CN106305705A - Preparation method for diluent for milk goat artificial insemination technology - Google Patents
Preparation method for diluent for milk goat artificial insemination technology Download PDFInfo
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- CN106305705A CN106305705A CN201610607154.6A CN201610607154A CN106305705A CN 106305705 A CN106305705 A CN 106305705A CN 201610607154 A CN201610607154 A CN 201610607154A CN 106305705 A CN106305705 A CN 106305705A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0215—Disinfecting agents, e.g. antimicrobials for preserving living parts
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
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Abstract
The invention provides a preparation method for a diluent for the milk goat artificial insemination technology. The method comprises the steps that glucose, sodium citrate and disodium edetate dehydrate are poured into a container, distilled water is added into the container, and enrofloxacin is added into the mixed solution to obtain a basic solution; vitamin C, vitamin B12, oxytocin and prostaglandin are added into an ethylene glycol solution to obtain a protective solution; then, kanamycin sulfate is added into skimmed goat milk to obtain a nutrient solution; finally, the basic solution, the protective solution and the nutrient solution are mixed and stirred to be uniform under the sterile condition at low temperature of 4 DEG C to obtain the diluent for the milk goat artificial insemination technology. The preparation method has the advantages that the semen dilution ratio can reach up to 30 times, sperm activity can still be kept at 0.3-0.4 120 h after semen is diluted, and it can be ensured that the ewe artificial insemination hybridization pregnancy rate is 85% or above.
Description
Technical field
The present invention relates to biological technical field, be specifically related to the preparation method of milk goat technology of artificial insemination diluent.
Background technology
Semen cryopreservation plays very important effect with artificial fertilization technology in modernization Animal husbandry production, should
Carry out artificial insemination with frozen semen to be limited by time, region, kind and individuality, and reduce raising of male animal in a large number
Support quantity, reduce production cost, improve the utilization rate of good species male animal, improve the economic benefit of aquaculture.China milch cow and
What on beef cattle, application frozen semen technology and technology of artificial insemination had developed is the most perfect, promotes at milk goat technology of artificial insemination
During, due to the diluent poor effect of milk goat artificial insemination on market so that technology of artificial insemination Difficulty is heavy.
In the semen dilution link of artificial insemination, existing milk goat diluent there is problems in that semen dilution rate is low, the most only
About 3 times can be diluted；After semen dilution, Sperm survival time is short, and typically within 12-24 hour, semen dilution is essence after 24 hours
Sub-vigor reduces, below 0.3；Artificial insemination ewe conception rate is low, typically about 65%.
Summary of the invention
It is an object of the invention to provide the preparation method of a kind of milk goat technology of artificial insemination diluent, existing to overcome
Milk goat diluent semen dilution rate is low, Sperm survival time is short after semen dilution, motility of sperm is low and artificial after dilution
The problem that insemination ewe conception rate is low.
The technical solution adopted in the present invention is:
The preparation method of milk goat technology of artificial insemination diluent, its concrete operation step is as follows:
Step 1, configures basal liquid
Under conditions of ambient temperature is 35～40 DEG C, weigh glucose 3～5g, sodium citrate 1～2g and ethylenediamine tetraacetic
Acetic acid disodium 0.05～0.2g, pours into these three material in a container, adds distilled water to 100ml in container, and stirring is all
Even, obtain mixed solution；In this mixed solution, add enrofloxacin again, stir, obtain basal liquid；Every 100mL mixing
Solution adds 5000UG enrofloxacin；Finally by basal liquid at 4 DEG C of cryopreservation；
Step 2, configuration protection liquid
Under conditions of ambient temperature is 35～40 DEG C, configuration quality mark is the ethylene glycol solution of 10%～15%；Again
Vitamin C, vitamin B is added in ethylene glycol solution12, oxytocin and prostaglandin；Every 1ml ethylene glycol solution add 0.03～
0.10ml vitamin C, 0.01～0.5ml vitamin B12, 0.01～0.5ml oxytocin and 0.01～0.5ml prostaglandin, obtain
Protection liquid；Liquid will be protected at 4 DEG C of cryopreservation；
Step 3, configures nutritional solution
Under conditions of ambient temperature is 35～40 DEG C, in the defat sheep milk that mass fraction is 5% add sulphuric acid card that
Mycin, obtains nutritional solution；Wherein, 100ml volume defat sheep milk adds 100,000 IU kanamycin sulfate；Nutritional solution is low at 4 DEG C
Temperature preserves；
Step 4, configures semen diluent
By basal liquid, protection liquid and nutritional solution mixing and stirring under the aseptic condition of 4 DEG C of low temperature, obtain milk goat people
Work insemination techniques diluent.
