CN106244416B - Cell microinjection chip, injection device and its working method based on dielectrophoresis - Google Patents
Cell microinjection chip, injection device and its working method based on dielectrophoresis Download PDFInfo
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- CN106244416B CN106244416B CN201610615682.6A CN201610615682A CN106244416B CN 106244416 B CN106244416 B CN 106244416B CN 201610615682 A CN201610615682 A CN 201610615682A CN 106244416 B CN106244416 B CN 106244416B
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- 238000004720 dielectrophoresis Methods 0.000 title abstract description 10
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- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
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Abstract
The present invention relates to a kind of cell microinjection chip, injection device and its working method based on dielectrophoresis, this cell microinjection chip include：Fluid channel and multistage fluid channel are injected, wherein the multistage fluid channel is suitable for screening cell, so that only one cell enters in injection fluid channel, carries out microinjection；Cell microinjection chip, injection device and its working method of the present invention, to generate corresponding dielectrophoretic force using corresponding microelectrode array, such as Conventional dielectric swimming power, traveling wave dielectrophoretic force and rotation dielectrophoretic force, to control respectively, cell is mobile, rises or falls, pose adjustment, improve traditional microinjection chip by micro syringe pump drive the micro-fluid movement in it in the way of, reduce equipment complexity and cost, this cell microinjection chip can improve the efficiency of microinjection, easy to utilize.
Description
Technical field
The invention belongs to RESEARCH ON CELL-BIOLOGY technical field, specifically a kind of experiment for cell microinjection fills
It sets.
Background technology
Microinjection technique is based on intracellular microinjection and Micro-perfusion in Graft After technology, in more and more experimental biologies
Become a very universal microoperation technology in research field, such as in vitro in fertilization, transgenosis etc..These operations are
Pass through single or multiple tubular glass micropipette, accurate positioning device (micromanipulator) and micro syringe
Or microperfusion device carries out on single cell.Microinjection is micro- using the glass of tip superfine (0.1 to 5 μm)
Injection needle is measured, exogenous genetic fragment is injected directly into protokaryon phase embryo or the cell of culture, then by host genome sequence
Arrange may occur recombination, missing, duplication or transposition phenomena such as and make foreign gene insertion host chromosome in.It is this micro-
The program for injecting art need to have quite accurate micromanipulator, and when manufacturing long tip, device, injection need to be elongated with micropipet
When need to have the micromanipulation device of fixed tip position.The strong point of this technology is any DNA can be passed to any kind in principle
Class it is intracellular.Compared with other transgenic technologys, the advantages of microinjection be transgene efficiency is stable, injection gene size not
Be restricted, without carrier and operation object without particular/special requirement etc., animals on the brink of extinction protection, reproduction and health, species improvement and
It is used widely in intracellular caryoplasm research.This method has successfully applied to include mouse, fish, rat, rabbit and many large sizes
Domestic animal, such as ox, sheep, pig Transgenic animals.
Micropipette used in these operations draws needle device to make with capillary, first adds capillary glass tube
The temperature that heat is melted to it, then it is drawn into the diameter and taper of required suitable size, the diameter of micropipette microcephaly
(as low as 0.2 micron) is related to the high-precision of fiber executor, it can be used for accurately liquid relief.This accuracy can be each
It is accurate, repeated good in the sub- picoliters grade of the cell of type and arbitrary size offer or 0.1 micron of (micron) range
Intracellular and cell sidenote penetrate.By using direct hydraulic pressure (pressure injection) or not by using flow, and by applying
Add an electric field to make charged ion move (iontherapy), can obtain to squeeze the substance in micropipette and spray
Effect.
The disadvantage is that equipment is accurate and expensive, the prolonged practice of operating technology needs, and can only inject every time limited
Cell.Moreover, conventional cell microinjection device, before carrying out microinjection to cell, there is no be adjusted to cell
It is suitble to " posture " of injection, this will substantially reduce the survival rate of cell after microinjection.Although presently, there are adjustment cell appearances
The device of state, but its by cell pose adjustment it is good after, have can for microinjection device and the device of adjustment cell posture when injection
It can interfere or uncoordinated and influence microinjection effect.It is necessary to propose further solution party in view of the above problems
Case.
