CN106264776B - Fatty chylosis method - Google Patents
Fatty chylosis method Download PDFInfo
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- CN106264776B CN106264776B CN201610942264.8A CN201610942264A CN106264776B CN 106264776 B CN106264776 B CN 106264776B CN 201610942264 A CN201610942264 A CN 201610942264A CN 106264776 B CN106264776 B CN 106264776B
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- lipochondrion
- segmentation
- fat
- chylosis
- dispenser
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/0059—Cosmetic or alloplastic implants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M31/00—Devices for introducing or retaining media, e.g. remedies, in cavities of the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/178—Syringes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2240/00—Manufacturing or designing of prostheses classified in groups A61F2/00 - A61F2/26 or A61F2/82 or A61F9/00 or A61F11/00 or subgroups thereof
- A61F2240/001—Designing or manufacturing processes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2202/00—Special media to be introduced, removed or treated
- A61M2202/09—Body tissue
Abstract
The present invention relates to a kind of fatty chylosis methods, the method includes being purified the lipochondrion extracted, chylosis, obtain chyle sample fat, during chylosis by the way of dividing step by step, while strictly controlling the grain size and number divided step by step.Chylosis method can realize that skin is relatively thin or the fat transfer of sensitive part in the present invention, skin is smooth after transplanting, wound is small, resume speed is fast, is suitable for improving skin quality, improves the transplanting of the unstructuredness such as microgroove, and in combination with the common improvement for completing skin quality of structural fatty transplanting.
Description
Technical field
The present invention relates to lipoplasty field more particularly to a kind of fatty chylosis methods for fine structure filling.
Background technology
Skin aging is the skin aging phenomenon as caused by natural cause or non-natural factor.Skin histology day after people's birth
It reaches increasingly, function is gradually active, will start to degenerate when reaching certain age, and this degeneration is often unconsciously slow in people
Slow-motion row.The phenomenon that skin aging, is mainly manifested in skin histology decline.The thickness of skin has obviously with the increase at age
Change.It is most thick when the epidermis of people 20 years old, it is later gradually thinning, to senescence phase stratum granulosum can atrophy to disappearing, prickle cell's life cycle
Shorten.Corium is most thick at 30 years old, and later gradual change is thin and with atrophy.Subcutaneous fat reduce, and due to moisture, elastic fibers and
Collagenous fibres change and gradually lose skin elasticity and tension, and cutis laxa is further caused to be generated with wrinkle.
Skin aging is the natural law of life, is irresistible, but people can be prolonged by skin nursing and health care
Slow skin aging.Compared with frequently with method be injection hyaluronic acid method.Hyaluronic acid is present in human skin in the form of gluey
Dermal tissue in, be responsible for storage moisture, increase skin volume, allow skin to seem full, plentiful, flexible, however, injection
Hyaluronic acid can be absorbed completely by body, it is therefore desirable to periodic injections, and its price is high, holds time short, thus by
Many faces are small.
Structural fatty transplanting is the important method of modern lipoplasty, is chiefly used in improving because disease, wound or aging are drawn
Structure after the soft tissue injury risen changes, and lipochondrion is transplanted to human body frequently with a diameter of 0.84-1.19mm syringe needles
Privileged site theoretically has the possibility for improving skin aging, and more permanent than the effect of hyaluronic acid.However, for skin
Relatively thin or sensitive part, such as eyelid, lip, hand fat transfer, local solids flux in corium deep layer inject treat
When, the complication such as rough and uneven in surface, sebocystoma sample lesion and calcium scoring are susceptible to, it is good to improve shortage for skin texture
Good effect.Therefore, to make up the deficiency of structural fatty transplanting, and the improvement for skin texture, using specific method,
Reduce transplant fat particle size, (fit structure fat transfer sometimes) with improve lipochondrion fill after effect, be into
Row fat transfer improves the significant process of skin aging.
Invention content
In order to solve the above-mentioned technical problem, present inventor has performed sharp studies, provide a kind of fatty chylosis method,
The method includes the lipochondrion extracted is purified, chylosis, obtain chyle sample fat, used during chylosis
The mode divided step by step strictly controls the grain size and number divided step by step, and the lipochondrion for treating chyle is strictly screened,
It is filled using the chyle sample fat, satisfied filling effect and skin texture improvement is can reach, to complete this hair
It is bright.
The purpose of the present invention is to provide a kind of fatty chylosis method, the lipochondrion that the method includes extracting
Purified, chylosis, obtain chyle sample fat, wherein during chylosis using to lipochondrion carry out it is descending according to
The partitioning scheme step by step of secondary segmentation.
In a preferred embodiment, the purifying includes the rinsing of lipochondrion and the removal of non-fat tissue,
It is 2~3 times that rinsing liquid, which uses 0 DEG C~4 DEG C physiological saline, rinsing times, rinses 0.5~1min every time.
