CN106265735A - Functionalized multi-wall carbonnanotubes application in preparing medicine for anti transfer of tumor - Google Patents

Functionalized multi-wall carbonnanotubes application in preparing medicine for anti transfer of tumor Download PDF

Info

Publication number
CN106265735A
CN106265735A CN201610651752.3A CN201610651752A CN106265735A CN 106265735 A CN106265735 A CN 106265735A CN 201610651752 A CN201610651752 A CN 201610651752A CN 106265735 A CN106265735 A CN 106265735A
Authority
CN
China
Prior art keywords
tumor
functionalized multi
wall carbonnanotubes
application
lung
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610651752.3A
Other languages
Chinese (zh)
Inventor
杨静
吴练练
梁峥琰
夏煜琦
徐阳
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wuhan University WHU
Original Assignee
Wuhan University WHU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wuhan University WHU filed Critical Wuhan University WHU
Priority to CN201610651752.3A priority Critical patent/CN106265735A/en
Publication of CN106265735A publication Critical patent/CN106265735A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/44—Elemental carbon, e.g. charcoal, carbon black
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
    • A61K47/38—Cellulose; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
    • A61K9/0092—Hollow drug-filled fibres, tubes of the core-shell type, coated fibres, coated rods, microtubules or nanotubes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Nanotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Dispersion Chemistry (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention discloses the new application of a kind of functionalized multi-wall carbonnanotubes (Carboxylated multiwalled carbon nanotube) anti metastasis.The important point of the present invention is to find that functionalized multi-wall carbonnanotubes can pass through target tumor microenvironment, and reprogramming tumor-associated macrophages (predominantly M2 phenotype) is M1 phenotype, suppresses tumor cell proliferation, to human normal cell's safety, low toxicity.Therefore, have and prepare the application prospect of medicine for anti transfer of tumor.

