CN106290367A - A kind of complicated fraction holographic imaging method of chemically based combination disturbance - Google Patents

A kind of complicated fraction holographic imaging method of chemically based combination disturbance Download PDF

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Publication number
CN106290367A
CN106290367A CN201610700220.4A CN201610700220A CN106290367A CN 106290367 A CN106290367 A CN 106290367A CN 201610700220 A CN201610700220 A CN 201610700220A CN 106290367 A CN106290367 A CN 106290367A
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disturbance
complicated
imaging
chemical
fraction
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CN201610700220.4A
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Chinese (zh)
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李剑超
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李剑超
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84Systems specially adapted for particular applications
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence

Abstract

The present invention establishes the i mage analysis methods of a kind of complicated fraction being common in environmental science, bioscience, medical science, Food Science.This technical method, based on high flux disturbance expression strategy, can be built a large-scale perturbation analysis system by limited species, provides the internal chemical information that complicated fraction to be measured is abundant.And made core devices preparation cost cheap by the disturbance egative film preparation that jet printing is core;Intensive detection execution greatly simplifies execution;And the artificial intelligence analysis for complex sample provides a big data acquisition approach.

Description

A kind of complicated fraction holographic imaging method of chemically based combination disturbance
Technical field
The present invention relates to the detection technique field of analytical chemistry.In particular it relates to environmental science, biological section The rendering method of complicated fraction Complex chemical information common in the practice such as, medical science, Food Science.
Background technology
Naturalness, many materials of artificial preparation or its ingredient exist with complicated fraction form;Citing For, such as sewage, river, the soil liquid, blood, urine, saliva, plant tissue liquid, beverage, TCM liquid extract etc..Solid The sample of form, uses some pre-treating methods to obtain the form being suitable for, and squeezes the juice such as by machinery or the method such as extraction, it is thus achieved that Complicated fraction can also be analyzed.And this complicated fraction occurs the most as a mixture.The complexity of this mixing Contain the interaction between various component inside solution, form the system of a relative equilibrium.In these complicated fraction The relation in portion is the most extremely complex, and quantity is the hugest.The analysis of one Sewage Environment sample is shown by we, wherein Inorganic, the main species of organic substance and form i.e. exceed hundreds of;By permutation and combination, the mutual relation between them surpasses Cross 9 powers of 10.Scientific and technical personnel create certain methods, such as composition measuring, simple Calculation of chemical equilibrium etc., measure and characterize These features.For the detection of solution composition, main employing chemical analysis and Instrumental Analysis.Wherein, divide with mass spectral analysis, chromatograph The conventional meanses such as analysis method, x-ray spectrometry, electrochemical methods and nuclear magnetic resonance spectroscopy are main investigative technique.But for Real complicated fraction but hardly results in a large amount of and comprehensive information, especially for each chemistry within complicated fraction The relation information linearly or nonlinearly interacted between species.
In recent years, utilize electrochemical signals to be detected as main electronic tongues or electric nasus system gradually grows up, in complexity Certain effect is served in the analysis of liquid, edema caused by disorder of QI.But due to its basis syntheti c route of sensing unit and measuring condition and code Requirement, it can not develop the sensor array of extensive quantity at low cost.And use owing to it needs realization-cleaning- The repeated detection re-used, so volatilizable or diffluent detector unit sensitive material can not be used.Separately there are some researchs logical Cross visual response (the color change that causes such as chemical action, change in fluorescence etc.) and complete sensor array detection, have With low cost, and the simple advantage of equipment needed thereby (list of references: one, Qian, S.and H.Lin (2015). " Colorimetric Sensor Array for Detection and Identification of Organophosphorus and Carbamate Pesticides. " Analytical Chemistry87 (10): 5395-5400. bis-, Schiller, A., B.Vilozny, R.A.Wessling and B.Singaram (2008). " Recognition of phospho sugars and nucleotides with an array of boronic acid appended bipyridinium salts.″ Analytica Chimica Acta 627 (2): 203-211. tri-, Schiller, A., R.A.Wessling and B.Singaram(2007).″A fluorescent sensor array for saccharides based on boronic acid appended bipyridinium salts.″Angewandte Chemie International Edition 46 (34): 6457-6459. tetra-, yellow star are grand, South Korea triumphant (2014). the making side of a kind of all solid state visualization taste sensor array Method. application publication number: CN104062292A. five, Wu Yu, Huo Danqun, Hou Changjun, etc. visualization sensor array is to oolong tea etc. The quick Study of recognition of level. assay office, 2015,34 (1): 1-5.).Their manufacture thinking is: n to be formed each opposite sex Cellular array, the most first prepares n kind sensitive material, then they is arranged in array.See list in the array of document case Unit's quantity is the most several, ten several, twenty or thirties, utilizes this strategy process to be difficult to obtain easily big tens, hundreds of The array of element number;Let alone thousand of, the array of several ten thousand grades.
