CN106333969A - Agaricus gennadii HDAC1 enzyme inhibition effective part and preparation method and application - Google Patents
Agaricus gennadii HDAC1 enzyme inhibition effective part and preparation method and application Download PDFInfo
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- CN106333969A CN106333969A CN201610761356.6A CN201610761356A CN106333969A CN 106333969 A CN106333969 A CN 106333969A CN 201610761356 A CN201610761356 A CN 201610761356A CN 106333969 A CN106333969 A CN 106333969A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
The invention provides an agaricus gennadii HDAC1 enzyme inhibition effective part and a preparation method and application and belongs to the technical field of plant extracts. The effective part is selected from a mixture of one or more of an agaricus gennadii petroleum ether part, an agaricus gennadii ethyl acetate part, an agaricus gennadii n-butyl alcohol part and an agaricus gennadii aqueous phase extraction part according to any mass ratio. Agaricus gennadii is smashed, an ethyl alcohol solution with the volume percentage concentration being 65-95% is added for reflux extraction, and an obtained extracting solution is concentrated and dried to obtain an extract; the extract is added into distilled water to be dispersed, an extract dispersion solution is obtained and extracted by sequentially using petroleum ether, ethyl acetate and n-butyl alcohol as solvents, and the agaricus gennadii petroleum ether part, the agaricus gennadii ethyl acetate part and the agaricus gennadii n-butyl alcohol part are obtained after the solvents are removed through volatilization; the remaining extract dispersion solution obtained after extraction is concentrated and dried, and the agaricus gennadii aqueous phase extraction part is obtained. The agaricus gennadii HDAC1 enzyme inhibition effective part is applied to preparing HDAC1 enzyme inhibition medicine and can be used for preparing anti-tumor medicine.
Description
Technical field
The present invention relates to circle spore mushroom suppression hdac1 enzyme effective site and preparation method and application, belong to plant extract technology
Field.
Background technology
Circle spore mushroom agaricus gennadii belongs to Agaricaless Agaricus edibilis Agaricuses (agaricus), another name " circle spore support handle
Mushroom " [referring to: Yang Hui, Li Na. the investigation [j] of circle spore mushroom. the Nature, 1996(6): 48.].Circle spore mushroom is in umbrella shape, has
Strong mushroom fragrance.Milky during lamella children, after fade to pink to pitchy, single heavy 58 ~ 350g, maximum up to 2 ~
5kg.Summer and autumn is grown on shrubbery sand ground, in the soil of 20 ~ 70cm under lakeside phragmites communiss clump, underground can parachute-opening, Dan Sheng, scattered or clump
Raw, be grown in more western Xinjiang and Localities In Southwest [referring to Wang Junyan. Xinjiang edible and medical fungi resource and DEVELOPMENT PROSPECT [j]. micro-
Biology is circulated a notice of, 1997(3): 176-177.].
Malignant tumor is one of major disease of serious harm human health, it has also become the lethal first cause of the mankind.Dislike
The preventing and treating of property cancerous cell has become medical circle important topic of interest.But, Incidence is hidden, and the cause of disease is complicated, disease
Reason change is various, and it is treated with multiformity and the repeatability still being limited to Cancerous disease.Although the carcinogen of different cancers because
May be not quite similar, but its structure observation all shows similar cell cycling disorder and uncontrollable cell growth rapidly
And transfer.Therefore, to participate in cell cycle regulating some family proteins research promote treatment of cancer in become important because
Element.Histon deacetylase (HDAC) (hdac) is an albuminoid enzyme, and the structural modification and gene expression regulation of chromosome is played
Important effect.Generally, the acetylation of histone is conducive to the dissociation of dna and histone octamer, nucleosomal structure
Lax so that various transcription factor and collaborative transcription factor can specifically binds with dna binding site, activated gene turn
Record.In cancerous cell, the overexpression of hdac leads to the enhancing of deacetylation effect, by recovering histone positive charge, thus
Increase the gravitation between dna and histone, make lax nucleosome become very tight, be unfavorable for the expression of specific gene, bag
Include some tumor suppressor genes.Antibiotic FR 901228 (histone deacetylase inhibitors,
Hdaci) then can be by improving chromatin specific region acetylation of histone, thus regulating cell apoptosis and differentiation associated protein
Expression and stability, inducing cell apoptosis and differentiation, become the new antitumor drug of a class.Hdaci is not only to multiple blood systems
System tumor and solid tumor have good therapeutical effect, and have the advantages that the of a relatively high selectivity of tumor cell and low toxicity.
