CN106282422B - A method of the separation and Extraction glucose from starch saccharificating liquid - Google Patents
A method of the separation and Extraction glucose from starch saccharificating liquid Download PDFInfo
- Publication number
- CN106282422B CN106282422B CN201610775617.XA CN201610775617A CN106282422B CN 106282422 B CN106282422 B CN 106282422B CN 201610775617 A CN201610775617 A CN 201610775617A CN 106282422 B CN106282422 B CN 106282422B
- Authority
- CN
- China
- Prior art keywords
- liquid
- mixed
- filtrate
- powder
- glucose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K1/00—Glucose; Glucose-containing syrups
- C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
- C13K1/08—Purifying
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K1/00—Glucose; Glucose-containing syrups
- C13K1/10—Crystallisation
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
- C02F2103/343—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the pharmaceutical industry, e.g. containing antibiotics
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/14—NH3-N
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Emergency Medicine (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention belongs to glucose processing technology fields, disclose a kind of method of separation and Extraction glucose from starch saccharificating liquid comprising following steps: step 1) prepares flocculating decoloring agent, step 2 filtering, step 3) flocculation decoloration, step 4) nanofiltration, step 5) crystallization, step 6) mother liquid disposal.The present invention uses flocculation purification decolorization and multi-filtering step, yield and purity is high, and investment and land occupation are reduced, and enterprise's input cost reduces.
Description
Technical field
The invention belongs to glucose processing technology fields, and in particular to one kind separation and Extraction glucose from starch saccharificating liquid
Method.
Background technique
Glucose (Glucose) (chemical formula C6H12O6) it is also known as corn sugar, corn sugar, referred to as glucose.English
Another name: Dextrose, Cornsugar, Grapesugar, Bloodsugar, be distributed in nature it is most wide and mostly important one
Kind monosaccharide, it is a kind of polyhydroxy aldehyde.Pure glucose be clear crystal, pleasantly sweet but sweet taste not as good as sucrose (common people without
Method tastes sweet taste), it is soluble easily in water, it is slightly soluble in ethyl alcohol, does not dissolve in ether.Aqueous solution optically-active to the right, therefore belongs to " dextrose ".Grape
Sugar has critical role in field of biology, is energy source and the metabolism intermediate product of living cells, i.e., biological is main
Energy supply material.Plant can generate glucose by photosynthesis.In candy making industry and field of medicaments extensive application.
Cornstarch is the important source material for extracting glucose, and corn starch molecules are connected by thousands of a glucose units
It connects, the technique that glucose is extracted from starch saccharificating liquid is then that the final step work of glucose is extracted from corn starch milk
Skill, equally, this step process are also related to the quality of glucose finished product.In order to reduce the by-product of glucose, people are in glucose
In processing technology step, a various solutions, relatively common technique are proposed are as follows: the life of existing production glucose
Production. art is as follows: being made from starch, by liquefying under the action of enzyme re-liquefied after sizing mixing, then again through saccharification enzyme effect sugar
Change, eventually form the glucose syrup of DX95% or so, then enters sheet frame by the way of diatomite filtering or rotary drum carried out
Filter, to remove remaining starch and suspended matter, then use fixed bed ion-exchange process, mainly using a large amount of yin-yang from
Sub-exchange resin removes the salinity in liquid glucose, finally obtains pharmaceutical grade glucose with the technique of recrystallization；But the technique waste water
Yield is more, and invests and land occupation is big, and needs a large amount of operator, and it is equal scarce that and process recovery ratio low there are working efficiency is low
It falls into.
Summary of the invention
In order to overcome the shortcomings of the prior art, the present invention proposes one kind separation and Extraction glucose from starch saccharificating liquid
Method.
To achieve the goals above, the present invention is achieved by the following technical solution:
A method of the separation and Extraction glucose from starch saccharificating liquid comprising following steps: step 1) preparation flocculation
Decolorising agent, step 2 filtering, step 3) flocculation decoloration, step 4) nanofiltration, step 5) crystallization, step 6) mother liquid disposal.
