CN106244436B - A kind of microorganism fungus kind card punch - Google Patents

A kind of microorganism fungus kind card punch Download PDF

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Publication number
CN106244436B
CN106244436B CN201610865109.0A CN201610865109A CN106244436B CN 106244436 B CN106244436 B CN 106244436B CN 201610865109 A CN201610865109 A CN 201610865109A CN 106244436 B CN106244436 B CN 106244436B
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CN
China
Prior art keywords
fixed
rod
strut
card punch
bucket body
Prior art date
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Expired - Fee Related
Application number
CN201610865109.0A
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Chinese (zh)
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CN106244436A (en
Inventor
吴力卡
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
B Enjoy Biotechnology Co ltd
Original Assignee
Guangzhou Biologic Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangzhou Biologic Biotechnology Co Ltd filed Critical Guangzhou Biologic Biotechnology Co Ltd
Priority to CN201810574601.1A priority Critical patent/CN108728341B/en
Priority to CN201610865109.0A priority patent/CN106244436B/en
Publication of CN106244436A publication Critical patent/CN106244436A/en
Application granted granted Critical
Publication of CN106244436B publication Critical patent/CN106244436B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus

Abstract

The invention discloses a kind of microorganism fungus kind card punch, including taper bucket body, oblique holding rod is fixed with taper bucket body, the bottom of taper bucket body is hinged with bottom cover, and bottom cover is fixed on the lifting rod of arc, and the bottom of taper bucket body forms guide ring, the inserting groove of arc is formed on bottom cover, inserting groove is plugged on guide ring, and guide ring upper sleeve has the reduction torsion spring of annular, and one end of reduction torsion spring is fixed on guide ring, the other end is fixed on the side wall of inserting groove;Supporting rod is formed on oblique holding rod, the lower end of supporting rod forms guide groove, guide pad is plugged with guide groove, the upper end of guide pad is fixed with strut, lower end is fixed with connecting rod, connecting rod is fixedly connected on one end of lifting rod, strut upper sleeve has spring, and tooth-strip part is formed on the side wall of strut, and tooth-strip part engagement is connected with adjustment gear.Fungus block is taken out in the convenient punching of the present invention, and fungus block easily departs from from card punch when fungus block is not easy to slide and be inoculated with from card punch when mobile.

