CN106260742B - Application of the antioxidant in abatement animal body in mycotoxin residual - Google Patents
Application of the antioxidant in abatement animal body in mycotoxin residual Download PDFInfo
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Abstract
The invention belongs to biological Control Technology fields, glutathione-S-transferase (UGT), glucuronyl transferase (SULT) or sulfotransferase (AANAT) in the gut of shrimp that do not contaminate can be activated by research discovery Quercetin, rutin or tea polyphenols, to promote the bioconversion of II phase enzyme, the activity of the contamination endogenous detoxication enzyme of gut of shrimp is induced by adding inducer in prawn feed to accelerate the metabolism of prawn vivotoxin, reduce or remove the residual of toxin, it is ensured that prawn food safety.
Description
Technical field
The present invention relates to biological Control Technology fields, and more particularly, to antioxidant, fungi is malicious in abatement animal body
Application in element residual.
Background technique
T-2, AF, DON and OTA are the mycotoxin for being widely present in nature, crops easy to pollute, to influence with agriculture
Aquatic feeds of the crop as raw material are the Common fungi toxin of strong carcinogenic, teratogenesis in aquatic feeds, toxicity, and compared with low-residual
Level can bring larger food safety risk.It can be accumulated by large aquaculture chain such as prawn, Tilapia mossambica, it is dynamic to endanger aquatic products
Object is even accumulated wherein, and then passes to people and animals by food chain, endangers human and livestock health, and therefore, control is raised with aquatic products is eliminated
The mycotoxin of material is extremely urgent.
Mycotoxin enters organism and carries out bioconversion, and bioconversion is the important ring that body disposes exogenous polyamines
Section is the main mechanism that body maintains stable state.Metabolizing enzymes (metabolic enzyme) play very important effect in this course,
Make the allogenic materials such as endotoxin excrete or be converted to other substances to excrete, reduces damage of the toxin to body.Biology
Conversion reaction mainly has phase Ⅰreaction (oxidation, reduction, hydrolysis) and phaseⅡreaction (association reaction).Wherein phaseⅡreaction is
Self a kind of physiology security mechanism that body copes with allogenic material, maintains homeostasis.Allogenic material structure is different, in life
The intracorporal II phase metabolic response type of object is also different.Glutathione S-transferase (Glutathione S-transferase,
GSTs), glucuronyl transferase (Uridine diphosphate glucuronyl transferase, UGT), sulfo group turn
Move enzyme (Sulfotransferase, SULT), aromatic amine N- acetyl transferase (Arylamine N-
Acetyltransferase, NATs) and transmethylase (Methyltransferase, MT) be main II phase enzyme.These
Enzyme can protect body from the infringement of the substances such as toxicity and some active materials, reduce the harm of toxin, convert it into it
His substance excretes, therefore also often they are referred to as II phase detoxication enzyme or II phase antioxidase.
The height of enzymatic activity directly affects reaction process, and high enzyme activity can make the bioconversion speed of metabolism substrate under normal circumstances
Degree enhancing, accelerates the accretion rate of toxin, to influence toxin to the effect size of body.Therefore, these key enzymes are induced
Gene expression induces its enzyme activity increase that can efficiently reduce the related disease that various foreign substances cause.Common inducer
Matter such as Quercetin, rutin, tea polyphenols and sulforaphane etc. can induce II phase detoxication enzyme, make its increased activity.Using induction/swash
Method living promotes enzyme content/vigor, and then accelerates the resolution of the allogenic materials such as mycotoxin, is conducive to mycotoxin in raw-food material
Control reduces food safety accident.
Summary of the invention
The technical problem to be solved by the present invention is to overcome drawbacks described above of the existing technology, antioxidant is provided and is being disappeared
Subtract the application in animal body in mycotoxin residual.
The purpose of the present invention is what is be achieved by the following technical programs:
Antioxidant improve detoxication enzyme detoxicating activity in application, the antioxidant be selected from Quercetin, rutin or
Tea polyphenols；The detoxication enzyme includes glutathione-S-transferase, aminopherase, glucuronyl transferase and sulfo group transfer
Enzyme.
Applicant can activate the paddy in the gut of shrimp that do not contaminate by research discovery Quercetin, rutin or tea polyphenols
Sweet peptide-S- the transferase (GST) of Guang, glucuronyl transferase (UGT), sulfotransferase (SULT) or aminopherase
(AANAT), therefore, the present invention protect first these antioxidants of Quercetin, rutin or tea polyphenols improve GST, UGT,
Application in the detoxicating activity of these detoxication enzymes of SULT or AANAT.
