CN106237362B - A kind of preparation method of the liquid microbe deodorant of high concentration - Google Patents
A kind of preparation method of the liquid microbe deodorant of high concentration Download PDFInfo
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- CN106237362B CN106237362B CN201610830599.0A CN201610830599A CN106237362B CN 106237362 B CN106237362 B CN 106237362B CN 201610830599 A CN201610830599 A CN 201610830599A CN 106237362 B CN106237362 B CN 106237362B
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- edaphon
- deodorant
- extracting solution
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- pearl
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/01—Deodorant compositions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/01—Deodorant compositions
- A61L9/012—Deodorant compositions characterised by being in a special form, e.g. gels, emulsions
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- Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Treating Waste Gases (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
A kind of preparation method of the liquid microbe deodorant of high concentration of the present invention, belongs to field of microbial culture technology；The technical problem to be solved is that the features using edaphon, when being used as deodorant, preparation method of the present invention is proliferated the edaphon with deodorization functions to thousands of times, tens of thousands of times, it promotes deodorizing effect and extends the deodorization time, necessary deodorizing effect can be reached using a small amount of deodorant；Preparation method of the present invention allows the edaphon for resting on gelatinization bead interior to be proliferated thousands of to tens of thousands of times, improve deodorizing effect, effectively extend deodorant uses the time, plays the role of can be obtained by necessary deodorizing effect using a small amount of deodorant.
Description
Technical field
A kind of preparation method of the liquid microbe deodorant of high concentration of the present invention, belongs to field of microbial culture technology.
Background technique
There are adsorptivities deodorant and the stenches such as the active carbon of absorption stench of physical property as the deodorant of deodorization in the past
Ingredient chemically reacts, and supplements the chemically deodorant of stink odor, and there are also some known using to microorganism evil
The deodorant for the biology that smelly moneyization effect generates.
Wherein active carbon etc. utilizes the deodorant of physical property adsorption capacity, when it adsorbs odor pollutant saturation, effect
With regard to unobvious, and new deodorant is needed, chemically deodorant especially quick-acting, there is momentary effect, it is not long-term
Effect, and because of environmental pollution caused by using product to be also possible to.
The deodorant of biology has its quick-acting and can continue its effect, handles house according to humus deodorant
The methods of domestic animals manure, sewage sludge, and fertilizer is made.The quantity of contained microorganism is according to soil and micro- life in 1 gram of soil
The type of object has its upper limit, so being to have the maximum quantity of microorganism to limit when extracting microorganism from soil.Another party
Face, for deodorization efficiency also with the quantity of microorganism, the usage amount of deodorant is also directly proportional with the quantity of microorganism.The use of deodorant
Time is also also directly proportional to a certain extent with the quantity of microorganism, so the quantity of the microorganism contained in deodorant is also to get over
It is much better.
The generation of edaphon must be extracted from humus, so to ensure the corruption containing edaphon at any time
Matter is grown, the acquirement work of humus is also very inconvenient.
Summary of the invention
Overcome the deficiencies in the prior art of the present invention, the technical problem to be solved is that the features using edaphon, make
In use, preparation method of the present invention makes there is the edaphon of deodorization functions to be proliferated to thousands of times for deodorant, Shuo Wanbei is mentioned
It rises deodorizing effect and extends the deodorization time, necessary deodorizing effect, preparation of the invention can be reached using a small amount of deodorant
Method can provide inexhaustible edaphon.
In order to solve the above technical problems, the technical scheme adopted by the invention is as follows: a kind of system of liquid microbe deodorant
Preparation Method, comprising the following steps:
It a, is by weight that 1:2 is sufficiently mixed to obtain mixing by the humus of the edaphon containing deodorization functions and water
Liquid, gained mixed liquor stand 1 day；
B, twice by the mixed liquor for standing one day in step a filtering, coarse filtration is carried out using gauze for the first time, removes big
The soil of grain is filtered using filter paper for the second time, and gained filtrate is edaphon extracting solution, the acidity of this edaphon extracting solution
In pH2 to 3；
C, sodium hydrate aqueous solution is added in edaphon extracting solution and adjusts pH value, be adjusted to neutrality, while making soil
Precipitation of iron ions in microorganism extracting solution obtains neutral soil microorganism extracting solution；
D, the resulting neutral soil microorganism extracting solution of step c is filtered with filter paper, removes iron content precipitating, the extracting solution
Contain 10 in middle 1g6To 107Edaphon；
E, the sodium alginate aqueous solution for being 4% with mass fraction by the neutral soil microorganism extracting solution of step d after filtering
It is mixed by volume for 1:1, is sufficiently mixed stirring, makes its complete and homogeneous, obtained in the sodium alginate of collosol state and soil is micro-
Biological mixed liquor instills this mixed liquor in the metal cation aqueous solution containing 2 to trivalent that mass fraction is 2%, forms table
The catenate pearl of face solation, edaphon are wrapped in the inside of pearl, by the way that this pearl is obtained by filtration,
10 contained in the resulting every 1g of pearl6To 107Edaphon to get arrive liquid microbe deodorant.