In a preferred embodiment, after the lipochondrion rinsing, the separation of lipochondrion and rinsing liquid is carried out,
Any one of separate mode in filtering or centrifugation, preferably centrifuges, wherein centrifugal rotational speed be 1000r/min~
3000r/min, centrifugation time are 2min~3min.
In a preferred embodiment, it after lipochondrion and rinsing liquid being centrifuged, collects and is located at fat
Lipochondrion in the middle part of stratum granulosum carries out chylosis operation.There is 121 aperture of different segmentation holes using three kinds during chylosis
Dispenser carry out the segmentation of lipochondrion, 1 segmentation diaphragm 12 is equipped with inside three kinds of dispensers,
The aperture of three kinds of segmentation holes 121 is respectively 0.8~1.2mm, 0.6~0.8mm and 0.4~0.6mm,
The segmentation times for the dispenser that aperture with segmentation holes 121 is 0.8~1.2mm are 5~10 times,
The segmentation times for the dispenser that aperture with segmentation holes 121 is 0.6~0.8mm are 5~10 times,
The segmentation times for the dispenser that aperture with segmentation holes 121 is 0.4~0.6mm are 10~20 times.
According to a kind of fatty chylosis method provided by the invention, have the advantages that：
(1) chylosis method can realize that the reasonable segmentation of lipochondrion, bound fat implantation technique can be realized in the present invention
Skin is relatively thin or the fat transfer of sensitive part, such as eye circumference, mouth week, the non-fat structural transplanting region of hand, skin after transplanting
Skin is smooth, and wound is small, and resume speed is fast, can significantly improve skin elasticity, is suitable for improving the non-of skin quality, improvement microgroove etc.
Structural transplanting；
(2) present invention accurately defines the concrete operations of chylosis method, has to chyle sample fat transfer and refers to
Lead meaning.
Description of the drawings
Fig. 1 shows the dispenser structural schematic diagram in a kind of preferred embodiment of the present invention；
Fig. 2 shows the segmentation diaphragm structure schematic diagrames in a kind of preferred embodiment of the present invention；
Fig. 3 shows the structural schematic diagram of the extraction needle in a kind of preferred embodiment of the present invention.
Drawing reference numeral explanation：
11- split cylinders；
12- divides diaphragm；
121- segmentation holes；
13- interfaces；
21- needle points；
22- needle tubings；
221- pin holes；
222- bending points；
23- connecting seats.
Specific implementation mode
Present invention will now be described in detail, and the features and advantages of the invention will become more with these explanations
It is clear, clear.
Dedicated word " exemplary " means " being used as example, embodiment or illustrative " herein.Here as " exemplary "
Illustrated any embodiment should not necessarily be construed as preferred or advantageous over other embodiments.Although each of embodiment is shown in the accompanying drawings
In terms of kind, but unless otherwise indicated, it is not necessary to attached drawing drawn to scale.
It is pure the method includes carrying out the lipochondrion extracted according to fatty chylosis method provided by the invention
Change, chylosis, obtains chyle sample fat.
In a preferred embodiment, the purifying includes the rinsing of lipochondrion and the removal of non-fat tissue.
Rinsing liquid uses physiological saline, implements the rinsing of lipochondrion.After the lipochondrion of suction is collected, immediately with life
Saline rinse is managed, the blood taken out of on lipochondrion is washed away.The osmotic pressure of physiological saline is identical as human plasma, can avoid
Adipocyte ruptures, and after swelling anaesthesia, adipocyte osmotic pressure changes (becoming smaller), and physiological saline osmotic pressure is thin higher than fat
Born of the same parents are conducive to the discharge of excess moisture in adipocyte while cleaning.
Rinsing is low-temperature normal saline with physiological saline, and temperature is 0 DEG C~4 DEG C.At low temperature, the substance in adipocyte
Metabolism slows down, and oxygen demand reduces, and can keep the activity of adipocyte, increases the survival rate of adipocyte in rinse cycle.
The number of physiological saline rinsing is 2~3 times, rinses 0.5~1min every time, and fat is completed within the extremely short time
The rinsing of grain.
The removal of impurities of lipochondrion is carried out during rinsing, non-fat tissue (predominantly fiber group is completed in manual operations
Knit) removal.Since lipochondrion extracts from patient thigh inside or lower abdominal region, fibr tissue is less, therefore, can float
Removal of impurities work is completed in the short period washed, and saves the operating time.The patient is the natural person for carrying out fat transfer operation.
