CN106234415A - IDA mature polypeptide plant senescence accelerator, preparation method and application - Google Patents
IDA mature polypeptide plant senescence accelerator, preparation method and application Download PDFInfo
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- CN106234415A CN106234415A CN201610873610.1A CN201610873610A CN106234415A CN 106234415 A CN106234415 A CN 106234415A CN 201610873610 A CN201610873610 A CN 201610873610A CN 106234415 A CN106234415 A CN 106234415A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/84—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms six-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,4
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Abstract
The invention provides a kind of IDA mature polypeptide plant senescence accelerator and its preparation method and application, belong to plant senescence accelerator field, it is possible to promote the leaf senile of plant so that it is old and feeble acceleration, and without other extra bad phenotype.This this plant senescence accelerator function composition is IDA mature polypeptide, the aminoacid sequence Pro Ile Pro Pro Ser Ala Pro Ser Lys Arg His Asn of IDA mature polypeptide.This IDA mature polypeptide plant senescence accelerator includes: IDA mature polypeptide, 2 (N morpholine) ethyl sulfonic acid solution；Described 2 (N morpholine) ethyl sulfonic acid solution is that 2 (N morpholine) ethyl sulfonic acid monohydrate is dissolved in MS fluid medium.The present invention can be used for promoting plant senescence.
Description
Technical field
The present invention relates to plant senescence accelerator field, particularly relate to a kind of IDA mature polypeptide plant senescence accelerator and
Its preparation method and application.
Background technology
Leaf senile is the key factor affecting the economical character such as crop yield, quality.As a example by Nicotiana tabacum L., Nicotiana tabacum L. is weight
Want industrial crops, blade be its gather in the crops organ, along with Nicotiana tabacum L. enter late growth stage, blade enter ageing phase, Nicotiana tabacum L. top, in
Portion, lower blade mature period differ, and generally, the strategy that production uses different parts different times to gather completes, and delays
Collecting time；On the other hand, environmental factors is as being likely to result in Nicotiana tabacum L. " secondary is turned green " shadow close to the harvest time continuous rainy day
Ring and gather.Therefore, utilize old and feeble accelerator, can promote that plant leaf blade is old and feeble, can become by efficient solution different parts tobacco leaf of determining
Ripe degree is inconsistent and problem of turning green.
At present, the application controlling plant senescence is concentrated mainly on the regulation aspect to phytohormone, and auxin, cell divide
Splitting element etc. can suppress leaf senile, ethylene, abscisic acid can promote leaf senile；Artificially slow down Leaf senescence development, on the one hand can
To promote synthesis or the metabolism of hormone by reducing aging, synthesis or the metabolism of senescence inhibitory hormones on the other hand also can be increased.
The most wide and deep the carrying out of work of old and feeble relevant biological function explore.Arabidopsis 2-oxoglutarate relies on
Dioxygenase gene disappearance can make leaf senile postpone phenotype.After this gene overexpression induced by dexamethasone (DEX), can make to turn
Gene Arabidopsis leaf aging is in advance；Arabidopsis AtNAC family member's NAP gene overexpression can make blade aging ahead of time, and
NAP deletion mutant body then delay aging, equally, in Oryza sativa L., this gene also is able to affect leaf senile；In addition, plant
Thing tiny RNA, the aging of all controllable blades such as transcription factor family such as WRKY.But at present, the SAG reported
Really be applied directly in crop production also rarely has report.
Polypeptide is the small-molecule substance that the class in animal and plant body has regulating and controlling effect, and plant polypeptide take part in multinomial growth
Process, such as root meristem development, shoot apical meristem growth etc., but whether plant polypeptide participates in aging, have not been reported,
Mature polypeptide is through post-translational cleavage, modification by precursor protein, ultimately forms the functional molecular of the shortest aminoacid composition,
As part and the Receptor recognition being positioned on cell membrane, enabling signal is transduceed.Directly outer executing can to plant growing for mature polypeptide
Produce impact, such as the elongation of root.Mature polypeptide applied research on plant especially crop is little at present, the most will not
It applies the research in terms of promoting plant senescence.
