CN106259850A - Can inhibition of potato reducing sugar accumulation storage practice - Google Patents

Can inhibition of potato reducing sugar accumulation storage practice Download PDF

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Publication number
CN106259850A
CN106259850A CN201610670307.1A CN201610670307A CN106259850A CN 106259850 A CN106259850 A CN 106259850A CN 201610670307 A CN201610670307 A CN 201610670307A CN 106259850 A CN106259850 A CN 106259850A
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potato
reducing sugar
inhibition
rhizoma solani
solani tuber
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CN106259850B (en
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林琼
王志东
解雅晶
张洁
刘伟
解新方
关文强
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Institute of Food Science and Technology of CAAS
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00Preservation or chemical ripening of fruit or vegetables
    • A23B7/015Preserving by irradiation or electric treatment without heating effect
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00Preservation or chemical ripening of fruit or vegetables
    • A23B7/14Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
    • A23B7/153Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
    • A23B7/154Organic compounds; Microorganisms; Enzymes

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
  • Storage Of Harvested Produce (AREA)
  • Storage Of Fruits Or Vegetables (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of can the storage practice of inhibition of potato reducing sugar accumulation, wherein, Rhizoma Solani tuber osi is carried out for the first time ultra-vioket radiation 13 hours with 250~258nm ultraviolet light, then in relative humidity be 90 95%, temperature be 26 DEG C under the conditions of storage.The present invention can the effectively accumulation of reducing sugar during inhibition of potato storage, and other indexs such as the outward appearance of Rhizoma Solani tuber osi, weight-loss ratio can be made to keep good, and there is simple to operate, easy to use, the advantage of pretreatment low cost, being that a whole set of of reducing sugar accumulation improves system during inhibition of potato cryopreservation, the storage to Rhizoma Solani tuber osi has important using value.