Description

A kind of microorganism fungus kind card punch
Technical field:
The present invention relates to the technical field of microbial cultivation device, is specifically related to a kind of microorganism fungus kind card punch.
Background technology:
In production, scientific experiment in microorganism, strain is accessed into plating medium, treats that strain is grown into the medium After certain area, become tablet containing bacterium.At this time, commonly use card punch takes fungus block of the same size (culture) to connect from tablet containing bacterium Enter in fresh culture medium or gel.At present, fungus block easily slides from card punch, connects when conventional use of card punch has mobile The shortcomings that fungus block is not easy to depart from from card punch during kind, and service speed is slow, and work efficiency is low, and in punching and inoculation, culture Ware lid opens excessive or overlong time, be easy to cause living contaminants.
The content of the invention:
The purpose of the present invention aims to solve the problem that problem existing in the prior art, there is provided a kind of to be not easy to make culture dish pollution microbes In the case of convenient punching take out fungus block, fungus block takes off easily from card punch when fungus block is not easy to slide and be inoculated with from card punch when mobile From microorganism fungus kind card punch.
The present invention relates to a kind of microorganism fungus kind card punch, including taper bucket body, the upper end lateral wall of the taper bucket body On be fixedly connected with oblique holding rod, the bottom of taper bucket body is hinged with bottom cover, and one end both sides of the bottom cover are cut with scissors by articulated shaft It is connected in taper bucket body bottom sidewall, on the one end for the lifting rod that the bottom of the other end is fixed on arc, between the articulated shaft Taper bucket body bottom sidewall on form guide ring, the inserting groove of arc is formed on bottom cover, the inserting groove is plugged on On the guide ring, guide ring upper sleeve has the reduction torsion spring of annular, and one end of the reduction torsion spring is fixed on guide ring, is another One end is fixed on the side wall of inserting groove;
It is described tiltedly to form the tilting supporting rod at an acute angle with oblique holding rod on holding rod, the lower end of the supporting rod into Type has guide groove, is plugged with guide pad in the guide groove, and the upper end of the guide pad is fixed with strut, lower end is fixed with connection Bar, the connecting rod is fixedly connected on one end of the lifting rod through escape groove tilting on the oblique holding rod, described The upper end of strut passes through locating piece, and the locating piece is fixed on the upper end of supporting rod, and strut upper sleeve has spring, the spring Both ends are pressed up against on locating piece and guide pad, and tooth-strip part is formed on the side wall of strut, and a part for the tooth-strip part is inserted Be connected in spring, another part through locating piece and engages and is connected with adjustment gear, the adjustment gear is hinged on supporting rod.
By above-mentioned technical proposal, when in use, operating personnel hold oblique holding rod and stir tune card punch of the invention Section gear causes adjustment gear to rotate, and adjustment gear drives the tooth-strip part that is engaged with to move up, tooth-strip part drive strut to Upper movement, strut, which moves up, drives guide pad to be moved up in the guide groove of supporting rod, and spring is compressed, and strut, which drives, to be connected Extension bar also moves up, and connecting rod drives the lifting rod of hinged arc on it to move up, one end band mantle of lifting rod The bottom cover of shape bucket body bottom is rotated around with taper bucket body hinged place, and the inserting groove on bottom cover turns around guide ring in the process Move and compress reduction torsion spring, so that the bottom opening of taper bucket body, it is real then to push the taper bucket body on tablet containing bacterium Existing fungus block punching, above carries taper bucket body after completing punching and unclamps adjustment gear, under the double action of spring and reduction torsion spring, Connecting rod and lifting rod reset, and drive bottom cover to reset rotation around with taper bucket body hinged place, reduction torsion spring resets so that grafting Groove rotates on guide ring, until bottom cover is covered in the bottom of taper bucket body again, so that fungus block will not be from taper bucket body Inside drop out, then taper bucket body is moved to above fresh culture medium or gel, by inverting oblique holding rod by taper bucket body It is inverted, so as to fungus block be moved in fresh culture medium or gel.
Through the above scheme, microorganism fungus kind card punch of the invention is not easy to make to facilitate in the case of culture dish pollution microbes Fungus block is taken out in punching, and fungus block easily departs from from card punch when fungus block is not easy to slide and be inoculated with from card punch when mobile.
Preferably, the upper end of the supporting rod forms hinged groove to one kind as such scheme, the hinged groove it is front and rear Articulated shaft is fixed with side wall, for the middle part sleeve of the adjustment gear on the articulated shaft, one end of adjustment gear is pierced by hinge Access slot is engaged with the tooth-strip part of strut, the other end is pierced by hinged groove and is fixed with the protective case of sector, the side wall of the protective case On form protrusion stir key.
One kind as such scheme preferably, forms two limited blocks, described two limited blocks point on the guide pad It is not resisted against in the two lateral walls of supporting rod.
Preferably, the strut is round bar to one kind as such scheme, and strut is parallel with supporting rod, the central shaft of strut Line and the central axis of connecting rod are located along the same line.
One kind as such scheme is preferably fixedly connected with reinforcing rib between the supporting rod and oblique holding rod.
One kind as such scheme preferably forms bullet on the top surface of one end that the bottom cover is fixedly connected with lifting rod Property cushion.
Preferably, the rear end of the tiltedly holding rod is fixed with handle to one kind as such scheme, oblique on front side of the handle Block is fixed with holding rod.
Described above is only the general introduction of technical solution of the present invention, in order to better understand the technological means of the present invention, And can be practiced according to the content of specification, below with presently preferred embodiments of the present invention and coordinate attached drawing describe in detail as after.
