CN106290826A

CN106290826A - Colour band height quantitative method

Info

Publication number: CN106290826A
Application number: CN201510266218.6A
Authority: CN
Inventors: 熊光滨
Original assignee: Individual
Current assignee: Individual
Priority date: 2015-05-22
Filing date: 2015-05-22
Publication date: 2017-01-04

Abstract

Present invention aim at providing a kind of without integrity problem, the colour band height quantitative method of the quantification and qualification problem that will not be caused by operating environment change impact.This colour band height quantitative method comprises the following steps: step one: be immersed in reagent solution by whole reagent paper, and drying forms several first test strips after cutting, then using the first test strips of whole piece as reaction zone；Step 2: the second test strips of a measured object containing concentration known is set separately, using as reference standard district；And step 3: to this reference standard district and this reaction zone, carry out the analysis of colour band height length, it may be judged whether exceed limitation standard.

Description

Colour band height quantitative method

Technical field

The present invention relates to a kind of colour band height quantitative method, espespecially one can apply to immunity-chromatography test The competition law of paper and sandwich, the biochemical biocatalyst of chromatographic test paper, chemical analysis reagent paper The quantitative method in Qualitative and quantitative analysis field.

Background technology

In twentieth century five, the sixties in response to social need, reagent paper colour generation formula the most organic And inorganic chemical analysis method has emitted out the most like the mushrooms after rain, but owing to not having Equipment coordinates accurate quantitative analysis of making comparisons, and leaves behind PH and the examination of sxemiquantitative urine now Paper, because the most widely used person of its convenience is applied.

The demand testing pregnant fast and convenient diagnostic reagent that be developed by immunochromatography technique, and create Produce field diagnostic [point-of-care testing is called for short POCT] industry, immunochromatography The application of the reagent whole world has spread to more than hundred kinds products.

And, practical agricultural beyond external clinical diagnosis, food safety detection, prudence, Even in animal health management industry.

Comparatively speaking, for the congenital restriction on the reagent paper quantitation methodology that quantitative requirement produces, The immune chromatography test paper overwhelming majority is applied to qualitative analysis, at many measured objects and quantitative square The development in face is extremely limited.

Even qualitative analysis, immunochromatography is also because being easier to by biological reagent constituent Affected by various subjective and objective factors and decayed, strictly speaking, on product stability less Easy to control.

At the scene on rapidly and quantitatively testing, owing to field fabrication standard inspection amount cannot be being used in real time Line, every correction parameter of every batch of POCT set group reagent calibration curve, is to set when dispatching from the factory , decay after therefore reagent composition dispatches from the factory, necessarily affect reagent testing result can By property, the colorrendering quality of reagent colour generation is the best, thus is the bad dream of this area practitioner.

When using immunochromatography reagent to make quantitative analysis, again because execute-in-place temperature is with relative Humidity has a strong impact on the capillarity of chromatography reagent, causes inaccurate quantitative analysis consequence.

In view of this, when how to provide one can overcome the detection paper reaction color degree of depth, faced Agent formulations decays the integrity problem that causes, and operating environment change caused qualitative with The colour band height quantitative method of quantitative analysis problem, becomes the purpose to be improved for the present invention.

Summary of the invention

The purpose of the present invention, is to provide a kind of without integrity problem, will not be changed by operating environment The colour band height quantitative method of the quantification and qualification problem affected and cause.

For solving the problems referred to above and reaching the purpose of the present invention, technical scheme is as follows:

A kind of colour band height quantitative method, it is characterised in that comprise the following steps: step I: by whole Opening reagent paper to be immersed in reagent solution, drying forms several first test strips after cutting, then with whole First test strips of bar is as reaction zone；Step II: arrange one separately containing concentration known Second test strips of measured object, using as reference standard district；And step III: to this comparison Standard regions and this reaction zone, carry out the analysis of colour band height length, it may be judged whether exceed restriction Standard.

In one embodiment of this invention, in described step III, the analysis of colour band height length Mode, is one of following manner: range estimation is compared, photochemistry reads, electrochemical methods is read Take.

In one embodiment of this invention, described first test strips and the second test strips, Liang Zhewei Arrange in the way of being parallel to each other, formed one detection device, and both respectively with liquid stream side To parallel.

In one embodiment of this invention, the reagent solution in described step I, is for one of following: Antibody working solution, antigen working solution, enzyme reagent solution, chemical reagent liquid.

In one embodiment of this invention, in described step III, analysis mode is not range estimation ratio Time relatively, also include the standard substance readings of the measured object of concentration known, be inserted in setting formula Carry out parameters revision computing, in the hope of the step of measured object actual concentrations value multiple in specimen.

Understand according to above-mentioned, the invention has the beneficial effects as follows:

First point: in the method for the present invention, discloses a kind of new chromatography quantitative analysis method, Set not with the reaction zone shade of reagent paper as foundation, but be immersed in relevant with whole reagent paper In reagent solution, drying cut after with whole piece test strips as reaction zone, simultaneously arrange one separately Containing the test strips of concentration known measured object, to use as reference standard district, apply upper ten The convenience divided.

