CN106290592A - Highly effective liquid phase chromatographic device and method of work thereof - Google Patents
Highly effective liquid phase chromatographic device and method of work thereof Download PDFInfo
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- CN106290592A CN106290592A CN201510252372.8A CN201510252372A CN106290592A CN 106290592 A CN106290592 A CN 106290592A CN 201510252372 A CN201510252372 A CN 201510252372A CN 106290592 A CN106290592 A CN 106290592A
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- 239000007791 liquid phase Substances 0.000 title claims abstract description 55
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000004140 cleaning Methods 0.000 claims abstract description 76
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 74
- 238000005070 sampling Methods 0.000 claims abstract description 35
- 238000004458 analytical method Methods 0.000 claims abstract description 31
- 238000005259 measurement Methods 0.000 claims abstract description 27
- 238000012423 maintenance Methods 0.000 claims abstract description 21
- 239000012530 fluid Substances 0.000 claims abstract description 19
- 238000004128 high performance liquid chromatography Methods 0.000 claims abstract description 10
- 238000004440 column chromatography Methods 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 44
- 238000002347 injection Methods 0.000 claims description 29
- 239000007924 injection Substances 0.000 claims description 29
- 239000012071 phase Substances 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 5
- 238000012544 monitoring process Methods 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 description 22
- 239000012535 impurity Substances 0.000 description 20
- 239000002699 waste material Substances 0.000 description 19
- 238000010276 construction Methods 0.000 description 12
- 239000000306 component Substances 0.000 description 9
- 241000218158 Clematis Species 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000007445 Chromatographic isolation Methods 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 206010018910 Haemolysis Diseases 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 230000008588 hemolysis Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000002572 peristaltic effect Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241000601186 Clematis tangutica Species 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000008358 core component Substances 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 210000003677 hemocyte Anatomy 0.000 description 1
- 229940000351 hemocyte Drugs 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Abstract
The present invention provides a kind of highly effective liquid phase chromatographic device, and it has measurement pattern and service mode.This highly effective liquid phase chromatographic device includes sampling system, analyzes system and control system.This sampling system is for being supplied to this analysis system by sample to be tested mutually with flowing.This analysis system includes chromatographic column and detector.This control system is used for controlling this sampling system and this analysis system.This analysis system also includes filter, and is arranged at the diverter between this filter and this chromatographic column.This diverter makes filter be in different fluid path under this measurement pattern and this service mode.In measurement mode, this sample to be tested enters this chromatographic column chromatography via filter, diverter.In the maintenance mode, the first cleaning reagent is discharged via filter, diverter, cleans this filter when this first cleaning reagent is via this filter.Present invention also offers above-mentioned high performance liquid chromatography method of work.
Description
Technical field
The present invention relates to a kind of highly effective liquid phase chromatographic device and method of work thereof.
Background technology
Chromatographic column is the core component determining highly effective liquid phase chromatographic device analytical performance.Sample component analysis, such as glycolated hemoglobin analysis is carried out, it is common that separated by chromatographic column by the blood sample after haemolysis processes based on high performance liquid chromatography (HPLC) device.Haemolysis sample so contains the impurity such as substantial amounts of cell membrane fragments, and the grumeleuse in blood.Along with the increase of chromatographic column analysis times, these impurity can be trapped in chromatographic column, causes the flow resistance of chromatographic column to rise, and post pressure raises, and the serious post effect that also can make declines, and affects the accuracy of sample analysis, finally has to change chromatographic column.At present, defecator is generally used to protect before chromatographic column carries out post.
So defecator increasing along with testing time, also can impurity blocking in analyzed sample, pressure drop raises, and after the upper pressure limit that pressure drop is increased to system, needs to change filter or pre-column, not only makes troubles to user, also increase use cost.
Summary of the invention
In view of the foregoing, it is necessary to highly effective liquid phase chromatographic device and the method for work thereof of a kind of filter free of replacement are provided.
A kind of highly effective liquid phase chromatographic device, it has measurement pattern and service mode.This highly effective liquid phase chromatographic device includes sampling system, analyzes system and control system.This sampling system is for being supplied to this analysis system by sample to be tested mutually with flowing.This analysis system includes chromatographic column and detector, and this flowing forms continuous print liquid stream under the promotion of this sampling system, and this chromatographic column separates the component to be measured in sample to be tested under the effect of this liquid stream, and this detector detects the signal of this component to be measured.This control system is used for controlling this sampling system and this analysis system.This analysis system also includes filter, and is arranged at the diverter between this filter and this chromatographic column.This diverter makes filter be in different fluid path under this measurement pattern and this service mode.In measurement mode, this sample to be tested enters this chromatographic column chromatography via filter, diverter.In the maintenance mode, the first cleaning reagent is discharged via filter, diverter, cleans this filter when this first cleaning reagent is via this filter.