The step e pearl being obtained by filtration is placed into the fluid nutrient medium containing 2 to trivalent metal cation, is mentioned
The concentration of edaphon is inside high beading to get the liquid microbe deodorant for arriving high concentration.
It is internal by the pearl after the fluid nutrient medium culture of 2 to trivalent metal cation through past embedding techniques
Edaphon be released after, gained edaphon directly according to above-mentioned steps e method preparation to get to take no
It exhausts, inexhaustible liquid microbe deodorant.
The metal cation aqueous solution for arriving trivalent containing 2 in step e is calcium chloride water, aluminum chloride aqueous solution.
Water used in step a is distilled water or ion exchange water.
Compared with prior art the invention has the advantages that.
Using the speciality of edaphon as the service condition of deodorant, the edaphon that deodorant uses be can be
Thousands of times or tens of thousands of times of microbial growth in natural soils improve deodorizing effect, and extend deodorant uses the time.Using few
The deodorant of amount can reach necessary deodorizing effect, can not only breed edaphon, can also generate inexhaustible
With endless edaphon enrichment procedure.
The edaphon for resting on gelatinization bead interior can be proliferated thousands of to tens of thousands of times, improve deodorizing effect, have
Effect extends the use time of deodorant, plays the work that can be obtained by necessary deodorizing effect using a small amount of deodorant
With.
Specific embodiment
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1
A kind of preparation method of the liquid microbe deodorant of high concentration, comprising the following steps:
It a, is by weight that 1:2 is sufficiently mixed mixed by the humus of the edaphon containing deodorization functions and distilled water
Liquid is closed, gained mixed liquor stands 1 day；
B, twice by the mixed liquor for standing one day in step a filtering, coarse filtration is carried out using gauze for the first time, removes big
The soil of grain is filtered using filter paper for the second time, and gained filtrate is edaphon extracting solution, the acidity of this edaphon extracting solution
In pH2；
C, sodium hydrate aqueous solution is added in edaphon extracting solution and adjusts pH value, be adjusted to neutrality, while making soil
Precipitation of iron ions in microorganism extracting solution obtains neutral soil microorganism extracting solution；
D, the resulting neutral soil microorganism extracting solution of step c is filtered with filter paper, removes iron content precipitating, the extracting solution
Contain 10 in middle 1g6Edaphon；
E, the sodium alginate aqueous solution for being 4% with mass fraction by the neutral soil microorganism extracting solution of step d after filtering
It is mixed by volume for 1:1, is sufficiently mixed stirring, makes its complete and homogeneous, obtained in the sodium alginate of collosol state and soil is micro-
Biological mixed liquor instills this mixed liquor in the aqueous solution containing calcium chloride that mass fraction is 2%, forms surface solation
Catenate pearl, edaphon are wrapped in the inside of pearl, by the way that this pearl is obtained by filtration
F, the step e pearl being obtained by filtration is placed into the fluid nutrient medium containing calcium chloride, improves an internal beading
The concentration of the inside edaphon of shape is to get the liquid microbe deodorant for arriving high concentration.
