CN106267383A - 一种用于恢复剖腹产新生儿共生微生物抹布的制备方法 - Google Patents

一种用于恢复剖腹产新生儿共生微生物抹布的制备方法 Download PDF

Info

Publication number
CN106267383A
CN106267383A CN201610839501.8A CN201610839501A CN106267383A CN 106267383 A CN106267383 A CN 106267383A CN 201610839501 A CN201610839501 A CN 201610839501A CN 106267383 A CN106267383 A CN 106267383A
Authority
CN
China
Prior art keywords
neonate
cesarean
microorganism
vaginal secretions
gauze
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610839501.8A
Other languages
English (en)
Inventor
陈廷涛
辛洪波
杨少国
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanchang University
Original Assignee
Nanchang University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanchang University filed Critical Nanchang University
Priority to CN201610839501.8A priority Critical patent/CN106267383A/zh
Publication of CN106267383A publication Critical patent/CN106267383A/zh
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/005—Ingredients of undetermined constitution or reaction products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L31/16—Biologically active materials, e.g. therapeutic substances
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/426—Immunomodulating agents, i.e. cytokines, interleukins, interferons

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • Public Health (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Vascular Medicine (AREA)
  • Surgery (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

一种用于恢复剖腹产新生儿共生微生物抹布的制备方法，是将待产孕妇阴道分泌物用干净脱脂棉吸收，之后擦拭于剖腹产新生儿的嘴部、面部及身体的其他部位，弥补剖腹产新生儿细菌匮乏导致的后期免疫缺陷类疾病和抵抗力低下。本发明直接将待产孕妇的阴道分泌物取出，用于擦拭剖腹产新生儿嘴部、面部、肛门等身体的其他部位，恢复其共生微生物，具有简便、安全、有效、可操作性等优点。

