CN106239944A - A kind of method that biological adhesiveness is quickly improved on laser patterning PDMS surface - Google Patents
A kind of method that biological adhesiveness is quickly improved on laser patterning PDMS surface Download PDFInfo
- Publication number
- CN106239944A CN106239944A CN201610753010.1A CN201610753010A CN106239944A CN 106239944 A CN106239944 A CN 106239944A CN 201610753010 A CN201610753010 A CN 201610753010A CN 106239944 A CN106239944 A CN 106239944A
- Authority
- CN
- China
- Prior art keywords
- pdms
- pdms surface
- laser
- high energy
- laser beam
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000004205 dimethyl polysiloxane Substances 0.000 title claims abstract description 122
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 title claims abstract description 122
- 238000000034 method Methods 0.000 title claims abstract description 44
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 title claims abstract description 27
- 238000000059 patterning Methods 0.000 title claims abstract description 25
- 235000013870 dimethyl polysiloxane Nutrition 0.000 title claims abstract 25
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 title claims abstract 25
- 230000009471 action Effects 0.000 claims abstract description 24
- 230000012010 growth Effects 0.000 claims abstract description 11
- 238000007385 chemical modification Methods 0.000 claims abstract description 9
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 8
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 8
- 102000004190 Enzymes Human genes 0.000 claims abstract description 5
- 108090000790 Enzymes Proteins 0.000 claims abstract description 5
- 230000010261 cell growth Effects 0.000 claims abstract description 5
- 239000003102 growth factor Substances 0.000 claims abstract description 5
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 5
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 5
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 5
- 238000009834 vaporization Methods 0.000 claims abstract description 5
- 230000008016 vaporization Effects 0.000 claims abstract description 5
- 230000033001 locomotion Effects 0.000 claims description 14
- 230000004048 modification Effects 0.000 abstract description 19
- 238000012986 modification Methods 0.000 abstract description 19
- 239000000463 material Substances 0.000 abstract description 14
- 238000002360 preparation method Methods 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 31
- 230000000694 effects Effects 0.000 description 10
- 230000008569 process Effects 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 8
- 230000021164 cell adhesion Effects 0.000 description 7
- 238000010521 absorption reaction Methods 0.000 description 6
- 229920001223 polyethylene glycol Polymers 0.000 description 6
- 235000018102 proteins Nutrition 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 5
- 238000001259 photo etching Methods 0.000 description 5
- 238000006116 polymerization reaction Methods 0.000 description 5
- 238000001179 sorption measurement Methods 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- 108010039918 Polylysine Proteins 0.000 description 4
- 230000004913 activation Effects 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- -1 polydimethylsiloxane Polymers 0.000 description 4
- 229920000656 polylysine Polymers 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000000975 bioactive effect Effects 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 230000000875 corresponding effect Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000009832 plasma treatment Methods 0.000 description 3
- 230000005855 radiation Effects 0.000 description 3
- 238000002444 silanisation Methods 0.000 description 3
- KDXKERNSBIXSRK-RXMQYKEDSA-N D-lysine Chemical compound NCCCC[C@@H](N)C(O)=O KDXKERNSBIXSRK-RXMQYKEDSA-N 0.000 description 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000007334 copolymerization reaction Methods 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 125000005442 diisocyanate group Chemical group 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 125000000524 functional group Chemical group 0.000 description 2
- 229960002897 heparin Drugs 0.000 description 2
- 229920000669 heparin Polymers 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000007711 solidification Methods 0.000 description 2
- 230000008023 solidification Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 description 1
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- ZNZYKNKBJPZETN-WELNAUFTSA-N Dialdehyde 11678 Chemical compound N1C2=CC=CC=C2C2=C1[C@H](C[C@H](/C(=C/O)C(=O)OC)[C@@H](C=C)C=O)NCC2 ZNZYKNKBJPZETN-WELNAUFTSA-N 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- VQTUBCCKSQIDNK-UHFFFAOYSA-N Isobutene Chemical compound CC(C)=C VQTUBCCKSQIDNK-UHFFFAOYSA-N 0.000 description 1
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 229910002808 Si–O–Si Inorganic materials 0.000 description 1
- 238000007259 addition reaction Methods 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 230000001427 coherent effect Effects 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 239000007822 coupling agent Substances 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000006880 cross-coupling reaction Methods 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 239000003822 epoxy resin Substances 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000007792 gaseous phase Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000006459 hydrosilylation reaction Methods 0.000 description 1