Summary of the invention
It is an object of the invention to provide a kind of IDA mature polypeptide plant senescence accelerator, preparation method and application.Should
IDA mature polypeptide plant senescence accelerator can promote the leaf senile of plant so that it is old and feeble acceleration, and extra the most not without other
Good performance.
One aspect of the present invention provides IDA mature polypeptide plant senescence accelerator, this plant senescence accelerator function composition
For IDA mature polypeptide, the aminoacid sequence of IDA mature polypeptide is Pro-Ile-Pro-Pro-Ser-Ala-Pro-Ser-Lys-
Arg-His-Asn。
Preferably, described IDA mature polypeptide plant senescence accelerator includes: IDA mature polypeptide, 2-(N-morpholine) ethyl sulfonic acid
Solution；Described 2-(N-morpholine) ethyl sulfonic acid solution is that 2-(N-morpholine) ethyl sulfonic acid monohydrate is dissolved in MS fluid medium.
Preferably, in described IDA mature polypeptide plant senescence accelerator, the concentration of IDA mature polypeptide is 10-13 μm ol/
The concentration of L, 2-(N-morpholine) ethyl sulfonic acid be the pH of 2.8-3mmol/L, 2-(N-morpholine) ethyl sulfonic acid solution be 5.8-5.9.
It is furthermore preferred that in described IDA mature polypeptide plant senescence accelerator, the concentration of IDA mature polypeptide is 10 μm ol/L,
The concentration of 2-(N-morpholine) ethyl sulfonic acid be the pH of 2.8mmol/L, 2-(N-morpholine) ethyl sulfonic acid solution be 5.8.
Preferably, the IDA mature polypeptide plant senescence accelerator of the present invention also can add auxiliary agent, it is furthermore preferred that auxiliary agent is
Tween 20, addition is the 1-2 ‰ of old and feeble accelerator total amount.
Another aspect of the present invention provides the preparation method of IDA mature polypeptide plant senescence accelerator, concrete, the method
Comprise the steps:
(1) weighing IDA mature polypeptide powder to be dissolved in water or dimethyl sulfoxide solvent, being configured to concentration is 10-
The IDA mature polypeptide mother solution of 13mmol/L is standby；
(2) preparation MS fluid medium, weighs quantitative 2-(N-morpholine) ethyl sulfonic acid monohydrate, joins above-mentioned MS liquid
In body culture medium, being configured to the solution of 2.8-3mmol/L, regulation solution ph is 5.8-5.9, is sufficiently mixed to be uniformly dissolved and obtains
2-(N-morpholine) ethyl sulfonic acid solution；
(3) the IDA mature polypeptide mother solution that step (1) obtains is joined 2-(N-morpholine) ethyl sulfonic acid that step (2) prepares
In solution, IDA mature polypeptide mother solution and 2-(N-morpholine) ethyl sulfonic acid liquor capacity ratio is for 1:1000, and Glass rod stirs,
To IDA mature polypeptide plant senescence accelerator.
Preferably, in step (1), solvent is dimethyl sulfoxide；
Preferably, step (3) adds in IDA mature polypeptide plant senescence accelerator the tween 20 of 1-2 ‰, uses glass
Rod stirs.
Preferably, IDA mature polypeptide mother solution matching while using in step (1), if not using more than 40 days, the storage of-70 DEG C of mother solution
Deposit；If using within 40 days, the storage of-20 DEG C of mother solution, using 4 DEG C within one week and storing.
Another aspect of the present invention additionally provides above-mentioned IDA mature polypeptide plant senescence accelerator and is promoting plant senescence side
The application in face, is applied to the blade face of plant, concrete, and application process is: directly foliage spray or decline with IDA mature polypeptide plant
Old accelerator soak cotton balls smear blade face, plant leaf blade full extension can use period, it is preferred that Nicotiana tabacum L. can prematuration period
Foliage applying.
The beneficial effects of the present invention is: the IDA in the IDA mature polypeptide plant senescence accelerator of (1) present invention is ripe
Polypeptide synthetic is convenient, and it is convenient to obtain, and can synthesize in high volume；(2) preparation of the plant senescence accelerator of the present invention and use
Method is simple, and field is workable, needs not move through Special Training for the personnel of using, and operation is convenient, practical；(3) this
Bright plant senescence accelerator, on leaf senile impact appropriateness, does not results in untoward reaction, beneficial.