Invention content
The object of the present invention is to provide a kind of cell microinjection chip, injection device and its working methods, are situated between with passing through
Electrophoresis power drive cell is moved to injection fluid channel, to complete to inject.
In order to solve the above technical problem, the present invention provides a kind of cell microinjection chips, including：Inject fluid channel
With multistage fluid channel, wherein it is described multistage fluid channel be suitable for cell is screened so that only one cell enters injection miniflow
In road, microinjection is carried out.
Further, the multistage fluid channel includes：At least two-stage fluid channel；That is level-one fluid channel and two level fluid channel, and
The width of flow path of next stage fluid channel is less than the width of flow path of upper level fluid channel；And the bottom of the level-one fluid channel is equipped with
First microelectrode array, first microelectrode array are suitable for driving cell after powered up and suspend and moved to two level fluid channel；Institute
The bottom for stating two level fluid channel is equipped with the second microelectrode array；After cell enters two level fluid channel from level-one fluid channel, pass through
Second microelectrode array adjustment cell enters the hoverheight and movement speed of injection fluid channel.
Further, the bottom of the injection fluid channel is equipped with third microelectrode array, and top is equipped with the 4th microelectrode array,
And the 4th microelectrode array center at be equipped with injection needle；The third microelectrode array is suitable for control cell to micro-
The underface of injection needle is moved, and captures cell by the 4th microelectrode array, it is made to be positioned at injection needle just
Lower section；And by third, the 4th microelectrode array synergic adjustment cell posture after, so that cell is moved up or down；Thin
When born of the same parents move up, the microinjection is needled into cell, is injected；When cell moves down, cell detachment microinjection has been injected
Needle, and cell will have been injected by third microelectrode array and be discharged into output fluid channel.
Further, the bottom of the output fluid channel is equipped with the 5th microelectrode array；And it is powered in the 5th microelectrode array
Afterwards, make to have injected cell output.
Further, the junction of the upper level fluid channel and lower two level fluid channel is in horn mouth transition.
Further, the injection fluid channel, multistage fluid channel and output fluid channel are located in miniflow channel layer, and
The upper and lower of the miniflow channel layer has been covered each by quartz substrate, and described first, second, third and the 5th microelectrode
Array distribution is distributed in upper layer quartz substrate in lower layer's quartz substrate, the 4th microelectrode array.
Further, described first, third and the 5th microelectrode array are formed by parallel pole arranged at equal intervals, and parallel
Electrode is orthogonal with the moving direction of cell；Second microelectrode array is connect using two fork-shaped electrode fingers；And described
Each electrode extends in the spaced 90 ° of angles of circumferencial direction, and to the central axis of injection needle in four microelectrode arrays.
Another aspect, the present invention also provides a kind of cell microinjection devices.
The cell microinjection device includes：Control for connecting each microelectrode array in cell microinjection chip
Module；The control module generates the corresponding control signal of each microelectrode array, is moved to from multistage fluid channel with controlling cell
Injection fluid channel will inject cell from injection needle to inject cellular localization, pose adjustment, and after the completion of injection
It is exported after disengaging.
The third aspect, the present invention also provides a kind of working methods of cell microinjection device.
The working method of the cell microinjection device includes the following steps：
Step S1, screens cell, and only one cell is made to enter in injection fluid channel；Step S2, it is micro- to entering to inject
Cell in runner is injected；And step S3, cell output will have been injected.
Further, what the cell microinjection chip used in the cell microinjection device was suitable for using is described thin
Born of the same parents' microinjection chip.
The invention has the advantages that cell microinjection chip, injection device and its working method of the present invention, to adopt
Corresponding dielectrophoretic force, such as Conventional dielectric swimming power, traveling wave dielectrophoretic force and rotation dielectrophoretic force are generated with corresponding microelectrode array,
To control cell movement respectively, rise or fall, pose adjustment, improves traditional microinjection chip and driven using micro syringe pump
The mode of micro-fluid movement in it, reducing equipment complexity and cost, this cell microinjection chip can improve micro-
The efficiency of injection, it is easy to utilize.
Description of the drawings
Present invention will be further explained below with reference to the attached drawings and examples.
