CN106237382A - Promote CO2 laser weld pipe and preparation method thereof - Google Patents
Promote CO2 laser weld pipe and preparation method thereof Download PDFInfo
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- CN106237382A CN106237382A CN201610729307.4A CN201610729307A CN106237382A CN 106237382 A CN106237382 A CN 106237382A CN 201610729307 A CN201610729307 A CN 201610729307A CN 106237382 A CN106237382 A CN 106237382A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/222—Gelatin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3834—Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
- A61L2300/414—Growth factors
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/32—Materials or treatment for tissue regeneration for nerve reconstruction
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Abstract
The present invention relates to bioengineering field, be specifically related to a kind of rush CO2 laser weld pipe and preparation method thereof.In order to overcome the best problem of neurological functional recovery after current direct neural suture, the invention provides a kind of rush CO2 laser weld pipe.This CO2 laser weld pipe is the hollow pipe that the solution of the propylated gelatin of base is made.The growth that this rush CO2 laser weld pipe is neural provides a stable microenvironment.Be conducive to wound to recover, promote neural functional rehabilitation, close with nerve autograft function and be better than simple nerve trachea, there is good potential applicability in clinical practice.
Description
Technical field
The present invention relates to biological engineering and biomedical sector, be specifically related to promote CO2 laser weld pipe and preparation method thereof.
Background technology
Current various wound such as compressing, drawing-off, tear, the reason such as cut-out causes neural part or all of damage,
Thus nerve causes afunction and other nervous system diseasies.For there is the nerve of damage, neural neuron axon is suitable
Can regenerate in the case of when.By the development of the most micro-neurosurgery of neurosurgery, the reparation of nerve injury and regeneration are
It is possibly realized.Research at present and the CO2 laser weld technology applied clinically mainly have: directly stitching, neural transplantation (autotransplantation
Or heteroplastic transplantation) and repair by promoting CO2 laser weld pipe.Directly it coincide and be only limited to shorter neurotmesis (less than 5mm), no
Then can there is identical tension force, be unfavorable for neuranagenesis；Neural transplantation and rush CO2 laser weld pipe are mainly used in the long nerve blocked and repair
Multiple, but neural transplantation owing to being limited by donor, immunoreation etc., it is difficult to commonly use；CO2 laser weld pipe can provide
One good microenvironment promotes perineural reparation, has been obtained for large range of application in clinic.The most clinical
What middle use was most is a kind of silica gel tube, it is possible to guide the nerve tract proximate growth from neurologic defect position.But face
Bed result shows, owing to silica gel tube is that a kind of material that can not degrade needs second operation to take out, brings secondary nerve injury；Shape
Shape is difficult to mate completely with the damage location of patient；And biological activity is relatively low；Can not obtain very compared to neural transplantation patient
Good functional rehabilitation.
Summary of the invention
In order to solve the problems referred to above that this area exists, the invention provides a kind of rush CO2 laser weld pipe.
This rush CO2 laser weld pipe is that the solution of methyl-prop alkylene gelatin forms the hollow pipe that gel prepares.
Wherein, the tube wall of above-mentioned rush CO2 laser weld pipe is attached with fat stem cell.Fat it is attached with dry on described tube wall
Cell refers to that at least side tube wall is attached with fat stem cell.
Further, above-mentioned rush CO2 laser weld pipe is to inject the solution of methyl-prop alkylene gelatin to promote CO2 laser weld pipe mold
In prepare.
The present invention additionally provides the method that preparation promotees CO2 laser weld pipe simultaneously, it is characterised in that comprise the following steps:
A, the solution preparing methyl-prop alkylene gelatin respectively and rush CO2 laser weld pipe mold；
B, injecting in mould by the solution of methyl-prop alkylene gelatin, gel to be formed, mold removal, lyophilizing obtains promoting nerve
Repair pipe；
C, fat stem cell suspension is added drop-wise to step b prepare promote CO2 laser weld pipe wall on, hatch in incubator
0.5h～1h, makes cell fully be attached on rush CO2 laser weld pipe, to obtain final product.
Wherein, the concentration of above-mentioned methyl-prop alkylene gelatin solution is 5%～10%w/v.
Further, in said method step b, before the solution of methyl-prop alkylene gelatin injects mould, over cure need to be added
Acid ammonium to content is 0.1%～0.5%w/v and tetramethylethylenediamine is 0.05%～0.2%w/v to content, fully mixes
To mixed solution.In Ben, the w/v in invention is the concentration measurement unit chemically commonly used, and refers to every milliliter of material added
Quality, as 0.5% refers to that the solution of 1mL adds the amount of 5mg.