Applicant finds under study for action: the cost of material of antitumor drug is high at present, especially for preparation suppression hdac1
The raw material of Chinese medicine of enzyme medicine is more deficient.In prior art, circle spore mushroom only has edibility because of its delicious flavour.Not yet
See with regard to circle spore mushroom suppress hdac1 enzyme active in terms of research and application.
Content of the invention
The technical problem to be solved in the present invention is: overcomes the deficiencies in the prior art, provides round spore mushroom suppression hdac1 enzyme to have
Effect position and preparation method and application, this circle spore mushroom suppresses hdac1 enzyme effective site can effectively suppress the activity of hdac1 enzyme, is used for
Preparation suppression hdac1 enzyme medicine, has preferable antitumor action.
The technical solution adopted for the present invention to solve the technical problems is: this circle spore mushroom suppression hdac1 enzyme effective site,
It is characterized in that: selected from circle spore mushroom petroleum ether part, circle spore mushroom ethyl acetate extract, circle spore mushroom n-butanol portion, circle
One of spore mushroom aqueous phase extraction position or the two or more mixture by any mass ratio.
This circle spore mushroom suppression hdac1 enzyme effective site it is characterised in that: selected from circle spore mushroom n-butanol portion with circle spore
Mushroom petroleum ether part, circle spore mushroom ethyl acetate extract, circle spore mushroom aqueous phase extraction one of position or two kinds are pressed arbitrarily
The mixture of mass ratio.
This circle spore mushroom suppresses the preparation method of hdac1 enzyme effective site it is characterised in that comprising the steps: circle
Spore mushroom is pulverized, and adds concentration expressed in percentage by volume 65% ~ 95% ethanol solution reflux, extract, gained extracting solution is concentrated, is dried, obtain leaching
Cream；By extractum plus distilled water dispersion, obtain extract dispersion liquid, then adopt petroleum ether, ethyl acetate and n-butyl alcohol successively as molten
Agent extract extract dispersion liquid, respectively volatilization remove solvent after must justify spore mushroom petroleum ether part, circle spore mushroom ethyl acetate extract,
Circle spore mushroom n-butanol portion；After extracting, remaining extract dispersion liquid concentrates, is dried, and obtains round spore mushroom aqueous phase extraction position.
Described reflux, extract, is ultrasonic assistant reflux, extract,；The concrete operations of ultrasonic assistant reflux, extract, are: will
Round spore mushroom after pulverizing adds concentration expressed in percentage by volume 65% ~ 95% ethanol solution ultrasonic extraction 0.5 ~ 3 hour, filters, filtering residue
Addition concentration expressed in percentage by volume 65% ~ 95% ethanol solution reflux, extract, 1 ~ 3 time, united extraction liquid, obtain final product.
This circle spore mushroom suppress hdac1 enzyme effective site application it is characterised in that: preparation suppression hdac1 enzyme medicine
The application of aspect.
Described preparation suppression hdac1 enzyme medicine is to pass through: will justify spore mushroom petroleum ether part, circle spore mushroom ethyl acetate portion
Position, circle spore mushroom n-butanol portion, circle spore mushroom aqueous phase extract one of position or two or more by any mass ratio
Mixture, the oral formulations being mixed with pharmaceutic adjuvant or ejection preparation.
Described ejection preparation is lipidosome injection, nanoparticle injection or micro-balloon injection.
Described oral formulations are powder, tablet, granule, capsule or solution
The present invention is described as follows:
In prior art, circle spore mushroom normal usage is treatment cold, fever and skin infection, has no that it has the correlation of antitumor efficacy
Report.Applicant is found by numerous studies: the extracting section thing in circle spore mushroom also has antibacterial, antineoplastic action, but
Be its effect inconspicuous it is impossible to determine its specific effective site.Therefore pass through what kind of solvent, using how preparation method,
The best effective site of suppression hdac1 enzymatic activity effect could be obtained, be the problem having to solve.Applicant passes through research and sends out
Existing: using preparation method of the present invention, the round spore mushroom petroleum ether part filtering out, circle spore mushroom ethyl acetate extract, circle spore mushroom
Mushroom n-butanol portion and circle spore mushroom aqueous phase extraction position, are for suppressing hdac1 enzyme to have preferable effect.