This highly effective liquid phase chromatographic device also includes pressure transducer, when this pressure transducer record when at least the flow path system pressure including filter exceedes preset value, sending information to this control system, this control system exports warning message and/or starts this service mode.
This sampling system includes syringe, injection valve, quantitatively encloses and for providing the constant flow pump of this flowing phase, under this measurement pattern, sample to be tested is injected this through this injection valve and quantitatively encloses by syringe, and this flowing drives the sample to be tested being collected in this quantitative circle to flow to this chromatographic column mutually；Under this service mode, the first cleaning reagent is injected this through this injection valve and quantitatively encloses by this syringe, and this flowing drives first cleaning reagent being collected in this quantitative circle to flow to this filter mutually.
This highly effective liquid phase chromatographic device also includes additional liquid circuit module, and this additional liquid circuit module has the power source providing the first cleaning reagent to enter this filter.
This additional liquid circuit module is directly connected with this filter, and this first cleaning reagent first cleans this filter and discharges through this diverter.
This additional liquid circuit module is directly connected with this diverter, and this first cleaning reagent enters this filter via this diverter, and cleans this filter, then discharges through this diverter.
A kind of high performance liquid chromatography method of work, it comprises the steps:
Control system sends and starts maintenance instruction, changes diverter, makes filter and chromatographic column disconnect；
First cleaning reagent is delivered to this filter, and cleans this filter；
Flowing is delivered to mutually this filter, and cleans the abluent of this filter residual；
This control system receives maintenance and completes instruction and determine beginning measurement instruction, changes diverter, makes this filter be connected with this chromatographic column；
Sample to be tested is delivered to this filter, and enters chromatographic column chromatography via diverter.
This high performance liquid chromatography method of work also includes pressure before the post of this chromatographic column of pressure sensor monitoring, when before this post, pressure exceedes preset value, and output warning message and/or start service mode.
This first cleaning reagent and this sample to be tested common sample introduction pipeline.
This first cleaning reagent enters this filter by additional liquid circuit module.
Comparing prior art, the highly effective liquid phase chromatographic device of the present invention is by being provided with diverter between filter and chromatographic column.When this highly effective liquid phase chromatographic device in the maintenance mode, the first cleaning reagent is via this filter and cleans this filter, with will be blocked in this filter impurity remove.The first cleaning reagent that this diverter controls from this filter is discharged is discharged through this diverter, thus solve filter and cause pressure drop rise to affect the problem of chromatographic isolation effect of chromatographic column because impurity blocking, also avoid user's frequent device for opening shell in use to carry out the replacing of filter, thus reduce use cost.The step that the high performance liquid chromatography method of work of the present invention comprises cleaned filter makes the chromatographic isolation of chromatographic column better.
Accompanying drawing explanation
Fig. 1 is the system construction drawing of the sample to be tested loading of the first better embodiment of highly effective liquid phase chromatographic device of the present invention.
Fig. 2 is the system construction drawing of the sample to be tested sample introduction of the first better embodiment of highly effective liquid phase chromatographic device of the present invention.
Fig. 3 is the system construction drawing of the cleaning reagent loading of the first better embodiment of highly effective liquid phase chromatographic device of the present invention.
Fig. 4 is the system construction drawing of the cleaning reagent sample introduction of the first better embodiment of highly effective liquid phase chromatographic device of the present invention.
Fig. 5 is the system construction drawing of the measurement pattern of the second better embodiment of highly effective liquid phase chromatographic device of the present invention.
Fig. 6 is the system construction drawing of the service mode of the second better embodiment of highly effective liquid phase chromatographic device of the present invention.
Fig. 7 is the system construction drawing of the measurement pattern of the 3rd better embodiment of highly effective liquid phase chromatographic device of the present invention.
Fig. 8 is the system construction drawing of the service mode of the 3rd better embodiment of highly effective liquid phase chromatographic device of the present invention.
Fig. 9 is the system construction drawing of the measurement pattern of the 4th better embodiment of highly effective liquid phase chromatographic device of the present invention.
Figure 10 is the system construction drawing of the service mode of the 4th better embodiment of highly effective liquid phase chromatographic device of the present invention.
