CN106267174A - The preparation method of M1 macrophage tumor vaccin - Google Patents
The preparation method of M1 macrophage tumor vaccin Download PDFInfo
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- CN106267174A CN106267174A CN201610634205.4A CN201610634205A CN106267174A CN 106267174 A CN106267174 A CN 106267174A CN 201610634205 A CN201610634205 A CN 201610634205A CN 106267174 A CN106267174 A CN 106267174A
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Abstract
The present invention relates to the preparation method of M1 macrophage tumor vaccin, belong to immunotherapy of tumors technical field.The present invention comprises and prepares tumor cell lysis liquid, carries out the differentiation culture of macrophage, and carries out the M1 polarization induction of macrophage.The invention have the benefit that M1 type macrophage is the main cleaning cell of body pathogenic microorganism and slough, and there is antigen presentation, play a crucial role in the induction and process of immune regulation of specific immune response.Use necrotic tumor cells to stimulate M1 macrophage and the M1 macrophage tumor vaccin prepared, tumor cell will can be overcome to express drawback not enough, that antigenicity is weak because of surface antigen, there is good induction specificity antineoplastic immunity reagentia.
Description
Technical field
The present invention relates to immunotherapy of tumors technical field, the preparation method of particularly M1 macrophage tumor vaccin.
Background technology
Specificity active immunotherapy treatment tumor has certain curative effect and for many institutes confirms how to prepare
The tumor vaccine of high antigen presentation is the key improving this therapy curative effect.At present, the tumor vaccine being in basis and clinical investigation phase is main
There are the autologous tumor cell tumor vaccine of modification, single protein ingredient tumor vaccine or heat shock protein peptide complex tumor vaccine and dendritic cell
Tumor vaccine etc., such as patent 93112060.8 etc..The autologous tumor cell tumor vaccine modified is to use suitable chemical factors thin to tumor
Born of the same parents carry out processing to promote allosteric or the coupling of cell cortex protein, or make tumor thin by the means such as virus transfection and transgenic
The foreign body increase of born of the same parents, the tumor vaccine prepared to improve the immunogenicity of tumor cell, though tumor cell is after treatment necessarily
The antigenicity of tumor cell can be increased in degree, but method of modifying the most fundamentally changes tumor cell surface MHC1 quasi-molecule
Expressing and reduce, tomour specific or heterogenetic antigen can not express the drawback at cell surface, autologous modification tumor cell tumor vaccine
Immunoreactive fall out effect is not strong, and clinical efficacy is the most very good.
Single protein ingredient tumor vaccine (tumor vaccine as prepared with MAGE1, MAGE2 of extracting from melanoma cell),
These tumor vaccine can not overcome the heterogeneity of tumor, uses the treatment of such tumor vaccine that immune system only can be made to be somebody's turn to do expressing in solid tumor
Antigen tumor cell produces and kills, and will not kill the tumor cell not expressing this antigen in same tumor, and single albumen becomes
The clinical efficacy dividing tumor vaccine is the most undesirable.
Heat shock protein peptide complex tumor vaccine is to utilize isolated heat shock protein peptide from tumor cell to be combined system
Standby tumor vaccine, the tumor vaccine of this protein ingredient can overcome the restriction of MHCI quasi-molecule to can be used in allogeneic, and energy
Enough overcoming the heterogeneity of tumor, experimentation shows that this tumor vaccine has a preferable tumor-inhibiting action, but being prepared as of this tumor vaccine
This is the highest, tumor vaccine cell quantity is few, is not easy to clinical practice application.
Dendritic cell tumor vaccine (DC Cell vaccine) is the tumor vaccine of hot research the most both at home and abroad, DC glucagonoma
Seedling is to utilize dendritic cell and tumor thin or tumor cell content mixed culture and the Tumor vaccine prepared, these tumor Seedlings
Though it is weak and cannot be used for allochthonous drawback that tumor vaccine can overcome tumor-cell antigen, but heat shock protein peptide complex and
The separation and Extraction of DC precursor is more complicated, and costly, is unsuitable for clinical practice.This invention is to have for setting up one
Imitate and be easy to the method for preparing tumour vaccinum of clinical practice.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, the present invention to provide a kind of M1 macrophage tumor vaccin
Preparation method.The present invention solves it and technical problem is that and take techniques below scheme to realize: the system of M1 macrophage tumor vaccin
Preparation Method, comprises the steps of inducing tumor cell necrosis and prepares lysate, carrying out the differentiation of Monocyte-macrophages
Cultivating, the M1 polarization induction and the tumor cell lysis liquid that carry out macrophage stimulate M1 macrophage.