Wherein, in said method step b: the mixed solution obtained is injected rapidly and is placed into-20 DEG C～the mould of 0 DEG C
In tool；After fully reaction forms gel, at thaw at RT, mold removal.
Wherein, the rush CO2 laser weld pipe that said method step b obtains after taking out mould need to carry out frozen dried.Above-mentioned freezes
Dry-cure is to process to removing the moisture promoted in CO2 laser weld pipe.
Wherein, after said method step c is hatched in incubator, after neurocele adds culture medium, it is cultivated for 2～6
Hour obtain promoting CO2 laser weld pipe.
Wherein, the mould of above-mentioned rush CO2 laser weld pipe is to be printed the personalized rush CO2 laser weld pipe die indirectly prepared by 3D
Tool or metal promote CO2 laser weld pipe mold indirectly.
Wherein, the concentration of the fat stem cell suspension described in said method is 1 × 107～1 × 108Individual/mL.
The above-mentioned rush CO2 laser weld pipe prepared.
The beneficial effects of the present invention is: the present invention, by indirect 3D technology, is prepared for a kind of degradable gelatin and derives
Thing (GelMA) promotees CO2 laser weld pipe, and can add fat stem cell.Experiment shows, the rush CO2 laser weld of fat stem cell
Pipe can directional guide nerve growth, reduce scar tissue and neuromatous growth, it is provided that the micro-loop that a nerve growth is good
Border.The existence of fat stem cell can discharge rush nerve growth factor, accelerates perineural pattern and functional restoration.This
Bright GelMA promotees CO2 laser weld pipe and has good biocompatibility and certain mechanical strength, elapses over time, promotees nerve
Repair pipe can progressively decompose, it is to avoid neurocele is to neural follow-up compressing and causes inflammation, also avoids second operation, has very
Good potential applicability in clinical practice.
Accompanying drawing illustrates:
Fig. 1, by 3-dimensional software (soildwork) and 3D printer be prepared for promote CO2 laser weld pipe mold.
Fig. 2, the Physico-Chemical Characterization of neurocele of preparation.A is prepared for promoting sciatic nerve according to the sciatic nerve of rat and repaiies
Multiple pipe.B is scanning electron microscope (SEM) photograph；C external degradation under collagenase effect；The subcutaneous degraded in D and E 1-8 week and H&E figure.By
The rush CO2 laser weld pipe that knowable to figure, we prepare is a kind of cavernous degradable biological material, and the biofacies having had
Capacitive.
Fig. 3, to doing the dyeing (A) of Live/dead at the fat stem cell promoting to have cultivated 2 days on CO2 laser weld pipe, Luo Dan
Bright phalloidin (B).C figure is the SEM figure after fat stem cell is cultivated 2 days in culture dish, and D figure is that fat stem cell exists
Promoting the SEM figure after cultivating 2 days in CO2 laser weld pipe, E figure is calculated at culture dish by different time points and promotees CO2 laser weld pipe
The vegetative state of middle cell.F figure and promotees to cultivate neurotrophy two days later in CO2 laser weld pipe at culture dish for fat stem cell
The release conditions of the factor.As can be seen from the figure fat stem cell can grow in GelMA promotees CO2 laser weld pipe and breed.
Fat stem cell secretory nerve trophic factors can be promoted promoting CO2 laser weld pipe.
2 after Fig. 4, different operation group, the sciatic nerve index of 4,8,16 weeks.Promote the addition of CO2 laser weld pipe as seen from the figure
Be conducive to neurological functional recovery, and plus the functional restoration of energy accelerator nerve after fat stem cell.The explanation of this figure promotees nerve
Repair pipe and can promote the functional restoration of nerve plus fat stem cell.
4 after Fig. 5, different operation group, the sciatic nerve speed of 8,16 weeks.Promote adding of CO2 laser weld pipe as seen from the figure
Enter beneficially neurological functional recovery, and plus the functional restoration of energy accelerator nerve after fat stem cell.
Fig. 6, A and B perform the operation figure, produce 1cm on sciatic nerve, then connect rush CO2 laser weld pipe and (or promote nerve and repair
Multiple pipe+fat stem cell), C, D, E, F are after 16 weeks, the audio-visual picture of neural recovery state.(C sham operated rats, D is for for certainly
Somatic nerves is transplanted, and E is for promoting CO2 laser weld pipe, and F is for promoting CO2 laser weld pipe+fat stem cell group).