In addition, research is it has also been found that have Quercetin, rutin or tea polyphenols to the animal for having infected mycotoxin
Cut down the effect of its internal mycotoxin, therefore, the present invention also protects antioxidant mycotoxin residual in abatement animal body
In application, the antioxidant be selected from Quercetin, rutin or tea polyphenols；The mycotoxin is selected from T-2 toxin, DON poison
It is element, one of OTA toxin, two or more.
Meanwhile research is it has also been found that Quercetin, rutin or tea polyphenols can be improved the animal for having infected mycotoxin
Growth characteristics, therefore the present invention also protects application of the antioxidant in raising animal growth performance, the antioxidant is selected from
Quercetin, rutin or tea polyphenols.
Preferably, the animal is aquatic livestock；Specifically, the aquatic livestock is prawn.
Experimental data shows: T-2 toxin is 18.02 ± 2.41ng/g at prawn muscle residual quantity top；Using feed
Middle additive amount is the Quercetin of 1.6% (mass ratio), can make T-2 toxin residue in prawn muscle cut down 60.56 ±
5.77%；Preferably, when the amount ratio of T-2 toxin and Quercetin is 10.8~16.2mg:8.0 of per kilogram feed~16.0g, Mongolian oak
Pi Su has preferable consumption to T-2 toxin.
Use additive amount in feed the T-2 toxin residue in prawn muscle can be made to disappear for the rutin of 2.4% (mass ratio)
Subtract 84.26 ± 6.34%；Preferably, T-2 toxin and the amount ratio of rutin be 10.8~16.2mg:12.0 of per kilogram feed~
When 24.0g, rutin has preferable consumption to T-2 toxin.
Use additive amount in feed the T-2 toxin in prawn muscle can be made to remain for the tea polyphenols of 0.64% (mass ratio)
Amount abatement 77.63 ± 5.32%；The amount ratio of T-2 toxin and tea polyphenols is 16.2~24.3mg:3.2 of per kilogram feed~6.4g
When, tea polyphenols have preferable consumption to T-2 toxin.
DON toxin is 11.55 ± 1.05ng/g at prawn muscle residual quantity top；Use in feed additive amount for
The Quercetin of 3.2% (mass ratio) can make the DON toxin residue in prawn muscle cut down 48.90 ± 3.47%；Preferably,
When the amount ratio of DON toxin and Quercetin is 20.3~30.4mg:16.0 of per kilogram feed~32.0g, Quercetin is to DON toxin
There is preferable consumption.
Use additive amount in feed the DON toxin residue in prawn muscle can be made to disappear for the rutin of 4.8% (mass ratio)
Subtract 44.76 ± 4.08%；Preferably, DON toxin and the amount ratio of rutin be 20.3~30.4mg:24.0 of per kilogram feed~
When 48.0g, rutin has preferable consumption to DON toxin.
Use additive amount in feed the DON toxin in prawn muscle can be made to remain for the tea polyphenols of 0.64% (mass ratio)
Amount abatement 40.91 ± 3.86%；Preferably, the amount ratio of DON toxin and tea polyphenols is 20.3~30.4mg of per kilogram feed:
When 3.2~6.4g, tea polyphenols have preferable consumption to DON toxin.
OTA toxin is 11.19 ± 0.96ng/g at prawn muscle residual quantity top；Use in feed additive amount for
The Quercetin of 0.4% (mass ratio) can make the OTA toxin residue in prawn muscle cut down 37.39 ± 2.58%；Preferably,
When the amount ratio of OTA toxin and Quercetin is 2.0~3.0mg:2.0 of per kilogram feed~4.0g, Quercetin to OTA toxin have compared with
Good consumption.
Use additive amount in feed the OTA toxin residue in prawn muscle can be made to disappear for the rutin of 4.8% (mass ratio)
Subtract 39.47 ± 2.54%；Preferably, OTA toxin and the amount ratio of rutin be 4.5~6.8mg:24.0 of per kilogram feed~
When 48.0g, rutin has preferable consumption to OTA toxin.
Use additive amount in feed the OTA toxin in prawn muscle can be made to remain for the tea polyphenols of 0.16% (mass ratio)
Amount abatement 38.30 ± 3.15%；Preferably, the amount ratio of OTA toxin and tea polyphenols is 3.0~4.5mg:1.6 of per kilogram feed
When~3.2g, tea polyphenols have preferable consumption to OTA toxin.