- 150000003949 imides Chemical class 0.000 description 1
- 239000003999 initiator Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000007641 inkjet printing Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000002715 modification method Methods 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 229920002946 poly[2-(methacryloxy)ethyl phosphorylcholine] polymer Polymers 0.000 description 1
- 229920001690 polydopamine Polymers 0.000 description 1
- 229920000867 polyelectrolyte Polymers 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
- 229920000151 polyglycol Polymers 0.000 description 1
- 239000010695 polyglycol Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- KRIOVPPHQSLHCZ-UHFFFAOYSA-N propiophenone Chemical compound CCC(=O)C1=CC=CC=C1 KRIOVPPHQSLHCZ-UHFFFAOYSA-N 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000001338 self-assembly Methods 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- RSPCKAHMRANGJZ-UHFFFAOYSA-N thiohydroxylamine Chemical compound SN RSPCKAHMRANGJZ-UHFFFAOYSA-N 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C71/00—After-treatment of articles without altering their shape; Apparatus therefor
- B29C71/04—After-treatment of articles without altering their shape; Apparatus therefor by wave energy or particle radiation, e.g. for curing or vulcanising preformed articles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C59/00—Surface shaping of articles, e.g. embossing; Apparatus therefor
- B29C59/16—Surface shaping of articles, e.g. embossing; Apparatus therefor by wave energy or particle radiation, e.g. infrared heating
Abstract
The invention discloses a kind of method that biological adhesiveness is quickly improved on laser patterning PDMS surface, micro structure can be formed by the PDMS surface after laser action, provide a good environment for cell attachment growth；Or by the PDMS surface after laser action, chemical modification or structurally-modified occurs, thus improve biological adhesiveness；Described micro structure can be with fixing protein, cell growth factor, enzyme and nucleic acid；Laser instrument produces high energy pulse laser beam, described high energy pulse laser beam is after reflecting mirror, it is focused to PDMS surface by convex lens, under described high energy pulse laser beam radiates the high-energy and impact force action brought, there is photothermy and photochemical action in PDMS surface, and instant vaporization or splash, thus form micro structure on PDMS surface.The present invention utilizes laser action material surface modifying or forms surface micro-structure, in the case of not affecting non-modified region surface, simplifies PDMS surface modification step, patterns PDMS surface, be greatly improved preparation efficiency.
Description
Technical field
The present invention relates to a kind of biological adhesiveness technology improving PDMS surface, particularly relate to a kind of laser patterning PDMS
The method of biological adhesiveness is quickly improved on surface, belongs to laser surface modification field.
Background technology
PDMS (polydimethylsiloxane), have good easy injection moldability, bio-compatibility, light transmission, breathability,
Heat stability and the characteristic such as nontoxic, be used not only for the micro-fluidic chip that processing is conventional, increasingly also is used as spatial cell and cultivates chip
Structural material.But, the PDMS easy non-specific adsorption protein of non-modified process or little hydrophobic molecule, it is unfavorable for thin
The adhesion of born of the same parents and propagation.So needing PDMS is carried out surface modification, could preferably be used as cell surface attachment cultivate and
The research of cell proliferation.
At present, biomolecule modification is a kind of means of PDMS surface modification.The biomolecule of material surface is modified and is referred to
On the basis of molecular biology, utilize various surface modification method by biologies such as protein, cell growth factor, enzyme and nucleic acid
Molecule is fixed on the molecular biosciences material needed for the surface of certain material, structure, specifically identifies and detects corresponding molecule.
The material that biomolecule is modified, while improving the biocompatibility of material, with the addition of again new biological activity, has extensively
Application prospect.
The surface modifying method that PDMS biomolecule is modified mainly is changed by physical absorption, surface active and chemical graft
Property and the method that both combine realize.Physical absorption includes nonionic surfactant, polyelectrolyte absorption etc.；Table
Face activation method includes the radiation of Cement Composite Treated by Plasma, UV ozone, chemical gaseous phase deposition and LBL self-assembly etc., grafting modification
Mainly include that plasma grafting, UV surface cause polymerization, body grafting modification, silanization, Si-H addition reaction, biology to live
Property molecular modification etc..
Physical absorption: physical absorption or coating are one of relatively simple convenient surface modifying methods, it generally by
The polymer or copolymer with biocompatibility are adsorbed at material surface by the effect such as intermolecular hydrogen bond, ionic charge, from
And improve the biocompatibility of material surface.If PDMS surface adsorption is with the PLL (polylysine) of a large amount of cationes, Ke Yiyou
Effect ground promotes that surface is with the mammalian cell adhesion of positive charge and growth.
