CN106290631B - A kind of method of pigment array sensing technology Fast Evaluation magma Quality of Liquors - Google Patents
A kind of method of pigment array sensing technology Fast Evaluation magma Quality of Liquors Download PDFInfo
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- CN106290631B CN106290631B CN201610632326.5A CN201610632326A CN106290631B CN 106290631 B CN106290631 B CN 106290631B CN 201610632326 A CN201610632326 A CN 201610632326A CN 106290631 B CN106290631 B CN 106290631B
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Abstract
The invention discloses a kind of methods of pigment array sensing technology Fast Evaluation magma Quality of Liquors, include the following steps:(1)Two or more air-sensitive pigment is chosen, pigment sensor array is integrated;(2)Establish the standard finger-print of different brackets original plasm wine;(3)The foundation of original plasm wine grade identification model;(4)The identification of original plasm wine grade to be measured.The present invention detects the corresponding response signal of ethyl alcohol and ethyl hexanoate in original plasm wine using pigment sensor array, the quality of its original plasm wine is evaluated by the content of analysis ethyl alcohol and ethyl acetate, the quick difference for realizing original plasm wine grade, provides guidance for liquor production.
Description
Technical field
The present invention relates to original plasm wine detection fields, and in particular to a kind of pigment array sensing technology Fast Evaluation magma vinosity
The method of amount.
Background technique
The history of China white wine brewing is quite long, can trace back to B.C. 4000-2000, wine-making technology exquisiteness,
Skill is of extensive knowledge and profound scholarship, and product style is unique, and wine body is mellow, long times of aftertaste, enjoys high reputation in the whole world.Therefore, brewed spirit industry
The mainstay industry for becoming China's food service industry has very important status in the national economic development, and becomes country
One of important sources of revenue tax.
Currently, the production technology of liquor industry still relies primarily on traditional means, amount quality picking wine in wine link, the step are steamed
Be mainly characterized by distinguish the ingredient difference of the original plasm wine distilled, judge the quality for distinguishing wine, the at present magma of brewery
Wine goes out wine process and is divided into foreshot, level-one wine, second level wine, liquor tailing four-stage, and wherein foreshot and liquor tailing are not meet subsequent technique
It is required that wine, level-one wine grade highest, second level wine grade is lower, and the price of level-one wine is ten times of second level wine or more.
In most of liquor-making enterprise, the mode manually to savour the wine is generallyd use to carry out quality grading to original plasm wine in real time,
But there is the features such as worker's experience that relies on, be easy error, be big to human body injury in this method.It will lead in this way
The problems such as standard disunity, wine product reduce, heavy economic losses is brought to brewery.To solve the problems, such as this link, large-scale brewery
It has begun and has carried out a large amount of work in terms of original plasm wine, the ingredient being currently known is more than 70 kinds of substances, is led to
Many experiments and experience comparison discovery are crossed, ethyl alcohol and ethyl acetate are most important two kinds of marker substances of original plasm wine quality.But
Current analysis means can not all be applied and carry out on-line checking in production equipment, can not distinguish in real time to the grade of wine.
With the development of instrumental science and calculation method, many new measurement methods are used in concentration of alcohol detection,
Such as near infrared spectroscopy, gas chromatography, Refractive Index Method, liquid chromatography, fluorescence method, Raman spectroscopy.But these instrument
Device equipment costly, and is difficult to realize the on-site test in workshop.
It is by high forces such as covalent bond, ionic bond and oxygen keys that pigment sensor array, which is using pigment molecular and gas,
In conjunction with, have compared with the metal-oxide gas transducers such as traditional electronic nose not vulnerable to vapor, soda acid etc. in environment
The influence of disturbing factor is a kind of ideal tools of original plasm wine Quality Detection.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of pigment array sensing technology Fast Evaluation magma Quality of Liquors
Method, using the corresponding response signal of ethyl alcohol and ethyl hexanoate in pigment sensor array detection original plasm wine, by analyzing second
The content of pure and mild ethyl acetate evaluates the quality of its original plasm wine, realizes the quick difference of original plasm wine grade, raw for white wine
It produces and guidance is provided.