Brief description of the drawings:
The following drawings is only intended to, in doing schematic illustration and explanation to the present invention, not delimit the scope of the invention.Wherein:
Fig. 1 is the structure diagram of the present invention;
Fig. 2 is the partial structural diagram of Fig. 1 top views;
Fig. 3 is the partial sectional view of Fig. 1;
Fig. 4 is another partial sectional view of Fig. 1;
Fig. 5 is the working state schematic representation of Fig. 4.
Embodiment:
With reference to the accompanying drawings and examples, the embodiment of the present invention is described in further detail.Implement below Example is used to illustrate the present invention, but is not limited to the scope of the present invention.
Referring to Fig. 1, Fig. 2, Fig. 4, a kind of microorganism fungus kind card punch of the present invention, including taper bucket body 10, it is described Oblique holding rod 20 is fixedly connected with the upper end lateral wall of taper bucket body, the rear end of the tiltedly holding rod is fixed with handle 21, institute State and block 22 is fixed with the oblique holding rod 20 on front side of handle, the bottom of taper bucket body 10 is hinged with bottom cover 30, the bottom cover One end both sides are hinged in 10 bottom sidewall of taper bucket body by articulated shaft 11, the bottom of the other end is fixed on the lifting rod of arc On 40 one end, elastic cord breaker 31 is formed on the top surface of one end that bottom cover 30 is fixedly connected with lifting rod 40, it is described hinged Guide ring 12 is formed in the bottom sidewall of taper bucket body 10 between axis 11, the inserting groove 32 of arc is formed on bottom cover 30, The inserting groove is plugged on 12 on the guide ring, and guide ring upper sleeve has the reduction torsion spring 50 of annular, the reduction torsion spring One end is fixed on guide ring 12, the other end is fixed on the side wall of inserting groove 32.
It is described tiltedly to form the tilting supporting rod 60 at an acute angle with oblique holding rod 20 on holding rod 20 referring to Fig. 1, Fig. 3, Reinforcing rib 23 is fixedly connected between the supporting rod and oblique holding rod 20, the lower end of supporting rod 60 forms guide groove 61, institute State and guide pad 70 is plugged with guide groove, two limited blocks 71 are formed on the guide pad, described two limited blocks support respectively In the two lateral walls for leaning against supporting rod 60, the upper end of guide pad 70 is fixed with strut 80, lower end is fixed with connecting rod 90, described Connecting rod is fixedly connected on one end of the lifting rod 40 through escape groove 24 tilting on the oblique holding rod 20, the branch Bar 80 is round bar, and strut 80 is parallel with supporting rod 60, and the central axis of strut 80 is with the central axis of connecting rod 90 positioned at same On one straight line, the upper end of strut 80 passes through locating piece 62, and the locating piece is fixed on the upper end of supporting rod 60,80 upper sleeve of strut There is spring 100, the both ends of the spring press up against on locating piece 62 and guide pad 70, are molded on the side wall of strut 80 with teeth Bar portion 81, the part of the tooth-strip part is plugged in spring 100, another part through locating piece 62 and engages and is connected with adjusting Gear 200, the adjustment gear are hinged on supporting rod 60.
Referring to Fig. 3, the upper end of the supporting rod 60 forms hinged groove 63, is fixed with the front-rear side walls of the hinged groove Articulated shaft 64, on the articulated shaft 64, one end of adjustment gear 200 is pierced by be hinged the middle part sleeve of the adjustment gear 200 Groove 63 is engaged with the tooth-strip part 81 of strut 80, the other end is pierced by hinged groove 63 and is fixed with the protective case 300 of sector, the protection Protrusion is formed on the side wall of set stirs key 301.
Referring to Fig. 5, in the specific implementation, operating personnel hold oblique holding rod 20 and stir adjusting tooth card punch of the invention Wheel 200 is so that adjustment gear 200 rotates, and adjustment gear 200 drives the tooth-strip part 81 being engaged with to move up, 81 band of tooth-strip part Dynamic strut 80 moves up, and strut 80, which moves up, drives guide pad 70 to be moved up in the guide groove 61 of supporting rod 60, bullet Spring 100 is compressed, and strut 80 drives connecting rod 90 to also move up, and connecting rod 90 drives the lifting rod of hinged arc on it 40 move up, and one end of lifting rod 40 drives the bottom cover 30 of 10 bottom of taper bucket body to turn around with 10 hinged place of taper bucket body Dynamic, the inserting groove 32 on bottom cover 30 rotates around guide ring 12 and compresses reduction torsion spring 50 in the process, so that cone The bottom opening of shape bucket body 10, the taper bucket body 10 is then pushed on tablet containing bacterium and realizes that fungus block is punched, is above carried after completing punching Taper bucket body 10 simultaneously unclamps adjustment gear 200, under the double action of spring 100 and reduction torsion spring 50, connecting rod 90 and lifting Bar 40 resets, and drives bottom cover 30 to reset rotation around with 10 hinged place of taper bucket body, reduction torsion spring 50 resets so that inserting groove 32 Rotated on guide ring 12, until bottom cover 30 is covered in the bottom of taper bucket body 10 again, so that fungus block will not be from taper Drop out, then taper bucket body 10 is moved to above fresh culture medium or gel, by inverting oblique holding rod 20 in bucket body 10 Taper bucket body 10 is inverted, so as to fungus block be moved in fresh culture medium or gel.
In conclusion the microorganism fungus kind card punch of the present invention is not easy to make conveniently to punch in the case of culture dish pollution microbes Fungus block is taken out, fungus block easily departs from from card punch when fungus block is not easy to slide and be inoculated with from card punch when mobile.
The embodiment of microorganism fungus kind card punch provided by the present invention, the only present invention, but the guarantor of the present invention Shield scope be not limited thereto, any one skilled in the art the invention discloses technical scope in, can be light It is readily conceivable that change or replacement, should all covers within the scope of the present invention.Therefore, protection scope of the present invention should be with the power Subject to the protection domain that profit requires.