Surface active: by plasma treatment, UV ozone radiation etc. makes PDMS surface produce hydroxyl, carboxyl, amino etc.
Reactive group, hydroxyl is contained on surface can pass through sulfonic acid chloride method, two imide methods and the activation of diisocyanate method, then by egg
White fixing.The activation of surface carboxyl groups can be carried out by hydroxysuccinimide and carbodiimides etc..Surface amino groups can be led to
Cross coupling agent such as diisocyanate, dialdehyde, epoxy resin etc. and the albumen coupling containing difunctional.Thus arrive promotion cell
Adhere to the purpose of growth.Kim et al. first passes through the method process PDMS surface that UV irradiates, then by poly D-Lys (Poly-
D-Lysin) coating is fixed on its surface, and cell experiment result shows, modified surface is conducive to mesenchymal stem cell
The differentiation of neuronal cell and propagation.
As in figure 2 it is shown, grafting modification:
Plasma grafting and UV surface cause polymerization to be all to radiate through plasma or ultraviolet to carry out surface active,
There is required for being further carried out grafting the polymer of modified function.Tatsuro et al., with propiophenone as optical initiator, uses
Ultraviolet light irradiates PDMS surface so that it is activation, and then causes 2-methyl-propen acyloxyethyl phosphatidylcholine (MPC) on surface
Polymerization, formed brush-like structure, such as Fig. 1.Research shows, increase light application time and monomer concentration can control the grafting of polymer
Density.Modified surface demonstrates good anti-protein adsorption and the performance of cell adhesion.
Body grafting modification is by chemical reaction, introduces specific group or polymer, i.e. in PDMS body
The monomer or the compound that add other during PDMS solidification in PDMS prepolymer and firming agent carry out copolymerization, thus in surface shape
Become specific functional group or change surface characteristic.
Surface silanization can be carried out at the various material surfaces of hydroxyl, this is because hydroxyl can be with siloxanes at table
Si-O-Si key is formed on face.With amino, mercaptan, the siloxanes of hydroxyl termination and the Silanization reaction on surface, by various function bases
Group brings surface into, thus causes surface grafting polymerization.
Bioactive molecule is modified, and has good by modes such as absorption, ionic bond or covalent bonds at PDMS surface grafting
The bioactive molecule of good biocompatibility, can improve the biocompatibility of material effectively.General conventional bioactive molecule
Including heparin (Heparin), aminoacid, phospholipid etc..
Shi Jia et al. compares at the poly-dopamine of PDMS surface auto polymerization (PDA-PDMS), coating polylysine (PLL-
PDMS) MG-63, being coated collagen Types I (COL-PDMS) and oxygen plasma process (ox-PDMS) four kinds of processing methods is thin
The adhesiving effect of born of the same parents.The PDMS surface processed all can preferably be sticked and sprawl, as shown in Figure 3.
Cell patterning techniques.Cell pattern most important application study exactly cell patterning after its biological nature and
Base mechanisms, controls the size of cell, shape and spatial arrangements in two-dimensional surface, provides new work for RESEARCH ON CELL-BIOLOGY
Tool.It is to be limited on the pattern of given shape by cell at all, in the middle of this process, how to allow cell adhesion at this pattern
Upper and good sprawling is the Major Difficulties of this technology.This technology mainly includes two kinds of methods, and one is to pass through surface
The method modified builds one layer of figuratum region that can allow cell adhesion, and other parts are modified by surface and allowed cell not allow
Easily adhere to, finally allow on the pattern sticking to design of cell selective；The second provides one layer to cell and can adhere to
The Physical barriers of patterning, this Physical barriers can allow and take away after cell adhesion again, does not the most allow cell sustain damage simultaneously.
Conventional cell patterning method mainly has some other methods such as photoetching process, Soft lithograph method, and inkjet printing.
Do brocade ripple et al. with PDMS (polydimethylsiloxane) as base material, by hydrosilylation at its surface grafting
PEG (allyl polyglycol), utilizes ultraviolet light lithographic technique to prepare chemical constituent at above-mentioned surface (PEG-PDMS) heterogeneous
Patterned surface topological structure.The i.e. PEG of exposure area is removed by high-octane Excimer UV photoengraving and exposes the end
The base material of layer, then being retained of unexposed portion, thus realize cell patterned distribution, as shown in Figure 4 and Figure 5.Need
It is noted that PEG has repulsive interaction to cell adhesion and protein adsorption, and expose substrate surface after exposing removal PEG
(PDMS), and the substrate surface that exposes etches further through the high-energy photon of ultraviolet light so that it is cell can be at exposure region
Territory grows, and cannot be at unexposed area apposition growth, it is achieved cell patterns.
