CN106345220A - Ethidium bromide adsorbing device - Google Patents
Ethidium bromide adsorbing device Download PDFInfo
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- CN106345220A CN106345220A CN201510410459.3A CN201510410459A CN106345220A CN 106345220 A CN106345220 A CN 106345220A CN 201510410459 A CN201510410459 A CN 201510410459A CN 106345220 A CN106345220 A CN 106345220A
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- ethidium bromide
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Abstract
The invention relates to an ethidium bromide (EB) adsorbing device, and aims to provide a device capable of adsorbing EB during nucleic acid gel electrophoresis, reducing or avoiding EB contamination of laboratory air while increasing the utilization rate of agarose gel, ensuring the uniformity of test, helping to promote the development of biology, medicine, drug design and other fields, and providing guarantees for safe operation of experimental personnel. The EB adsorbing device provided by the invention comprises a condensing box and an adsorbing box, wherein the top and the bottom of the condensing box are opened; the bottom of the adsorbing box is opened, and a top cover is arranged at the top of the adsorbing box; the bottom of the adsorbing box is communicated with the top of the condensing box; the condensing box comprises a condensing layer; a water inlet is formed in one side of the condensing layer, and a water outlet is formed in the other side; a through hole penetrating through an upper surface and a lower surface is formed in the condensing layer; the adsorbing box comprises an adsorbing layer; activated carbon coated with a breathable film is placed in the adsorbing layer; a through hole communicated with the through hole of the condensing layer is formed in the adsorbing layer.
Description
Technical field
The present invention relates to adsorbent equipment, more particularly to a kind of ethidium bromide adsorbent equipment, coagulate for agarose
The absorption of ethidium bromide in glue preparation and nucleic acid gel electrophoresis process.
Background technology
The gel electrophoresis of dna and rna is always one of normal experiment in biology and field of medicaments no matter
It is the checking in plasmid extraction, the displaying of pcr result, or the early stage sample preparation of gene order-checking
Recruitment evaluation, nucleic acid gel electrophoresis is all to provide nucleic acid purity, concentration, clip size etc. for experimenter
The most direct, most efficient method of information.Ethidium bromide (ethidium bromide, eb) is nov nucleic acid
The conventional dyes of separate nucleic acid detection during swimming, nucleic acid samples are combined and after agarose gel electrophoresis with eb,
Excited with standard 302nm ultraviolet light transmission instrument and radiate orange red chrominance signal, ultraviolet excitation nucleic acid can be passed through
- eb sends fluorescence, and the gel imaging processing system with polaroid egative film or with ccd imaging head shoots to be seen
Examine and determine size and the purity of nucleic acid fragment.
Have that Color is good, sensitivity is high, property is steady in nucleic acid gel electrophoresis process using eb dyestuff
Fixed, easily storage, low price and easy and simple to handle the advantages of；This technology mainly has following defect: the eb to be
High causing toxicity material, has very strong mutagenesis, easily cause cell occur canceration and high volatility (60～
Can volatilize for more than 70 DEG C) it is difficult to degrade again, this requirement sets up special eb contaminated area using the laboratory of eb,
The experimenter simultaneously carrying out nucleic acid electrophoresis with development test carries out careful protection, otherwise will be to experiment people
The even whole laboratory of member produces harm
When laboratory carries out nucleic acid electrophoresis, although operating personnel's band gloves are operated, it is to avoid eb passes through
The danger of contact contamination human body, but, electrophoresis process evaporates aerial eb, there is no to examination at present
Test operating personnel's safeguard procedures and preventive means.
With the development of biological medicine technology, the ease for use for dna and rna gel electrophoresis and wide usage
Higher and higher requirement is proposed.In recent years, countries in the world all be devoted to study eb substitute possibility,
As with genefinder, syprogreen etc. is nontoxic, low toxicity Fluorescent nucleic acid stain to replace eb, but these dyestuffs
Significantly limit its due to there are the shortcomings such as sensitivity is low, development effect is poor, can not reuse
Application.Therefore, when current laboratory carries out the gel electrophoresis of dna and rna, most widely used nucleic acid
Dyestuff remains eb.
In consideration of it, researching and developing a kind of device of adsorbable ethidium bromide, to reduce or to purify eb volatilization contaminated air
And experimental enviroment, reduce eb and very necessary is seemed to the harm of experimenter.
