CN106338476A - Glutathione reductase activity unit conversion method - Google Patents
Glutathione reductase activity unit conversion method Download PDFInfo
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- CN106338476A CN106338476A CN201610676389.0A CN201610676389A CN106338476A CN 106338476 A CN106338476 A CN 106338476A CN 201610676389 A CN201610676389 A CN 201610676389A CN 106338476 A CN106338476 A CN 106338476A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
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Abstract
A glutathione reductase activity unit conversion method is disclosed. GR activity unit expressed by illegal unit such as various units which do not conform to our national legal unit of measurement and non-SI unit is converted to SI unit such that quantity and unit of articles in a journal conform more to our national laws related to quantity and unit. Conversion is respectively carried out according to different GR measurement methods. Thus, accuracy of conversion is guaranteed, and conversion efficiency is raised.
Description
Technical field
The invention belongs to agricultural medical science and publishing area, the international standard control method of especially a kind of enzyme-activity unit,
Specifically a kind of glutathione reductase activity unit conversion method.
Background technology
The International System of Units (international system of units) is that General Conference of Weights and Measures (cgpm) is adopted and pushed away
A kind of coherent system of units recommended, passes through in the 11st General Conference of Weights and Measures of nineteen sixty it is recommended that various countries adopt, referred to as si.
On September 6th, 1985, the Standing Committee of the National People's Congress formulates and has passed through " Measurement Law of the People's Republic of China ".This regulation is clearly declared
Cloth: " country adopts the International System of Units.International System of Units measurement unit and national other measurement units selected, legal for country
Measurement unit.Non- national legal unit of measurement should abolish." and also to the needs of academic exchange are it should use si unit.
International Union of Biochemistry in 1964 recommends to be determined according to the report of EC of International Union of Biochemistry in 1961
(unit, referred to as u), the enzyme-activity unit of a standard is defined as in the optimum reaction conditionses determining one standard enzyme-activity unit of justice
Under, the enzyme amount being catalyzed required for 1 μm of ol substrate per minute, it is defined as 1u, here it is conventional unit of enzyme activity.But u is not
It is the base unit of the International System of Units (si), is not " coherent derived unit ", therefore, in order to follow wanting of si
Ask, 1972, biochemical nomenclature commission's suggestion catalysis in " enzyme nomenctature, recommendations "
Reaction rate should be expressed as the form of mol/s, simultaneously in this this book, according to carrying of (systeme international)
View proposes and defines a new enzyme-activity unit (katal, abbreviation kat), for enzyme list before replacing, not named
Position, after this kind of definition, but still has a lot of periodicals to employ u as the active unit of enzyme.
In the existing gb3100-1993 of China " International System of Units and its application " and gb3101-1993 " relevant amount, unit
With the rule meeting " although and also clearly not providing the unit of enzyme activity in gb3102, in gb3100-1993
Give the composition of the International System of Units, and propose that " all belong to the legal metering list of unit Dou Shi China of the International System of Units
Position ", points out " according to " indivedual science in " suggestion of China's legal unit of measurement of carrying out in an all-round way " in this two standards simultaneously again
In technical field, if any the special requirement legal unit of measurement, but the title that also must specify with relevant international organization, symbol phase one
The principle causing ", but there is chemical reaction conversion rate in " amount of physical chemistry and molecular physics and the unit " of gb3102.8
Si unit be " mol/s " definition, although in the GB of China not directly define enzyme active unit, the work of enzyme
Property be to be embodied by the reactant of chemical reaction is converted into product, so the active unit of enzyme should with reaction conversion speed
The unit of rate is consistent, and its legal unit of enzyme activity is per second for rubbing, and symbol is mol/s, and the enzyme-activity unit meeting the requirement of si unit is
Kat, conventional multiple unit is nkat or μ kat, this just definition phase one to enzyme unit with biochemical nomenclature commission
Cause, that is, the implication of enzyme is under optimum reaction conditionses, be catalyzed the enzyme amount required for the change of 1mol substrate each second, this is a kind of logical
Use method for expressing.