Lee's cosmos et al. goes out to have the elastomeric stamp of patterning by Soft lithograph fabrication techniques, such as Fig. 6, and by area of the pattern
Through oxygen plasma treatment, then it is coated PEI (polylysine), so that cell can enter along the pattern being coated with PEI
Row apposition growth.
In terms of biological surface modification, above-mentioned technology is mainly with plasma treatment, ultraviolet radiation, grafting modification etc.
Chemical means is modified as main, has certain clean room demand, and is required to contact, with PDMS surface, the complexity carrying out being correlated with
Chemical reaction, is easily destroyed the surface chemical structure character in the non-modified region of PDMS.
In terms of patterning techniques, indoor conditions is required high by photoetching technique, and the apparatus expensive used.At light
Needing before quarter full wafer PDMS surface carries out related chemistry modification, that uses in photoetching process has covering of patterning simultaneously
Film, and control diffraction of light impact, it is long that this has resulted in pattern fabrication cycle, the feature of complex process.
Soft lithograph technology stems from photoetching technique, on this basis, has used again elastomeric stamp, so being called soft quarter
Erosion technology.Its essence of Soft lithograph is, utilizes expensive lithographic equipment and mask etc. to prepare micro-pattern that we need, leads to afterwards
Crossing the effect of intermediate medium, carry out simple and replicate accurately and shift, be used for improving micro-machined efficiency, reduction is processed into
This.But its fabrication cycle is long, need have figuratum mask, and the silicon plate after photoetching is as template, pour into a mould PDMS, then carry out
The demoulding, is finally also performed to the surface modification treatment being correlated with, and technique is sufficiently complex.
Summary of the invention
It is an object of the invention to propose a kind of method that biological adhesiveness is quickly improved on laser patterning PDMS surface, tool
Body, for utilizing laser action material surface modifying or forming surface micro-structure, is not affecting non-modified area surface configuration or chemistry
In the case of character, simplify PDMS surface modification step, pattern PDMS surface, be greatly improved preparation efficiency.
The technical solution adopted in the present invention: the side of biological adhesiveness is quickly improved on a kind of laser patterning PDMS surface
Method, can form micro structure by the PDMS surface after laser action, provides a good environment for cell attachment growth；Or
There is chemical modification or structurally-modified by the PDMS surface after laser action, thus improve biological adhesiveness.
Preferably, described micro structure can be with fixing protein, cell growth factor, enzyme and nucleic acid.
Preferably, laser instrument produces high energy pulse laser beam, and described high energy pulse laser beam is after reflecting mirror, logical
Cross convex lens to be focused to PDMS surface, radiate the high-energy and impact force action brought at described high energy pulse laser beam
Under, there is photothermy and photochemical action in PDMS surface, and instant vaporization or splash, thus form micro structure on PDMS surface.
Preferably, laser instrument produces high energy pulse laser beam, and described high energy pulse laser beam is after reflecting mirror, logical
Cross convex lens to be focused to PDMS surface, radiate the high-energy and impact force action brought at described high energy pulse laser beam
Under, there is chemical modification or structurally-modified in PDMS surface, thus improves biological adhesiveness.
Preferably, PDMS is positioned on three-dimensional fine work platforms, controls three by computer and kinetic control system
Tie up the movement in vertical direction of fine work platforms to adjust the distance between PDMS surface and laser spot；By computer and fortune
Autocontrol system controls the two dimensional motion of the horizontal direction of three-dimensional fine work platforms in the adjustable mode of speed, prepares PDMS
The required zone map modified in surface, thus in the case of not affecting non-modified region, pattern PDMS surface, and improve it
Biological adhesiveness.