Specifically, described method includes following steps:
Step 1) prepares flocculating decoloring agent: (1) according to the mass ratio of 10:2:1 take chitosan, sodium humate and pectin,
Be mixed evenly, then addition account for twice of mass of chitosan 2M sodium hydrate aqueous solution, the ultrasonic 10min of 30KHz, most
500 turns/min stirs 3min afterwards, obtains modification of chitosan；(2) corn stover, sepiolite are crushed respectively and obtains corn stalk powder
And sepiolite powder, then corn stalk powder, sepiolite powder and diatomite are mixed evenly according to the mass ratio of 3:2:1,
It is added to the concentration for accounting for 5 times of weight of diatomite as in the sodium hydroxide solution of 1M, 200 turns/min stirs 120min, then filters,
It is dry, obtain mixed-powder；(3) modification of chitosan and mixed-powder are mixed evenly according to the mass ratio of 3:2, are put into
To pelletizer, it is granulated to obtain the final product；
Step 2 filtering: starch saccharificating liquid first passes through 1mm the screen to filtrate, after 40 mesh net filtrations, then through microfiltration membranes
Filtrate A is collected in filtering；
Step 3) flocculation decoloration: into filtrate A, addition accounts for filtrate A 0.3%(w/w) flocculating decoloring agent, 300 turns/min stirs
5min is mixed, 60min is then allowed to stand, 100 mesh net filtrations collect liquor B；
Step 4) nanofiltration: by liquor B by nanofiltration membrane, nanofiltration temperature is 35-40 DEG C, interception 200-300MW, is collected
Liquor C；
Step 5) crystallization: the half of liquor C concentration and evaporation to original volume, into crystallizer, crystallization, centrifuge separation is obtained
Obtain crystal and mother liquor；
Step 6) mother liquid disposal: mother liquor is drained into sedimentation basin, sediment and supernatant is obtained, supernatant is discharged into micro- life
Object reaction tank adds 10 grams of microbial bacterial agent by every cubic metre of supernatant every time, adds daily 1 time, continuously adds three days, finally
Four days are stood, liquid is discharged.
The microfiltration membranes are inorganic ceramic membrane, and molecular cut off 3000MW, micro-filtration temperature is 30-40 DEG C.
The microbial bacterial agent is prepared according to following technique: be first ATCC 23843 by bacillus amyloliquefaciens,
Rhodococcus sp ATCC 15906 and bacillus pumilus ATCC 27142 is cultivated respectively to concentration 2 × 108It is a/gram bacterium solution, so
Mixed bacteria liquid is mixed to get according to the volume ratio of 3:2:2 afterwards, stands 3 hours, then by mixed bacteria liquid and diatomite according to the matter of 1:2
Amount than mixing, it is dry to get.
Note, above-mentioned bacterial strains can be obtained from ATCC and the purchase of other commercial channel.
Compared with prior art, the advantages and positive effects of the present invention are:
The present invention uses flocculation purification decolorization, using multi-filtering step, including micro-filtration, nanofiltration etc., generates waste water
Few, investment and land occupation are also greatly reduced, and cost of material consumptive material is less expensive, are easy as technical staff's operation and yield and purity
It is high；The present invention provides a kind of novel flocculation decolorising agent, has macropore gauge structure, and adsorption capacity and specific surface area improve, can
Effectively adsorbed proteins, starch and small organic molecule pigment etc., and low raw-material cost；Microorganism formulation of the present invention is special
The various strains that can form dominant microflora are configured to high-efficiency microorganism preparation for the mother liquor waste water for extracting preparation process by door,
Reasonable compatibility, symbiosis are coordinated, mutually not antagonism, and activity is high, and biomass is big, and breeding is fast, and processing water and water quality treatment, drop can be improved
Low operating cost promotes qualified discharge；Flocculating decoloring agent waste of the present invention and the sediment being collected by filtration can answer
It uses in fertilizer preparation.