Description

Functionalized multi-wall carbonnanotubes application in preparing medicine for anti transfer of tumor
Technical field
The invention belongs to field of nanometer material technology, be specifically related to functionalized multi-wall carbonnanotubes in preparing antitumor drug New application.
Background technology
Tumor is to threaten the number one killer of human life.Show according to World Health Organization's investigation, the annual new discovery in the whole world Nearly 14,100,000 people of cancer patient, account for the 1/5 of total death toll.Neoplasm metastasis refers to that cancerous cell transfers to other portions from original position Position also forms the process of new focus, and the tumour patient having more than 90% dies from transfer, is treatment of cancer difficulty, failure and cancer The main cause of patient's high mortality.Therefore, prevent and treat neoplasm metastasis and become an extremely urgent hardship post.
The traditional method for the treatment of neoplasm metastasis has radiation and chemotherapy.But there is the damage of limitation and normal tissue in radiotherapy Property；Generally there is cytotoxic effect in chemotherapy, very serious to the toxic and side effects of liver, kidney, bone marrow and digestive system especially, because of This, constrain they application clinically significantly.Emerging molecular targeted therapy reduces the toxic and side effects of conventional medicament, but The ligandin introduced can increase the immunogenicity of medicine, and medicine controlled releasing problem is also difficult to solve simultaneously, it is difficult to reach preferable Clinical application effect；Gene therapy brings dawn to tumor patient, but its vector construction, carrier and the crosslinking of bullet and at machine The problems such as the change in body distribution, metabolic process are also among inquiring into.
Neoplasm metastasis be unable to do without tumor microenvironment.Tumor microenvironment includes tumor cell, immunocyte, intercellular substance and biological point Sons etc., wherein, tumor-associated macrophages is the important component of tumor microenvironment, and its functional status and density are pernicious with tumor Close dependency is had between degree.Under the stimulation of the microenvironment factor, macrophage can be polarized to suppress the M1 of tumor Type and the M2 type of promotion tumor.M1 type macrophage direct killing tumor also offers the related antigen of tumor, removes tumor；M2 type Macrophage promotes angiogenesis and immunosuppressant, and then promotes the growth of tumor, attacks and shift.Tumor-associated macrophages Predominantly M2 type, how inducing macrophage is polarized to M1 type by M2 is the study hotspot for the treatment of neoplasm metastasis and important target spot.
CNT is a kind of open column shape carbon nanomaterial by the curling of single or multiple lift graphite flake layer, and it is excellent Physicochemical property day by day cause the concern of people.CNT is in multiple sciemtifec and technical spheres such as electronic device, composite and catalysis The aspects such as agent carrier are respectively provided with huge application potential.In field of medical biotechnology, CNT is mainly as a kind of pharmaceutical carrier Study for oncotherapy.Further, it is also possible to the light thermal property utilizing CNT unique directly kills tumor cell, but carbon Nanotube electricity conversion is relatively low and is difficult to promote, and is the big bottleneck restricting its therapeutic effect.Research shows, CNT Easily by monokaryon-macrophage system phagocytosis and assemble wherein.
Summary of the invention
The present inventor, when functionalized multi-wall carbonnanotubes carries out the research of injury of lung, chances on carboxylated Multi-walled carbon nano-tubes can significance suppression Lewis lung cancer and melanoma Lung metastases.Detect discovery, carboxylated many walls carbon further With significance, nanotube can promote that tumor-associated macrophages is reprogrammed as M1 type by M2.Therefore, functionalized multi-wall carbonnanotubes Can be as medicine for anti transfer of tumor.
The technical problem to be solved is to provide a kind of new application of functionalized multi-wall carbonnanotubes, is specifically related to Functionalized multi-wall carbonnanotubes application in preparing medicine for anti transfer of tumor.
Described functionalized multi-wall carbonnanotubes is external diameter 20-30 nm, long 500 nm, the purity nanometer material higher than 98 % Material.
Above-mentioned tumor includes but not limited to pulmonary carcinoma, melanoma.
When specifically applying, directly functionalized multi-wall carbonnanotubes can be used for oncotherapy as medicine, treatment Mode is topical or intravenous injection.
The present invention is by the effect of following experiment proof functionalized multi-wall carbonnanotubes anti metastasis:
Significantly inhibit the transfer of tumor-bearing mice pulmonary to count, hence it is evident that improve tumor-bearing mice body weight；2. promote that lung tumors is correlated with huge Phagocyte is M1 type by M2 reprogramming, increases lung tumors microenvironment M1 type macrophage, reduces M2 type macrophage number simultaneously Amount；3., after chemical rejects mouse lung macrophage, the effect of CNT anti metastasis is reversed.
The invention have the advantages that
1) the functionalized multi-wall carbonnanotubes mature preparation process that the present invention uses is reliable, and raw material sources are extensive and superior in quality.
2) functionalized multi-wall carbonnanotubes has preferable biocompatibility, in the normal tissue through prototype tachymetabolism, Typically will not cause toxic reaction, research report, 5-10 mg/kg functionalized multi-wall carbonnanotubes is essentially without causing mice Toxic reaction.(Qu Guangbo. functionalized multi-wall carbonnanotubes studies on acute toxicity [D] in Mice Body. Shandong University, 2008.)
3) due to solid tumor mass rich blood vessel, blood vessel wall gap is wider and lymphatic return lacks, carboxylated multi-wall carbon nano-tube Pipe is accessible to and is stranded in tumor tissues.Meanwhile, CNT easily by monokaryon-macrophage system phagocytosis thus gathers wherein Collection.Therefore, functionalized multi-wall carbonnanotubes has high targeting to tumor-associated macrophages.