Fig. 7, A, B, C, D be respectively sham operated rats, nerve autograft group, promote CO2 laser weld pipe group, promote CO2 laser weld pipe+
The H&E figure of the gastrocnemius of fat stem cell group；E is the average diameter of the meat fiber of different group.F is experiment operation group and vacation
The weight in wet base ratio of the gastrocnemius of operation group.
Detailed description of the invention:
The present invention, by indirect 3D technology, is prepared for a kind of degradable gelatine derivative (GelMA) and promotees CO2 laser weld pipe,
Also can be nerve trachea, and fat stem cell can be added.
This CO2 laser weld pipe is that the solution of methyl-prop alkylene gelatin forms the hollow pipe that gel prepares.Above-mentioned rush CO2 laser weld
Pipe promotees to prepare in CO2 laser weld pipe mold for being injected by the solution of methyl-prop alkylene gelatin.Wherein, this rush CO2 laser weld pipe is extremely
Few side tube wall is attached with fat stem cell.
This preparation promotees CO2 laser weld pipe and specifically can be prepared as follows:
A, the solution preparing methyl-prop alkylene gelatin respectively and rush CO2 laser weld pipe mold；
B, injecting in mould by the solution of methyl-prop alkylene gelatin, gel to be formed, mold removal, lyophilizing obtains promoting nerve
Repair pipe；
C, fat stem cell suspension is added drop-wise to step b prepare promote CO2 laser weld pipe wall on, hatch in incubator
0.5h～1h, makes cell fully be attached on rush CO2 laser weld pipe, to obtain final product.
The concentration of methyl-prop alkylene gelatin solution is that the intensity making formation gel properly and can drop in the suitable time
Solve.The concentration of 5%～10%w/v disclosure satisfy that needs.
Further, in said method step b, before the solution of methyl-prop alkylene gelatin injects mould, over cure need to be added
Acid ammonium and tetramethylethylenediamine, obtain mixed solution, injected rapidly in mould by the mixed solution obtained, and is formed solidifying with reaction
Glue.The effect of APS is to produce free radical, makes the carbon-carbon double bond in GelMA react.And TEMED play promotion APS be produced from by
Base, accelerates the effect of reaction.Mould is typically placed under-20 DEG C～0 DEG C of environment, after fully reacting formation gel, in room temperature solution
Freeze, mold removal.
The rush CO2 laser weld pipe obtained after taking out mould preferably carries out frozen dried, removes as far as possible and promotees in CO2 laser weld pipe
Moisture.
After step c is hatched in incubator, it is also possible to add culture medium on rush CO2 laser weld pipe, be cultivated for 2～6 hours
Obtain cellularised rush CO2 laser weld pipe.This like cell adheres to more preferably on tube wall, and activity can be higher.
Preparation promotees the various conventional mould that the mould of CO2 laser weld pipe can be this area.Wherein, in order to use target
Neural shapes more mates, and mould can be selected for being printed by 3D between the personalized rush CO2 laser weld pipe mold indirectly prepared or metal
Connect rush CO2 laser weld pipe mold.
The concentration of the fat stem cell suspension used during preparation is typically adjusted to 1 × 107～1 × 108Individual/ml.
Additionally, present invention also offers the above-mentioned rush CO2 laser weld pipe prepared.
Below by way of detailed description of the invention, the present invention is carried out further description.
Embodiment one, structure promote CO2 laser weld pipe mold
Promote CO2 laser weld pipe mold by 3D printer Design and preparation, and then rush CO2 laser weld pipe can be prepared.
First rat sciatic nerve diameter is measured in this is tested, the rat sciatic nerve diameter of 200～250g
About 1.2～1.3mm, it is contemplated that the factor such as neural swelling after peripheral nerve injury, for the rush god of Rats'Sciatic Nerve Injury customization
Repaired pipe about internal diameter is 1.5mm, and catheter outer diameter is 4mm (pipe thickness is 1.25mm).Gone out by SolidWork software design
And use desktop printer (TD ARTIST) print and promote the mould that CO2 laser weld pipe mates, see Fig. 1.