Description

一种用于恢复剖腹产新生儿共生微生物抹布的制备方法
技术领域
本发明涉及一种用于恢复剖腹产新生儿共生微生物抹布的制备方法，属于微生物生物技术与生物医药领域。
背景技术
现代医学技术的发展为人类创造了剖腹产手术, 这种新的分娩方式挽救了无数产妇和婴儿的生命, 为保障人类的健康和人口再生长做出了巨大贡献。在阴道分娩无法达成, 或经阴道分娩可能对产妇或新生儿造成威胁时, 才需要剖腹产。然而近年来，我国剖腹产的孕妇数量逐年增多，如今我国的剖宫产率已经高达40%-50%，某些地区甚至更高。
分娩方式的选择直接影响新生儿共生菌群的组成。在顺产的婴儿中，观察到微生物组成往往与母体产道和肠道相似，而在剖腹产的婴儿中，微生物组成往往更接近于母亲的皮肤以及医院环境包括医护人员和其它新生儿。阴道分娩儿的菌群定植主要受母亲阴道和会阴区域菌群的影响，剖腹产儿则主要受医院环境的影响。以肠道菌群为例，前者最初的肠道菌群主要是母亲阴道和外阴周围的细菌，多为乳酸杆菌、普雷沃氏菌、双歧杆菌等；而后者主要以链球菌、葡萄球菌、厌氧球菌、类杆菌、丙酸杆菌为主。其中金黄色葡萄球菌是导致新生儿败血症的主要原因，双歧杆菌数量则很低甚至没有，而双岐杆菌对机体健康及免疫功能有重要作用。
正常阴道分娩过程中婴儿经过产道时会被产道挤压，在分娩过程中及出生后不久，母亲身体里的细菌就会通过胎儿呼吸道及消化道最早进入胎儿体内定植，这个细菌定植对婴儿一生的健康非常重要，它为一个基本无菌的新生儿肠道内建立起新的微生物生态系统。这些定植的细菌主要是在分娩过程，产道环境微生物起作用的结果。而新生儿的这一微生物生态系统的构成成分直接影响婴儿的免疫功能。剖腹产新生儿因出生时没有经产道挤压、不能及时建立共生菌群。最终造成剖腹产儿的微生物生态系统的构成与顺产儿有很大的不同。
大量的研究发现，剖腹产新生儿中观察到的微生物组成改变与远期多种疾病发生风险升高有着密切的关系，包括过敏、湿疹、哮喘、肥胖、1型糖尿病、慢性相关炎症性疾病等。英国研究人员警告说，剖腹产的孩子比顺产的孩子更易患哮喘，剖腹产孩子患哮喘的概率比正常顺产的孩子高80%。剖腹产剥夺了孩子最先感觉统合锻炼的权利，使得剖腹产儿容易患感统失调。而且剖腹产令“湿肺症”增多。总的来说，与自然分娩相比，剖腹产新生儿的免疫力及抗感染能力比自然分娩的孩子要差。
发明内容
本发明的目的是针对现有技术不足提供一种用于恢复剖腹产新生儿共生微生物抹布的制备方法，该方法具有简单方便、安全有效、可操作等优点，有助于更好地增强和提高剖腹产新生儿的抗感染能力和免疫功能。
本发明所述方法包括下列步骤：
1、阴道分泌物的收集和保存：在进行剖腹产手术之前1小时，将一块大小适宜的无菌纱布放置于待产母亲的阴道当中，手术开始时取出并保存在无菌容器中。
2、阴道分泌物的擦拭：在新生儿产下的1—3分钟内，将浸有阴道分泌物的纱布依次擦拭新生儿的嘴部、面部、肛门等身体的其他部位。
3、纱布微生物的鉴定：为验证纱布所浸的微生物与阴道分泌物的差异性，鉴定纱布以及母亲阴道分泌物样本中的菌群情况。
4、新生儿共生微生物的鉴定：为验证通过这种阴道菌群移植的方法是否可以恢复新生儿共生微生物，在新生儿出生的1、3、7、14、21及30天这六个时间点，分别在受试母婴的肛门、口腔及皮肤等部位共采集了样本，采用16S rRNA测序的方法鉴定其群落组成。
本发明的有益效果：本发明直接将待产孕妇的阴道分泌物取出，用于擦拭剖腹产新生儿嘴部、面部、肛门等身体的其他部位，恢复其共生微生物，该方法具有简单方便、安全有效、可操作等优点，有助于更好地增强和提高剖腹产新生儿的抗感染能力和免疫功能。
具体实施方式
本发明所述方法包括下列步骤：
1、阴道分泌物的收集和保存：在进行剖腹产手术之前1小时，将一块大小适宜的无菌纱布放置于待产母亲的阴道当中，手术开始时取出并保存在无菌容器中。
2、阴道分泌物的擦拭：在新生儿产下的1—3分钟内，将浸有阴道分泌物的纱布依次擦拭新生儿的嘴部、面部、肛门等身体的其他部位。
3、纱布微生物的鉴定：为验证纱布所浸的微生物与阴道分泌物的差异性，鉴定纱布以及母亲阴道分泌物样本中的菌群情况。
4、新生儿共生微生物的鉴定：为验证通过这种阴道菌群移植的方法是否可以恢复新生儿共生微生物，在新生儿出生的1、3、7、14、21及30天这六个时间点，分别在受试母婴的肛门、口腔及皮肤等部位共采集了样本，采用16S rRNA测序的方法鉴定其群落组成。

Claims (1)

1.一种用于恢复剖腹产新生儿共生微生物抹布的制备方法，其特征在于：所述方法包括下列步骤：
A、阴道分泌物的收集和保存：在进行剖腹产手术之前1小时，将一块大小适宜的无菌纱布放置于待产母亲的阴道当中，手术开始时取出并保存在无菌容器中;
B、阴道分泌物的擦拭：在新生儿产下的1—3分钟内，将浸有阴道分泌物的纱布依次擦拭新生儿的嘴部、面部、肛门身体的其他部位;
C、纱布微生物的鉴定：为验证纱布所浸的微生物与阴道分泌物的差异性，鉴定纱布以及母亲阴道分泌物样本中的菌群情况;
D、新生儿共生微生物的鉴定：为验证通过这种阴道菌群移植的方法是否可以恢复新生儿共生微生物，在新生儿出生的1、3、7、14、21及30天这六个时间点，分别在受试母婴的肛门、口腔及皮肤部位共采集了样本，采用16S rRNA测序的方法鉴定其群落组成。
CN201610839501.8A 2016-09-22 2016-09-22 一种用于恢复剖腹产新生儿共生微生物抹布的制备方法 Pending CN106267383A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610839501.8A CN106267383A (zh) 2016-09-22 2016-09-22 一种用于恢复剖腹产新生儿共生微生物抹布的制备方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610839501.8A CN106267383A (zh) 2016-09-22 2016-09-22 一种用于恢复剖腹产新生儿共生微生物抹布的制备方法

Publications (1)

Publication Number Publication Date
CN106267383A true CN106267383A (zh) 2017-01-04