Fig. 1 is the overall structure diagram of the microinjection chip of the present invention；
Fig. 2 be the present invention miniflow channel layer in injection fluid channel, multistage fluid channel and the structural schematic diagram for exporting fluid channel；
Fig. 3 is the main section of the microinjection chip of the present invention；
Fig. 4 is the arrangement schematic diagram of each electrode in each microelectrode array in lower layer's quartz substrate；
Fig. 5 be the present invention upper layer quartz substrate in electrode structure schematic diagram；
Fig. 6 is operating diagram of the cell of the present invention in injection fluid channel.
In figure：
Lower layer's quartz substrate 1, miniflow channel layer 2, upper layer quartz substrate 3, PDMS blocks 4, injection needle 5, cell 6；
Level-one fluid channel 201, two level fluid channel 202, injection fluid channel 203, output fluid channel 204；
First microelectrode array 11, the second microelectrode array 12, third microelectrode array 13, the 4th microelectrode array 14,
5th microelectrode array 15；
Each parallel pole 1101,1102,1103,1104,1105 and 1106 in first microelectrode array；
Two fork-shaped electrode 1201,1202 in second microelectrode array；
Each parallel pole 1301,1302,1303,1304 in third microelectrode array；
Each electrode 1401,1402,1403,1404 in 4th microelectrode array；
Each electrode 1501 in 5th microelectrode array, 1502,1503,1504,1505,1506,1507,1508,1509,
1510。
Specific implementation mode
In conjunction with the accompanying drawings, the present invention is further explained in detail.These attached drawings are simplified schematic diagram, only with
Illustration illustrates the basic structure of the present invention, therefore it only shows the composition relevant to the invention.
Since current microinjection device does not all adjust its position before carrying out microinjection to cell, table is studied
Bright egg mother cell first polar body is located at different location in intracytoplasmic sperm injection, sends out cell survival rate, Fertilized quality and embryo
It educates and has a significant impact.Usual operating position of the requirement of experiment first polar body far from injection needle, to reduce to cells such as spindles
The injury of device.And this cell microinjection chip, cell microinjection device and working method are carrying out micro- note to cell
Its posture can be adjusted before penetrating, this by greatly improve carry out micromanipulation after cell survival rate.
Term is explained：
Dielectrophoresis：Also referred to as dielectrophoresis is neutral particle stress and the phenomenon that move in non-uniform electric field.Including often
Advise dielectrophoresis, traveling wave dielectrophoresis and rotation dielectrophoresis.
Dimethyl silicone polymer：(Polydimethylsioxane) abbreviation PDMS is a kind of the macromolecule organic silicon chemical combination
Object.With optical clear, and under normal circumstances, it is considered to be inertia, it is nontoxic, it is nonflammable, there is good bio-compatibility.
Embodiment 1
As shown in figures 1 and 3, cell microinjection chip includes four parts, i.e., what is stacked gradually from bottom to top contains phase
Answer lower layer's quartz substrate 1 of microelectrode array, miniflow channel layer 2, the upper layer quartz substrate 3 containing microelectrode array, microinjection
The PDMS blocks 4 of needle 5 and fixed injection needle；Injection needle 5 and PDMS blocks 4 are by microinjection needle handle and PDMS blocks
Aperture interference fit realize sealing.
Specifically, cell microinjection chip includes：Fluid channel 203 and multistage fluid channel are injected, wherein the multistage is micro-
Runner is suitable for screening cell, so that only one cell 6 enters in injection fluid channel 203, cell 6 is hit microinjection
The needle point of needle 5 to carry out microinjection after puncturing cell again；And the needle point of injection needle 5 passes through the center of upper layer quartz substrate
Hole is stretched into injection fluid channel 203.
As shown in Fig. 2, the multistage fluid channel includes：At least two-stage fluid channel；That is level-one fluid channel 201 and two level miniflow
Road 202, and the width of flow path of next stage fluid channel is less than the width of flow path of upper level fluid channel；And the level-one fluid channel
Bottom be equipped with the first microelectrode array 11, first microelectrode array 11 be suitable for after powered up driving cell suspend and it is micro- to two level
Runner moves；The bottom of the two level fluid channel is equipped with the second microelectrode array 12；When cell enters two level from level-one fluid channel
After fluid channel, the hoverheight and movement speed that cell enters injection fluid channel are adjusted by the second microelectrode array.
The width of flow path of two level fluid channel is more than the width of flow path of injection fluid channel, the width of flow path of the injection fluid channel
More than cell dia to be injected, and less than 2 times of cell dia to be injected.