Compared with prior art, the invention has the beneficial effects as follows: (1) laser action of the present invention is in PDMS surface modification or shape
Become micro structure, improve PDMS surface biological tack；(2) motion of three-dimensional fine work platforms vertical direction of the present invention adjusts
PDMS surface and the distance focused between laser；Three-dimensional fine work platforms, the two dimensional motion of horizontal direction, institute can be patterned
The PDMS surface of biological adhesiveness need to be improved；(3) present invention utilizes laser directly to act on PDMS surface, by controlling the energy of laser
The technological parameters such as amount, pulse recurrence frequency and focal plane make its surface modification or form surface micro-structure, can be in various degree
The biological adhesiveness on raising PDMS surface.And by the control of three-dimensional platform, regional area that can be complicated on surface is carried out
Patterning modification.Compared to existing technology, the present invention is the easiest quickly, more flexibility (adaptability) of operation；Prior art is mainly to change
Mode changes the biological adhesiveness on PDMS surface.Relevant chemical reaction or the side with grafting is there is by PDMS surface
Formula so that it is surface forms polar group, or at surface adsorption associated biomolecule decorating molecule, analog cell epimatrix attachment proteins is made
With, promote the cell adhesion on PDMS surface.And if modified at PDMS patterned surface, in addition it is also necessary to increase the side of mask
Formula avoids non-modified region to be affected；(4) present invention utilizes laser action PDMS surface, makes PDMS surface regional area shape
Become rough micro structure, or under conditions of corresponding laser photon energy be enough to destroy PDMS chemistry bond energy so that it is
After chemical bond cracking, reaction generates the new functional group that can promote cell attachment, i.e. surface generation chemical modification again.Laser
Biological adhesiveness is improved on effect PDMS surface, can be according to laser works such as different pulse energies, pulse overlap rate and defocusing amounts
Skill parametric variable produces change in various degree, makes surface processing efficiency be greatly improved；(5) three-dimensional fine work platforms is vertical
Distance between motion adjustable PDMS surface and the laser spot in direction, three-dimensional fine work platforms does the two dimension of horizontal direction
Motion, in the way of scanning, prepares the required pattern improving cell adhesiveness, thus is not affecting the situation in non-modified region
Under, the biological adhesiveness of modified regions needed for raising PDMS surface.Visible, for complicated regional area, flexibility (adaptability) of operation is relatively
Good.(6) present invention utilizes laser to interact with PDMS, can carry out the most in atmosphere, will not produce harmful gas or harmful
Waste material, is a science environmental protection, the process for modifying surface of sustainable development；(7) present invention need not at the chemical modification of complexity
Reason step, efficiency is high, patterning simplicity, and controllable property is good and environmental protection.
Accompanying drawing explanation
Fig. 1 is the Method And Principle figure that biological adhesiveness is quickly improved on the present invention a kind of laser patterning PDMS surface；
Fig. 2 is the PMPC schematic diagram at the Graft copolymerization on PDMS surface；
Fig. 3 is that the MG-63 cell after cultivating 24h sprawls microphotograph；
Fig. 4 is that PDMS membrane surface is grafted Polyethylene Glycol schematic diagram；
Fig. 5 is ultraviolet light etching preparation PDMS-PEG patterned surface schematic diagram；
Fig. 6 is the schematic diagram of the making of Soft lithograph Elastic seal；
Fig. 7 is in embodiment three, through laser action PDMS surface-pathlength trajectory diagram；
Fig. 8 is in embodiment three, and cell was cultivated after 72 hours, unmodified PDMS surface, left side and right side laser modified after
PDMS surface biological tack design sketch；
Fig. 9 is in embodiment three, after cell is cultivated 72 hours, and laser modified rear surface biological adhesiveness effect enlarged drawing.
Detailed description of the invention
Technical scheme is further illustrated below in conjunction with specific embodiment.
Embodiment one
As it is shown in figure 1, a kind of method that biological adhesiveness is quickly improved on laser patterning PDMS surface, pass through laser action
After PDMS surface can form micro structure, for cell attachment growth provide a good environment；
Described micro structure can be with fixing protein, cell growth factor, enzyme and nucleic acid.
Laser instrument (1) produces high energy pulse laser beam, and described high energy pulse laser beam is after reflecting mirror (4), logical
Cross convex lens (6) to be focused to PDMS (7) surface, radiate the high-energy and impact brought at described high energy pulse laser beam
Under power effect, there is photothermy and photochemical action in PDMS (7) surface, and instant vaporization or splash, thus on PDMS (7) surface
Form micro structure.