In preparation method, round spore mushroom used is selected from Herb or cap part.Extractum is crude extract, using different molten
Agent extracts extract dispersion liquid, refers to add extract dispersion liquid extract and separate to obtain petroleum ether extraction liquid, incite somebody to action petroleum ether successively
Ethyl acetate adds extract dispersion liquid extract and separate to obtain acetic acid ethyl acetate extract, n-butyl alcohol adds extract dispersion liquid extraction point
From acquisition butanol extraction liquid；Just take round spore mushroom petroleum ether extract, circle spore mushroom acetic acid ethyl acetate extract and circle spore mushroom
Butanol, before immunoassay liquid volatilizees after removal solvent respectively, obtains round spore mushroom petroleum ether part, circle spore mushroom ethyl acetate extract and circle spore
Mushroom n-butanol portion；Remaining extract dispersion liquid (solvent is water) after extracting, volatilization removes solvent and obtains final product round spore mushroom water
Mutually extract position.Aqueous phase extraction position may also be referred to as aqueous phase extract, and aqueous phase extraction position is soluble in water；And relative, stone
Oily ether, ethyl acetate and n-butyl alcohol are organic faciess, and petroleum ether part, ethyl acetate extract are corresponding with n-butanol portion to be soluble in stone
Oily ether, ethyl acetate and n-butyl alcohol.Wherein, circle spore mushroom petroleum ether part contains sterols；Circle spore mushroom ethyl acetate extract
Containing drifting alkaloids, flavone compound；Circle spore mushroom n-butanol portion contains saponin and phenolic compound；Circle spore mushroom water
Mutually polysaccharide and organic acid compound are contained in extraction position.Volatilization remove solvent can using normal heating make solvent (petroleum ether,
Ethyl acetate and n-butyl alcohol) volatilization.Preferably, volatilization removes solvent and adopts concentrating under reduced pressure, the method efficiency high, and is avoided that
Heat-sensitive ingredients lose the property of medicine because of high temperature.
Preferably dosage form is oral formulations or ejection preparation.Oral formulations and ejection preparation are optimal route of administration, herein
Described oral formulations are through intestines and stomach drug-delivery preparation it is preferred that oral formulations are slow-release controlled-release type oral formulations.Can also be
Parenteral routes other, such as respiratory tract administration preparation (spray, aerosol, powder spray)；Percutaneous drug delivery preparation (externally used solution
Agent, lotion, liniment, ointment, plaster, paste, patch)；Film drug-delivery preparation (eye drop, nasal drop, ophthalmic ointment, rinsing the mouth
Agent, sublingual tablet)；Cavity/canal drug administration preparation (suppository).Ejection preparation can be conventional injection preparation.Entered according to drug delivery system
Row classification is it is preferred that the ejection preparation of the present invention is lipidosome injection, nanoparticle injection or micro-balloon injection；Others,
The injection of the present invention can also be microcapsule injection, polymer micelle injection, microemulsion or Submicroemulsion injection, submicron note
Penetrate agent or gel injection, the injection of above drug delivery system can extend pharmaceutical carrier circulation time in vivo, prolongation
Medicine carrying microgranule absorption site the time of staying, control medicine release the initial stage burst effect.Pharmaceutic adjuvant includes medicine and carries
Body, also includes solvent, solubilizing agent, cosolvent, emulsifying agent, suspending agent suspending agent, clarifier, deflocculant, correctivess, coloring
Agent, preservative, chemosterilant, adsorbent, filter aid, antioxidant, ph regulator, isoosmotic adjusting agent, diluent, binding agent,
Wetting agent, disintegrating agent, lubricant, fluidizer, antitack agent, slow releasing agent, controlled release agent, coating material, filmogen, capsule material
One or more of.
Compared with prior art, the round spore mushroom suppression hdac1 enzyme effective site of the present invention and preparation method and application are had
Beneficial effect be:
1st, this circle spore mushroom suppresses hdac1 enzyme effective site can effectively suppress the activity of hdac1 enzyme, for preparation suppression hdac1
Enzyme medicine, has preferable antitumor action.In this area, circle spore mushroom has the work(of relaxing muscles and tendons and activating QI and blood in the collateral, wind-expelling pain-stopping.Applicant
Find that antitumor drug has scarcity of raw material under study for action, and find that circle spore mushroom also has antineoplastic by research
Effect, by designing preparation method, carrying out medicine efficacy screening first on hdac1 enzyme, determines circle spore mushroom rock by numerous studies
One of oily ether position, circle spore mushroom ethyl acetate extract, circle spore mushroom n-butanol portion, circle spore mushroom aqueous phase extraction position
Or the mixture of two or more any mass ratioes is respectively provided with the preferable effect suppressing hdac1 enzyme, hdac1 enzyme survival rate
16.85 ~ 26.38 %, can be used in developing into antitumor Tibetan medicine new drug.Applicant is to have paid substantial amounts of creation in above procedure
Property work.