4) functionalized multi-wall carbonnanotubes can be polarized to M1 type by M2 by induced tumor associated macrophages, increases anti-swollen The secretion of tumor inflammatory factor, improves the antineoplastic immune function of body.
5) functionalized multi-wall carbonnanotubes can effectively suppress growth and the transfer of tumor.
6), after functionalized multi-wall carbonnanotubes enters tumor microenvironment, degradation cycle is longer, lasting medicine.
7) using functionalized multi-wall carbonnanotubes itself as medicine, can avoid connecting the Immunogenicity that aglucon brings, Pharmacy cost is greatly lowered simultaneously.
8) tumor-associated macrophages has fixing specialization direction, stable gene, is not likely to produce drug resistance, passes through targeting Macrophage realizes oncotherapy and has good characteristic lasting, efficient.
9) present invention uses conventional administration dosage form, technology maturation, and production technology is simple.
10) present invention uses conventional administration mode, convenient drug administration, no pain.
Accompanying drawing explanation
Fig. 1 functionalized multi-wall carbonnanotubes dose-dependent inhibition Lewis lung cancer Lung metastases.
Fig. 2 functionalized multi-wall carbonnanotubes suppression melanoma Lung metastases.
Fig. 3 functionalized multi-wall carbonnanotubes processes Lewis lung cancer model, B16F10 melanoma tumor model lung tissue Lignum Sappan Essence-Yihong (HE) colored graph.
Fig. 4 functionalized multi-wall carbonnanotubes in Lewis lung cancer model and B16F10 melanoma tumor model to each treated animal The impact of body weight.
Fig. 5 chemical is rejected mouse lung macrophage and is reversed CNT antitumor action.
Fig. 6 immunofluorescence dyeing detection functionalized multi-wall carbonnanotubes is on tumor-associated macrophages polarization impact.
Fig. 7 RT-PCR detection functionalized multi-wall carbonnanotubes is on tumor-associated macrophages polarization impact.
Detailed description of the invention
By combination accompanying drawing described further below it will be further appreciated that the features and advantages of the invention.The enforcement provided Example is only the explanation to the inventive method, and limits remaining content that the present invention discloses never in any form.
Functionalized multi-wall carbonnanotubes used by following example is external diameter 20-30 nm, long 500 nm, and purity is higher than 98 The nano material of %.
[embodiment 1] functionalized multi-wall carbonnanotubes inhibitory action to neoplasm metastasis
(1) mouse pharmacodynamic experiment
1. prepare functionalized multi-wall carbonnanotubes suspension
Functionalized multi-wall carbonnanotubes is dissolved in carboxymethylcellulose sodium solution, concentration 1 mg/ml, ultrasound wave before every time using Concussion.
2. animal model one
1) inoculated tumour cell
Six week old male C57BL/6 mice 36, body weight 18-22g, test first day, every mice equal tail vein injection Lewis Lung carcinoma cell 1 × 105Individual.
2) packet
Postvaccinal mice is randomly divided into 3 groups (model control group, low dosage CNT group, high dose CNT groups), Often group 12.
3) packet transaction
1. model control group: after inoculating 24 hours, tracheal instillation 80 μ l carboxymethylcellulose sodium solution；
2. low dosage CNT group: after inoculating 24 hours, tracheal instillation 40 μ l functionalized multi-wall carbonnanotubes (2 mg/kg)；
3. high dose CNT group: after inoculating 24 hours, tracheal instillation 80 μ l functionalized multi-wall carbonnanotubes (4 mg/kg)；
4) Mouse Weight is claimed every day
5) take lung tissue, observe Lung metastases and count
Injection tumor cell after 14 days, put to death mice, take lung tissue, observe and add up Lung metastases and count.
3. animal model two
1) inoculated tumour cell
Six week old male C57BL/6 mice 24, body weight 18-22g, test first day, every equal tail vein injection of mice B16F10 melanoma cell 2 × 105Individual.
2) packet
Postvaccinal mice is randomly divided into 2 groups (model control group, CNT groups), often group 12.
3) packet transaction
1. model control group: after inoculating 24 hours, tracheal instillation 80 μ l carboxymethylcellulose sodium solution；
2. CNT group: after inoculating 24 hours, tracheal instillation 80 μ l functionalized multi-wall carbonnanotubes (4 mg/kg)；
4) Mouse Weight is claimed every day
5) take lung tissue, observe Lung metastases and count
Injection tumor cell after 14 days, put to death mice, take lung tissue, observe and add up Lung metastases and count.
(2) use hematoxylin-eosin staining method, detect the functionalized multi-wall carbonnanotubes inhibitory action to neoplasm metastasis
1. taking lung tissue in (), after paraformaldehyde is fixing, routine paraffin wax embeds, 5 μm sections.
2. section is conventional dewaxes with dimethylbenzene, through ethanol at different levels to washing: dimethylbenzene (I) 5min → dimethylbenzene (II) 5min → Ethanol 1min → 80% ethanol 1min → 75% ethanol the 1min of 100% ethanol 2min → 95% → distillation washing 2min.(each two Secondary, then water is blotted)
3. haematoxylin dyeing 5min, tap water rinses.(blotting water)
4. acidic alcohol differentiation 30s(carry slotting several under).
5. tap water soaks 15min or warm water (about 50 DEG C) 5min.(blotting water)
6. put Yihong liquid 2min.
7. conventional dehydration, transparent, mounting: 95% ethanol (I) min → 95% ethanol (II) 1min → 100% ethanol (I) 1min → 100% ethanol (II) 1min → dimethylbenzene (I) 1min → dimethylbenzene (II) 1min → resinene sealing.
The most smooth Microscopic observation also does pathological evaluation.
(3) chemical rejects mouse lung macrophage, to detect macrophage effect in CNT antitumor
1. inoculated tumour cell
Six week old male C57BL/6 mice 36, body weight 18-22g, test first day, every mice equal tail vein injection Lewis Lung carcinoma cell 1 × 105Individual.
2. packet
Postvaccinal mice is randomly divided into 3 groups (model control group, CNT group, CNT+macrophage rejecting groups), Often group 12.
3. packet transaction
1. model control group: after inoculating 24 hours, tracheal instillation 80 μ l carboxymethylcellulose sodium solution；