Specific embodiment
In order to make those skilled in the art better understand the technical solutions in the application, having below in conjunction with the application
Body embodiment more clearly and completely describes the present invention, it is clear that described embodiment is only the application one
Divide embodiment, instead of all the embodiments.Based on the embodiment in the application, those of ordinary skill in the art are not making
Every other embodiment obtained, should fall within the scope of the present invention under the premise of creative work.
Embodiment 1
A method of the separation and Extraction glucose from starch saccharificating liquid comprising following steps:
It prepares flocculating decoloring agent: (1) taking chitosan, sodium humate and pectin according to the mass ratio of 10:2:1, mixing is stirred
Mix uniformly, then addition account for twice of mass of chitosan 2M sodium hydrate aqueous solution, the ultrasonic 10min of 30KHz, last 500
Turn/min stirring 3min, obtains modification of chitosan；(2) corn stover, sepiolite are crushed respectively and obtains corn stalk powder and sea
Afrodite powder, then corn stalk powder, sepiolite powder and diatomite are mixed evenly according to the mass ratio of 3:2:1, are added to and account for
The concentration of 5 times of weight of diatomite is in the sodium hydroxide solution of 1M, and 200 turns/min stirs 120min, is then filtered, dry, is obtained
To mixed-powder；(3) modification of chitosan and mixed-powder are mixed evenly according to the mass ratio of 3:2, put into granulation
Machine is granulated to obtain the final product；Grain diameter is controlled in 300-600um；
Filtering: being prepared starch saccharificating liquid (pol 12%) using cornstarch enzyme process, and starch saccharificating liquid first passes through 1mm sieve
After net filtration, after 40 mesh screens, then through micro-filtrate membrane filtration, filtrate A is collected；The microfiltration membranes are inorganic ceramic membrane, retention
Molecular weight is 3000MW, and micro-filtration temperature is 30-40 DEG C；
Flocculation decoloration: into filtrate A addition account for filtrate A 0.3%(w/w) flocculating decoloring agent, 300 turns/min stirring
5min, is then allowed to stand 60min, and 100 mesh net filtrations collect liquor B；
Nanofiltration: by liquor B by nanofiltration membrane, nanofiltration temperature is 35-40 DEG C, nanofiltration pressure 15-20bar, and interception is
200-300MW collects liquor C；
Crystallization: the half of liquor C concentration and evaporation to original volume, into crystallizer, crystallization, centrifuge separation obtains crystal
And mother liquor；By the dry finished product of crystal；
Mother liquid disposal: mother liquor is drained into sedimentation basin, precipitating bottom of pond portion is the diatomite of 50cm thickness, natural subsidence solid-liquid
Separation obtains sediment and supernatant, supernatant is discharged into microbial reaction pond, adds micro- life every time by every cubic metre of supernatant
It 10 grams of object microbial inoculum, adds daily 1 time, continuously adds three days, finally stand four days, liquid is discharged.Through detecting: female before processing
COD in liquid, ammonia nitrogen concentration be respectively 1967mg/L and 179 mg/L, COD in liquid after processing, ammonia nitrogen concentration be respectively
103mg/L and 12 mg/L, removal rate reach 90% or more.
The microbial bacterial agent is prepared according to following technique:
It is first ATCC 23843, Rhodococcus sp ATCC 15906 and bacillus pumilus by bacillus amyloliquefaciens
ATCC 27142 is cultivated respectively to concentration 2 × 108It is a/gram bacterium solution, be then mixed to get Mixed Microbes according to the volume ratio of 3:2:2
Liquid stands 3 hours, then mixes mixed bacteria liquid and diatomite according to the mass ratio of 1:2, dry, holding moisture content is 20-
30wt%, packaging to get.