At present, the present situation of domestic and international periodical GSH-PX activity reductase activity unit is as follows:
Glutathione reductase (glutathione reductase, gr) is that one kind will be aoxidized using reduced form nad (p)
Type glutathione (gs-sg) catalytic reaction becomes the enzyme ec.1.6.4.2 of reduced form (gsh).The height of gr activity is often as raw
The index of the state of oxidation in object, is therefore widely used in the article of agricultural doctor and Journal of Biology Science.For example using paddy
The sweet fabk polypeptide of Guang set as the academic total storehouse (http://scholar.cnki.net/) of descriptor search Hownet, time of publishing
For 2015, have 231 documents and contain glutathione reductase, wherein english article 114, Chinese core 91, be related to 40
Individual ambit (according to Subject division in Hownet), this explanation gr is a relatively common enzyme in technical paper, has wide
General application, but there are the various forms of expression in the active unit of gr, employ si unit, and some use in some articles
The method for expressing of non-si unit.
It is antioxidant enzymes in springer database using " with all of the words "
Carry out Advanced Search, set and be limited in year 2013 to 2014 years, download and maximally related front 45 articles of search keyword, cover 24
Plant magazine.Wherein it is used gr to have 21 articles as index, μm ol/min or mmol/min is wherein used as enzyme activity list
Position have 11, be used u to have 8 as enzyme-activity unit, have 11/m to have 1 as its enzyme-activity unit using μ, picture does not occur
Cat with pod is the same to be used od value as the article of active unit.Glutathione reductase is used as in descriptor search simultaneously
National IP Network's database, condition is set as from core periodical, is published in 2015, and languages are Chinese, and ambit is crops
Learn (21), biology (13), forestry (4) and plant protection subject (3), totally 39 articles, wherein measure gluathione
The article of fabk polypeptide activity has 28, is used u to have 10 as unit, and nmol/min and μm ol/min has 11,
6 of u/min, have 1 to use △ a340/ min is as its gr unit of enzyme activity.
Reactant according to gr catalytic reaction and product measure the activity of gr typically by several method: the 1) reaction of gr catalysis
Thing nadph is due to being oxidized to nadp+, the absorbance under 340nm can reduce, with unit interval nadph under 340nm extinction
Angle value reduces speed to represent the activity of gr, and this method is all used in a lot of documents, is a kind of side of the mensure gr of standard
Method, simultaneously because gr is to be catalyzed nadph and gssg to generate gsh and nadp+, in this reaction 1mol nadph oxidation
Meanwhile, gssg is also the reduction of 1mol, so, if in some papers of delivering, define gssh as the definition of gr enzyme and
Using nadph as the definition of gr enzymatic activity be numerically identical, so here generally with nadph define gr activity for mark
Accurate；2) activity of gr is passed through to measure reactant liquor in interpolation GSSG (gssg) and dtnb (5,5 dithiobis 2
Nitrobenzoic acid), under 412nm, representing, this method is to gr activity for the speed that product tnb absorbance increases
Indicate two types, a kind of is △ a412/ min or u, molar absorption coefficient that can be according to product tnb under 412nm
Being converted, another kind does calibration curve with the gr standard items of sigma company and carries out enzymatic activity 14.15l/ (mmol × cm)
Compare, this method for expressing, due to having standard items as reference, can only be converted by researcher.
Content of the invention
The purpose of the present invention is the conversion problem for glutathione reductase enzyme-activity unit, proposes a kind of glutathione also
Original enzyme activity unit conversion method.