In a preferred embodiment, it after being rinsed with physiological saline, stands, collects the fat on physiological saline upper layer
Grain, obtains lipochondrion suspension, then carries out the separation of solid and liquid of lipochondrion suspension.Separate mode is selected from filtering or centrifugation
Any one mode in separation, preferably centrifuges.During centrifugation, under the influence of centrifugal force, in adipocyte
Due to swelling anaesthesia, existing more moisture further flows out, and after the completion of centrifugation, the size of adipocyte is about contracted to carry
The 1/2 of size when taking.
Centrifugal process is to centrifuge 2min~3min at rotating speed 1000r/min~3000r/min.Centrifugal rotational speed and centrifugation
There is a stringenter restriction in time, and centrifugal rotational speed is excessive or the time the long more is easy to cause the rupture of adipocyte, but centrifugation rate
It is small, the cell fragment brought when lipochondrion extraction can not be removed to greatest extent.It is fatty after centrifugation in this rotating speed and in the time
The purity of cell significantly improves before relatively centrifuging.
After the completion of centrifugation, lipochondrion suspension layering, upper layer is oil layer, and lower layer is physiology brine layer, and centre is fat
Fat stratum granulosum.It takes the lipochondrion in the middle part of lipochondrion layer and carries out chylosis processing, it is preferable that the lipochondrion of selection
1/3 of amount less than lipochondrion total amount.Lipochondrion layer top easily attaches grease close to oil layer, the lipochondrion on top, and
There may be remaining fibr tissue, impurity present in two parts can influence the implantation of fat for the lower part of lipochondrion layer
Amount, influences filling effect.The 1/3 lipochondrion epigranular in the middle part of lipochondrion layer, fibr tissue is few, purity higher,
For the optimal selection of transplant fat.
In a preferred embodiment, chylosis device includes fatty dispenser and injector.As shown in Figure 1, institute
It includes split cylinder 11 and the segmentation diaphragm 12 in split cylinder 11 to state dispenser.The split cylinder 11 is holding for both ends open
Lipochondrion by hollow cylinder, be connected with injector by the interface 13 set on two open ends, i.e. dispenser cooperation
Use two injectors.There is screw thread in 13 outer surface of the interface, for fixing injector.
As depicted in figs. 1 and 2, the segmentation diaphragm 12 is connected to 11 middle part of split cylinder, and dividing has on diaphragm 12 equipped with circle
Shape segmentation holes 121, large-sized lipochondrion are divided into small particle lipochondrion by segmentation holes 121.Divide film on dispenser
The number of piece 12 is 1~3, preferably 1.Divide the number 1~3 of segmentation holes 121 on diaphragm 12, segmentation holes 121
When number is 1, segmentation holes 121 is located at the center of segmentation diaphragm 12；When the number of segmentation holes 121 is 2, two segmentation holes 121 are side by side
It is arranged on the center line of segmentation diaphragm 12；When the number of segmentation holes 121 is 3, three segmentation holes 121 are using center line as symmetry axis
Finished product shape is arranged.The shape in circular dividing hole 121 meets the form of adipocyte, can utmostly keep adipocyte thin
The integrality of after birth.
The injector is located at dispenser both ends, for drawing lipochondrion to be split, and makes it through dispenser
Segmentation holes 121 completes cutting procedure.Injector includes injecting pipe and positioned at the piston rod injected in pipe, by applying to piston rod
Pressure pushes the movement of lipochondrion.The injector can be injector for medical purpose.
By the way of dividing step by step, the segmentation step by step refers to carrying out lipochondrion by big for the chylosis of lipochondrion
To the small mode divided successively, the side for dividing lipochondrion successively using the dispenser of segmentation holes aperture from large to small is referred specifically to
Method rather than it is general just use lipochondrion is disposably divided to the mode of required particle size.When dividing step by step, segmentation holes
121 aperture from large to small, makes the grain size of lipochondrion there is the process that gradually changes, to the pressure phase of lipochondrion when segmentation
There is very big reduction compared with disposable segmentation, avoids adipocyte gross distortion or rupture when squeezing segmentation.
In a preferred embodiment, point that there is 121 aperture of different segmentation holes using three kinds during chylosis
Cutter carries out the segmentation of lipochondrion, and 1 segmentation diaphragm 12, the hole of three kinds of segmentation holes 121 are equipped in three kinds of dispensers
Diameter is respectively 0.8~1.2mm, 0.6~0.8mm and 0.4~0.6mm, point that the aperture with segmentation holes 121 is 0.8~1.2mm
The segmentation times of cutter are 5~10 times, and the segmentation times for the dispenser that the aperture with segmentation holes 121 is 0.6~0.8mm are 5
~10 times, the segmentation times for the dispenser that the aperture with segmentation holes 121 is 0.4~0.6mm are 10~20 times, are obtained faint yellow
Chyle sample fat, wherein the grain size of lipochondrion is less than 0.5mm.