Description

Can inhibition of potato reducing sugar accumulation storage practice
Technical field
The present invention relates to Rhizoma Solani tuber osi postharvest storage and quality control technique field, particularly to one ultra-vioket radiation Ma Ling Potato suppresses the preprocess method of reducing sugar accumulation during its cryopreservation.
Background technology
Rhizoma Solani tuber osi is the fourth-largest cereal crops of China, is also important vegetable, feedstuff and insutrial crop simultaneously.? High temperature, high humidity holding conditions under, potato tubers easy susceptible rot, less than 0 DEG C again can cold injury, environmental drying is easily dehydrated and withers Here, therefore, Storing Condition of Seed-potatoes requires the strictest.
Cryopreservation is the major measure extending the Potato ring rot bacteria cycle, but under the conditions of cryopreservation, the intracellular shallow lake of tuber Balance between powder metabolism, carbohydrate metabolism, glycolysis and Repiration is broken, and reducing sugar accumulates in a large number, has had a strong impact on Ma Ling The processing quality of potato.
It is mainly distributed on around eye different from the accumulation of reducing sugar in tuber germination process, during Rhizoma Solani tuber osi cryopreservation The reducing sugar produced is distributed in whole tuber, when the fried processing of potato chips, French fries high temperature, reducing sugar free aldehyde with There is the Maillard reaction (Shallenberger etc., 1959) of non-enzymatic in the free a-amino acid of aminoacid or protein, leads Product generation brown stain, mouthfeel that cause is produced are bitter and astringent.Research is had to confirm, along with a large amount of Maillard reaction end-products Appearance, also create a kind of potential neurotoxin and carcinogen acrylamide, its content and the middle reducing sugar that processes raw material Content become positive correlation (Mottram etc., 2002;Sanny etc., 2012).It would therefore be highly desirable to find new storage of potato side Method, reducing sugar accumulation during preserving with inhibition of potato, keep the original quality of Rhizoma Solani tuber osi.
Summary of the invention
It is an object of the invention to solve at least the above and/or defect, and provide the most following excellent by illustrate Point.
Another object of the present invention provide a kind of can the storage practice of inhibition of potato reducing sugar accumulation, it is possible to effectively The accumulation of reducing sugar in inhibition of potato storage.
Other purposes with some for achieving the above object, the present invention adopts the following technical scheme that
A kind of can the storage practice of inhibition of potato reducing sugar accumulation, wherein, by Rhizoma Solani tuber osi by 250~258nm ultraviolets Light carries out for the first time ultra-vioket radiation 1-3 hour, is then 90-95% in relative humidity, and temperature is preserved under the conditions of being 2-6 DEG C.
Preferably, described can be in the storage practice of inhibition of potato reducing sugar accumulation, at storage of potato 26-30 Carrying out second time ultra-vioket radiation after it, then proceeding in relative humidity is 90-95%, and temperature is preserved under the conditions of being 2-6 DEG C;Its In, described second time ultra-vioket radiation method is that 250~258nm UV illumination penetrate 1-3 hour.
Preferably, described can be in the storage practice of inhibition of potato reducing sugar accumulation, Rhizoma Solani tuber osi is used by pre-irradiation Suckering agents is smeared;Containing 0.03-0.08% maleic hydrazide, 0.005-0.02% naphthalene methyl acetate, 0.005-in described Suckering agents 0.02% chlorpropham and 10-60% plant ash.
Preferably, described can the storage practice of inhibition of potato reducing sugar accumulation contain in described Suckering agents 0.05% maleic hydrazide, 0.01% naphthalene methyl acetate, 0.01% chlorpropham and 30% plant ash.
Preferably, described can be in the storage practice of inhibition of potato reducing sugar accumulation, described first time ultraviolet is shone Rhizoma Solani tuber osi after irradiating after penetrating at room temperature is placed 20-30 hour, then preserves.
Preferably, described can be in the storage practice of inhibition of potato reducing sugar accumulation, described first time ultraviolet is shone Penetrating and penetrate 2 hours by 254nm UV illumination, reserve temperature is 4 DEG C.
The present invention at least includes following beneficial effect: the present invention is by carrying out certain condition before Rhizoma Solani tuber osi preserves Ultra-vioket radiation pretreatment, can effectively reducing sugar accumulation in inhibition of potato storage, through verification experimental verification, after preserving 3 days, It is respectively 0.48mg/g and 0.33mg/g dry weight according to glucose and fructose content in the Rhizoma Solani tuber osi of the method for the invention storage, 39% and 37% is reduced respectively relative to matched group Rhizoma Solani tuber osi;After preserving 28 days, according to the horse of the method for the invention storage In bell potato, glucose and fructose content are respectively 2.12mg/g and 1.68mg/g dry weight, reduce respectively relative to matched group Rhizoma Solani tuber osi 43% and 35%;After preserving 28 days, total reducing sugars content is 3.92mg/g dry weight, relative to total reduction of matched group Rhizoma Solani tuber osi Sugar content reduces 39%.
