CN106279454A - The physical property method of modifying of a kind of soluble soybean polysaccharide and preparation - Google Patents
The physical property method of modifying of a kind of soluble soybean polysaccharide and preparation Download PDFInfo
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Abstract
nullThe invention discloses a kind of soluble soybean polysaccharide physical property method of modifying and preparation thereof，Belong to agricultural byproducts processing field,Octenyl succinic acid anhydride and soybean polysaccharide generation chemical action is particularly utilized to obtain having the reaction technology of novel soybean polysaccharide of hydrophilic-hydrophobic amphipathic characteristic,First with hexametaphosphate as cross-linking agent by soluble soybean polysaccharide cross-linked esterification，Then utilize octenyl succinic acid anhydride (OSA) that crosslinking soybean polysaccharide is carried out hydrophobic modification reaction under the conditions of pH8.0-8.5，Ocentyl succinic-soybean polysaccharide ester (OSA-SSPS) crude product will be obtained extract through ethyl acetate，Rotary evaporation lyophilization or pulverize after being spray-dried gets product,Soybean polysaccharide after suitably physical property is modified has hydrophilic and hydrophobic two kinds of surface naturies simultaneously，It is more suitable for playing the stability property of sterically hindered effect.The present invention can widen its range of application further, is widely used in food, cosmetics, weaving, papermaking, pharmaceuticals industry.
Description
Technical field
The invention discloses a kind of soluble soybean polysaccharide physical property method of modifying and preparation thereof, particularly soluble soybean polysaccharide is carried out cross-linked esterification molecular modification, and utilizes octenyl succinic acid anhydride and soybean polysaccharide generation chemical action to obtain the reaction technology of octenyl succinic acid anhydride-soybean polysaccharide ester (OSA-SSPS).Belong to agricultural byproducts processing field.
Background technology
Soluble soy protein (Soybean soluble polysaccharides, SSPS) is the dietary fiber of a kind of solubility extracted from soybean residue, has multiple application function, can be as emulsifying agent, dispersant, stabilizer etc..In milk product, soluble soybean polysaccharide relies primarily on the sterically hindered effect of polysaccharide side chain formation and makes protein stabilization, is a kind of low viscous sour milk beverage stabilizer.In recent years, soybean polysaccharide application in acidic milk drink gets more and more, and compared with the stabilizer improving stability with increase viscosity that in milk product, other is conventional, using soluble soybean polysaccharide as the milk beverage clean taste of stabilizer, local flavor is natural, viscosity is low.
Acidic dairy prod industry in the market has become ripe, through years of researches and production practices, in the acidic dairy prod that protein content is 1%, add 0.4% pectin have become as a kind of usage of trade as stabilizer, because 0.4% pectin can stable acidic milk beverage well.But pectin dealing in recent years is well sold and in short supply, and supply falls short of demand, price continuous rise, and the profit margin of businessman is more and more less.The challenge of this industry faces innovation.The commercially produced product of soluble soybean polysaccharide at present, the ability stablizing lactoprotein granule in the acidic dairy prod that protein content is 1% is not ideal；Additionally, in emulsion system, the soluble soybean polysaccharide of commercially produced product can not be formed as stabilizer and well stablize O/W emulsion system.In terms of soluble soybean polysaccharide modification, there is the research using expanded, extruding, enzyme process etc. that fiber is modified both at home and abroad, improve the functional characteristic of soybean polysaccharide.