For solving object, above mentioned prior art, it is mainly used in and specific components in testing sample is divided Analysis or sample classification.For the internal chemical relation of complicated fraction sample presents, due to substance classes therein comprehensively Various, mutual chemical relationship enormous amount.This big quantity is reflected merely with several, tens data measuring point The system of parameter, it is clear that be unpractical.
In recent years, along with the development of artificial intelligence technology, for realizing the internal relations to complicated sample itself, Yi Jifu The degree of depth of the interactively between miscellaneous sample and something outside is extensively analyzed and is provided possibility.But a precondition is intended to energy It is simply the internal chemical information that artificial intelligence provides the sample to be analyzed of big quantity.So scientific and technological circle expect and find a class The analysis that more can carry original determinand internal chemical relation information presents technology.The present invention i.e. pays close attention to this.
In order to be more fully understood that the method that the present invention proposes, referring initially to the analysis of a simple mechanism aspect.
Look first at this class phenomenon.A certain being in metastable complicated fraction L1, they are internal numerous various Chemical relationship all can be in the state of relative equilibrium.Now, we are by a kind of common detection method, such as the hands such as fluorescence, colour developing Section is analyzed operation, an available fluorescence intensity or the testing result of color to it, is labeled as F0.
With reference to the single operation 1 of Fig. 1, for original complicated fraction L1 to be analyzed, when there being a certain amount of composition solid After other fixed chemical substances R1 are added into, the meeting disturbance original numerous equilibrium relations of complexity fraction system more or less; And then allow the numerous chemical relationship in this system enter into another kind of poised state.Now use identical colour developing or fluoroscopic examination Method, we can obtain a testing result likely differing from F0, be labeled as F1.
If similarly we repeat previous step, but the chemical substance being separately added into is different from the R2 ... of R1, Rn; With reference to the batched operation 2 of Fig. 1, correspondingly we can be obtained by a series of testing result F2 ..., Fn.Disturbance compound Ri (i=1 ..., the increase of kind n) can dramatically increase the expressed in abundance degree to determinand.When this quantity increases to Thousand of, several ten thousand even more than million, quantity of information generation great change will be made.
It can be seen that Ri can be a kind of compound or mixture here.One forms the Ri of huge number cleverly Method is: can mix numerous kinds by the bigger chemical substance of a limited number of kind of nature difference according to different proportional amount The Ri of class.Such as can be allocated by the different proportional amount of 6 kinds of compounds;Simple computation, when with each compound 1% be changed to split unit time, number of combinations kind is about 6 powers of 100.It addition, these six kinds of basic allotments " raw material ", each It itself can also be mixture.
In order to provide parallel, the big order of magnitude, the chemical disturbance junior unit of regularization characteristics of combination, we are based on height Flux (HTE) principle, carries out the HTE combination (seeing the high flux combination 3 of Fig. 1) of system for numerous Rn.Mainly pass through On flat carrier, regularization independent dispersion big quantification degree point realizes.In the face of when assembling of so big quantity, utilize ink Water jet printing technology carries out operation can make whole operation become quick and low cost.It practice, ink jet print operation is A kind of high efficiency method being assigned on two dimensional surface carrier that fraction materials quantitative is positioned, and this complicated fraction can be Solution can also be the suspension of high degree of dispersion.Particularly there is the color inks jet printer of multiple cartridge, set rationally In the case of putting print conditions, can be by allotment, print position and the setting of printing ink ejection amount to marking ink, it is achieved on The combination of disturbance chemical substance R that face is mentioned and arrangement space.Described utilizes ink jet print technology, in planar substrates Several setting regions, quantitatively add the chemical substance setting composition, be that printer is according to the printing electronic image masterplate set Control what ink ejection amount and ink jetting position realized.It addition, screen printing technique can also be by coming real to the adjustment of preparation condition More existing relatively simple preparation manipulations;The advantage of silk screen printing is that price is extremely low, and speed is faster.