Compared with prior art, the invention has the following advantages:
The present invention can activate the paddy in the gut of shrimp that do not contaminate by research discovery Quercetin, rutin or tea polyphenols
Sweet peptide-S- the transferase of Guang, glucuronyl transferase, aminopherase or sulfotransferase, to promote the life of II phase enzyme
Object conversion induces the activity of the contamination endogenous detoxication enzyme of gut of shrimp to accelerate prawn by adding inducer in prawn feed
The metabolism of vivotoxin reduces or removes the residual of toxin, it is ensured that prawn food safety.
Specific embodiment
The contents of the present invention are further illustrated below in conjunction with specific embodiment, but should not be construed as to limit of the invention
System.In the case where without departing substantially from spirit of that invention and essence, to modifications or substitutions made by the method for the present invention, step, condition, belong to
In the scope of the present invention.Unless otherwise noted, experimental method used in embodiment is well known to the skilled person
Conventional method and technology, reagent or material are to be obtained by commercial sources.
The detection method of tri- kinds of mycotoxins of T-2, OTA and DON of the present invention can refer to document Wenshuo Sun,
Zheng Hana,,Johan Aerts,et al.A reliable liquid chromatography tandem mass
spectrometry methodfor simultaneous determination of multiple mycotoxins in
fresh fi shand dried seafoods[J].Journal of Chromatography,2015,42-48。
Abatement of 1 inducer of embodiment to mycotoxin
One, mycotoxin contamination prawn to be measured
Experiment is with litopenaeus vannamei (Litopenaeus vannamei, L.vannamei, 6.5 ± 0.5g/ tail) from Zhan
East of a river island cultivation base.It transports laboratory back and is placed in the 150L plastic box equipped with about 2/3 volume seawater the oxygen of whole day exposure for 24 hours,
Every several 35 tails of case shrimp or so, temperature control is at 25 DEG C or so, and pH7.47~7.64, every morning, prawn culturing bottom was removed in timing
Excreta, replace 1/3 seawater.It is daily to raise with feeding prawn when 21:30 when 15:30 later when Beijing time 9:30
The amount of feeding is the 5% of prawn weight, and feeding volume three times per day is respectively 20%, 30% and the 50% of a daily amount, and feeding is temporarily supported
One week.
Prepare three kinds of mycotoxin microcapsules poison bait material respectively, test is contaminated using incremental dose method, with T-2 in feed,
OTA and DON content of toxins is respectively 4.8,6.0 and 1.78mg/kg as starting poisoning dosage, is contaminated daily, and 4 days are one week
Phase, each issue 1.5 times incremental, continuous contamination 20 days, and blank control is arranged.And prawn culturing case is collected when blowdown every morning
Middle residual feed is dried and is weighed.Period records the death condition and changes of weight of prawn, and calculates cumulative coefficient K value, simultaneously
It collects each phase contamination prawn different tissues (muscle, hepatopancrease, enteron aisle, shrimp head and blood) and is placed in -80 DEG C and save backup, be used for
Subsequent II relative keys enzyme activity (UGT, SULT, AANAT) and the remaining measurement of toxin, and to each phase prawn muscle, hepatopancrease and
Gut tissue sections carry out displaing microstructure observing.
Appropriate prawn tissue block (muscle, shrimp head, hepatopancrease and enteron aisle) is taken, weighing measures the sterile saline of pre-cooling,
The volume total amount of physiological saline is 9 times of tissue block weight, and ice bath is homogenized, and 10% homogenate is made in revolving speed 10,000r/min
Liquid is centrifuged 15min for 4 DEG C, 2500r/min of this homogenate, takes measurement of the centrifuged supernatant for enzyme activity.Supernatant is measured simultaneously
Protein content in liquid, using Coomassie brilliant G-250 method, using bovine serum albumin(BSA) as standard protein, measuring method reference
Bradford method.
The GSTs enzyme activity determination of prawn blood and tissue (muscle, shrimp head, hepatopancrease and enteron aisle) builds up reagent according to Nanjing
Box illustrates to carry out；The measurement of UGT, SULT and AANAT enzyme activity illustrates to carry out according to Shanghai grain husk heart experimental facilities kit.
