CN106267339A - A kind of high-modulus super hydrophilic biological support preparation method - Google Patents
A kind of high-modulus super hydrophilic biological support preparation method Download PDFInfo
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Abstract
The invention discloses a kind of high-modulus super hydrophilic biological support preparation method, the present invention prepares synthesis/natural polymer composite fibre biological support by electrostatic spinning technique, and modifies composite fiber surface character by the method for poly-dopamine face coat.Composite fibrous scaffold prepared by the method has sticking, breed and breaking up of high-modulus, Superhydrophilic and good biocompatibility, beneficially cell.By the introducing of natural polymer, make fibrous framework while having excellent mechanical performances, there is good hydrophilicity and biocompatibility, and be effectively improved the poly-dopamine coating result at composite fibrous scaffold.By dopamine in composite fiber surface auto polymerization, improve the hydrophilicity of support, mechanical property and biocompatibility further.Based on electrospinning device, simple, available material category is various, conveniently prepare multicomponent composite materials and its advantage such as the nanofiber specific surface area prepared is big, porosity is high, internal connectivity is good.
Description
Technical field
The invention belongs to field of high polymer material processing, particularly relate to a kind of high-modulus super hydrophilic biological support preparation side
Method.
Background technology:
The biological support place as repopulating cell and the template of tissue regeneration, cause increasingly in field of tissue engineering technology
Many concerns.But, the most prepared biological support majority cannot be provided simultaneously with good mechanical property, hydrophilicity and life
The thing compatibility.The most how to prepare and have the ideal biological support of good combination property and be still current challenging problem.
Be simple and convenient to operate based on electrospinning device, available material category is various, conveniently prepare multicomponent composite materials and
The pulp freeness obtained is big, homogeneity advantages of higher, and it efficiently prepares the technology of composite fibre biological support as ideal
Paid close attention to widely and applied.
2003, Yoshimoto, H. etc. were prepared for polycaprolactone (PCL) support by method of electrostatic spinning.First will be certain
The PCL of amount is dissolved in chloroform, and under room temperature, concussion obtains the uniform solution that concentration is 10% (w/v), under the spinning voltage of 13kV, poly-
Polymer solution is stretched filamentation by electrostatic field force, obtains non-woven fabrics PCL fiber.Then, support is immersed in the ethanol of 70% kills
Poison, and being soaked in collagen solution, to carry out face coat modified, finally uses mesenchymal stem cells to be planted on support.Knot
Fruit shows, the modified support of collagen coating has good biocompatibility.But the support that the method prepares exists
While improving biocompatibility, mechanical property is not improved.
2005, Vaz, C.M. etc. were prepared for double layer fibre support by method of electrostatic spinning.They room temperature by PCL and
PLA is dissolved in chloroform and chloroform/dimethylformamide (15:3) mixed solution respectively, it is thus achieved that concentration be 12.5% (w/v) and
The homogeneous polymer solution of 14.0% (w/v).First choosing PCL solution at voltage is 13kV, accepts distance for 20cm, solution
Advancing speed is to carry out electrostatic spinning under conditions of 10 μ L/min, uses cylinder collection method (rotating speed is 60Hz), after 180min
Obtain the PCL support of internal layer.Then being 13kV by PLA solution at voltage, accept distance for 20cm, solution flow rate is 25 μ L/
Min, drum rotation speed is to carry out electrostatic spinning under conditions of 180Hz, and PLA fiber is collected on PCL fiber, prepares after 60min
Obtain double-deck PLA/PCL fibrous framework.The PLA/PCL double-layer scaffold that the method prepares can be effectively improved its mechanics
Performance, but its hydrophilicity and biocompatibility cannot be improved.