Summary of the invention
In conjunction with above discussion, the particularly analysis to high throughput chemical combination disturbance, the present invention proposes new complicated liquid The internal bulk properties holographic imaging method of shape thing.Holographic imaging said herein, actual result is the inside number to complicated fraction Measuring numerous chemical relationship to carry out jumbo digitized and present and note down, its record is one to organize greatly chemical effect.Data are held Amount is according to design, the most tens of, hundreds of, the most thousands of tens thousand of or more.The super large provided out for a complicated fraction Effect data amount more can accurately portray comprehensive chemical property of this sample.
A kind of complicated fraction holographic imaging method of chemically based combination disturbance, at least includes step in detail below:
(1) ink jet print technology is utilized to prepare chemical combination disturbance holographic imaging egative film, the concrete operations of this step For:
(1.1) selected chemistry disturbance agent number, definition chemistry disturbance agent number is R-D;And selected R-D is more than or equal to Two;
(1.2) preparation R-D kind can be used for the chemical ink of ink jet print;In the composition of the arbitrary chemistry ink joined Containing at least one chemistry disturbance agent, and at least chemical ink joined contains one and dissolves in complicated fraction to be measured Chemical disturbance agent;During analyzing, this composition dissolved can be dispersed in complexity to be measured with the form of molecule or ion In fraction, directly participate in the foundation of aqueous chemical balance;Described chemical disturbance agent refers to a kind of compound or mixture, will It is added into complicated fraction to be measured, and the internal chemical balance of complicated fraction to be measured can be made to produce change;
(1.3) it is designed for guiding printer to carry out positioning and quantitative and sprays the electronic image masterplate of chemistry disturbance agent;
Described electronic image masterplate is made up of color dot, is uniform inside described color dot;The area of color dot I To the size that the minimum resolution unit of the electronic image masterplate designed is corresponding;In all of color dot, include guiding at least two Plant the color dot that chemistry disturbance agent ink sprays;Described electronic image masterplate can control the ink-jet operation of R-D independent cartridge;This Requirement can be realized by arranging of the pattern (RGB, CMYK etc.) of electronic image masterplate, figure layer, passage etc.;Lift with CMYK pattern For example, when controlling three independent cartridges, tri-passages of C, M, Y in an available figure layer, can be with two figures when controlling four Any four in six passages of C1, M1, Y1, C2, M2, Y2 in Ceng, the rest may be inferred by analogy;The file of electronic image can be by conversion etc. Operation obtains multiple appearance form, and the personnel with graphical design knowledge can complete;Described independent cartridge refers to ink On jet printer, the ink of each color is noted the space deposited;Several independent cartridges can be distributed on a print cartridge, it is possible to To be distributed on several print cartridge;
(1.4) the chemical ink prepared by R-D kind (1.2nd) step, utilizes ink-jet printer, according to (1.3rd) step The guiding printing electronic image masterplate of design, rationed setting position on substrate;
The controllable independent ink of printing electronic image masterplate of the species number of the chemical ink selected and the design of (1.3rd) step Storehouse quantity is identical;
(1.5) selection sprays, impregnates, prints one of three kinds of operational means or combination, positions and be applied with on planar substrates Imaging agents;Here the imaging agents applied can be equally distributed, it is also possible to has Two dimensional Distribution variation characteristic;Institute The imaging agents stated refers to can to produce some chemical property of complicated fraction to be measured or component color or fluorescence response Single or mix reagent;Such as developer, fluorescent probe or its mixture;Here the Two dimensional Distribution variation characteristic that has also refers to several Different imaging agents is distributed in the zones of different of approximately the same plane substrate;
Described substrate is ink jet printing paper, printing film, art paper, chromatography slide etc.;Although the substrate selected here is Uniform and without imaging agents, if but use and comprised specified chemical reagent, particularly comprise the base of imaging agents Sheet, dispenses execution certain step above, the most also meets the technology of the present invention route.
Closing before and after (1.4th) step, (1.5th) step perform and fasten, following sequence can reach similar effect, as (1.5th) step is placed on after (1.4th) step and performs, or (1.5th) step is placed on before (1.4th) step and performs, or by each for this two step It is divided into several operation, replaces or merge execution;Hereinafter described here replacing or merge execution is illustrated;For same One substrate, ink jet print, spray, impregnating operation can be implemented by several times in batches, as several inks print by several times, a kind of Ink prints by several times, imaging agents sprays by several times, several imaging agents sprays by several times, become imaging agents to use jet printing method Operate with chemistry disturbance agent simultaneously, use jet printing method printing by several times etc. as reagent.