Accompanying drawing explanation
Fig. 1 is that on arabidopsis, IDA gene delection postpones leaf senile schematic diagram；
Fig. 2 is that the IDA mature polypeptide plant senescence accelerator of synthetic processes arabidopsis excised leaf result schematic diagram；
Fig. 3 is that the IDA mature polypeptide plant senescence accelerator of synthetic processes Nicotiana tabacum L. excised leaf result schematic diagram；
Fig. 4 is that IDA mature polypeptide plant senescence accelerator processes live body tobacco leaf result schematic diagram.
Detailed description of the invention
Technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described enforcement
Example is only a part of embodiment of the present invention rather than whole embodiments.Based on the embodiment in the present invention, this area is common
The every other embodiment that technical staff is obtained under not making creative work premise, broadly falls into the model of present invention protection
Enclose.
Embodiments providing a kind of IDA mature polypeptide plant senescence accelerator, including IDA mature polypeptide, IDA becomes
The aminoacid sequence of ripe polypeptide is Pro-Ile-Pro-Pro-Ser-Ala-Pro-Ser-Lys-Arg-His-Asn, and this plant declines
Old accelerator includes: 10-13 μm ol/L IDA mature polypeptide, 2.8-3mmol/L 2-(N-morpholine) ethyl sulfonic acid solution；Described 2-
(N-morpholine) ethyl sulfonic acid solution is that 2-(N-morpholine) ethyl sulfonic acid solution monohydrate is dissolved in MS fluid medium, 2-(N-morpholine)
The pH of ethyl sulfonic acid solution is 5.8-5.9.
It is understood that the concentration of IDA mature polypeptide can be 10,11,12,13 μm ol/L, 2-(N-morpholine) second sulphur
The concentration of acid solution can be 2.8,2.9, the pH of 3mmol/L, 2-(N-morpholine) ethyl sulfonic acid solution can be 5.8,5.9.
Concrete, in the above embodiment of the present invention, IDA mature polypeptide plant senescence accelerator also includes auxiliary agent, institute
Stating auxiliary agent can be the conven-tional adjuvants extended for blade face moistening and blade face in this area, and the auxiliary agent that the present invention adds is tween 20,
Addition is the 1-2 ‰ of IDA mature polypeptide plant senescence accelerator total amount, but it is understood that, this area is any commonly employed
Blade face used additives all can add thus promote the application of the IDA mature polypeptide plant senescence accelerator of the present invention, such as ability
The surfactant in territory, extension agent, wetting agent etc., addition is the conventional amount used of corresponding additive.
Another embodiment of the present invention provides the preparation method of IDA mature polypeptide plant senescence accelerator, concrete, should
Method comprises the steps:
S1 weighs IDA mature polypeptide powder and is dissolved in water or dimethyl sulfoxide solvent, and being configured to concentration is 10-
The IDA mature polypeptide mother solution of 13mmol/L is standby.
In this step, owing to dimethyl sulfoxide is more preferable to the solubility property of polypeptide, the molten of polypeptide can preferably be promoted
Solve, preferably promote that polypeptide provides corresponding function.(it is difficult in room temperature owing to storage temperature is had higher requirements by IDA mature polypeptide
Placement was more than 24 hours), therefore IDA mature polypeptide mother solution matching while using, if not using more than 40 days, the storage of-70 DEG C of mother solution；If
Use within 40 days, the storage of-20 DEG C of mother solution, use 4 DEG C within one week and store.
S2 prepares MS fluid medium, weighs quantitative 2-(N-morpholine) ethyl sulfonic acid monohydrate, joins above-mentioned MS liquid
In body culture medium, being configured to the solution of 2.8-3mmol/L, regulation solution ph is 5.8-5.9, is sufficiently mixed to be uniformly dissolved and obtains
2-(N-morpholine) ethyl sulfonic acid solution.
In this step, MS culture medium can not only fully dissolve 2-(N-morpholine) ethyl sulfonic acid, thus ensures in plant surface shape
Becoming certain infiltration type, the effect for polypeptide provides basis, and meanwhile, this fluid medium is also provided that corresponding big as solvent
Moderate-element nutrition, it is ensured that action effect.
