CN106244646A - The preparation method of Blood index Bacterial cellulose - Google Patents
The preparation method of Blood index Bacterial cellulose Download PDFInfo
- Publication number
- CN106244646A CN106244646A CN201610829248.8A CN201610829248A CN106244646A CN 106244646 A CN106244646 A CN 106244646A CN 201610829248 A CN201610829248 A CN 201610829248A CN 106244646 A CN106244646 A CN 106244646A
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- China
- Prior art keywords
- bacterial cellulose
- preparation
- distilled water
- blood
- blood index
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3679—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits by absorption
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/24—Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
Abstract
The preparation method of Blood index Bacterial cellulose, for uremic blood purification treatment, it is characterized in that: in the culture medium of the SH that the ratio that the acetobacter xylinum seed liquor of shaken cultivation 24h is 6% by mass percentage is inoculated in improvement, at 30 DEG C, quiescent culture 7 days, obtain bacterial cellulose wet-coating；Bacterial cellulose wet-coating uses after flowing water is flushed distilled water rinse, then uses 1% sodium hydroxide solution to boil 4 hours, then soak half an hour in 0.5% acetic acid at room temperature after flowing water flushing, clean with distilled water flushing, dry.Preparation method of the present invention is simple, with low cost；Blood index Bacterial cellulose blood compatibility prepared by the present invention is good.Blood index Bacterial cellulose prepared by the present invention has a extensive future, and can reach therapeutic purposes, can alleviate again the financial burden of patient.
Description
Technical field
The invention belongs to medical material, be mainly used in the Blood index material of uremia's blood purification, be specifically related to one
The preparation method of the Bacterial cellulose of Blood index material.
Background technology
The life and health of people in uremia's serious threat, and at present, treatment uremia in latter stage mainly takes renal transplantation and blood
Liquid purification method.Owing to kidney source, the whole world is not enough or patient's self reason, renal transplantation can only be applied under special circumstances, the whole world
The blood purifications such as dependence hemodialysis, Blood index are sustained life by annual more than 1,000,000 patients.Hemodialysis is uremia
The most frequently used replacement therapy method, to removing, micromolecular water dissolubility toxin is largely effective, and to middle molecule toxins elimination effect not
Good.Therefore develop efficient medium molecular substance adsorbent, will be divided in being detained in uremic patient body by hemoperfusion mode
Sub-toxic removal, can efficiently control and treat uremia.Improve life in patients, have great importance undoubtedly.
Polysaccharide adsorbing material shows growth momentum faster in recent years.The cellulose synthesized by acetobacter antibacterial
Can produce substantial amounts of film surface, the hydrogen bond between fiber element gives its biggest mechanical strength, and purity is high, Bacterial cellulose table
Mask porous.Culture medium uses different monosaccharide or polysaccharide as carbon source, by regulating the carbohydrate metabolism approach of acetobacter xylinum, reaches
Control to product aperture, structure synthesizes, and the aperture on the one hand meeting middle molecule toxins adsorbing material requires (4-10nm) and increases
Strong adsorption, improves again the blood compatibility of Bacterial cellulose simultaneously.
Summary of the invention
It is contemplated that seek the technical method of a kind of Blood index Bacterial cellulose controllable biological synthesis.For uremia
Blood purification provides novel adsorbing material, alleviates the misery of uremic patient, extending life, improves life quality.
The present invention provides the compatibility good, and processing technology is simple, a kind of Blood index Bacterial cellulose with low cost
Preparation method.
Technical scheme: the preparation method of Blood index Bacterial cellulose, it is characterised in that: by shaken cultivation
The acetobacter xylinum seed liquor of 24h is in the culture medium of the ratio SH that is inoculated in improvement of 6% by mass percentage, quiet at 30 DEG C
Put cultivation 7 days, obtain bacterial cellulose wet-coating；Bacterial cellulose wet-coating use after flowing water is flushed distilled water rinse, so
Rear employing 1% sodium hydroxide solution boils 4 hours, then soaks half an hour in 0.5% acetic acid at room temperature after flowing water flushing, uses
Distilled water flushing is clean, dries.
The preparation method of the SH culture medium of described improvement is: by distilled water 1L, sugar 20 ~ 30g, peptone 7.5 ~ 10 g, ferment
Female powder 7.5 ~ 10 g, disodium hydrogen phosphate 7.5 ~ 10 g, citric acid 0.5 ~ 1.5 g and pH5.0 ~ 6.0 mix homogeneously becomes improvement
SH culture medium.
The preparation method of described acetobacter xylinum seed liquor is: by acetobacter xylinum list colony inoculation in the SH culture medium of improvement
In, shaken cultivation 24h becomes acetobacter xylinum seed liquor.
Described Bacterial cellulose is to control antibacterial fibre by dosage and the ratio of sugar in regulation acetobacter xylinum cultivating system
Dimension element aperture and physicochemical property.
The material that described sugar is glucose or monosaccharide or polysaccharide or several different carbon source material synthesizes in proportion.
Described monosaccharide includes glucose.
Described polysaccharide includes glucosamine.