The technical scheme is that
A kind of glutathione reductase activity unit conversion method, it comprises the following steps:
S1, the assay method of acquisition glutathione reductase gr, when this assay method is by measuring reactant nadph
When the change of (NADPH) absorbance is to represent gr enzyme activity, extract defined in the method
Amount x of absorbance change per minute and enzyme activity amount y of gr to be converted, unit is u；
S2, adopt following formula that enzyme activity amount y of gr is scaled the international standard list with kat (mol/s) as representation
Position si unit processed:
Wherein x is the fundamental unit that glutathione reductase is gr activity, for defining absorbance change per minute
Amount, y is the amount of gr enzyme activity to be converted, and z is the amount of the gr enzyme activity being represented using kat (mol/s), and 60 is that minute is scaled the second
Conversion coefficient, 1000 is that the molar absorption coefficients of nadph are converted to the conversion coefficient of mol by mmol, and 6.22 exist for nadph
Molar absorption coefficient under 340nm, unit is: l/ (mmol cm).
In the present invention, by measure reactant nadph absorbance change represent gr enzyme activity method particularly as follows:
Under the conditions of to be measured, during 340nm wavelength, the unit that definition absorbance a (340) per minute changes is x as 1 gr active unit
The activity of u, the gr measuring in this way is y, and unit is u.
A kind of glutathione reductase activity unit conversion method, it comprises the following steps:
S1, the assay method of acquisition glutathione reductase gr, when this assay method is that absorbance conduct is directly used
During the enzyme activity amount of gr, extract enzyme activity amount y of gr to be converted in the method, unit is: △ a340/min；
S2, adopt following formula that enzyme activity amount y of gr is scaled the international standard list with kat (mol/s) as representation
Position si unit processed:
Wherein y is the amount of gr enzyme activity to be converted, using △ a340/ min represents, z is the gr being represented using kat (mol/s)
The amount of enzyme activity, 60 is the conversion coefficient that minute is scaled the second, and 1000 molar absorption coefficients for nadph are converted to by mmol
The conversion coefficient of mol, 6.22 is molar absorption coefficient under 340nm for the nadph, and unit is: l/ (mmol cm).
A kind of glutathione reductase activity unit conversion method, it comprises the following steps:
S1, the assay method of acquisition glutathione reductase gr, when this assay method is by measuring product tnb (nitro
Benzoic acid) absorbance change to represent gr enzyme activity when, extract enzyme activity amount y of gr to be converted in the method, unit is: △
a412/min；
S2, adopt following formula that enzyme activity amount y of gr is scaled the international unit with kat (mol/s) as representation:
Wherein y is the amount of gr enzyme activity to be converted, and unit is: △ a412/ min, z are the gr being represented using kat (mol/s)
The amount of enzyme activity, 60 is the conversion coefficient that minute is scaled the second, and 1000 molar absorption coefficients for tnb are converted to mol by mmol
Conversion coefficient, 14.15 be molar absorption coefficient under 412nm for the tnb, unit is: l/ (mmol cm).
Beneficial effects of the present invention:
The present invention does not meet, by various, the gr activity list that the illegal unit such as China's legal unit of measurement and non-si unit represents
Position is scaled si unit, makes the use of article amount and unit in periodical more meet the relevant laws with regard to amount and unit for the China.
The present invention is converted respectively according to the different methods measuring gr, and will be soft to a computer for this kind of conversion set
In part, this conversion process is completed by computer it is ensured that the accuracy of conversion, improve the efficiency of conversion simultaneously.
Brief description
Fig. 1 is glutathione reductase (gr) si unit conversion interface.
Fig. 2 declines interface (the definition unit interval absorbance to represent gr activity to measure reactant nadph absorbance
Value changes are 1u)
Fig. 3 to represent the interface of gr activity to measure tnb absorbance and to raise
Fig. 4 selects gr activity by the prompting interface obtaining with gr standard items curve comparison
Fig. 5 is under 340nm, represents that gr activity (is represented with absorbance change, △ a with nadph340/ min) interface
Specific embodiment
With reference to embodiment, the present invention is further illustrated.
