanol and the dichloro of ph5.0～5.4 are added in the precipitate i of step (2)
Methane blended solvent, stirs 60～120min at ambient temperature, by centrifugation, obtains supernatant i；
(4) supernatant i being cooled to -15～-25 DEG C has precipitate ii to separate out, and filters, is precipitated thing ii；
(5) the precipitate ii that step (4) is obtained absolute ether cyclic washing twice more than, through filtering, vacuum drying, obtain
Ganglioside extract.
2. preparation method according to claim 1 is it is characterised in that the vacuum degree control of described microwave vacuum drying
0.05～0.07mpa, temperature control are at 40～55 DEG C.
3. preparation method according to claim 1 is it is characterised in that in step (2), the consumption of dehydrated alcohol is Medulla sus domestica
5～10 times of grain weight amount.
4. preparation method according to claim 1 is it is characterised in that in step (3), buffer solution, isopropanol and
The volume ratio of dichloromethane three is (4～6.5): (0.7～1.5): (3～5.5).
5. the preparation method according to claim 1 or 4 is it is characterised in that in step (3), the consumption of mixed solvent is
4～8 times of weight of precipitate.
6. preparation method according to claim 1 is it is characterised in that in step (3), the speed control of supernatant i cooling
System is in 5～10 DEG C/h.
7. the preparation method according to claim 1 or 7 is it is characterised in that in step (3), supernatant i is cooled to -15
～-25 DEG C afterwards, in the state 2～3 hours of -15～-25 DEG C of keeping temperature.
8. preparation method according to claim 1 is it is characterised in that in step (1), the pig's brain powder that dry Medulla sus domestica is ground into
Task-size Controlling in 80～150nm.
9. preparation method according to claim 1 is it is characterised in that in step (2), and speed of agitator turns for 300-500/
min.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106905387A (en) * | 2017-03-20 | 2017-06-30 | 泸州瑞兴生物工程有限公司 | A kind of preparation method of GM1 |
| CN109464464A (en) * | 2018-09-06 | 2019-03-15 | 四川鼎润元生物科技有限公司 | A kind of functional oral liquid and preparation method thereof improving cranial nerve tissue |
| CN109705176A (en) * | 2019-01-23 | 2019-05-03 | 苏州纳微科技股份有限公司 | The isolation and purification method of one boar gangliosides |
| CN112076218A (en) * | 2020-09-30 | 2020-12-15 | 江苏恒新药业有限公司 | Preparation method of pig brain acetone powder |
| CN113416222A (en) * | 2021-07-01 | 2021-09-21 | 嘉必优生物技术（武汉）股份有限公司 | Sialic acid particles and preparation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2002155A1 (en) * | 1988-11-04 | 1990-05-04 | Kailash C. Gupta | Process for the isolation of purified gangliosides from ganglioside-containing living tissue |
| CN1535974A (en) * | 2003-04-04 | 2004-10-13 | 上海绿谷（集团）有限公司 | Method for producing glucolipid substance using ganglioside as main component |
| CN1799552A (en) * | 2005-01-06 | 2006-07-12 | 严家定 | Single sialic acid tetrahexose ganglioside preparation method |
| CN101003553A (en) * | 2007-01-18 | 2007-07-25 | 吴浩青 | Method for extracting ganglioside with biological activity from animal tissues |
| CN102093440A (en) * | 2010-12-28 | 2011-06-15 | 山东新时代药业有限公司 | Coproduction process of pig brain protein hydrolysate and monosialoganglioside |
| CN103087120A (en) * | 2013-02-26 | 2013-05-08 | 北京四环制药有限公司 | Preparation method and application of monosialotetrahexosylganglioside |
| CN105111253A (en) * | 2015-09-18 | 2015-12-02 | 重庆寰瑞生物技术有限公司 | Method for extracting and separating ganglioside |
-
2016
- 2016-08-29 CN CN201610744824.9A patent/CN106349303A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2002155A1 (en) * | 1988-11-04 | 1990-05-04 | Kailash C. Gupta | Process for the isolation of purified gangliosides from ganglioside-containing living tissue |
| CN1535974A (en) * | 2003-04-04 | 2004-10-13 | 上海绿谷（集团）有限公司 | Method for producing glucolipid substance using ganglioside as main component |
| CN1799552A (en) * | 2005-01-06 | 2006-07-12 | 严家定 | Single sialic acid tetrahexose ganglioside preparation method |
| CN101003553A (en) * | 2007-01-18 | 2007-07-25 | 吴浩青 | Method for extracting ganglioside with biological activity from animal tissues |
| CN102093440A (en) * | 2010-12-28 | 2011-06-15 | 山东新时代药业有限公司 | Coproduction process of pig brain protein hydrolysate and monosialoganglioside |
| CN103087120A (en) * | 2013-02-26 | 2013-05-08 | 北京四环制药有限公司 | Preparation method and application of monosialotetrahexosylganglioside |
| CN105111253A (en) * | 2015-09-18 | 2015-12-02 | 重庆寰瑞生物技术有限公司 | Method for extracting and separating ganglioside |
Non-Patent Citations (1)
| Title |
|---|
| 潘颖，等: "神经节昔脂制备中溶剂效率的研究", 《首都医院院学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106905387A (en) * | 2017-03-20 | 2017-06-30 | 泸州瑞兴生物工程有限公司 | A kind of preparation method of GM1 |
| CN109464464A (en) * | 2018-09-06 | 2019-03-15 | 四川鼎润元生物科技有限公司 | A kind of functional oral liquid and preparation method thereof improving cranial nerve tissue |
| CN109464464B (en) * | 2018-09-06 | 2021-10-12 | 四川鼎润元生物科技有限公司 | Functional oral liquid for improving cranial nerve tissues and preparation method thereof |
| CN109705176A (en) * | 2019-01-23 | 2019-05-03 | 苏州纳微科技股份有限公司 | The isolation and purification method of one boar gangliosides |
| CN112076218A (en) * | 2020-09-30 | 2020-12-15 | 江苏恒新药业有限公司 | Preparation method of pig brain acetone powder |
| CN113416222A (en) * | 2021-07-01 | 2021-09-21 | 嘉必优生物技术（武汉）股份有限公司 | Sialic acid particles and preparation method thereof |
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Application publication date: 20170125 |