2nd, this circle spore mushroom suppresses the preparation method of hdac1 enzyme effective site to extract conveniently, and extraction efficiency is high.Preparation method
In, applicant's design adds concentration expressed in percentage by volume 65% ~ 95% ethanol solution to carry out ultrasonic assistant reflux, extract, improves extraction
Efficiency；In extraction, use petroleum ether, ethyl acetate and n-butyl alcohol successively as solvent extraction extract dispersion liquid, using above solvent
The round spore mushroom petroleum ether part of extraction order gained, circle spore mushroom ethyl acetate extract, circle spore mushroom n-butanol portion and circle
Spore mushroom aqueous phase extracts position and is respectively provided with the preferable effect suppressing hdac1 enzyme.
3rd, the present invention has expanded the raw material channel of suppression hdac1 enzyme medicine, expands the purposes of round spore mushroom, makes circle spore mushroom
Mushroom develops into the new raw material of suppression hdac1 enzyme medicine, is remarkably improved the added value of round spore mushroom.
Specific embodiment
Embodiment 1 ~ 3 is the round spore mushroom suppression hdac1 enzyme effective site of the present invention and the specific embodiment of preparation method, its
Middle embodiment 1 is most preferred embodiment.
Embodiment 1
Preparation method, comprises the steps: to pulverize circle spore mushroom, ultrasonic using concentration expressed in percentage by volume 75% ~ 85% ethanol solution
Ripple extracts 1 hour, then filters to obtain ultrasonic extract and filtering residue, adds concentration of volume percent 75% ~ 85% second in filtering residue
Alcoholic solution reflux, extract, 3 times, 0.5 ~ 1 hour every time, filters to obtain reflux extracting liquid, merges ultrasonic extract and reflux, extract,
Liquid, concentrate drying obtain extractum；By extractum plus distilled water dispersion, obtain extract dispersion liquid, then adopt petroleum ether, ethyl acetate successively
With n-butyl alcohol as solvent extraction extract dispersion liquid, volatilization obtains circle spore mushroom petroleum ether part, circle spore mushroom after removing solvent
Ethyl acetate extract, circle spore mushroom n-butanol portion；After extract dispersion liquid is extracted, remaining liq concentrates, is dried, and obtains round spore mushroom
Mushroom aqueous phase extracts position.
Embodiment 2
Preparation method, comprises the steps: to pulverize circle spore mushroom, ultrasonic using concentration expressed in percentage by volume 85% ~ 95% ethanol solution
Ripple extracts 3 hours, then filters to obtain ultrasonic extract and filtering residue, adds concentration of volume percent 85% ~ 95% second in filtering residue
Alcoholic solution reflux, extract, 3 times, 1 ~ 1.5 hour every time, filters to obtain reflux extracting liquid, merges ultrasonic extract and reflux, extract,
Liquid, concentrate drying obtain extractum；By extractum plus distilled water dispersion, obtain extract dispersion liquid, then adopt petroleum ether, ethyl acetate successively
With n-butyl alcohol as solvent extraction extract dispersion liquid, volatilization obtains circle spore mushroom petroleum ether part, circle spore mushroom after removing solvent
Ethyl acetate extract, circle spore mushroom n-butanol portion；After extract dispersion liquid is extracted, remaining liq concentrates, is dried, and obtains round spore mushroom
Mushroom aqueous phase extracts position.
Embodiment 3
Preparation method, comprises the steps: to pulverize circle spore mushroom, ultrasonic using concentration expressed in percentage by volume 65% ~ 75% ethanol solution
Ripple extracts 0.5 hour, then filters to obtain ultrasonic extract and filtering residue, adds concentration of volume percent 65 ~ 75% second in filtering residue
Alcoholic solution reflux, extract, 2 times, 1.5 ~ 2 hours every time, filters to obtain reflux extracting liquid, merges ultrasonic extract and reflux, extract,
Liquid, concentrate drying obtain extractum；By extractum plus distilled water dispersion, obtain extract dispersion liquid, then adopt petroleum ether, ethyl acetate successively
With n-butyl alcohol as solvent extraction extract dispersion liquid, volatilization obtains circle spore mushroom petroleum ether part, circle spore mushroom after removing solvent
Ethyl acetate extract, circle spore mushroom n-butanol portion；After extract dispersion liquid is extracted, remaining liq concentrates, is dried, and obtains round spore mushroom
Mushroom aqueous phase extracts position.