The interface of the present invention such as Fig. 1, selects glutathione reductase (gr) reductase in Fig. 1 " please select assay method "
Method, the method according to the method for the activity of commonly used mensure gr at present, user according to deliver the explanation of content part Lai
Carry out selecting different methods to be converted.
1) when select " under 340nm, with nadph represent gr activity (with light absorption value define enzyme-activity unit, u) ", occur Fig. 2's
Interface, and activate conversion button, in " enzyme-activity unit definition please be input " text below frame of Fig. 2, the basis of input gr
The enzyme-activity unit of definition, absorbance generally per minute declines how much to be defined as an enzyme-activity unit, is simultaneously entered basis
This kind of assay method total enzyme activity amount to be converted.For example, define under certain condition here, under 340nm wavelength, between with 30s being
Every scanning 3min., a (340) change 0.1 per minute is 125u as 1 gr active unit (u), gr enzyme activity total amount to be converted,
Converted according to formula (1), the gr activity total amount being represented for unit using kat is 3.349 × 10-5Mol/s (is counted with science
Method 3.349e-5 represents).
2) when selecting, " under 412nm, in the unit interval, product tnb represents that (unit is △ a to gr activity412/ min) " when, occur
The interface of Fig. 3, activation conversion button, in " please input total enzyme amount to be converted " text below frame of Fig. 3, input will convert
Total enzyme activity amount, according to introduced in content of the invention, with △ a412/ min to represent as unit, here due to not having
Define total base unit of enzyme, so directly being converted according to formula (3).For example, gr enzyme activity amount to be converted is 100 △
a412/ min, then scaling results are 1.178 × 10-4Mol/s (is represented with scientific notation 1.178e-4).
3) when selecting " activity of gr compares acquisition by the calibration curve with gr standard items ", the interface of Fig. 4 occurs, changes
Calculate button unavailable, occur " please directly converting (researcher can be contacted) according to standard items curve！" printed words, so remind change
Calculation person carries out follow-up operation.
4) when with nadph, selection " under 340nm, represents that gr activity (is represented with absorbance, △ a340/ min) " when, occur
The interface of Fig. 5, activation conversion button, in " please input total enzyme amount to be converted " text below frame of Fig. 5, input will convert
Total enzyme activity amount, total enzyme activity amount to be converted here, according to introduced in content of the invention, is with △ a340/ min is as list
Position representing, here due to not defining total base unit of enzyme, so directly being converted according to formula (2).For example,
The gr enzyme activity amount of conversion is 100 △ a340/ min, then scaling results are 2.680 × 10-4Mol/s is (with scientific notation
2.680e-4 representing).
Part that the present invention does not relate to is all same as the prior art or can be realized using prior art.
Claims (4)
1. a kind of glutathione reductase activity unit conversion method, is characterized in that it comprises the following steps:
S1, the assay method of acquisition glutathione reductase gr, when this assay method is by measuring reactant reduced form nicotinoyl
When the change of amine adenine-dinucleotide phosphoric acid nadph absorbance is to represent gr enzyme activity, extract per minute defined in the method
Amount x of absorbance change and enzyme activity amount y of gr to be converted, unit is u；
S2, adopt following formula that enzyme activity amount y of gr is scaled the system international with kat (mol/s) as representation
Si unit:
Wherein x is the fundamental unit that glutathione reductase is gr activity, for defining the amount of absorbance change per minute, y
For the amount of gr enzyme activity to be converted, z is the amount of the gr enzyme activity being represented using kat (mol/s), and 60 is that minute is scaled changing of second
Calculate coefficient, 1 000 molar absorption coefficients for nadph are converted to the conversion coefficient of mol by mmol, 6.22 is nadph in 340nm
Under molar absorption coefficient, unit is: l/ (mmol cm).