The bottom of the injection fluid channel is equipped with third microelectrode array 13, and top is equipped with the 4th microelectrode array 14, with
And the 4th microelectrode array center at be equipped with the injection needle 5；The third microelectrode array be suitable for control cell to
The underface of injection needle is moved, and captures cell by the 4th microelectrode array, it is made to be positioned at injection needle
Underface；And by third, the 4th microelectrode array synergic adjustment cell posture after, so that cell is moved up or down；
When cell moves up, the microinjection is needled into cell, is injected；When cell moves down, it is micro- cell detachment has been injected
Injection needle, and cell will have been injected by third microelectrode array and be discharged into output fluid channel 204.
The bottom of the output fluid channel 204 is equipped with the 5th microelectrode array 15；And it is powered in the 5th microelectrode array 15
Afterwards, make to have injected cell output.
The junction of the upper level fluid channel and lower two level fluid channel is in horn mouth transition.
The injection fluid channel, multistage fluid channel and output fluid channel are respectively positioned in miniflow channel layer, described first, second,
Third and the 5th microelectrode array are distributed in lower layer's quartz substrate, and the 4th microelectrode array is distributed in upper layer quartz substrate.
As shown in Figure 4 and Figure 5, described first, third and the 5th microelectrode array are by parallel strip electrodes arranged at equal intervals
It forms, and parallel strip electrodes are orthogonal with the moving direction of cell；Second microelectrode array connect using two fork-shaped electrode fingers and
At, and it is in be spacedly distributed to refer to receiving electrode；And in the 4th microelectrode array in each above quartz substrate center of electrode
Centered on heart hole, circumferential array obtains, i.e., 90 ° each other of each electrode, and extends to the central axis of injection needle.
It is changed in the proportional region that fluid channel and the size of electrode can be certain according to the size of cell to be injected.
The related implementation steps of this cell microinjection chip will be discussed in detail in embodiment 3.
Embodiment 2
On the basis of embodiment 1, the present embodiment 2 additionally provides a kind of cell microinjection device.
The cell microinjection device includes：Control for connecting each microelectrode array in cell microinjection chip
Module；The control module generates the corresponding control signal of each microelectrode array, is moved to from multistage fluid channel with controlling cell
Injection fluid channel will inject cell from injection needle to inject cellular localization, pose adjustment, and after the completion of injection
It is exported after disengaging.
Involved cell microinjection chip is as described in Example 1 in the present embodiment 2.
Embodiment 3
On the basis of embodiment 1 and embodiment 2, the present embodiment 3 additionally provides a kind of work of cell microinjection device
Method.
The working method of the cell microinjection device, includes the following steps：
Step S1, screens cell, and only one cell is made to enter in injection fluid channel；
Step S2 is injected to entering the cell injected in fluid channel；And
Step S3 will inject cell output.
The cell microinjection device is suitable for use cell microinjection device as described in Example 2 and this is thin
The cell microinjection as described in Example 1 that cell microinjection chip used in born of the same parents' microinjection device is suitable for using
Chip.
Cell is screened in the step S1, only one cell is made to enter the specific method packet in injection fluid channel
It includes：Suspension cell solution to be injected is injected from level-one fluid channel in chip with pipettor；In cell microinjection device
Control module is suitable for using the first microelectrode array of multichannel sine wave signal generator pair that can generate frequency and phase-adjustable
In after each parallel pole (1101,1102,1103,1104,1105 and 1106) applies the sinusoidal signal that phase differs 90 degree successively,
So that level-one fluid channel 201 in generate inhomogeneous field so that its inner cell can simultaneously by Conventional dielectric swimming power and
(the Conventional dielectric swimming field of force makes cell be suspended in fluid channel to traveling wave dielectrophoretic force, is made cellular water by the traveling wave dielectrophoresis field of force
It is flat to move downward)；Horn mouth transition region is moved under the action of traveling wave dielectrophoretic force of the cell in level-one fluid channel 201
It is interior, stop the phase change of the sinusoidal signal on the first microelectrode array, reduces the mobile speed that cell enters two level fluid channel 202
Degree, plays the effect of buffering；Again at the second microelectrode array of 202 bottom of two level fluid channel (two fork-shaped electrodes 1201,1202)
Upper application sinusoidal signal makes to adjust the hoverheight of cell under the action of dielectrophoretic force of the cell in two level fluid channel and to it
Slow down, i.e., cell buffers in the area under the action of motional inertia, and reduces the speed for being moved to injection fluid channel 203.