2009, Zoppe, J.O. etc. were successfully prepared PCL/ nano-cellulose crystal grain composite fibre by method of electrostatic spinning
Support.First they use a series of chemical treatment method to extract nano-cellulose crystal grain, subsequently, use 2,4-toluene two
It is modified by isocyanates.Finally a certain amount of modified nano-cellulose crystal grain is scattered in diformazan by supersound process
Base formamide solution, weighs the concussion of 15g PCL room temperature simultaneously and is dissolved in dichloromethane solution, finally two kinds of solution mixing is obtained
Spinning liquid, wherein in spinning liquid, nano-cellulose crystal grain accounts for the mass ratio of PCL and is respectively 2.5% and 5%.Electric field be 0.5～
1.0kV/cm, advances speed 0.8～1.0mL/h, and accepting distance is to carry out electrostatic spinning under conditions of 10～20cm, prepares
PCL/ nano-cellulose crystal grain compound rest.Result shows, the support prepared has preferable mechanical property and hydrophilic
Energy.
2014, Jing, X. etc. were prepared for the PCL three-dimensional rack with shish-kebab structure by method of electrostatic spinning.First will
A certain amount of PCL is dissolved in the mixed solution of chloroform/dimethylformamide (6:4), and it is the equal of 12% (w/v) that room temperature concussion obtains concentration
One spinning liquid.Being 18kV at voltage, collection distance is 20cm, and advancing speed is to carry out electrostatic spinning under conditions of 0.5mL/h,
Obtain PCL fibrous framework.Then, a certain amount of PCL is dissolved in the mixed solution of deionized water/acetic acid (23:77), 60 DEG C of magnetic
Power stirring 1h, it is thus achieved that the PCL weak solution of 0.8% (w/v), takes the PCL weak solution of 30 μ L, slowly drops on PCL fibrous framework, room
Temperature deionized water rinsing twice, obtains the PCL support with shish-kebab structure after vacuum drying.Finally, use matrigel that PCL is propped up
Frame surface is modified further.Test result indicate that: on cell support after modification, there is good adhesion and propagation.
2014, Mandal, A. etc. were prepared for polyvinyl alcohol (PVA)/nano-cellulose composite cellulosic membrane.First by fiber
Element adds in 60% sulphuric acid of 50 DEG C, and stirring 5h makes it hydrolyze.Be washed with deionized subsequently, centrifugation is until solution ph
It is 7.And by suspension ultrasonic 5min in ice-water bath, prepare nano-cellulose suspension.Secondly, by molten for a certain amount of PVA
In deionized water, vibration obtains the homogeneous PVA solution of 5% (w/v), and is joined by a certain amount of nano-cellulose suspension
In PVA solution, after mechanical agitation 2h, ultrasonic 2min obtains PVA/ nano-cellulose mixed solution.Finally, mixed solution is poured into
In culture dish, drying at room temperature 2d, it is dried in the vacuum drying oven of 60 DEG C subsequently, obtains PVA/ nano cellulose composite film.Knot
Fruit shows: the addition of nano-cellulose effectively raises the mechanical property of composite cellulosic membrane.
2015, Mo, Y. etc. were prepared for Poly(D,L-lactide-co-glycolide (PLGA)/Nanowire by method of electrostatic spinning
Dimension element crystal grain composite fibrous scaffold.First, take the sulfuric acid solution that 10g cellulose joins the 65% of 95mL, stir 5h at 55 DEG C
Be hydrolyzed reaction, obtains suspension.Subsequently suspension is centrifuged 10min, and ultrasonic 15min, with deionized water clean until
Solution ph is 7, then within 1 week, removes unnecessary sulphuric acid by dialyzer dialysis treatment.Secondly, by nano-cellulose grain dispersion
In the PLGA solution (hexafluoroisopropanol is solvent) of 15% (w/v), obtain the mixing of uniform PLGA/ nano-cellulose crystal grain molten
Liquid.Finally, being 15kV at voltage, collection distance is 10cm, and advancing speed is to carry out Static Spinning under conditions of 1.0～2.0mL/h
Silk, prepares PLGA nano-cellulose crystal grain composite fibrous scaffold.Test result indicate that, the introducing of nano-cellulose is obvious
Improve mechanical property and the hydrophilicity of composite fibrous scaffold.