2-(N-morpholine) ethyl sulfonic acid that the IDA mature polypeptide mother solution that step (1) is obtained by S3 joins step (2) prepared is molten
In liquid, IDA mature polypeptide mother solution and 2-(N-morpholine) ethyl sulfonic acid liquor capacity ratio is for 1:1000, and Glass rod stirs, and obtains
IDA mature polypeptide plant senescence accelerator.
Can add the tween 20 of 1-2 ‰ in this step, corresponding auxiliary agent can promote above-mentioned solution as soon as possible at plant table
Face carries out extending and permeating moistening, concrete, owing to the addition of IDA mature polypeptide is less, in actual mechanical process preferably
The solution of the step S1 solution of step S2 is diluted 1000 times, can be 10-13 μm ol/ by the concentration dilution of IDA mature polypeptide
L。
Another embodiment of the present invention provides this IDA mature polypeptide plant senescence accelerator in terms of promoting plant senescence
Application, application process is: directly foliage spray or soak cotton balls with IDA mature polypeptide accelerator and smear blade face, plant leaf blade
Full extension can use period, it is preferred that Nicotiana tabacum L. can prematuration period foliage applying.
The cotton balls spraying or soaking this IDA mature polypeptide plant senescence accelerator smears plant leaf blade, can make plant growing
On the premise of state is unaffected, promoting that plant leaf blade is old and feeble, the concordance being particularly conducive to the asynchronous old and feeble crop of blade is adopted
Receive, such as Nicotiana tabacum L..
In the IDA mature polypeptide plant senescence accelerator of above-described embodiment, IDA mature polypeptide synthetic is convenient, obtains just
Profit, can synthesize in high volume；Preparation and using method are simple, and field is workable, and it is special to need not move through for the personnel of using
Training, operation is convenient, practical；This plant senescence inhibitor, on leaf senile impact appropriateness, does not results in untoward reaction, especially exists
Possess on Nicotiana tabacum L. and significantly apply effect.
In order to become apparent from introducing the IDA mature polypeptide plant senescence accelerator that the embodiment of the present invention is provided in detail, under
Face will be described in conjunction with specific embodiments.
Embodiment 1
IDA mature polypeptide plant senescence accelerator, including: 10 μm ol/L IDA mature polypeptides, 2.8mmol/L 2-(N-
Quinoline) ethyl sulfonic acid solution；Described 2-(N-morpholine) ethyl sulfonic acid solution is that 2-(N-morpholine) ethyl sulfonic acid solution monohydrate is dissolved in MS
Fluid medium, the pH of 2-(N-morpholine) ethyl sulfonic acid solution is 5.8.
Preparation method is as follows:
(1) weighing IDA mature polypeptide powder to be dissolved in dimethyl sulfoxide solvent, being configured to concentration is 10mmol/L's
IDA mature polypeptide mother solution is standby；
(2) preparation MS fluid medium, weighs quantitative 2-(N-morpholine) ethyl sulfonic acid monohydrate, joins above-mentioned MS liquid
In body culture medium, being configured to the solution of 2.8mmol/L, regulation solution ph is 5.8, is sufficiently mixed to be uniformly dissolved and obtains 2-(N-
Morpholine) ethyl sulfonic acid solution；
(3) the IDA mature polypeptide mother solution that step (1) obtains is taken 40 μ L and joins the 2-(N-morpholine) that step (2) prepares
In ethyl sulfonic acid solution 40mL, Glass rod stirs, and obtains the IDA mature polypeptide plant senescence accelerator of 40mL.
Embodiment 2
IDA mature polypeptide plant senescence accelerator, including: 12 μm ol/L IDA mature polypeptides, 2.9mmol/L 2-(N-
Quinoline) ethyl sulfonic acid solution；Described 2-(N-morpholine) ethyl sulfonic acid solution is that 2-(N-morpholine) ethyl sulfonic acid solution monohydrate is dissolved in MS
Fluid medium, the pH of 2-(N-morpholine) ethyl sulfonic acid solution is 5.9.