2. CNT group: after inoculating 24 hours, tracheal instillation 80 μ l functionalized multi-wall carbonnanotubes (4 mg/kg)；
3. CNT+macrophage rejecting group: after inoculating 24 hours, tracheal instillation 80 μ l functionalized multi-wall carbonnanotubes (4 Mg/kg), tracheal instillation clodronate liposome (6 mg/kg, 3 days/time) simultaneously
4. observe mice general survival condition every day and measure body weight.
5. take lung tissue, observe Lung metastases and count.
Injection tumor cell after 14 days, put to death mice, take lung tissue, observe and add up Lung metastases and count.
(4) result
1. functionalized multi-wall carbonnanotubes suppression neoplasm metastasis
1) Lung metastases is counted
Dissect mice, take out lung tissue, observe and add up Lung metastases and count.Result shows, functionalized multi-wall carbonnanotubes is (at figure Middle abbreviation CNT) dose-dependent inhibition Lewis lung cancer Lung metastases, make Lung metastases count during high dose and reduce by 47.2 %, such as Fig. 1. Meanwhile, functionalized multi-wall carbonnanotubes significance suppression melanoma Lung metastases, make Lung metastases count and decline 46.6 %, such as Fig. 2.
2) hematoxylin-eosin staining
Dissect mice, take out lung tissue, paraffin embedding, carry out hematoxylin-eosin (HE) dyeing.Result shows, Lewis lung cancer mould In type, model group lung tissue cell has the typical characteristic of low differentiation non-small cell carcinoma, as core have heteromorphism, nuclear hyperchromatism and High nucleocytoplasmic ratio etc., predominantly tumor cell；The cell mesh in low dosage CNT group with tumor cell feature has reduction to become Gesture, it is seen that part normal alveolar architecture；In high dose CNT group, major part is normal alveolar tissue, and tumor cell is dispersed in point Cloth, such as Fig. 3 A.Similar to Lewis lung cancer model result, in B16F10 melanoma tumor model, the how deep dye of model group lung tissue, carefully Karyon is big and irregular, major part tumor cell；Common normal alveolar architecture in CNT group, tumor cell greatly reduces, Such as Fig. 3 B.
2. functionalized multi-wall carbonnanotubes does not affect general animal survival state and body weight
Mouse survival is in good condition, does not occurs animal dead during experiment.The diet of different group mices and active state are without bright Significant difference is different.Result shows, in Lewis lung cancer model (Fig. 4 A) and B16F10 melanoma tumor model (Fig. 4 B), and each treated animal body Weight average there was no significant difference.
3. chemical is rejected mouse lung macrophage and is reversed CNT antitumor action
Dissect mice, take out lung tissue, observe and add up Lung metastases and count.Result shows, in Lewis lung cancer model, receives with carbon Mitron group is compared, and the Lung metastases of CNT+macrophage rejecting group is counted increases by 95.7 %, and i.e. rejecting mouse lung is huge bites carefully Born of the same parents reverse the inhibiting effect on tumor metastasis of CNT, such as Fig. 5.
(5) conclusion
Above-mentioned test result indicate that, functionalized multi-wall carbonnanotubes significance suppression neoplasm metastasis, and macrophage is sent out wherein Wave important effect.
The detection on tumor-associated macrophages polarization impact of [embodiment 2] functionalized multi-wall carbonnanotubes
(1) immunofluorescence dyeing
1. taking Lewis lung cancer animal pattern lung tissue in (), after paraformaldehyde is fixing, routine paraffin wax embeds, 5 μm sections.
2. paraffin section is immersed in antigen retrieval buffer (10 mM Tris, 0.5 mM EGTA aqueous solution, pH 9.0), microwave oven 500 W heats 20 minutes.
3. paraffin section is hatched in the PBS containing 5% bovine serum albumin (BSA).
4. one resists 4 ° of C overnight incubation.
5.PBS washs 3 times, and two anti-room temperature lucifuges hatch 1 hour.
6. PBS washs 3 times, drips DAPI dye liquor, and room temperature lucifuge hatches 10min.
7. PBS washs 3 times, with anti-fluorescent quenching mountant mounting after section slightly drying.
8. use microscope digital camera (DP80;OLYMPUSA, Japan) microscopy takes pictures.
(2) polymerase chain reaction
1. take Lewis lung cancer animal pattern lung tissue in (), use Trizol test kit to extract total serum IgE.
2. use UV detector detection RNA sample OD260 and OD280, detection total serum IgE purity and integrity.
3. use Reverse Transcriptase kit to extract and mRNA reverse transcription is obtained cDNA.
4. configure following reaction system: 2 μ l cDNA templates, 2 μ l primers, 12.5 μ l SYBR solution and 8.5 μ l are super Pure water.
5. use Applied Biosystems 7500 Real Time PCR System to expand.
6. use 2 Δ Δ Ct methods to carry out statistical analysis.
(3) result
1. immunofluorescence dyeing
Dissect mice, take lung tissue, after paraffin embedding, carry out immunofluorescence dyeing.Result such as Fig. 6, F4/80, iNOS and CD206 It is macrophage, M1 type macrophage and the phenotypic marker of M2 type macrophage respectively.Result shows, compared with model group, The macrophage ratio expressing iNOS in CNT group dramatically increases, and the macrophage ratio expressing CD206 significantly reduces.
2. polymerase chain reaction
Dissect mice, take lung tissue, use real-time quantitative PCR method detection lung tissue macrophage function state.Result such as Fig. 7, Functionalized multi-wall carbonnanotubes dose dependent increases the gene expression of M1 type macrophage phenotype mark iNOS, high dose carbon In nanotube group, iNOS gene expression increases nearly 4 times；Meanwhile, functionalized multi-wall carbonnanotubes dose-dependent inhibition M2 type is huge bites The gene expression of Cytophenotypic markers thing CD206, in high dose CNT group, CD206 gene expression reduces by 47.1 %.
(4) conclusion
Above-mentioned test result indicate that, functionalized multi-wall carbonnanotubes significance promotes that tumor-associated macrophages is reprogrammed by M2 type For M1 type, suppress neoplasm metastasis.
The result of comprehensive above experiment, conclusion, it is believed that functionalized multi-wall carbonnanotubes be treatment neoplasm metastasis and Reprogramming tumor-associated macrophages is the active drug of M1 type.