The present invention can make other indexs such as the outward appearance of Rhizoma Solani tuber osi, weight-loss ratio keep good simultaneously, and have simple to operate, Easy to use, the advantage of pretreatment low cost, is that a whole set of of reducing sugar accumulation improves body during inhibition of potato cryopreservation System, the storage to Rhizoma Solani tuber osi has important using value.
Part is embodied by the further advantage of the present invention, target and feature by description below, and part also will be by this Invention research and practice and be understood by the person skilled in the art.
Accompanying drawing explanation
Fig. 1 is Rhizoma Solani tuber osi weight-loss ratio and yield of carbon dioxide rate of change cartogram;
Fig. 2 is Rhizoma Solani tuber osi total reducing sugars changes of contents curve chart;
Fig. 3 is fructose, glucose, sucrose, the galactose changes of contents curve chart during preserving in Rhizoma Solani tuber osi.
Detailed description of the invention
Below in conjunction with the accompanying drawings and the present invention is elaborated by embodiment, to make those of ordinary skill in the art refer to this theory Can implement according to this after bright book.
A kind of can the storage practice of inhibition of potato reducing sugar accumulation, wherein, by Rhizoma Solani tuber osi by 250~258nm ultraviolets Light carries out for the first time ultra-vioket radiation 1-3 hour, is then 90-95% in relative humidity, and temperature is preserved under the conditions of being 2-6 DEG C. The present invention, can effectively inhibition of potato storage by carrying out the ultra-vioket radiation pretreatment of certain condition before Rhizoma Solani tuber osi preserves Reducing sugar accumulation during Tibetan, can make other indexs such as the outward appearance of Rhizoma Solani tuber osi, weight-loss ratio keep good simultaneously, and have operation Simply, easy to use, the advantage of pretreatment low cost, be that a whole set of of reducing sugar accumulation is complete during inhibition of potato cryopreservation Kind system, the storage to Rhizoma Solani tuber osi has important using value.
Described can the storage practice of inhibition of potato reducing sugar accumulation be carried out after 26-30 days at storage of potato Ultra-vioket radiation for the second time, then proceeding in relative humidity is 90-95%, and temperature is preserved under the conditions of being 2-6 DEG C;Wherein, described Secondary ultra-vioket radiation method is that 250~258nm UV illumination penetrate 1-3 hour.Through secondary ultra-vioket radiation, it is possible to more effectively Improve the inhibition of reducing sugar accumulation in Rhizoma Solani tuber osi.
Described can be in the storage practice of inhibition of potato reducing sugar accumulation, Rhizoma Solani tuber osi Suckering agents is coated with by pre-irradiation Smear;Containing 0.03-0.08% maleic hydrazide, 0.005-0.02% naphthalene methyl acetate, 0.005-0.02% chlorobenzene in described Suckering agents Amine spirit and 10-60% plant ash.Described Suckering agents can sprout by inhibition of potato, and the storage of potato phase is extended to 6 months Above.Described can be in the storage practice of inhibition of potato reducing sugar accumulation, after irradiating after described first time ultra-vioket radiation Rhizoma Solani tuber osi at room temperature place 20-30 hour, wait Rhizoma Solani tuber osi recover normal physiological metabolism, then preserve.
Embodiment 1
1, UV-C test group: by the Atlantic potato gathered through ultraviolet treatment with irradiation 2 hours, ultraviolet wavelength 254nm, Irradiate in transferring to the storeroom that holding conditions is temperature 4 degrees Celsius, relative air humidity 92% after terminating and continue storage.
CK matched group: by the Atlantic potato gathered directly in temperature 4 degrees Celsius, the storage of relative air humidity 92% The Rhizoma Solani tuber osi of indoor storage is comparison.
2, during this period, to the storage outward appearance of Rhizoma Solani tuber osi, weight-loss ratio, breathing rate, total reducing sugars, glucose, fructose, sugarcane The indexs such as sugar and galactose are tracked detection.
Wherein, Rhizoma Solani tuber osi weight-loss ratio assay method is: randomly select 15 Rhizoma Solani tuber osis, carries out labelling, measures single fruit weight (g), fruit weight-loss ratio=(initial weight-gravimetry)/initial weight × 100%.
Rhizoma Solani tuber osi respiratory intensity measures: often group test group and matched group are chosen 3 Rhizoma Solani tuber osis and be placed in same container airtight After 2h, measuring CO2 concentration with CO2 analyser (DanSenser), respiratory intensity changes scale with CO2 percent by volume per hour Show.
Rhizoma Solani tuber osi soluble sugar extracts and measures: often group test group and matched group weigh 200mg lyophilization powder, add 4ml 80% ethanol, mixing.Supersound extraction 30min, under room temperature 10,000rpm is centrifuged 15min, takes supernatant.Repeat to extract 2 Secondary, merge supernatant, be settled to 10ml.Taking 1ml extracting solution, add 1ml distilled water and dissolve, cross film after being evaporated, filtrate is used for ion Chromatograph measures soluble sugar content.The chromatographic condition that soluble sugar content measures is: use Carbo PacTMPA20 analytical column (3mm × 150mm), column temperature 35 DEG C, flow velocity is 0.5mL/min, and sampling volume is 10 μ L;Condition of gradient elution: A is pure water, B For the NaOH of 250mM, 0-20min, 92.5%A, 7.5%B isocratic elution;Detector is pulsed amperometry, gold electrode.
3, experimental result