Preferably, described tumor cell lysis liquid step of preparing comprises the steps of grinding tumor tissues and carries out once
It is filtrated to get filtrate;Described filtrate is joined in the test tube equipped with Ficoll-Hypaque lymphocyte separation medium, 2000rpm
Centrifugal 20min, draws mononuclearcell layer and obtains tumor cell, be moved in another test tube by described tumor cell;Physiology salt
Tumor cell described in aqueous suspension, 1000rpm is centrifuged 10min, abandons supernatant, in triplicate;With normal saline by described tumor cell
Furnishing 4 × 107C/mL, 37 DEG C, 5%CO2Under the conditions of place 48 hours prepared cell suspension;Described cell suspension is placed in-80 DEG C
Refrigerator 25min, takes out and puts into rapidly 10min in 37 DEG C of water-baths, 3 times repeatedly;Described cell suspension 1000rpm is centrifuged 5min,
Take supernatant, be dispensed in centrifuge tube ,-20 DEG C of preservations.
Preferably, the differentiation culture step of described macrophage comprises the steps of and heparin anti-coagulating is injected centrifuge tube
In, at 4 DEG C, 1000rpm is centrifuged 5min, abandons upper plasma and obtains hemocyte;Hank ' s liquid or physiology is added in described hemocyte
Blood cell suspension is made in saline mixing, and described blood cell suspension is slowly added into the centrifuge tube equipped with Ficoll-Hypaque
In, at 4 DEG C, 2000rpm is centrifuged 20min;Take PBMC and move it in centrifuge tube, adding Hank ' s liquid or normal saline, 4
At DEG C, 1000rpm is centrifuged 5min, abandons supernatant, washes cell 3 times with Hank ' s liquid or normal saline;Add anteserum-less substrate suspension institute
State cell, cell suspension is added in culture bottle, 37 DEG C, 5%CO2Under the conditions of cultivate 2 hours;Jiggling culture bottle, suction is abandoned
Non-adherent cell.
Preferably, the M1 polarization induction step of described macrophage comprises the steps of the serum-free added containing GM-CSF
Pei Ji, 37 DEG C, 5%CO2Under the conditions of cultivate 9 days;Add and contain IFN-γ and the anteserum-less substrate of tumor cell lysis liquid, 37
DEG C, 5%CO2Under the conditions of cultivate 3 days;Jiggle culture bottle, inhale and abandon Pei Ji;Use normal saline flushing attached cell, scrape patch
Parietal cell, goes to, in centrifuge tube, add saline, and at 4 DEG C, 1000rpm is centrifuged 5min, supernatant discarded;Normal saline is washed cell and is laid equal stress on
Outstanding cell, adds CpG, filters, is loaded by cell suspension in cryopreservation tube, seals adhesive label.
Advantages of the present invention and good effect be: M1 type macrophage is the main of body pathogenic microorganism and slough
Cleaning cell, and there is antigen presentation, play a crucial role in the induction and process of immune regulation of specific immune response.
Use necrotic tumor cells lysate to stimulate M1 macrophage and the M1 macrophage tumor vaccin prepared, tumor can be overcome
Cell expresses drawback not enough, that antigenicity is weak because of surface antigen, has good induction specificity antineoplastic immunity reagentia.
Accompanying drawing explanation
Fig. 1 is M1 type macrophage degree CD68, the expression rate of CD14 molecule;
Fig. 2 is M1 type macrophage degree CD68, the expression rate of MHC II molecule;
Fig. 3 is M1 type macrophage degree CD80, the expression rate of CD86 molecule;
Fig. 4 is the handling process of experimental mice;
Fig. 5 is the tumor formation rate of each group of mice;
Fig. 6 is the gross tumor volume of each group of mice;
Fig. 7 is the tumor weight of each group of mice;
Fig. 8 is the tumor splenocyte tumor killing rate of each group of mice.
Detailed description of the invention
Below in conjunction with the accompanying drawings, by specific embodiment, the invention will be further described.Following example are descriptive
, it not determinate, it is impossible to limit protection scope of the present invention with this.
