CN106244484A - Antarctic krill fungal component, Antarctic krill anti-oxidation peptide and its preparation method and application - Google Patents
Antarctic krill fungal component, Antarctic krill anti-oxidation peptide and its preparation method and application Download PDFInfo
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Abstract
The present invention relates to a kind of Antarctic krill fungal component, Antarctic krill anti-oxidation peptide and its preparation method and application, belong to microorganism and biologically active peptide technical field, Antarctic krill fungal component Psychrobacter NL 6 is preserved in Wuhan, China Type Tissue Collection on June 22nd, 2016, and preserving number is CCTCCM 2016341.It is homogenized with this strain fermentation Antarctic krill, it is thus achieved that a kind of Antarctic krill anti-oxidation peptide, bioactive peptide the component 1～5kDa component that non-oxidizability is the highest, its Scavenging action to hydroxyl free radical reaches 93.0%, and reducing power is 1.32, and ABTS clearance rate is 92.4%.Described polypeptide has preferable inside and outside stability, is suitable for the demand of large-scale production, has preferably exploitation and using value as non-oxidizability food additive.
Description
Technical field
The invention belongs to microorganism and biologically active peptide technical field, relate in particular to a kind of Antarctic krill fungal component,
Antarctic krill anti-oxidation peptide and its preparation method and application.
Background technology
Activity in man's oxygen-derived free radicals (ROS) has certain function, as immunity and signal conductive process, in the increasing of cell
Grow, break up and apoptosis has important function, but too much reactive oxygen free radical just has vandalism.Numerous studies channel syndrome
Bright, the genesis mechanism of aging and aging relevant disorders such as cancers, cardiovascular, autoimmune disease and arthritis etc. with
Interior free yl produces too much or Scavenging ability decline has close relationship.Therefore people cause machine to by free radical
Bulk damage degeneration, the various diseases of generation cause strong attention.Although the antioxidant of synthesis is efficiently, inexpensively, but due to
Having side effect, people more favor in selecting natural antioxidant.Therefore at a low price, efficiently, the opening of the Natural antioxidant of low toxicity
Send out and become study hotspot.Have broad application prospects with aspects such as feed additives at medicine, cosmetics, health product and food.
Microbial fermentation technology, with modern fermentation technique as core, utilizes the metabolic activity process of microorganism, turns through biology
Change and manufacture various industrial fermentation product on a large scale.Fermentation engineering is gentle with its working condition, abundant raw material source and price
Cheap, product is single-minded, and garbage is little to the pollution of environment and easy to handle feature is used widely in many fields.The South Pole
Krill is protein resource maximum on the earth, and in its muscle protein, aminoacid composition is comprehensively, reasonable mixture ratio.Antarctic krill is biological
Rich reserves, Antarctic krill albumen can be as the splendid source of anti-oxidation peptide.Obtain Antarctic krill with microbial fermentation technology to resist
Oxidation peptide, adds the additional value of Antarctic krill, improves the high-valued of Antarctic krill resource and commercial application prospect.
2013, the enzymolysis albumen such as woods Song Yi of Jilin University, and obtain, by technology such as ultrafiltration, the Ovum Gallus domesticus album egg that non-oxidizability is higher
White source antioxidant peptide powder.(publication number: CN 103342734 A)；The Luo Zisheng of Zhejiang University in 2012 etc. are former with prawn Crusta Penaeus seu Panulirus
Material, with hydrolysis by novo, obtains non-oxidizability the least molecule anti-oxidation peptide (publication number: CN 102676623 A).
Summary of the invention
The technical problem to be solved in the present invention be to provide a kind of Antarctic krill fungal component, Antarctic krill anti-oxidation peptide and
Preparation method and application, Psychrobacter NL-6 is a kind of Antarctic krill fungal component screened from Antarctic krill, uses this bacterial strain
Fermentation Antarctic krill homogenate, it is thus achieved that a kind of Antarctic krill anti-oxidation peptide, it has broad application prospects as food additive.
One strain Antarctic krill fungal component Psychrobacter NL-6 (Psychrobacter sp.NL-6), this Psychrobacter NL-6
Being preserved in China typical culture collection center on June 22nd, 2016, preserving number is CCTCC M 2016341.
Further, 16S rDNA length 1439bp of described Psychrobacter NL-6, sequence is shown in SEQ NO.1.