Fig. 1 is Rhizoma Solani tuber osi weight-loss ratio and yield of carbon dioxide rate of change cartogram.From fig. 1, it can be seen that Atlantic potato exists Under the conditions of common storage, weight-loss ratio is slow ascendant trend, and (as shown in Figure 1A, wherein CK is to reach 0.67% after preserving 28 days Comparison, UV-C is test group);Rhizoma Solani tuber osi CO before treatment2Generating rate is higher, declines rapidly, storage after proceeding to cold preservation Middle change relatively steadily (as shown in Figure 1B, wherein CK is comparison, and UV-C is test group).Atlantic potato is through UV-C process, storage After hiding 28 days, exterior quality and matched group are without significant difference.UV-C process group weight-loss ratio in storage is in rising trend, with Contrast ratio is without significant difference (as described in Figure 1A);Rhizoma Solani tuber osi breathing rate after warehouse-in that UV-C processes reduces rapidly, but UV-C Processing the breathing rate exacerbating fruit, during storage of potato, its breathing rate is significantly higher than matched group (such as Figure 1B institute Show).
Fig. 2 is Rhizoma Solani tuber osi total reducing sugars changes of contents curve chart.As shown in Figure 2 (CK is comparison, and UV-C is test group): big The West Rhizoma Solani tuber osi is under the conditions of common storage, and in Rhizoma Solani tuber osi, total reducing sugars content is relatively steady in preserving 7 days, notable by the 28th day Raise, initial value 1.32mg/g dry weight be increased to 6.48mg/g dry weight;During UV-C processes suppression Atlantic potato storage The accumulation of total reducing sugars content, after preserving 28 days, total reducing sugars content is 3.92mg/g dry weight, drops relative to matched group Rhizoma Solani tuber osi Low by 39%.
Fig. 3 be fructose (as shown in Figure 3A) in Rhizoma Solani tuber osi, glucose (as shown in Figure 3 B), sucrose (as shown in Figure 3 C), half The lactose (as shown in Figure 3 D) changes of contents curve chart during preserving, wherein CK is comparison, and UV-C is test group.Can by Fig. 3 Know: storage of potato is after 3 days, and glucose and fructose content are respectively 0.48mg/g and 0.33mg/g dry weight, relative to matched group Rhizoma Solani tuber osi reduces 39% and 37% respectively;After preserving 28 days, UV-C processes potato glucose and fructose content is respectively 2.12mg/g and 1.68mg/g dry weight, reduces 43% and 35% respectively relative to matched group Rhizoma Solani tuber osi;And big west in matched group During the storage of potato of ocean, fructose content is rapid increase trend, reaches 2.60mg/g dry weight after preserving 28 days;Glucose content The most in rising trend, reach 3.75mg/g dry weight after preserving 28 days;Cane sugar content is the highest in the whole storage stage of Rhizoma Solani tuber osi, It is the main soluble sugar component in potato tubers, in rising trend in storage, reach after preserving 28 days 59.11mg/g dry weight, UV-C processes and has no significant effect sucrose in Rhizoma Solani tuber osi (as shown in Figure 3 C) content;Galactose content exists Storage of potato process is faint downward trend, and the later stage rises again, UV-C process after in Rhizoma Solani tuber osi galactose content under slowly Fall trend, with contrast ratio without significant difference (as shown in Figure 3 D).
Owing to fructose and glucose are main reducing sugar kind in Atlantic potato, the rising of its content directly results in always The rising of content of reducing sugar, has a strong impact on Potato ring rot bacteria quality.
Embodiment 2
By the Atlantic potato gathered through ultraviolet treatment with irradiation 1 hour, ultraviolet wavelength 254nm, irradiate and turn after terminating Storage is continued in moving on to the storeroom that holding conditions is temperature 4 degrees Celsius, relative air humidity 90-95%.
Embodiment 3
By the Atlantic potato gathered through ultraviolet treatment with irradiation 3 hours, ultraviolet wavelength 254nm, irradiate and turn after terminating Storage is continued in moving on to the storeroom that holding conditions is temperature 4 degrees Celsius, relative air humidity 90-95%.UV-C processes Ma Ling Potato preserve after 1,2,3 hours 28 days, 60 days and after 90 days statistical result as shown in table 1.
Table 1 UV-C processes statistical result after Rhizoma Solani tuber osi preserved 28,60 and 90 days after 1,2,3 hours
Embodiment 4
1, Rhizoma Solani tuber osi is carried out ultra-vioket radiation 2 hours for the first time with 250~258nm ultraviolet light, then in relative humidity For 90-95%, temperature is preserved under the conditions of being 2-6 DEG C.
2, carry out second time ultra-vioket radiation after 26-30 days at storage of potato, then proceed in relative humidity be 90- 95%, temperature is preserved under the conditions of being 2-6 DEG C;Wherein, described second time ultra-vioket radiation method is 250~258nm UV illumination Penetrate 2 hours.
After UV-C carries out 2 hours irradiating, storage of potato phase bud ratio, weight-loss ratio, total reducing sugars content data are shown in Table 2, its In within 0 day, carry out for the first time UV-C and irradiate, carry out second time UV-C after 28 days and irradiate.Experimental result is as shown in table 2.
Table 2
Although embodiment of the present invention are disclosed as above, but it is not restricted in description and embodiment listed Using, it can be applied to various applicable the field of the invention completely, for those skilled in the art, and can be easily Realizing other amendment, therefore under the general concept limited without departing substantially from claim and equivalency range, the present invention does not limit In specific details with shown here as the legend with description.