In terms of physical property, described imaging agents, chemistry disturbance agent can with solid content or absorption the form on substrate It is present under room temperature and non-close environment;
(1.6) solvent is removed in volatilization, the chemical combination disturbance holographic imaging egative film described in dried acquisition;
Described chemical combination disturbance holographic imaging egative film is constituted by resolving to picture point;Described resolves to the internal one-tenth of picture point As reagent is equally distributed, and each chemical disturbance agent resolving to comprise in picture point is only by the color dot of (1.3rd) step Control to spray;And for the consideration of combinatorial chemistry quantity of information, in the case of lower limit set is for comprising Poor information demand, On one described chemical combination disturbance holographic imaging egative film, the mutually different quantity of picture point that resolves to is more than or equal to 50;
For a chemical combination disturbance holographic imaging egative film, the quantity of the quantity and color dot that resolve to picture point is to have necessarily Relation;When imaging agents is uniformly distributed, both are equal;(there is certain two dimension when imaging agents non-uniform Distribution Changes in distribution), then the former is more than or equal to the latter;Here parsing also refers to: the numerous information datas in holographic imaging caned by The lower limit that level is decomposed;
(2) complicated fraction quantitative and even to be measured is sprayed on the chemical combination disturbance holographic imaging egative film of preparation;
(3) the chemical combination disturbance holographic imaging egative film after (2nd) step effect utilize optical imaging device or fluorescence become As device carries out shooting imaging.
In the hologram of design and preparation, can be by splicing, the method for embedding, the survey that directly develops the color of concrete component Surely it is combined in hologram sheet;But the other parts in hologram sheet, if meeting the disturbance combination thinking of the present invention, then this part is also Protected by this patent;
For concrete example, described imaging agents is: acid-base indicator, such as bromocresol green, C.I. 13020., Herba Cladoniae rangiferinae etc.;Oxygen Change reduction indicator, such as methylene blue, diphenylamines etc.;Metal indicator, such as C.I. 14645., acid chromium blue k, calmagite etc.;And Now exploitation and fast-developing novel agent, such as the compound of porphyrin, phthalocyanine and derivatives class thereof, specific dye, Schiff Alkali etc., the most novel dye reagent for Measurement for Biotechnique.Described imaging agents can also be can be with complicated fraction In material covalently or non-covalently combine the fluorometric reagent forming fluorescence, common such as 8-hydroxyquinoline, flavonol, Punica granatum L. alizarin R, morin etc.;Or itself there is the fluorescent dye of fluorescence, such as fluorescein sodium, tetrabromofluorescein etc., and a large amount of novel use Fluorescent probe etc. in Measurement for Biotechnique.Described imaging agents mixing, refers to the imaging agents mixing of several individual use Together, use as a kind of imaging agents.
Chemistry disturbance agent is worked essentially by the chemical equilibrium participated within complicated fraction to be measured;So From simple compound to complicated mixture, this effect much can be realized.But want selection differences big, dry in practice Disturb the more efficiently species of effect.Because the distinct fluctuations of the image finally given is main still by chemistry perturbation action Rich realize.It addition, when selecting chemistry disturbance agent, it is also noted that do not make chemistry rich to imaging agents of disturbance agent Rich change has the negative effects such as too many coverage;The dark compound of such as colour stable is not the disturbance of good color development system Agent.Can also there be some directions when chemistry disturbance agent selects, such as, select several simple classes of compounds, such as acid-alkali accommodation Agent, chelating agent, ion competition agent, screening agent etc..
Chemistry disturbance agent can also be minute solid particles, and they can disperse in a liquid, their perturbation action Methods is: disturbed composition and the chemical equilibrium of complicated fraction to be measured by effects such as absorption, catalysis.As gold colloidal, The ultra-fine grain dispersion liquids etc. such as Ludox, ferrum collosol, nano zircite, nano yttrium oxide, nano-zinc sulfide.
The complicated fraction holographic imaging method of described a kind of chemically based combination disturbance, it is characterised in that: described Utilize the step for that ink jet print technology prepares chemical combination disturbance holographic imaging egative film before, further comprise the steps of: and carry out Imaging agents and the screening of chemistry disturbance agent for complicated fraction to be measured;Detailed process is:
By single or mixing imaging agents be quantitatively added into complicated fraction to be measured, determine its present be different from former multiple The color of miscellaneous fraction or fluorescent characteristic;Chemistry disturbance agent is quantitatively added into the above-mentioned to be measured complicated liquid that with the addition of imaging agents Shape thing, determines that it may result in being further change in of color or fluorescent characteristic.