Family

ID=57713218

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610839501.8A Pending CN106267383A (zh) 2016-09-22 2016-09-22 一种用于恢复剖腹产新生儿共生微生物抹布的制备方法

Country Status (1)

Country Link
CN (1) CN106267383A (zh)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110141768A (zh) * 2019-06-11 2019-08-20 河南省人民医院 一种孕妇阴道微生物新生儿体表转移装置
CN111445957A (zh) * 2020-02-25 2020-07-24 北京德润良品健康科技有限公司 一种母婴菌群传递监测系统及方法
WO2021170018A1 (zh) * 2020-02-25 2021-09-02 陈择宁 一种用于母婴菌群采集及传递的待产包

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102919663A (zh) * 2005-10-21 2013-02-13 荷兰纽迪希亚公司 预防剖腹产分娩的婴儿疾病的方法
CN105288605A (zh) * 2015-11-13 2016-02-03 刘传新 剖宫产婴儿首次细菌接种方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102919663A (zh) * 2005-10-21 2013-02-13 荷兰纽迪希亚公司 预防剖腹产分娩的婴儿疾病的方法
CN105288605A (zh) * 2015-11-13 2016-02-03 刘传新 剖宫产婴儿首次细菌接种方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
郭贺: ""不同分娩方式婴儿肠道菌群对比分析"", 《中国博士学位论文全文数据库 医药卫生科技辑（月刊）》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110141768A (zh) * 2019-06-11 2019-08-20 河南省人民医院 一种孕妇阴道微生物新生儿体表转移装置
CN111445957A (zh) * 2020-02-25 2020-07-24 北京德润良品健康科技有限公司 一种母婴菌群传递监测系统及方法
WO2021170018A1 (zh) * 2020-02-25 2021-09-02 陈择宁 一种用于母婴菌群采集及传递的待产包
CN111445957B (zh) * 2020-02-25 2024-05-24 北京德润良品健康科技有限公司 一种母婴菌群传递监测系统及方法

Similar Documents

Publication Publication Date Title
Williams Healthy carriage of Staphylococcus aureus: its prevalence and importance
Mändar et al. Transmission of mother’s microflora to the newborn at birth
CN106267383A (zh) 一种用于恢复剖腹产新生儿共生微生物抹布的制备方法
McIntyre An epidemic of Streptococcus pyogenes puerperal and postoperative sepsis with an unusual carrier site—the anus
CN107050063A (zh) 治疗过敏体质的乳酸菌组合物及其制备方法
CN105288605A (zh) 剖宫产婴儿首次细菌接种方法
Drusin et al. Group C streptococcal colonization in a newborn nursery
Boissard et al. Neonatal umbilicus as a source of streptococcal infections in a maternity unit
CN106635868A (zh) 一种增强婴幼儿免疫力的人工微生态制剂的制备方法
MAcKNIGHT et al. Group B streptococci in neonatal deaths
Bygdeman et al. Hemolytic streptococci among infants in a maternity department: report of an outbreak
Anthony Epidemiology of GBS in man
Pisaniak et al. Corrective factors of intestinal microflora disorders in the perinatal period
CN205170847U (zh) 剖宫产婴儿首次细菌接种器
CN104397077A (zh) 新型灭菌液
Chand et al. Intra-natal care practices of staff nurses with compliance to professional protocols
Gilliatt Placenta praevia in four successive pregnancies
Cosgrove et al. Antepartum Gas-Bacillus Infection: Report of a Case with Septicemia and Recovery but with Death of the Fetus
CN105255714A (zh) 剖宫产婴儿首次细菌接种器
GO et al. Comparative efficacy of oral Lactobacillus rhamnosus (protexin) against metronidazole (flagyl) in the treatment of bacterial vaginosis: A randomized clinical trial.
UA117914U (uk) Спосіб профілактики інфекційно-запального ураження плода при передчасному розриві плодових оболонок
Burak et al. Investigation of the Relationship Between Neuromotor Behavior and Pain, Comfort, Apgar Score and Clinical Factors in Premature Neonates
Özek et al. Vaginal birth after cesarean (vbac) success and complication ratios in our clinic
KOTWANI PULMONARY EMBOLISM ASSOCIATED WITH HYDATIDIFORM MOLE
Briggs et al. Pneumonia found at autopsy in infants weighing less than 750 grams

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20170104

WD01 Invention patent application deemed withdrawn after publication