Particularly as follows: PDMS is positioned on three-dimensional fine work platforms, by computer (3) and kinetic control system (2)
Control the movement in vertical direction of three-dimensional fine work platforms (8) to adjust the PDMS (7) distance between surface and laser spot；Logical
Cross computer (3) and kinetic control system (2) controls the horizontal direction of three-dimensional fine work platforms (8) in the adjustable mode of speed
Two dimensional motion, prepare the required zone map modified in PDMS (7) surface, thus do not affecting the situation in non-modified region
Under, pattern PDMS (7) surface, and improve its biological adhesiveness.
Embodiment two
As it is shown in figure 1, a kind of method that biological adhesiveness is quickly improved on laser patterning PDMS surface, pass through laser action
After PDMS surface there is chemical modification or structurally-modified, thus improve biological adhesiveness；
Laser instrument (1) produces high energy pulse laser beam, and described high energy pulse laser beam is after reflecting mirror (4), logical
Cross convex lens (6) to be focused to PDMS (7) surface, radiate the high-energy and impact brought at described high energy pulse laser beam
Under power effect, there is chemical modification or structurally-modified in PDMS (7) surface, thus improves biological adhesiveness.
Particularly as follows: PDMS is positioned on three-dimensional fine work platforms, by computer (3) and kinetic control system (2)
Control the movement in vertical direction of three-dimensional fine work platforms (8) to adjust the PDMS (7) distance between surface and laser spot；Logical
Cross computer (3) and kinetic control system (2) controls the horizontal direction of three-dimensional fine work platforms (8) in the adjustable mode of speed
Two dimensional motion, prepare the required zone map modified in PDMS (7) surface, thus do not affecting the situation in non-modified region
Under, pattern PDMS (7) surface, and improve its biological adhesiveness.
Embodiment three
As it is shown in figure 1, a kind of method that biological adhesiveness is quickly improved on laser patterning PDMS surface,
(1) PDMS substrate makes: PDMS prepolymer mixes with the ratio of 10:1 with firming agent, fully stirs evenly 10 minutes；Will
Mixture pours mould into；Because being mixed into more air when stirring evenly, vacuum suction half an hour need to be carried out with evacuator；Again will mixing
Thing puts into the drying baker of level, arranges temperature 65 degrees Celsius, accelerates solidification and obtains PDMS substrate in 4 hours；Take out PDMS substrate also
Seal up for safekeeping.
(2) laser instrument is the ultraviolet pulse laser of 355nm wavelength, opens laser instrument, and laser instrument (1) produces high energy arteries and veins
Rushing laser beam, described high energy pulse laser beam, after reflecting mirror (4), is focused to PDMS (7) by convex lens (6)
Surface, under described high energy pulse laser beam radiates the high-energy and impact force action brought, there is photo-thermal in PDMS (7) surface
Effect and photochemical action, and instant vaporization or splash, thus micro structure is formed on PDMS (7) surface；It is specially regulation Z axis to make to swash
Optical focus is positioned at PDMS (7) surface, and laser energy density is set as 11.8J/cm2, and width sets is 10 μ s, and repetition rate sets
It is set as 160mm/s for 30kHz, XY platform movement velocity.Controlled three-dimensional micro-by computer (3) and kinetic control system (2)
The movement in vertical direction of thin work platforms (8) is to adjust the PDMS (7) distance between surface and laser spot；Pass through computer
And kinetic control system (2) controls two maintenance and operations of horizontal direction of three-dimensional fine work platforms (8) in the adjustable mode of speed (3)
The dynamic zone map preparing the required modification in PDMS (7) surface, thus in the case of not affecting non-modified region, patterning
PDMS (7) surface, and improve its biological adhesiveness；Draw a line specifically by X horizontal direction, then be spaced 10 μm toward Y-direction,
Then X horizontal reverse direction line, then Y-direction is spaced 10 μm, and X horizontal direction is rule ... circulates always thus forms a process
Laser modified plane pattern, such as Fig. 7, this pattern is used only as testing laser modified surface adhesion effect, and subsequent pattern can
Being changed according to research needs, other laser parameters are also variable
Testing its modified biological adhesiveness, the cell of tack experiment is 293t mouse renal epithelial cell, observes
Device is inverted microscope, observes such as Fig. 8.
From figure 8, it is seen that the surface after laser modified significantly improves biological adhesiveness, cell trends towards laser
Modified PDMS surface attachment growth.The cell on unmodified surface, left side is less, and is in coherent condition, and major part is the most dead
Die, it is impossible to sprawl growth.And laser modified rear surface, right side cell has the trend of gathering, but major part still keeps independent individual,
Put out the feelers at surface spreading apposition growth.For cell attachment complexion more can be seen, amplify inverted microscope multiplying power, such as Fig. 9.