Experiment equipment
Low temperature normal speed centrifuge (U.S., Beckman), CO2Cell culture incubator (Japan, Sanyo), pipettor (U.S.,
Thermo), measuring pipette (U.S., Kimble), 25cm2Culture bottle (U.S., Corning), 15ml centrifuge tube (U.S.,
Corning), 50ml centrifuge tube (U.S., Corning), cell scraper (U.S., Corning), piston (China, Shandong prestige
High medical high polymer limited company), culture dish (U.S., Corning), cellular filter (U.S., Corning), be inverted aobvious
Micro mirror (Japan, Nikon), cryopreservation tube (U.S., Corning).
Experiment reagent
Ficoll-Hypaque cell separation liquid (Hao ocean, Tianjin biological product science and technology limited Company)), sodium chloride note
Penetrate liquid (Shijiazhuang four medicine company), NaCl (examination of traditional Chinese medicines Lu), KCl (examination of traditional Chinese medicines Lu), MgSO4·7H2(it is limited that Tianjin causes remote chemistry to O
Company), MgCl2·6H2O (Tianjin Zhi Yuan Chemical Co., Ltd.), CaCl2(Tianjin Bo Di Chemical Co., Ltd.), Na2HPO4·
12H2O (Tianjin recovery development in science and technology company limited), KH2PO4(Tianjin Zhi Yuan Chemical Co., Ltd.), glucose (traditional Chinese medicines Lu
Examination), phenol red (upper the graceful bio tech ltd of Hypon), heparin sodium (Shanghai No.1 Bio-Chemical Pharmacetical Industry Co., Ltd), IFN-γ (north
Fourth Ring, capital Biology Pharmacy Co., Ltd), GM-CSF (Beijing Shuanglu Pharmaceutical Co., Ltd.), anteserum-less substrate (Thermo),
CpG(Peprotech)。
The preparation of tumor cell lysis liquid: fresh tumor tissue (in vitro 0.5 hour) is cut into 1mm3Square, after washing,
Using piston tumor mass to be ground in culture dish, 200 mesh filter screens filter, and filtrate joins the Ficoll-equipped with dilution
In Hypaque test tube [density of Ficoll-Hypaque is 1.077 ± 0.02): normal saline=3:1], 2000rpm is centrifuged
20min, draws mononuclearcell layer, is moved into by tumor cell in another test tube.Normal saline suspension cell, 1000rpm from
Heart 10min, abandons supernatant, in triplicate.With normal saline by cell furnishing 4 × 107C/mL, 37 DEG C, 5%CO2Under the conditions of place 48
Hour, cell suspension is placed in-80 DEG C of refrigerator 25min, takes out and put into rapidly 10min in 37 DEG C of water-baths, 3 times repeatedly.1000rpm
Centrifugal 5min, takes supernatant, is dispensed in centrifuge tube ,-20 DEG C of preservations.
Configuration Hank ' s liquid: 1. solution A: NaCl 160g, KCl8g, MgSO4·7H2O 2g、MgCl2·6H2O 2g adds
To 800ml tri-distilled water, dissolve.CaCl22.8g is dissolved in 100ml tri-distilled water, joins in above-mentioned 800ml solution, supply after dissolving
Tri-distilled water is to 1000ml.2. second liquid: Na2HPO4·12H2O 3.04g、KH2PO41.2g, glucose 20g add 800ml distilled water,
Dissolve.Add 0.4% phenol red solution 100ml, above-mentioned two liquid mixing, add tri-distilled water to 1000ml.By solution A 1 part, second during use
Liquid 1 part, tri-distilled water 18 parts filtration, 8 pounds of sterilizings in 20min minute, 4 DEG C of preservations.
