CN106267375B - A kind of 3 D-printing bracket of mesoporous bioglass containing strontium and preparation method thereof carrying isoflavones - Google Patents
A kind of 3 D-printing bracket of mesoporous bioglass containing strontium and preparation method thereof carrying isoflavones Download PDFInfo
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- CN106267375B CN106267375B CN201610791436.6A CN201610791436A CN106267375B CN 106267375 B CN106267375 B CN 106267375B CN 201610791436 A CN201610791436 A CN 201610791436A CN 106267375 B CN106267375 B CN 106267375B
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/10—Ceramics or glasses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
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- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y70/00—Materials specially adapted for additive manufacturing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
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- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
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- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
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Abstract
The invention discloses a kind of 3 D-printing brackets of mesoporous bioglass containing strontium for carrying isoflavones, belong to field of biomedical materials.The bracket is made of mesoporous bioglass containing strontium, polycaprolactone and isoflavones, wherein the mass ratio of Sr-MBG and PCL is respectively 70% and 30%, 5% Ca is substituted by Sr in Sr-MBG, by isoflavones be loaded into Sr-MBG it is mesoporous in, using three-dimensional printing technology, the two kinds of materials of Sr-MBG and PCL for being loaded with isoflavones are successively printed to the bioactive bracket as cube, each layer is made of criss-cross vertical line, and the hole that vertical line is constituted is square.Bracket of the invention can be applied to the reparation and treatment for bone defect, with good biocompatibility, there is the preferable ability for promoting skeletonization and can be absorbed by living organism and degraded, and therapeutic effect to sufferers of osteoporosis face bone defect can be enhanced after being loaded into isoflavones, it is expected to become the comparatively ideal timbering material of bone tissue engineer.
Description
Technical field
The invention belongs to field of biomedical materials, and in particular to a kind of 3 D-printing for carrying isoflavones is mesoporous containing strontium
Bio-vitric bracket.
Background technique
According to incompletely statistics, the whole world is every year because the bone defect caused by a variety of causes need to use the patient of bone renovating material
Ten thousand more than 700, currently used bone renovating material includes autologous bone, allograph bone, hydroxyapatite, bioactive ceramics, life
Object glass, inert material etc..There is supply deficiency as bone renovating material, infection, may spread the disease in autologous bone, allograph bone
Disadvantage, and hydroxyapatite and inert material presence do not have the disadvantages of bioactivity and non-degradable, so next life in recent years
The degradable bone implant material of object activity becomes the research hotspot of bone renovating material.
The method that tradition prepares bracket is difficult to control aperture size, the connection in hole and the porosity of entire bracket.And it adopts
It can be very good the porosity of the control form in aperture, size and bracket with newest three-dimensional printing technology.This technology it is excellent
Gesture is that we can accurately control the structure of institute's print carriage with the help of computer aided design system (CAD), and
The bracket of printing has good mechanical strength.
Chinese patent application CN201510537311.6 discloses a kind of bone based on 3D-Bioplotter printing technique
Porous compound support frame is repaired,.The bracket uses 3D- by having the matrix of three-dimensional macroporous structure and drug bearing microsphere to be combined first
Bioplotter prints the rest body with the three-dimensional macroporous structure of rule；Emulsion-solvent evaporation method prepares compound hexagonal mesoporous
The drug bearing microsphere of silicon (HMS), calcium silicate powder (CS) and PLGA；Complex microsphere is fixed to matrix material finally by low-temperature sintering
In material, compound rest is made.The compound rest has good load medicine Release Performance.
Chinese patent application CN201510674031.X discloses a kind of life that 3D printing mesopore bioactive glass is modified
Object ceramics bracket, the bracket are the bata-tricalcium phosphate bioactive bracket and uniformly coating using three-dimensional printing technology preparation
In the mesopore bioactive glass of the rack surface；, in the mesopore bioactive glass presoma (specially containing the source Si
Ethyl orthosilicate), triethyl phosphate, calcium nitrate etc..