Include to entering the method that the cell injected in fluid channel is injected in the step S2：
The traveling wave dielectrophoresis that parallel pole (1301,1302,1303 and 1304) induction in third microelectrode array generates
Power can make the rotation forward or backward of cell 6, while continue to move horizontally；The sense of rotation of cell 6 is referring to Fig. 6.It is positive corresponding with it
Top the 4th microelectrode array in each electrode (1401,1402,1403 and 1404) dielectric for generating under Conventional dielectric swimming
Swimming power can be always in by attracting and capturing cell 6 (its force trapping F illustrates referring to the citing in Fig. 6) to control cell 6
The underface of microinjection needle；The Conventional dielectric of traveling wave dielectrophoretic force and the 4th microelectrode array that third microelectrode array generates is swum
The coupling of both power can be with manipulation cell posture and motion state, i.e.,：The underface for hovering over microinjection needle 5, may make
Cell spins and vertical up and down motion；The cell microinjection device is suitable for movement tendency according to cell at this time, and with it is pre-
The cell movement direction and goal position versus of phase carries out corresponding reaction type adjusting to signal frequency and voltage, that is, passes through control
The frequency and phase of its dielectrophoretic force generated of the signal frequency and voltage indirect control of signal generator generation processed, it is thin to make
The spatial attitude of born of the same parents in the area is adjusted；Specifically, each electrode in the 4th microelectrode array on upper layer quartz substrate 3
(1401,1402,1403 and 1404) generate non-uniform electric field, such as, but not limited to the phase for applying signal be 0 °, 180 °,
At 0 °, 180 °, the Conventional dielectric swimming power of induction can control cell straight up or move downward；Have continuously applying signal
When phase (such as 0 °, 90 °, 180 °, 270 °) of equal difference, the rotation dielectrophoretic force of generation is induced to can control cell in the horizontal plane
Rotation.After cell pose adjustment is good, the pattern and frequency of sinusoidal signal, make it lure in adjusting third, the 4th microelectrode array
Artificial delivery life Conventional dielectric swimming power makes cell move straight up, after cell is pierced by microinjection needle 5, by being connected with microinjection needle
Accurate micro syringe pump by allogenic material solution to be injected inject cell interior, cell complete microinjection after, adjust third, the 4th
The frequency of sinusoidal signal on microelectrode array, the Conventional dielectric swimming power that induction generates make to have injected cell move straight down and
Be detached from microinjection needle, then adjust in third microelectrode array four parallel poles (1301,1302,1303 and 1304) generate it is non-even
Highfield induces the cell that the traveling wave dielectrophoretic force of generation can control completion microinjection to move in the horizontal direction to output miniflow
Road 204.
Include by the method for having injected cell output in the step S3：
After cell enters fluid channel 204, adjust each electrode in the 5th microelectrode array (1501,1502,1503,1504,
1505,1506,1507,1508,1509 and 1510) on the phase and frequency of AC signal (apply phase and differ 90 degree
Sinusoidal signal, and adjust the frequency of sinusoidal signal), so that the cell of completion microinjection is moved under the action of traveling wave dielectrophoretic force
Export the output port of fluid channel 204.And so on, you can complete the microinjection operation of remaining cell in fluid channel.
Optionally, listed by the quantity of respective electrode is such as, but not limited in embodiment 1 to embodiment 3 in each microelectrode array
The quantity of act, such as 8 or 12 or 16 differed.
It is enlightenment with above-mentioned desirable embodiment according to the present invention, through the above description, relevant staff is complete
Various changes and amendments can be carried out without departing from the scope of the technological thought of the present invention' entirely.The technology of this invention
Property range is not limited to the contents of the specification, it is necessary to determine its technical scope according to right.
Claims (4)
1. a kind of cell microinjection chip, which is characterized in that including：
Fluid channel and multistage fluid channel are injected, wherein
The multistage fluid channel is suitable for screening cell, so that only one cell enters in injection fluid channel, carries out micro-
Injection；
It is described multistage fluid channel include：At least two-stage fluid channel；I.e.