Two, the feed containing inducer is prepared
The feed containing inducer is prepared, adds inducer (Quercetin, rutin and tea polyphenols) into prawn feed.Inducer
Additive amount also according to incremental principle, wherein the initial additive amount of Quercetin is 0.2% (mass fraction) of forage volume, and rutin is
0.3% (mass fraction), tea polyphenols are 0.04% (mass fraction), and the amount being added in feed is incremented by according to 2 times of amounts, and 4 days are 1
A period, continuous feeding 20 days, and blank control group prawn is set, induction experiment is carried out, collects each phase test prawn respectively
Different tissues (muscle, enteron aisle) are placed in -80 DEG C and save backup, and are used for the measurement II of II relative keys enzyme activity (UGT, SULT, AANAT)
Relative keys removing toxic substances enzyme activity.
(1) using LC-MS/MS detection ascending-dose contamination prawn different tissues organ toxicity's element residual, as a result are as follows: muscle
Middle mycotoxin residual highest numerical value difference T-2 is 51.89ng, and OTA 32.21ng, DON are 11.55 ± 1.03ng.
Depression effect of (2) three kinds of toxin to three kinds of detoxication enzymes of gut of shrimp
It is compared with prawn of not contaminating, T-2 toxin, OTA, DON mainly inhibit UGT/SULT/AANAT in gut of shrimp, maximum
Inhibition amplitude is respectively 51%/81%/87%；58%/68%/76%；71%/27%/42%.
(3) activation effect of three kinds of detoxication enzymes of inducer gut of shrimp
Inducer Quercetin, rutin, tea polyphenols to the activation of UGT/SULT/AANAT in gut of shrimp of not contaminating most substantially
Spending (relative to control group) is respectively 2.5 times/3.1 times/3.7 times；2.6 times/4.3 times/3.8 times；2.7 times/4.6 times/3.7 times.
(4) antagonistic effect of inducer activation and four kinds of toxin inhibiting effect based on three kinds of detoxication enzymes of gut of shrimp
On this basis, inducer (feed of the feeding containing inducer) is to the detoxication enzyme in different toxin contamination gut of shrimp
Induction amplitude is promoted, if the contamination group of inducer is not added to be control group.Concrete outcome is as follows:
A. in the gut of shrimp of inducer Quercetin (feeding 16d) activation contamination T-2 toxin, OTA, DON UGT SULT
The amplitude peak of AANAT detoxication enzyme is respectively up to 4.6 times/7.6 times/7.7 times；3.7 times/5.2 times/9.0 times (reach for short-term 4 days
It is maximum)；2.9 times/4.9 times/4.1 times.
B. in the gut of shrimp of inducer rutin (feeding 16d) activation contamination T-2 toxin, OTA, DON UGT SULT
The amplitude peak of AANAT detoxication enzyme is respectively up to 3.4 times/5.1 times/4.9 times；2.4 times/4.2 times/3.2 times；4.6 times/6.9 times/
6.7 again.
C. in the gut of shrimp of inducer tea polyphenols (feeding 20d) activation contamination T-2 toxin, OTA, DON UGT SULT
The amplitude peak of AANAT detoxication enzyme is respectively up to 3.0 times/4.8 times/4.6 times；3.3 times/4.9 times/4.8 times；4.6 times/6.9 times/
6.7 again.
(5) three kinds of toxin remain fall in prawn muscle under the antagonistic effect that inducer activation inhibits with three kinds of toxin
For the prawn of contamination, it is the poison bait material containing inducer and the poison without inducer respectively that the later period, which feeds different groups of other feeds,
Bait (setting control group), final result show that toxin residual fall is respectively as follows: in the muscle of contamination prawn
A. inducer Quercetin and three kinds of toxin antagonisms and after activating three kinds of detoxication enzymes of enteron aisle, T-2 toxin in muscle, OTA,
DON residual quantity resolution rate is up to 60%, 66% (short-term effective), 49%.
B. inducer rutin and three kinds of toxin antagonisms and after activating three kinds of detoxication enzymes of enteron aisle, T-2 toxin in muscle, OTA,
DON residual quantity resolution rate is up to 84%, 87%, 45% (only permanently effective).
C. inducer tea polyphenols and three kinds of toxin antagonisms and after activating three kinds of detoxication enzymes of enteron aisle, T-2 toxin in muscle, OTA,
DON residual quantity resolution rate is up to 78%, 40% (only permanently effective), 49%.