Above-mentioned mass fraction is the defat sheep milk of 5%, and its solute is the fresh sheep milk after defat, and solvent is distilled water.
In step 4, by mass percentage, weigh the basal liquid of 60～80%, 5～the protection liquid of 15% and 15～25%
Nutritional solution, the mass percent summation of above each component is 100%；These three material is mixed under the aseptic condition of 4 DEG C of low temperature
Conjunction stirs, and obtains milk goat technology of artificial insemination diluent.
The invention has the beneficial effects as follows, semen dilution rate may be up to 30 times, and after semen dilution, the holding time is little up to 120
Time, semen dilution after 24 hours motility of sperm reach 0.5-0.7, semen dilution after 120 hours motility of sperm still remain in
0.3-0.4, it is ensured that ewe artificial insemination conception rate is more than 85%；And diluent collocation method is simple, it is simple to technology people
Member grasps, and can effectively promote fanning economics and the popularization of artificial insemination fresh essence technology.
Detailed description of the invention
The present invention provides the preparation method of a kind of milk goat technology of artificial insemination diluent, and its concrete operation step is such as
Under:
Step 1, configures basal liquid
Under conditions of ambient temperature is 35～40 DEG C, weigh glucose 3～5g, sodium citrate 1～2g and ethylenediamine tetraacetic
Acetic acid disodium 0.05～0.2g, pours into these three material in a container, adds distilled water to 100ml in container, and stirring is all
Even, obtain mixed solution；In this mixed solution, add enrofloxacin again, stir, obtain basal liquid；Every 100mL mixes
Close and solution adds 5000UG enrofloxacin；Finally by basal liquid at 4 DEG C of cryopreservation；
Step 2, configuration protection liquid
Under conditions of ambient temperature is 35～40 DEG C, configuration quality mark is the ethylene glycol solution of 10%～15%；Again
Vitamin C, vitamin B is added in ethylene glycol solution12, oxytocin and prostaglandin；Every 1ml ethylene glycol solution add 0.03～
0.10ml vitamin C, 0.01～0.5ml vitamin B12, 0.01～0.5ml oxytocin and 0.01～0.5ml prostaglandin, obtain
Protection liquid；Liquid will be protected at 4 DEG C of cryopreservation；
Step 3, configures nutritional solution
Under conditions of ambient temperature is 35～40 DEG C, in the defat sheep milk that mass fraction is 5% add sulphuric acid card that
Mycin, obtains nutritional solution；Wherein, 100ml volume defat sheep milk adds 100,000 IU kanamycin sulfate；Nutritional solution is low at 4 DEG C
Temperature preserves；It addition, the defat sheep milk that mass fraction is 5%, its solute is the fresh sheep milk after defat, and solvent is distilled water.
Step 4, configures semen diluent
By mass percentage, weigh the basal liquid of 60～80%, 5～the protection liquid of 15% and 15～the nutritional solution of 25%,
The mass percent summation of above each component is 100%；By equal for these three material mix and blend under the aseptic condition of 4 DEG C of low temperature
Even, obtain milk goat technology of artificial insemination diluent.