Claims (6)

1. can the storage practice of inhibition of potato reducing sugar accumulation, wherein, by Rhizoma Solani tuber osi with 250~258nm uviol lamps Light carries out for the first time ultra-vioket radiation 1-3 hour, is then 90-95% in relative humidity, and temperature is preserved under the conditions of being 2-6 DEG C.
The most as claimed in claim 1 can inhibition of potato reducing sugar accumulation storage practice, wherein, at storage of potato Carrying out second time ultra-vioket radiation after 26-30 days, then proceeding in relative humidity is 90-95%, and temperature is store under the conditions of being 2-6 DEG C Hide;Wherein, described second time ultra-vioket radiation method is that 250~258nm UV illumination penetrate 1-3 hour.
The most as claimed in claim 1 can inhibition of potato reducing sugar accumulation storage practice, wherein, pre-irradiation is by Rhizoma Solani tuber osi Smear with Suckering agents;Containing 0.03-0.08% maleic hydrazide, 0.005-0.02% naphthalene methyl acetate, 0.005-in described Suckering agents 0.02% chlorpropham and 10-60% plant ash.
The most as claimed in claim 3 can inhibition of potato reducing sugar accumulation storage practice, wherein, described Suckering agents contains There are 0.05% maleic hydrazide, 0.01% naphthalene methyl acetate, 0.01% chlorpropham and 30% plant ash.
The most as claimed in claim 1 can inhibition of potato reducing sugar accumulation storage practice, wherein, described first time ultraviolet Rhizoma Solani tuber osi after irradiating after irradiation is at room temperature placed 20-30 hour, then preserves.
The most as claimed in claim 1 can inhibition of potato reducing sugar accumulation storage practice, wherein, described first time ultraviolet Irradiation 254nm UV illumination penetrates 2 hours, and reserve temperature is 4 DEG C.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108522499A (en) * 2018-06-13 2018-09-14 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) A method of improving sweet potato potato seed storage quality
CN113575673A (en) * 2021-06-30 2021-11-02 中国农业科学院农产品加工研究所 Gradient hypoxia cooperative gasification fumigation low temperature resistant saccharification storage method for potatoes

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105638857A (en) * 2015-12-31 2016-06-08 中国农业科学院农产品加工研究所 Pretreatment method for inhibiting reducing sugar accumulation during potato low-temperature storage period
CN105746683A (en) * 2016-03-01 2016-07-13 中国农业科学院农产品加工研究所 Storage and refreshing method of fresh-cut potato

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105638857A (en) * 2015-12-31 2016-06-08 中国农业科学院农产品加工研究所 Pretreatment method for inhibiting reducing sugar accumulation during potato low-temperature storage period
CN105746683A (en) * 2016-03-01 2016-07-13 中国农业科学院农产品加工研究所 Storage and refreshing method of fresh-cut potato

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108522499A (en) * 2018-06-13 2018-09-14 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) A method of improving sweet potato potato seed storage quality
CN113575673A (en) * 2021-06-30 2021-11-02 中国农业科学院农产品加工研究所 Gradient hypoxia cooperative gasification fumigation low temperature resistant saccharification storage method for potatoes
CN113575673B (en) * 2021-06-30 2022-06-07 中国农业科学院农产品加工研究所 Gradient hypoxia cooperative gasification fumigation low temperature resistant saccharification storage method for potatoes

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