Main element symbol description
| Highly effective liquid phase chromatographic device | 100,200,300,400 |
| Sampling system | 10 |
| Syringe | 11 |
| Injection valve | 12 |
| Joint | 12a, 12b, 12c, 12d, 12e, 12f, 23a, 23b, 23c, 23d, 23e, 23f, 33a, 33b, 33c, 33d, 43a, 43b, 43c, 43d, 43e, 43f , 53a, 53b, 53c, 53d, 53e, 53f |
| Quantitatively circle | 13 |
| Specimen bottle | 14 |
| Sample introduction pipeline | 15 |
| Reagent bottle | 16 |
| Buffer bottle | 17 |
| Constant flow pump | 18 |
| Analysis system | 20 |
| Pressure transducer | 21 |
| Filter | 22 |
| Diverter | 23,33,43,53 |
| Chromatographic column | 24 |
| Detector | 25 |
| Recording equipment | 26 |
| Back pressure regulator device | 27 |
| Waste liquid outlet | 28 |
| Waste fluid channel | 29 |
| Control system | 30 |
| Additional liquid circuit module | 44,54 |
Following detailed description of the invention will further illustrate the present invention in conjunction with above-mentioned accompanying drawing.
Detailed description of the invention
As shown in Figure 1, Figure 2, shown in Fig. 3 and Fig. 4, the first better embodiment of highly effective liquid phase chromatographic device 100 of the present invention, including sampling system 10, analysis system 20 and control system 30, this control system 30 is used for controlling this sampling system 10 and this analysis system 20.This highly effective liquid phase chromatographic device 100 has measurement pattern and service mode.
In measurement mode, this sampling system 10 includes syringe 11, injection valve 12, quantitatively encloses 13, specimen bottle 14, sample introduction pipeline 15, buffer bottle 17 and constant flow pump 18.This sample introduction pipeline 15 at this syringe 11, this injection valve 12, this quantitatively encloses 13, this reagent bottle 14, connects between this buffer bottle 17 and this constant flow pump 18.
It will be appreciated by those skilled in the art that this sampling system 10 can also directly for the device of micro liquid transfer liquid to be loaded into analysis system 20, namely this sampling system 10 may not include this injection valve 12 and this quantitative circle 13.
It should be understood that in the maintenance mode, this specimen bottle 14 is stored for the reagent bottle 16 of the first cleaning reagent and is replaced, namely this first cleaning reagent and this sample to be tested common sample introduction pipeline 15.
This syringe 11 for being sucked into this quantitatively circle 13 by the first cleaning reagent of the sample to be tested being stored in this specimen bottle 14 or reagent bottle 16.It will be appreciated by those skilled in the art that and be not limited to syringe here, other devices that can realize micro liquid transfer can be used for the present invention.
This injection valve 12 e.g. n cross valve (n > 2) or the combination of multiple n cross valve.Further, this injection valve 12 e.g. three-way change-over valve, four-port conversion value, clematis stem switching valve or a combination thereof.This diverter 23 forms different fluid path under this measurement pattern and this service mode.
In the present embodiment, this injection valve 12 is clematis stem switching valve, if this injection valve 12 is provided with dry joint 12a, 12b, 12c, 12d, 12e, 12f, by driving motor (not shown) to switch over connection between the conversion mouth that each joint is adjacent.
Sample flow quantitatively can be manipulated it should be understood that this quantitatively encloses 13.
This constant flow pump 18 is for providing the flowing phase with constant flow rate.This buffer bottle 17 is used for storing flowing phase.This sample to be tested can be loaded into this analysis system 20 by flowing that this constant flow pump 18 provides mutually.
This analysis system 20 includes pressure transducer 21, filter 22, diverter 23, chromatographic column 24, detector 25, recording equipment 26, back pressure regulator device 27, waste liquid outlet 28.
In the present embodiment, the joint 23b of this diverter 23 connects waste fluid channel 29.
It will be appreciated by those skilled in the art that the joint 23c of this diverter 23 also can connect other liquid circuit module (not shown).
This pressure transducer 21 is for monitoring the flow path system pressure including this filter 22.This pressure transducer 21 is arranged on the stream before this chromatographic column 24, can be between this filter 22 and this diverter 23, or between this diverter 23 and this chromatographic column 24, or this is quantitatively before circle 13 and this filter 22, or between this constant flow pump 18 and this injection valve 12.When the post pressure before this chromatographic column 24 exceedes preset value, sending information to this control system 30, this control system 30 alert, to point out user maintenance work to be carried out.
This filter 22 e.g. screen cloth or filter element.This chromatographic column 24 is for becoming some one-components by tested Component seperation.This detector 25 is for measuring the response signal that each one-component produces in this detector 25.This recording equipment 26 is for recording the electrical signal data that this detector 25 records.This back pressure regulator device 27 can be back pressure regulating valve.This waste liquid outlet 28 discharges waste liquid.This waste fluid channel 29 is for discharging the first cleaning reagent via this filter 22 and impurity.