2010, Jiang, J.H. etc. successfully utilized poly-dopamine that polyethylene (PE) perforated membrane is carried out surface modification.First
First, take a certain amount of dopamine and be dissolved in Tris-HCl buffer (pH=8.5) and alcohol mixed solution (9:1), obtain concentration
For 2g/L dopamine solution.Secondly, PE film is soaked in above-mentioned solution, at 20 DEG C of continuously stirred 24h.Finally, PE is taken out thin
Film ethanol solution and deionized water exchange are cleaned for several times, successfully prepare the PE perforated membrane with poly-dopamine coating.
2012, Rim, N.G. etc. utilized poly-dopamine that Poly-L-lactic acid (PLLA) electrospun fibers film is carried out surface
Modified.First, the PLLA taking certain mass is dissolved in dichloromethane and tetrafluoroethene (8:2) mixed solution, acquisition 3% of vibrating
(w/v) PLLA homogeneous solution.Secondly, being 13-15kV at voltage, collection distance is 25cm, and advancing speed is the condition of 2mL/h
Under carry out electrostatic spinning, obtain PLLA fibrous membrane.Finally, 8cm × 8cmPLLA fibrous membrane is immersed in dopamine solution (2g/L,
PH=8.5), in, take out after continuously stirred 1h and clean repeatedly with deionized water, obtaining the PLLA fibrous membrane of poly-dopamine coating.
Result shows: the PLLA fibrous membrane of poly-dopamine coating has preferable hydrophilic and cell compatibility.
Summary of the invention
For prior art and material above shortcomings, it is an object of the invention to provide a kind of biologic bracket material.
Be the technical scheme is that by realizing the purpose of the present invention
A kind of high-modulus super hydrophilic biological support preparation method, comprises the following steps:
Step one) preparation of nano-cellulose suspension: chloroform and dimethylformamide are mixed with the mass ratio of 3:1,
Add nano-cellulose, after mixing, obtain nano-cellulose suspension；
Step 2) synthesis macromolecule/nano cellulose blend spinning liquid preparation: by biological degradable synthesized polymer material
Being dissolved in nano-cellulose suspension, under room temperature, concussion is dissolved, it is thus achieved that synthesis macromolecule/nano cellulose blend spinning liquid；
Step 3) electrostatic spinning preparation synthesis high polymer nanometer fiber element composite fibre: will synthesis macromolecule/nano fiber
Element blend spinning liquid loads electrostatic spinning apparatus, is connected, the syringe needle connecting syringe with the aluminum of ground connection with high-voltage generator
Foil paper, for receiving device, prepares composite cellulosic membrane, is placed in exsiccator storage；
Step 4) pre-wet process: composite cellulosic membrane is cut into and is fixed on metal rack, keep its flat presentation, so
After metal rack is put in pre-wetted liquid, vibrate 1h；
Step 5) configuration Tris-HCl buffer: trishydroxymethylaminomethane is dissolved in deionized water, then is added dropwise over dilute
Salt acid for adjusting pH value, to 8.5, is demarcated with deionized water, is obtained Tris-HCl buffer；
Step 6) poly-dopamine coating: being joined by dopamine in Tris-HCl buffer, preparation concentration is that 2g/L is many
Bar amine aqueous solution, is immersed in metal rack in dopamine solution, and magnetic agitation 8h is taken out, and cleans with deionized water, does in vacuum
It is dried in dry case, gets product.
Further improve, described step 2) in, biological degradable synthesized polymer is polylactic acid or polyurethane.
Further improving, the voltage of electrostatic spinning is 15-16kV, and receiving range is 15cm, and air humidity is 40%.
Further improve, the internal diameter 0.8mm of syringe needle.
Further improve, pre-wetted liquid be mass fraction be the ethanol water of 50%.
Compared with prior art, advantages of the present invention has:
(1) this method preparation synthesis macromolecule/nano cellulose composite fiber, the advantage combining bi-material so that it is
While there is excellent mechanical performances, there is good hydrophilicity and biocompatibility.
(2) introducing of nano-cellulose, strengthens poly-dopamine at composite fibrous scaffold surface modification effect, it is thus achieved that coating is equal
The modified composite fiber of one.
(3) use poly-dopamine modified to composite fibrous coating, improve the hydrophilicity of biological support, mechanical property further
And the adhesion of biocompatibility, beneficially cell, can breed and break up.
