CN106290755A - Measure the method for alpha-glucan content in sugarcane juice - Google Patents
Measure the method for alpha-glucan content in sugarcane juice Download PDFInfo
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- CN106290755A CN106290755A CN201610775941.1A CN201610775941A CN106290755A CN 106290755 A CN106290755 A CN 106290755A CN 201610775941 A CN201610775941 A CN 201610775941A CN 106290755 A CN106290755 A CN 106290755A
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- sugarcane juice
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/14—Beverages
- G01N33/143—Beverages containing sugar
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Abstract
The present invention relates to sugar production field, be specifically related to measure the method for alpha-glucan content in sugarcane juice, sugarcane juice adds amylase and alpha-glucan enzyme, the alpha-glucan concentration obtained and the relation curve of turbidity；Sugarcane juice to be measured adds amylase, fully reacts, and sugarcane juice, the volume ratio of both amylase are 2000:1；It is sufficiently mixed and reacts；It is the oxalic acid of 5% that reaction adds concentration after terminating, the addition of oxalic acid be sugarcane juice volume ratio be 0.003%, filter after mix homogeneously, add ethanol, ethanol and sugarcane juice filtrate volume are 1:1, measure the turbidity value of filtrate；Contrast with the relation curve of alpha-glucan concentration and turbidity, obtain sugarcane juice alpha-glucan concentration to be measured.What the present invention provided measures the method for alpha-glucan content in sugarcane juice, and simple to operate, it is not necessary to special equipment, monitoring velocity is fast.
Description
Technical field
The present invention relates to sugar production field, be specifically related to measure the method for alpha-glucans content in sugarcane juice.
Background technology
When the alpha-glucans content in sugarcane juice reaches to a certain degree, its high viscosity can cause sugarcane juice to filter, concentrate, crystallize
Deng subsequent operation difficulty, also result in a large amount of losses of sugar simultaneously.Therefore, the alpha-glucans content in quantitative analysis sugarcane juice has
Highly important realistic meaning.The assay method of alpha-glucans content in sugarcane juice, mainly has Robert's copper method, polarimetry, enzymolysis
Chromatography, antibody act etc..Robert's copper method needs cyclic washing, multiple times of filtration, and operating procedure is too much, and resultant error is big；Polarimetry
Special instrument is needed with enzymolysis chromatography determination.
Summary of the invention
The present invention provides a kind of method of alpha-glucans content in simple mensuration sugarcane juice.
The technical solution adopted in the present invention is:
Measure the method for alpha-glucans content in sugarcane juice, comprise the following steps:
(1) in sugarcane juice, amylase and alpha-glucanase, sugarcane juice, amylase, the volume ratio of alpha-glucanase three are added
For 2000:1:1；It is sufficiently mixed and reacts；It is the oxalic acid of 5% that reaction adds concentration after terminating, and the addition of oxalic acid is sugarcane juice body
Long-pending ratio is 0.003%, filters after mix homogeneously, and the blank sugarcane juice filtrate obtained, blank sugarcane juice filtrate adds second
Alcohol, ethanol and blank sugarcane juice filtrate volume are 1:1；Blank sugarcane juice filtrate is divided into many parts, is separately added into different amounts of
Alpha-glucans solution so that the content of the alpha-glucans in each part blank sugarcane juice filtrate changes in gradient；Measure every a
The turbidity of blank sugarcane juice filtrate, the alpha-glucans concentration obtained and the relation curve of turbidity；
(2) sugarcane juice to be measured adds amylase, fully reacts, and sugarcane juice, the volume ratio of both amylase are 2000:1；The most mixed
Merge reaction；Reaction terminate after add concentration be the oxalic acid of 5%, the addition of oxalic acid be sugarcane juice volume ratio be 0.003%, mixing
Filtering after uniformly, add ethanol, ethanol and sugarcane juice filtrate volume are 1:1, measure the turbidity value of filtrate；
(3) relation curve with alpha-glucans concentration and turbidity contrasts, and obtains sugarcane juice alpha-glucans concentration to be measured.
What the present invention provided measures the method for alpha-glucans content in sugarcane juice, simple to operate, it is not necessary to special equipment, prison
Degree of testing the speed is fast.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated, but the present invention is not limited only to these embodiments, not
On the premise of departing from present inventive concept, within any improvement made all falls within protection scope of the present invention.