This diverter 23 e.g. n cross valve (n > 2) or the combination of multiple n cross valve.Further, this diverter 23 e.g. three-way change-over valve, four-port conversion value, clematis stem switching valve or a combination thereof.This diverter 23 forms different fluid path under this measurement pattern and this service mode.
In the present embodiment, this diverter 23 is clematis stem switching valve, if this diverter 23 is provided with dry joint 23a, 23b, 23c, 23d, 23e, 23f, by driving motor (not shown) to switch over connection between the conversion mouth that each joint is adjacent.
Seeing also Fig. 1 and Fig. 2, when this highly effective liquid phase chromatographic device 100 carries out sample to be tested measurement, sample to be tested is loaded by first this sampling system 10.By pipeline, this syringe 11 is connected with this joint 12b；The joint 12a of this injection valve 12 is connected with joint 12b, and joint 12c is connected with joint 12d, and joint 12e is connected with joint 12f；These two ends quantitatively enclosing 13 are connected with joint 12a and joint 12d respectively；This specimen bottle 14 is connected with this joint 12 c.The sample to be tested of storage in this specimen bottle 14 is drawn to quantitatively enclose 13 by this syringe 11, thus completes the loading of this sample to be tested.
Secondly, by the conversion of this injection valve 12, jointing 12a and joint 12f, joint 12e are connected with joint 12d, and joint 12b is connected with joint 12c, are directly connected with this filter 22 so that this quantitatively encloses 13.
Further, by the conversion of this diverter 23, jointing 23a and joint 23f, joint 23e are connected with joint 23d, and joint 23b is connected with joint 23c, so that this filter 22 is directly connected with this chromatographic column 24.
It is appreciated that the conversion of this diverter 23 can complete conversion before this tested sample loads, it is also possible to during this tested sample loads, complete conversion with this injection valve 12 simultaneously.
It should be understood that in the present embodiment, this injection valve 12 and this diverter 23 are by driving motor (not shown) to carry out position switching.
Subsequently, the flowing of the constant flow rate that this constant flow pump 18 is provided enters this quantitatively circle 13 through joint 12a and joint 12f, and send after this sample to be tested eluting that quantitatively circle 13 is collected, enter this filter 22 through joint 12d and joint 12e to filter, then pass through joint 23a and joint 23f and enter this chromatographic column 24.This sample to be tested enters detector 25 through joint 23e and joint 23d after separating in this chromatographic column 24, and is detected each composition successively by this detector 25.
It should be understood that tested component is separated into single component after this chromatographic column 24, and flow to this detector 25.In this detector 25, the concentration of sample components is converted into the signal of telecommunication and sends this recording equipment 26 to, prints with collection of illustrative plates form or carries out follow-up data process.Waste liquid during measurement is discharged to this waste liquid outlet 28.
Seeing also Fig. 3 and Fig. 4, when this highly effective liquid phase chromatographic device 100 carries out maintenance work, the first cleaning reagent is loaded by first this sampling system 10.This syringe 11 is connected with this joint 12b；The joint 12a of this injection valve 12 is connected with joint 12b, and joint 12c is connected with joint 12d, and joint 12e is connected with joint 12f；These two ends quantitatively enclosing 13 are connected with joint 12a and joint 12d respectively；This reagent bottle 16 is connected with this joint 12 c.First cleaning reagent of storage in this reagent bottle 16 is drawn to quantitatively enclose 13 by this syringe 11, thus completes the loading of this first cleaning reagent.
Secondly, by the conversion of this injection valve 12, jointing 12a and joint 12f, joint 12e are connected with joint 12d, and joint 12b is connected with joint 12c, are directly connected with this filter 23 so that this quantitatively encloses 13.
Further, by the conversion of this diverter 23, jointing 23a and joint 23b, joint 23c are connected with joint 23d, and joint 23e is connected with joint 23f, so that this filter 23 disconnects with this chromatographic column 24.
It will be appreciated by those skilled in the art that the joint 23c of this diverter 23 also can connect other liquid circuit module (not shown).
It is appreciated that the conversion of this diverter 23 can complete conversion before this cleaning reagent loads, it is also possible to during this cleaning reagent loads, complete conversion with this injection valve 12 simultaneously.
It should be understood that in the present embodiment, this injection valve 12 and this diverter 23 are by driving motor (not shown) to carry out position switching.
Subsequently, the flowing that this constant flow pump 18 is provided enters this quantitatively circle 13 through joint 12a and joint 12f, and send after this first cleaning reagent eluting that quantitatively circle 13 is collected, this filter 23 is entered through joint 12d and joint 12e, and cleaning this filter 23, the first cleaning reagent and other impurity discharged from this filter are discharged to this waste fluid channel 29 through joint 23a and joint 23b.
