CN106282309A - A kind of ADA Adenosine deaminase detection method - Google Patents
A kind of ADA Adenosine deaminase detection method Download PDFInfo
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Abstract
The invention discloses a kind of ADA Adenosine deaminase detection method.The detectable of adenosine deaminase is made up of following two parts: reagent 1 includes disodium hydrogen phosphate 8.5 12mmol/L；4 amino-antipyrine 1.5 2.2mmol/L；Purine nucleoside phosphorylase 2500 2900U/L；Xanthine oxidase 500 700U/L；Peroxidase 510 710U/L；Ascorbinase 1800 2800U/L；Stabilizer is appropriate；Cofactor is appropriate；Reagent 2 includes adenosine 10 14mmol/L；EHSPT 1.5‑2.5mmol/L.The detectable formula of adenosine deaminase of the present invention, and detect parameter and the determination of reference interval, beneficially the unification of Clinical detection result, offers convenience to clinic diagnosis.The inventive method sensitivity is higher, is better than conventional commercial product.
Description
Technical field
The invention belongs to field of medical examination, be specifically related to a kind of ADA Adenosine deaminase detection method.
Background technology
ADA Adenosine deaminase (ADA) is the nucleic acid decomposition metabolic enzyme that body is required, has important valency in clinical experiment diagnoses
Value.The continuous monitoring method using enzyme coupling principle of zymetology detection at present, measures the catalysis active concentration of enzyme, the method in sample more
There is the cold advantage of easy rapid nontoxic, extensively apply in clinic.Though the most clinical ADA detection method all uses four
The Cleaning Principle of step enzyme coupling, but spread in performance is uneven, and the agent prescription of different manufacturers and detection process all there are differences, no
Testing result between homologous ray differs greatly, and does not has unified reference interval, is unfavorable for the unification of Clinical detection result, gives
Clinic diagnosis brings many puzzlements.
Summary of the invention
It is an object of the invention to provide the detection method of a kind of ADA Adenosine deaminase.
The technical solution used in the present invention is:
The detectable of a kind of adenosine deaminase, is made up of following two parts:
Reagent 1
Reagent 2
Adenosine 10-14mmol/L；
EHSPT 1.5-2.5mmol/L。
Preferably, described adenosine deaminase detectable is made up of following two parts:
Reagent 1
Reagent 2
Adenosine 11-13mmol/L；
EHSPT 1.8-2.2mmol/L。
Preferably, described adenosine deaminase detectable is made up of following two parts:
Reagent 1
Reagent 2
Adenosine 12.2mmol/L；
EHSPT 2.0mmol/L。
Preferably, reagent 1 and reagent 2 are prepared by Tris buffer or PBS and are obtained.
Preferably, reagent 1 and reagent 2 are obtained by Tris buffer, and wherein the concentration of Tris buffer is 65-
95mmol/L, pH6-7.
Preferably, reagent 1 and reagent 2 are obtained by Tris buffer, and wherein the concentration of Tris buffer is 70-
90mmol/L, pH6.2-6.8.
Preferably, reagent 1 and reagent 2 are obtained by Tris-HCl buffer, wherein the concentration of Tris-HCl buffer
For 80.0mmol/L, pH6.6.
Tris buffer includes Tris-HCl and Tris-base.The buffer system of Tris is than PBS buffer system effect more
Good.It is still further preferred that Tris-HCl buffer, its concentration is 80.0mmol/L, pH6.6.
Preferably, stabilizer is selected from NaN3, its final concentration of 0.1-0.3mmol/L.
Preferably, stabilizer is selected from NaN3, its final concentration of 0.15-0.25mmol/L.
Preferably, stabilizer is selected from NaN3, its final concentration of 0.2mmol/L.
Preferably, cofactor is selected from TritonX-100, and its addition is the 0.6-0.9% of reagent 1 cumulative volume.
Preferably, cofactor is selected from TritonX-100, and its addition is the 0.7-0.8% of reagent 1 cumulative volume.
Preferably, cofactor is selected from TritonX-100, and its addition is the 0.76% of reagent 1 cumulative volume.
Cofactor TritonX-100, is also a kind of surfactant, and it can quickly eliminate nonspecific reaction, add
Fast response process, is the catalyst of whole reaction.Other surfactant such as tween etc. is unable to reach the effect of the present invention.
Preferably, reagent 1 is also added with mercapto-protective agent EDTA, its final concentration of 0.15-0.25mmol/L.
Preferably, reagent 1 is also added with mercapto-protective agent EDTA, its final concentration of 0.2mmol/L.EDTA can protect tested
The ADA protein active surveyed.