Influence of 2 inducer of embodiment to prawn growth characteristics
During three kinds of mycotoxin ascending-dose viral infection tests, prawn residual feed, drying are collected every morning.And in
Prawn weight is measured after exposed each phase and calculates grazing rate (FR), rate of body weight gain (WG), specific growth rate (SGR), is counted
It is as follows to calculate formula:
Grazing rate (FR, %Wd-1)=100 × F/d/ [((W0+Wi)/2]
Rate of body weight gain (WG, %)=[Wi-W0]/W0
Specific growth rate (SGR, %)=100 × [lnWi-lnW0]/d
F- total food intake, dry weight, g in formula；D- number of days, day；W- tests prawn weight, weight in wet base, g；W0Test prawn
Original body mass, weight in wet base, g；WiThe weight of i-th day prawn of on-test, weight in wet base, g.
Survival rate is investigated: in 20 days incremental viral infection tests, the survival rate of control group prawn is always 90% or more, and T-
2 toxin contamination group prawns survival rate at the 5th~8 day is 84.76%, is lower than 90%, downward trend is presented always later, test
At the end of survival rate be only 49.52%, The dead quantity is more than half；Survival rate is 47.62% when DON contamination group prawn off-test,
Lower than the half of initial quantity.OTA contamination group is 58.10% in the survival rate of contamination final stage, i.e. the death rate is less than just
The half of beginning quantity.
Rate of body weight gain and specific growth rate are investigated: the rate of body weight gain and specific growth rate of T-2 toxin contamination group prawn are with test
Carry out significantly lower than control group.It is 0.64% that T-2 toxin contamination group prawn, which reaches minimum specific growth rate, during test.
The rate of body weight gain rate of change of DON contamination group prawn is slower and slower, differs increasing with control group.The spy of DON contamination group prawn
Determine that the growth rate later period is almost unchanged, the entire specific growth rate for testing process is always below control group.OTA contamination group prawn
Rate of body weight gain is increasing always, but rate of gain does not increase persistently, and prawn growth is slower.The specific growth rate of prawn is 0.50
Change between~0.90%, lower than the prawn of control group.
Grazing rate is investigated: the grazing rate of control group prawn maintains always 4% or more, and T-2 toxin contamination group prawn
Grazing rate is declining always.The grazing rate of DON contamination group prawn drops to always 2.75% or so, the rate of body weight gain change of this and prawn
Slow trend is consistent, and with the accumulation of contamination amount, the i.e. increase of contamination time, test group is differed with the grazing rate of control group and got over
Come bigger.The daily scale of feeding of prawn is the 5% of its weight, and the grazing rate of OTA contamination prawn group wave in 3.50% range
Dynamic, grazing rate reduces.
Inducer the results are shown in Table 1 to prawn growth characteristics index, compared to control group, Quercetin, rutin and tea polyphenols group
Prawn survival rate, rate of body weight gain, specific growth rate and grazing rate have a degree of decline, and Quercetin group fall is minimum,
Close to Normal group.
Inducer and toxin collective effect group can be improved the survival rate of cultured prawn, test initial stage, inducer and toxin
The variation of collective effect group prawn relative survival rate less, 1.00 or so, with the progress of test, survive by the two collective effect group
Rate is gradually higher than control, at the end of contamination, Quercetin-AFB1With tea polyphenols-AFB1Group prawn is compared to AFB1The survival of contamination group
Rate is 1.77.
Above result indicate that inducer and toxin collective effect influence less, in addition to tea polyphenols-T-2 the rate of body weight gain of prawn
Group prawn body weight increase rate when testing the period 5 reduces, and is 0.95, and the weight gain of other collective effect group prawns, which is in, stablizes
State.
At the initial stage of contamination, inducer acts on contamination prawn, and the relatively specific growth rate of T-2 toxin contamination group prawn is most
Low, in 0.58~0.66 range, the opposite specific growth rate of inducer and DON and OTA toxin collective effect group prawn exists
It is changed in 0.90~1.14 range, with the progress of test, the spy relatively of three kinds of inducers and T-2 toxin collective effect group prawn
Determine growth rate to gradually rise, and the relatively specific growth rate of other toxin and inducer joint group prawn keeps stablizing.
For grazing rate, inducer and toxin collective effect group, grazing rate of three kinds of inducers to OTA toxin contamination prawn
Maximum is influenced, the grazing rate of detoxifying function group prawn is improved, other group variations are little.
