CN106290736A - A kind of small polypide micro-dosage pin type leaching medicine method of testing and device thereof - Google Patents
A kind of small polypide micro-dosage pin type leaching medicine method of testing and device thereof Download PDFInfo
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Abstract
The present invention relates to a kind of small polypide micro-dosage pin type leaching medicine method of testing and device thereof.The method uses the small polypide of the present invention micro-dosage pin type leaching medicine test device to carry out.This device is become with apparatus for adhesion-catching insects by base, and base is microscope slide and supports seat, and seat is fixed on microscope slide, its end face is provided with groove in support；Apparatus for adhesion-catching insects is made up of mythimna separata pin, connecting rod, protection pipe and handle; transparent, a diameter of 0.5～1 millimeter of mythimna separata pin; the surface parcel layer of transparent adhesive of mythimna separata pin; connecting rod two ends are connected with mythimna separata pin and handle respectively; the length of connecting rod is longer 2 centimetres than the length of protection pipe; a length of 1～1.5 centimetre of mythimna separata pin, mythimna separata pin and part connecting rod insert in protection is managed and connecting rod is threadeded with protection pipe, and protection pipe is placed in the groove supporting seat.Due to the fact that the surface area of mythimna separata pin is little, medicament usage amount can be reduced, reduce experimentation cost, meet the required polypide of the Research Requirements of micro constitutent, precisely selection, be prone to observe counting.
Description
Technical field
The invention belongs to agricultural small polypide indoor medicament testing research technical field, particularly relate to natural product active testing
Technical field.
Background technology
For agriculture and forestry injurious insect, especially as phytophagous mites, the Aphidoidea of Tetranychidae (Tetranychidae)
(Aphidoidea) indoor of the micro pests (0.1～1 millimeter) such as Phytophthira, the eliminating coccids-line of Coccoidea (Coccoidea)
Pharmacy test, frequently with method have foliage-spray method, blade medicine embrane method, slide infusion process etc., all there is consumption in these methods
The shortcomings such as substantial amounts of medicament, external interference factor is many, statistical observation is relatively difficult, test result error is bigger.And for medicament
Much more expensive or that amount is the fewest situation, these methods are the most inapplicable.Such as the secondary metabolite at plant source or microbial source
In insecticidal activity research, reactive compound often only several milligrams, it is difficult to complete pharmacy test.The method of testing of the most micro-dosage
Being particularly important, micro-dosage pin type of the present invention leaching medicine method of testing can well solve these problems.
Summary of the invention
For meeting dosing observation few, easy during the pharmacy test of agricultural micro pest indoor, workable
Etc. demand, the present invention provides a kind of small polypide micro-dosage leaching medicine method of testing and device thereof.
Technical scheme is as follows:
1. small polypide micro-dosage pin type leaching medicine test device, this device is made up of base and apparatus for adhesion-catching insects, the described end
Seat is made up of microscope slide and support seat, and the end face supporting seat is provided with groove, and the upper surface of the bottom surface and microscope slide that support seat is fixed
Connect；Described apparatus for adhesion-catching insects is made up of mythimna separata pin, connecting rod, protection pipe and handle, and described mythimna separata pin is transparent, and mythimna separata pin
A diameter of 0.5～1 millimeter, and it is coated with layer of transparent adhesive at mythimna separata pin, one end of mythimna separata pin and connecting rod
One end is fixing to be connected, and the other end of connecting rod is fixing with one end of handle to be connected, and the outer wall of connecting rod is provided with external screw thread, protection pipe
Inwall be provided with the female thread that the external screw thread with connecting rod outer wall matches, the length of connecting rod is longer 2 lis than the length of protection pipe
Connection in rice, a length of 1～1.5 centimetre of mythimna separata pin, mythimna separata pin and part connecting rod insert protection pipe and in protection pipe
Bar is threaded connection with protection pipe, and protection pipe is placed in the groove supporting seat.