In above-mentioned reaction, disodium hydrogen phosphate can provide enough phosphate radicals, it is ensured that reaction is smoothed out.Purine nucleosides phosphorylation
Enzyme, xanthine oxidase, peroxidase are the toolenzymes of reaction coupling, by the intermediate reaction of these enzymes, make target enzyme
The product that reaction generates ultimately generates the coloring matter beneficially detected through serial reaction.4-AA and EHSPT are anti-
Answer the color reaction substrate of final step in principle.
The detection kit of a kind of ADA Adenosine deaminase, including the reagent described in any of the above-described item.
The detection method of a kind of ADA Adenosine deaminase, comprises the following steps:
1) by 180ul reagent 1 and 5ul test serum sample mix, hatch to 37 DEG C；
2) adding 90ul reagent 2 to detect, wherein agents useful for same is as described in above-mentioned any one.
Preferably, detection wavelength: 550 ± 1nm；Time delay: 120s；Monitoring time: 180s.
Preferably, detection reference interval is set up based on Chinese population, in the range from normal adults 0-31U/L.
The detection reference interval of detection method is set up based on Chinese population, has preferably adaptation to Chinese population
Property.
Preferably, the range of linearity is 0.3-286U/L.
Detection method has the wider range of linearity.
The invention has the beneficial effects as follows:
Testing conditions, detection process, on the Cleaning Principle of four step enzyme couplings, are researched and developed, are optimized by the inventive method
The optimum combination condition of the ADA detection arrived.
The detectable formula of adenosine deaminase of the present invention, detection parameter and the determination of reference interval, be conducive to clinic
The unification of testing result, offers convenience to clinic diagnosis.The inventive method sensitivity is higher, is better than conventional commercial product.
The inventive method detection sensitivity is greatly improved, and overcomes nonspecific reaction, and detection method is more stable, soon
Speed is the simplest, and performance obtains the biggest improvement, the most also establishes relatively full-page proof based on Chinese population on the basis of the method
This reference interval, has more preferable adaptability for Chinese population.
Accompanying drawing explanation
Fig. 1 is the omnidistance absorbance scanning figure of sample reaction；
Fig. 2 is the range of linearity of detection method；
Fig. 3 is reference interval histogram frequency distribution diagram；
Fig. 4 is optimization method and the dependency of mikey method and bias correspondence.
Detailed description of the invention
EHSPTN is writing a Chinese character in simplified form of N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline, in
Literary fame ethyl-N-(2-hydroxyl-3-sulfopropyl)-3-monomethylaniline..
The Cleaning Principle of the present invention, as follows:
Wherein, ADA: adenosine deaminase；PNP: purine nucleoside phosphorylase；XOD: for xanthine oxidase；POD: peroxide
Compound enzyme.
Below in conjunction with specific embodiment, the present invention is further illustrated, but is not limited thereto.
Embodiment 1
1, reagent composition and concentration
Reagent 1:Tris-HCl (80.0mmol/L, pH6.6), disodium hydrogen phosphate (Na2HPO4, 10.3mmol/L), 4-amino
Phenazone (4-AA, 1.8mmol/L), purine nucleoside phosphorylase (PNP, 37 DEG C of 2760U/L), xanthine oxidase (XOD,
37 DEG C of 600U/L), peroxidase (POD, 37 DEG C of 590U/L), ascorbinase (37 DEG C of 2306U/L), NaN3(0.2mmol/
L), TritonX-100 (the 0.76% of reagent 1 cumulative volume), EDTA (0.2mmol/L)；
Reagent 2 (beginning reagent): Tris-HCl (80.0mmol/L, pH6.6), adenosine (12.2mmol/L), EHSPT
(2.0mmol/L)。
2, testing conditions
Detection temperature: 37 ± 0.1 DEG C；Detection wavelength: 550 ± 1nm；Ripple width: 10mm ± 0.01mm, optical path≤2nm, detection
Point >=6；Incubation time: 300s；Time delay: 120s；Monitoring time: 180s.
3, detection sample: serum.
4, the detection method of a kind of ADA Adenosine deaminase, comprises the following steps:
1) 180ul reagent 1 and 5ul serum sample adds reaction cup, blending incubation to 37 DEG C；
Part instrument can stir to heat and carry out simultaneously；If substep carry out, the most first mix, after hatch to 37 DEG C, temperature
Reach 37 DEG C, about need 300s.
2) add 90ul reagent 2, postpone 120s, monitor the linear of other 180s；Wherein, available biochemistry analyzer is automatic
Detect or utilize ultraviolet spectrophotometer to carry out manual inspection.The omnidistance absorbance scanning figure of sample reaction is shown in Fig. 1.
Fig. 1 explanation is hatched the stage at sample and reactant liquor, and curve is straight (first 300 seconds) illustrates without non-specific (interference) instead
Should, add after starting reagent, reaction is linearly.
3) ADA is calculated active: ADA (U/L)=F* Δ A/ Δ t550；
Conversion factor F=1708 [at ε (the trophicardyl)=32.2mmol/cm of 550nm].
The performance parameter of embodiment 2 ADA Adenosine deaminase detection
The performance parameter of this technology is determined according to the method for embodiment 1.
Result is as follows:
Withinrun precision :≤1%, period precision :≤2%；
The range of linearity: 0.3-286U/L；
Sensitivity: examination criteria species activity is beyond given target value about 30%.
Capacity of resisting disturbance: triglyceride≤3.39mmol/L (about 20 times of adult normal's levels), hemoglobin≤3.75g/L
(macroscopic haemolysis is 300mg/dl), (normal human serum dimension is raw for bilirubin ＜ 85.5umol/L, vitamin C≤40mg/L
The content of element C is ng/dl rank), enoxolone and deoxycholic acid≤20mg/L, to method without dry during baicalin≤37.5mg/L
Disturb.
The range of linearity figure of detection method is shown in accompanying drawing 2.The linear coefficient of Fig. 2 curve is notable, coefficient R2=0.9998
Show that X span is suitable, in the range of 0-286U/L linearly.
Detection method has wider range of linearity 0.3-286U/L, is better than commercially available prod on the market.
Embodiment 3 replica test
The method set up the present invention below carries out repeatability detection, and calculates withinrun precision and period precision respectively
Degree.
Have chosen and be visible by naked eyes the serum sample (just two concentration) that lipidemia, haemolysis, jaundice and infectiousness index are negative.
Withinrun precision: every sample continuous detecting 10 times in a batch, calculates average, standard deviation and the coefficient of variation (CV), period essence
Density is: every sample detects 3 times every day, and continuous detecting 12 days calculates average, standard deviation and the coefficient of variation (CV).
Result is withinrun precision :≤1%, period precision :≤2%.
Criticizing of illustration method is interior preferable with period repeatability.
The reference interval of embodiment 4 ADA Adenosine deaminase testing result
Select the healthy volunteer of 18-79 one full year of life (man, female), detect according to the method for embodiment 1.The most always volunteer
Person's n=1662 name, includes experimental subjects n=742 name in, and test in laboratory gets rid of individual n=92 name, includes the individual n=650 of statistics in
Name, wherein man's n=296 name, female's n=354 name.Use nonparametric statistical method, calculate frequency distribution 95% credibility interval and
90% with reference to the confidence interval limited, and sample distribution median is 16.4U/L, and average is 17.3U/L, and the standard deviation of average is
5.2U/L, standard is mistaken for 0.2U/L, minima 4.2U/L, maximum 43.5U/L, 95% (one-sided test, α=0.05) reference area
Between be 0-31U/L, the confidence interval of 90% reference upper level is 30.5-31.1U/L.Fig. 3 is shown in pattern detection result frequency distribution.
Fig. 3 is reference interval histogram frequency distribution diagram；Testing result frequency distribution, substantially in normal state, illustrates that sample size is enough
And data are effective, subsequent calculations can be carried out.
The detection reference interval of detection method is set up based on Chinese population, has preferably adaptation to Chinese population
Property, in the range from normal adults 0-31U/L.
Comparative example 1
The present invention and Sichuan mikey ADA Adenosine deaminase (ADA) measure test kit compare experiment.Result is shown in Fig. 4.
Fig. 4 A shows this method and the linearly positive correlation of mikey method.It is negative that Fig. 4 B shows that mikey method is relative to this method
Skew, illustrate with favourable conditions than mikey method of this method testing conditions, raising detection sensitivity.The inventive method ADA detects
Specific activity mikey kit method result improves about 30-60%.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention are not by above-described embodiment
Limit, the change made under other any spirit without departing from the present invention and principle, modify, substitute, combine, simplify,
All should be the substitute mode of equivalence, within being included in protection scope of the present invention.
Claims (10)
1. a detectable for adenosine deaminase, is made up of following two parts:
Reagent 1
Disodium hydrogen phosphate 8.5-12mmol/L；
4-AA 1.5-2.2mmol/L；
Purine nucleoside phosphorylase 2500-2900U/L；
Xanthine oxidase 500-700U/L；
Peroxidase 510-710U/L；
Ascorbinase 1800-2800U/L；
Stabilizer is appropriate；
Cofactor is appropriate；
Reagent 2
Adenosine 10-14mmol/L；
EHSPT 1.5-2.5 mmol/L。
Detectable the most according to claim 1, it is characterised in that: it is made up of following two parts:
Reagent 1
Disodium hydrogen phosphate 9-11mmol/L；
4-AA 1.6-2.0mmol/L；
Purine nucleoside phosphorylase 2600-2800U/L；
Xanthine oxidase 550-650U/L；
Peroxidase 560-660U/L；
Ascorbinase 2000-2500U/L；
Stabilizer is appropriate；
Cofactor is appropriate；
Reagent 2
Adenosine 11-13mmol/L；
EHSPT 1.8-2.2 mmol/L。
Detectable the most according to claim 1, it is characterised in that: it is made up of following two parts:
Reagent 1
Disodium hydrogen phosphate 10.3mmol/L；
4-AA 1.8mmol/L；
Purine nucleoside phosphorylase 2760U/L；
Xanthine oxidase 600U/L；
Peroxidase 590U/L；
Ascorbinase 2306U/L；
Stabilizer is appropriate；
Cofactor is appropriate；
Reagent 2
Adenosine 12.2mmol/L；
EHSPT 2.0 mmol/L。
4. according to the detectable described in any one of claim 1-3, it is characterised in that: reagent 1 and reagent 2 are buffered by Tris
Liquid or PBS preparation obtain.
5. according to the detectable described in any one of claim 1-3, it is characterised in that: reagent 1 and reagent 2 are by Tris buffer
Preparation obtains, and wherein the concentration of Tris buffer is 65-95 mmol/L, pH6-7.
6. according to the detectable described in any one of claim 1-3, it is characterised in that: stabilizer is selected from NaN3, it is final concentration of
0.1-0.3mmol/L。
7. according to the detectable described in any one of claim 1-3, it is characterised in that: cofactor is selected from TritonX-100,
Its addition is the 0.6-0.9% of reagent 1 cumulative volume.
8. according to the detectable described in any one of claim 1-3, it is characterised in that: reagent 1 is also added with mercapto-protective agent
EDTA, its final concentration of 0.15-0.25 mmol/L.
9. a detection kit for ADA Adenosine deaminase, including the reagent described in any one of claim 1-8.
10. the detection method of an ADA Adenosine deaminase, it is characterised in that comprise the following steps:
1) by 180ul reagent 1 and 5ul test serum sample mix, hatch to 37 DEG C；
2) adding 90ul reagent 2 to detect, wherein agents useful for same is as described in any one of claim 1-8.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107653298A (en) * | 2017-11-15 | 2018-02-02 | 浙江夸克生物科技有限公司 | Adenosine deaminase determines kit |
| WO2022155991A1 (en) * | 2021-01-19 | 2022-07-28 | 苏州凯祥生物科技有限公司 | Kit for detecting purine substances and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102586397A (en) * | 2011-01-11 | 2012-07-18 | 上海复星长征医学科学有限公司 | Enzymatic detection adenosine deaminase kit |
| CN102703576A (en) * | 2012-05-24 | 2012-10-03 | 宁波美康生物科技股份有限公司 | Determination method for S-adenosylmethionine methyltransferase and kit thereof |
| CN105238847A (en) * | 2015-09-16 | 2016-01-13 | 山东博科生物产业有限公司 | Stable adenosine deaminase reagent high in anti-interference capability and detection method |
-
2016
- 2016-08-31 CN CN201610798128.6A patent/CN106282309A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102586397A (en) * | 2011-01-11 | 2012-07-18 | 上海复星长征医学科学有限公司 | Enzymatic detection adenosine deaminase kit |
| CN102703576A (en) * | 2012-05-24 | 2012-10-03 | 宁波美康生物科技股份有限公司 | Determination method for S-adenosylmethionine methyltransferase and kit thereof |
| CN105238847A (en) * | 2015-09-16 | 2016-01-13 | 山东博科生物产业有限公司 | Stable adenosine deaminase reagent high in anti-interference capability and detection method |
Non-Patent Citations (2)
| Title |
|---|
| DIAZYME LABORATORIES: "Adenosine Deaminase assay kit", 《WWW.DIAZYME.COM》 * |
| 黄雪丽: "血清腺苷脱氨酶匀相法检测新技术的研究", 《万方数据知识服务平台》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107653298A (en) * | 2017-11-15 | 2018-02-02 | 浙江夸克生物科技有限公司 | Adenosine deaminase determines kit |
| WO2022155991A1 (en) * | 2021-01-19 | 2022-07-28 | 苏州凯祥生物科技有限公司 | Kit for detecting purine substances and application thereof |
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Inventor after: Huang Xianzhang Inventor after: Han Liqiao Inventor after: Zhuang Junhua Inventor after: Yuan Lin Inventor after: Shi Wen Inventor after: Zhang Qiaoxuan Inventor after: Wang Jianbing Inventor after: Lin Haibiao Inventor before: Huang Xianzhang Inventor before: Han Liqiao Inventor before: Zhuang Junhua Inventor before: Wang Jianbing Inventor before: Lin Haibiao Inventor before: Zhang Qiaoxuan |
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