It should be understood that this first cleaning reagent can be repeated several times be loaded, sampling action, in order to the impurity being blocked in this filter is cleaned up.After loading abluent cleaning, the first abluent of residual in filter 23 can be removed with the second cleaning reagent.The flowing phase stored in this second cleaning reagent e.g. this buffer bottle 17 or other buffer reagents.
It should be understood that this first cleaning reagent will be by being blocked in the impurity of this filter 23, e.g. hemocyte film fragment, blood clot etc., use the principles of chemistry that impurity resolves into less material, to be passed to the aperture of this filter 23, thus effectively eliminate the pressure drop of this filter 23.
It should be understood that increase diverter before this chromatographic column 24, so that this first cleaning reagent is not passed through this chromatographic column 24 when this filter 23 of clean and maintenance, and flow to this waste fluid channel 33, the abluent impact on chromatographic column can be avoided.
In the present embodiment, in measurement mode, this sampling system 10 loads the sample to be tested of predetermined to this highly effective liquid phase chromatographic device 100, pushes sample to be tested mutually with flowing, enters this chromatographic column 24 chromatograph via this filter 22, this diverter 23.During the measurement of this sample to be tested, this pressure transducer 21 can monitor pressure before the post of this chromatographic column 24 in real time, when pressure exceedes preset value before the post of this chromatographic column 24 recorded, sending information to this control system 30, this control system 30 controls to start service mode work.In the maintenance mode, this first cleaning reagent is injected flowing phase by this sampling system 10, first cleaning reagent being loaded into this filter 22 subsequently, and cleans this filter 22, the first cleaning reagent and the impurity discharged via this filter 22 are discharged to waste fluid channel 29 through this diverter 23.After cleaning, with the abluent of the second cleaning reagent cleaned filter residual to complete to safeguard.
It should be understood that in other embodiments, this highly effective liquid phase chromatographic device can also manually boot service mode work as required.Such as, when pressure transducer 21 detect pressure exceed preset value time, send information to this control system 30, this control system 30 alert, by human-computer interaction interface, point out user's attended operation to be carried out.After user is by human-computer interaction interface input maintenance instruction, this control system 30 obtains maintenance instruction, starts service mode work.
Highly effective liquid phase chromatographic device of the present invention, by being provided with pressure transducer, at least flow path system pressure including filter with real-time monitoring.When this pressure recorded exceedes preset value, this highly effective liquid phase chromatographic device can start service mode work automatically.In the maintenance mode, the impurity of plugged filter is cleaned by the first cleaning reagent repeatedly until below this pressure drop as little as preset value, thus realizing user's filter free of replacement or pre-column.
See also Fig. 5 and Fig. 6, the system construction drawing of the second better embodiment of highly effective liquid phase chromatographic device 200 of the present invention.
This sampling system 10, this analysis system 20 and this control system 30 are basically identical with the structure of the first better embodiment.Except for the difference that, this diverter 33 is four-port conversion value.If this diverter 33 is provided with dry joint 33a, 33b, 33c, 33d.This first cleaning reagent is loaded into this filter 22 mutually through the flowing that this constant flow pump 18 is provided, and cleans this filter 22, and the first cleaning reagent that this diverter 33 controls from this filter 22 is discharged flows to this waste liquid outlet 28.
In the present embodiment, this first cleaning reagent is identical with the sample introduction pipeline 15 of sample to be tested.
Referring to Fig. 5, when this highly effective liquid phase chromatographic device 200 carries out sample to be tested measurement, similar to Fig. 1 to Fig. 2, sample to be tested is loaded by first this sampling system 10.
Further, by the conversion of this diverter 33, jointing 33a and joint 33b, joint 33c are connected with joint 33d, so that this filter 22 is directly connected with this chromatographic column 24.
It should be understood that in the present embodiment, this injection valve 12 and this diverter 23 are by driving motor (not shown) to carry out position switching.
Subsequently, the flowing of the constant flow rate provided by the constant flow pump 18 of analysis system 20 enters this quantitatively circle 13 through joint 12a and joint 12f, and send after this sample to be tested eluting that quantitatively circle 13 is collected, enter this filter 22 through joint 12d and joint 12e to filter, then pass through joint 33b and joint 33a and enter this chromatographic column 24.This sample to be tested enters detector 25 through joint 33d and joint 33c after separating in this chromatographic column 24, and is detected each composition successively by this detector 25.
Referring to Fig. 6, when this highly effective liquid phase chromatographic device 200 carries out maintenance work, similar to Fig. 3 to Fig. 4, the first cleaning reagent is loaded by first this sampling system 10.
Further, by the conversion of this diverter 33, jointing 33a and joint 33d, joint 33b are connected with joint 33c, so that this filter 22 disconnects with this chromatographic column 24.