2. small polypide micro-dosage pin type leaching medicine method of testing, the method uses the one described in technical scheme 1 micro-
Small polypide is carried out soaking medicine test by microzooid micro-dosage pin type leaching medicine test device, comprises the following steps:
A, taking off apparatus for adhesion-catching insects from base, rotary handle makes mythimna separata pin stretch out in protection pipe；
B, selecting polypide under the auxiliary of magnifier, hand-held handle makes the polypide back selected by the adhesion of mythimna separata pin, will be viscous
The mythimna separata pin having polypide inserts in miniature ampoule, and leaching medicine 3～hand-held handle taking-up mythimna separata pin after 5 seconds, then by apparatus for adhesion-catching insects
Put back in the groove of bracing frame, and whole small polypide micro-dosage pin type leaching medicine test device is placed under Stereo microscope sight
Examine, the activity of record different time sections polypide and death condition.
3, according to described in technical scheme 3 the small polypide of one micro-dosage pin type soak medicine method of testing, described in step B
Selecting polypide under the auxiliary of magnifier, its described magnifier is 20 times of magnifieres.
4, medicine method of testing, institute in step B are soaked according to the small polypide of the one described in technical scheme 2 or 3 micro-dosage pin type
The miniature ampoule stated is 1～1.5 milliliter of centrifuge tube.
Compared with prior art, the invention has the beneficial effects as follows:
1, experimentation cost is reduced: existing slide infusion process is patch double faced adhesive tape at the 1/3 of microscope slide one end, by blade
Polypide with hairbrush brush on blank sheet of paper, under magnifier, then select polypide, post the part of double faced adhesive tape with slide viscous selected
Polypide, is partially placed in ampoule leaching medicine certain time subsequently by the slide being stained with polypide, and it is micro-that rear taking-up slide is placed in body formula
Microscopic observation, the activity of record different time sections polypide and death condition.Owing to slide is wider, during leaching medicine, the medicament of needs is relatively
Many, typically at least want the medicament of 5 milliliters, on the other hand, when gluing selected polypide with slide, owing to fragmentation is wider, the most easily
Other polypides unwanted are adhered, other impurity of even adhering, have impact on the observation to selected polypide, thus affect observation number
According to accuracy.And due to the fact that the mythimna separata pin using a diameter of 0.5～1 millimeter, so consumption pharmaceutical quantities greatly reduces, generally
Only needing 0.5-1mL medicament, therefore, because of the present invention, to consume the dosage of medicament little, and the test expensive for medicament can be substantially reduced
Experimentation cost.
2, the Research Requirements of micro constitutent is met: as the secondary metabolite insecticidal activity at plant source or microbial source is ground
In studying carefully, reactive compound often only several milligrams, it is difficult to complete routine such as foliage-spray method, blade medicine embrane method, slide impregnates
The insecticidal activity tests such as method.And drug dose needed for the small polypide of one of the present invention micro-dosage pin type leaching medicine method of testing
Little, can be few with meet volume and without commercially available compound insecticidal activity research.
3, required polypide is precisely selected: because the surface area little (diameter 0.5～1 millimeter) of mythimna separata pin, can be at magnifier
Auxiliary under precisely cling selected object without adhere to that neighbouring other polypides, residuum or interference observe other are miscellaneous
Matter.
4, it is prone to observe: because mythimna separata pin diameter little (0.5～1 millimeter), be easy to allow polypide fitly be arranged in mythimna separata
On pin, and mythimna separata pin is transparent, it is easy to observe counting.
5, the small polypide of the present invention micro-dosage pin type leaching medicine test apparatus structure is ingenious, can solve existing method and consume a large amount of
Medicament, the shortcoming such as many by external interference factor, statistical observation is relatively difficult, test result error is bigger, its protection pipe be used for
Protection mythimna separata pin is not contaminated, and not by external force deformation, can have longer service life by ensuring equipment.The structure letter of apparatus of the present invention
Singly, remove and install conveniently, simple to operate, it is easy to manufacture, low cost of manufacture, it is easy to promote.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention a kind of small polypide micro-dosage pin type leaching medicine test device.
Fig. 2 is the structural representation of apparatus for adhesion-catching insects.
Fig. 3 is the structural representation that mythimna separata pin, connecting rod and handle connect.
Fig. 4 is the structural representation of base.
In figure, each labelling represents successively: 1 microscope slide, and 2 support seat, 3 handles, and 4 protections are managed, 5 connecting rods,
6 mythimna separata pins, 7 grooves.