In further preferred embodiment, using the dispenser in three kinds of segmentation apertures, 121 pore size of segmentation holes is
1.0mm, 0.8mm and 0.5mm.When carrying out lipochondrion segmentation, use 121 aperture of segmentation holes for the dispenser of 1.0mm, fat
The segmentation times of particle are 5 times；Then use 121 aperture of segmentation holes for the dispenser of 0.8mm, the segmentation times of lipochondrion are
10 times；It is the dispenser of 0.5mm finally to use 121 aperture of segmentation holes, and the segmentation times of lipochondrion are 15 times.
Chyle sample fat for relatively thin or sensitive part skin effects improvement mainly by chyle sample fat for
The stimulation of differentiation and the regeneration of body fat cell hypertrophy, and filling effect caused by the lipochondrion of implantation obtain
, thus segmentation times of lipochondrion, in particular by smallest partition aperture dispenser when segmentation times, to fat
Effect after being implanted into the patient is most important, is mainly manifested in：When 121 pore size of smallest partition hole is 0.4~0.6mm,
Segmentation times are less than 10 times, and lipochondrion movement in injector is not smooth, and lipochondrion is not uneven, fine and smooth, at this time to suffering from
Person, which is injected, not only increases injection difficulty, and the more difficult assurance of the flatness of fill area, influences postoperative effect after injection；Segmentation time
Number is more than 20 times, and lipochondrion is mobile smooth in injector, and lipochondrion is more uniform, however repeated segmentation causes lipochondrion
Small and density is low, big in the mobility of fill area, is absorbed or excluded in vitro by body tissue quickly after being implanted into the patient, right
The stimulation of adipocyte is limited in body.In segmentation times 10~20 times, the color of lipochondrion is become from buff
Faint yellow, lipochondrion is uniform, and grain density is moderate, is suitable for transplanting.
In the present invention, the fatty chylosis method further includes selecting the privileged site extraction lipochondrion of patient, with
And chylosis fat is injected to position to be filled.
In a preferred embodiment, it carries out fat-extraction operation consent and Haemodynamics, selection is carried out to patient
The privileged site of patient extracts lipochondrion.
Lipochondrion extracts from the inside of patient thigh or lower abdominal region, not only due to the fat at these positions is more, more
It is important that the fibr tissue at these positions is less, to reduce the difficulty of post-processing, and lipochondrion plumpness is high, is easy into
It is living.
Swelling anaesthesia is implemented to patient before fat-extraction, will be largely filled into containing lidocaine and adrenergic anesthesia liquid
Subcutaneously, adrenergic topical application makes local vasoconstriction, drug absorption slow down, and extends anesthesia timeliness；Fat and benefit
Cacaine has compatibility, can delay its absorption；A large amount of injection Tumescent fluids simultaneously, make subcutaneous tissue and its structure generate oedema, part
The tension of tissue increases, and directly can oppress capillary and reduce the absorption of drug, thus can realize local anaesthesia analgesic, hemostasis
And the effect of tissue separation.Since adipocyte gap increases, during fat-extraction, large area adipocyte is not easily caused
Damage.
In a preferred embodiment, the anesthesia liquid using physiological saline be solvent, wherein lidocaine it is dense
It is 0.025 %~0.1 (w/v) (w/v) % to spend, adrenergic a concentration of 0.5 × 10-7(w/v) %~1 × 10-7(w/v) %.
In a preferred embodiment, the lipochondrion extracted extracts to obtain by using extraction needle.Such as Fig. 3 institutes
Show, the extraction needle includes needle point 21, needle tubing 22 and connecting seat 23 connected in sequence, 21 not trepanning of the needle point, for guiding
The movement of needle tubing 22；The needle tubing 22 is used for transmission the lipochondrion extracted, and the one end being connected with needle point 21 is equipped with along spiral shell
Revolve multiple pin holes 221 of shape distribution；The connecting seat 23 is for connecting needle tubing 22 and negative pressure device.The negative pressure device utilizes negative
Pressure detaches lipochondrion in vivo via extraction needle, for professional medical equipment, such as syringe.
The pin hole of existing fat-extraction needle is arranged in triangle disposition, and such arrangement mode, which easily causes, takes that fat is uneven, takes
Fat region is uneven and connecting liposuction uneven the problems such as resulting in blockage in needle tubing.In the present invention, spiral distribution
Pin hole 221 make extraction needle form column in fat deposit to take fat, column takes fat utmostly extraction fat in unit area
Cell takes fat coherent uniform, avoids and extract back and forth, and 22 blockage problem of needle tubing in art will not occur, has tissue damage
Hinder the heavy few feature of small, pinprick color, at the same reduce anesthesia duration and Anesthesia medicine, reduce the accumulation of narcotics in vivo with
Metabolism, increases the safety of operation.