Accompanying drawing explanation
SEM figure before and after PLA/CNF composite fibrous scaffold coating in Fig. 1 embodiment 1；
SEM figure before and after TPU/CNF composite fibrous scaffold coating in Fig. 2 embodiment 2.
Detailed description of the invention:
Describe or describe the present invention by the following examples in detail rather than limit the invention.
Embodiment 1:
A kind of preparation method based on polylactic acid (PLA)/nano-cellulose (CNF) composite fibrous scaffold, including making as follows
Standby step:
Step one) chloroform and dimethylformamide are mixed with the mass ratio of 3:1, weigh 0.066g nano-cellulose
(CNF) chloroform-dimethylformamide mixed solution of 20mL, magnetic agitation 3h, supersound process 30min, it is thus achieved that homogeneous it are dissolved in
CNF suspension.
Step 2) weigh 2.2g PLA and be dissolved in above-mentioned steps one) the CNF suspension for preparing, shaken at room temperature 6h, it is thus achieved that
PLA/CNF blend spinning solution, wherein PLA concentration is 10% (w/v), and it is 3% (w/w) that CNF accounts for the mass ratio of PLA.
Step 3) by above-mentioned steps two) the PLA/CNF blend spinning liquid prepared loads with the 5mL of spinning syringe needle poly-four
Fluorothene syringe, syringe is installed on SN-50 micro-injection pump and arrange propelling speed be 0.9mL/h, by connections injection
The syringe needle (internal diameter 0.8mm) of device is connected with high-voltage generator, and with the aluminium-foil paper of ground connection for reception device, spinning voltage is
16kV, receiving range are 15cm, spinning temperature be room epidemic disaster be 40%, spinning time 3h, prepare PLA/CNF be combined
Fibrous membrane, and store stand-by in exsiccator.
Step 4) by step 3) the PLA/CNF composite cellulosic membrane for preparing is cut into 8cm × 8cm, is fixed on metal
On support so that it is keeping good its flat presentation on support, the mixing that then itself and support are put into second alcohol and water (5:5) is molten
In liquid, magnetic force vibration 1h.
Step 5) weigh 0.3028g trishydroxymethylaminomethane (Tris) and be dissolved in 250mL deionized water, stirring makes it complete
CL, the most dropwise to 8.5, finally uses deionized water mark toward addition 1mol/L dilute hydrochloric acid regulation solution ph in Tris solution
Fixed, it is thus achieved that the Tris-HCl buffer of 1L.
Step 6) first the dopamine of 0.5g is joined in 250mL Tris-HCl buffer, preparation concentration is 2g/L
Dopamine solution.Secondly the PLA/CNF composite fibrous scaffold that pre-wets obtained in step 5 is taken out and to be immersed in dopamine molten
In liquid, take out sample after magnetic agitation 8h, clean 3 times with deionized water, dried for standby in vacuum drying oven.
In order to better illustrate the present invention, in conjunction with accompanying drawing, introduce the PLA/ of the embodiment of the present invention 1 gained poly-dopamine coating
CNF composite fibrous scaffold.
Fig. 1 is that the SEM of the PLA/CNF composite fibrous scaffold before and after poly-dopamine coating schemes [before coating (a)；After coating
(b)】.It can be seen that compared with composite fibre before coating, after coating, composite fiber surface roughness increases, and diameter
Substantially increase, it addition, in the cracks (arrow indication) of poly-dopamine it can be seen that poly-dopamine has been coated with compound fibre completely
Dimension, thus define nucleocapsid structure.Result shows: the poly-dopamine of PLA/CNF composite fiber surface successful application.
Table 1 gives PLA, PLA/CNF and the water contact angle of poly-dopamine coating PLA/CNF fibrous membrane, permissible from figure
Finding out, PLA fiber water contact angle is 137.8 ° ± 1.5, and PLA/CNF composite cellulosic membrane water contact angle be reduced to 130.6 ° ±
1.5, further, since the composite fibre total hydrophilic after poly-dopamine coating causes its water contact angle to measure.Result shows:
The introducing of CNF improves PLA Hydrophilic Fiber energy, and poly-dopamine is coating modified further increases composite fibre hydrophilic
Can so that it is become super hydrophilic biological support.