It should be understood that in the present embodiment, this injection valve 12 and this diverter 23 are by driving motor (not shown) to carry out position switching.
Subsequently, the flowing of the constant flow rate provided by the constant flow pump 18 of analysis system 20 enters this quantitatively circle 13 through joint 12a and joint 12f, and send after this first cleaning reagent eluting that quantitatively circle 13 is collected, this filter 22 is entered through joint 12d and joint 12e, and cleaning this filter 22, the first cleaning reagent and other impurity discharged from this filter 22 subsequently are discharged to this waste liquid outlet 28 through joint 33b and joint 33c.
Seeing also Fig. 7 and Fig. 8, the 3rd better embodiment of highly effective liquid phase chromatographic device 300 of the present invention, similar to Fig. 3 and Fig. 4, this highly effective liquid phase chromatographic device 300 includes sampling system 10, analysis system 20 and control system 30.
This sampling system 10, this analysis system 20 and this control system 30 are basically identical with the structure of the first better embodiment.Except for the difference that, additional liquid circuit module 44 is directly connected with this filter 22.This additional liquid circuit module 44 has the power source providing the first cleaning reagent to enter filter 22.Additional liquid circuit module 44 can this high performance liquid chromatograph other high pressure or low pressure liquid circuit module, power and pipeline are provided when filter needs to safeguard, the first cleanout fluid and the second cleanout fluid are transported to filter.Can be constant flow pump as similar in sampling system and pipeline, it is also possible to be general low-lift pump, peristaltic pump etc. and corresponding pipeline, transport as long as abluent can be realized.
It should be understood that this diverter 43 e.g. clematis stem switching valve.If this diverter 43 is provided with dry joint 43a, 43b, 43c, 43d, 43e, 43f, by driving motor (not shown) to switch over connection between the conversion mouth that each joint is adjacent.
Referring to Fig. 7, when this highly effective liquid phase chromatographic device 300 carries out sample to be tested measurement, similar to Fig. 1 and Fig. 2, sample to be tested is loaded by first this sampling system 10.
Further, by the conversion of this diverter 43, jointing 43a and joint 43f, joint 43e are connected with joint 43d, and joint 43b is connected with joint 43c, so that this filter 22 is directly connected with this chromatographic column 24.
It should be understood that in the present embodiment, this injection valve 12 and this diverter 23 are by driving motor (not shown) to carry out position switching.
Further, the flowing of the constant flow rate provided by the constant flow pump 18 of analysis system 20 enters this quantitatively circle 13 through joint 12a and joint 12f, and send after this sample to be tested eluting that quantitatively circle 13 is collected, enter this filter 22 through joint 12d and joint 12e to filter, then pass through joint 43a and joint 43f and enter this chromatographic column 24.This sample to be tested enters detector 25 through joint 43e and joint 43d after separating in this chromatographic column 24, and is detected each composition successively by this detector 25.
Referring to Fig. 8, when this highly effective liquid phase chromatographic device 300 carries out maintenance work, the first cleaning reagent is loaded by first this additional liquid circuit module 44.By the conversion of this diverter 43, jointing 43a and joint 43b, joint 43c are connected with joint 43d, and joint 43e is connected with joint 43f, so that this filter 22 disconnects with this chromatographic column 24.
It should be understood that in the present embodiment, this injection valve 12 and this diverter 23 are by driving motor (not shown) to carry out position switching.
Further, the first cleaning reagent provided by this additional liquid circuit module 44 is injected into this filter 22, and cleaning this filter 22, the first cleaning reagent and other impurity discharged from this filter 22 subsequently are discharged to this waste fluid channel 29 through joint 43a and joint 43b.
It should be understood that the first cleaning reagent and other impurity discharged from this filter 22 may also pass through joint 43a and joint 43b and discharges to this waste liquid outlet 28.
See also Fig. 9 and Figure 10, the system construction drawing of the 4th better embodiment of highly effective liquid phase chromatographic device 400 of the present invention.Similar to Fig. 3 and Fig. 4, this highly effective liquid phase chromatographic device 400 includes sampling system 10, analysis system 20 and control system 30.
It should be understood that this sampling system 10, this analysis system 20 are consistent with the structure of the first better embodiment with this control system 30.
Except for the difference that, this diverter 53 is joined directly together with additional liquid circuit module 54 and connects, and this diverter 53 controls this first cleaning reagent and firstly flows through this filter 22 and be then exhausted to this waste fluid channel 29.
It should be understood that this additional liquid circuit module 54 has the power source providing the first cleaning reagent to enter filter 22.
In the present embodiment, it is achieved the reverse cleaning to this filter 22, cleaning performance is more preferable.