Second point: in the method for the present invention, reacts colour generation through chromatography effect with measured object with reagent paper Band height length, as the foundation of quantification and qualification, this correcting mode on the spot with Quoting alternately of standard adding technique, overcomes detection paper reaction color degree of depth time institute completely Face agent formulations decay integrity problem, and operating environment change caused qualitative With quantitative analysis problem.

Accompanying drawing explanation

The schematic flow sheet of Fig. 1: the present invention.

The enforcement schematic diagram of Fig. 2～Fig. 4: the present invention.

Reference:

1 first test strips

2 second test strips

21 measured objects

100 detection devices

T reaction zone

S reference standard district

Detailed description of the invention

Describe in detail as follows according to the embodiment shown in drawing:

As shown in Figure 1 to 4, for a kind of colour band height quantitative method, it comprises the steps of Step I: be immersed in reagent solution by whole reagent paper, drying forms several the first examinations after cutting Paper slip 1, then using the first test strips 1 of whole piece as reaction zone T；Step II: arrange separately One the second test strips 2 of measured object 21 containing concentration known, using as reference standard district S； And step III: to this reference standard district S and this reaction zone T, carry out colour band height length Analyze, it may be judged whether exceed limitation standard.

In the colour band height quantitative method of the present invention, it is not for dividing with the shade of test strips Analysis standard, is through reaction zone T and the cooperation of reference standard district S, with reagent paper through layer on the contrary Analysis effect and measured object react the height length in colour band, depending on as quantification and qualification According to, this correcting mode on the spot is quoted alternately with standard adding technique, and especially at one stroke When overcoming the detection paper reaction color degree of depth, it is reliable that the decay of faced agent formulations causes Sex chromosome mosaicism, solves operating environment more simultaneously in the lump and changes the quantification and qualification caused Problem.

Therefore, guaranteeing of high-accuracy, with regard to the inspection not carried out at the scene at control laboratory, Its result also can quickly occur, it is not necessary to fixing, special place, can enter the most on the spot Row is checked at once, is addressed reviewer's suspection to POCT science and technology.

In above-mentioned, in described step III, the analysis mode of colour band height length, is for following side One of formula: range estimation is compared, photochemistry reads, electrochemical methods reads.

Wherein, according to measured target, the difference of tested demand, apply different analysis modes, Optimal assay can be obtained.

In above-mentioned, described first test strips 1 and the second test strips 2, both are for be parallel to each other Mode and arrange, form a detection device 100, and both are parallel with liquid stream direction respectively.

Wherein, by detecting the application of device 100, the present invention can be quickly employed, and The method to set up parallel with liquid stream direction, not only can be easy to detection, during hand-held use, especially Do not worry that measured object 21 can be infected with in one's hands.

In above-mentioned, the reagent solution in described step I, can be one of following: antibody working solution, Antigen working solution, enzyme reagent solution, chemical reagent liquid.

Wherein, by applying different reagent solutions, the present invention is provided that the test applied different On.

In above-mentioned, in described step III, analysis mode is not range estimation when comparing, also include by The standard substance readings of the measured object 21 of concentration known, is inserted in setting formula corrected parameter and transports Calculate and revise, in the hope of the step of the actual concentrations value of measured object multiple in specimen.

Wherein, by this step, can make the present invention when test, do not worry occurring because of Error and produce erroneous judgement problem occur, can quickly obtain measured object concentration value accurately.

Above-mentioned according to graphic shown embodiment, describe the structure of the present invention, feature and work in detail By effect, the foregoing is only present pre-ferred embodiments, but the present invention is not with shown in drawing Limit practical range, the most all with it is intended that the modified that is consistent changes, as long as waiting All should be covered by the present invention program protection domain in the range of effect.

Claims

1. a colour band height quantitative method, it is characterised in that comprise the following steps:

Step (I): being immersed in reagent solution by whole reagent paper, drying forms several after cutting First test strips (1), then using first test strips (1) of whole piece as reaction zone (T)；

Step (II): the second reagent paper of a measured object (21) containing concentration known is set separately Bar (2), using as reference standard district (S)；And

Step (III): to this reference standard district (S) and this reaction zone (T), carries out colour band height The analysis of length, it may be judged whether exceed limitation standard.

2. colour band height quantitative method as claimed in claim 1, it is characterised in that: described step Suddenly in (III), the analysis mode of colour band height length, for one of following manner: range estimation compares, Photochemistry reads, electrochemical methods reads.

3. colour band height quantitative method as claimed in claim 1, it is characterised in that: described the One test strips (1) and the second test strips (2), both are arranged in the way of being parallel to each other, shape Become detection device (100), and both are parallel with liquid stream direction respectively.

4. colour band height quantitative method as claimed in claim 1, it is characterised in that: described step Suddenly the reagent solution in (I), for one of following: antibody working solution, antigen working solution, enzyme try Agent liquid, chemical reagent liquid.

5. colour band height quantitative method as claimed in claim 2, it is characterised in that: described step Suddenly in (III), analysis mode is not range estimation when comparing, and also include concentration known is tested The standard substance readings of thing (21), is inserted in setting formula and carries out parameters revision computing, in the hope of mark The step of the actual concentrations value of multiple measured object in Ben.