In basal liquid, glucose acts not only as the nutrient substance of sperm, and it can also effectively suppress lecithinase simultaneously
Activity, prevent the accumulation of LYSOLECITHIN SUNLECITHIN A in seminal fluid.Owing to glucose is small-molecule substance, can significantly modify oozing of diluent
Pressure, can play a protective role to sperm thoroughly；Sodium citrate and disodiumedetate, Main Function is that buffered sperm is in dilution
The harmful effect that the refuse produced due to metabolism during preservation makes the pH of seminal fluid change and brought motility of sperm, it is also possible to
Relax the diluent intermediate ion detrimental effect to sperm, extend Sperm survival time；Enrofloxacin is slightly yellow or faint yellow knot
Crystalline substance powder, long half time, there is good tissue distribution in animal body, belong to broad-spectrum antibacterial, for gram sun
Property bacterium, negative bacterium have bacteriostasis.
In protection liquid, ethylene glycol is more satisfactory sperm cryoprotective agent, can penetrate into inside spermatid, regulation
The balance of the inside and outside osmotic pressure of spermatid, shields to sperm；Vitamin C is a kind of antioxidant, and protection sperm avoids
The threat of free radical, also serves as a kind of coenzyme simultaneously, participates in the various metabolism of sperm；Vitamin B12It it is a kind of physiologically active thing
Matter, as the coenzyme of mutases several in sperm, extends Sperm survival time, improves sperm ovum binding probability；Oxytocin and prostatitis
Parathyrine can improve female animal conception rate: promotes female animal uterine myometrium, improves Sperm vectors, prevents seminal fluid from flowing backwards, have
Effect promotes sperm ovum binding probability.
In nutritional solution, containing rich in protein in defat sheep milk, have maintaining seminal fluid pH and some metal ions of sequestration
Well effect, and when can effectively delay Semen routine, low temperature hits, and significantly improves the motility of sperm after dilution；Sulphuric acid card that
Mycin is aminoglycosides broad ectrum antibiotic, mainly the S. aureus L-forms that gram-negative bacteria and drug resistance are strong is also had good antibacterial work
With.
Below in conjunction with detailed description of the invention, the present invention will be described in detail.
Embodiment 1
A kind of preparation method of milk goat technology of artificial insemination diluent, its concrete operation step is as follows:
Step 1, configures basal liquid
Under conditions of ambient temperature is 40 DEG C, weigh glucose 3g, sodium citrate 2g and disodiumedetate
0.1g, pours into these three material in a container, adds distilled water to 100ml, stir in container, obtains mixing molten
Liquid；In this mixed solution, add enrofloxacin again, stir, obtain basal liquid；Every 100mL mixed solution adds
5000UG enrofloxacin；Finally by basal liquid at 4 DEG C of cryopreservation；
Step 2, configuration protection liquid
Under conditions of ambient temperature is 40 DEG C, configuration quality mark is the ethylene glycol solution of 11%；Molten toward ethylene glycol again
Liquid adds vitamin C, vitamin B12, oxytocin and prostaglandin；Every 1ml ethylene glycol solution adds 0.1ml vitamin C,
0.15ml vitamin B12, 0.01ml oxytocin and 0.08ml prostaglandin, obtain protecting liquid；Liquid will be protected at 4 DEG C of cryopreservation；
Step 3, configures nutritional solution
Under conditions of ambient temperature is 40 DEG C, the defat sheep milk that mass fraction is 5% adds kanamycin sulfate,
Obtain nutritional solution；Wherein, 100ml volume defat sheep milk adds 100,000 IU kanamycin sulfate；Nutritional solution is protected at 4 DEG C of low temperature
Deposit；
Step 4, configures semen diluent
By mass percentage, weighing the basal liquid of 70%, the protection liquid of 10% and the nutritional solution of 20%, by these three material
Mixing and stirring under the aseptic condition of 4 DEG C of low temperature, obtains milk goat technology of artificial insemination diluent.