The invention is realized by the following technical scheme:
A kind of method of pigment array sensing technology Fast Evaluation magma Quality of Liquors, includes the following steps:
(1)Integrated pigment sensor array:
Choose two or more air-sensitive pigment, according to Authorization Notice No. be 101074931 B of CN disclosed in the quick gas of color
The production method of body sensor array makes pigment sensor array;
(2)Establish the standard finger-print of different brackets original plasm wine:
A:Different workshop different batches original plasm wines are chosen, include foreshot, level-one wine, second level per a batch of original plasm wine
Wine, four class of liquor tailing take multiple samples for the different grades of original plasm wine of each batch respectively, measure each sample using national standard method
The concentration of this ethyl alcohol and ethyl hexanoate;
B:Each sample takes 1 ~ 5ml original plasm wine to be put into specimen bottle, is placed in headspace gas generating device, pigment sensor
Array is placed at the top of specimen bottle, after sample head space contacts 2 ~ 10min with pigment sensor array, obtains different brackets original plasm wine
The image of corresponding pigment array obtains pigment array standard finger-print by signal extraction;
(3)The foundation of original plasm wine grade identification model:
A:Using ethyl alcohol known to original plasm wine and ethyl hexanoate concentration as output valveY, different brackets original plasm wine is corresponding
Pigment sensor array finger-print data-signal is as input variableX i, obtain the quantitative model of ethyl alcohol and ethyl hexanoate concentrationY It is quantitative=F(X i);
B:Become the corresponding pigment sensor array finger-print data-signal of known different brackets original plasm wine as input
AmountX i, original plasm wine grade is as output valveZ, obtain the qualitative forecasting model of original plasm wine gradeZ Grade identifies=G(X i);
(4)The identification of original plasm wine grade to be measured:
A:Quantitative detection is placed in headspace gas and fills as a result, 1 ~ 5mL original plasm wine sample to be measured is taken to be put into specimen bottle
It sets, pigment sensor array is placed in sample to be tested top of bottle, sample head space and 2 ~ 10min of pigment sensor array haptoreaction
Afterwards, original plasm wine sample map to be measured is obtained, the finger-print data-signal of original plasm wine sample to be measured is entered into step(3)The original of foundation
Starch the quantitative model of wine ethyl alcohol and ethyl hexanoate concentrationY It is quantitative=F(X i), it is dense to predict ethyl alcohol and ethyl hexanoate in original plasm wine to be measured
Degree;
B:Qualitative detection is as a result, according to ethyl alcohol in original plasm wine and ethyl hexanoate concentration, by sample to be tested spectrum data signal
Bring step into(3)In qualitative forecasting modelZ Grade identifies=G(X i) in, identify the grade of original plasm wine.
The present invention be further improved scheme be, air-sensitive pigment selected by pigment sensor array be Bromocresol Green,
Bromothymol Blue、Thymol Blue、Cresol Red、Bromocresol Purple、Neutral Red、
5,10,15,20-tetraphenyl-21H,23H-porphin、
2,3,7,8,12,13,17,18-Octaethyl-21H,23H-porphine manganese (III)
chloride、
5,10,15,20-Tetraphenyl-21H,23H-porphine manganese (III) chloride、
5,10,15,20-Tetraphenyl-21H,23H-porphin; 5,10,15,20-Tetraphenyl-21H,
23H-porphine copper (II)、
5,10,15,20-Tetraphenyl-21H,23H-porphine iron (III) chloride;、
5,10,15,20-Tetrakis(4-methoxyphenyl)-21H,23H-porphine iron (III)
chloride;、
5,10,15,20-Tetraphenyl-21H,23H-porphine zinc、
5,10,15,20-Tetrakis(4-methoxyhenyl)-21H,23H-porphine cobalt (II)、
5,10,15,20-Tetrakis(pentafluorophenyl)-21H,23H-porphyrin iron (III)
In chloride any two or it is two or more.
Compared with prior art, the present invention having following obvious advantage:
Gas sensor is after being contacted according to sensor with gas, and sensor generates variation physically or chemically quantitatively to determine
Property analysis gas.Gas sensor technology is widely used to the multiple fields such as environment, food, medicine, but also useless at home
Gas sensor technology is used for the related patents of microorganism detection and identification.The present invention provides a kind of utilization pigment gas sensing
Technology quickly detects ethyl hexanoate and concentration of alcohol, the method for identifying original plasm wine grade in original plasm wine, establishes model, realizes to be measured
The quick differentiation of original plasm wine grade, this can provide guidance for liquor production.
Specific embodiment
Below with reference to embodiment, the present invention will be described in detail.But these embodiments are not intended to limit the present invention,
Structure that those skilled in the art are made according to these embodiments, method or transformation functionally are all contained in
In protection scope of the present invention.