Table 1 PLA, PLA/CNF and poly-dopamine coating PLA/CNF fibrous membrane water contact angle
Embodiment 2:
A kind of preparation method based on polyurethane (TPU)/nano-cellulose (CNF) composite fibrous scaffold, including walking as follows
Rapid:
Step one) chloroform and dimethylformamide are mixed with the mass ratio of 3:1, weigh 0.008g nano-cellulose
(CNF) the chloroform dimethylformamide mixed solution of 20mL, magnetic agitation 3h, supersound process 30min, it is thus achieved that homogeneous it are dissolved in
CNF suspension.
Step 2) weigh 1.6g TPU and be dissolved in above-mentioned steps one) the CNF suspension for preparing, shaken at room temperature 6h, it is thus achieved that
TPU/CNF blend spinning solution, wherein TPU concentration is 8% (w/v), and it is 0.5% (w/w) that CNF accounts for the mass ratio of TPU.
Step 3) by above-mentioned steps two) the TPU/CNF blend spinning liquid prepared loads with the 5mL of spinning syringe needle poly-four
Fluorothene syringe, syringe is installed on SN-50 micro-injection pump and arrange propelling speed be 0.5mL/h, by connections injection
The syringe needle (internal diameter 0.8mm) of device is connected with high-voltage generator, and with the aluminium-foil paper of ground connection for reception device, spinning voltage is
15kV, receiving range are 15cm, spinning temperature be room epidemic disaster be 40%, spinning time 3h, prepare TPU/CNF be combined
Fibrous membrane, and store stand-by in exsiccator.
Step 4) by step 3) the TPU/CNF composite cellulosic membrane for preparing is cut into 8cm × 8cm, is fixed on metal
On support so that it is keeping good its flat presentation on support, the mixing that then itself and support are put into second alcohol and water (5:5) is molten
In liquid, magnetic force vibration 1h.
Step 5) weigh 0.3028g trishydroxymethylaminomethane (Tris) and be dissolved in 250mL deionized water, stirring makes it complete
CL, the most dropwise to 8.5, finally uses deionized water mark toward addition 1mol/L dilute hydrochloric acid regulation solution ph in Tris solution
Fixed, it is thus achieved that the Tris-HCl buffer of 1L.
Step 6) first the dopamine of 0.5g is joined in 250mL Tris-HCl buffer, preparation concentration is 2g/L
Dopamine solution.Secondly the TPU/CNF composite fibrous scaffold that pre-wets obtained in step 5 is taken out and to be immersed in dopamine molten
In liquid, take out sample after magnetic agitation 8h, clean 3 times with deionized water, dried for standby in vacuum drying oven.
In order to better illustrate the present invention, in conjunction with accompanying drawing, introduce the TPU/ of the embodiment of the present invention 2 gained poly-dopamine coating
CNF composite fibrous scaffold.
Fig. 2 is that the SEM of TPU/CNF composite fibrous scaffold schemes [before coating (a) before and after poly-dopamine coating；After coating (b)；
Coating amplifies (c)；Coating stretching (d)].From Fig. 2 (a, b) in it can be seen that compared with composite fibre before coating, compound after coating
Fiber surface roughness increases, and diameter substantially increases.From Fig. 2, (c, d) it can be seen that (scheme after particularly sample stretches
2d), composite fiber surface occurs in that relatively multiple cracks (arrow indication), and this is owing under the stretching action of power, poly-dopamine is coated with
Produced by fault rupture.Therefore, result shows: poly-dopamine success and be coated with TPU/CNF composite fibre completely, thus shape
Become nucleocapsid structure.