Embodiment 2
Glucose parametric measurement prepared by embodiment 1, is specifically shown in Table 1:
Table 1
| Index | Embodiment 1 | National standard |
| Purity % | 99.7 | ------ |
| Yield % | 83.2 | ------ |
| Acidity ml | 0.09 | Meet |
| Chloride % | Less than 0.003 | Meet |
| 25 DEG C of specific rotation | 7.6 | Meet |
| Heavy metal % | Less than 0.00002 | Meet |
Conclusion: the product purity and yield of separation and Extraction preparation of the present invention are good, and energy-saving and emission-reduction are environment friendly and pollution-free, preparation
Product meet National Pharmacopeia standard.
Listed above is only best specific embodiment of the invention.It is clear that the invention is not restricted to which above embodiments, may be used also
With there are many deformations.All changes that those skilled in the art directly can export or associate from present disclosure
Shape is considered as protection scope of the present invention.
Claims (2)
1. a kind of method of the separation and Extraction glucose from starch saccharificating liquid, which is characterized in that described method includes following steps:
Step 1) prepares flocculating decoloring agent: (1) taking chitosan, sodium humate and pectin according to the mass ratio of 10:2:1, mix
Stir evenly, then addition account for twice of mass of chitosan 2M sodium hydrate aqueous solution, the ultrasonic 10min of 30KHz, finally
500 turns/min stirs 3min, obtains modification of chitosan；(2) corn stover, sepiolite are crushed respectively obtain corn stalk powder and
Then corn stalk powder, sepiolite powder and diatomite are mixed evenly according to the mass ratio of 3:2:1, add by sepiolite powder
Being added to and accounting for the concentration of 5 times of weight of diatomite is in the sodium hydroxide solution of 1M, and 200 turns/min stirs 120min, then filters, and does
It is dry, obtain mixed-powder；(3) modification of chitosan and mixed-powder are mixed evenly according to the mass ratio of 3:2, are put into
Pelletizer is granulated to obtain the final product；
Step 2 filtering: starch saccharificating liquid first passes through 1mm the screen to filtrate, after 40 mesh net filtrations, then through micro-filtrate membrane filtration,
Collect filtrate A；
Step 3) flocculation decoloration: into filtrate A, addition accounts for the flocculating decoloring agent of filtrate A 0.3%w/w, 300 turns/min stirring
5min, is then allowed to stand 60min, and 100 mesh net filtrations collect liquor B；
Step 4) nanofiltration: by liquor B by nanofiltration membrane, nanofiltration temperature is 35-40 DEG C, interception 200-300MW, collects filtrate
C；
Step 5) crystallization: the half of liquor C concentration and evaporation to original volume, into crystallizer, crystallization, centrifuge separation obtains brilliant
Body and mother liquor；
Step 6) mother liquid disposal: mother liquor is drained into sedimentation basin, sediment and supernatant is obtained, it is anti-that supernatant is discharged into microorganism
Ying Chi adds 10 grams of microbial bacterial agent by every cubic metre of supernatant every time, adds daily 1 time, continuously adds three days, finally stand
Four days, liquid is discharged；
The microbial bacterial agent is prepared according to following technique: being first ATCC 23843, red ball by bacillus amyloliquefaciens
Bacterium ATCC 15906 and bacillus pumilus ATCC 27142 is cultivated respectively to concentration 2 × 108It is a/gram bacterium solution, then press
Mixed bacteria liquid is mixed to get according to the volume ratio of 3:2:2, stands 3 hours, then by mixed bacteria liquid and diatomite according to the mass ratio of 1:2
Mixing, it is dry.