In further preferred embodiment, the pin hole 221 is round or oval, due to ripe adipocyte
It is round or oval, the shape of pin hole 221 meets the form of adipocyte, can utmostly keep adipocyte cell membrane
Integrality.According to different length, the extraction needle of caliber, the number of pin hole 221 is 3~5, preferably 3, utmostly
While ensureing fat-extraction amount, the suction strength in extraction needle can be preferably kept.
In embodiment still more preferably, according to small apart from 21 nearer end negative pressure of needle point, farther out apart from needle point 21
The feature for holding negative pressure big, in the hand of spiral along needle point 21 to connecting seat 23, the descending distribution in aperture of pin hole 221, this
Kind aperture design ensure that pressure uniformity of the fat in extraction process, protect the form of adipocyte consistent.It is preferred that
Ground extracts the needle tubing 22 of needle close to the V-shaped bending in 23 one end of connecting seat, and bending angle is 120 °~135 °, it is highly preferred that bending
222 distances away from connecting seat 23 of point are the 1/5~1/7 of 22 length of needle tubing.Fat position, such as buttocks, bending extraction are taken specific
Needle is used compared to the unbending extraction needle person of being more convenient to operate, and can be easily accomplished 3 faces and be taken fat for totally 360 °, and human body life is met
Manage curvature.The bending point 222 refers to the point that needle tubing 22 starts bending.
When chyle sample fat is implanted into patient's corium deep layer, using multiple spot, it is multi-level, inject on a small quantity by the way of, ensuring fat
The problems such as uneven, sebocystoma sample change and lipochondrion both central necrotic are avoided while fat cell survival rate.
Chyle sample fat injection is equivalent to autograft process, meets medical standard, can be used for improving skin
Skin quality amount, the rejuvenation field for improving the transplanting of the unstructuredness such as microgroove are likely to become the mark of unstructuredness transplanting rejuvenation in the future
It is accurate.In fat is performed the operation, the normal fit structure fat transfer (granular cell of chyle sample fat transfer (unstructuredness fat transfer)
Transplanting) it is used in conjunction with, such as when improving deeper wrinkle, it need to be implanted into larger particles cell in deep skin, coordinate the breast of shallow-layer
Rotten sample fat, it is common to complete.
Embodiment
Embodiment 1
(1) preparation method of chyle sample fat
1, patient thigh's inside region is selected, suction lipectomy, preoperative low dose low concentration injection are carried out with special fat suction needle
(0.05 (w/v) % lidocaines+10-7(w/v) % adrenaline) carry out Haemodynamics；
2, it after collecting the lipochondrion of suction, is rinsed 2 times with 1min/ times with 4 DEG C of physiological saline, stands 0.5min, receive
Collect upper-layer fat particle, is separated by solid-liquid separation, upper part suspended oil fat is sucked out, lower end moisture with 3000r/min centrifugations 2min
Discharge；
3,1/3 lipochondrion is used with 1mL spiral syringes extraction lipochondrion in the middle part of selection lipochondrion layer
The dispenser in the tri- kinds of segmentation apertures 1.0mm, 0.8mm and 0.5mm carries out lipochondrion segmentation, wherein divides film in each dispenser
Piece number is 1, and segmentation holes number is followed successively by 3,2 and 1.The dispenser segmentation 5 in 1.0mm segmentations aperture is used first
It is secondary；Then the dispenser in 0.8mm segmentations aperture is used to divide 10 times；The dispenser for the use of segmentation aperture being finally 0.5mm is divided
15 times, obtain chyle sample fat.
(2) injection effect of chyle sample fat
Patient：Female, 10 people, 47-55 Sui, forehead line and crow's feet are apparent (aging eye circumference microgroove, eye circumference pigmentation),
The fat filling of crumple zone is carried out using fatty chyle technology fit structure fat transfer technology.
By the chyle fat of extraction, corium deep layer injection is carried out with 27G syringe needles (internal diameter 0.21mm, outer diameter 0.41mm), directly
Until xanthochromia variation occurs for skin color.This discoloration would generally a few hours disappearance after injection.
Effect tracking in 6 months is carried out after operation, after chyle sample fat and structural fatty are transplanted, forehead line improves bright
Aobvious, eye circumference microgroove disappears, and adipocyte transplantation effect satisfaction, pigmentation improves the elastic increase of bright and local skin.Observation hair
Existing, chyle sample fat has apparent tender skin effect, concrete outcome to be shown in Table 1 patient facial region.