For a person skilled in the art, can technical scheme as described above and design, make other each
Plant corresponding change and deformation, and all these changes and deforms the protection model that all should belong to the claims in the present invention
Within enclosing.
Claims (5)
1. the method that biological adhesiveness is quickly improved on a laser patterning PDMS surface, it is characterised in that: pass through laser action
After PDMS surface can form micro structure, for cell attachment growth provide a good environment；Or by after laser action
PDMS surface there is chemical modification or structurally-modified, thus improve biological adhesiveness.
The method that biological adhesiveness is quickly improved on a kind of laser patterning PDMS surface the most according to claim 1, it is special
Levy and be: described micro structure can be with fixing protein, cell growth factor, enzyme and nucleic acid.
The method that biological adhesiveness is quickly improved on a kind of laser patterning PDMS surface the most according to claim 1, it is special
Levy and be: laser instrument produces high energy pulse laser beam, and described high energy pulse laser beam, after reflecting mirror, passes through convex lens
It is focused to PDMS surface, under described high energy pulse laser beam radiates the high-energy and impact force action brought, PDMS
There is photothermy and photochemical action in surface, and instant vaporization or splash, thus form micro structure on PDMS surface.
The method that biological adhesiveness is quickly improved on a kind of laser patterning PDMS surface the most according to claim 1, it is special
Levy and be: laser instrument produces high energy pulse laser beam, and described high energy pulse laser beam, after reflecting mirror, passes through convex lens
It is focused to PDMS surface, under described high energy pulse laser beam radiates the high-energy and impact force action brought, PDMS
There is chemical modification or structurally-modified in surface, thus improves biological adhesiveness.
5. the method quickly improving biological adhesiveness according to a kind of laser patterning PDMS surface described in claim 1 or 4, its
It is characterised by: PDMS is positioned on three-dimensional fine work platforms, controls three-dimensional fine by computer and kinetic control system
The movement in vertical direction of work platforms is to adjust the distance between PDMS surface and laser spot；By computer and motor control
System controls the two dimensional motion of the horizontal direction of three-dimensional fine work platforms in the adjustable mode of speed, prepares PDMS surface institute
The zone map that need to modify, thus in the case of not affecting non-modified region, pattern PDMS surface, and it is attached to improve its biology
The property.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610753010.1A CN106239944A (en) | 2016-08-29 | 2016-08-29 | A kind of method that biological adhesiveness is quickly improved on laser patterning PDMS surface |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610753010.1A CN106239944A (en) | 2016-08-29 | 2016-08-29 | A kind of method that biological adhesiveness is quickly improved on laser patterning PDMS surface |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106239944A true CN106239944A (en) | 2016-12-21 |
Family
ID=57597206
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610753010.1A Pending CN106239944A (en) | 2016-08-29 | 2016-08-29 | A kind of method that biological adhesiveness is quickly improved on laser patterning PDMS surface |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106239944A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108478298A (en) * | 2018-03-01 | 2018-09-04 | 澳门大学 | A kind of planting body of polysaccharide coatings and preparation method thereof containing in combination with growth factor |
| CN108823165A (en) * | 2018-04-17 | 2018-11-16 | 广州波奇亚标准及检测技术有限公司 | It is a kind of to change the new method that surface wettability realizes three-dimensional cell cultivation based on laser |
| CN110466177A (en) * | 2019-02-23 | 2019-11-19 | 成都佛吉亚利民汽车部件系统有限公司 | A kind of moulding plasma surface processing device |
| CN110665071A (en) * | 2019-10-14 | 2020-01-10 | 中国科学院长春应用化学研究所 | Antibacterial and anticoagulant type coating, functional material with antibacterial and anticoagulant type coating and preparation method of functional material |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1473866A (en) * | 2003-05-22 | 2004-02-11 | 上海交通大学 | Surface treating method of polymer material for improving adherent cell growth |
| CN102242053A (en) * | 2011-04-01 | 2011-11-16 | 沈越 | Biochip with polymer three-dimensional nanostructure |