2. molecular cut off is the method according to claim 1, wherein the microfiltration membranes are inorganic ceramic membrane
3000MW, micro-filtration temperature are 30-40 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610775617.XA CN106282422B (en) | 2016-08-31 | 2016-08-31 | A method of the separation and Extraction glucose from starch saccharificating liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610775617.XA CN106282422B (en) | 2016-08-31 | 2016-08-31 | A method of the separation and Extraction glucose from starch saccharificating liquid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106282422A CN106282422A (en) | 2017-01-04 |
| CN106282422B true CN106282422B (en) | 2019-07-05 |
Family
ID=57674133
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610775617.XA Active CN106282422B (en) | 2016-08-31 | 2016-08-31 | A method of the separation and Extraction glucose from starch saccharificating liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106282422B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109136305A (en) * | 2017-06-19 | 2019-01-04 | 卢松 | A kind of separation purifying technique of glucose |
| CN109136413A (en) * | 2017-06-19 | 2019-01-04 | 卢松 | A method of high purity glucose is prepared using cornstarch |
| CN108004190B (en) * | 2018-01-19 | 2019-12-06 | 杭州渔森农业技术开发有限公司 | Method for increasing chlorella biomass by using bacillus |
| CN110846359A (en) * | 2019-09-12 | 2020-02-28 | 赵兰坤 | Production method of edible glucose |
| CN112409423A (en) * | 2020-11-02 | 2021-02-26 | 呼伦贝尔东北阜丰生物科技有限公司 | Process for improving quality of glucose |
| CN112322679A (en) * | 2020-11-06 | 2021-02-05 | 齐齐哈尔龙江阜丰生物科技有限公司 | Novel saccharification process without cooling pipe |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0218010D0 (en) * | 2002-08-05 | 2002-09-11 | Ciba Spec Chem Water Treat Ltd | Production of a fermentation product |
| CN105821095A (en) * | 2015-09-14 | 2016-08-03 | 呼伦贝尔东北阜丰生物科技有限公司 | Optimization method for crystallization of glucose |
| CN105132593A (en) * | 2015-09-18 | 2015-12-09 | 山东创利添加剂研发科技有限公司 | Method for preparing crystalline fructose and crystal glucose from maize straw |
| CN105692823A (en) * | 2016-01-19 | 2016-06-22 | 赵东星 | Environment-friendly efficient biological flocculant and preparing method thereof |
-
2016
- 2016-08-31 CN CN201610775617.XA patent/CN106282422B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN106282422A (en) | 2017-01-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106282422B (en) | A method of the separation and Extraction glucose from starch saccharificating liquid | |
| CN106282423B (en) | A kind of technique that crystal glucose is extracted in recycling | |
| CN108864218B (en) | Glycerol glucoside product, purification method and application of glycerol glucoside | |
| US11555049B2 (en) | Method for separation and purification of n-acetylglucosamine | |
| CN106148445B (en) | A kind of new extraction technology of glutamic acid | |
| CN101100685B (en) | Method for preparing L-arabinose | |
| CN102242168B (en) | Process for producing high fructose syrup with content of 55% with rice | |
| CN106397630B (en) | A method of Sodium Hyaluronate is extracted using membrane separation technique | |
| CN101220381A (en) | Process for preparing xylitol by using corn core or agriculture and forestry castoff | |
| CN102965412A (en) | Trehalose preparation method | |
| CN102040638A (en) | Method for preparing nonsolvent of high-purity natamycin | |
| CN101434553B (en) | Method for all-film extraction of valine | |
| CN103755586B (en) | A kind of preparation method of L-glutaminate | |
| CN102532332A (en) | Method for extracting and preparing low molecular weight fucoidin from marine brown algae | |
| CN107722083A (en) | A kind of high-efficient production technology of stevioside | |
| CN104059169A (en) | Hyaluronic acid purification process | |
| CN109021095B (en) | A kind of high-purity is without fishy smell algae blue pigment and the preparation method and application thereof | |
| CN104059865A (en) | Streptococcus zooepidemicus and production process for preparing hyaluronic acid by using same | |
| CN102391317A (en) | Method for separating alginate-derived oligosaccharides from alginate-derived oligosaccharide fermentation liquor | |
| CN104055686B (en) | A kind of skin moisturizer | |
| CN110938713A (en) | Refining process of crystalline glucose | |
| CN1321126C (en) | Process for preparing high purity acarbose | |
| CN113754704A (en) | Preparation method for efficiently preparing glucose powder by using ionic resin | |
| CN103159643A (en) | Technology for whole membrane extraction of L-glutamine fermentation broth | |
| CN109422414B (en) | Environment-friendly process for treating glucose industrial wastewater |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |