CN106279497A - 一种pH敏感性葡萄糖印迹微凝胶的制备方法 - Google Patents

一种pH敏感性葡萄糖印迹微凝胶的制备方法 Download PDF

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CN106279497A
CN106279497A CN201610655615.7A CN201610655615A CN106279497A CN 106279497 A CN106279497 A CN 106279497A CN 201610655615 A CN201610655615 A CN 201610655615A CN 106279497 A CN106279497 A CN 106279497A
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潘忠宁
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Abstract

一种pH敏感性葡萄糖印迹微凝胶的制备方法，将1‑2份MAA、1份MMA、1份HEMA、0.6‑1份EGDMA和4份葡萄糖于烧杯中，静置混合30min，再加入0.5‑0.8份的SDS和200ml的水，将上述溶液转移至三口烧瓶中，40℃水浴搅拌溶解，通氮气30min，再加入0.8‑1.0份的APS，同时升温到75‑80℃，恒温搅拌反应5.5‑6ｈ，生成微凝胶,将产品离心，过滤后，用洗脱液反复洗涤，再离心，过滤，然后冷冻干燥，即得。本发明的有益效果是：以甲基丙烯酸酯类单体构建分子印迹体系，以葡萄糖为模板分子，制备对葡萄糖具有识别性的pH敏感性分子印迹微凝胶。

Description

一种pH敏感性葡萄糖印迹微凝胶的制备方法
技术领域
本发明涉及材料合成方法，具体的是一种pH敏感性葡萄糖印迹微凝胶的制备方法。
背景技术
20世纪50年代， Hirshbergt等提出光致变色的概念，指出光成色与光漂白的循环可构成化学记忆模型，从而使光致变色材料用于光信息存储。光致变色反应材料是一个依靠光激发来改变吸收性质的材料，在激发撤除以后，诱导变色能力仍能保留。因此能在热作用条件下或在相应诱导吸收的光激发下返回原始状态。同时，光致变色材料具有可逆的行为，可以导致颜色的改变。 变色材料种类较多，结构也千差万别，有无机材料也有有机材料。有机光致变色化合物种类较多，如俘精酸酐类化合物、二芳基乙烯类化合物、席夫碱类化合物、吡喃类化合物以及螺吡喃和螺噁嗪类化合物等。有些有机光致材料已经开始了工业化。本研究以吲哚啉为母体设计合成了一种新型光致变色材料。
发明内容
本发明所要解决的技术问题在于提供一种pH敏感性葡萄糖印迹微凝胶的制备方法，提供一种新的合成方法。
本发明采用的合成方法，包括如下步骤：
将1-2份MAA、1份MMA、1份HEMA、0.6-1份EGDMA和4份葡萄糖于烧杯中，静置混合30min，再加入0.5-0.8份的SDS和200ml的水，将上述溶液转移至三口烧瓶中，40℃水浴搅拌溶解，通氮气30min，再加入0.8-1.0份的APS，同时升温到75-80℃，恒温搅拌反应5.5-6ｈ，生成微凝胶,将产品离心，过滤后，用洗脱液反复洗涤，再离心，过滤，然后冷冻干燥，即得。
本发明的有益效果是：合成工艺简单，反应条件温和，生产成本较低，可重复性好究，以甲基丙烯酸酯类单体构建分子印迹体系，以葡萄糖为模板分子，制备对葡萄糖具有识别性的pH敏感性分子印迹微凝胶。
附图说明
图1是微凝胶的溶胀度。
具体实施方式
以下结合实例进一步说明本发明的内容，由技术常识可知，本发明也可通过其它的不脱离本发明技术特征的方案来描述，因此所有在本发明范围内或等同本发明范围内的改变均被本发明包含。
实施例1：将1份MAA、1份MMA、1份HEMA、0.6份EGDMA和4份葡萄糖于烧杯中，静置混合30min，再加入0.5份的SDS和200ml的水，将上述溶液转移至三口烧瓶中，40℃水浴搅拌溶解，通氮气30min，再加入0.8份的APS，同时升温到75℃，恒温搅拌反应5.5ｈ，生成微凝胶,将产品离心，过滤后，用洗脱液反复洗涤，再离心，过滤，然后冷冻干燥，即得。
实施例2：将2份MAA、1份MMA、1份HEMA、1份EGDMA和4份葡萄糖于烧杯中，静置混合30min，再加入0.8份的SDS和200ml的水，将上述溶液转移至三口烧瓶中，40℃水浴搅拌溶解，通氮气30min，再加入1份的APS，同时升温到80℃，恒温搅拌反应6ｈ，生成微凝胶,将产品离心，过滤后，用洗脱液反复洗涤，再离心，过滤，然后冷冻干燥，即得。
由图1可看出，微凝胶的溶胀度在pH为４～６间有一个阶跃性增大，显示出微凝胶具有明显的pH敏感性，
以与葡萄糖结构类似的果糖作为竞争底物，葡萄糖印迹微凝胶的选择吸附实验结果，印迹微凝胶对葡萄糖及果糖的吸附量差别较大，分离因子α为2.05。

Claims (1)

1.一种pH敏感性葡萄糖印迹微凝胶的制备方法，其特征在于：将1-2份MAA、1份MMA、1份HEMA、0.6-1份EGDMA和4份葡萄糖于烧杯中，静置混合30min，再加入0.5-0.8份的SDS和200ml的水，将上述溶液转移至三口烧瓶中，40℃水浴搅拌溶解，通氮气30min，再加入0.8-1.0份的APS，同时升温到75-80℃，恒温搅拌反应5.5-6ｈ，生成微凝胶,将产品离心，过滤后，用洗脱液反复洗涤，再离心，过滤，然后冷冻干燥，即得。
CN201610655615.7A 2016-08-11 2016-08-11 一种pH敏感性葡萄糖印迹微凝胶的制备方法 Pending CN106279497A (zh)

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104725556A (zh) * 2015-04-08 2015-06-24 福州大学 一种对pH敏感的葡萄糖选择吸附剂及其制备方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104725556A (zh) * 2015-04-08 2015-06-24 福州大学 一种对pH敏感的葡萄糖选择吸附剂及其制备方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
薛飞等: "葡萄糖检测用分子印迹光子晶体的研究", 《分析化学研究报告》 *

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