Table 1 is preoperative, postoperative outcome compares
Note：1, forehead line or crow's feet obvious degree：1 grade：There is no visible scrimp；Only see continuous skin streakline；2
Grade：Fold lines are as it can be seen that be in slight indentation, facial scrimp is tiny；3 grades：Deep gauffer, facial scrimp is clear, in general feelings
Scrimp is visible under condition；4 grades：Deep gauffer；Facial scrimp is apparent；There is the visible scrimp less than 2mm when stretching, extension；5 grades：Profound wrinkle
, there are the high-visible scrimp of 2-4mm in pleat, when stretching, extension.
2, flatness (caused by operation)：1 grade：Without apparent raised or sunken；2 grades：Operative site has average 0.5mm convex
It rises；3 grades：Operative site has average 1mm protrusions；4 grades：Operative site, which has, is averagely more than 1-2mm protrusions；5 grades：Operative site has flat
It is all higher than 2mm protrusions.
3, eye circumference pigmentation：1 grade：Without apparent pigmentation；2 grades：It is shallower, slightly visible pigmentation；3 grades：It is brighter
Aobvious pigmentation；4 grades：Calm apparent, color is deep.
4, skin elasticity：1 grade：Elasticity is good；2 grades：It is elastic preferable；3 grades：Elastic unobvious；4 grades：Poor flexibility.
Embodiment 2
(1) preparation method of chyle sample fat
1, step 1 is the same as embodiment 1；
2, it after collecting the lipochondrion of suction, is rinsed 3 times with 0.5min/ times with 4 DEG C of physiological saline, stands 0.5min,
Upper-layer fat particle is collected, is separated by solid-liquid separation, upper part suspended oil fat is sucked out, lower end water with 2000r/min centrifugations 2min
Divide discharge；
3,1/3 lipochondrion is used with 1mL spiral syringes extraction lipochondrion in the middle part of selection lipochondrion layer
The dispenser in the tri- kinds of segmentation apertures 1.0mm, 0.8mm and 0.5mm carries out lipochondrion segmentation, wherein divides film in each dispenser
Piece number is 1, and segmentation holes number is followed successively by 3,2 and 1.The dispenser segmentation 5 in 1.0mm segmentations aperture is used first
It is secondary；Then the dispenser in 0.8mm segmentations aperture is used to divide 10 times；The dispenser for the use of segmentation aperture being finally 0.5mm is divided
10 times, chyle sample fat is obtained, it is spare.
(2) injection effect of chyle sample fat
Patient：Female, 10 people, 52-61 Sui, neck line and corners of the mouth line are apparent, using fatty chyle technology fit structure fat
Implantation technique carries out the fat filling of crumple zone.
By the chyle fat of extraction, corium deep layer injection is carried out with 27G syringe needles, is until xanthochromia variation occurs for skin color
Only.
Effect tracking in 6 months is carried out after operation, after chyle sample fat and structural fatty are transplanted, neck line obviously changes
Kind, corners of the mouth line disappears and skin flat smooth, hence it is evident that and it improves the quality of patient skin and improves skin colour, concrete outcome
It is shown in Table 2.
Table 2 is preoperative, postoperative outcome compares
Note：Wrinkle degree, flatness and skin elasticity criterion are the same as table 1.
Embodiment 3
(1) preparation method of chyle sample fat
Step 1~3 are the same as embodiment 1.
(2) injection effect of chyle sample fat
Patient：Female, 10 people, 26-32 Sui, scar is apparent after caesarean birth, and scar area is carried out using fatty chyle technology
Fat filling.
By the chyle fat of extraction, corium deep layer injection is carried out with 27G syringe needles.
Effect tracking in 6 months is carried out after operation, after chyle sample fat transfer, scar area elasticity is obviously improved, color
Pool reduces with surrounding skin aberration, hence it is evident that improves the quality of patient's scar area skin, concrete outcome is shown in Table 3.
Table 3 is preoperative, postoperative outcome compares
Note：Flatness, skin elasticity and pigmentation criterion are the same as table 1.
Comparative example 1
(1) preparation method of chyle sample fat
1, step 1 is the same as embodiment 1；
2, it after collecting the lipochondrion of suction, is rinsed 2 times with 1min/ times with room temperature physiological saline, stands 0.5min, receive
Collect upper-layer fat particle, is separated by solid-liquid separation with 600r/min centrifugations 2min, upper part suspended oil fat is sucked out, lower end moisture row
Go out；
3, lipochondrion layer whole lipochondrion is selected, lipochondrion is extracted with 1mL spiral syringes, directly uses
The dispenser that 0.5mm divides aperture carries out lipochondrion segmentation, and the number that diaphragm is divided in dispenser is 1, segmentation holes number
It is followed successively by 3.Dispenser is divided 30 times, and chyle sample fat is obtained.During chylosis, fat is increased by the resistance of segmentation holes
Add, adipocyte crush injury may be caused big, the process for making chyle sample fat is easy to happen interim card.