Level-one fluid channel and two level fluid channel, and the width of flow path of next stage fluid channel is wide less than the runner of upper level fluid channel
Degree；And
The bottom of the level-one fluid channel is equipped with the first microelectrode array, and it is thin which is suitable for driving after powered up
Born of the same parents suspend and are moved to two level fluid channel；
The bottom of the two level fluid channel is equipped with the second microelectrode array；
After cell enters two level fluid channel from level-one fluid channel, cell is adjusted by the second microelectrode array and enters injection miniflow
The hoverheight and movement speed in road；
The bottom of the injection fluid channel is equipped with third microelectrode array, and top is micro- equipped with the 4th microelectrode array and the 4th
Injection needle is equipped at the center of electrod-array；
The third microelectrode array is suitable for control cell and is moved to the underface of injection needle, and passes through the 4th micro- electricity
Pole array captures cell, it is made to be positioned at the underface of injection needle；And
After third, the 4th microelectrode array synergic adjustment cell posture, cell is made to move up or down；
When cell moves up, the microinjection is needled into cell, is injected；
When cell moves down, cell detachment injection needle is injected, and be discharged into cell has been injected by third microelectrode array
Export fluid channel；
The bottom of the output fluid channel is equipped with the 5th microelectrode array；And
After the energization of the 5th microelectrode array, make to have injected cell output；
The injection fluid channel, multistage fluid channel and output fluid channel are located in miniflow channel layer, and
The upper and lower of the miniflow channel layer has been covered each by quartz substrate, and described first, second, third and the 5th microelectrode array
It is distributed in lower layer's quartz substrate, the 4th microelectrode array is distributed in upper layer quartz substrate；
Described first, third and the 5th microelectrode array are formed by parallel pole arranged at equal intervals, and
Parallel pole is orthogonal with the moving direction of cell；
Second microelectrode array is connect using two fork-shaped electrode fingers；And
Each electrode is in the spaced 90 ° of angles of circumferencial direction in 4th microelectrode array, and to the center of injection needle
Extend at axis.
2. cell microinjection chip according to claim 1, which is characterized in that the upper level fluid channel and lower two level
The junction of fluid channel is in horn mouth transition.
3. a kind of cell microinjection device, which is characterized in that including：
Control module for connecting each microelectrode array in cell microinjection chip as claimed in claim 1 or 2；
The control module generates the corresponding control signal of each microelectrode array, and note is moved to from multistage fluid channel to control cell
Fluid channel is penetrated to inject cellular localization, pose adjustment, and will inject cell after the completion of injection and taken off from injection needle
From rear output.
4. a kind of working method of cell microinjection device, includes the following steps：
Step S1, screens cell, and only one cell is made to enter in injection fluid channel；
Step S2 is injected to entering the cell injected in fluid channel；And
Step S3 will inject cell output；
Cell microinjection chip used in the cell microinjection device is suitable for using as claimed in claim 1 or 2
Cell microinjection chip.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610615682.6A CN106244416B (en) | 2016-07-29 | 2016-07-29 | Cell microinjection chip, injection device and its working method based on dielectrophoresis |
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| CN201610615682.6A CN106244416B (en) | 2016-07-29 | 2016-07-29 | Cell microinjection chip, injection device and its working method based on dielectrophoresis |
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| CN106244416B true CN106244416B (en) | 2018-10-23 |
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| CN108359577B (en) * | 2018-01-29 | 2022-03-04 | 燕山大学 | Zebra fish egg rotation micro-operation system based on micro-fluid drive and control method |
| CN109706054B (en) * | 2019-01-04 | 2019-10-22 | 哈尔滨工业大学 | The automatic conveying of zebra fish juvenile fish and the micro runner device of adjustment posture |
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| CN104140927A (en) * | 2014-07-31 | 2014-11-12 | 苏州大学 | Cell position and attitude adjusting chip, device and method |
| CN105441325B (en) * | 2015-10-20 | 2017-12-19 | 河海大学常州校区 | Microinjection chip, control device and the method for work of adjustable ganglion cell's posture |
| CN105483002B (en) * | 2016-01-27 | 2018-06-29 | 河海大学常州校区 | Controlled doses microinjection device and its operating method |
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