Embodiment two present invention promotees the preparation of CO2 laser weld pipe
1, the gelatin (GelMA) of methyl-prop alkylene is prepared
(1), at 60 DEG C, by complete with the concentration of 10% (W/V) for type A gelatin (purchased from Sigma company, article No.: G1890)
It is dissolved in phosphate buffer (PBS)；
(2), it is added dropwise over methacrylic anhydride so that it is concentration is 20% (v/v), fully reacts 1 hour at 50 DEG C；
(3), dilute with the PBS (40 DEG C) of 5 times of volumes to terminate above-mentioned reaction；
(4), product is dialysed 2 weeks at 40 DEG C, then lyophilizing in freeze dryer, preserve at-20 DEG C.
2, preparation personalization promotees CO2 laser weld pipe
(1), at 60 DEG C, being completely dissolved in distilled water by GelMA, make the solution of 5% (w/v), the solution of i.e. every 1mL is molten
Solve 50mg GelMA；
(2), by the GelMA being completely dissolved it is placed in 5 minutes on ice, is subsequently adding Ammonium persulfate. (APS；Public purchased from Sigma
Department) to 0.5% (w/v) and tetramethylethylenediamine (TEMED；Purchased from Sigma company) to 0.1% (w/v), fully mix；
(3), said mixture is injected rapidly in the mould being placed into-20 DEG C of refrigerators；
(4), frozen 24 hours, fully thaw, the effective distilled water of CO2 laser weld will be promoted after mold removal the most clean, then
Lyophilizing also saves backup at-20 DEG C.
3, the sign of CO2 laser weld pipe is promoted
In aforementioned manners formation determination rat right sciatic nerves and design and be prepared for promote CO2 laser weld pipe, then to system
Standby rush CO2 laser weld pipe carries out characterizing (result sees Fig. 3).
Result shows
(1), scanning electron microscope show that the rush CO2 laser weld pipe of preparation has pore structure, the size in hole about 167 ±
13.5um, porosity 89.3% ± 1.04%, swelling ratio 9.47 ± 1.00；
(2) by being placed on promoting CO2 laser weld pipe in the Type Ⅱ collagen enzyme of 1mg/mL, by neural at different time points
The quality of pipe compares with the quality of original neurocele, finds to promote CO2 laser weld pipe the most about 20 hours degradable.So
After will to promote CO2 laser weld pipe heeling-in subcutaneous at rat back, at different time points by the H&E picture at heeling-in position and naked eyes
Observe, find that promoting the CO2 laser weld pipe the most degradable time is 2～4 months.
(3), the rush CO2 laser weld pipe heeling-in dorsal sc to rat of fat stem cell will not be added, 1,2,4,8
The neurocele taking off heeling-in week does H&E dyeing, and result shows, the surrounding promoting CO2 laser weld pipe only has a small amount of inflammatory cell.
Illustrate that the inflammatory reaction that this rush CO2 laser weld pipe causes is less, there is good histocompatibility.
4, cellularised rush CO2 laser weld pipe is prepared
On the basis of above-mentioned steps 3, the present invention attempts promoting to adhere on CO2 laser weld pipe fat stem cell, adhering mode
Using new mode, and the mode of dropping prepares cellularised rush CO2 laser weld pipe, result sees Fig. 4.
Concretely comprise the following steps:
(1), from rat groin fatty tissue isolated fat stem cell, complete medium (DMEM low sugar+
10% hyclone) middle cultivation so that it is external abundant amplification；
(2), gather in the crops fat stem cell, take the rush CO2 laser weld pipe prepared before part, promote CO2 laser weld pipe at each
Upper dropping 30 μ l is altogether containing 1 × 106The cell suspension of fat stem cell is hatched 1 hour in incubator, makes cell fully be attached to
Promote on CO2 laser weld pipe；
(3), it is further continued for cultivating completely 3 hours by the rush CO2 laser weld pipe containing fat stem cell, uses pipettor to add
The DMEM fluid medium (comprising the hyclone of 10%, buy in Invitrogen company) of 500uL, makes fat stem cell fill
Divide and attach growth, obtain cellularised rush CO2 laser weld pipe.
Embodiment three, the present invention promote the vivo efficacy test of CO2 laser weld pipe
Choose adult rat (body weight 200～250g) and build model, assess rush CO2 laser weld pipe and repair peripheral nerve.
Rat is divided into 4 groups and accepts functional assessment, respectively sham operated rats, nerve autograft group, rush CO2 laser weld pipe
Group, rush CO2 laser weld pipe+fat stem cell group.
Operation technique is anaesthetized for rat is carried out lumbar injection chloral hydrate (0.5 milliliter/100 grams).After rat
Body hair is removed, and cuts 1 millimeter of wound parallel with femur, separated by biceps brachii m., expose sciatic nerve, hands at sciatic nerve
Art position is about at distance furcal nerve 5mm.