Soluble soybean polysaccharide relies primarily on the sterically hindered effect of tral sugar side chains to realize the use value of its functional stabilizer, but soybean polysaccharide molecular weight, chain length is shorter, sterically hindered limited use, by esterification and crosslinking reaction modifying soybean polysaccharide, making its side chain increase, sterically hindered effect strengthens, to improving its emulsion stability；The existing hydrophobic chain alkyl of octenyl succinic acid anhydride (OSA) simultaneously, responds again activity well hydrophilic anhydride group.Many scholars are based on its amphiphatic character, octenyl succinic starch ester is prepared through esterification with corn starch, tapioca, potato starch etc., product is made to have hydrophilic and hydrophobic character, it is a kind of edible emulsifing thickener high by the safety of the major country approval use of the U.S., Europe and the Asian-Pacific area, is widely used in the industries such as food, medicine, cosmetics, chemical industry.Therefore, the present invention is reacted by cross-linked esterification and OSA suitably modifies soluble soybean polysaccharide, solve soluble soybean polysaccharide well and be applied to the problems such as medium-term and long-term float, precipitate or the layering placing generation of acidic milk drink, soybean polysaccharide after physical property is modified has hydrophilic and hydrophobic two kinds of surface naturies simultaneously, is more suitable for playing the stable essence of sterically hindered effect；By the modification to soybean polysaccharide molecular side chain, strengthen its sterically hindered effect, then can improve its dispersion stabilization further.This technology can widen its range of application further, is widely used in food, cosmetics, weaving, papermaking, pharmaceuticals industry.Low in raw material price used by the present invention, reagent and method safety, economical, practical, industrialized production is simple and easy to control, and product has the ability preferably stablizing lactoprotein particle.
Summary of the invention
The present invention solves soluble soybean polysaccharide soybean polysaccharide molecular weight, and chain length is shorter, sterically hindered limited use, the problem stablizing lactoprotein particle ability, it is proposed that a kind of soluble soybean polysaccharide physical property method of modifying.
The technical scheme that the present invention provides, a kind of soluble soybean polysaccharide physical property method of modifying and preparation: soluble soybean polysaccharide is carried out cross-linked esterification molecular modification, and utilizes octenyl succinic acid anhydride and soybean polysaccharide generation chemical action to obtain the reaction technology of octenyl succinic acid anhydride-soybean polysaccharide ester (OSA-SSPS).Specifically comprise the following steps that
(1) cross-linked esterification reaction: soluble soybean polysaccharide water is modulated into the solution that concentration is mass fraction 5-10%, add and account for the hexametaphosphate of soybean polysaccharide quality 5%-10% as cross-linking agent, add the hydrochloric acid solution regulation pH to 5.0-5.5 that concentration is 5% mass fraction, under room temperature, stir 0.5-1h.
(2) hydrophobic modification reaction: the crosslinking soybean polysaccharide solution of step (1) is positioned in 35-40 DEG C of water-bath, and under conditions of auger, add the sodium hydroxide solution that concentration is 5% mass fraction, make pH in the scope of 8.0～8.5, the most dropwise drip octenyl succinic acid anhydride (OSA) 5-25ml.In course of reaction, the pH controlling reaction system with the sodium hydroxide solution that concentration is 5% mass fraction is maintained between 8.0～8.5, reacts 30-60 min.Being 5.5-6.5 with the pH of the hydrochloric acid solution regulation solution that concentration is 5% mass fraction, rotary evaporation in vacuo concentrates, and obtains ocentyl succinic-soybean polysaccharide ester (OSA-SSPS) crude product.
(3) ethyl acetate processes: extract three times by isopyknic ethyl acetate, and rotary evaporation is pulverized after removing the ethyl acetate of residual, lyophilization or spray drying and got product.
2. according to physical property method of modifying and the preparation of soluble soybean polysaccharide a kind of described in claim 1, it is characterized in that: step (1) described cross-linked esterification reaction condition is: soluble soybean polysaccharide water is modulated into the solution that concentration is mass fraction 10%, add and account for the hexametaphosphate of soybean polysaccharide quality 6% as cross-linking agent, regulation pH to 5.0, stirs 0.5-1h at 25 DEG C.
3 according to the physical property method of modifying of soluble soybean polysaccharide a kind of described in claim 1 and preparation, it is characterized in that: step (2) described hydrophobic modification reaction condition is: by the crosslinking soybean polysaccharide solution of step (1) under the conditions of 35-40 DEG C, auger dropwise drips octenyl succinic acid anhydride (OSA) 15ml, react 45 min, in course of reaction, the pH controlling reaction system with the sodium hydroxide solution that concentration is 5% mass fraction is maintained between 8.0～8.5.With the HCL solution regulation pH 6.0 that concentration is 5% mass fraction after reaction, rotary evaporation in vacuo concentrates, obtains ocentyl succinic-soybean polysaccharide ester (OSA-SSPS) crude product.