Bioactivity glass abbreviation bio-vitric is a kind of artificial synthesized bone tissue alternative materials, the Ca contained2+With
PO in biological fluid4 3-To SiO2Gel layer diffusion deposits, gradually mineralising, and then is formed on its surface hydroxyapatite covering
Layer, realizes the combination of itself and bone tissue.Bio-vitric has good osteoinductive, osteoconductive and biocompatibility.By
The research in more than 20 years is used for Orthopedic Clinical through U.S.'s food and Drug Administration's official approval in the nineties, has been found
There is good Bone Defect Repari function.Strontium element (Strontium, Sr), which is found to have, in recent years promotes skeletonization and inhibition osteoclast
Effect, and it is clinically used for treatment osteoporosis.Since strontium and calcium belong to same period element, similar in the chemically, institute
Calcium can be replaced with strontium, and be used for modified different kind organism activity bone implant material (such as bioceramic, bio-vitric or polymer
Deng).Strontium ion state is formed as material degradation strontium element can be released by the modified bio-vitric of strontium, thus
It plays and promotes skeletonization or inhibit osteoclastic effect, so strontium modified degradable bioactivity bone implant material theoretically has its section
Learn reasonability.
Isoflavones is a type phytoestrogen, the parent nucleus knot of mother nucleus structure (isoflavones structure) and estrogen
Structure, thus there is molecular structure similar with estrogen, there is certain weak estrogenic activity, by competing with estrogen receptor
Striving property combines, and effectively bidirectional modulation human body inner estrogen is horizontal, so that playing quasi-waverider vehicle (improves internal estrogen water
Flat, play estrogenic activity enhancement effect) and antiestrogenic (reduce internal estrogen level, play estrogenic activity and press down
Effect processed).It is improved currently with the quasi-waverider vehicle of more mainly isoflavones by supplementing isoflavones
The intracorporal estrogen level of middle and aged women plays the role of preventing and treating osteoporosis.
There is no literature reported on the 3 D-printing brackets of mesoporous bioglass containing strontium in relation to carrying isoflavones at present.
Summary of the invention
In order to solve the deficiencies in the prior art, it is mesoporous containing strontium that the present invention provides a kind of 3 D-printings for carrying isoflavones
The 3 D-printing bracket of mesoporous bioglass containing strontium of bio-vitric bracket and preparation method thereof, the load isoflavones can be applied to
For the reparation and treatment of bone defect, with good biocompatibility, there is the preferable ability for promoting skeletonization and can be given birth to
Object body absorbs degradation, and can enhance the therapeutic effect to sufferers of osteoporosis face bone defect after loading isoflavones, is expected into
For the comparatively ideal timbering material of bone tissue engineer.
The present invention devises a kind of 3 D-printing bracket of mesoporous bioglass containing strontium for carrying isoflavones, is beaten using three-dimensional
Two kinds of materials of Sr-MBG and PCL are successively printed the bioactive bracket as cube, then isoflavones are born by print technology
Be downloaded to bio-vitric it is mesoporous in, make it with the absorption slow release of bracket, treat osteoporosis.
The first aspect of the present invention provides a kind of 3 D-printing branch of mesoporous bioglass containing strontium for carrying isoflavones
Frame, the bracket are formed by containing strontium (Sr) mesoporous bioglass (Sr-MBG), polycaprolactone (PCL) and isoflavones, wherein
The mass ratio of Sr-MBG and PCL is respectively 70% and 30%, and 5% Ca is substituted by Sr in Sr-MBG, and isoflavones is carried
Enter Sr-MBG it is mesoporous in, play the role of treat osteoporosis；The bracket uses three-dimensional printing technology, will have soybean
Two kinds of materials of Sr-MBG and PCL of isoflavones successively print the bioactive bracket as cube, and each layer is by criss-cross
Vertical line is constituted.
The bracket is cube, and the line of same layer is parallel lines and spacing is equal, and adjacent two layers are hung down by criss-cross
Straight line is constituted, i.e. all warps of first layer, all wefts of the second layer, third layer and all warps, and the 4th layer again whole
The hole constituted for the criss-cross vertical line of weft ... is square.
The aperture is preferably 400 μm of side length of square, and is connected to up and down.Be conducive to osteocyte and new vessels
It grows into.
The bracket can be applied to the reparation and treatment for bone defect, has good biocompatibility, has preferably
Promote the ability of skeletonization and can be absorbed by living organism to degrade；It can enhance after the bracket loading isoflavones and sclerotin is dredged
The therapeutic effect of loose patient's bone defect.