Further, described Psychrobacter NL-6 is gram negative bacteria, and colony characteristics is white or milk yellow, surface light
Sliding, there are projection, neat in edge, scanning electron microscopic picture shows, it is bar-shaped that bacterial strain NL-6 presents quarter butt, and phage surface is smooth, and part is just
The thalline of division presents ellipsoid shape, and major part thalline is in binary fission state, and two thalline are connected, and intermediate recess, junction
Elongated, there is mucosa to produce between thalline, the size of single thalline is: wide: 0.6-0.9 μm, long: 0.8-1.5 μm.
The present invention also provides for a kind of Antarctic krill anti-oxidation peptide, and described Antarctic krill anti-oxidation peptide is by Psychrobacter NL-6
Fermentation Antarctic krill homogenate obtains, and its step is as follows:
1) strain culturing:
Take Psychrobacter NL-6 bacterial strain, be inoculated in 2216E fluid medium, 25-37 DEG C of condition, 150-220r/min,
Fermentation culture 8-14h；
Bacterial strain after above-mentioned cultivation is inoculated in the shaking flask containing 2216E fluid medium with the inoculum concentration of volume ratio 4%,
25-37 DEG C of condition, 150-220r/min, fermentation culture 8-14h, obtain seed fermentation liquid；
2) Antarctic krill cold water thaws, and breaks into homogenate with refiner, by mass volume ratio 8-15% wet weight distilled water
Dissolve, 121 DEG C of autoclaving 20min, prepare Antarctic krill culture medium；
3) by step 1) the seed fermentation liquid for preparing is inoculated in step 2 with volume ratio 6%-18% inoculum concentration) south prepared
In the krill culture medium of pole, 25-37 DEG C, 150-220r/min, fermentation culture 24-48h, obtain strain fermentating liquid；
4) by step 3) the fermentation liquid 10000r/min for preparing is centrifuged 30min, collects supernatant, with molecular weight be 1kDa,
The ultrafilter membrane ultrafiltration of 5kDa and 10kDa, obtains each component of<1kDa, 1～5kDa, 5～10kDa,>10kDa, by cold for each component
Lyophilizing is dry, obtains antioxidant peptide powder.
Further, described Antarctic krill anti-oxidation peptide is preferably 1～5kDa.
The present invention also provides for a kind of described Antarctic krill anti-oxidation peptide answering in food, food additive and health product
With.
The present invention also provides for the application in cosmetics of a kind of 1～5kDa described Antarctic krill anti-oxidation peptide.
Present invention beneficial effect compared with prior art:
The present invention uses microbial fermentation technology to obtain anti-oxidation peptide, and technique is simple, and with low cost, abundant raw material is easy to get,
Reaction condition is gentle, and particularly this bacterial strain is to screen the new bacterium that a kind of Psychrobacter obtained belongs to from Antarctic krill fungal component,
There is the homology of bacterium source and culture medium, there is preferable ferment effect, it is to avoid use expensive enzyme reagent, saved one-tenth
This.The anti-oxidation peptide vitro stability prepared is preferable, is suitable for the demand of large-scale production；And internal stability is preferable, makees
For non-oxidizability food additive, there is preferably exploitation and using value.
Owing to Oil repellent higher in Antarctic krill is the main cause affecting its application, to Antarctic krill of the present invention
Before and after anti-oxidation peptide fermentation, in lyophilized powder, Oil repellent contrast finds, Oil repellent (327 ± 3.4mg kg in the lyophilized powder after fermentation-1) compared to (3407 ± 2.5mg kg before fermentation-1) reduce 10 times more than, and less than EU criteria (350mg kg-1), therefore
Antarctic krill anti-oxidation peptide after fermentation is directly as egg albumen powder, or the additive as food, health product all has preferably
Application prospect.
The non-oxidizability of each component of Antarctic krill anti-oxidation peptide has preferable stability in vitro, and at high temperature, (100 DEG C are boiled
Under the conditions of 1h), each component Scavenging activity on hydroxyl free radical is all positively retained at more than 98%, reducing power be all positively retained at 84% with
On, wherein 1～5kDa and 5～10kDa components reducing power under each pH (2,4,6,8 and 10) is all positively retained at more than 96%；
Therefore by < anti-oxidation peptide of 5kDa as cosmetics additive, there is good stability, molecular weight is little, be prone to the advantage that absorbs.
The each component of Antarctic krill anti-oxidation peptide is to there being Ca2+Under conditions of existence, the equal energy of Scavenging action to hydroxyl free radical, reducing power
It is maintained at more than 90%, therefore by < anti-oxidation peptide of 5kDa can be with all kinds of calcium series products with clothes, its antioxidation as food additive
Performance is barely affected.