Claims (5)
1. a high-modulus super hydrophilic biological support preparation method, it is characterised in that comprise the following steps:
Step one) preparation of nano-cellulose suspension: chloroform and dimethylformamide are mixed with the mass ratio of 3:1, then adds
Enter nano-cellulose, after mixing, obtain nano-cellulose suspension；
Step 2) synthesis macromolecule/nano cellulose blend spinning liquid preparation: biological degradable synthesized polymer material is dissolved in
Nano-cellulose suspension, under room temperature, concussion is dissolved, it is thus achieved that synthesis macromolecule/nano cellulose blend spinning liquid；
Step 3) electrostatic spinning preparation synthesis high polymer nanometer fiber element composite fibre: synthesis macromolecule/nano cellulose is mixed
Close spinning liquid and load electrostatic spinning apparatus, the syringe needle connecting syringe is connected with high-voltage generator, with the aluminium-foil paper of ground connection
For receiving device, prepare composite cellulosic membrane, be placed in exsiccator storage；
Step 4) pre-wet process: composite cellulosic membrane is reduced and is fixed on metal rack, keep its flat presentation, then by gold
Belonging to support and put in pre-wetted liquid, vibrate 1h；
Step 5) configuration Tris-HCl buffer: trishydroxymethylaminomethane is dissolved in deionized water, then is added dropwise over dilute hydrochloric acid
Regulation pH value, to 8.5, is demarcated with deionized water, is obtained Tris-HCl buffer；
Step 6) poly-dopamine coating: being joined by dopamine in Tris-HCl buffer, preparation concentration is the dopamine of 2g/L
Solution, is immersed in metal rack in dopamine solution, and magnetic agitation 8h is taken out, and cleans with deionized water, at vacuum drying oven
Interior dry, get product.
2. high-modulus super hydrophilic biological support preparation method as claimed in claim 1, it is characterised in that described step 2) in,
Biological degradable synthesized polymer is polylactic acid or polyurethane.
3. high-modulus super hydrophilic biological support preparation method as claimed in claim 1, it is characterised in that described step 3) in,
The voltage of electrostatic spinning is 15-16kV, and receiving range is 15cm, and air humidity is 40%.
4. high-modulus super hydrophilic biological support preparation method as claimed in claim 1, it is characterised in that described step 3) in,
The internal diameter 0.8mm of syringe needle.
5. high-modulus super hydrophilic biological support preparation method as claimed in claim 1, it is characterised in that described step 4) in,
Pre-wetted liquid be mass fraction be the ethanol water of 50%.
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| CN108744036A (en) * | 2018-06-22 | 2018-11-06 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method of poly-dopamine modified lithium Electrospun PDLLA-PCL-PDLLA fiber sacculus and products thereof and application |
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| CN110302420A (en) * | 2019-06-27 | 2019-10-08 | 福建工程学院 | A kind of preparation method of in-situ polymerization PDA coating surface modified biological bracket |
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| CN110302420A (en) * | 2019-06-27 | 2019-10-08 | 福建工程学院 | A kind of preparation method of in-situ polymerization PDA coating surface modified biological bracket |
| CN110508253A (en) * | 2019-06-27 | 2019-11-29 | 福建工程学院 | A kind of preparation method of nanofiber adsorbed film |
| CN111634091A (en) * | 2020-04-17 | 2020-09-08 | 上海工程技术大学 | Unidirectional moisture-conducting textile composite material with Janus structure and preparation method thereof |
| CN112169019A (en) * | 2020-09-10 | 2021-01-05 | 南方医科大学 | Direct-writing forming 3D printing biological ink and preparation method thereof |
| CN112169019B (en) * | 2020-09-10 | 2021-11-02 | 南方医科大学 | Direct-writing forming 3D printing biological ink and preparation method thereof |
| CN113403846A (en) * | 2021-07-30 | 2021-09-17 | 南昌大学附属口腔医院(江西省口腔医院) | Polylactic acid nanofiber composite membrane with biomimetic mineralization and antibacterial functions and preparation method and application thereof |
| CN114958713A (en) * | 2022-05-31 | 2022-08-30 | 杭州赫贝科技有限公司 | Three-dimensional cell culture support of tubular structure |
| CN114958713B (en) * | 2022-05-31 | 2024-02-20 | 杭州赫贝科技有限公司 | Three-dimensional cell culture support of tubular structure |
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