Referring to Fig. 9, when this highly effective liquid phase chromatographic device 300 carries out sample to be tested measurement, sample to be tested is loaded by first this sampling system 10.
Further, by the conversion of this diverter 53, jointing 53a and joint 53b, joint 53c are connected with joint 53d, and joint 53e is connected with joint 53f, so that this filter 22 is directly connected with this chromatographic column 24.
It should be understood that in the present embodiment, this injection valve 12 and this diverter 23 are by driving motor (not shown) to carry out position switching.
Subsequently, the flowing of the constant flow rate provided by the constant flow pump 18 of analysis system 20 enters this quantitatively circle 13 through joint 12a and joint 12f, and send after this sample to be tested eluting that quantitatively circle 13 is collected, enter this filter 22 through joint 12d and joint 12e to filter, then pass through joint 53f and joint 53e and enter this chromatographic column 24.This sample to be tested enters detector 25 after separating in this chromatographic column 24, and is detected each composition successively by this detector 25.
Refer to Figure 10, when this highly effective liquid phase chromatographic device 400 carries out maintenance work, conversion by this diverter 53, jointing 53a and joint 53b, joint 53c is connected with joint 53d, joint 53e is connected with joint 53f, so that this filter 22 disconnects with this chromatographic column 24, makes this liquid circuit module 54 additional be connected with this filter 22.Additional liquid circuit module 54 can this high performance liquid chromatograph other high pressure or low pressure liquid circuit module, power and pipeline are provided when filter needs to safeguard, the first cleanout fluid and the second cleanout fluid are transported to filter.Can be constant flow pump as similar in sampling system and pipeline, it is also possible to be general low-lift pump, peristaltic pump etc. and corresponding pipeline, transport as long as abluent can be realized.
It should be understood that in the present embodiment, this injection valve 12 and this diverter 23 are by driving motor (not shown) to carry out position switching.
Further, first cleaning reagent is entered this filter 22 through joint 53c with joint 53 by additional liquid circuit module 54, and cleaning this filter 22, the first cleaning reagent and other impurity discharged from this filter 22 subsequently are discharged to this waste fluid channel 29 through joint 53a and joint 53b.Additional liquid circuit module 54 provides flowing to clean the abluent of residual in this filter 22 mutually by other pipelines again.
It should be understood that in above-mentioned four embodiments, by being provided with pressure transducer, when this pressure that this pressure transducer records exceedes preset value, this highly effective liquid phase chromatographic device can start service mode work automatically.Additionally, this highly effective liquid phase chromatographic device can also manually boot service mode work as required.Specific works process is as previously mentioned.
May be appreciated, by the first cleaned filter of cleaning reagent, so that the impurity being blocked in this filter resolves into little material and by the aperture of this filter, the first cleaning reagent and impurity after cleaning flow to waste fluid channel simultaneously, thus preventing the first cleaning reagent from entering chromatographic column, it is to avoid chromatographic column is undermined.
Highly effective liquid phase chromatographic device of the present invention, by being provided with pressure transducer, at least flow path system pressure including filter with real-time monitoring.When this pressure recorded exceedes preset value, this highly effective liquid phase chromatographic device can start service mode work automatically.In the maintenance mode, the impurity of plugged filter is cleaned until below this pressure drop as little as preset value repeatedly by the first cleaning reagent.Clean and maintenance to filter, on the one hand solve filter causes pressure drop rise to affect the problem of chromatographic isolation effect of chromatographic column because impurity blocking, on the other hand avoid user's frequent device for opening shell in use to carry out the replacing of filter, thus reduce use cost.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention being not restricted to the described embodiments, embodiment of above is only for explaining claims.So protection scope of the present invention is not limited to description.Any those familiar with the art is in the technical scope of present disclosure, and the change that can readily occur in or replacement, within being included in protection scope of the present invention.
Claims (10)
1. a highly effective liquid phase chromatographic device, it has measurement pattern and service mode, and it includes sampling system, analyzes system and control system, and this sampling system is for by sample to be tested with flow and be supplied to this analysis system mutually；This analysis system includes chromatographic column and detector, and this flowing forms continuous print liquid stream under the promotion of this sampling system, and this chromatographic column separates the component to be measured in sample to be tested under the effect of this liquid stream, and this detector detects the signal of this component to be measured；This control system is used for controlling this sampling system and this analysis system；This analysis system also includes filter, and it is arranged at the diverter between this filter and this chromatographic column, this diverter makes filter be in different fluid path under this measurement pattern and this service mode, and in measurement mode, this sample to be tested enters this chromatographic column chromatography via filter, diverter；In the maintenance mode, the first cleaning reagent is discharged via filter, diverter, cleans this filter when this first cleaning reagent is via this filter.