Embodiment 2
A kind of preparation method of milk goat technology of artificial insemination diluent, its concrete operation step is as follows:
Step 1, configures basal liquid
Under conditions of ambient temperature is 35 DEG C, weigh glucose 3.5g, sodium citrate 1.5g and ethylenediaminetetraacetic acid two
Sodium 0.05g, pours into these three material in a container, adds distilled water to 100ml, stir, mixed in container
Solution；In this mixed solution, add enrofloxacin again, stir, obtain basal liquid；Every 100mL mixed solution adds
5000UG enrofloxacin；Finally by basal liquid at 4 DEG C of cryopreservation；
Step 2, configuration protection liquid
Under conditions of ambient temperature is 35 DEG C, configuration quality mark is the ethylene glycol solution of 13%；Molten toward ethylene glycol again
Liquid adds vitamin C, vitamin B12, oxytocin and prostaglandin；Every 1ml ethylene glycol solution adds 0.08ml vitamin C,
0.5ml vitamin B12, 0.1ml oxytocin and 0.01ml prostaglandin, obtain protecting liquid；Liquid will be protected at 4 DEG C of cryopreservation；
Step 3, configures nutritional solution
Under conditions of ambient temperature is 35 DEG C, the defat sheep milk that mass fraction is 5% adds kanamycin sulfate,
Obtain nutritional solution；Wherein, 100ml volume defat sheep milk adds 100,000 IU kanamycin sulfate；Nutritional solution is protected at 4 DEG C of low temperature
Deposit；It addition, the defat sheep milk that mass fraction is 5%, its solute is the fresh sheep milk after defat, and solvent is distilled water.
Step 4, configures semen diluent
By mass percentage, weighing the basal liquid of 60%, the protection liquid of 15% and the nutritional solution of 25%, by these three material
Mixing and stirring under the aseptic condition of 4 DEG C of low temperature, obtains milk goat technology of artificial insemination diluent.
Embodiment 3
A kind of preparation method of milk goat technology of artificial insemination diluent, its concrete operation step is as follows:
Step 1, configures basal liquid
Under conditions of ambient temperature is 37 DEG C, weigh glucose 4.8g, sodium citrate 1.2g and ethylenediaminetetraacetic acid two
Sodium 0.18g, pours into these three material in a container, adds distilled water to 100ml, stir, mixed in container
Solution；In this mixed solution, add enrofloxacin again, stir, obtain basal liquid；Every 100mL mixed solution adds
5000UG enrofloxacin；Finally by basal liquid at 4 DEG C of cryopreservation；
Step 2, configuration protection liquid
Under conditions of ambient temperature is 37 DEG C, configuration quality mark is the ethylene glycol solution of 15%；Molten toward ethylene glycol again
Liquid adds vitamin C, vitamin B12, oxytocin and prostaglandin；Every 1ml ethylene glycol solution adds 0.04ml vitamin C,
0.01ml vitamin B12, 0.36ml oxytocin and 0.5ml prostaglandin, obtain protecting liquid；Liquid will be protected at 4 DEG C of cryopreservation；
Step 3, configures nutritional solution
Under conditions of ambient temperature is 37 DEG C, the defat sheep milk that mass fraction is 5% adds kanamycin sulfate,
Obtain nutritional solution；Wherein, 100ml volume defat sheep milk adds 100,000 IU kanamycin sulfate；Nutritional solution is protected at 4 DEG C of low temperature
Deposit；It addition, the defat sheep milk that mass fraction is 5%, its solute is the fresh sheep milk after defat, and solvent is distilled water；
Step 4, configures semen diluent
By mass percentage, weighing the basal liquid of 80%, the protection liquid of 5% and the nutritional solution of 15%, by these three material
Mixing and stirring under the aseptic condition of 4 DEG C of low temperature, obtains milk goat technology of artificial insemination diluent.