Detailed description of the invention
Embodiment 1
See Fig. 1-Fig. 4, the small polypide of one provided by the present invention micro-dosage pin type leaching medicine test device by base and
Apparatus for adhesion-catching insects is constituted, and described base is made up of microscope slide 1 and support seat 2, and the end face supporting seat 2 is provided with groove 7, supports the end of seat 2
Face is fixing with the upper surface of microscope slide 1 to be connected；Described apparatus for adhesion-catching insects is made up of mythimna separata pin 6, connecting rod 5, protection pipe 4 and handle 3, institute
State mythimna separata pin 6 for transparent, and a diameter of 0.5 millimeter or 1 millimeter of mythimna separata pin 6, and be enclosed with one at the outer surface of mythimna separata pin 6
Layer transparent adhesive, transparent adhesive is commercially available prod, and one end of mythimna separata pin 6 is fixing with one end of connecting rod 5 to be connected, connecting rod
The other end of 5 is fixing with one end of handle 3 to be connected, and the outer wall of connecting rod 5 is provided with external screw thread, and the inwall of protection pipe 4 is provided with and connects
The female thread that the external screw thread of extension bar 5 outer wall matches, the length of connecting rod 5 is longer 2 centimetres than the length of protection pipe 4, mythimna separata pin 6
A length of 1 centimetre or 1.5 centimetres, the connection in mythimna separata pin 6 total length and part connecting rod 5 insert protection pipe 4 and in protection pipe 4
Bar 5 is threaded connection with protection pipe 4, and in protection pipe 4 is placed on the groove 7 supporting seat 2, i.e. whole apparatus for adhesion-catching insects is placed on support
In the groove 7 of seat 2.When not using the small polypide of the present invention micro-dosage pin type leaching medicine test device, make to glue by rotary handle 3
Worm pin 6 total length is in protection pipe 4, to protect mythimna separata pin 6 not to be contaminated, not by external force deformation.
Embodiment 2
The small polypide of one provided by the present invention micro-dosage pin type leaching medicine method of testing, uses described in embodiment 1
Plant small polypide micro-dosage pin type leaching medicine test device to carry out small polypide soaking medicine test, comprise the following steps:
A, bracing frame 2 from base groove 7 in take off apparatus for adhesion-catching insects, rotary handle 3 makes mythimna separata pin 6 in protection pipe 4
Stretch out；
B, under the auxiliary of 20 times of common magnifying glass according to test requirements document select polypide, hand-held handle 3 makes mythimna separata pin 6 adhere
Selected polypide back, inserts in miniature ampoule by the mythimna separata pin 6 being stained with polypide, and miniature ampoule can be with 1～1.5 milliliter
Centrifuge tube replaces, leaching medicine 3～after 5 seconds, and hand-held handle 3 takes out mythimna separata pin 6, and that is then put back to by apparatus for adhesion-catching insects at bracing frame 2 is recessed
In groove 7, and whole small polypide micro-dosage pin type leaching medicine test device is placed under Stereo microscope observation, records different time
The activity of section polypide and death condition.Being coated with layer of transparent adhesive due to mythimna separata pin 6, therefore, mythimna separata pin 6 holds very much
The easily polypide selected by adhesion, the time of the medicine soaked and leaching medicine determines according to a conventional method according to different types of small polypide.
Claims (4)
1. small polypide micro-dosage pin type leaching medicine test device, it is characterised in that: this device is made up of base and apparatus for adhesion-catching insects,
Described base is made up of microscope slide (1) and support seat (2), and the end face supporting seat (2) is provided with groove (7), supports the bottom surface of seat (2)
Fix with the upper surface of microscope slide (1) and be connected；Described apparatus for adhesion-catching insects is by mythimna separata pin (6), connecting rod (5), protection pipe (4) and handle
(3) composition, described mythimna separata pin (6) is transparent, and a diameter of 0.5～1 millimeter of mythimna separata pin (6), and at the table of mythimna separata pin (6)
Bread is wrapped with layer of transparent adhesive, and one end of mythimna separata pin (6) is fixing with one end of connecting rod (5) to be connected, connecting rod (5) another
One end is fixing with one end of handle (3) to be connected, and the outer wall of connecting rod (5) is provided with external screw thread, and the inwall of protection pipe (4) is provided with and connects
The female thread that the external screw thread of extension bar (5) outer wall matches, the length of connecting rod (5) is than the length length 2 centimetres of protection pipe (4), viscous
In a length of 1～1.5 centimetre of worm pin (6), mythimna separata pin (6) and part connecting rod (5) insert protection pipe (4) and at protection pipe
(4) connecting rod (5) in is threaded connection with protection pipe (4), and protection pipe (4) is placed in the groove (7) supporting seat (2).