Measure the method for alpha-glucans content in sugarcane juice, comprise the following steps:
(1) in sugarcane juice, amylase and alpha-glucanase, sugarcane juice, amylase, the volume ratio of alpha-glucanase three are added
For 2000:1:1；It is sufficiently mixed and reacts；It is the oxalic acid of 5% that reaction adds concentration after terminating, and the addition of oxalic acid is sugarcane juice body
Long-pending ratio is 0.003%, filters after mix homogeneously, and the blank sugarcane juice filtrate obtained, blank sugarcane juice filtrate adds second
Alcohol, ethanol and blank sugarcane juice filtrate volume are 1:1；Blank sugarcane juice filtrate is divided into many parts, is separately added into different amounts of
Alpha-glucans solution so that the content of the alpha-glucans in each part blank sugarcane juice filtrate changes in gradient；Measure every a
The turbidity of blank sugarcane juice filtrate, the alpha-glucans concentration obtained and the relation curve of turbidity；
(2) sugarcane juice to be measured adds amylase, fully reacts, and sugarcane juice, the volume ratio of both amylase are 2000:1；The most mixed
Merge reaction；Reaction terminate after add concentration be the oxalic acid of 5%, the addition of oxalic acid be sugarcane juice volume ratio be 0.003%, mixing
Filtering after uniformly, add ethanol, ethanol and sugarcane juice filtrate volume are 1:1, measure the turbidity value of filtrate；
(3) relation curve with alpha-glucans concentration and turbidity contrasts, and obtains sugarcane juice alpha-glucans concentration to be measured.
Claims (1)
1. measure the method for alpha-glucans content in sugarcane juice, it is characterised in that: comprise the following steps:
(1) adding amylase and alpha-glucanase in sugarcane juice, sugarcane juice, amylase, the volume ratio of alpha-glucanase three are
2000:1:1；It is sufficiently mixed and reacts；It is the oxalic acid of 5% that reaction adds concentration after terminating, and the addition of oxalic acid is sugarcane juice volume ratio
Being 0.003%, filter after mix homogeneously, the blank sugarcane juice filtrate obtained, blank sugarcane juice filtrate adds ethanol, second
Alcohol and blank sugarcane juice filtrate volume are 1:1；Blank sugarcane juice filtrate is divided into many parts, is separately added into different amounts of α-Portugal and gathers
Sugar juice so that the content of the alpha-glucans in each part blank sugarcane juice filtrate changes in gradient；It is every a blank right to measure
According to the turbidity of sugarcane juice filtrate, the alpha-glucans concentration obtained and the relation curve of turbidity；
(2) sugarcane juice to be measured adds amylase, fully reacts, and sugarcane juice, the volume ratio of both amylase are 2000:1；It is sufficiently mixed also
Reaction；Reaction terminate after add concentration be the oxalic acid of 5%, the addition of oxalic acid be sugarcane juice volume ratio be 0.003%, after mix homogeneously
Filtering, add ethanol, ethanol and sugarcane juice filtrate volume are 1:1, measure the turbidity value of filtrate；
(3) relation curve with alpha-glucans concentration and turbidity contrasts, and obtains sugarcane juice alpha-glucans concentration to be measured.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610775941.1A CN106290755A (en) | 2016-08-31 | 2016-08-31 | Measure the method for alpha-glucan content in sugarcane juice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610775941.1A CN106290755A (en) | 2016-08-31 | 2016-08-31 | Measure the method for alpha-glucan content in sugarcane juice |
Publications (1)
| Publication Number | Publication Date |
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| CN106290755A true CN106290755A (en) | 2017-01-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610775941.1A Pending CN106290755A (en) | 2016-08-31 | 2016-08-31 | Measure the method for alpha-glucan content in sugarcane juice |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110609245A (en) * | 2019-10-09 | 2019-12-24 | 广西思晔自动化科技有限公司 | Sugarcane amount micro-magnetic measurement control method and device |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102353639A (en) * | 2011-07-07 | 2012-02-15 | 广州甘蔗糖业研究所 | Method for determining starch content of cane juice or syrup |
| CN102590210A (en) * | 2012-02-21 | 2012-07-18 | 广州甘蔗糖业研究所 | Method for measuring content of alpha-glucosan in cane juice |
-
2016
- 2016-08-31 CN CN201610775941.1A patent/CN106290755A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102353639A (en) * | 2011-07-07 | 2012-02-15 | 广州甘蔗糖业研究所 | Method for determining starch content of cane juice or syrup |
| CN102590210A (en) * | 2012-02-21 | 2012-07-18 | 广州甘蔗糖业研究所 | Method for measuring content of alpha-glucosan in cane juice |
Non-Patent Citations (3)
| Title |
|---|
| 班甲等: "酒精Haze改良法测定蔗汁α-葡聚糖含量", 《食品科技》 * |
| 胡玉玫等: "糖料甘蔗品质评定新指标一甘蔗葡聚糖的形成、危害及检测方法", 《甘蔗糖业》 * |
| 蚁细苗等: "免疫比浊法定量测定制糖过程中的葡聚糖", 《广西蔗糖》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110609245A (en) * | 2019-10-09 | 2019-12-24 | 广西思晔自动化科技有限公司 | Sugarcane amount micro-magnetic measurement control method and device |
| CN110609245B (en) * | 2019-10-09 | 2024-02-27 | 广西思晔自动化科技有限公司 | Micro-magnetic measurement control method and device for sugarcane squeezing quantity |
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Application publication date: 20170104 |
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