Preferably, Sr-MBG powder is by 57.2SiO used in bracket2:7.5P2O5: 35.3 (SrO+CaO) (molar ratios
Example) it constitutes, wherein 5% Ca is replaced by Sr, the powder being finally synthesizing is 5Sr-MBG, and isoflavones is loaded into Sr-MBG's
In mesoporous.
Preferably, the mass ratio of Sr-MBG powder and PCL are respectively 70% and 30%, PCL is dissolved in chloroform,
Then load isoflavones Sr-MBG powder is added to be uniformly dissolved.
The second aspect of the present invention provides a kind of 3 D-printing bracket of mesoporous bioglass containing strontium for carrying isoflavones
Preparation method, the described method comprises the following steps:
Step 1 prepares bioactive glass powder containing strontium, and crosses the sieve of 400 mesh, makes its size less than 37 μm, will be big
Beans isoflavones dissolution after be loaded into Sr-MBG it is mesoporous in, powder drying saves backup；
The preparation method of the bioactive glass powder containing strontium, referring to document: Zhang J, Zhao S, Zhu Y, et
al.Three-dimensional printing of strontium-containing mesoporous bioactive
glass scaffolds for bone regeneration[J].Acta Biomaterialia,2014,10(5):2269-
2281. preferably wherein 5% Sr-MBG.
Polycaprolactone (PCL) is dissolved in chloroform, magnetic agitation 2 hours, is sealed spare by step 2；
The bioactive glass powder containing strontium for the load isoflavones that step 1 obtains is added to PCL solution by step 3
In, mass ratio 7:3 is quickly stirred evenly and is made into marking ink, is sealed spare.
Step 4 is put into the marking ink of step 3 in the barrel of three-dimensional printer, and needle diameter is 250 microns；It opens
Dynamic 3 D-printing program, adjustings air pressure are 1.5-3bar, print speed 1.5-5mm/s, and ink adjacent two layers move towards angle and are
0 ° and 90 °, pitch of holes is 400 microns, ink is deposited in a manner of layer upon layer in the glass culture dish of article carrying platform, 37
DEG C dry 48 hours, obtain the 3 D-printing bracket of mesoporous bioglass containing strontium for carrying isoflavones.
Preferably, in step 1, isoflavones is dissolved in obtained in PBS solution 100mg/ml isoflavones it is molten
The powder of mesoporous bioglass containing strontium is soaked in the PBS solution containing 100mg/ml 24 hours, isoflavones is loaded by liquid
Sr-MBG it is mesoporous in.
Preferably, 3 D-printing is using forth generation 3D BioplotterTM(EnvisionTEC GmbH,
Germany), jet diameters 0.25mm, jet velocity 1.5-5mm/s, the pressure of syringe pump are 1.8bar, are successively printed.
Preferably, bracket is made of criss-cross vertical line, the distance between line and line are 400 μm, bracket aperture
Diameter be 400 μm, be conducive to growing into for osteocyte and new vessels, stent size 10mm*10mm*3mm.
Preferably, reaching it with bracket absorption in the mesoporous interior loading isoflavones of the bracket of bio-vitric containing strontium
To the effect of sustained release, osteoporosis is treated.
The third aspect of the present invention provides a kind of 3 D-printing bracket of mesoporous bioglass containing strontium for carrying isoflavones
Preparing the application in bone tissue engineer repair materials.
The beneficial effects of the present invention are: a kind of 3 D-printing mesoporous biological containing strontium for carrying isoflavones provided by the invention
Glass supporter can be applied to reparation and treatment for bone defect, and can carry out individuation according to the form of patient's bone defect
3 D-printing bone filling bracket, achievees the purpose that individualized treatment；And this bracket has good biocompatibility, has preferably
Promotion skeletonization ability and can by living organism absorb degrade；Bracket aperture is designed as the square hole that diameter is 400 μm,
Be conducive to growing into for osteocyte and new vessels；By isoflavones load to bio-vitric it is mesoporous in, make it with bracket
Absorption slow release, play the role for the treatment of osteoporosis, interior animal experiment further demonstrates bracket of the invention
With excellent internal osteogenic activity, it is expected to become the comparatively ideal timbering material of bone tissue engineer.