By taking the original plasm wine grade for identifying distilled spirit with sesame flavour as an example, a specific embodiment of the invention is elaborated.
A kind of method of pigment array sensing technology Fast Evaluation magma Quality of Liquors includes 4 steps:
Step(1)Integrated pigment sensor array:It chooses two or more air-sensitive pigment and integrates a sensor array
It arranges, side disclosed in the production method of the preparation referenced patent CN 101074931A color-sensitive gas sensor array of sensor array
Method, pigment array obtained are placed in nitrogen atmosphere after dry 5min and obtain pigment sensor array image using camera, lead to
It crosses signal extraction and obtains blank pigment array standard diagram.
Air-sensitive pigment selects pH indicator, and porphyrin, metalloporphyrin, phthalocyanines color developing agent are one such or several.
It repeats the above method and prepares multiple identical sensor array composition test group sensor arrays, test group is sensed
Device array reacts with ethyl acetate and ethyl alcohol respectively, extracts the signal of sensor array, and it is sensitive to select response
Air-sensitive pigment is prepared into new sensor array, these new sensor arrays are used for detecting sensor array of the present invention.
The air-sensitive pigment that finishing screen is selected is 6 kinds of pH indicator:Bromocresol Green, Bromothymol
Blue,Thymol Blue,Cresol Red,Bromocresol Purple,Neutral Red;
9 kinds of Porphyrin colour reagents:
5,10,15,20-tetraphenyl-21H,23H-porphin、
2,3,7,8,12,13,17,18-Octaethyl-21H,23H-porphine manganese (III)
chloride、
5,10,15,20-Tetraphenyl-21H,23H-porphine manganese (III) chloride、
5,10,15,20-Tetraphenyl-21H,23H-porphin; 5,10,15,20-Tetraphenyl-21H,
23H-porphine copper (II)、
5,10,15,20-Tetraphenyl-21H,23H-porphine iron (III) chloride;、
5,10,15,20-Tetrakis(4-methoxyphenyl)-21H,23H-porphine iron (III)
chloride;、
5,10,15,20-Tetraphenyl-21H,23H-porphine zinc、
5,10,15,20-Tetrakis(4-methoxyhenyl)-21H,23H-porphine cobalt (II)、
5,10,15,20-Tetrakis(pentafluorophenyl)-21H,23H-porphyrin iron (III)
chloride;
Step(2)Establish the standard finger-print of different brackets original plasm wine:
A:Different workshop different batches original plasm wines are chosen, include foreshot, level-one wine, second level per a batch of original plasm wine
Wine, four class of liquor tailing take 10 samples for the different grades of original plasm wine of each batch respectively, measure each sample using national standard method
The concentration of this ethyl alcohol and ethyl hexanoate;
B:Each sample takes 1 ~ 5ml original plasm wine to be put into specimen bottle, is placed in headspace gas generating device, pigment sensor
Array is placed at the top of specimen bottle, after sample head space contacts 2 ~ 10min with pigment sensor array, obtains different brackets original plasm wine
The image of corresponding pigment array obtains pigment array standard finger-print by signal extraction;
Step(3)The foundation of original plasm wine grade identification model:
A:Using ethyl alcohol known to original plasm wine and ethyl hexanoate concentration as output valveY, different brackets original plasm wine is corresponding
Pigment sensor array finger-print data-signal is as input variableX i, obtain the quantitative model of ethyl alcohol and ethyl hexanoate concentrationY It is quantitative=F(X i);
B:Become the corresponding pigment sensor array finger-print data-signal of known different brackets original plasm wine as input
AmountX i, original plasm wine grade is as output valveZ, obtain the qualitative forecasting model of original plasm wine gradeZ Grade identifies=G(X i);
Step(4)The identification of original plasm wine grade to be measured:
A:Quantitative detection is placed in headspace gas and fills as a result, 1 ~ 5mL original plasm wine sample to be measured is taken to be put into specimen bottle
It sets, pigment sensor array is placed in sample to be tested top of bottle, sample head space and 2 ~ 10min of pigment sensor array haptoreaction
Afterwards, original plasm wine sample map to be measured is obtained, the finger-print data-signal of original plasm wine sample to be measured is entered into step(3)The original of foundation
Starch the quantitative model of wine ethyl alcohol and ethyl hexanoate concentrationY It is quantitative=F(X i), it is dense to predict ethyl alcohol and ethyl hexanoate in original plasm wine to be measured
Degree;
B:Qualitative detection is as a result, according to ethyl alcohol in original plasm wine and ethyl hexanoate concentration, by sample to be tested spectrum data signal
Bring step into(3)In qualitative forecasting modelZ Grade identifies=G(X i) in, identify the grade of original plasm wine.
