CN106318927A - Complex enzyme applied into ramie degumming, and preparation method thereof - Google Patents

Complex enzyme applied into ramie degumming, and preparation method thereof Download PDF

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CN106318927A
CN106318927A CN201610702939.1A CN201610702939A CN106318927A CN 106318927 A CN106318927 A CN 106318927A CN 201610702939 A CN201610702939 A CN 201610702939A CN 106318927 A CN106318927 A CN 106318927A
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parts
beta
degumming
bromelain
xylanase
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汪观清
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ANHUI HUALONG HEMP Co Ltd
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ANHUI HUALONG HEMP Co Ltd
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    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
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    • C12N9/2477—Hemicellulases not provided in a preceding group
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    • C12N9/2477—Hemicellulases not provided in a preceding group
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    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
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    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01002—Beta-amylase (3.2.1.2)
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    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
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    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01006—Endo-1,3(4)-beta-glucanase (3.2.1.6)
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    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
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    • C12Y302/01025—Beta-mannosidase (3.2.1.25), i.e. mannanase
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    • C12Y304/22—Cysteine endopeptidases (3.4.22)
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    • C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/02002—Pectate lyase (4.2.2.2)
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01C—CHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
    • D01C1/00—Treatment of vegetable material
    • D01C1/02—Treatment of vegetable material by chemical methods to obtain bast fibres

Abstract

The invention discloses a complex enzyme applied into ramie degumming. The complex enzyme is prepared from the following raw materials in parts by weight: 3-6 parts of beta-glucosidase, 2-5 parts of beta-amylase, 1-4 parts of levansucrase, 12-18 parts of alkaline pectinase, 30-36 parts of beta-glucanase, 10-15 parts of neutral protease, 15-18 parts of xylanase, 15-26 parts of mannase, 18-24 parts of bromelain, 0.1-0.5 part of erythritol, 0.2-0.5 part of 2-hydracrylicacid, 0.2-0.4 part of sodium oxalate, and 25-45 parts of attapulgite clay. The invention further discloses a preparation method of the complex enzyme. The complex enzyme can achieve the effects of stabilizing the degumming effect, reducing the fiber damage, and enhancing the fiber strength after being applied in the ramie degumming, and can be industrially applied in large range.