Performance test
Just respectively take the dried round spore mushroom petroleum ether part of 0.4mg, circle spore mushroom ethyl acetate extract, circle spore mushroom respectively
Butanol position, circle spore mushroom aqueous phase extraction position, are each dissolved in formation dmso in the dimethyl sulfoxide (dmso) of 100 l molten
Liquid, ultrasonic 5 minutes.Take the dmso solution of 1 l sample dissolution more respectively, be separately added into buffer 99 l, respectively obtain for examining
Survey the buffer solution of round spore mushroom petroleum ether part of circle spore mushroom suppression hdac1 enzyme, the slow of circle spore mushroom ethyl acetate extract
Rush the buffer solution that solution, the buffer solution of circle spore mushroom n-butanol portion and circle spore mushroom aqueous phase extract position.Buffer
Formula is: the tris-hcl of 50mm/l, the mgcl of kcl, 1mm/l of nacl, 2.7mm/l of 0.137 mm/l2, 0.01% tween
20, buffer ph 8.0.
First, anti-hdac1 enzyme experiment.
Major experimental material is:
Hdac1 enzyme, cisbio bioassays Products；
Tris-hcl, Shanghai Jie Mei gene Pharmaceutical Technology Co., Ltd；
Edta, Shanghai Curie bio tech ltd；
Dtt, Shanghai Sheng Gong biological engineering limited company；
Bsa, Shanghai Jiang Lai bio tech ltd；
Dmso, Shanghai Yi Ka Bioisystech Co., Ltd；
Nacl(analysis is pure), Tianjin red rock chemical reagent factory；
Naoh(analysis is pure), Tianjin Hedong District red rock chemical reagent work.
Major experimental instrument is:
Fluostar omega full-automatic multi-functional microplate reader, Guangdong Province Guangzhou Bai Qi bio tech ltd；
Mp511 type ph meter electricity meter, company limited is believed in Shanghai City three；
Brand8 road liquid-transfering gun, converge your Instrument Ltd. for Hangzhou, Zhejiang province；
500 l liquid-transfering guns, 50 l liquid-transfering guns, Shanghai City Rong Tai biochemical instrument company limited；
Electronic balance xpe105, prunus mume (sieb.) sieb.et zucc. Teller-support benefit Instrument Ltd..
The round spore mushroom being obtained with embodiment 1 suppresses hdac1 enzyme effective site as test medicine.It represents experiment for circle spore
Mushroom suppresses hdac1 enzyme effective site to suppress the activity of hdac1 enzyme.Its method is separately to take the round spore mushroom petroleum ether of 4 l respectively
Buffer solution (label f), buffer solution (label g), the circle spore mushroom n-butyl alcohol portion of circle spore mushroom ethyl acetate extract at position
Position buffer solution (label h) and circle spore mushroom aqueous phase extract position buffer solution (label i), proceeds as follows respectively:
Taken 4 l buffer solution (f, g, h, i) are placed in 96 orifice plates, then are separately added into 2 l hdac1 enzymes, react under room temperature condition
5min, adds 4 l h3(1-21) k9 substrate, 37 DEG C, cultivate 60 minutes in incubator.Add 5 l sa-xl665 and 5 l
H3k9me0 antibody, 665nm measured value, obtain experimental group absorbance.
The buffer solution of 4 l is placed in 96 orifice plates, then is separately added into 2 l hdac1 enzymes, under room temperature condition, react 5min,
Add 4 l h3(1-21) k9 substrate, 37 DEG C, cultivate 60 minutes in incubator.Add 5 l sa-xl665 and 5 l h3k9me0
Antibody, 665nm measured value, obtain matched group absorbance.Calculate hdac1 enzyme survival rate: survival rate (%)=experimental group absorbance/matched group
Absorbance × 100%.
Table 1 circle spore mushroom suppresses hdac1 enzyme effective site to suppress the active testing result of hdac1 enzyme
.