Claims (2)
1. a kind of method of pigment array sensing technology Fast Evaluation magma Quality of Liquors, it is characterised in that include the following steps:
(1)Integrated pigment sensor array:
Choose two or more air-sensitive pigment, according to Authorization Notice No. be 101074931 B of CN disclosed in color-sensitive gas pass
The production method of sensor array makes pigment sensor array;
(2)Establish the standard finger-print of different brackets original plasm wine:
A:Different workshop different batches original plasm wines are chosen, include foreshot, level-one wine, second level wine, wine per a batch of original plasm wine
Four class of tail takes multiple samples for the different grades of original plasm wine of each batch respectively, measures each sample second using national standard method
The concentration of pure and mild ethyl hexanoate;
B:Each sample takes 1 ~ 5ml original plasm wine to be put into specimen bottle, is placed in headspace gas generating device, pigment sensor array
It is placed at the top of specimen bottle, after sample head space contacts 2 ~ 10min with pigment sensor array, it is corresponding to obtain different brackets original plasm wine
Pigment array image, pigment array standard finger-print is obtained by signal extraction;
(3)The foundation of original plasm wine grade identification model:
A:Using ethyl alcohol known to original plasm wine and ethyl hexanoate concentration as output valveY, by the corresponding pigment of different brackets original plasm wine
Sensor array finger-print data-signal is as input variableX i, obtain the quantitative model of ethyl alcohol and ethyl hexanoate concentrationY It is quantitative=F(X i);
B:Using the corresponding pigment sensor array finger-print data-signal of known different brackets original plasm wine as input variableX i,
Original plasm wine grade is as output valveZ, obtain the qualitative forecasting model of original plasm wine gradeZ Grade identifies=G(X i);
(4)The identification of original plasm wine grade to be measured:
A:Quantitative detection is placed in headspace gas generating device as a result, 1 ~ 5mL original plasm wine sample to be measured is taken to be put into specimen bottle,
Pigment sensor array is placed in sample to be tested top of bottle, after sample head space and 2 ~ 10min of pigment sensor array haptoreaction,
Original plasm wine sample map to be measured is obtained, the finger-print data-signal of original plasm wine sample to be measured is entered into step(3)The magma of foundation
The quantitative model of wine ethyl alcohol and ethyl hexanoate concentrationY It is quantitative=F(X i), predict ethyl alcohol and ethyl hexanoate concentration in original plasm wine to be measured;
B:Qualitative detection brings sample to be tested spectrum data signal into as a result, according to ethyl alcohol in original plasm wine and ethyl hexanoate concentration
Step(3)In qualitative forecasting modelZ Grade identifies=G(X i) in, identify the grade of original plasm wine.
2. a kind of method of pigment array sensing technology Fast Evaluation magma Quality of Liquors according to claim 1, feature
It is:Air-sensitive pigment selected by pigment sensor array is Bromocresol Green, Bromothymol Blue, Thymol
Blue、Cresol Red、Bromocresol Purple、Neutral Red、
5,10,15,20-tetraphenyl-21H,23H-porphin、
2,3,7,8,12,13,17,18-Octaethyl-21H,23H-porphine manganese (III) chloride、
5,10,15,20-Tetraphenyl-21H,23H-porphine manganese (III) chloride、
5,10,15,20-Tetraphenyl-21H,23H-porphin; 5,10,15,20-Tetraphenyl-21H,23H-
porphine copper (II)、
5,10,15,20-Tetraphenyl-21H,23H-porphine iron (III) chloride;、
5,10,15,20-Tetrakis(4-methoxyphenyl)-21H,23H-porphine iron (III)
chloride;、
5,10,15,20-Tetraphenyl-21H,23H-porphine zinc、
5,10,15,20-Tetrakis(4-methoxyhenyl)-21H,23H-porphine cobalt (II)、
5,10,15,20-Tetrakis(pentafluorophenyl)-21H,23H-porphyrin iron (III)
In chloride any two or it is two or more.
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