Embodiment 4
A kind of preparation method of milk goat technology of artificial insemination diluent, its concrete operation step is as follows:
Step 1, configures basal liquid
Under conditions of ambient temperature is 38 DEG C, weigh glucose 5g, sodium citrate 1g and disodiumedetate
0.2g, pours into these three material in a container, adds distilled water to 100ml, stir in container, obtains mixing molten
Liquid；In this mixed solution, add enrofloxacin again, stir, obtain basal liquid；Every 100mL mixed solution adds
5000UG enrofloxacin；Finally by basal liquid at 4 DEG C of cryopreservation；
Step 2, configuration protection liquid
Under conditions of ambient temperature is 38 DEG C, configuration quality mark is the ethylene glycol solution of 10%；Molten toward ethylene glycol again
Liquid adds vitamin C, vitamin B12, oxytocin and prostaglandin；Every 1ml ethylene glycol solution adds 0.03ml vitamin C,
0.3ml vitamin B12, 0.5ml oxytocin and 0.27ml prostaglandin, obtain protecting liquid；Liquid will be protected at 4 DEG C of cryopreservation；
Step 3, configures nutritional solution
Under conditions of ambient temperature is 38 DEG C, the defat sheep milk that mass fraction is 5% adds kanamycin sulfate,
Obtain nutritional solution；Wherein, 100ml volume defat sheep milk adds 100,000 IU kanamycin sulfate；Nutritional solution is protected at 4 DEG C of low temperature
Deposit；It addition, the defat sheep milk that mass fraction is 5%, its solute is the fresh sheep milk after defat, and solvent is distilled water；
Step 4, configures semen diluent
By mass percentage, weighing the basal liquid of 65%, the protection liquid of 13% and the nutritional solution of 22%, by these three material
Mixing and stirring under the aseptic condition of 4 DEG C of low temperature, obtains milk goat technology of artificial insemination diluent.
Using method: 1. gather stud ram seminal fluid；2. examine under a microscope whether this seminal fluid meets the requirements, filter out conjunction
The ram seminal fluid of lattice, Eligibility requirements is: fresh semen vigor is more than 0.8 and density is more than 2.0 × 109The seminal fluid of individual/mL；3. exist
Under the low temperature environment of 4 DEG C, diluent of the present invention and seminal fluid being mixed in the ratio of 30:1, the effective sperm count after dilution is 0.6
×108Individual/more than mL.
After diluent of the present invention and seminal fluid being mixed, carry out 4 DEG C of cryogenic sterile environment storages, depositing through different time
Storage, detects that the performance of sperm is as shown in table 1 after dilution:
Table 1 semen dilution performance test
Sperm survival time: Sperm survival time refers to total time-to-live of sperm sperm under certain condition, with fertilization
Ability is relevant, and the time-to-live is the longest, and sperm fertilizing ability is the strongest.The review time of Sperm survival time=for the first time is to the most second from the bottom
Time interval between secondary inspection+last half with second from the bottom check time interval.
Sperm survival index: sperm survival index is the comprehensive anti-of Sperm survival time and two kinds of indexs of living spermatozoa percentage
Reflect.The summation of the product addition of the sperm average vigor time with interval of sperm survival index=adjacent twice inspection front and back.
From table 1 it will be seen that with diluent of the present invention to qualified milk goat semen dilution after, 4 DEG C of low temperature without
Under the storage condition of bacterium, can preserve 120 hours；In 120 hours, the seminal fluid of dilution complies fully with insemination requirement.
It addition, this diluent belongs to fresh essence diluent, the seminal fluid of fresh essence technical configuration is liquid.
In the artificial insemination procedures of milk goat field, planting 30 times of volume diluted of the present invention of sheep seminal fluid, ewe oestruses
After, the most each semen deposition once: ewe full group conception rate is more than 85%, with the conception rate of ewe natural mating without significant difference,
Semen dilution 120 hours, motility of sperm stills remain in 0.3-0.4, and the breeding in month of 1000 ewes all terminates, and only 7
Stud ram recycles, and just can meet needs of breeding, and not affect stud ram production performance.