(2) injection effect of chyle sample fat
Patient：Female, 10 people, 42-50 Sui, forehead line and crow's feet are apparent, using fatty chyle technology fit structure fat
Fat implantation technique carries out the fat filling of crumple zone.
By the chyle fat of extraction, corium deep layer injection is carried out with 27G syringe needles, is until xanthochromia variation occurs for skin color
Only.
Effect tracking in 6 months is carried out after operation, after chyle sample fat and structural fatty are transplanted, forehead line and fish tail
Line is still more apparent, not be improved significantly, for the quality and skin colour effect of skin without large change, concrete outcome is shown in Table 4 institutes
Show.
Table 4 is preoperative, postoperative outcome compares
Note：Wrinkle degree, flatness, skin elasticity and pigmentation criterion are the same as table 1.
Comparative example 2
(1) preparation method of chyle sample fat
1, step 1 is the same as embodiment 1；
2, it after collecting the lipochondrion of suction, is rinsed 2 times with 1min/ times with 4 DEG C of physiological saline, stands 0.5min, receive
Collect upper-layer fat particle, is separated by solid-liquid separation, upper part suspended oil fat is sucked out, lower end moisture with 3000r/min centrifugations 2min
Discharge；
3, select lipochondrion layer whole lipochondrion, with 1mL spiral syringes extract lipochondrion, using 1.0mm,
The dispenser in the tri- kinds of segmentation apertures 0.8mm and 0.5mm carries out lipochondrion segmentation, wherein divides diaphragm number in each dispenser
It it is 1, segmentation holes number is followed successively by 3,2 and 1.The dispenser in 1.0mm segmentations aperture is used to divide first 5 times；Then
The dispenser for being divided aperture using 0.8mm is divided 5 times；The dispenser for the use of segmentation aperture being finally 0.5mm is divided 5 times, obtains
Chyle sample fat, it is spare.
(2) injection effect of chyle sample fat
Patient：Female, 10 people, 50-63 Sui, forehead line and crow's feet are apparent, using fatty chyle technology fit structure fat
Fat implantation technique carries out the fat filling of crumple zone.
By the chyle fat of extraction, corium deep layer injection is carried out with 27G syringe needles, is until xanthochromia variation occurs for skin color
Only.
Effect tracking in 6 months is carried out after operation, after chyle sample fat and structural fatty are transplanted, skin flatness is even
Out-of-flatness is sent out, skin elasticity improvement is limited, and concrete outcome is shown in Table 5.
Table 5 is preoperative, postoperative outcome compares
Note：Wrinkle degree, flatness, skin elasticity and pigmentation criterion are the same as table 1.
It is described the invention in detail above in association with specific implementation mode and/or exemplary example, but these are said
It is bright to be interpreted as limitation of the present invention.It will be appreciated by those skilled in the art that in the feelings without departing from spirit and scope of the invention
, can be with various equivalent substitutions, modifications or improvements are made to the technical scheme of the invention and its embodiments under condition, these each fall within this
In the range of invention.Scope of protection of the present invention is subject to the appended claims.
Claims (9)
1. a kind of fat chylosis method, which is characterized in that the method includes being purified the lipochondrion extracted, breast
Chylification obtains chyle sample fat,
Wherein, it is used during chylosis and the descending partitioning scheme step by step divided successively is carried out to lipochondrion；
The purifying includes the rinsing of lipochondrion and the removal of non-fat tissue, and rinsing liquid uses 0 DEG C~4 DEG C physiological saline；
After the lipochondrion rinsing, the separation of lipochondrion and rinsing liquid is carried out, separate mode is to centrifuge, centrifugal rotational speed
For 1000r/min~3000r/min, centrifugation time is 2min~3min；
Chylosis device during chylosis includes fatty dispenser and injector；
The segmentation of lipochondrion, three kinds of segmentations are carried out during chylosis using three kinds of dispensers with different segmentation holes apertures
It is equipped with 1 segmentation diaphragm (12) inside device, has equipped with circular dividing hole on segmentation diaphragm (12),
The aperture in three kinds of different circular dividing holes is respectively 0.8~1.2mm, 0.6~0.8mm and 0.4~0.6mm,
The segmentation times for the dispenser that aperture with segmentation holes is 0.8~1.2mm are 5~10 times,
The segmentation times for the dispenser that aperture with segmentation holes is 0.6~0.8mm are 5~10 times,
The segmentation times for the dispenser that aperture with segmentation holes is 0.4~0.6mm are 10~20 times；
The lipochondrion extracted extracts to obtain by using extraction needle, and the extraction needle includes needle point connected in sequence
(21), needle tubing (22) and connecting seat (23), the needle point (21) are used to guide the movement of needle tubing (22), the needle tubing (22) to be used for
The lipochondrion extracted is transmitted, the one end being connected with needle point (21) is equipped with multiple pin holes (221) along spiral distribution, institute
Connecting seat (23) is stated to be used to connect needle tubing (22) and negative pressure device,
In the hand of spiral along needle point (21) to connecting seat (23), the descending distribution in aperture of pin hole (221).