Preparation method is as follows:
(1) weighing IDA mature polypeptide powder to be dissolved in dimethyl sulfoxide solvent, being configured to concentration is 12mmol/L's
IDA mature polypeptide mother solution is standby；
(2) preparation MS fluid medium, weighs quantitative 2-(N-morpholine) ethyl sulfonic acid monohydrate, joins above-mentioned MS liquid
In body culture medium, being configured to the solution of 2.5mmol/L, regulation solution ph is 5.9, is sufficiently mixed to be uniformly dissolved and obtains 2-(N-
Morpholine) ethyl sulfonic acid solution；
(3) the IDA mature polypeptide mother solution that step (1) obtains is taken 100 μ L and joins the 2-(N-morpholine) that step (2) prepares
In ethyl sulfonic acid solution 100mL, Glass rod stirs, and obtains the IDA mature polypeptide plant senescence accelerator of 100mL.
Embodiment 3
IDA mature polypeptide plant senescence accelerator, including: 13 μm ol/L IDA mature polypeptides, 3.0mmol/L 2-(N-
Quinoline) ethyl sulfonic acid solution；Described 2-(N-morpholine) ethyl sulfonic acid solution is that 2-(N-morpholine) ethyl sulfonic acid solution monohydrate is dissolved in MS
Fluid medium, the pH of 2-(N-morpholine) ethyl sulfonic acid solution is 5.8.
Preparation method is as follows:
(1) weigh IDA mature polypeptide powder to be dissolved in water, be configured to the IDA mature polypeptide that concentration is 13mmol/L female
Liquid is standby；
(2) preparation MS fluid medium, weighs quantitative 2-(N-morpholine) ethyl sulfonic acid monohydrate, joins above-mentioned MS liquid
In body culture medium, being configured to the solution of 2.9mmol/L, regulation solution ph is 5.8, is sufficiently mixed to be uniformly dissolved and obtains 2-(N-
Morpholine) ethyl sulfonic acid solution；
(3) the IDA mature polypeptide mother solution that step (1) obtains is taken 60 μ L and joins the 2-(N-morpholine) that step (2) prepares
In ethyl sulfonic acid solution 60mL, adding the tween 20 of 2 ‰, Glass rod stirs, and the IDA mature polypeptide plant obtaining 60mL declines
Old accelerator.
Embodiment 4
IDA mature polypeptide plant senescence accelerator, including: 13 μm ol/L IDA mature polypeptides, 3.0mmol/L 2-(N-
Quinoline) ethyl sulfonic acid solution；Described 2-(N-morpholine) ethyl sulfonic acid solution is that 2-(N-morpholine) ethyl sulfonic acid solution monohydrate is dissolved in MS
Fluid medium, the pH of 2-(N-morpholine) ethyl sulfonic acid solution is 5.8.
Preparation method is as follows:
(1) weigh IDA mature polypeptide powder to be dissolved in water, be configured to the IDA mature polypeptide that concentration is 13mmol/L female
Liquid is standby；
(2) preparation MS fluid medium, weighs quantitative 2-(N-morpholine) ethyl sulfonic acid monohydrate, joins above-mentioned MS liquid
In body culture medium, being configured to the solution of 2.9mmol/L, regulation solution ph is 5.8, is sufficiently mixed to be uniformly dissolved and obtains 2-(N-
Morpholine) ethyl sulfonic acid solution；
(3) the IDA mature polypeptide mother solution that step (1) obtains is taken 50 μ L and joins the 2-(N-morpholine) that step (2) prepares
In ethyl sulfonic acid solution 50mL, adding the tween 20 of 1 ‰, Glass rod stirs, and the IDA mature polypeptide plant obtaining 50mL declines
Old accelerator.
Experimental test
Process by carrying out IDA gene delection on arabidopsis, see Fig. 1.IDA gene in pattern dicotyledon arabidopsis
Deletion mutant IDA, with wild type control phenotype analytical under the same conditions: continuous light grows 25 days, plant material grown
State entirety Phenotypic Observation, result shows, the A figure of Fig. 1 can be seen that IDA deletion mutant body postpone leaf senile, meanwhile,
Utilize Ethanol Method to measure different materials identical leaf position (the 6th leaf position) chlorophyll content, analyze each material aging further and characterize ginseng
Number, the C figure of result such as Fig. 1, wild type is on the low side compared with IDA mutant chlorophyll content；It addition, continuous light to be grown the open country of 25 days
Raw type and mutant material have carried out value in measuring photosynthesis, and the D figure of Fig. 1 shows wild type relatively IDA mutant Photosynthetic Rate
On the low side, to sum up illustrate, when, after IDA gene delection, plant performance has gone out the symptom declined evening, imply that IDA polypeptide is promoting blade
Effect in terms of Shuai Lao.