Claims (7)

  1. A kind of 1. microorganism fungus kind card punch, it is characterised in that:
    Including taper bucket body (10), oblique holding rod (20), the taper bucket are fixedly connected with the upper end lateral wall of the taper bucket body The bottom of body (10) is hinged with bottom cover (30), and one end both sides of the bottom cover are hinged on taper bucket body (10) by articulated shaft (11) In bottom sidewall, the bottom of the other end be fixed on one end of lifting rod (40) of arc, the cone between the articulated shaft (11) Guide ring (12) is formed in the bottom sidewall of shape bucket body (10), the inserting groove (32) of arc is formed on bottom cover (30), it is described Inserting groove is plugged on the guide ring (12), and guide ring (12) upper sleeve has the reduction torsion spring (50) of annular, and described reset is turned round One end of spring is fixed on guide ring (12), the other end is fixed on the side wall of inserting groove (32);
    It is described tiltedly to form the tilting supporting rod (60) at an acute angle with oblique holding rod (20), the supporting rod on holding rod (20) Lower end form guide groove (61), guide pad (70) is plugged with the guide groove, the upper end of the guide pad is fixed with branch Bar (80), lower end are fixed with connecting rod (90), and the connecting rod passes through escape groove (24) tilting on the oblique holding rod (20) It is fixedly connected on one end of the lifting rod (40), the upper end of the strut (80) passes through locating piece (62), the locating piece The upper end of supporting rod (60) is fixed on, strut (80) upper sleeve has spring (100), and the both ends of the spring press up against positioning On block (62) and guide pad (70), tooth-strip part (81), a part of grafting of the tooth-strip part are formed on the side wall of strut (80) In spring (100), another part passes through locating piece (62) and engagement is connected with adjustment gear (200), the adjustment gear hinge It is connected on supporting rod (60).
  2. 2. microorganism fungus kind card punch according to claim 1, it is characterised in that:The upper end shaping of the supporting rod (60) There is hinged groove (63), articulated shaft (64) is fixed with the front-rear side walls of the hinged groove, the middle part of the adjustment gear (200) is inserted It is sleeved on the articulated shaft (64), one end of adjustment gear (200) is pierced by the tooth-strip part (81) of hinged groove (63) and strut (80) Engagement, the other end are pierced by hinged groove (63) and are fixed with the protective case (300) of sector, are formed on the side wall of the protective case convex What is gone out stirs key (301).
  3. 3. microorganism fungus kind card punch according to claim 1, it is characterised in that:Two are formed on the guide pad (70) A limited block (71), described two limited blocks are born against in the two lateral walls of supporting rod (60).
  4. 4. microorganism fungus kind card punch according to claim 1, it is characterised in that:The strut (80) is round bar, strut (80) parallel with supporting rod (60), the central axis of strut (80) is located at same straight line with the central axis of connecting rod (90) On.
  5. 5. microorganism fungus kind card punch according to claim 1, it is characterised in that:The supporting rod (60) and oblique holding rod (20) reinforcing rib (23) is fixedly connected between.
  6. 6. microorganism fungus kind card punch according to claim 1, it is characterised in that:The bottom cover is fixedly connected with lifting rod One end top surface on form elastic cord breaker.
  7. 7. microorganism fungus kind card punch according to claim 1, it is characterised in that:The rear end of the tiltedly holding rod (20) is consolidated Surely there is a handle (21), block (22) is fixed with the oblique holding rod (20) on front side of the handle.
CN201610865109.0A 2016-09-25 2016-09-25 A kind of microorganism fungus kind card punch Expired - Fee Related CN106244436B (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201810574601.1A CN108728341B (en) 2016-09-25 2016-09-25 Puncher for microbial strains
CN201610865109.0A CN106244436B (en) 2016-09-25 2016-09-25 A kind of microorganism fungus kind card punch