Claims (3)
1. the preparation method of milk goat technology of artificial insemination diluent, it is characterised in that its concrete operation step is as follows:
Step 1, configures basal liquid
Under conditions of ambient temperature is 35～40 DEG C, weigh glucose 3～5g, sodium citrate 1～2g and ethylenediaminetetraacetic acid
Disodium 0.05～0.2g, pours into these three material in a container, adds distilled water to 100ml, stir in container,
To mixed solution；In this mixed solution, add enrofloxacin again, stir, obtain basal liquid；In every 100mL mixed solution
Add 5000UG enrofloxacin；Finally by basal liquid at 4 DEG C of cryopreservation；
Step 2, configuration protection liquid
Under conditions of ambient temperature is 35～40 DEG C, configuration quality mark is the ethylene glycol solution of 10%～15%；Again toward second
Glycol solution adds vitamin C, vitamin B12, oxytocin and prostaglandin；Every 1ml ethylene glycol solution add 0.03～
0.10ml vitamin C, 0.01～0.5ml vitamin B12, 0.01～0.5ml oxytocin and 0.01～0.5ml prostaglandin, obtain
Protection liquid；Liquid will be protected at 4 DEG C of cryopreservation；
Step 3, configures nutritional solution
Under conditions of ambient temperature is 35～40 DEG C, the defat sheep milk that mass fraction is 5% adds kanamycin sulfate,
Obtain nutritional solution；Wherein, 100ml volume defat sheep milk adds 100,000 IU kanamycin sulfate；Nutritional solution is protected at 4 DEG C of low temperature
Deposit；
Step 4, configures semen diluent
By basal liquid, protection liquid and nutritional solution mixing and stirring under the aseptic condition of 4 DEG C of low temperature, obtain milk goat and manually award
Essence technology diluent.
The preparation method of diluent the most according to claim 1, it is characterised in that: described mass fraction is the defat sheep of 5%
Milk, its solute is the fresh sheep milk after defat, and solvent is distilled water.
The preparation method of diluent the most according to claim 1, it is characterised in that: in described step 4, by mass percentage,
Weigh the basal liquid of 60～80%, 5～the protection liquid of 15% and 15～the nutritional solution of 25%, the mass percent of above each component
Summation is 100%；By these three material mixing and stirring under the aseptic condition of 4 DEG C of low temperature, obtain milk goat artificial insemination
Technology diluent.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108094409A (en) * | 2018-01-12 | 2018-06-01 | 刘铮铸 | The preparation method and applications of ram freezing semen dilution |
| CN108452290A (en) * | 2018-06-21 | 2018-08-28 | 陕西奥能生物科技有限公司 | A kind of embryo gender interference preparation of sheep, preparation method and applications |
| CN109221091A (en) * | 2018-11-01 | 2019-01-18 | 洛宁农本畜牧科技开发有限公司 | Fresh smart low temperature dilution of a kind of animal and the preparation method and application thereof |
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| CN103518706A (en) * | 2013-10-21 | 2014-01-22 | 西北农林科技大学 | Diluent of frozen semen of milk goat, and preparation method and diluting method thereof as well as preparation method of frozen semen of milk goat in thin pipe |
| CN104082276A (en) * | 2014-07-17 | 2014-10-08 | 赤峰市农牧科学研究院 | Zinc-containing sheep seminal fluid diluting storage liquid and preparation method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1475109A (en) * | 2002-08-16 | 2004-02-18 | 王光亚 | Boole goat fine tube seminal fluid freezing method |
| CN102578079A (en) * | 2012-02-08 | 2012-07-18 | 青岛德瑞骏发生物科技有限公司 | Base fluid for diluting semens of equus animals and preparation method and use method thereof |
| CN103518706A (en) * | 2013-10-21 | 2014-01-22 | 西北农林科技大学 | Diluent of frozen semen of milk goat, and preparation method and diluting method thereof as well as preparation method of frozen semen of milk goat in thin pipe |
| CN104082276A (en) * | 2014-07-17 | 2014-10-08 | 赤峰市农牧科学研究院 | Zinc-containing sheep seminal fluid diluting storage liquid and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108094409A (en) * | 2018-01-12 | 2018-06-01 | 刘铮铸 | The preparation method and applications of ram freezing semen dilution |
| CN108452290A (en) * | 2018-06-21 | 2018-08-28 | 陕西奥能生物科技有限公司 | A kind of embryo gender interference preparation of sheep, preparation method and applications |
| CN109221091A (en) * | 2018-11-01 | 2019-01-18 | 洛宁农本畜牧科技开发有限公司 | Fresh smart low temperature dilution of a kind of animal and the preparation method and application thereof |
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