Influence of 1 ascending-dose method of the table contamination mycotoxin to prawn growth indexes
Claims (1)
1. application of the antioxidant in abatement animal body in mycotoxin residual, which is characterized in that the antioxidant is selected from
Quercetin, rutin or tea polyphenols；The mycotoxin be selected from one of T-2 toxin, DON toxin, OTA toxin, two kinds or
Person is two or more；
The Quercetin, rutin, tea polyphenols and T-2 toxin mass ratio be respectively (8.0~16.0g:10.8~16.2mg),
(12.0~24.0g:10.8~16.2mg), (3.2~6.4g:16.2~24.3mg)；
Quercetin, rutin, tea polyphenols and DON toxin mass ratio be respectively (16.0~32.0g:20.3~30.4mg), (24.0
~48.0g:20.3~30.4mg), (3.2~6.4g:20.3~30.4mg)；
Quercetin, rutin, tea polyphenols and OTA toxin mass ratio be respectively (2.0~4.0g:2.0~3.0mg), (24.0~
48.0g:4.5~6.8mg), (1.6~3.2g:3.0~4.5mg),
The animal is prawn.
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| CN111467476B (en) * | 2020-03-05 | 2021-07-27 | 山东农之安生物科技有限公司 | Antidote pharmaceutical composition and use thereof |
| CN113966788A (en) * | 2021-11-03 | 2022-01-25 | 武汉轻工大学 | Composition for relieving pig vomitoxin poisoning, feed additive, feed and preparation method |
| CN115671094A (en) * | 2022-11-01 | 2023-02-03 | 四川农业大学 | Application of quercetin in preparation of anti-mycotoxin medicines |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101854953A (en) * | 2007-09-11 | 2010-10-06 | 乔斯林糖尿病中心股份有限公司 | Phase II detoxification and antioxidant activity |
| CN101999545A (en) * | 2010-12-01 | 2011-04-06 | 北京资源亚太饲料科技有限公司 | Compound feed for lactating sows and preparation method thereof |
| CN102845606A (en) * | 2012-08-06 | 2013-01-02 | 王茜 | Novel ammonia-reduction mould-removal feed additive |
| CN104522390A (en) * | 2014-12-22 | 2015-04-22 | 天津市茂林水产养殖有限公司 | Feed additive for industrial prawn aquaculture |
| CN105713865A (en) * | 2014-12-05 | 2016-06-29 | 上海工业生物技术研发中心 | Microorganism having detoxifizyme activity and with fermentation residues applicable to feed |
-
2016
- 2016-08-08 CN CN201610641667.9A patent/CN106260742B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101854953A (en) * | 2007-09-11 | 2010-10-06 | 乔斯林糖尿病中心股份有限公司 | Phase II detoxification and antioxidant activity |
| CN101999545A (en) * | 2010-12-01 | 2011-04-06 | 北京资源亚太饲料科技有限公司 | Compound feed for lactating sows and preparation method thereof |
| CN102845606A (en) * | 2012-08-06 | 2013-01-02 | 王茜 | Novel ammonia-reduction mould-removal feed additive |
| CN105713865A (en) * | 2014-12-05 | 2016-06-29 | 上海工业生物技术研发中心 | Microorganism having detoxifizyme activity and with fermentation residues applicable to feed |
| CN104522390A (en) * | 2014-12-22 | 2015-04-22 | 天津市茂林水产养殖有限公司 | Feed additive for industrial prawn aquaculture |
Non-Patent Citations (4)
| Title |
|---|
| Effects of Rutin from Toona sinensis on the immune and physiological responses of white shrimp (Litopenaeus vannamei) under Vibrio alginolyticus challenge;Hsieh 等;《Fish & Shellfish Immunology》;20081231(第25期);第581-588页 摘要 * |
| Flavonoids Protect Against T-2 Mycotoxins both in vitro and in vivo;R.J.F. Markham 等;《Journal of General Microbiology》;19871231;1589-1592页 摘要 * |
| 槲皮素和芦丁在家蚕幼虫中对解毒酶的诱导模式;张月娥;《重庆大学硕士学位论文》;20121130;摘要、第25-26页 第3.2.2节 * |
| 茶多酚、茶色素对肝癌癌前病变大鼠肝脏Ⅱ相解毒酶的诱导及其在化学预防中的作用;宫芸芸 等;《卫生研究》;20000520;第29卷(第3期);第159-161页 摘要、第2.2节 * |
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