Sham operated rats does not cut off nerve；Nerve autograft group is for cutting off seam respectively at distance furcal nerve 5mm～15mm
Close；Promote CO2 laser weld Guan Zuwei and at distance furcal nerve 5mm～15mm, cut off the nerve of 1cm, then by fat-free stem cell
Rush CO2 laser weld pipe seam be combined in two ends；Promoting CO2 laser weld pipe+fat stem cell group is at distance furcal nerve 5mm～15mm
Cut off the nerve of 1cm, be then combined in two ends by having added the rush CO2 laser weld pipe seam being attached with fat stem cell.
By histology, sciatic nerve index, the mode such as electro physiology detects each group of index, result see Fig. 4～Fig. 7 and
Accompanying drawing explanation.By interpretation, prolongation over time, the rat of fat stem cell+rush CO2 laser weld pipe is used to exist
The sciatic nerve index of functional assessment and nerve conduction velocity all become closer to nerve autograft group, and this rush nerve is described
Repair pipe and there is in zoopery preferable result, have good application prospect clinically.
Claims (10)
1. promote CO2 laser weld pipe, it is characterised in that be to be formed, by the solution of methyl-prop alkylene gelatin, the hollow pipe that gel prepares.
Rush CO2 laser weld pipe the most according to claim 1, it is characterised in that: it is attached with fat stem cell on tube wall.
Rush CO2 laser weld pipe the most according to claim 1, it is characterised in that: for the gelatin solution of methyl-prop alkylene is injected
Promote CO2 laser weld pipe mold prepares.
4. the method promoting CO2 laser weld pipe described in preparation any one of claims 1 to 3, it is characterised in that comprise the following steps:
A, the solution preparing methyl-prop alkylene gelatin respectively and rush CO2 laser weld pipe mold；
B, injecting in mould by the solution of methyl-prop alkylene gelatin, gel to be formed, mold removal, lyophilizing obtains promoting CO2 laser weld
Pipe；
C, fat stem cell suspension is added drop-wise to step b prepare promote CO2 laser weld pipe wall on, hatch in incubator 0.5h～
1h, makes cell fully be attached on rush CO2 laser weld pipe, obtains cellularised rush CO2 laser weld pipe.
Method the most according to claim 4, it is characterised in that: the concentration of the solution of described methyl-prop alkylene gelatin is
5%～10%w/v.
Method the most according to claim 4, it is characterised in that: in the method for described step b, at methyl-prop alkylene gelatin
Solution injects before mould, and need to add Ammonium persulfate. to content is 0.1%～0.5%w/v and tetramethylethylenediamine is to content
0.05%～0.2%w/v, fully it is uniformly mixed so as to obtain mixed solution.
Method the most according to claim 6, it is characterised in that: the mixed solution obtained is injected rapidly be placed into-
In the mould of 20 DEG C～0 DEG C；After fully reaction forms gel, at thaw at RT, mold removal.
Method the most according to claim 4, it is characterised in that: after described step c is hatched in incubator, outside neurocele
Face adds culture medium, is cultivated for 2～6 hours obtaining promoting CO2 laser weld pipe, and cultivation below is to cultivate in incubator.
Method the most according to claim 4, it is characterised in that: the concentration of described fat stem cell suspension is 1 × 107～1
×108Individual/mL.
10. the rush CO2 laser weld pipe prepared by the method described in any one of claim 4～7.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106729983A (en) * | 2016-12-30 | 2017-05-31 | 四川大学 | One kind promotees CO2 laser weld composite conduit and preparation method thereof |
| CN109395165A (en) * | 2018-12-21 | 2019-03-01 | 江阴司特易生物技术有限公司 | A kind of artificial composite nerve conduit and preparation method |
| CN115137884A (en) * | 2022-06-28 | 2022-10-04 | 中国科学院苏州生物医学工程技术研究所 | Injectable hydrogel for nerve injury repair and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106729983A (en) * | 2016-12-30 | 2017-05-31 | 四川大学 | One kind promotees CO2 laser weld composite conduit and preparation method thereof |
| CN109395165A (en) * | 2018-12-21 | 2019-03-01 | 江阴司特易生物技术有限公司 | A kind of artificial composite nerve conduit and preparation method |
| CN115137884A (en) * | 2022-06-28 | 2022-10-04 | 中国科学院苏州生物医学工程技术研究所 | Injectable hydrogel for nerve injury repair and preparation method thereof |
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