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610865109.0A CN106244436B (en) 2016-09-25 2016-09-25 A kind of microorganism fungus kind card punch

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201810574601.1A Division CN108728341B (en) 2016-09-25 2016-09-25 Puncher for microbial strains

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CN106244436A CN106244436A (en) 2016-12-21
CN106244436B true CN106244436B (en) 2018-04-27

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CN201610865109.0A Expired - Fee Related CN106244436B (en) 2016-09-25 2016-09-25 A kind of microorganism fungus kind card punch
CN201810574601.1A Active CN108728341B (en) 2016-09-25 2016-09-25 Puncher for microbial strains

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109370888A (en) * 2018-11-17 2019-02-22 济宁医学院 The dedicated harvester of mycobacterium tuberculosis bacterium colony

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US4179339A (en) * 1977-03-02 1979-12-18 Olympus Optical Company, Ltd. Liquid feeder for automatic culture apparatus
IL112842A (en) * 1995-03-02 1998-06-15 Batec Bio Analytical Techn Ltd Device for detecting microorganisms in a sample
ES2126483B1 (en) * 1996-04-23 1999-11-16 Iul S A BIOLOGICAL SAMPLE INOCULATION SYSTEM IN THE AGAR SURFACE IN PETRI CAPSULES.
CN202374741U (en) * 2011-12-31 2012-08-15 佳木斯大学 Bacteria piece inoculator
CN202455778U (en) * 2012-03-21 2012-10-03 北京农业职业学院 Cultivation substrate puncher
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CN104439519A (en) * 2014-11-14 2015-03-25 成都市宏山科技有限公司 High-pressure punching mechanism
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CN108728341A (en) 2018-11-02
CN108728341B (en) 2021-12-31
CN106244436A (en) 2016-12-21

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Inventor after: Wu Lika

Inventor before: Li Fenglang

Inventor before: Li Shuxin

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Effective date of registration: 20180330

Address after: 511400 Guangzhou City, Guangzhou, Guangdong, Shibi Road, shiik, Panyu District Stone Wall Street, one of the Shishi highway, one of the No. 95 201

Applicant after: B-ENJOY BIOTECHNOLOGY CO.,LTD.

Address before: 523000 Guangdong province Dongguan City Songshan Lake high tech Industrial Zone Building 406 industrial development productivity

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