4 according to the physical property method of modifying of soluble soybean polysaccharide a kind of described in claim 1 and preparation, it is characterised in that: the hexametaphosphate described in step (1) is sodium hexameta phosphate.
5 according to the physical property method of modifying of soluble soybean polysaccharide a kind of described in claim 1 and preparation, it is characterised in that: the room temperature described in step (1) is 20-30 DEG C.
The OSA-SSPS substitution value prepared by the inventive method is 0.0059-0.0206.
Beneficial effects of the present invention: the inventive method proposes first and first soluble soybean polysaccharide carried out cross-linked esterification molecular modification, further utilize octenyl succinic acid anhydride and soybean polysaccharide generation chemical action to obtain the reaction technology of octenyl succinic acid anhydride-soybean polysaccharide ester (OSA-SSPS), be applied in the production of soluble soybean polysaccharide.Reacted by cross-linked esterification and OSA suitably modify after soluble soybean polysaccharide, soybean polysaccharide has hydrophilic and hydrophobic two kinds of surface naturies simultaneously, is more suitable for playing the stable essence of sterically hindered effect；By the modification to soybean polysaccharide molecular side chain, strengthen its sterically hindered effect, then can improve its dispersion stabilization further.This technology can widen its range of application further, is widely used in food, cosmetics, weaving, papermaking, pharmaceuticals industry.Low in raw material price used by the present invention, reagent and method safety, economical, practical, industrialized production is simple and easy to control, it is easy to accomplish.
Detailed description of the invention
Embodiment 1
Material: soluble soybean polysaccharide (SSPS) derives from Fujian Wei Bo Food Co., Ltd.
Soluble soybean polysaccharide water is modulated into the solution that concentration is mass fraction 5%, adds the hexametaphosphate accounting for soybean polysaccharide quality 5% as cross-linking agent, at regulation pH value of solution 5.0,25 DEG C, stir 0.5h；After crosslinking soybean polysaccharide solution is positioned in 35 DEG C of water-baths, the scope that pH is 8.0～8.5 is adjusted under conditions of auger, the most dropwise drip octenyl succinic acid anhydride (OSA) 5ml, drip in 30min, in course of reaction between maintenance system pH 8.0～8.5.After having reacted with immediately adjust system pH be 5.5, rotary evaporation in vacuo concentrate, obtain ocentyl succinic-soybean polysaccharide ester (OSA-SSPS) crude product.Extracting three times by isopyknic ethyl acetate, rotary evaporation removes the ethyl acetate of residual, pulverizes and get product after lyophilization.After measured, its substitution value is 0.0059.
Embodiment 2
Soluble soybean polysaccharide water is modulated into the solution that concentration is mass fraction 10%, adds the hexametaphosphate accounting for soybean polysaccharide quality 8% as cross-linking agent, at regulation pH value of solution 5.2,20 DEG C, stir 1h；After crosslinking soybean polysaccharide solution is positioned in 40 DEG C of water-baths, the scope that pH is 8.0～8.5 is adjusted under conditions of auger, the most dropwise drip octenyl succinic acid anhydride (OSA) 25ml, drip in 60 min, in course of reaction between maintenance system pH 8.0～8.5.After having reacted with immediately adjust system pH be 6.0, rotary evaporation in vacuo concentrate, obtain ocentyl succinic-soybean polysaccharide ester (OSA-SSPS) crude product.Extracting three times by isopyknic ethyl acetate, rotary evaporation removes the ethyl acetate of residual, pulverizes and get product after spray drying.After measured, its substitution value is 0.0206.