2. small polypide micro-dosage pin type leaching medicine method of testing, it is characterised in that: the method uses described in claim 1
Small polypide is carried out soaking medicine test by a kind of small polypide micro-dosage pin type leaching medicine test device, comprises the following steps:
A, taking off apparatus for adhesion-catching insects from base, rotary handle (3) makes mythimna separata pin (6) stretch out in protection pipe (4)；
B, under the auxiliary of magnifier select polypide, hand-held handle (3) make mythimna separata pin (6) adhesion selected by polypide back, will
The mythimna separata pin (6) being stained with polypide inserts in miniature ampoule, leaching medicine 3～hand-held handle (3) taking-up mythimna separata pin (6) after 5 seconds, so
After apparatus for adhesion-catching insects is placed in the groove (7) of bracing frame (2), and whole small polypide micro-dosage pin type leaching medicine test device is placed in
Observe under Stereo microscope, the activity of record different time sections polypide and death condition.
The small polypide of one the most according to claim 2 micro-dosage pin type leaching medicine method of testing, it is characterised in that: step B
Described under the auxiliary of magnifier select polypide, its described magnifier is 20 times of magnifieres.
4. soak medicine method of testing according to the small polypide of the one described in Claims 2 or 3 micro-dosage pin type, it is characterised in that: step
Miniature ampoule described in rapid B is 1～1.5 milliliter of centrifuge tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610756939.XA CN106290736B (en) | 2016-08-29 | 2016-08-29 | A kind of micro- dosage pin type of small polypide soaks medicine test method and its device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610756939.XA CN106290736B (en) | 2016-08-29 | 2016-08-29 | A kind of micro- dosage pin type of small polypide soaks medicine test method and its device |
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| Publication Number | Publication Date |
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| CN106290736A true CN106290736A (en) | 2017-01-04 |
| CN106290736B CN106290736B (en) | 2018-06-15 |
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| CN201610756939.XA Active CN106290736B (en) | 2016-08-29 | 2016-08-29 | A kind of micro- dosage pin type of small polypide soaks medicine test method and its device |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN88200515U (en) * | 1988-01-27 | 1988-12-21 | 中国农业科学院养蜂研究所 | Pen type insect remover with spring inside |
| CN201839726U (en) * | 2010-08-04 | 2011-05-25 | 赵学民 | Environmentally-friendly worm sticking rope |
| CN202151386U (en) * | 2011-07-26 | 2012-02-29 | 河北农业大学 | Tweezers provided with rubber noses and used for clamping living insects |
| CN203275387U (en) * | 2013-06-06 | 2013-11-06 | 西南大学 | Fast biologic assay device for fruit fly pest larva |
| CN203860090U (en) * | 2014-04-29 | 2014-10-08 | 高锦乐 | Acupuncture infection device for artificially culturing caterpillar fungi |
| CN104483266A (en) * | 2015-01-05 | 2015-04-01 | 福建农林大学 | Simple device and measuring method for measuring adhesive force of small insects and liquid |
-
2016
- 2016-08-29 CN CN201610756939.XA patent/CN106290736B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN88200515U (en) * | 1988-01-27 | 1988-12-21 | 中国农业科学院养蜂研究所 | Pen type insect remover with spring inside |
| CN201839726U (en) * | 2010-08-04 | 2011-05-25 | 赵学民 | Environmentally-friendly worm sticking rope |
| CN202151386U (en) * | 2011-07-26 | 2012-02-29 | 河北农业大学 | Tweezers provided with rubber noses and used for clamping living insects |
| CN203275387U (en) * | 2013-06-06 | 2013-11-06 | 西南大学 | Fast biologic assay device for fruit fly pest larva |
| CN203860090U (en) * | 2014-04-29 | 2014-10-08 | 高锦乐 | Acupuncture infection device for artificially culturing caterpillar fungi |
| CN104483266A (en) * | 2015-01-05 | 2015-04-01 | 福建农林大学 | Simple device and measuring method for measuring adhesive force of small insects and liquid |
Non-Patent Citations (1)
| Title |
|---|
| 陈年春: "《农药生物测定技术》", 31 October 1991, 北京农业大学出版社 * |
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| CN106290736B (en) | 2018-06-15 |
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