* p ＜ 0.01 in table 1, it can be seen from Table 1 that: circle spore mushroom petroleum ether part, circle spore mushroom ethyl acetate portion
All there is preferable inhibition at position, circle spore mushroom n-butanol portion and circle spore mushroom aqueous phase extraction position for hdac1 enzyme.
2nd, anti-tumor activity test.
Measure the circle spore mushroom suppression cytotoxic activity to human tumor cells for the hdac1 enzyme effective site using mtt method.Treating excess syndrome
Apply the example 1 gained circle each position of spore mushroom appropriate, as test-compound, after dmso dissolving, measured respectively using mtt method and people is tied
Intestinal cancer hct-8, human liver cancer bel-7402 cell, human gastric cancer bgc-803 cell, human lung adenocarcinoma a549 cell and human ovarian cancer
The suppression ratio of a2780 cell growth.
Experimental technique: the cell of exponential phase, after 0.25% pancreatin-edta digestion, is configured to certain density list
Cell suspension, according to the difference of vitro growth rates, is inoculated in 96 orifice plates by 800 ~ 2000/hole, every hole adds cell suspension
100µl.After 24h, add test-compound containing variable concentrations and the fresh culture of coordinative solvent comparison, every hole adds 100 l
(dmso final concentration < 0.1%), every kind of test-compound sets 5 ~ 7 dosage groups, and every group at least sets 3 parallel holes, continues in 37 DEG C
After culture 72h, abandon supernatant, every hole adds the freshly prepared serum-free medium containing 0.5mg/ml mtt of 100 l, continues training
Foster 4h, after abandoning supernatant, every hole adds 200 l dmso dissolving mtt first hairpin precipitations, and microoscillator vibration mixes, and uses microplate reader
Optical density value (od), the tumor cell processing with solvent control is measured under the conditions of reference wavelength 450nm, Detection wavelength 570nm
For matched group, calculate the suppression ratio to tumor cell for the test-compound with below equation, and by middle effect Equation for Calculating ic50: suppression
Rate=(matched group average od value-administration group average od value) ÷ matched group average od value × 100%.By the extractum in preparation method,
And gained round spore mushroom petroleum ether part, circle spore mushroom ethyl acetate extract, circle spore mushroom n-butanol portion, circle spore mushroom
Aqueous phase extraction position carries out tumor cell in vitro cytotoxic activity screening, the results are shown in Table 2.
Table 2 tumor cell in vitro cytotoxic activity the selection result
.
Note: 1), hct-8 be human colon cancer cell, bel-7402 be human liver cancer cell, bgc-823 be gastric carcinoma cells,
A549 is human lung carcinoma cell, and a2780 is Proliferation of Human Ovarian Cell.2), > 50 show do not there is anti-cell cytotoxic activity.Can be seen by table 2
Go out: circle spore mushroom petroleum ether part, circle spore mushroom ethyl acetate extract, circle spore mushroom n-butanol portion, circle spore mushroom aqueous phase extraction
Position is taken really to have preferable antineoplastic effect.
3rd, external s180Tumor-bearing mice anti-tumor experiment.
1st, the s at each position of embodiment 1180Tumor-bearing mice anti-tumor experiment method is as follows:
A) 5 ~ 6 week old kunming mice, selects Mus to be grouped at random, every group 10, every group of male and female half and half (sub-cage rearing)；Group names
For: blank control group, cyclophosphamide group and administration group；Administration group includes: the circle basic, normal, high dosage group of spore mushroom petroleum ether part,
The circle basic, normal, high dosage group of spore mushroom ethyl acetate extract, the circle basic, normal, high dosage group of spore mushroom n-butanol portion, circle spore mushroom
The basic, normal, high dosage group in aqueous phase extraction position；Skin degerming is carried out to every mice；
B) in right fore subcutaneous vaccination s180Tumor liquid 0.2ml(s180Oncocyte number is 2.0 × 106~2.2×106In the range of)；
C) inoculation was administered after 24 hours；Blank control group gastric infusion 10ml/kg injection normal saline；Cyclophosphamide group abdominal cavity
Injection cyclophosphamide 20mg/kg；Administration group adopts the prepared round spore mushroom petroleum ether part of gastric infusion embodiment 1, circle spore mushroom
Mushroom ethyl acetate extract, circle spore mushroom n-butanol portion and circle spore mushroom aqueous phase extraction position；After first administration, the next day claim mice
Body weight first；Every group is administered once daily, and each dosage refers to following table, successive administration 14 days；
D) last dose, after 1 hour, puts to death mice, claims mice last body weight, stripping tumor mass is weighed, and calculates tumour inhibiting rate.Tumour inhibiting rate=
(the average knurl weight of the average knurl weight-administration group of blank control group) average knurl weight × 100% of ÷ blank control group, tumour inhibiting rate is calculated knot
Fruit typing table 3.