Above-mentioned bacteria cellulose film, it removes the toxin application in blood as medicinal blood scavenging material.
Preparation method of the present invention is simple, with low cost；Blood index Bacterial cellulose blood prepared by the present invention
The compatibility is good.Blood index Bacterial cellulose prepared by the present invention has a extensive future, and can reach therapeutic purposes, can subtract again
The financial burden of light patient.
Accompanying drawing explanation
Fig. 1 is with glucose for carbon source biosynthetic Bacterial cellulose infrared spectrogram；
Fig. 2 is with glucose and glucosamine for carbon source biosynthetic Bacterial cellulose infrared spectrogram；Fig. 3 is with amino
Glucose is carbon source biosynthetic Bacterial cellulose infrared spectrogram (corresponding with specific embodiment)；
Fig. 4 is with glucose for carbon source biosynthetic Bacterial cellulose scanning electron microscope (SEM) photograph；
Fig. 5 is with glucose and glucosamine for carbon source biosynthetic Bacterial cellulose scanning electron microscope (SEM) photograph；
Fig. 6 is that it is relative with specific embodiment with glucosamine for carbon source biosynthetic Bacterial cellulose scanning electron microscope (SEM) photograph
Should；
Fig. 7 is the hemolysis rate statistics of the biosynthetic Bacterial cellulose of different carbon source.
Described Bacterial cellulose is to control antibacterial fibre by dosage and the ratio of sugar in regulation acetobacter xylinum cultivating system
Dimension element aperture and physicochemical property.
The hemolysis rate of the Bacterial cellulose of glucose synthesis is 0.38%；The antibacterial of glucose and glucosamine synthesis is fine
The hemolysis rate of dimension element is 0.65%；The hemolysis rate 0.1% of the Bacterial cellulose of glucosamine synthesis.
Detailed description of the invention
Embodiment 1
1, culture medium raw material: 1000mL distilled water, glucose 20 ~ 30g, peptone 7.5 ~ 10g, yeast powder 7.5 ~ 10 g, phosphoric acid
Disodium hydrogen 7.5 ~ 10 g, citric acid 0.5 ~ 1.5 g and pH5.0 ~ 6.0；
2, by above-mentioned raw materials through 121 DEG C of autoclaving 15min, it is cooled to room temperature；
3, being accessed by acetobacter xylinum strain, at 30 DEG C, shaken cultivation 24h becomes acetobacter xylinum seed liquor；
4, dispensing again: 1000mL distilled water, glucose 20 ~ 30g, peptone 7.5 ~ 10 g, yeast powder 7.5 ~ 10 g, phosphoric acid hydrogen
Disodium 7.5 ~ 10 g, citric acid 0.5 ~ 1.5 g and pH5.0 ~ 6.0,121 DEG C of autoclaving 15min, treat that culture medium is cooled to room temperature
After, the ratio that acetobacter xylinum seed liquor is 6% by mass percentage is accessed, at 30 DEG C, quiescent culture obtains Bacterial cellulose in 7 days
Wet film；
5, bacterial cellulose wet-coating is used after flowing water rinses distilled water rinsing；
6, the sodium hydroxide solution of 1% is used to boil 4 hours；
7, soak 0.5 hour in concentration is 0. 5% acetic acid at room temperature with after distilled water flushing；
8, clean with distilled water flushing, it is placed in baking oven and is dried to obtain Bacterial cellulose dry film at 60 DEG C.
Embodiment 2
1000mL distilled water, glucose 20 ~ 30g, peptone 7.5 ~ 10 g, yeast powder 7.5 ~ 10 g, disodium hydrogen phosphate 7.5 ~ 10
G, citric acid 0.5 ~ 1.5 g and pH5.0 ~ 6.0,121 DEG C of autoclaving 15min, after culture medium is cooled to room temperature, by wood vinegar bar
Bacterium strain accesses, and at 30 DEG C, shaken cultivation 24h becomes acetobacter xylinum seed liquor；
1000mL distilled water, glucosamine 5 ~ 7.5g, glucose 15 ~ 25 g, peptone 7.5 ~ 10 g, yeast powder 7.5 ~ 10
G, disodium hydrogen phosphate 7.5 ~ 10 g, citric acid 0.5 ~ 1.5 g and pH5.0 ~ 6.0,121 DEG C of autoclaving 15min, treat that culture medium is cold
But to after room temperature, by acetobacter xylinum seed liquor by mass percentage 6% ratio access, at 30 DEG C, quiescent culture obtains antibacterial in 7 days
Cellulose wet-coating；
Use distilled water rinsing after being rinsed by bacterial cellulose wet-coating flowing water, then use 1% sodium hydroxide solution to boil 4 hours,
Soak 0.5 hour in 0. 5% acetic acid at room temperature with after distilled water flushing again, clean with distilled water flushing；It is placed in baking oven 60
Bacterial cellulose dry film it is dried to obtain at DEG C.