Antarctic krill anti-oxidation peptide each component non-oxidizability is with Pepsase-Trypsin-Chymotrypsin compound enzyme
Having preferable stability in the internal gastrointestinal tract environment of system simulation, 1～5kDa component Scavenging activity on hydroxyl free radical is through gastric environment
After simulation, activity is maintained at 98.6%, and after gastroenteric environment is simulated, activity is maintained at 84.7%；Reducing power is simulated through gastric environment
Rear activity is maintained at 96.7%, and after gastroenteric environment is simulated, activity is maintained at 82.0%, further demonstrates 1～5kDa little point
Son amount anti-oxidation peptide has preferable inside and outside stability, has preferable using value as food additive.
Accompanying drawing explanation
The scanning electron microscope (SEM) photograph of Fig. 1 Psychrobacter NL-6；
The phylogenetic tree of Fig. 2 Psychrobacter NL-6；
Psychrobacter NL-6, Latin is Psychrobacter sp.NL-6, is preserved in China on June 22nd, 2016
Type Tissue Collection, preserving number is CCTCC M 2016341, and preservation address is Wuhan, China Wuhan University.
Detailed description of the invention
Further illustrate technical scheme below by embodiment, but protection scope of the present invention is not implemented
Any type of restriction of example.
The screening of embodiment 1 bacterial strain NL-6
1, has the acquisition of antioxidant activity fermentation liquid
1) Antarctic krill is cleaned by the screening of symbiotic microorganism bacterial strain, mortar grinder, takes 1g grinding and is homogenized in aseptic distillation
In water, 150-220r/min, vibrate 1～2h, take suspension in distilled water, gradient dilution, coat 2216E solid medium
On, cultivate 24-48h for 25-37 DEG C, the different 10 strain bacterium (numbering NL-(1～10)) of picking mode of appearance are in 2216E liquid culture
Base is cultivated, preserves strain.
2) bacterial strain activation culture:
10 strain symbiosis bacterial strains are inoculated in the test tube of 2216E fluid medium, 25-37 DEG C of condition, 150-220r/min,
Fermentation culture 8-14h.
Bacterial strain after above-mentioned cultivation is inoculated in the shaking flask containing 2216E fluid medium with the inoculum concentration of volume ratio 4%,
25-37 DEG C of condition, 150-220r/min, fermentation culture 8-14h, obtain seed fermentation liquid.
3) Antarctic krill cold water thaws, and breaks into homogenate with refiner, by mass volume ratio 8-15% (wet weight) distillation
Water dissolution, 121 DEG C of autoclaving 20min, prepare Antarctic krill culture medium.
4) prepared seed fermentation liquid is inoculated in Antarctic krill culture medium with volume ratio 6%-18% inoculum concentration, 25-
37 DEG C, 150-220r/min, fermentation culture 24-48h, obtain strain fermentating liquid.
5) strain fermentating liquid 10000r/min is centrifuged 30min, takes supernatant.
2, non-oxidizability measures
1) Scavenging action to hydroxyl free radical measures
In 10mL test tube, add the liquid to be measured of 2mL, be then sequentially added into the FeSO that 2mL concentration is 9mmol/L4, 2mL is dense
Degree is the salicylic acid of 9mmol/L, is eventually adding the H that 2mL concentration is 9mmol/L2O2Start reaction, in 510nm after standing 10min
Place measures absorbance A1.In view of the light absorption value of extracting solution itself, do sample blank, measure the absorbance A of deduction sample blank2,
Measure the absorbance A of blank liquid simultaneously0。
Clearance rate (%)=(A0-A1+A2)/A0×100
2) reducing power measures
Add each supernatant 2.5mL of step 1 preparation, be simultaneously introduced phosphate buffer (0.2mol/L, the pH of 2.5mL
6.6) and the potassium ferricyanide solution of 2.5mL 1%.The trichloroacetic acid of 2.5mL 10% is added after 50 DEG C of water-bath 20min of mixture,
Room temperature stands 10min.Take 2.5mL reactant liquor, add 2.5mL distilled water and 0.5mL0.1% ferric chloride solution.After reaction 10min
Measuring light absorption value at 700nm, the reproducibility of this value the highest explanation sample is the strongest.