The each position of table 3 embodiment 1 is to mice s180The impact of solid tumor
.
2nd, the s at each position of embodiment 2180Tumor-bearing mice method is with embodiment 1, data inputting table 4.
The each position of table 4 embodiment 2 is to mice s180The impact of solid tumor
.
3rd, the s at each position of embodiment 3180The method of tumor-bearing mice anti-tumor experiment is with embodiment 1, data inputting table 5.
The each position of table 5 embodiment 3 is to mice s180The impact of solid tumor
.
Through applicant's statistics:, compared with blank control group, there was no significant difference (p > 0.05) for Mouse Weight change for administration group.
In table 3 ~ 5: s180Refer to mouse ascites oncocyte, s in table 3 ~ 5180Tumor suppression rate score is bigger to represent that antitumous effect is better.By table
3 ~ 5 it can be seen that first, and embodiment 1 ~ 3 all has preferable antitumous effect, in embodiment 1 ~ 3 during same area same dose,
Antitumous effect between each group no significant difference.Secondly, compared with blank control group, circle spore mushroom petroleum ether high dose group, circle
The middle and high dosage group of spore mushroom ethyl acetate extract, circle spore mushroom n-butyl alcohol high dose group, the circle spore mushroom aqueous phase extraction high agent in position
Amount group, Treated with Chemotherapy with Cyclophosphamide group, all can significantly inhibit s180The growth of tumor.
Embodiment 4
Circle spore mushroom suppression hdac1 enzyme effective site is prepared as tablet, using following steps: spore mushroom ethyl acetate portion will be justified
Position 300mg and starch 100mg mixes, plus the gelatinized corn starch 40mg of mass percent concentration 10% makes soft material, and sieve to obtain wet granular,
Dry dry particl, granulate, stiffened fatty acid magnesium 4 mg mixes, tabletting, obtains final product.
Embodiment 5
The preparation method of electuary: 5 parts of position of spore mushroom aqueous phase extraction, 1 part of sucrose, 3 parts of mixings of dextrin will be justified by weight ratio,
Add 2 ~ 5 parts of appropriate mass percent 95% ethanol solution, stirring while adding, prepared soft material, soft material is dried, crosses 16 mesh sieves, point
Dress obtains final product.
Embodiment 6
Pellet capsule is composed of the following raw materials by weight: 30 parts of spore mushroom n-butanol portion of circle, 5 parts of lecithin, Bile Salts 5
Part, 30 parts of Microcrystalline Cellulose；
The preparation method of pellet capsule: spore mushroom n-butanol portion, lecithin, Bile Salts and microcrystalline cellulose will be justified by proportioning
Element is mixed, and pours ethanol water into and stirs evenly acquisition soft material, and soft material is poured into extrusion in extruder, through round as a ball acquisition granule, extrudes
Rotating speed 250r/min, round as a ball rotating speed 800r/min, round as a ball time 20min, are dried, cross 24 ~ 30 mesh sieves acquisition micropills, micropill is filled
Load in capsule shells, obtain final product.
Embodiment 7
The preparation method of lipidosome injection: 1) under nitrogen protection, by 50g cholesterol succinate, 250g distearyl phosphorus
Acyl ethanolamine, 40g soybean lecithin, 50g poloxamer-188 and 10g circle spore mushroom n-butanol portion are dissolved in 1500ml
Volume ratio is in the ethanol of 1:1 and the organic solvent of n-butyl alcohol, and stirring makes it dissolve acquisition suspension；Suspension is passed through decompression
Organic solvent is flung in concentration, obtains immobilized artificial membrane；
2) under nitrogen protection, add the phosphate buffered solution that 8000ml ph is 6.8, stirring in immobilized artificial membrane, make immobilized artificial membrane
Eluting fully swelling hydration, through 0.22 μm of filtering with microporous membrane, obtain the liposome of round spore mushroom n-butanol portion；
3) aseptically, in the liposome of circle spore mushroom n-butanol portion, add 100g trehalose, stir, surpass
Sonicated 0.5 ~ 1 hour, plus water for injection constant volume, through 0.22 μm of filtering with microporous membrane, fill, obtain final product round spore mushroom n-butyl alcohol
The lipidosome injection at position.