Considering from increasing disturbance specific diversity, the complicated fraction holography of described a kind of chemically based combination disturbance becomes Image space method, it is characterised in that: in (1.1st) described step, selected R-D is more than or equal to three.
Considering from increasing disturbance specific diversity, in the case of having the change of relatively horn of plenty, described one is chemically based The complicated fraction holographic imaging method of combination disturbance, it is characterised in that: in (1.1st) described step, selected R-D is more than Yu Si.
Considering from increasing disturbance specific diversity, in the case of having the change of more horn of plenty, described one is chemically based The complicated fraction holographic imaging method of combination disturbance, it is characterised in that: in (1.1st) described step, selected R-D is more than In five.
For verification and the purpose of reduction error, electronic image masterplate arranges repetition color dot, demarcation color dot etc. is set.
In the case of reaching equally to guide printing purpose, electronic image masterplate is one, or two and above subgraph The a set of electronic image formed by modes such as plain splice, figure layer combinations.Can also be come by the mode of operation that color separation prints Design electronic image masterplate.
For the consideration of combinatorial chemistry quantity of information, in the case of lower limit set is for comprising relatively Poor information demand, described The complicated fraction holographic imaging method of a kind of chemically based combination disturbance, it is characterised in that: a described chemical group Closing on disturbance holographic imaging egative film, the mutually different quantity of picture point that resolves to is more than or equal to 100.
In the case of lower limit set is for comprising medium quantity of information demand, answering of described a kind of chemically based combination disturbance Miscellaneous fraction holographic imaging method, it is characterised in that: on a described chemical combination disturbance holographic imaging egative film, the most not phase The same quantity resolving to picture point is more than or equal to 200.
Under same core thinking, the method preparing chemical combination disturbance holographic imaging egative film according to this, it is also possible to same The imaging area that several chemical property is widely different is prepared on individual bare substrate;So can expand chemical combination disturbance holography Quantity of information as egative film.Its external form is equivalent to several different chemical combination disturbance holographic imaging egative film and splices one In individual plane, as the egative film that for subsequent detection is unified.This mode will be of density analysis accurate, big future Important form.
The complicated fraction holographic imaging method of described a kind of chemically based combination disturbance, it is characterised in that: described (3rd) step after, farther include: to shooting obtain image be further processed, obtain relatively sharp clear image Form, such as gray-scale map, color separation figure, binary map, Fourier transform figure etc..
The complicated fraction holographic imaging method of described a kind of chemically based combination disturbance, it is characterised in that: described It is to use jet spraying or ullrasonic spraying that complicated fraction quantitative and even to be measured sprays, and uses fixing spray amount and spray strip Part realizes;Described spray condition refers to the spacing of fog-spray nozzle type, fog outlet and chemical combination disturbance holographic imaging egative film With angle, hurricane globe size and position.
The complicated fraction holographic imaging method of described a kind of chemically based combination disturbance, it is characterised in that: described Complicated fraction to be measured is one of following sample: after urine after stain disease, the soil liquid, original or processing, original or processing Blood, beverage.
The present invention compared with prior art, has the advantage that and beneficial effect:
(1) a large-scale disturbance can be built by several compounds (the most several chemistry disturbance agent, imaging agents) to divide Analysis system, provides the abundant internal chemical information of complicated fraction to be measured.These chemical informations may be used for complicated fraction Qualitative or explore the exercising result of itself and other actor.
(2) due to the basic point of departure of this technology be use chemical constituent go the chemical equilibrium relation in disturbance determinand, So the result of its detection more embodies the dynamic chemical relation of the inside complexity of determinand.
(3) this technical method at least can perform according to complexity the most suddenly: chemical combination disturbance holographic imaging The preparation of egative film can be carried out standardization by specialty manufacturer and manufacture in a large number;Later stage, simple liquid application to be measured added and photographic actions, Can be performed by general layman.So simple to operate, the testing cost manufactured in batch is the cheapest.
(4) high flux disturbance-expression strategy that this technical method is set up, can pass through standardization, divide for artificial intelligence Analysis complicated chemical sample provides a big data acquisition approach.
Accompanying drawing explanation
Fig. 1 is the Analysis on Mechanism figure of the present invention.1-single operation, 2-batched operation, 3-high pass quantization operation.