The 3 D-printing bracket of mesoporous bioglass containing strontium of load isoflavones of the invention can be applied to for bone defect
Reparation and treatment, with good biocompatibility, have the preferable ability for promoting skeletonization and can be absorbed by living organism
Degradation, and osteoporosis of normal middle-omd age women can be treated, it is expected to become the comparatively ideal timbering material of bone tissue engineer.
Detailed description of the invention
Fig. 1 is a kind of pictorial diagram of the 3 D-printing bracket of mesoporous bioglass containing strontium of load isoflavones of the invention；
Fig. 2 is a kind of schematic diagram of the 3 D-printing bracket of mesoporous bioglass containing strontium of load isoflavones of the invention；
Fig. 3 is a kind of scanning electron microscope of the 3 D-printing bracket of mesoporous bioglass containing strontium of load isoflavones of the invention
Figure；
Fig. 4 cell cultivates 7 days SEM figures on the 3 D-printing bracket of mesoporous bioglass containing strontium for carrying isoflavones.
Specific embodiment
Present invention will be further explained below with reference to specific examples, for explaining only the invention, and should not be understood as to this
The limitation of invention.
In the following examples, the experimental methods for specific conditions are not specified, usually according to normal condition or according to proposed by manufacturer
Condition implement.
The reagent that source is not specified in the following example can be obtained from commercial routes.
A kind of embodiment 1: preparation for the 3 D-printing bracket of mesoporous bioglass containing strontium carrying isoflavones
A kind of preparation method of 3 D-printing bracket of mesoporous bioglass containing strontium carrying isoflavones of the present invention, packet
Include following steps:
Step 1 prepares bioactive glass powder containing strontium, and crosses the sieve of 400 mesh, makes its size less than 37 μm, will be big
Beans isoflavones is dissolved in phosphate buffered saline solution (phosphate buffer saline, PBS) and obtains the soybean of 100mg/ml
The powder of mesoporous bioglass containing strontium is soaked in the PBS solution containing 100mg/ml 24 hours by isoflavones solution, and soybean is different
Flavones be loaded into Sr-MBG it is mesoporous in；Powder drying saves backup；
The preparation method of the bioactive glass powder containing strontium is referring to document Zhang J, Zhao S, Zhu Y, et
al.Three-dimensional printing of strontium-containing mesoporous bioactive
glass scaffolds for bone regeneration[J].Acta Biomaterialia,2014,10(5):2269-
2281. preferably wherein 5% Sr-MBG.
Polycaprolactone (PCL, molecular weight are that 70000-90000 is purchased from Sigma-Aldrich company) is dissolved in chlorine by step 2
It is imitative, it magnetic agitation 2 hours, is sealed spare；
The bioactive glass powder containing strontium for the load isoflavones that step 1 obtains is added to PCL solution by step 3
In, mass ratio 7:3 is quickly stirred evenly and is made into marking ink, is sealed spare.
Step 4 is put into the marking ink of step 3 in the barrel of three-dimensional printer, and needle diameter is 250 microns；It opens
Dynamic 3 D-printing program, adjusting air pressure are 1.8bar, and print speed 1.5-5mm/s, it is 0 ° that ink adjacent two layers, which move towards angle,
With 90 °, pitch of holes be 400 microns, ink is deposited in a manner of layer upon layer in the glass culture dish of article carrying platform, 37 DEG C
It is 48 hours dry, obtain the 3 D-printing bracket of mesoporous bioglass containing strontium for carrying isoflavones.
A kind of pictorial diagram such as Fig. 1 institute of the 3 D-printing bracket of mesoporous bioglass containing strontium of load isoflavones of the invention
Show；Structural schematic diagram is as shown in Figure 2；Scanning electron microscope (SEM) photograph is as shown in figure 3, illustrate the 3 D-printing of load isoflavones of the invention
The bracket adjacent two layers of mesoporous bioglass containing strontium are criss-cross latitude and longitude, and criss-cross latitude and longitude constitute equally distributed
Intercommunicating pore, hole are square.Bracket of the invention is well combined, no division and collapse phenomenon.