Embodiment 3
Soluble soybean polysaccharide water is modulated into the solution that concentration is mass fraction 8%, adds the hexametaphosphate accounting for soybean polysaccharide quality 6% as cross-linking agent, at regulation pH value of solution 5.5,30 DEG C, stir 45min；After crosslinking soybean polysaccharide solution is positioned in 37 DEG C of water-baths, the scope that pH is 8.0～8.5 is adjusted under conditions of auger, the most dropwise drip octenyl succinic acid anhydride (OSA) 15 ml, drip in 45min, in course of reaction between maintenance system pH 8.0～8.5.After having reacted with immediately adjust system pH be 6.5, rotary evaporation in vacuo concentrate, obtain ocentyl succinic-soybean polysaccharide ester (OSA-SSPS) crude product.Extracting three times by isopyknic ethyl acetate, rotary evaporation removes the ethyl acetate of residual, pulverizes and get product after lyophilization.After measured, its substitution value is 0.0083.
Embodiment 4
Soluble soybean polysaccharide water is modulated into the solution that concentration is mass fraction 6%, adds the hexametaphosphate accounting for soybean polysaccharide quality 10% as cross-linking agent, at regulation pH value of solution 5.0,30 DEG C, stir 0.5h；After crosslinking soybean polysaccharide solution is positioned in 40 DEG C of water-baths, the scope that pH is 8.0～8.5 is adjusted under conditions of auger, the most dropwise drip octenyl succinic acid anhydride (OSA) 20 ml, drip in 45min, in course of reaction between maintenance system pH 8.0～8.5.After having reacted with immediately adjust system pH be 6.0, rotary evaporation in vacuo concentrate, obtain ocentyl succinic-soybean polysaccharide ester (OSA-SSPS) crude product.Extracting three times by isopyknic ethyl acetate, rotary evaporation removes the ethyl acetate of residual, pulverizes and get product after spray drying.After measured, its substitution value is 0.0125.
Claims (5)
1. the physical property method of modifying of a soluble soybean polysaccharide and preparation, it is characterized in that: soluble soybean polysaccharide is carried out cross-linked esterification molecular modification, and utilize octenyl succinic acid anhydride and soybean polysaccharide generation chemical action to obtain the reaction technology of octenyl succinic acid anhydride-soybean polysaccharide ester (OSA-SSPS), specifically comprise the following steps that
(1) cross-linked esterification reaction: soluble soybean polysaccharide water is modulated into the solution that concentration is mass fraction 5-10%, add and account for the hexametaphosphate of soybean polysaccharide quality 5%-10% as cross-linking agent, add the hydrochloric acid solution regulation pH to 5.0-5.5 that concentration is 5% mass fraction, under room temperature, stir 0.5-1h;
(2) hydrophobic modification reaction: the crosslinking soybean polysaccharide solution of step (1) is positioned in 35-40 DEG C of water-bath, and under conditions of auger, add the sodium hydroxide solution that concentration is 5% mass fraction, make pH 8.0～8.5
Scope, the most dropwise drip octenyl succinic acid anhydride (OSA) 5-25ml, in course of reaction, control the pH of reaction system with sodium hydroxide solution that concentration is 5% mass fraction
It is maintained at 8.0～8.5
Between, reacting 30-60 min, be 5.5-6.5 with the pH of the hydrochloric acid solution regulation solution that concentration is 5% mass fraction, rotary evaporation in vacuo concentrates, and obtains ocentyl succinic-soybean polysaccharide ester (OSA-SSPS) crude product;
(3) ethyl acetate processes: extract three times by isopyknic ethyl acetate, and rotary evaporation is pulverized after removing the ethyl acetate of residual, lyophilization or spray drying and got product.
2. according to physical property method of modifying and the preparation of soluble soybean polysaccharide a kind of described in claim 1, it is characterized in that: step (1) described cross-linked esterification reaction condition is: soluble soybean polysaccharide water is modulated into the solution that concentration is mass fraction 10%, add and account for the hexametaphosphate of soybean polysaccharide quality 6% as cross-linking agent, regulation pH to 5.0, stirs 0.5-1h at 25 DEG C.