2. according to the method described in claim 1, it is characterized in that,
Rinsing times to lipochondrion are 2~3 times, rinse 0.5~1min every time.
3. according to the method described in claim 1, it is characterized in that, after lipochondrion and rinsing liquid are centrifuged, select
Chylosis operation is carried out with the lipochondrion in the middle part of lipochondrion layer.
4. according to the method described in claim 3, it is characterized in that, the lipochondrion amount selected is less than the 1/ of lipochondrion total amount
3。
5. method according to claim 1 to 4, which is characterized in that
The dispenser includes split cylinder (11) and the segmentation diaphragm (12) in split cylinder (11), and the split cylinder (11) is
Both ends open hold lipochondrion by hollow cylinder, be connected with injector by the interface (13) set on two open ends,
There is screw thread in interface (13) outer surface, and to fix injector, the segmentation diaphragm (12) is connected in the middle part of split cylinder (11), greatly
The lipochondrion of grain size is divided into small particle lipochondrion by segmentation holes；
The injector is located at dispenser both ends, for drawing lipochondrion to be split, and is run through the segmentation of dispenser
Hole is to implement chylosis process.
6. according to the method described in claim 1, it is characterized in that, lipochondrion extracts from the inside of patient thigh or lower abdomen area
Domain.
7. according to the method described in claim 1, it is characterized in that, the pin hole (221) of the extraction needle is round or oval.
8. according to the method described in claim 1, it is characterized in that, the needle tubing (22) of extraction needle is in close to connecting seat (23) one end
V-shaped bending, bending angle are 120 °~135 °.
9. according to the method described in claim 8, it is characterized in that, distance of the bending point (222) away from connecting seat (23) is needle tubing
(22) the 1/5~1/7 of length.
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| CN107962013B (en) * | 2017-12-28 | 2023-09-01 | 山西阳光中天医疗器械有限公司 | Automatic cleaning device for fat emulsion |
| CN110982784B (en) * | 2019-05-13 | 2023-12-29 | 杭州美天美医疗美容门诊部有限公司 | Production method and application of nano fat particles |
| CN111544650B (en) * | 2020-04-09 | 2021-07-13 | 中国人民解放军总医院第四医学中心 | Method and device for preparing super-emulsified erosive fat |
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| US6020196A (en) * | 1996-05-09 | 2000-02-01 | Baxter International Inc. | Devices for harvesting and homogenizing adipose tissue containing autologous endothelial cells |
| CN2273192Y (en) * | 1996-07-16 | 1998-01-28 | 戚可名 | Soxhlet's apparatus |
| FR2890976B1 (en) * | 2005-09-20 | 2007-12-14 | Univ La Reunion | KIT FOR PREPARING A COMPOSITION COMPRISING ADIPOCYTE CELLS AND METHOD FOR OBTAINING THE SAME |
| CN100529061C (en) * | 2005-10-21 | 2009-08-19 | 上海国睿生命科技有限公司 | Method for separating adipose cell in marrow and application therefor |
| CN200987808Y (en) * | 2006-04-27 | 2007-12-12 | 黄学峰 | Auto-fat transplantation adipocyte collecting screening syringe |
| CN101148657A (en) * | 2006-09-20 | 2008-03-26 | 北京大学第三医院 | Construction for tissue engineering cartilage modified by transforming growth factor beta2 gene |
| US8309342B2 (en) * | 2007-12-04 | 2012-11-13 | Ingeneron, Inc. | Apparatus and methods for cell isolation |
| CN201595869U (en) * | 2010-02-24 | 2010-10-06 | 魏邦敏 | Liposuction needle |
| IT1400069B1 (en) * | 2010-05-20 | 2013-05-17 | Tremolada | DEVICE AND METHOD FOR THE PREPARATION OF FABRIC, IN PARTICULAR ADIPOUS FABRIC FOR TRANSPLANTATION OBTAINED FROM ADIPOUS LOBULAR MATERIAL EXTRACTED THROUGH LIPOSUCTION |
| CN202740469U (en) * | 2012-05-24 | 2013-02-20 | 冯斌 | Flat-layer liposuction needle for high-definition progressive scanning liposuction |
| CN103961743A (en) * | 2013-01-31 | 2014-08-06 | 游英党 | Composite fat particle filler and preparation method thereof |
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