Table 1 10 strain symbiotic microorganism fermentation liquid non-oxidizability size compares
| Bacterial strain | Scavenging action to hydroxyl free radical | Reducing power |
| NL-1 | 89.8% | 0.487 |
| NL-2 | 78.4% | 0.270 |
| NL-3 | 79.5% | 0.388 |
| NL-4 | 85.4% | 0.441 |
| NL-5 | 87.8% | 0.453 |
| NL-6 | 91.7% | 0.604 |
| NL-7 | 74.3% | 0.414 |
| NL-8 | 68.3% | 0.484 |
| NL-9 | 75.9% | 0.440 |
| NL-10 | 81.4% | 0.375 |
By table 1, we have chosen Scavenging action to hydroxyl free radical and reducing power all preferably bacterial strain NL-6 grinds further
Study carefully, and bacterial strain NL-6 has been carried out strain identification.
Embodiment 2
The strain identification of bacterial strain NL-6
(1) morphological characteristic
Gram’s staining result shows, bacterial strain NL-6 is gram negative bacteria, and colony characteristics is white or milk yellow, surface
Smooth, there are projection, neat in edge.Scanning electron microscopic picture shows, it is bar-shaped that bacterial strain NL-6 presents quarter butt, and phage surface is smooth, part
Just the thalline of division presents ellipsoid shape, and major part thalline is in binary fission state, and two thalline are connected, and intermediate recess, connect
Locate elongated, have mucosa to produce between thalline, see Fig. 1.The size that can substantially be calculated single thalline by Fig. 1 is: wide: 0.6-0.9 μm,
Long: 0.8-1.5 μm.
(2) biochemical reactions feature
Owing to Gram’s staining shows, this bacterium is gram negative bacteria, therefore can carry out by API 20E Bacteria Identification system
Physiological and biochemical analysis.The results are shown in Table 2
The Physiology and biochemistry qualification result of table 2 bacterial strain NL-6
Note: "-" table is negative；"+", represents the positive
Glucose, rhamnose, melibiose, arabinose is utilized it is recognised that bacterial strain NL-6 can ferment by this table.
(3) 16S rDNA sequence analysis
The bacterial strain NL-6 bacterium solution being in exponential phase of absorption 1.5mL, 4 DEG C, 10000r/min, centrifugal 10min.Abandon
Clear liquid, adds the TE buffer of 400 μ L, with rifle head by precipitation piping and druming uniformly, adds the lysozyme of 10 μ L, mix homogeneously, is placed in
90min in 37 DEG C of water-baths, 4 DEG C, 10000r/min, centrifugal 10min, retain supernatant and be DNA solution.
Use 16S rDNA universal primer, by Qingdao Hua Da gene chemical synthesis.Forward primer: 27F 5 '-
AGAGTTTGATCCTGGTCAG-3 ', reverse primer: 1492R 5 '-CGGCTACCTTGTTACGACTT-3 '；16S rDNA sequence
PCR condition be: 95 DEG C of denaturations 5min；95 DEG C of degeneration 30s, 55 DEG C of annealing 30s, 72 DEG C extend 1min, circulate 30 times, 72 DEG C
Extend 10min.PCR reaction system is: reaction system: 50 μ L, template DNA: 2 μ L, forward primer: 2 μ L, downstream primer: 2 μ L,
2X Easy tap MasterMix:25 μ L, distilled water: 19 μ L.
Sepharose electrophoresis with 1% detects pcr amplification product, compares for standard molecular weight with D2000Marker, about exists
There is a pcr amplification product band at 1500bp, the 16S rDNA PCR primer of bacterial strain NL-6 is delivered to Qingdao Hua Da gene and enters
Row order-checking.Sequence is shown in SEQ NO.1, and the 16S rDNA sequence length recording bacterial strain NL-6 is 1439bp.
The 16S rDNA sequence of bacterial strain NL-6 is analyzed by BLAST in the nucleic acid database of NCBI.With
BioEdit software carries out multiple sequences alignments, the bacterial strain high with 16S rDNA sequence (1439bp) similarity system design of bacterial strain NL-6
For Psychrobacter fozii NF23 (T) (98.82%), Psychrobacter okhotskensis MD17 (T)
(98.47%), Psychrobacter cryohalolentis K5 (T) (98.33%).Download and experimental strain sibship
Nearer sequence, is drawn by MEGA6 software Neighbor-joining option and grows tree such as Fig. 2.
By " common bacteria system identification handbook ", it may be determined that this bacterial strain belongs to Psychrobacter and belongs to
(Psychrobacter) antibacterial, named Psychrobacter sp.NL-6, the novel species that may belong to for Psychrobacter.