| CN105855726A (en) * | 2016-06-15 | 2016-08-17 | 广东工业大学 | Particle manufacturing equipment and application thereof |
-
2016
- 2016-08-29 CN CN201610753010.1A patent/CN106239944A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1473866A (en) * | 2003-05-22 | 2004-02-11 | 上海交通大学 | Surface treating method of polymer material for improving adherent cell growth |
| CN102242053A (en) * | 2011-04-01 | 2011-11-16 | 沈越 | Biochip with polymer three-dimensional nanostructure |
| CN105855726A (en) * | 2016-06-15 | 2016-08-17 | 广东工业大学 | Particle manufacturing equipment and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| ROBERTO OSELLAME, HUGO J.W.M.HOEKSTRA等: "Femtosecond laser microstructuring: an enabling tool for optofluidic lab-on-chips", 《LASERS & PHOTONICS REVIEWS》 * |
| 江树镇，郭钟宁，郑文书，黄红光: "生物芯片微流道的微细加工工艺", 《电加工与模具》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108478298A (en) * | 2018-03-01 | 2018-09-04 | 澳门大学 | A kind of planting body of polysaccharide coatings and preparation method thereof containing in combination with growth factor |
| CN108823165A (en) * | 2018-04-17 | 2018-11-16 | 广州波奇亚标准及检测技术有限公司 | It is a kind of to change the new method that surface wettability realizes three-dimensional cell cultivation based on laser |
| CN108823165B (en) * | 2018-04-17 | 2021-11-16 | 广州波奇亚标准及检测技术有限公司 | Novel method for realizing three-dimensional cell culture by changing surface wettability based on laser |
| CN110466177A (en) * | 2019-02-23 | 2019-11-19 | 成都佛吉亚利民汽车部件系统有限公司 | A kind of moulding plasma surface processing device |
| CN110665071A (en) * | 2019-10-14 | 2020-01-10 | 中国科学院长春应用化学研究所 | Antibacterial and anticoagulant type coating, functional material with antibacterial and anticoagulant type coating and preparation method of functional material |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106239944A (en) | A kind of method that biological adhesiveness is quickly improved on laser patterning PDMS surface | |
| Kapur et al. | Fabrication and selective surface modification of 3‐dimensionally textured biomedical polymers from etched silicon substrates | |
| Ross et al. | Surface engineering the cellular microenvironment via patterning and gradients | |
| Pereira et al. | 3D photo-fabrication for tissue engineering and drug delivery | |
| Zhang et al. | Femtosecond laser nanofabrication of hydrogel biomaterial | |
| US20120122222A1 (en) | Nanotopographic compositions and methods for cellular organization in tissue engineered structures | |
| US8048641B2 (en) | Micropatterning of biological molecules using laser ablation | |
| US20100167401A1 (en) | Stacked, patterned biomaterials and/or tissue engineering scaffolds | |
| CN105504327B (en) | A kind of biomaterial with the micro- patterns of 3D and preparation method thereof | |
| Biria et al. | Superhydrophobic microporous substrates via photocuring: coupling optical pattern formation to phase separation for process-tunable pore architectures | |
| CN109900642A (en) | A kind of submicron order miniature optical reactor and preparation method thereof | |
| Hamid et al. | Surface modification of SU‐8 for enhanced cell attachment and proliferation within microfluidic chips | |
| CA2980659A1 (en) | Artificial descemet's membrane | |
| CN109971706B (en) | Bionic bone unit structure capable of regulating and controlling mesenchymal stem cell paracrine and preparation method thereof | |
| Matsuda | Photoiniferter-driven precision surface graft microarchitectures for biomedical applications | |
| CN108823165B (en) | Novel method for realizing three-dimensional cell culture by changing surface wettability based on laser | |
| Jeong et al. | Generation of uniform agarose microwells for cell patterning by micromolding in capillaries | |
| Perez-Hernandez et al. | Contactless laser-assisted patterning of surfaces for bio-adhesive microarrays | |
| Matsuda et al. | Microfabricated surface designs for cell culture and diagnosis | |
| KR102499287B1 (en) | Plasma-assisted flexible multi-scale topographic patches for engineering cellular behavior and tissue regeneration | |
| Steuber et al. | Guided assembly, nanostructuring and functionalization with brushes of microscale polymer cubes for tailored 3-D cell microenvironments | |
| Zhu et al. | Foldable micropatterned hydrogel film made from biocompatible PCL‐b‐PEG‐b‐PCL diacrylate by UV embossing | |
| Rana et al. | Surface functionalization of biomaterials | |
| Yang et al. | Micropatterned cell‐repellent interface using femtosecond laser direct writing to engineer controlled cell organization | |
| Song et al. | Asymmetric multifunctional 3D cell microenvironments by capillary force assembly |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161221 |