Embodiment 2:MC3T3-E1 cell is on the 3 D-printing bracket of mesoporous bioglass containing strontium for carrying isoflavones
Adherency
MC3T3-E1 cell (mice embryonic pre-osteoblast) is purchased from Chinese Academy of Sciences's cell bank.
The 3 D-printing timbering material sample of mesoporous bioglass containing strontium all uses medicinal alcohol immersion 4 hours of 75%, so
It irradiates and is sterilized for 24 hours in the UV lamp afterwards, meanwhile, α-MEM culture medium of the lml containing 10%FBS is added in 24 orifice plates and soaks in advance
For 24 hours, culture medium is replaced, later moves into sterilizing timbering material in 24 orifice plates.Then, 1 × 10 will be contained5MC3T3-E1 cell
The culture solution of 200 μ 1 is added drop-wise to each sample, and in 37 DEG C, 5%CO2The CO of atmosphere2It is cultivated in incubator, to 8 hour cell bases
After this is adherent, α-MEM culture medium of the 1ml containing 10%FBS is added.
After cell cultivates 3 days and 7 days on bracket, first washed three times at 37 DEG C with PBS buffer solution, later all samples
1h is fixed with the PBS containing 2.5% glutaraldehyde, fixative is removed by the buffer solution PBS containing 4% sucrose, passed through later
PBS buffer solution containing 1% osmium tetroxide is fixed, then by graded ethanol solutions (50%, 70%, 90%,
95%, 100%) and hexamethyldisilazane solution (HMDS) dehydration.Last SEM (FEI Quanta 450) observation cell is propping up
The adherency situation on frame surface, all bracket samples are adhesive on metal base with conduction, carry out metal spraying processing before observing.By sweeping
Cell morphology and adherency after retouching Electronic Speculum (SEM) and cultivating 3 days and 7 days observing cell on bracket.
Fig. 4 is the SEM picture that MC3T3-E1 cell and bracket co-culture 7 days after-poppet surfaces, it is possible thereby to observe cell
Adherency and pattern in rack surface.As seen from the figure, MC3T3-E1 cell on bracket can full extension, display is good
Cell morphology.
The preferred embodiment of the present invention has been described in detail above, but the invention be not limited to it is described
Embodiment, those skilled in the art can also make various equivalent on the premise of not violating the inventive spirit of the present invention
Variation or replacement, these equivalent variation or replacement are all included in the scope defined by the claims of the present application.
Claims (7)
1. a kind of 3 D-printing bracket of mesoporous bioglass containing strontium for carrying isoflavones, which is characterized in that the bracket is
Successively being printed timbering material using three-dimensional printing technology becomes cube, and the line of same layer is parallel lines and spacing is equal, adjacent
It is made of criss-cross vertical line for two layers, the hole that criss-cross vertical line is constituted is square；
The timbering material is made of mesoporous bioglass containing strontium, polycaprolactone and isoflavones, and isoflavones carries
Enter the mesoporous bioglass containing strontium it is mesoporous in, wherein the mass ratio of mesoporous bioglass containing strontium and polycaprolactone be 70%:30%；
5% Ca is substituted by Sr in the mesoporous bioglass containing strontium；
The preparation method of the 3 D-printing bracket of mesoporous bioglass containing strontium of the described load isoflavones the following steps are included:
Step 1 prepares bioactive glass powder containing strontium, and crosses the sieve of 400 mesh, makes its size less than 37 μm, and soybean is different
Flavones dissolution after be loaded into the bioactive glass powder containing strontium it is mesoporous in, powder drying saves backup；
Polycaprolactone is dissolved in chloroform by step 2, and magnetic agitation 1-3 hours；
The bioactive glass powder containing strontium for the load isoflavones that step 1 obtains is added to polycaprolactone solution by step 3
In, mass ratio 7:3 is stirred evenly and is made into marking ink；
Step 4 is put into the marking ink of step 3 in the barrel of three-dimensional printer, jet diameters 0.25mm, jet velocity
For 1.5-5mm/s, the pressure of syringe pump is 1.5-3bar, is successively printed.