The preparation of embodiment 3 anti-oxidation peptide
1) activation of bacterial strain
Antarctic krill fungal component Psychrobacter NL-6 is inoculated in the test tube of 2216E fluid medium, 28 DEG C, 200r/
Min, cultivates 10h；Bacterial strain after above-mentioned cultivation is inoculated in the shaking flask containing fermentation medium with 4% inoculum concentration (volume ratio),
28 DEG C of conditions, 200r/min, fermentation culture 10h.Obtain seed fermentation liquid.
2) NL-6 fermentation Antarctic krill
Antarctic krill cold water thaws, and breaks into homogenate with refiner, water-soluble with distillation by mass volume ratio 10% (wet weight)
Solve, 121 DEG C of autoclaving 20min, prepare Antarctic krill culture medium.
Prepared seed fermentation liquid is inoculated in Antarctic krill culture medium with volume ratio 8% inoculum concentration, 32 DEG C, 200r/
Min, fermentation culture 48h, obtain strain fermentation suspension.
3) prepared by each component anti-oxidation peptide
Prepared fermentation liquid 10000r/min is centrifuged 30min, collects supernatant, with molecular weight be 1kDa,
The ultrafilter membrane ultrafiltration of 5kDa, 10kDa, obtains each of<1kd, 1～5kDa, 5～10kDa,>10kDa
Component, by each component lyophilization, obtains antioxidant peptide powder.
Empirical tests, the bioactive peptide component that the non-oxidizability of gained of the present invention is the highest is 1～5kDa component, its
Scavenging action to hydroxyl free radical reaches 93.0%, and reducing power is 1.32, and ABTS clearance rate is 92.4%.
Embodiment 4 in vitro anti-oxidation Stability Determination
Temperature conditions measures
Each component that embodiment 3 is obtained respectively at 20 DEG C, 40 DEG C, 60 DEG C, 80 DEG C, water bath with thermostatic control 1h under the conditions of 100 DEG C,
Its Scavenging action to hydroxyl free radical, reducing power size is surveyed after placing room temperature.It is experimentally confirmed that each component Scavenging activity on hydroxyl free radical is maintained at
More than 98%, reducing power is maintained at more than 84%.
PH condition measures
Each component embodiment 3 obtained is dissolved separately in the buffer that pH is 2,4,6,8 and 10, surveys its reducing power big
Little.It is experimentally confirmed that 1～5kDa and 5～10kDa component reducing powers under each pH are all positively retained at more than 96%；
Ca2+Impact
Each component embodiment 3 obtained is dissolved in the Ca that concentration is 5mmol/L2+In solution, survey its antioxidant activity shadow
Ring.It is experimentally confirmed that each component is to there being Ca2+Under conditions of existence, Scavenging action to hydroxyl free radical, reducing power all can be maintained at 90% with
On, therefore by < food additive that the anti-oxidation peptide of 5kDa prepares, molecular weight is little, it is easy to absorb, and can be same with all kinds of calcium series products
Clothes, its antioxygenic property is barely affected.
Non-oxidizability Stability Determination in embodiment 5 body
Using Pepsase-Trypsin-Chymotrypsin compound enzyme system, Antarctic krill is resisted by Imitative gastroenteric environments
Oxidation peptide activity influence.
1) preparation of pepsin enzyme liquid
Configuration pH is the potassium chloride-hydrochloride buffer of 2.0, stand-by.Accurately weigh 1.00g Pepsase and be dissolved in 100mL
The simulated gastric fluid of configuration, now with the current.
2) preparation of simulated intestinal fluid takes 50mL pepsin enzymolysis solution, and it is 7.0 that the NaOH solution of configuration 2.0M regulates its pH,
Add Trypsin and Chymotrypsin (1:1) of 1% (w/w).
3) the digestive environments analytical balance in simulation stomach weighs 1.00g Antarctic krill polypeptide lyophilized powder (P5 component), molten
In the pepsin enzymolysis solution (37 ± 1) DEG C of 50mL, vibrate under the conditions of 100r/min 2h, is drawn off, boils in boiling water bath
10min, makes pepsin inactivate, and terminates reaction, is cooled to room temperature, and with 2M NaOH regulation pH value to 7.0.Take 25mL digestion
Liquid, in the centrifuge 30min of 12000r/min, is collected clear liquid, is measured its antioxidant activity.