Embodiment 8
The preparation method of nanoparticle injection: take 25g circle spore mushroom ethyl acetate extract and 100g Poloxamer 407 3000ml
Anhydrous alcohol solution, adds the ethanol solution of 30ml 1mol/l zinc chloride, stirring mixing, ultrasonic Treatment makes it in 0.5 ~ 1 hour
Dissolving, obtains mixed liquor；Mixed liquor concentrating under reduced pressure is volatilized and removes solvent, put into -20 DEG C of refrigerator freezings 2 hours；Take out filling
Penetrate and use water constant volume, ultrasonic Treatment 0.5 ~ 1 hour, through 0.22 μm of filtering with microporous membrane, fill, obtain final product round spore mushroom ethyl acetate
The nanoparticle injection at position.
The above, be only presently preferred embodiments of the present invention, is not the restriction that the present invention is made with other forms, appoints
What those skilled in the art possibly also with the disclosure above technology contents changed or be modified as equivalent variations etc.
Effect embodiment.But every without departing from technical solution of the present invention content, according to the present invention technical spirit to above example institute
Any simple modification, equivalent variations and the remodeling made, still falls within the protection domain of technical solution of the present invention.
Claims (8)
1. circle spore mushroom suppression hdac1 enzyme effective site it is characterised in that: selected from circle spore mushroom petroleum ether part, circle spore mushroom
Ethyl acetate extract, circle spore mushroom n-butanol portion, circle spore mushroom aqueous phase extract one of position or two or more by appointing
The mixture of meaning mass ratio.
2. the round spore mushroom suppression hdac1 enzyme effective site described in claim 1 it is characterised in that: selected from the circle positive fourth of spore mushroom
Alcohol position and one of circle spore mushroom petroleum ether part, circle spore mushroom ethyl acetate extract, circle spore mushroom aqueous phase extraction position
Or two kinds of mixture by any mass ratio.
3. under the preparation method of the round spore mushroom suppression hdac1 enzyme effective site described in claim 1 is it is characterised in that include
State step: spore mushroom will be justified and pulverize, add concentration expressed in percentage by volume 65% ~ 95% ethanol solution reflux, extract, will be dense for gained extracting solution
Contract, be dried, obtain extractum；By extractum plus distilled water dispersion, obtain extract dispersion liquid, then successively adopt petroleum ether, ethyl acetate and
N-butyl alcohol must justify spore mushroom petroleum ether part, circle spore mushroom as solvent extraction extract dispersion liquid, volatilization respectively after removing solvent
Ethyl acetate extract, circle spore mushroom n-butanol portion；After extracting, remaining extract dispersion liquid concentrates, is dried, and obtains round spore mushroom
Aqueous phase extracts position.
4. the round spore mushroom described in claim 3 suppress hdac1 enzyme effective site preparation method it is characterised in that: described
Reflux, extract, is ultrasonic assistant reflux, extract,；The concrete operations of ultrasonic assistant reflux, extract, are: by the round spore mushroom after pulverizing
Mushroom adds concentration expressed in percentage by volume 65% ~ 95% ethanol solution ultrasonic extraction 0.5 ~ 3 hour, filters, and filtering residue adds volume basis dense
Degree 65% ~ 95% ethanol solution reflux, extract, 1 ~ 3 time, united extraction liquid, obtain final product.
5. the round spore mushroom described in claim 1 suppress hdac1 enzyme effective site application it is characterised in that: in preparation suppression
The application of hdac1 enzyme medicine aspect.
6. round spore mushroom according to claim 5 suppress hdac1 enzyme effective site application it is characterised in that: described system
Standby suppression hdac1 enzyme medicine is to pass through: just will justify spore mushroom petroleum ether part, circle spore mushroom ethyl acetate extract, circle spore mushroom
Butanol position, circle spore mushroom aqueous phase extraction one of position or the two or more mixture by any mass ratio, and medicinal
Oral formulations or ejection preparation that adjuvant is mixed.
7. round spore mushroom according to claim 6 suppress hdac1 enzyme effective site application it is characterised in that: described
Ejection preparation is lipidosome injection, nanoparticle injection or micro-balloon injection.
8. round spore mushroom according to claim 6 suppress hdac1 enzyme effective site application it is characterised in that: described
Oral formulations are powder, tablet, granule, capsule or solution.
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