2. highly effective liquid phase chromatographic device as claimed in claim 1, it is characterized in that: this highly effective liquid phase chromatographic device also includes pressure transducer, when the pressure that this pressure transducer records exceedes preset value, sending information to this control system, this control system exports warning message and/or starts this service mode.
3. highly effective liquid phase chromatographic device as claimed in claim 1 or 2, it is characterized in that: this sampling system includes syringe, injection valve, quantitatively encloses and for providing the constant flow pump of this flowing phase, under this measurement pattern, sample to be tested is injected this through this injection valve and quantitatively encloses by syringe, and this flowing drives the sample to be tested being collected in this quantitative circle to flow to this chromatographic column mutually；Under this service mode, the first cleaning reagent is injected this through this injection valve and quantitatively encloses by this syringe, and this flowing drives first cleaning reagent being collected in this quantitative circle to flow to this filter mutually.
4. highly effective liquid phase chromatographic device as claimed in claim 1 or 2, it is characterised in that: this highly effective liquid phase chromatographic device also includes additional liquid circuit module, and this additional liquid circuit module has the power source providing the first cleaning reagent to enter this filter.
5. highly effective liquid phase chromatographic device as claimed in claim 4, it is characterised in that: this additional liquid circuit module is directly connected with this filter, and this first cleaning reagent first cleans this filter and discharges through this diverter.
6. highly effective liquid phase chromatographic device as claimed in claim 4, it is characterised in that: this additional liquid circuit module is directly connected with this diverter, and this first cleaning reagent enters this filter via this diverter, and cleans this filter, then discharges through this diverter.
7. a high performance liquid chromatography method of work, it comprises the steps:
Control system sends and starts maintenance instruction, changes diverter, makes filter and chromatographic column disconnect；
First cleaning reagent is delivered to this filter, and cleans this filter；
Second cleaning reagent is delivered to this filter, and cleans the abluent of this filter residual；
This control system receives maintenance and completes instruction and determine beginning measurement instruction, changes diverter, makes this filter be connected with this chromatographic column；
Sample to be tested is delivered to this filter, and enters chromatographic column chromatography via diverter.
8. high performance liquid chromatography method of work as claimed in claim 7, it is characterized in that: this high performance liquid chromatography method of work is also include monitoring the flow path system pressure at least included including filter, when this pressure exceedes preset value, output warning message and/or startup service mode.
9. high performance liquid chromatography method of work as claimed in claim 7, it is characterised in that: this first cleaning reagent and this sample to be tested common sample introduction pipeline.
10. high performance liquid chromatography method of work as claimed in claim 7, it is characterised in that: this first cleaning reagent enters this filter by additional liquid circuit module.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110741251A (en) * | 2017-07-05 | 2020-01-31 | 深圳迈瑞生物医疗电子股份有限公司 | Liquid phase analyzer and analysis method used for the same |
| CN113049796A (en) * | 2021-03-17 | 2021-06-29 | 王奔 | POCT glycated hemoglobin analyzer |
| WO2022170676A1 (en) * | 2021-02-09 | 2022-08-18 | Genequantum Healthcare (Suzhou) Co., Ltd. | Conjugation device and method for producing conjugates |
| US11578297B2 (en) | 2021-02-09 | 2023-02-14 | Genequantum Healthcare (Suzhou) Co., Ltd. | Conjugation device and method for producing conjugates |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012145513A (en) * | 2011-01-14 | 2012-08-02 | Hitachi High-Technologies Corp | Analyzer |
| CN204203173U (en) * | 2014-11-27 | 2015-03-11 | 王峰 | A kind of liquid chromatograph |
| CN204203174U (en) * | 2014-11-27 | 2015-03-11 | 王峰 | A kind of liquid chromatograph |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110741251A (en) * | 2017-07-05 | 2020-01-31 | 深圳迈瑞生物医疗电子股份有限公司 | Liquid phase analyzer and analysis method used for the same |
| CN110741251B (en) * | 2017-07-05 | 2022-09-09 | 深圳迈瑞生物医疗电子股份有限公司 | Liquid phase analyzer and analysis method used for the same |
| WO2022170676A1 (en) * | 2021-02-09 | 2022-08-18 | Genequantum Healthcare (Suzhou) Co., Ltd. | Conjugation device and method for producing conjugates |
| US11578297B2 (en) | 2021-02-09 | 2023-02-14 | Genequantum Healthcare (Suzhou) Co., Ltd. | Conjugation device and method for producing conjugates |
| CN113049796A (en) * | 2021-03-17 | 2021-06-29 | 王奔 | POCT glycated hemoglobin analyzer |
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