Embodiment 2
A kind of preparation method of the liquid microbe deodorant of high concentration, comprising the following steps:
A, the humus of the edaphon containing deodorization functions and ion exchange water are sufficiently mixed by weight for 1:2
Mixed liquor is obtained, gained mixed liquor stands 1 day；
B, twice by the mixed liquor for standing one day in step a filtering, coarse filtration is carried out using gauze for the first time, removes big
The soil of grain is filtered using filter paper for the second time, and gained filtrate is edaphon extracting solution, the acidity of this edaphon extracting solution
In pH 3；
C, sodium hydrate aqueous solution is added in edaphon extracting solution and adjusts pH value, be adjusted to neutrality, while making soil
Precipitation of iron ions in microorganism extracting solution obtains neutral soil microorganism extracting solution；
D, the resulting neutral soil microorganism extracting solution of step c is filtered with filter paper, removes iron content precipitating, the extracting solution
Contain 10 in middle 1g7Edaphon；
E, the sodium alginate aqueous solution for being 4% with mass fraction by the neutral soil microorganism extracting solution of step d after filtering
It is mixed by volume for 1:1, is sufficiently mixed stirring, makes its complete and homogeneous, obtained in the sodium alginate of collosol state and soil is micro-
Biological mixed liquor, it is 2% containing in aluminum chloride aqueous solution that this mixed liquor, which is instilled mass fraction, forms the string of surface solation
The pearl of pearl, edaphon are wrapped in the inside of pearl, by the way that this pearl is obtained by filtration.
F, the step e pearl being obtained by filtration is placed into the fluid nutrient medium containing 2 to trivalent metal ion, is mentioned
The concentration of high internal catenate internal edaphon is to get the liquid microbe deodorant for arriving high concentration.
The metal cation aqueous solution for arriving trivalent containing 2 in step e is calcium chloride water, aluminum chloride aqueous solution.
Water used in step a is distilled water or ion exchange ultrapure water.
Embodiment 3
A kind of preparation method of the liquid microbe deodorant of high concentration, comprising the following steps:
It a, is by weight that 1:2 is sufficiently mixed mixed by the humus of the edaphon containing deodorization functions and distilled water
Liquid is closed, gained mixed liquor stands 1 day；
B, twice by the mixed liquor for standing one day in step a filtering, coarse filtration is carried out using gauze for the first time, removes big
The soil of grain is filtered using filter paper for the second time, and gained filtrate is edaphon extracting solution, the acidity of this edaphon extracting solution
In pH2；
C, sodium hydrate aqueous solution is added in edaphon extracting solution and adjusts pH value, be adjusted to neutrality, while making soil
Precipitation of iron ions in microorganism extracting solution obtains neutral soil microorganism extracting solution；
D, the resulting neutral soil microorganism extracting solution of step c is filtered with filter paper, removes iron content precipitating, the extracting solution
Contain 10 in middle 1g7Edaphon；
E, the sodium alginate aqueous solution for being 4% with mass fraction by the neutral soil microorganism extracting solution of step d after filtering
It is mixed by volume for 1:1, is sufficiently mixed stirring, makes its complete and homogeneous, obtained in the sodium alginate of collosol state and soil is micro-
Biological mixed liquor, it is 2% containing in calcium chloride water that this mixed liquor, which is instilled mass fraction, forms the string of surface solation
The pearl of pearl, edaphon are wrapped in the inside of pearl, by the way that this pearl is obtained by filtration.
F, the step e pearl being obtained by filtration is placed into the fluid nutrient medium containing 2 to trivalent metal ion, is mentioned
The concentration of high internal catenate internal edaphon is to get the liquid microbe deodorant for arriving high concentration.
The metal cation aqueous solution for arriving trivalent containing 2 in step e is calcium chloride water, aluminum chloride aqueous solution.
Water used in step a is distilled water or ion exchange ultrapure water.
The present invention can be summarized with others without prejudice to the concrete form of spirit or essential characteristics of the invention.Therefore, nothing
By from the point of view of which point, the embodiment above of the invention can only all be considered the description of the invention and cannot limit invention,
Claims indicate the scope of the present invention, and above-mentioned explanation does not point out the scope of the present invention, therefore, with the present invention
The comparable meaning and scope of claims in any variation, be all considered as being included within the scope of the claims.