3. according to physical property method of modifying and the preparation of soluble soybean polysaccharide a kind of described in claim 1, it is characterized in that: step (2) described hydrophobic modification reaction condition is: by the crosslinking soybean polysaccharide solution of step (1) under the conditions of 35-40 DEG C, auger dropwise drips octenyl succinic acid anhydride (OSA) 15ml, react 45 min, in course of reaction, the pH controlling reaction system with the sodium hydroxide solution that concentration is 5% mass fraction is maintained at
Between 8.0～8.5, with the HCL solution regulation pH 6.0 that concentration is 5% mass fraction after reaction, rotary evaporation in vacuo concentrates, obtains ocentyl succinic-soybean polysaccharide ester (OSA-SSPS) crude product.
4. according to physical property method of modifying and the preparation of soluble soybean polysaccharide a kind of described in claim 1, it is characterised in that: the hexametaphosphate described in step (1) is sodium hexameta phosphate.
5. according to physical property method of modifying and the preparation of soluble soybean polysaccharide a kind of described in claim 1, it is characterised in that: the room temperature described in step (1) is 20-30 DEG C.
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Cited By (7)
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| CN107232353A (en) * | 2017-05-31 | 2017-10-10 | 北京佑众全椒制药有限公司 | A kind of preparation method of clearing heat and detoxicating chrysanthemum beverage |
| CN107296125A (en) * | 2017-05-31 | 2017-10-27 | 北京佑众全椒制药有限公司 | It is a kind of to reduce the preparation method of blood fat chrysanthemum beverage |
| CN107535621A (en) * | 2017-07-19 | 2018-01-05 | 华南理工大学 | A kind of orange oil oil-bound distemper based on soluble soybean polysaccharide and preparation method thereof |
| CN108323569A (en) * | 2018-03-13 | 2018-07-27 | 重庆拜月食品科技有限公司 | Pasteurize goat milk and its manufacture craft |
| CN108477304A (en) * | 2018-03-13 | 2018-09-04 | 重庆拜月食品科技有限公司 | Liquid milk stabilizer and its application in liquid milk pasteurize |
| CN109354699A (en) * | 2018-08-01 | 2019-02-19 | 华南理工大学 | A kind of emulsion oil-in-water and preparation method thereof based on esterification soybean polyoses |
| CN111303308A (en) * | 2019-12-16 | 2020-06-19 | 福建省泉州市味博食品有限公司 | Production process of soluble soybean polysaccharide for rice and flour products |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107232353A (en) * | 2017-05-31 | 2017-10-10 | 北京佑众全椒制药有限公司 | A kind of preparation method of clearing heat and detoxicating chrysanthemum beverage |
| CN107296125A (en) * | 2017-05-31 | 2017-10-27 | 北京佑众全椒制药有限公司 | It is a kind of to reduce the preparation method of blood fat chrysanthemum beverage |
| CN107535621A (en) * | 2017-07-19 | 2018-01-05 | 华南理工大学 | A kind of orange oil oil-bound distemper based on soluble soybean polysaccharide and preparation method thereof |
| CN107535621B (en) * | 2017-07-19 | 2020-09-22 | 华南理工大学 | Soluble soybean polysaccharide-based orange oil powder and preparation method thereof |
| CN108323569A (en) * | 2018-03-13 | 2018-07-27 | 重庆拜月食品科技有限公司 | Pasteurize goat milk and its manufacture craft |
| CN108477304A (en) * | 2018-03-13 | 2018-09-04 | 重庆拜月食品科技有限公司 | Liquid milk stabilizer and its application in liquid milk pasteurize |
| CN109354699A (en) * | 2018-08-01 | 2019-02-19 | 华南理工大学 | A kind of emulsion oil-in-water and preparation method thereof based on esterification soybean polyoses |
| CN111303308A (en) * | 2019-12-16 | 2020-06-19 | 福建省泉州市味博食品有限公司 | Production process of soluble soybean polysaccharide for rice and flour products |
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