Content of the invention
The technical problem to be solved in the present invention is to provide a kind of bromination that can adsorb in nucleic acid gel electrophoresis process
The device of second ingot, while reducing or prevent eb pollution Laboratory air, and can improve the profit of Ago-Gel
With rate it is ensured that the homogeneity of test, be conducive to promoting biology and medical science, the development in the field such as drug design,
Provide safeguard for experimenter's safety operation.
For reaching above-mentioned purpose, a kind of present invention ethidium bromide adsorbent equipment, described adsorbent equipment includes condensing box
And adsorption box, described condensation box upper and lower opening, described adsorption box bottom opening, top is provided with top cover, described
Adsorption box bottom is connected with described condensation box top, and described condensation box includes condensate layer, described condensate layer side
It is provided with inlet opening, opposite side is provided with apopore, described condensate layer is provided with the passage running through top and bottom；Institute
State adsorption box and include adsorption layer, in described adsorption layer, be placed with the activated carbon of ventilated membrane parcel, described adsorption layer
It is provided with the open-work communicating with described condensate layer passage.
Wherein said condensation box and described adsorption box are aluminum alloy material.
Wherein said condensate layer is additionally provided with some condensation heads, and described condensation head is cone, and its tip deviates from institute
State adsorption box.
Wherein each column condensation head and each column passage interval setting.
Wherein said condensation box and described adsorption box are equivalently-sized.
Wherein said condensation box two bottom sides are provided with electrophoresis tank fixed plate, and described electrophoresis tank fixed plate is provided with half
Circular fixation hole.
Ethidium bromide adsorbent equipment difference from prior art of the present invention is that the present invention achieves following technology
Effect:
Ethidium bromide adsorbent equipment of the present invention, using cheap acticarbon, solves in glue and electricity
Because eb volatilizees during swimming, the problem of test for contamination environment, during reducing gel electrophoresis assay, eb is to reality
Test the harm of personnel；Meanwhile, gel maker makes gel maker close because adsorbent equipment covers after glue on gel maker
Envelope, so it is avoided that under open environment glue make moisture evaporation, cause gel strength change it is ensured that test
Fidelity.
The invention will be further described below in conjunction with the accompanying drawings.
Brief description
Fig. 1 is the structural representation of Ago-Gel gel maker；
Fig. 2 is the structural representation of ethidium bromide adsorbent equipment of the present invention；
Fig. 3 is buckled on the use state figure on gel device for ethidium bromide adsorbent equipment of the present invention；
Fig. 4 is applied to structural representation during electrophoretic apparatus for ethidium bromide adsorbent equipment of the present invention.
Description of reference numerals: 1- agarose glue module；2- glue pallet；3- glue comb；4- gel maker side
Seat；5- condenses box；6- adsorption box；7- condenses head；8- passage；9- inlet opening；10- apopore；11- electrophoresis
Groove fixed plate；12- fixing hole；13- top cover.
Specific embodiment
Below in conjunction with drawings and Examples, the above-mentioned He other technical characteristic of the present invention and advantage are made more detailed
Thin explanation.
As shown in Fig. 2 a kind of present invention ethidium bromide adsorbent equipment, described adsorbent equipment includes condensing box 5
With adsorption box 6, condense box 5 upper and lower opening, adsorption box 6 bottom opening, top is provided with top cover 13, absorption
Box 6 bottom is connected with condensation box 5 top, and condensation box 5 includes a condensate layer, and described condensate layer side is provided with
Inlet opening 9, opposite side is provided with apopore 10, and described condensate layer is provided with the passage 8 running through top and bottom, inhales
Attached box 6 includes an adsorption layer, is placed with the activated carbon of ventilated membrane parcel, described adsorption layer in described adsorption layer
It is provided with the open-work communicating with described condensate layer passage.