The differentiation culture of macrophage is with M1 polarization induction: injected by 20ml heparin anti-coagulating in 50ml centrifuge tube, at 4 DEG C
Lower 1000rpm is centrifuged 5min.Upper plasma is abandoned in shifting, adds Hank ' s liquid or normal saline to 20ml, mixing.Blood cell suspension is delayed
Slowly joining in the centrifuge tube equipped with 4ml Ficoll-Hypaque (density: 1.077 ± 0.001), often pipe 10ml, at 4 DEG C
2000rpm is centrifuged 20min, draws precipitation (PBMC layer) cell and moves it in 15ml centrifuge tube.Add Hank ' s liquid or physiology
Saline is to 15ml, and at 4 DEG C, 1000rpm is centrifuged 5min, abandons supernatant.Hank ' s liquid or normal saline wash cell 3 times.Add 16ml
Anteserum-less substrate suspension PBMC cell, joins 25cm by cell suspension2In culture bottle, every bottle of 8ml cell suspension, 37 DEG C, 5%
CO2Under the conditions of cultivate 2 hours.Jiggle culture bottle, inhale and abandon non-adherent cell.Add the serum-free containing 100ng/mlGM-CSF
Pei Ji, every bottle of 12ml, 37 DEG C, 5%CO2Under the conditions of cultivate 9 days.Add containing 100IU/ml IFN-γ and 100 μ l/ml tumors thin
The anteserum-less substrate of cellular lysate liquid (preparation method such as method 1), every bottle of 4ml, 37 DEG C, 5%CO2Under the conditions of cultivate 3 days.Draw
2ml supernatant is used for mycoplasma inspection (RT-PCR method), then jiggles culture bottle, inhales and abandons Pei Ji.Use normal saline flushing
Attached cell 3 times, scrapes attached cell with cell scraper, goes in centrifuge tube, adds normal saline to 50mL.At 4 DEG C
1000rpm is centrifuged 5min, supernatant discarded.Normal saline washes cell 3 times.1ml normal saline re-suspended cell, adds 4mg/ml's
0.1mlCpG, 200 mesh filter screens filter, are encased in by cell suspension in 2mL cryopreservation tube, and sealing, adhesive label, for notch graft
Kind.
Measure of merit: in Fig. 5 to Fig. 8, A is physiological saline group;B is macrophage inoculation group;C is macrophage
Tumor vaccination group.A, B and C group mice respectively at left hind subcutaneous vaccination normal saline, without tumor stimulate huge bite thin
Born of the same parents and macrophage tumor vaccin (downright bad H22Tumor cell stimulates M1 macrophage to obtain), 0.1ml/ only (inoculate with C group by B group
Macrophage concentration be 2 × 106/ ml, normal saline dilution), 1 times a week, continuous 2 times, after being spaced 2 weeks, four groups of mices are respectively
In the well-grown H of left fore subcutaneous vaccination22Tumor cell, only (concentration of cell suspension is 2 × 10 to 0.1ml/6/ ml, physiology salt
Water dilutes), tumor cell inoculation puts to death whole mice after 4 weeks, and calculates four groups of mice tumor formation rates, electronic balance weighing tumor respectively
Weight, the length (L) of vernier caliper measurement tumor nodule and width (W), calculate tumor volume.Note (: volume=0.5 × L × W2)。
After result display macrophage tumor vaccin inoculation group tumor cell attack, tumor tumor formation rate, tumor volume and tumor weight are significantly lower than
Non-stimulating expression of macrophage inoculation group and normal saline inoculation group (P < 0.05).The splenocyte pair of macrophage tumor vaccin inoculation group
The killing rate of tumor cell, apparently higher than non-stimulating expression of macrophage inoculation group and normal saline inoculation group (P < 0.05), shows huge biting
Cell tumour vaccine can induce specificity antineoplastic immunity reaction in vivo.
Claims (4)
- The preparation method of 1.M1 macrophage tumor vaccin, it is characterised in that comprise the steps of inducing tumor cell downright bad and Preparing lysate, carry out the differentiation culture of Monocyte-macrophages, the M1 polarization induction and the tumor that carry out macrophage are thin Cellular lysate liquid stimulates M1 macrophage.
- The preparation method of M1 macrophage tumor vaccin the most according to claim 1, it is characterised in that described induced tumor Necrocytosis and to prepare lysate step specific as follows:Grind tumor tissues and be once filtrated to get filtrate;Described filtrate being joined in the test tube equipped with Ficoll-Hypaque lymphocyte separation medium, 2000rpm is centrifuged 20min, draws mononuclearcell layer and obtains tumor cell, be moved in another test tube by described tumor cell;With the normal saline described tumor cell of suspension, 1000rpm is centrifuged 10min, abandons supernatant, in triplicate;With normal saline by described tumor cell furnishing 4 × 107C/mL, 37 DEG C, 5%CO2Under the conditions of place 48 hours prepare necrosis Cell suspension;Described cell suspension is placed in-80 DEG C of refrigerator 25min, takes out and put into rapidly 10min in 37 DEG C of water-baths, 3 times repeatedly;Described cell suspension 1000rpm is centrifuged 5min, takes supernatant, be dispensed in centrifuge tube ,-20 DEG C of preservations.