2. glutathione reductase activity unit conversion method according to claim 1, is characterized in that reacting by mensure
The method to represent gr enzyme activity for the change of thing nadph absorbance is particularly as follows: under the conditions of to be measured, during 340nm wavelength, definition is every
The unit that minute absorbance a (340) changes is x as 1 gr active unit u, the activity of the gr measuring in this way
For y, unit is u.
3. a kind of glutathione reductase activity unit conversion method, is characterized in that it comprises the following steps:
S1, the assay method of acquisition glutathione reductase gr, when this assay method is absorbance to be directly used as gr's
During enzyme activity amount, extract enzyme activity amount y of gr to be converted in the method, unit is: △ a340/min；
S2, adopt following formula that enzyme activity amount y of gr is scaled the system international with kat (mol/s) as representation
(si) unit:
Wherein y is the amount of gr enzyme activity to be converted, using △ a340/ min represents, z is the gr enzyme activity being represented using kat (mol/s)
Amount, 60 is the conversion coefficient that minute is scaled the second, 1 000 be nadph molar absorption coefficients be converted to mol's by mmol
Conversion coefficient, 6.22 is molar absorption coefficient under 340nm for the nadph, and unit is: l/ (mmol cm).
4. a kind of glutathione reductase activity unit conversion method, is characterized in that it comprises the following steps:
S1, the assay method of acquisition glutathione reductase gr, when this assay method is by measuring product tnb (nitrobenzoyl
Acid) absorbance change to represent gr enzyme activity when, extract enzyme activity amount y of gr to be converted in the method, unit is: △ a412/
min；
S2, adopt following formula that enzyme activity amount y of gr is scaled the international unit with kat (mol/s) as representation:
Wherein y is the amount of gr enzyme activity to be converted, and unit is: △ a412/ min, z are the gr enzyme activity being represented using kat (mol/s)
Amount, 60 is the conversion coefficient that minute is scaled the second, 1 000 be tnb molar absorption coefficients changing of mol is converted to by mmol
Calculate coefficient, 14.15 is molar absorption coefficient under 412nm for the tnb, and unit is: l/ (mmol cm).
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107101866A (en) * | 2017-05-23 | 2017-08-29 | 苏州普瑞斯生物科技有限公司 | A kind of glutathione reductase determines reagent and its preparation method and application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1392901A (en) * | 2001-06-20 | 2003-01-22 | 朴英美 | Fast quantitatively measuring oxided and reduced glutahione by enzyme method |
| WO2003052390A1 (en) * | 2001-12-17 | 2003-06-26 | Reclos J George | Simultaneous analysis of blood samples and estimation of blood (hemoglobin) content |
| CN101390082A (en) * | 2006-02-27 | 2009-03-18 | E.P实验有限公司 | Method for optimization of measurement standard and industrial engineering calculation method using the same |
-
2016
- 2016-08-16 CN CN201610676389.0A patent/CN106338476A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1392901A (en) * | 2001-06-20 | 2003-01-22 | 朴英美 | Fast quantitatively measuring oxided and reduced glutahione by enzyme method |
| WO2003052390A1 (en) * | 2001-12-17 | 2003-06-26 | Reclos J George | Simultaneous analysis of blood samples and estimation of blood (hemoglobin) content |
| CN101390082A (en) * | 2006-02-27 | 2009-03-18 | E.P实验有限公司 | Method for optimization of measurement standard and industrial engineering calculation method using the same |
Non-Patent Citations (3)
| Title |
|---|
| A.斯泰因比歇尔: "《生物高分子 第6卷 多糖II：真核生物多糖》", 31 August 2004 * |
| 刘昌来等: "CAT、POD、APX 3种酶活力SI单位换算", 《农业图书情报学刊》 * |
| 王丽芳等: "几种自定义酶活性单位常见错误辨析及规范使用建议", 《中国科技期刊研究》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107101866A (en) * | 2017-05-23 | 2017-08-29 | 苏州普瑞斯生物科技有限公司 | A kind of glutathione reductase determines reagent and its preparation method and application |
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Application publication date: 20170118 |