Embodiment 1: adsorb the eb of volatilization in the preparation process of Ago-Gel
As shown in figure 1, Ago-Gel gel maker is by the square agarose glue mould of 6 60 × 60mm
Block 1 and gel maker side seat 4 form.In glue, with the certain density agarose of microwave-oven-heating preparation
Appropriate eb is added after thawing.Ago-Gel added with eb is poured into and installs gel pallet 2 and system in advance
In the gel maker of glue comb 3, then the condensation box 5 of Fig. 2 ethidium bromide adsorbent equipment is covered in gel maker Fig. 1
Upper (as shown in Figure 3).Ethidium bromide adsorbent equipment shown in Fig. 2 includes two box bodys.One is condensation box 5,
Including a condensate layer, another for adsorption box 6, including an adsorption layer, condense box and adsorption box be equal in magnitude
Cuboid box-packed thing (cooling box 5；Adsorption box 6 is an activated charcoal)；The box mouth of condensation box 5 can be tight
Close is buckled on gel maker (Fig. 1).Condensation box 5 and adsorption box 6 are all made with aluminum alloy materials, adsorption layer
It is respectively provided on two sides with inlet opening 9 and apopore 10, before glue, add 0 DEG C in the condensate layer of condensation box first
Cold water.Condensation 7 and the passage 8 of cone are additionally provided with condensate layer.During glue, hot glue is waved
It is water that a large amount of vapor sending make Water vapor condensation under condensate layer cold service, after most water is assembled
Water droplet can be condensed into around taper condensation 7 to instill in gel maker.Passage 8 on condensate layer runs through cold
Solidifying layer, reaches adsorption layer (adsorption box 6), makes the eb volatilizing reach adsorption layer by passage 8；Inhale
Attached layer is adjacent with condensate layer, is provided with the open-work communicating with passage 8, adsorption box adsorption layer built with saturating
The activated carbon of air film parcel.During glue, the eb of volatilization can reach absorption through the passage of condensate layer
Layer, adsorbed in the layer activated carbon absorbs.According to laboratory ambient temperature height, during glue, when
The vapor of volatilization is being condensed into water through the condensate layer layer that is not condensed, or after glue a couple of days, condensation effect becomes
Difference, when activated carbon gets wet, can arbitrarily change cold water and the absorption in the layer activated carbon of condensate layer, to ensure to wave
The eb sending as much as possible adsorbed.
The cold water of condensate layer and absorption in the layer activated carbon can arbitrarily be changed.
Embodiment 2: adsorb the eb of volatilization during nucleic acid electrophoresis
Fig. 4 is used for schematic diagram, its mounting structure and the absorption of nucleic acid electrophoresis process for ethidium bromide adsorbent equipment
Principle is identical with Ago-Gel, and condensation box 5 lower casing mouth size is identical with electrophoresis tank size, lid mouth plane
Setting fit with electrophoresis tank, and its laminating plane be provided with electrophoresis tank fixed plate 11 and with electrophoresis tank positive and negative electrode
Fixing semicircle fixing hole 12.After electrophoresis sample-adding terminates, ethidium bromide adsorbent equipment (Fig. 4) is detained
On electrophoresis tank, fixing plate hole 12 is made to be buckled on positive and negative electrode.Ethidium bromide adsorbent equipment thus can be made
Be simply secured on electrophoresis tank, airtight adsorption box just can by the water vapor condensation volatilizing in electrophoresis process,
The pernicious gases such as the eb of volatilization are adsorbed the charcoal absorption of layer.According to laboratory ambient temperature height, in electricity
During swimming, when the vapor of volatilization is being condensed into water through the condensate layer layer that is not condensed, or electrophoresis is for several times
Condensation effect is deteriorated afterwards, when activated carbon gets wet, can arbitrarily change cold water and the absorption in the layer activity of condensate layer
Charcoal, to ensure the eb that volatilizes as much as possible adsorbed.
Embodiment described above is only the preferred embodiment of the present invention to be described, not to this
Bright scope is defined, on the premise of without departing from design spirit of the present invention, those of ordinary skill in the art
The various modifications that technical scheme is made and improvement, all should fall into claims of the present invention and determine
Protection domain in.
Claims (6)
1. a kind of ethidium bromide adsorbent equipment it is characterised in that: described adsorbent equipment includes condensing box and absorption
Box, described condensation box upper and lower opening, described adsorption box bottom opening, top is provided with top cover, described adsorption box
Bottom with described condensation box top connect, described condensation box include condensate layer, described condensate layer side be provided with into
Water hole, opposite side is provided with apopore, and described condensate layer is provided with the passage running through top and bottom；Described absorption
Box includes adsorption layer, be placed with described adsorption layer ventilated membrane parcel activated carbon, described adsorption layer be provided with
The open-work that described condensate layer passage communicates.
2. ethidium bromide adsorbent equipment according to claim 1 it is characterised in that: described condensation box and
Described adsorption box is aluminum alloy material.