4) digestive environments in simulation intestinal takes out the sample 25mL having digested 2h in pepsin enzymolysis solution, regulates PH
Being 7.0, add simulated intestinal fluid 25mL, in (37 ± 1) DEG C, vibrate under the conditions of 100r/min 2h, is drawn off, boils in boiling water bath
Boiling 10min so that it is the protease inactivation contained, terminates reaction, is cooled to room temperature, and centrifugal, regulation rotating speed is 12000r/min, from
Heart 30min, and detect the free radical scavenging activity of supernatant.
Verified, each component non-oxidizability is being simulated with Pepsase-Trypsin-Chymotrypsin compound enzyme system
Having preferable stability in gastrointestinal tract environment, 1～5kDa component Scavenging activity on hydroxyl free radical activity after gastric environment is simulated keeps
98.6%, after gastroenteric environment is simulated, activity is maintained at 84.7%；Reducing power activity after gastric environment is simulated is maintained at
96.7%, after gastroenteric environment is simulated, activity is maintained at 82.0%.
Claims (9)
1. a strain Antarctic krill fungal component Psychrobacter NL-6, it is characterised in that this Psychrobacter NL-6 was on June 22nd, 2016
Being preserved in China typical culture collection center, preserving number is CCTCC M 2016341.
A strain Antarctic krill fungal component Psychrobacter NL-6 the most according to claim 1, it is characterised in that described addicted to cold bar
16S rDNA length 1439bp of bacterium NL-6, sequence is shown in SEQ NO.1.
A strain Antarctic krill fungal component Psychrobacter NL-6 the most according to claim 1, it is characterised in that described addicted to cold bar
Bacterium NL-6 is gram negative bacteria, and colony characteristics is white or milk yellow, smooth surface, has projection, neat in edge, scanning electron microscope
Picture shows, it is bar-shaped that bacterial strain NL-6 presents quarter butt, and phage surface is smooth, and the thalline of part just division presents ellipsoid shape, major part
Thalline is in binary fission state, and two thalline are connected, and intermediate recess, and junction is elongated, has mucosa to produce between thalline, single bacterium
The size of body is: wide: 0.6-0.9 μm, long: 0.8-1.5 μm.
4. an Antarctic krill anti-oxidation peptide, it is characterised in that described Antarctic krill anti-oxidation peptide is any by claim 1-3
One described Psychrobacter NL-6 fermentation Antarctic krill homogenate obtains, and its step is as follows:
1) strain culturing:
Take Psychrobacter NL-6 bacterial strain, be inoculated in 2216E fluid medium, 25-37 DEG C of condition, 150-220r/min, fermentation
Cultivate 8-14h；
Bacterial strain after above-mentioned cultivation is inoculated in the shaking flask containing 2216E fluid medium with the inoculum concentration of volume ratio 4%, 25-
37 DEG C of conditions, 150-220r/min, fermentation culture 8-14h, obtain seed fermentation liquid；
2) Antarctic krill cold water thaws, and breaks into homogenate with refiner, dissolves by mass volume ratio 8-15% wet weight distilled water,
121 DEG C of autoclaving 20min, prepare Antarctic krill culture medium；
3) by step 1) the seed fermentation liquid for preparing is inoculated in step 2 with volume ratio 6%-18% inoculum concentration) South Pole phosphorus prepared
In shrimp culture medium, 25-37 DEG C, 150-220r/min, fermentation culture 24-48h, obtain strain fermentating liquid；
4) by step 3) the fermentation liquid 10000r/min for preparing is centrifuged 30min, collects supernatant, with molecular weight be 1kDa, 5kDa and
The ultrafilter membrane ultrafiltration of 10kDa, obtains each component of<1kDa, 1～5kDa, 5～10kDa,>10kDa, by each component lyophilization,
Obtain Antarctic krill antioxidant peptide powder.
A kind of Antarctic krill anti-oxidation peptide the most according to claim 4, it is characterised in that described Antarctic krill antioxidation
Peptide is 1～5kDa.
6. the application in food, food additive and health product of the Antarctic krill anti-oxidation peptide described in a claim 4.
7. contain food or the health product of Antarctic krill anti-oxidation peptide described in claim 4.
8. the application in cosmetics of the Antarctic krill anti-oxidation peptide described in a claim 5.
9. contain the cosmetics of Antarctic krill anti-oxidation peptide described in claim 5.
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| CN108130352A (en) * | 2017-12-06 | 2018-06-08 | 华南协同创新研究院 | A kind of method for preparing krill anti-oxidation peptide and the anti-oxidation peptide and application |
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