Description

The compound enzyme being applied in China grass degumming and preparation method
Technical field
The present invention relates to a kind of compound enzyme being applied in China grass degumming and preparation method, belong to textile applications field.
Background technology
Boehmeria (Bochmeria nivea) genus Urticaceae (Urticaceae) perennial root, herbaceos perennial, former Producing China, be referred to as " China's grass " by European and American countries, yield accounts for the 80% of the world.By analyzing Boehmeria composition, its fiber content More than 60%, its phloem fiber is the longest and solid, the longest in known bast-fibre, is a kind of important weaving The raw material of industry.But be attached with substantial amounts of colloid on ramee, account for gross weight 25-35%, mainly pectin, hemicellulose and Lignins etc., according to the difference of ramee variety, its content may be up to 24-45%.Therefore industrial available fiber is obtained, Must carry out degumming process, and the exploitation of fiber degumming direct relation fiber.
Degumming method conventional in the art is chemistry Caustic soda high pressure kiering method, and the method consumes substantial amounts of soda acid,
Along with acid-base raw materials significantly appreciates, cost is caused to go up at double；Meanwhile, chemical Degumming produces substantial amounts of acidic and alkaline waste water, Serious environmental pollution can be caused.To this end, many research worker are primarily focused on a biological and biological chemical combined degumming On.
Japanese Patent Laid-Open No. Sho 51-149976 discloses a kind of method that microorganism carries out China grass degumming, it is indicated that fungus Containing cellulase in the pectase produced, the condition worked is acidity.Because its degumming effect is unstable, and cause fiber strong Degree declines, and industrial being difficult to is applied.The Chinese invention patent application of Application No. CN85104284 discloses and utilizes brood cell's bar Bacterium carries out the method for China grass degumming, and Chinese patent application CN97109044.0 discloses a strain bacillus alcalophilus and is applied to China grass degumming, Chinese patent ZL 01106844.2 discloses a strain Bacillus subtillis CCTCC M200038 and is applied to China grass degumming.Above-mentioned each patent disclosure of that, is all directly used for Boehmeria by the crude enzyme liquid of its described bacterial strain industrialized production Degumming, 2,007,101,146,871 1 kinds of China grass degumming compound enzymic preparations of Chinese patent disclose a kind of enzyme preparation and take off for Boehmeria Glue, but do not take into account glue qualitative diversity appended by ramee and the situation of pectinase activity height, commercial Application is deposited In problems.
Summary of the invention
For overcoming above-mentioned deficiency, it is an object of the present invention to provide a kind of compound enzyme being applied in China grass degumming, use this multiple Synthase is applied in China grass degumming, can play degumming effect and stablize, reduces fibre damage, the effect of raising fibre strength, and Industrial applications on a large scale can be carried out.
The solution of the present invention is as follows: the compound enzyme being applied in China grass degumming, is composed of the following raw materials by weight:
Beta-glucosidase 3-6 part, beta amylase 2-5 part, levansucrase 1-4 part, alkaline pectase 12-18 part, β-Portugal Dextranase 30-36 part, neutral protease 10-15 part, xylanase 15-18 part, mannase 15-26 part, bromelain 18- 24 parts, erythritol 0.1-0.5 part, 2 hydroxy propanoic acid 0.2-0.5 part, Disodium oxalate. 0.2-0.4 part and attapulgite clay 25-45 Part.
Further, it is composed of the following raw materials by weight: beta-glucosidase 4 parts, beta amylase 3 parts, levansucrase 2 Part, alkaline pectase 16 parts, 1,4 beta-glucanase 33 parts, neutral protease 12 parts, xylanase 16 parts, mannase 19 parts, Bromelain 20 parts, erythritol 0.2 part, 2 hydroxy propanoic acid 0.3 part, Disodium oxalate. 0.3 part and attapulgite clay 32 parts.
Further, it is composed of the following raw materials by weight: beta-glucosidase 3 parts, beta amylase 5 parts, Collagenase 2 Part, levansucrase 2 parts, alkaline pectase 16 parts, 1,4 beta-glucanase 32 parts, neutral protease 12 parts, xylanase 16 Part, mannase 22 parts, bromelain 19 parts, erythritol 0.25 part, 2 hydroxy propanoic acid 0.22 part, Disodium oxalate. 0.23 part and recessed Convex rod clay 40 parts.
Further, it is composed of the following raw materials by weight: beta-glucosidase 5 parts, beta amylase 2 parts, levansucrase 3 Part, alkaline pectase 15 parts, 1,4 beta-glucanase 34 parts, neutral protease 12 parts, xylanase 16 parts, mannase 22 parts, Bromelain 22 parts, erythritol 0.3 part, 2 hydroxy propanoic acid 0.4 part, Disodium oxalate. 0.35 part and attapulgite clay 28 parts.
The compound enzyme preparation method being applied in China grass degumming, by each raw material mix homogeneously at normal temperatures, is heated to 35-40 DEG C stir, mixing time 1-1.5h, i.e. arrives compound enzyme.
Enzyme activity selected by the present invention is as follows: beta-glucosidase 5000U/ml, beta amylase 6000U/ml, Collagenase 80000U/ml, levansucrase 10000U/ml, alkaline pectase 300U/ml, 1,4 beta-glucanase 4000U/ml, neutral protease 600U/ml, xylanase 500U/ml, mannase 600U/ml, bromelain 50000U/ ml。
Present invention bromelain enhances alkaline pectase, glucanase, neutral protease, xylanase, mannan The hydrolysis to pectin of enzyme so that it is be converted into little differentiation compound；Beta amylase can improve the hydrolysis of bromelain, causes neutral fruit Xanthan molecule solvation degree is greatly improved, and improves its hydrolysis to hemicellulose.
Present invention attapulgite clay provides carrier for enzyme preparation, adsorb when enzyme preparation plays a role simultaneously pectin and Catabolite, erythritol is conducive to improving the service efficiency of Collagenase, and 2 hydroxy propanoic acid, Disodium oxalate. are conducive to improving recessed The adsorption efficiency of convex rod clay.Comparing tradition enzyme preparation, degumming efficiency is greatly improved.