Embodiment 3
1000mL distilled water, glucose 20 ~ 30g, peptone 7.5 ~ 10 g, yeast powder 7.5 ~ 10 g, disodium hydrogen phosphate 7.5 ~ 10
G, citric acid 0.5 ~ 1.5 g and pH5.0 ~ 6.0,121 DEG C of autoclaving 15min, after culture medium is cooled to room temperature, by wood vinegar bar
Bacterium strain accesses, and at 30 DEG C, shaken cultivation 24h becomes acetobacter xylinum seed liquor；
1000mL distilled water, glucosamine 20 ~ 30g, peptone 7.5 ~ 10 g, yeast powder 7.5 ~ 10 g, disodium hydrogen phosphate 7.5
~ 10 g, citric acid 0.5 ~ 1.5 g and pH5.0 ~ 6.0,121 DEG C of autoclaving 15min, after culture medium is cooled to room temperature, by wood
Acetobacter seed liquor is the amount access of 6% by mass percentage, and at 30 DEG C, quiescent culture obtains bacterial cellulose wet-coating in 7 days；
Use distilled water rinsing after being rinsed by bacterial cellulose wet-coating flowing water, then use 1% sodium hydroxide solution to boil 4 hours,
Soak 0.5 hour in 0. 5% acetic acid at room temperature with after distilled water flushing again, clean with distilled water flushing；It is placed in baking oven 60
Bacterial cellulose dry film it is dried to obtain at DEG C.
Claims (5)
1. the preparation method of Blood index Bacterial cellulose, it is characterised in that: by the acetobacter xylinum seed liquor of shaken cultivation 24h
It is in the culture medium of the ratio SH that is inoculated in improvement of 6% by mass percentage, quiescent culture 7 days at 30 DEG C, obtain antibacterial fine
Dimension element wet film；Bacterial cellulose wet-coating uses after flowing water is flushed distilled water rinse, then uses 1% sodium hydroxide solution
Boil 4 hours, then soak half an hour in 0.5% acetic acid at room temperature after flowing water flushing, clean with distilled water flushing, dry.
The most according to claim 1, the preparation method of Blood index Bacterial cellulose, is characterized in that the SH training of described improvement
The preparation method supporting base is: by distilled water 1L, sugar 20 ~ 30g, peptone 7.5 ~ 10 g, yeast powder 7.5 ~ 10 g, disodium hydrogen phosphate
7.5 ~ 10 g, citric acid 0.5 ~ 1.5 g and pH5.0 ~ 6.0 mix homogeneously becomes the SH culture medium of improvement.
The preparation method of Blood index Bacterial cellulose the most according to claim 2, it is characterized in that described sugar be monosaccharide or
The material that polysaccharide or several different carbon source material synthesize in proportion.
The most according to claim 1, the Bacterial cellulose of bioanalysis synthesis, is characterized in that the system of described acetobacter xylinum seed liquor
Preparation Method is: by acetobacter xylinum list colony inoculation in the SH culture medium of improvement, shaken cultivation 24h becomes acetobacter xylinum seed
Liquid.
The most according to claim 1, the preparation method of Blood index Bacterial cellulose, is characterized in that described Bacterial cellulose
It is to control Bacterial cellulose aperture and physicochemical property by dosage and the ratio of sugar in regulation acetobacter xylinum cultivating system.
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| CN201610829248.8A CN106244646A (en) | 2016-09-19 | 2016-09-19 | The preparation method of Blood index Bacterial cellulose |
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| CN201610829248.8A CN106244646A (en) | 2016-09-19 | 2016-09-19 | The preparation method of Blood index Bacterial cellulose |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110872385A (en) * | 2018-08-31 | 2020-03-10 | 南京理工大学 | Preparation method of starch/bacterial cellulose composite material |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101041844A (en) * | 2007-04-29 | 2007-09-26 | 山东轻工业学院 | Method for improving bacteria cellulose output by adding sodium alginate |
| CN101386877A (en) * | 2008-10-30 | 2009-03-18 | 上海应用技术学院 | Method for preparing bacteria cellulose compound film and application thereof as face pack material |
| CN102784071A (en) * | 2012-07-18 | 2012-11-21 | 上海应用技术学院 | Moisturizing eye mask prepared from bacterial cellulose |
-
2016
- 2016-09-19 CN CN201610829248.8A patent/CN106244646A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101041844A (en) * | 2007-04-29 | 2007-09-26 | 山东轻工业学院 | Method for improving bacteria cellulose output by adding sodium alginate |
| CN101386877A (en) * | 2008-10-30 | 2009-03-18 | 上海应用技术学院 | Method for preparing bacteria cellulose compound film and application thereof as face pack material |
| CN102784071A (en) * | 2012-07-18 | 2012-11-21 | 上海应用技术学院 | Moisturizing eye mask prepared from bacterial cellulose |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110872385A (en) * | 2018-08-31 | 2020-03-10 | 南京理工大学 | Preparation method of starch/bacterial cellulose composite material |
| CN110872385B (en) * | 2018-08-31 | 2021-12-10 | 南京理工大学 | Preparation method of starch/bacterial cellulose composite material |
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Application publication date: 20161221 |