2. the 3 D-printing bracket of mesoporous bioglass containing strontium according to claim 1 for carrying isoflavones, feature exist
In the composition of the mesoporous bioglass containing strontium is as follows: SiO2:P2O5: the molar ratio of (SrO+CaO) is 57.2:7.5:35.3, wherein
The molar ratio that Sr accounts for (SrO+CaO) is 5%.
3. the 3 D-printing bracket of mesoporous bioglass containing strontium according to claim 1 or 2 for carrying isoflavones, feature
It is, the distance between the support wire and line are 400 μm, the square that aperture is 400 μm of side length, and cube size is
10mm*10mm*3mm。
4. the preparation method of the 3 D-printing bracket of mesoporous bioglass containing strontium of isoflavones is carried as described in claim 1,
It is characterized in that, the preparation method the following steps are included:
Step 1 prepares bioactive glass powder containing strontium, and crosses the sieve of 400 mesh, makes its size less than 37 μm, and soybean is different
Flavones dissolution after be loaded into the bioactive glass powder containing strontium it is mesoporous in, powder drying saves backup；
Polycaprolactone is dissolved in chloroform by step 2, and magnetic agitation 1-3 hours；
The bioactive glass powder containing strontium for the load isoflavones that step 1 obtains is added to polycaprolactone solution by step 3
In, mass ratio 7:3 is stirred evenly and is made into marking ink；
Step 4 is put into the marking ink of step 3 in the barrel of three-dimensional printer, jet diameters 0.25mm, jet velocity
For 1.5-5mm/s, the pressure of syringe pump is 1.5-3bar, is successively printed.
5. the preparation side of the 3 D-printing bracket of mesoporous bioglass containing strontium according to claim 4 for carrying isoflavones
Method, which is characterized in that in step 1, the bracket of mesoporous bioglass containing strontium is soaked in the PBS solution containing 100mg/ml 24
Hour, then by isoflavones be loaded into bracket it is mesoporous in.
6. the preparation of the 3 D-printing bracket of mesoporous bioglass containing strontium according to claim 4 or 5 for carrying isoflavones
Method, which is characterized in that the 3 D-printing is using forth generation 3D BioplotterTM(EnvisionTEC GmbH,
Germany), adjusting air pressure is 1.8bar, and print speed 1.5-5mm/s, it is 0 ° and 90 ° that ink adjacent two layers, which move towards angle,
Pitch of holes is 400 microns, ink is deposited in a manner of layer upon layer in the glass culture dish of article carrying platform, 37 DEG C of dryings 48
Hour to get.
7. the 3 D-printing bracket of mesoporous bioglass containing strontium for carrying isoflavones as described in claim 1 is preparing bone tissue
Application in engineering repair materials.
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| CN111643725B (en) * | 2020-06-01 | 2021-05-07 | 四川大学 | Artificial bone material for repairing bone defect and preparation method of artificial bone particles |
| CN114259604B (en) * | 2021-12-17 | 2022-12-27 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method of 3D printing ordered vascularization promoting drug-loaded bone repair scaffold, product and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104027849A (en) * | 2014-06-30 | 2014-09-10 | 四川大学 | Soy isoflavone supported biological composite material porous scaffold and preparation |
| CN105582571A (en) * | 2016-02-24 | 2016-05-18 | 浙江大学 | Manufacturing method of high-strength bioactive porous scaffold |
-
2016
- 2016-08-31 CN CN201610791436.6A patent/CN106267375B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104027849A (en) * | 2014-06-30 | 2014-09-10 | 四川大学 | Soy isoflavone supported biological composite material porous scaffold and preparation |
| CN105582571A (en) * | 2016-02-24 | 2016-05-18 | 浙江大学 | Manufacturing method of high-strength bioactive porous scaffold |
Non-Patent Citations (2)
| Title |
|---|
| In vitro and in vivo bone formation potential of surface calcium phosphate-coated polycaprolactone and polycaprolactone/bioactive glass composite scaffolds;Patrina S.P. Poh et al.;《Acta Biomaterialia》;20151110;第30卷;319–333页 * |
| Three-dimensional printing of strontium-containing mesoporous bioactive glass scaffolds for bone regeneration;Jianhua Zhang et al.;《Acta Biomaterialia》;20140110;第10卷;2269–2281页 * |
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