Claims (5)

1. functionalized multi-wall carbonnanotubes application in preparing medicine for anti transfer of tumor.
Application the most according to claim 1, it is characterised in that described functionalized multi-wall carbonnanotubes is external diameter 20-30 Nm, long 500 nm, the purity nano material higher than 98 %.
Application the most according to claim 1, it is characterised in that described tumor is pulmonary carcinoma.
Application the most according to claim 1, it is characterised in that described tumor is melanoma.
Application the most according to claim 1, it is characterised in that described anti metastasis is to be received by carboxylated many walls carbon Mitron promotes that tumor-associated macrophages is M1 type by M2 reprogramming and realizes.
CN201610651752.3A 2016-08-10 2016-08-10 Functionalized multi-wall carbonnanotubes application in preparing medicine for anti transfer of tumor Pending CN106265735A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610651752.3A CN106265735A (en) 2016-08-10 2016-08-10 Functionalized multi-wall carbonnanotubes application in preparing medicine for anti transfer of tumor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610651752.3A CN106265735A (en) 2016-08-10 2016-08-10 Functionalized multi-wall carbonnanotubes application in preparing medicine for anti transfer of tumor

Publications (1)

Publication Number Publication Date
CN106265735A true CN106265735A (en) 2017-01-04

Family

ID=57668051

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610651752.3A Pending CN106265735A (en) 2016-08-10 2016-08-10 Functionalized multi-wall carbonnanotubes application in preparing medicine for anti transfer of tumor

Country Status (1)

Country Link
CN (1) CN106265735A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109679914A (en) * 2019-01-16 2019-04-26 浙江省医学科学院 A kind of strain of human mesothelial cell vicious transformation and its application of multi-walled carbon nanotube induction

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101239187A (en) * 2007-02-09 2008-08-13 复旦大学附属华山医院 Functionalization carbon nano-tube for tumor lympha targeted therapeutic carrier

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101239187A (en) * 2007-02-09 2008-08-13 复旦大学附属华山医院 Functionalization carbon nano-tube for tumor lympha targeted therapeutic carrier