- The preparation method of M1 macrophage tumor vaccin the most according to claim 1, it is characterised in that described macrophage Differentiation culture step comprise the steps ofBeing injected by heparin anti-coagulating in centrifuge tube, at 4 DEG C, 1000rpm is centrifuged 5min, removes upper plasma and obtains hemocyte;In described hemocyte, add Hank ' s liquid or normal saline and blood cell suspension is made in mixing, described blood cell suspension is delayed Slowly joining equipped with in the centrifuge tube of Ficoll-Hypaque, at 4 DEG C, 2000rpm is centrifuged 20min;Taking PBMC and move it in centrifuge tube, adding Hank ' s liquid or normal saline, at 4 DEG C, 1000rpm is centrifuged 5min, abandons Clearly, cell is washed 3 times with Hank ' s liquid or normal saline;Add the anteserum-less substrate described cell of suspension, cell suspension is added in culture bottle, 37 DEG C, 5%CO2Under the conditions of cultivate 2 little Time;Jiggle culture bottle, inhale and abandon non-adherent cell.
- The preparation method of M1 macrophage tumor vaccin the most according to claim 1, it is characterised in that described macrophage M1 polarization induction step comprise the steps ofInhale to abandon and the culture bottle of non-adherent cell adds the anteserum-less substrate containing GM-CSF, 37 DEG C, 5%CO2Under the conditions of cultivate 9 days;Add containing IFN-γ and the anteserum-less substrate of tumor cell lysis liquid, 37 DEG C, 5%CO2Under the conditions of cultivate 3 days;Jiggle culture bottle, inhale and abandon Pei Ji;Use normal saline flushing attached cell, scrape attached cell, go to, in centrifuge tube, add saline, at 4 DEG C 1000rpm from Heart 5min, supernatant discarded;Normal saline washes cell re-suspended cell, adds CpG, filters, is loaded by cell suspension in cryopreservation tube, seals label adhering Sign.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109609452A (en) * | 2018-12-27 | 2019-04-12 | 青岛麦迪赛斯生物科技有限公司 | A kind of efficient macrophages in vitro preparation method |
WO2020061084A1 (en) * | 2018-09-17 | 2020-03-26 | Los Angeles Biomedical Research Institute At Harbor-Ucla Medical Center | Target-primed macrophages and therapeutic uses thereof |
CN114134122A (en) * | 2021-12-06 | 2022-03-04 | 大连大学 | Macrophage-mediated mesenchymal stem cell directed differentiation culture method |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1413731A (en) * | 2002-09-17 | 2003-04-30 | 袁小林 | Macrophage tumor vaccin and its preparation method |
CN1609199A (en) * | 2004-11-17 | 2005-04-27 | 哈尔滨医科大学 | Macrophage in vitro inducing culture method |
CN103958546A (en) * | 2011-10-21 | 2014-07-30 | 特朗斯吉有限公司 | Modulation of macrophage activation |
-
2016
- 2016-08-04 CN CN201610634205.4A patent/CN106267174A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1413731A (en) * | 2002-09-17 | 2003-04-30 | 袁小林 | Macrophage tumor vaccin and its preparation method |
CN1609199A (en) * | 2004-11-17 | 2005-04-27 | 哈尔滨医科大学 | Macrophage in vitro inducing culture method |
CN103958546A (en) * | 2011-10-21 | 2014-07-30 | 特朗斯吉有限公司 | Modulation of macrophage activation |
Non-Patent Citations (3)
Title |
---|
ALESSANDRA NARDIN等: "Macrophages and cancer", 《FRONTIERS IN BIOSCIENCE》 * |
张春蕾等: "死亡肿瘤细胞诱导分化的巨噬细胞体内抗瘤作用的研究", 《国际免疫学杂志》 * |
袁小林等: "巨噬细胞瘤苗免疫调节作用的研究", 《国际免疫学杂志》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2020061084A1 (en) * | 2018-09-17 | 2020-03-26 | Los Angeles Biomedical Research Institute At Harbor-Ucla Medical Center | Target-primed macrophages and therapeutic uses thereof |
CN109609452A (en) * | 2018-12-27 | 2019-04-12 | 青岛麦迪赛斯生物科技有限公司 | A kind of efficient macrophages in vitro preparation method |
CN114134122A (en) * | 2021-12-06 | 2022-03-04 | 大连大学 | Macrophage-mediated mesenchymal stem cell directed differentiation culture method |
CN114134122B (en) * | 2021-12-06 | 2024-05-28 | 大连大学 | Macrophage-mediated mesenchymal stem cell directional differentiation culture method |
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