Example 1 preparation IDA mature polypeptide plant senescence accelerator, process respectively according to application process arabidopsis,
The excised leaf of Nicotiana tabacum L. and the Live leaf of Nicotiana tabacum L..
Process 1: process Arabidopsis leaf: continuous light growth arabidopsis about 30 days, obtain with leaf position excised leaf (the
Six leaf positions), lain on filter paper, polypeptide reagent uniformly, fully spray, compares as 2.8mM 2-(N-morpholine) second meanwhile
Sulfonic acid (MES) processes, and MS aqueous solution processes, and observes phenotype.
Processing 2: Nicotiana tabacum L. is prosperous for a long time, process excised leaf, processing mode, for spraying, compares and processes 1 and compare same treatment
Process 3: Nicotiana tabacum L. is prosperous for a long time, process Nicotiana tabacum L. Live leaf, processing mode for smearing tobacco leaf with cotton balls, comparison with
Process 1 comparison same treatment, Phenotypic Observation.
Seeing Fig. 2, choose, with tweezers, identical leaf position (the 6th lotus throne leaf) Arabidopsis leaf that growing way is consistent, relatively regular puts
In culture dish, IDA mature polypeptide plant senescence accelerator processes in the way of sprinkling, adds comparison same way simultaneously and processes,
Placing 5-6 days under continuous light, result is it can be seen that figure A-B shows that IDA mature polypeptide plant senescence accelerator promotes arabidopsis
Leaf senile, figure C shows that IDA mature polypeptide can decline in evening of complementary ida mutant phenotype；Arabidopsis excised leaf sprayed after 3 days
The blade spilling IDA mature polypeptide plant senescence accelerator has obvious senescence phenotype, utilizes Ethanol Method to measure leaf green simultaneously and contains
Amount, figure D finds that the chlorophyll content in leaf blades compareing the process of relatively IDA mature polypeptide plant senescence accelerator is the most；
See Fig. 3, IDA mature polypeptide aging accelerator and promote that tobacco leaf is old and feeble, choose prosperous long-term Nicotiana tabacum L. growing way consistent
Blade, utilize IDA aging accelerator and comparison to spray, Fig. 3 A, C have visible senilism character after showing Nicotiana tabacum L. excised leaf 7 days,
Fig. 3 B shows, processes chlorophyll content with 10 μm ol/L IDA mature polypeptides and substantially reduces.To sum up showing, IDA mature polypeptide is planted
Thing aging accelerator has the effect promoting aging to Vitro Plant blade.
Seeing Fig. 4, the Ben Shi Nicotiana tabacum L. growing about 25 days under the conditions of 27 DEG C of continuous lights is material, and cotton balls soaks and exists respectively
In IDA mature polypeptide plant senescence accelerator working solution and comparison 5 minutes so that it is being impregnated with completely, the cotton balls that processed processes lives
Body tobacco leaf has visible senilism character (figure A) after 13 days: meanwhile, utilizes Ethanol Method mensuration to smear IDA mature polypeptide plant and declines
13,21 days tobacco leaf chlorophyll contents after old accelerator and comparison, result shows compared with the control, IDA mature polypeptide plant
Tobacco leaf chlorophyll content after old and feeble accelerator processes all reduces (figure B).To sum up show, IDA mature polypeptide plant senescence
Accelerator has the effect being obviously promoted aging to live plant blade.
Claims (10)
1.IDA mature polypeptide plant senescence accelerator, it is characterised in that this plant senescence accelerator function composition is that IDA is ripe
Polypeptide, the aminoacid sequence of IDA mature polypeptide is Pro-IIe-Pro-Pro-Ser-Ala-Pro-Ser-Lys-Arg-His-
Asn。
IDA mature polypeptide plant senescence accelerator the most according to claim 1, it is characterised in that described IDA mature polypeptide
Plant senescence accelerator includes: IDA mature polypeptide, 2-(N-morpholine) ethyl sulfonic acid solution；Described 2-(N-morpholine) ethyl sulfonic acid solution
It is dissolved in MS fluid medium for 2-(N-morpholine) ethyl sulfonic acid monohydrate.