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
FUMIN JIA，等: "Preparation, characterization and fluorescent imaging of multi-walled carbon nanotube–porphyrin conjugate", 《J. MATER. CHEM.》 *
GRAHAM, ELIZABETH G.，等: "Multi-Walled Carbon Nanotubes Inhibit Breast Cancer Cell Migration", 《JOURNAL OF BIOMEDICAL NANOTECHNOLOGY》 *
MAN YANG，等: "Multiwalled Carbon Nanotubes Interact with Macrophages and Influence Tumor Progression and Metastasis", 《THERANOSTICS》 *
WEN LIU，等: "Aggregation and stabilization o f multiwalled carbon nanotubes in aqueous suspensions:in fluences of carboxymethyl cellulose, starch and humic acid", 《RSC ADV.》 *
李晓明，等: "《纳米颗粒与管状材料的生物安全性与毒性》", 31 July 2014, 知识产权出版社 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109679914A (en) * 2019-01-16 2019-04-26 浙江省医学科学院 A kind of strain of human mesothelial cell vicious transformation and its application of multi-walled carbon nanotube induction
CN109679914B (en) * 2019-01-16 2020-12-15 浙江省医学科学院 Human pleural mesothelial cell malignant transformation strain induced by multi-walled carbon nanotubes and application thereof

Similar Documents

Publication Publication Date Title
Yi et al. Imaging‐guided combined photothermal and radiotherapy to treat subcutaneous and metastatic tumors using iodine‐131‐doped copper sulfide nanoparticles
He et al. Tumor‐Penetrating Nanotherapeutics Loading a Near‐Infrared Probe Inhibit Growth and Metastasis of Breast Cancer
Roussakow The history of hyperthermia rise and decline
Liu et al. Metal-organic framework-mediated multifunctional nanoparticles for combined chemo-photothermal therapy and enhanced immunotherapy against colorectal cancer
CN112168963B (en) Nanometer photothermal therapeutic medicine and preparation method thereof
CN105797157B (en) A kind of preparation method and application of porous nucleocapsid bimetallic organic frame nano drug-carrying body
CN109091674B (en) Multifunctional drug carrier and preparation method and application thereof
Chi et al. Biomimetic Nanocomposites Camouflaged with Hybrid Cell Membranes for Accurate Therapy of Early‐Stage Glioma
CN109549954B (en) Phosphorus-based material preparation and preparation method and application thereof
Jia et al. Multi-functional self-assembly nanoparticles originating from small molecule natural product for oral insulin delivery through modulating tight junctions
CN106581674A (en) Multifunctional nano drug-loaded system and preparation method thereof
Jin et al. Indocyanine green-parthenolide thermosensitive liposome combination treatment for triple-negative breast cancer
CN109316485A (en) A kind of antitumor application thereof of covalent organic frame material
Yang et al. Polydopamine‐Engineered Theranostic Nanoscouts Enabling Intracellular HSP90 mRNAs Fluorescence Detection for Imaging‐Guided Chemo–Photothermal Therapy
Ataollahi et al. Fabrication and investigation potential effect of lentinan and docetaxel nanofibers for synergistic treatment of breast cancer in vitro
Xia et al. Copper nanocrystalline-doped folic acid-based super carbon dots for an enhanced antitumor effect in response to tumor microenvironment stimuli
CN105194679A (en) Preparation method and application of titanium dioxide-graphene oxide composite material modified by hyaluronic acid of antitumor drug nanometer layer
WO2016015172A1 (en) Tumor treatment method for blocking tumor vasculature by means of nanomaterial and external radiation source
CN107582691A (en) Compound phellodendron bark liquid paint is gargled gel
Tang et al. Cancer cell membrane biomimetic mesoporous nanozyme system with efficient ROS generation for antitumor chemoresistance
CN106606783B (en) A kind of targeting is passed altogether to be released the drug of photosensitizer and chemotherapeutics and passs release system
CN106265735A (en) Functionalized multi-wall carbonnanotubes application in preparing medicine for anti transfer of tumor
Zhao et al. Nanotechnology-enabled M2 macrophage polarization and ferroptosis inhibition for targeted inflammatory bowel disease treatment
CN109157531B (en) Porous bismuth nano-microsphere and preparation method and application thereof
Wang et al. Tumor microenvironment activated multifunctional nanoparticles for precisely controlled tumor photothermal and photodynamic therapy

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20170104

RJ01 Rejection of invention patent application after publication