The method of the invention has cut the step pickling in traditional method, instead of the step soda boiling in traditional handicraft, Saving substantial amounts of acid-base raw materials, have treatment conditions gentle, low cost, fiber quality is good, the advantages such as environmental pollution is little, phase The traditional enzyme preparation of ratio, in hgher efficiency.
Detailed description of the invention
Below in conjunction with embodiment, the present invention will be further described.
Beta-glucosidase used in the present invention, beta amylase, 1,4 beta-glucanase, neutral protease, xylanase by Shi Feng bio tech ltd, Shanghai produces, and alkaline pectase is that Shanghai KDN Biotech Co. Ltd. produces, and fruit is poly- Sucrase enzyme, mannase be any main component and any producer of vigor the most commercially available；Bromelain is by Dong Henghua road, Nanning Biotechnology Co., Ltd produces.
Embodiment 1
By beta-glucosidase 4 grams, beta amylase 3 grams, levansucrase 2 grams, alkaline pectase 16 grams, 1,4 beta-glucanase 33 Gram, neutral protease 12 grams, xylanase 16 grams, mannase 19 grams, bromelain 20 grams, erythritol 0.2 gram, 2-hydroxyl Base propanoic acid 0.3 gram, Disodium oxalate. 0.3 gram and attapulgite clay 32 grams, mix homogeneously at normal temperatures, be heated to 35 DEG C and stir, Mixing time 1h, obtains compound enzyme.
Apply this example according to by Boehmeria binding, dress cage, enzyme refining, retted fibre, wash fiber crops, soda boiling, copy fiber crops, rinsing, oil supply, removing oil Water, trembling fiber crops, drying, obtain degummed ramie, described enzyme refining step, its main control parameters is: compound enzymic preparation addition 1- 3%, body lotion is than for 1:15, pH8.5-9.5, and degumming temperature controls at 40 DEG C, and degummase liquid is kept stirring for state, usually time For 1h.Measuring ramee uses compound enzyme according to data after PROCESS FOR TREATMENT of the present invention.
Table 1 present example 1 tests data for ramee
The continuous three crowdes of average/% of qualification rate Residual gum content/% Fibre strength (gf/D) Runner rate/% Oil-containing/% Fibre count
Index of the present invention ≥ 99 1.52 5.03 7.18 0.771 2235
Certified products index ≥ 90 ＜ 2.8% ＞ 4.5 ＜ 10% 0.6~ 1.8 Nothing
Embodiment 2
By beta-glucosidase 3 grams, beta amylase 5 grams, Collagenase 2 grams, levansucrase 2 grams, alkaline pectase 16 Gram, 1,4 beta-glucanase 32 grams, neutral protease 12 grams, xylanase 16 grams, mannase 22 grams, bromelain 19 grams, red moss Sugar alcohol 0.25 gram, 2 hydroxy propanoic acid 0.22 gram, Disodium oxalate. 0.23 gram and attapulgite clay 40 grams mix homogeneously at normal temperatures, heating Stir to 38 DEG C, mixing time 1.2h, i.e. arrives compound enzyme.
Apply this example according to by Boehmeria binding, dress cage, enzyme refining, retted fibre, wash fiber crops, soda boiling, copy fiber crops, rinsing, oil supply, removing oil Water, trembling fiber crops, drying, obtain degummed ramie, described enzyme refining step, its main control parameters is: compound enzymic preparation addition 1- 3%, body lotion is than for 1:15, pH8.5-9.5, and degumming temperature controls at 40 DEG C, and degummase liquid is kept stirring for state, usually time For 1h.Measuring ramee uses compound enzyme according to data after PROCESS FOR TREATMENT of the present invention.
Table 2 present example 2 tests data for ramee
The continuous three crowdes of average/% of qualification rate Residual gum content/% Fibre strength (gf/D) Runner rate/% Oil-containing/% Fibre count
Index of the present invention ≥ 99 0.547 5.66 4.248 0.69 2079
Certified products index ≥ 90 ＜ 2.8% ＞ 4.5 ＜ 10% 0.6~ 1.8 Nothing
Embodiment 3
By beta-glucosidase 5 grams, beta amylase 2 grams, levansucrase 3 grams, alkaline pectase 15 grams, 1,4 beta-glucanase 34 Gram, neutral protease 12 grams, xylanase 16 grams, mannase 22 grams, bromelain 22 grams, erythritol 0.3 gram, 2-hydroxyl Base propanoic acid 0.4 gram, Disodium oxalate. 0.35 gram and attapulgite clay 28 grams mix homogeneously at normal temperatures, be heated to 36 DEG C and stir, Mixing time 1.5h, i.e. arrives compound enzyme.
Apply this example according to by Boehmeria binding, dress cage, enzyme refining, retted fibre, wash fiber crops, soda boiling, copy fiber crops, rinsing, oil supply, removing oil Water, trembling fiber crops, drying, obtain degummed ramie, described enzyme refining step, its main control parameters is: compound enzymic preparation addition 1- 3%, body lotion ratio is for 1:15, pH8.5-9.5, and adding Disodium oxalate. is 1% and bentonite 2%, and degumming temperature controls at 40 DEG C, degumming Enzyme liquid is kept stirring for state, and usually time is 1h.After measurement ramee uses compound enzyme according to PROCESS FOR TREATMENT of the present invention Data.
Table 3 present example 3 tests data for ramee
The continuous three crowdes of average/% of qualification rate Residual gum content/% Fibre strength (gf/D) Runner rate/% Oil-containing/% Fibre count
Index of the present invention ≥ 98 0.18 6.13 3.35 0.68 2081
Certified products index ≥ 90 ＜ 2.8% ＞ 4.5 ＜ 10% 0.6~ 1.8 Nothing
After above-described embodiment, the ramee test data of gained are seen can be seen that, apply compound enzyme of the present invention to process Boehmeria Refining glue, the ramee property indices of gained is the most excellent.
Although the detailed description of the invention to the present invention gives detailed description and illustrates above, but it should be noted that We can carry out various equivalence according to the conception of the present invention to above-mentioned embodiment and change and amendment, and function produced by it is made With still without departing from description contained spiritual time, all should be within protection scope of the present invention.