Fig. 2 is the electronic image masterplate of design in embodiment 1.
Fig. 3 is the schematic diagram of complicated fraction quantitative ultrasound spraying weighing device to be measured in embodiment 1.1-ullrasonic spraying Device, 2-plastics pallet, 3-analytical balance.
Fig. 4 is the detection imaging results figure of the complicated fraction of embodiment 1.
Fig. 5 is the electronic image masterplate of middle design in embodiment 3.
Fig. 6 is the testing result figure of embodiment 3.
Fig. 7 is the electronic image masterplate of embodiment 4 design.
Fig. 8 is the electronic image masterplate of embodiment 5 design.
Fig. 9 is the testing result figure of embodiment 8.
Figure 10 is the metered dose device schematic diagram in embodiment 9.
Figure 11 is the experimental result picture of embodiment 10 repeatability checking.
Detailed description of the invention
Being described principle and the feature of the present invention below in conjunction with specific embodiments and the drawings, example is served only for solving Release the present invention, be not intended to limit the scope of the present invention.
The preparation of embodiment 1 complicated fraction sample to be measured
Have chosen from several samples such as plant, animal to beverage, medicine, environmental samples.
Plant: take watermelon flesh (western agriculture eight), tomato pulp (red No. 1 of capital), Fructus Cucumidis sativi sarcocarp (rich No. 6 of garden), Radix Dauci Sativae fruit Meat (red No. one of spring) apple pulp (Luochuan, Shaanxi red fuji apple), oriental plane tree leaves, Folium Pini etc., squeeze the juice respectively, filters, Respectively take 30mL supernatant, bottle stand-by.
Animal: buy live chickens, live crucian one, extract some blood, be stored in test tube stand-by.
Urine: take from the original urine in Hospital of Shaanxi Normal University laboratory.
Tea solution: weigh 10g black tea, oolong tea, green tea respectively, is separately added into 90g distilled water, 60 DEG C of water-bath 20min. Obtain black solution, oolong tea solution, green tea solution naturally cool to room temperature.Filter, respectively take supernatant 30mL, bottle stand-by.
Red wine solution: take claret (Shandong Province of China Yantai Zhangyu claret) 30mL, bottle stand-by.
Middle drug solns: Radix Isatidis granules and LIUWEI DIHUANG WAN are ground to powder, weigh 2g, adds 50ml water vibration 6h, Stand 2h, take supernatant bottling stand-by.
Bed mud Interstitial Water: take bed mud in river course, river, southern suburbs, Xi'an City, Shanxi Province, centrifugal, take supernatant 50mL, stand-by.
Percolate: take household garbage-compressing station, school district, Shaanxi Normal University Chang'an percolate, in shady and cool lucifuge Place places one week, filters, takes supernatant, stand-by.
Sanitary sewage: take Shaanxi Normal University's students' dormitory sanitary sewage.Filter, take supernatant, stand-by.
Humic acid solution: select industry humic acid powder, the humic acid solution of preparation 1g/L, be placed in refrigerator, stand-by.
The preparation of embodiment 2 chemical combination disturbance holographic imaging egative film and application examples thereof
The to be measured complicated fraction obtained for embodiment 1, performs the present invention and proposes answering of chemically based combination disturbance The internal character formation method of miscellaneous fraction, comprises the following steps that.(number of steps in specific embodiment likely differs from description In " summary of the invention " part numbering)
(1) imaging agents and the screening of chemistry disturbance agent are carried out
In order to coordinate overall holographic imaging egative film scheme, convenient observation contrasts, and takes 0.5ml complicated fraction to be measured, dropping On 20mm diameter filter paper, normal temperature drying, obtain complicated fraction-filter paper (being designated as sheet 1) to be measured.
Select 1,2,3-indantrione monohydrate as imaging agents to be determined, prepared 2% ninhydrin solution.Accurate weighing indenes three during preparation Ketone 0.2g, adds anhydrous alcohol solution and is settled to 10mL (needing now with the current).Observe contrast for convenience, take this liquid of 0.5ml, drip Being added on 20mm diameter filter paper, normal temperature drying removes solvent, obtains imaging agents-filter paper.Take 0.5ml complexity to be measured aqueous Thing, adds the imaging agents of above-mentioned preparation-filter paper, volatile dry, obtains sheet 2.
Select DTPA, Glycine sodium, potassium dihydrogen phosphate, Na2CO3、CuCl2