IDA mature polypeptide plant senescence accelerator the most according to claim 2, it is characterised in that described IDA mature polypeptide
In plant senescence accelerator, the concentration of IDA mature polypeptide is 10-13 μm ol/L, and the concentration of 2-(N-morpholine) ethyl sulfonic acid is 2.8-
The pH of 3mmol/L, 2-(N-morpholine) ethyl sulfonic acid solution is 5.8-5.9.
IDA mature polypeptide plant senescence accelerator the most according to claim 3, it is characterised in that described IDA mature polypeptide
In plant senescence accelerator, the concentration of IDA mature polypeptide is 10 μm ol/L, and the concentration of 2-(N-morpholine) ethyl sulfonic acid is 2.8mmol/
The pH of L, 2-(N-morpholine) ethyl sulfonic acid solution is 5.8.
5. according to the IDA mature polypeptide plant senescence accelerator described in claim 1-4 any one, it is characterised in that described
IDA mature polypeptide plant senescence accelerator is possibly together with auxiliary agent.
IDA mature polypeptide plant senescence accelerator the most according to claim 5, it is characterised in that auxiliary agent is tween 20,
Addition is the 1-2 ‰ of old and feeble accelerator total amount.
7. the preparation method of the IDA mature polypeptide plant senescence accelerator described in claim 1-6 any one, its feature exists
In, the method comprises the steps:
(1) weighing IDA mature polypeptide powder to be dissolved in water or dimethyl sulfoxide solvent, being configured to concentration is 10-13mmol/L
IDA mature polypeptide mother solution standby；
(2) preparation MS fluid medium, weighs quantitative 2-(N-morpholine) ethyl sulfonic acid monohydrate, joins the training of above-mentioned MS liquid
Supporting in base, be configured to the solution of 2.8-3mmol/L, regulation solution ph is 5.8-5.9, is sufficiently mixed to be uniformly dissolved and obtains 2-
(N-morpholine) ethyl sulfonic acid solution；
(3) the IDA mature polypeptide mother solution that step (1) obtains is joined 2-(N-morpholine) the ethyl sulfonic acid solution that step (2) prepares
In, IDA mature polypeptide mother solution and 2-(N-morpholine) ethyl sulfonic acid liquor capacity ratio is for 1:1000, and Glass rod stirs, and obtains IDA
Mature polypeptide plant senescence accelerator.
The preparation method of IDA mature polypeptide plant senescence accelerator the most according to claim 7, it is characterised in that step
(1) in, solvent is dimethyl sulfoxide；Step (3) adds in IDA mature polypeptide plant senescence accelerator the tween 20 of 1-2 ‰,
Stir with Glass rod.
9. the application of the IDA mature polypeptide plant senescence accelerator described in claim 1-6 any one, it is characterised in that execute
For the blade of plant, application process is: directly foliage spray or soak cotton balls with IDL4 mature polypeptide accelerator and smear blade face,
Plant leaf blade full extension can use period.
The application of IDA mature polypeptide plant senescence accelerator the most according to claim 9, it is characterised in that described plant
For Nicotiana tabacum L., foliage applying Nicotiana tabacum L. prematuration period.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112400880A (en) * | 2020-11-27 | 2021-02-26 | 中国农业科学院烟草研究所 | IDL6 mature polypeptide plant aging promoter, and preparation method and application thereof |
| CN112400881A (en) * | 2020-11-27 | 2021-02-26 | 中国农业科学院烟草研究所 | IDL7 mature polypeptide plant aging promoter, and preparation method and application thereof |
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| CN112400881A (en) * | 2020-11-27 | 2021-02-26 | 中国农业科学院烟草研究所 | IDL7 mature polypeptide plant aging promoter, and preparation method and application thereof |
| CN112586500A (en) * | 2020-11-27 | 2021-04-02 | 中国农业科学院烟草研究所 | AtPep1 mature polypeptide plant senescence promoter, preparation method and application thereof |
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