Claims (5)
1. a kind of preparation method of liquid microbe deodorant, which comprises the following steps:
It a, is by weight that 1:2 is sufficiently mixed to obtain mixed liquor by the humus of the edaphon containing deodorization functions and water, institute
It obtains mixed liquor and stands 1 day；
B, twice by the mixed liquor for standing one day in step a filtering, coarse filtration is carried out using gauze for the first time, removes bulky grain
Soil is filtered using filter paper for the second time, and gained filtrate is edaphon extracting solution, and the acidity of this edaphon extracting solution is in pH
2 to 3；
C, sodium hydrate aqueous solution is added in edaphon extracting solution and adjusts pH value, be adjusted to neutrality, while making the micro- life of soil
Precipitation of iron ions in object extracting solution obtains neutral soil microorganism extracting solution；
D, the resulting neutral soil microorganism extracting solution of step c is filtered with filter paper, removes iron content precipitating, 1g in the extracting solution
In contain 106To 107Edaphon；
E, the sodium alginate aqueous solution that the neutral soil microorganism extracting solution of step d after filtering is 4% with mass fraction is pressed into body
Product is sufficiently mixed stirring, makes its complete and homogeneous, obtain in the sodium alginate of collosol state and edaphon than being that 1:1 is mixed
Mixed liquor instills this mixed liquor in the metal cation aqueous solution containing divalent or trivalent that mass fraction is 2%, forms surface
The catenate pearl of solation, edaphon are wrapped in the inside of pearl, by the way that this pearl is obtained by filtration, by
10 contained in this every 1g of pearl generated6To 107Edaphon to get arrive liquid microbe deodorant.
2. a kind of preparation method of liquid microbe deodorant according to claim 1, which is characterized in that by step e mistake
It filters obtained pearl to be placed into the fluid nutrient medium of the metal cation containing divalent or trivalent, improves soil inside beading
The concentration of microorganism is to get the liquid microbe deodorant for arriving high concentration.
3. a kind of preparation method of liquid microbe deodorant according to claim 2, which is characterized in that will be by containing
Pearl after the fluid nutrient medium culture of divalent or the metal cation of trivalent is through past embedding techniques, internal edaphon
After being released, gained edaphon is directly inexhaustible to get arriving according to the method preparation of above-mentioned steps e, inexhaustible
Liquid microbe deodorant.
4. a kind of preparation method of liquid microbe deodorant according to claim 1, which is characterized in that in step e
Metal cation aqueous solution containing divalent or trivalent is calcium chloride water, aluminum chloride aqueous solution.
5. a kind of preparation method of liquid microbe deodorant according to claim 1, which is characterized in that institute in step a
Water is distilled water or ion exchange water.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610830599.0A CN106237362B (en) | 2016-09-19 | 2016-09-19 | A kind of preparation method of the liquid microbe deodorant of high concentration |
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| CN201610830599.0A CN106237362B (en) | 2016-09-19 | 2016-09-19 | A kind of preparation method of the liquid microbe deodorant of high concentration |
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| CN106237362A CN106237362A (en) | 2016-12-21 |
| CN106237362B true CN106237362B (en) | 2019-03-29 |
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| CN111282987B (en) * | 2020-02-28 | 2022-03-29 | 中科鼎实环境工程有限公司 | Inhibitor for treating peculiar smell of polluted soil and treatment method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5279963A (en) * | 1991-04-18 | 1994-01-18 | Hobby Michael M | System for the decontamination of a contaminated gas |
| CN103212287A (en) * | 2013-04-15 | 2013-07-24 | 北京城市排水集团有限责任公司 | Method for producing high-efficiency biological deodorizing mixed stuffing |
| CN103656712A (en) * | 2013-11-20 | 2014-03-26 | 日照海韵环保生物科技发展有限公司 | Seaweed biological deodorant and preparation method thereof |
| CN104399098A (en) * | 2014-11-06 | 2015-03-11 | 临沂清宇环境资源工程设备有限公司 | Composite microbial deodorant and preparation method thereof |
-
2016
- 2016-09-19 CN CN201610830599.0A patent/CN106237362B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5279963A (en) * | 1991-04-18 | 1994-01-18 | Hobby Michael M | System for the decontamination of a contaminated gas |
| CN103212287A (en) * | 2013-04-15 | 2013-07-24 | 北京城市排水集团有限责任公司 | Method for producing high-efficiency biological deodorizing mixed stuffing |
| CN103656712A (en) * | 2013-11-20 | 2014-03-26 | 日照海韵环保生物科技发展有限公司 | Seaweed biological deodorant and preparation method thereof |
| CN104399098A (en) * | 2014-11-06 | 2015-03-11 | 临沂清宇环境资源工程设备有限公司 | Composite microbial deodorant and preparation method thereof |
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| CN106237362A (en) | 2016-12-21 |
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