Claims (5)

1. it is applied to the compound enzyme in China grass degumming, it is characterised in that be composed of the following raw materials by weight:
Beta-glucosidase 3-6 part, beta amylase 2-5 part, levansucrase 1-4 part, alkaline pectase 12-18 part, β-Portugal Dextranase 30-36 part, neutral protease 10-15 part, xylanase 15-18 part, mannase 15-26 part, bromelain 18- 24 parts, erythritol 0.1-0.5 part, 2 hydroxy propanoic acid 0.2-0.5 part, Disodium oxalate. 0.2-0.4 part and attapulgite clay 25-45 Part.
The compound enzyme being applied in China grass degumming the most according to claim 1, it is characterised in that by following raw material Composition: beta-glucosidase 4 parts, beta amylase 3 parts, levansucrase 2 parts, alkaline pectase 16 parts, 1,4 beta-glucanase 33 Part, neutral protease 12 parts, xylanase 16 parts, mannase 19 parts, bromelain 20 parts, erythritol 0.2 part, 2-hydroxyl Base propanoic acid 0.3 part, Disodium oxalate. 0.3 part and attapulgite clay 32 parts.
The compound enzyme being applied in China grass degumming the most according to claim 1, it is characterised in that by following raw material Composition: beta-glucosidase 3 parts, beta amylase 5 parts, levansucrase 2 parts, alkaline pectase 16 parts, 1,4 beta-glucanase 32 Part, neutral protease 12 parts, xylanase 16 parts, mannase 22 parts, bromelain 19 parts, erythritol 0.25 part, 2-hydroxyl Base propanoic acid 0.22 part, Disodium oxalate. 0.23 part and attapulgite clay 40 parts.
The compound enzyme being applied in China grass degumming the most according to claim 1, it is characterised in that by following raw material Composition: beta-glucosidase 5 parts, beta amylase 2 parts, levansucrase 3 parts, alkaline pectase 15 parts, 1,4 beta-glucanase 34 Part, neutral protease 12 parts, xylanase 16 parts, mannase 22 parts, bromelain 22 parts, erythritol 0.3 part, 2-hydroxyl Base propanoic acid 0.4 part, Disodium oxalate. 0.35 part and attapulgite clay 28 parts.
5. according to the preparation method of the arbitrary described compound enzyme being applied in China grass degumming of claim 1-4, it is characterised in that By each raw material mix homogeneously at normal temperatures, it is heated to 35-40 DEG C and stirs, mixing time 1-1.5h, i.e. arrives compound enzyme.
CN201610702939.1A 2016-08-22 2016-08-22 Complex enzyme applied into ramie degumming, and preparation method thereof Pending CN106318927A (en)

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Application publication date: 20170111