3. ethidium bromide adsorbent equipment according to claim 1 it is characterised in that: described condensate layer is also
It is provided with some condensation heads, described condensation head is cone, and its tip deviates from described adsorption box.
4. ethidium bromide adsorbent equipment according to claim 3 it is characterised in that: each column condensation head with
Each column passage interval setting.
5. ethidium bromide adsorbent equipment according to claim 1 it is characterised in that: described condensation box and
Described adsorption box is equivalently-sized.
6. ethidium bromide adsorbent equipment according to claim 1 it is characterised in that: described condensation cassette bottom
Portion both sides are provided with electrophoresis tank fixed plate, and described electrophoresis tank fixed plate is provided with semicircle fixing hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510410459.3A CN106345220B (en) | 2015-07-13 | 2015-07-13 | Application of adsorption device in adsorption of volatilized ethidium bromide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510410459.3A CN106345220B (en) | 2015-07-13 | 2015-07-13 | Application of adsorption device in adsorption of volatilized ethidium bromide |
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| CN106345220A true CN106345220A (en) | 2017-01-25 |
| CN106345220B CN106345220B (en) | 2023-04-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510410459.3A Active CN106345220B (en) | 2015-07-13 | 2015-07-13 | Application of adsorption device in adsorption of volatilized ethidium bromide |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5582702A (en) * | 1995-04-26 | 1996-12-10 | Ethrog Biotechnology Ltd. | Apparatus and method for electrophoresis |
| US20050202970A1 (en) * | 2003-08-15 | 2005-09-15 | Taiwan Proteomics Co., Ltd. | Agent and a method for removing ethidium bromide in a waste solution |
| JP2006000700A (en) * | 2004-06-15 | 2006-01-05 | Canon Inc | Structure for adsorbing dna intercalation substance |
| CN2755600Y (en) * | 2004-09-20 | 2006-02-01 | 林�源 | Separated ethyene-spindle bromide treater with running gel dye |
| CN201249066Y (en) * | 2008-06-11 | 2009-06-03 | 李恒 | Miniature filtering device for eliminating pollution from ethidium bromide in waste solution of libratory efficiently |
| CN101570785A (en) * | 2009-06-10 | 2009-11-04 | 南京大学 | Method for detecting potential inherent toxicity of organic pollutants in water body |
| CN103760137A (en) * | 2014-01-10 | 2014-04-30 | 厦门大学 | Cell DNA damage detection method |
| CN204789459U (en) * | 2015-07-13 | 2015-11-18 | 中国农业科学院兰州兽医研究所 | Ethidium bromide adsorption equipment |
-
2015
- 2015-07-13 CN CN201510410459.3A patent/CN106345220B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5582702A (en) * | 1995-04-26 | 1996-12-10 | Ethrog Biotechnology Ltd. | Apparatus and method for electrophoresis |
| US20050202970A1 (en) * | 2003-08-15 | 2005-09-15 | Taiwan Proteomics Co., Ltd. | Agent and a method for removing ethidium bromide in a waste solution |
| JP2006000700A (en) * | 2004-06-15 | 2006-01-05 | Canon Inc | Structure for adsorbing dna intercalation substance |
| CN2755600Y (en) * | 2004-09-20 | 2006-02-01 | 林�源 | Separated ethyene-spindle bromide treater with running gel dye |
| CN201249066Y (en) * | 2008-06-11 | 2009-06-03 | 李恒 | Miniature filtering device for eliminating pollution from ethidium bromide in waste solution of libratory efficiently |
| CN101570785A (en) * | 2009-06-10 | 2009-11-04 | 南京大学 | Method for detecting potential inherent toxicity of organic pollutants in water body |
| CN103760137A (en) * | 2014-01-10 | 2014-04-30 | 厦门大学 | Cell DNA damage detection method |
| CN204789459U (en) * | 2015-07-13 | 2015-11-18 | 中国农业科学院兰州兽医研究所 | Ethidium bromide adsorption equipment |
Non-Patent Citations (2)
| Title |
|---|
| ALI FAKHRI: "Assessment of Ethidium bromide and Ethidium monoazide bromide removal from aqueous matrices by adsorption on cupric oxide nanoparticles", 《ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY》 * |
| 王丽敏 等: "活性炭搭载TiO2降解溴化乙锭的试验研究", 《哈尔滨师范大学自然科学学报》 * |
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| CN106345220B (en) | 2023-04-28 |
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